22 results about "Hplc method" patented technology

This invention proposes an online quality detection method for An Shen Bu Nao Liquid based on multispectral fusion. Addressing the bottlenecks of traditional detection techniques, such as long offline processing time, weak multi-index detection capabilities, and poor dynamic adaptability, this method achieves intelligent quality control throughout the entire production process. The device integrates ultraviolet-visible spectroscopy, near-infrared spectroscopy, and fluorescence spectroscopy modules. It constructs a multi-scale residual convolutional network to extract ultraviolet-visible spectral features, uses a two-way LSTM to analyze the fluorescence excitation-emission matrix, and employs dynamic baseline correction of the ultraviolet spectrum. This improves the detection accuracy of icariin content by 40% compared to traditional HPLC methods, and achieves a heavy metal residue detection limit of 0.05 ppm, providing an innovative solution for the intelligent production of traditional Chinese medicine preparations.

The invention belongs to the technical field of detection and analysis, and provides an HPLC detection method for taurocholic acid in snake gall bulbus fritilariae liquid, which comprises the following steps: collecting chromatograms of a test sample and a reference substance through HPLC, and judging whether a co-outflow phenomenon exists or not; if the co-outflow phenomenon occurs, interference proportion analysis is carried out, and main interferents are identified; the judged main interferents are removed; carrying out retention analysis to obtain a taurocholic acid retention rate, judging whether the taurocholic acid retention rate accords with expectation or not based on the taurocholic acid retention rate, and if not, judging whether a trailing signal is triggered or not; if the trailing signal is triggered, performing cause analysis to determine the cause of triggering the trailing signal; when the optimal methanol proportion is obtained, a comprehensive detection signal is triggered; if the comprehensive detection signal is triggered, performing comprehensive adaptation analysis of the HPLC detection method to obtain an adaptation index, and evaluating the comprehensive performance of the HPLC detection method according to the adaptation index; and the accuracy of HPLC taurocholic acid detection is effectively improved.

The application discloses a method for constructing a characteristic map of a traditional Chinese medicine and the characteristic map, wherein the method adopts an HPLC method to determine the content of an effective component, imperatorin, in Zhikang capsules, and constructs a high performance liquid chromatography characteristic map of the Zhikang capsules; 10 characteristic peaks are determined as the characteristic map, which are respectively from dragon's blood, rhubarb and Bai Zhi; the components from the dragon's blood are dracorhodin B (peak 2) and pterostilbene (peak 4); the components from the rhubarb are peak 1 (aloe-emodin), peak 3, peak 6 (emodin), peak 9 and peak 10; and the components from the Bai Zhi are imperatorin (peak 5), peak 7 and peak 8; the method covers polyphenols, anthraquinones and furanocoumarins and other main components, and constructs a quality evaluation system of the Zhikang capsules; the method has good specificity, precision, repeatability and solution stability, and can be used for standardizing the production of the Zhikang capsules and guaranteeing the uniformity and stability of the internal quality of products to provide comprehensive quality control.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a method for detecting N-fluorenylmethoxycarbonyl protected histidine and enantiomers thereof, which comprises the following steps: (1) preparing a system applicability solution; (2) injecting the system applicable solution into a high performance liquid chromatograph, and separating by adopting a chiral chromatographic column which takes amylose-tri (3, 5-dimethyl phenyl carbamate) covalently bonded porous silica gel microspheres as a stationary phase; (3) carrying out gradient elution by adopting a binary mobile phase system containing a mobile phase A and a mobile phase B; (4) obtaining a chromatogram map; and (5) calculating the contents of the N-fluorenylmethoxycarbonyl protected histidine and the enantiomer according to the chromatogram. The invention discloses a method for detecting Fmoc-His (Trt)-OH and an enantiomer thereof by an HPLC (High Performance Liquid Chromatography) method. The method has the advantages of very high sensitivity and separation degree, good repeatability and durability, simplicity in operation and stable and reliable result.

