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193 results about "Scatter correction" patented technology

Method for correcting and calibrating three-dimensional fluorescence data of colored soluble organic matters

The invention provides a method for correcting and calibrating three-dimensional fluorescence data of colored soluble organic matters. The method comprises the steps of setting measurement conditions such as three-dimensional fluorescence excitation, a wavelength generation range and a scanning speed; measuring a three-dimensional fluorescence spectrum of a sample; measuring a three-dimensional fluorescence spectrum of ultrapure water; performing Raman scattering correction by subtracting excitation-emission three-dimensional fluorescence spectrum of the ultrapure water from the excitation-emission three-dimensional fluorescence spectrum of the sample; assigning 0 to a triangular region of each three-dimensional fluorescence spectrum to perform Rayleigh scattering correction; performing calibration on the fluorescence intensity through the excitation wavelength of 350nm and the emission wavelength of 371-428nm of the ultrapure water; measuring an absorption spectrum of the sample to obtain the absorbency of the sample; performing inner filering effect correction on the three-dimensional fluorescence data according to the absorbency of the sample. According to the method disclosed by the invention, the influence of the inner filtering effect of the high-concentration sample on the fluorescence intensity can be eliminated without dilution of the sample.
Owner:NANJING INST OF GEOGRAPHY & LIMNOLOGY
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