The invention belongs to the technical field of
bletilla striata
polysaccharide preparation, and provides a high-purity
bletilla striata
polysaccharide preparation
quality control method, which comprises: constructing a
raw material spectrum-
polysaccharide content prediction model by using
Fourier transform near infrared spectroscopy and
partial least squares regression PLSR, and intelligently determining an initial material-
liquid ratio based on a predicted value; in the extraction stage, the concentration is monitored in real time through an online
refractometer, the ultrasonic power, the extraction time or the volume of a supplemented
solvent is dynamically adjusted, and it is ensured that the concentration and the
viscosity of an extracting solution are in the optimal interval; in the purification stage, the dosage of a purification
reagent and the heating rate are dynamically adjusted according to the real-time concentration; in the concentration stage, according to the real-time concentration and
viscosity, the vacuum degree is adjusted in a segmented mode, heating is controlled, and end point coking is prevented; according to the invention, on-line monitoring and closed-loop
feedback control of key parameters in the whole process are realized, and the purity, uniformity and production stability of the product are remarkably improved.