The invention discloses a method for determining the dissolution rate and the dissolution curve of purinol tablets. The dissolution rate of the allopurinol tablets is detected by high performance liquid chromatography instead of an ultraviolet spectrophotometer method used at present. According to the method, a chromatographic column is used as a silica gel bonded octadecylsilane column, an acid solution-organic phase is used as a mobile phase, and an isocratic elution mode is adopted for detection. According to the method for detecting the dissolution rate and the dissolution curve of the allopurinol tablet through the high performance liquid chromatography, detection can be easily and conveniently completed, specificity is high, accuracy is high, repeatability is good, application and linearity are achieved, and the dissolution rate of the allopurinol tablet and the dissolution amount of the dissolution curve can be efficiently and rapidly detected.

A computer implementation method for predicting the HPLC retention time of one or more compounds using a machine learning model is disclosed. In particular, the use of a machine learning model to identify a suitable set of HPLC method parameters for separating two or more compounds in a composition is disclosed. Related systems and products are also described.

This invention belongs to the field of chromatographic detection and analysis, and discloses a reversed-phase chromatographic analysis method for the purity of enterokinase protein. High-performance liquid chromatography (HPLC) is used to detect enterokinase in the sample solution. In this HPLC method, the chromatographic column is selected from reversed-phase chromatographic packing material C18 or C8, with a particle size of 3.5~5 μm and a pore size of 120~1000 Å. The mobile phase is selected as follows: an aqueous solution of heptafluorobutyric acid (HFA) is used as mobile phase A, and an acetonitrile solution of HFA is used as mobile phase B. This invention improves the separation of the main peak and subsequent impurities in the enterokinase stock solution by screening suitable mobile phase systems and column types. It also optimizes chromatographic parameters such as the proportion of modifier in the mobile phase, column temperature, sample loading, mobile phase gradient, and wavelength, ultimately achieving effective separation of the main peak and subsequent impurities in the enterokinase stock solution. Furthermore, the analysis time is short and the repeatability is good, providing an effective analytical method for the quality control of enterokinase stock solution.

The application discloses a method for determining the content of carvacol in compound fertilizer, and the method comprises gas phase determination and liquid chromatography, and the gas chromatography method for determining the content of carvacol in compound fertilizer comprises the following steps: S1, sample pretreatment; S2, matrix purification; S3, chromatographic analysis; and S4, quantitative calculation; in the application, a silica gel SPE column is used to replace a traditional diatomite column in the GC method, the polarity impurity adsorption is enhanced through ethyl acetate-hexane gradient activation, sodium chloride salting-out and EDTA complex metal ions are introduced in the HPLC method, the number of miscellaneous peaks in the GC chromatogram is reduced, the separation degree of the carvacol peak is improved, and the service life of the HPLC column is prolonged; the GC method is operated under the conditions of temperature control ultrasonic at 35±2 DEG C and full light avoidance; the HPLC method adopts room temperature methanol-water system extraction, and the recovery rate of the GC method is improved; the HPLC method avoids the 60 DEG C concentration requirement of acetonitrile extraction, and the light stability is improved.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to an HPLC (High Performance Liquid Chromatography) method for separating and determining nafamostat mesylate X1 and impurities thereof. The impurities comprise an impurity NAA-SM2, an impurity NAA-SM2a, an impurity NAA-SM2f, an impurity NAA-X1d, an impurity NAA-X1b, an impurity NAA-X1e, an impurity NAA-X1f and isopropyl p-aminobenzoate. According to the method, octadecyl silane bonded silica gel is adopted as a chromatographic column filler, a potassium hexafluorophosphate solution is adopted as a mobile phase A, acetonitrile is adopted as a mobile phase B, and nafamostat mesylate X1 and impurities thereof are separated through linear gradient elution; and then calculating the content of each impurity by adopting an external standard method and / or a principal component self-contrast method multiplied by a correction factor according to a chromatogram. The method has the characteristics of good separation degree, good durability, high sensitivity and good reproducibility.

The invention discloses an HPLC (High Performance Liquid Chromatography) method for detecting the content of two genotoxic impurities in phentolamine mesylate, belonging to the technical field of pharmaceutical analysis. The method comprises the following steps: 1) preparing a test solution and a reference solution; 2) setting high performance liquid detection conditions: adopting a chromatographic column taking octadecylsilane chemically bonded silica as a filler, taking an ammonium acetate buffer solution as a mobile phase A, taking acetonitrile as a mobile phase B, and carrying out gradient elution; 3) respectively and precisely sucking the test solution and the reference solution, injecting into a liquid chromatograph, and recording a chromatogram chart.According to the method, the two genotoxic impurities in the phentolamine mesylate can be rapidly, effectively, accurately and reliably separated and detected, the product quality of the phentolamine mesylate is improved, and the medication safety of a patient is further improved.

