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Method for quickly on-line detection of traditional Chinese medicine Kuhuang injection effective ingredient using near infra red spectrum

A technology of near-infrared spectroscopy and active ingredients, which is applied in the detection field of traditional Chinese medicine injection ingredients, can solve the problems of complex sample processing and operation, and difficulty in online detection

Inactive Publication Date: 2007-11-28
常熟雷允上制药有限公司
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Problems solved by technology

The active ingredients of Kuhuang injection are mainly aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether, sophocarpine, and matrine. Traditional determination methods such as HPLC are complicated in sample processing and operation, and difficult to Realize online detection

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  • Method for quickly on-line detection of traditional Chinese medicine Kuhuang injection effective ingredient using near infra red spectrum

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Embodiment Construction

[0019] The method for fast on-line detection of active ingredients of Kuhuang injection by near-infrared spectroscopy, the detection steps are as follows: (1) Determination of active ingredients aloe-emodin, rhein, emodin, The content of chrysophanol, emodin methyl ether, sophocarpine, and matrine, and as a reference value, the specific method is as follows:

[0020] 1. Determination of content of aloe-emodin, rhein, emodin, chrysophanol and emodin methyl ether:

[0021] Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use glacial acetic acid: water (1:100) as mobile phase A, glacial acetic acid: methanol (1:100) as mobile phase B Phase, eluted according to the following linear gradient: 0min (15%B), 25min (67%B), 65min (100%B); column temperature 40°C; detection wavelength 254nm. The number of theoretical plates is not less than 15,000 based on the rhein peak.

[0022] Preparation of reference substance solution: Accur...

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Abstract

The invention discloses a method for measuring effective component content in kuhuang injection on line by near-infrared spectroscopy quickly. Effective components in kuhuang injection mainly comprise aloe-emodin, rhein, emodin, chrysophanol, physcion, sophocarpine and matrine so on. Near-infrared absorption spectroscopy collecting kuhuang injection make data of effective component content relate with near-infrared spectroscopy by HPLC method. Calibration model is built by partial least-squares regression method. Quick on-line measurement for effective component in injection can be accomplished. Studying result shows that near-infrared spectroscopy analyzing method can measure effective component in kuhuang injection effectively. The method is provided with simple pretreatment for sample, quick measurement and measuring multiple components at the same time. It can be used in on-line analysis and on-line quality control in tcm manufacture process.

Description

1. Technical field [0001] The invention relates to a method for detecting components of traditional Chinese medicine injections, in particular to a method for detecting active components of bitter yellow injections by using near-infrared spectroscopy, so as to realize rapid on-line detection of active components of traditional Chinese medicine injections. 2. Background technology [0002] Kuhuang injection originated from the famous prescription "Yinchenhao Decoction" in "Treatise on Febrile Diseases" written by Zhang Zhongjing, a famous medical sage in ancient my country. It is a national traditional Chinese medicine protection variety. Kuhuang injection clinically treats various types of liver diseases. It has rapid jaundice reduction and significant enzyme reduction. It can improve immunity and improve clinical symptoms at the same time, and has a high degree of safety. The active ingredients of Kuhuang Injection are mainly aloe-emodin, rhein, emodin, chrysophanol, emodin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N33/15G01N21/3577G01N21/359
Inventor 陈力建王恒斌刘雪松顾治平阙瑞艳
Owner 常熟雷允上制药有限公司
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