The invention provides a method for improving the accuracy of vancomycin plasma concentration monitored by HPLC (High Performance Liquid Chromatography) based on PDCA (Packet Data Convergence Analysis) circulation, and belongs to the technical field of quantitative analysis. When the clinical sample is abnormal, retesting the abnormal clinical sample to obtain a retesting result, and judging whether interference exists in the retesting sample or not according to the retesting result; when the existence of interference in the retest sample is still not determined, quantifying the vancomycin plasma concentration level in the retest sample based on a liquid chromatography-mass spectrometry method to obtain a liquid chromatography-mass spectrometry method quantitative result; and outputting the quantitative result of the liquid chromatography-mass spectrometry method when the quantitative result of the liquid chromatography-mass spectrometry method is inconsistent with the determination result of the high performance liquid chromatography. The method has the beneficial effects that through continuous application of PDCA circulation, the accuracy of vancomycin plasma concentration monitoring by the vancomycin HPLC analysis method is continuously improved, and the quality and accuracy of vancomycin TDM results are ensured.

The invention relates to a method for separating and determining a key starting material of clarcosinone and related compounds thereof by HPLC (High Performance Liquid Chromatography), belonging to the technical field of analytical chemistry. 17-hydroxy-21-acetoxyprogesterone and related compounds A-F of the key starting material of clarcosinone are separated and detected by liquid chromatography; a chromatographic column with octadecylsilane chemically bonded silica as a filler is adopted, an acetonitrile aqueous solution is used as a mobile phase A, an acetonitrile methanol solution is used as a mobile phase B, gradient elution is carried out, detection and analysis are carried out, and the content of each compound is calculated. According to the present invention, the key initial material of the clarcosinone can be effectively separated from the related impurity compounds, and the method has advantages of high sensitivity, high separation degree, good repeatability, good durability, simple operation and stable and reliable result, and has important significance on the realization of the clarcosinone drug quality control.

This invention provides a method for determining the content of lotus root nodes and related processed products by HPLC, comprising: A) dissolving and extracting the test sample raw material in a solvent to obtain a test solution; the test sample raw material includes lotus root nodes or related processed products; B) determining the test solution by high-performance liquid chromatography to obtain an HPLC chromatogram of the lotus root nodes or related processed products; the chromatographic conditions of the high-performance liquid chromatography are: a C18 column; mobile phase A is a methanol solution, mobile phase B is a 0.5% phosphoric acid aqueous solution, and gradient elution is performed; the gradient elution is specifically as follows: 0-1 min, phase A: 2%, phase B: 98%; 1-4 min, phase A... Phase A: 2% → 8%, Phase B: 98% → 92%; 4–6 min, Phase A: 8% → 0%, Phase B: 92% → 100%; 6–16 min, Phase A: 0%, Phase B: 100%; 16–30 min, Phase A: 10%, Phase B: 90%. This invention employs high-performance liquid chromatography (HPLC) with a methanol-0.5% phosphoric acid solution as the mobile phase for gradient elution. Using protocatechuic acid and p-hydroxybenzoic acid as reference standards, an HPLC method for determining the content of lotus root nodes and related processed products was established, providing more scientific technical means for controlling the medicinal quality of lotus root nodes and related processed products.

The invention discloses a comprehensive evaluation method for the quality of eucommia male flowers and application, belongs to the technical field of quality detection of traditional Chinese medicinal materials, and aims to solve the technical problems of single evaluation means, low efficiency and disjunction of quality and efficacy of the traditional eucommia male flowers. The method comprises the following five core steps: preparing a test solution, constructing and evaluating an UPLC (Ultra Performance Liquid Chromatography) exclusive fingerprint spectrum, quantifying HPLC (High Performance Liquid Chromatography) multi-index functional components, determining the functionality of total polyphenols and total flavonoids and carrying out three-dimensional synergistic judgment. Efficient separation of 19 common characteristic peaks is achieved through customized UPLC chromatographic conditions, the relative retention time range of the characteristic peaks is limited by taking chlorogenic acid as a reference peak, and content limits of four core active ingredients such as aucubin and the like, total polyphenol and total flavone are set based on an efficacy threshold value; and meanwhile, a plurality of groups of defensive experiments are additionally arranged to verify system collaboration, industrial practicability and efficacy relevance. According to the method, three-dimensional management and control of overall qualification, precise quantification and efficacy association of the eucommia ulmoides male flowers are achieved, the UPLC fingerprint detection efficiency is improved by 61% compared with a traditional HPLC method, the authenticity identification accuracy rate reaches 100%, the method is suitable for large-scale application of the whole industrial chain, and a scientific and feasible technical scheme is provided for standardized management and control of the quality of the eucommia ulmoides male flowers.

This invention relates to a pre-detection method for oligosaccharide active components in cottonseed wastewater based on high-performance liquid chromatography (HPLC). The method involves selecting corresponding standards for the characteristic oligosaccharide active components contained in the cottonseed wastewater and preparing samples by dissolving them in a mobile phase solution. Conventional HPLC is used for separation and detection, with acetonitrile as mobile phase A and water as mobile phase B. A series of standard solutions are injected into the HPLC instrument, and single-factor experiments are conducted on factors such as column temperature, flow rate, and mobile phase ratio. Chromatograms are recorded and analyzed, and standard curves are plotted. Qualitative analysis is performed using retention time, and quantitative analysis is performed using the external standard-calibration curve method. The HPLC method established in this invention can qualitatively and quantitatively detect the content of oligosaccharide compounds in cottonseed wastewater and can simultaneously detect multiple oligosaccharide active components, providing a green, low-consumption, standardized method and detection guarantee for the detection of characteristic oligosaccharide active components in cottonseed processing wastewater and related products.

This invention discloses an HPLC method for the simultaneous determination of five components—protocatechuic acid, verbascoside, argentin, verbascoside, and argentin—in Compound Euonymus fortunei Capsules. The chromatographic conditions used are as follows: an Acclaim 120-C18 column (250 mm × 4.6 mm, 5 μm) is used, with acetonitrile-0.2% phosphoric acid solution as the mobile phase, gradient elution, and a flow rate of 1 ml / min. ‑1 The column temperature was 30℃, and the detection wavelength was 254nm. Under these chromatographic conditions, the linear ranges for the five components were: protocatechuic acid Y = 11726348.35x + 87150.17; (R 2 =0.9999), verbascoside Y = 27134X - 0.5781; (R 2 =0.9994), stigmoside Y = 29605X + 14.0030; (R 2 =0.9996), verbascoside Y = 51970X - 25.7380; (R 2 =0.9991), styracin Y = 61726X + 7.1802; (R 2 =0.9991). The chromatographic peaks of protocatechuic acid, verbascoside, gentianin, verbascoside, and gentianin were well separated, and the concentration and peak area showed a good linear relationship. This method is simple, rapid, accurate, and reproducible, and can provide a theoretical basis for the quality of Compound Euonymus fortunei Capsules.

This invention discloses an HPLC method for simultaneously determining the content of six active ingredients in Hericium erinaceus extract, belonging to the field of natural compound detection and analysis technology. The detection steps of this invention include: preparing a mixed standard solution using Hericium erinaceus ketone E, Hericium erinaceus ketone C, Hericium erinaceus ketone D, Hericium erinaceus D, ergosterol, and Hericium erinaceus C as reference standards; analyzing the mixed standard solution using high-performance liquid chromatography (HPLC); plotting standard curves for the six active ingredients with the concentration of the mixed standard solution as the abscissa and the peak area as the ordinate; determining the linear regression equation; and calculating the content of each active ingredient in the sample solution. The HPLC method for simultaneously determining six active ingredients in Hericium erinaceus extract established in this invention has good selectivity, high sensitivity, accuracy, and reliability, providing a new analytical method and basis for the quality analysis and quality control of Hericium erinaceus extract.

The application provides a method for detecting impurities of brivaracetam injection by using a non-salt mobile phase system combined with reversed-phase liquid chromatography, the detection method is detected by using an HPLC method, and the mobile phase system is a mixed solution of a phosphoric acid solution and acetonitrile, wherein in the mixed solution, the volume percentage of acetonitrile is 30%-45%, and the concentration of the phosphoric acid solution is greater than or equal to 0.05%; the chromatographic column is a chiral column containing tris(4-chloro-3-methylphenyl aminomethyl ester). By using the above detection method, the related substances and isomers of brivaracetam can be separated and determined in the same analysis method, and the blank excipients do not interfere with the detection of the above components.

This invention discloses an HPLC method for determining the ortho-O iron content in EDDHA-Fe chelates, comprising the following steps: S1. Sample pretreatment: S11. The sample is first extracted with an aqueous solution, followed by solid-liquid separation; S12. The liquid phase is purified by passing it through a strong acid cation exchange resin to obtain a purified solution; S13. EDTA salt is added to obtain a test solution; S2. HPLC detection: A C18 reversed-phase column is used, with gradient elution using mobile phase A and mobile phase B; mobile phase A is a 0.05-0.15% (w / w) aqueous solution of phosphoric acid with a pH of 2.5-3.0; mobile phase B is methanol. This invention, through optimized chromatographic conditions and innovative sample pretreatment, establishes a high-resolution, interference-resistant method for O-O detection, solving the problems of insufficient resolution, interference ion influence, and inaccurate quantification in existing methods.

The invention discloses a quality transmission quality control method in the whole process of preparing a milk nuclear drink. Comprising the following steps: collecting a milk nuclear drink concentrated solution, a centrifugate and a decoction sample; determining seven physical parameters including pH, refractive index, dynamic viscosity, conductivity, total amount of soluble solids, solid content and density of the sample, and constructing a physical fingerprint spectrum; the method comprises the following steps: determining the contents of seven index components including gallic acid, chlorogenic acid, loganin, paeoniflorin, ferulic acid, chicoric acid and icariin by adopting an HPLC (High Performance Liquid Chromatography) method, calculating the transfer rate of the seven index components in each process stage, establishing a chemical fingerprint spectrum, identifying common peaks and calculating the similarity; and analyzing the correlation between the physical parameters and the index components through a heat map. The multi-index evaluation mode of physical fingerprint spectrum-chemical fingerprint spectrum-multi-component content determination constructed by the invention can systematically evaluate the influence of the traditional Chinese medicine preparation process on the overall quality, and provides a scientific basis for quality control in the traditional Chinese medicine production process.

The application discloses a characteristic spectrum construction method of medicinal leech and its close relative adulterants. The construction method comprises the following steps: a characteristic spectrum of medicinal leech and its close relative adulterants is established by high performance liquid chromatography, including extracting and preparing test sample solutions from medicinal leech and its close relative adulterants respectively; a control sample solution is prepared, and the test sample solution and the control sample solution are detected by high performance liquid chromatography, so that corresponding characteristic spectra are obtained, and medicinal leech and its close relative adulterants can be quickly distinguished. The medicinal leech control sample is selected from medicinal leech or leech of the Hirudinidae family; the close relative adulterants of the medicinal leech are selected from Limnatis humilis, Haemadipsa rills or Haemadipsa japonica. The method is simple and convenient, has the advantages of simple operation, good separation degree, high precision, good repeatability and good stability, and can realize rapid and accurate distinction of medicinal leech, leech and its close relative adulterants by HPLC.

This invention provides a method for determining the content of *Zanthoxylum bungeanum* seeds using HPLC, comprising: A) dissolving and extracting the sample raw material using a solvent to obtain a test solution; B) determining the test solution using high-performance liquid chromatography (HPLC) to obtain an HPLC chromatogram of *Zanthoxylum bungeanum* seeds; the HPLC conditions are as follows: a C18 column; mobile phase A is acetonitrile solution, mobile phase B is 0.1% phosphoric acid aqueous solution, and gradient elution is performed; specifically, the gradient elution is as follows: 0–20 min, phase A: 10% → 11%, phase B: 90% → 89%; 20–25 min, phase A: 11% → 18%, phase B: 89% → 82%; 25–40 min, phase A: 18% → 40%, phase B: 82% → 60%. This invention employs high-performance liquid chromatography (HPLC) with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. Using chlorogenic acid, neochlorogenic acid, and cryptochlorogenic acid as reference standards, an HPLC method for determining the content of *Zanthoxylum bungeanum* seeds was established, providing more scientific technical means for controlling the medicinal quality of *Zanthoxylum bungeanum* seeds.