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Method for preparing high-content phosphatidyl ethanolamine

A technology of phosphatidylethanolamine and cephalin, applied in the direction of edible phospholipid composition, food science, protein food ingredients, etc., can solve the problems of unfavorable popularization and application, long development cycle, expensive price, etc., to fill the domestic gap and reduce excessive dependence , The effect of easy production and operation

Active Publication Date: 2015-05-06
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Constrained by technology, domestic medicinal phosphatidylethanolamine mainly relies on imports at present, and the price is expensive and the delivery cycle is long, which leads to long development cycle and high cost of phosphatidylethanolamine-related products, which is not conducive to the popularization and application of the market

Method used

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  • Method for preparing high-content phosphatidyl ethanolamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Get 1 kilogram of soybean cephalin crude product (PE≥50%), add dichloromethane to dissolve and be made into raw material liquid, fill 10 kilograms of silica gels and carry out column chromatography, for the first time with eluent (dichloromethane 85.7%, ethanol 14.2 %, water 0.1%) to elute 18 liters, and then elute 45 liters with eluent (chloroform 73.5%, propanol 24.5, water 2.0%) for the second time to collect the eluate, concentrate in vacuo, and freeze-dry to obtain Phosphatidylethanolamine product, PE transfer rate is 85.4%, phosphatidylethanolamine content is 99.5%, phosphatidylcholine content is 0.3%, lysophosphatidylcholine content is not detected, acid value is 0.8, peroxide value is not detected The value of output and methoxyaniline is 3.6, and the content of triglyceride and free fatty acid all meet the regulations.

Embodiment 2

[0032] Get 1 kilogram of egg yolk cephalin crude product (PE≥50%), add chloroform to dissolve and be made into raw material liquid, fill 7 kilograms of diol-based silica gels and carry out column chromatography, first time with eluent (chloroform 90.0%) , propanol 9.8%, water 0.2%) to elute 15 liters, then elute 30 liters with eluent (dichloromethane 75.0%, propanol 24.5%, water 0.5%) for the second time to collect the eluate, and vacuum Concentrate and freeze-dry to obtain the phosphatidylethanolamine product, the PE transfer rate is 83.5%, the phosphatidylethanolamine content is 99.0%, the phosphatidylcholine content is 0.5%, the lysophosphatidylcholine content is not detected, and the acid value is 0.4, over The oxidation value was not detected, the methoxyaniline value was 1.0, and the contents of triglyceride and free fatty acid all met the regulations.

Embodiment 3

[0034] Get 10 kilograms of soybean cephalin crude product (PE≥50%), add dichloroethane to dissolve and be made into raw material liquid, fill 50 kilograms of diol-based silica gels and carry out column chromatography, use eluent (dichloroethane) for the first time 93.0%, ethanol 6.8%, water 0.2%) to elute 460 liters, then elute 440 liters with eluent (dichloroethane 80.0%, ethanol 19.0%, water 1.0%) to collect eluate for the second time, after Vacuum concentration, freeze-drying to obtain the phosphatidylethanolamine product, the PE transfer rate is 82.2%, the phosphatidylethanolamine content is 98.5%, the phosphatidylcholine content is 0.2%, the lysophosphatidylcholine content is not detected, and the acid value is 1.0, The peroxide value was not detected, the methoxyaniline value was 3.0, and the contents of triglyceride and free fatty acid all met the regulations.

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Abstract

The invention discloses a method for preparing high-content phosphatidyl ethanolamine. The method comprises the following steps of dissolving a cephalin crude product as a raw material with an organic solvent, eluting twice with a ternary mixed solvent comprising polyhalogenated alkanes, lower alcohol and water by column chromatography and drying to obtain the phosphatidyl ethanolamine product, wherein the obtained phosphatidyl ethanolamine product is high in content (96.0%-99.9% obtained by an HPLC method), low in content of impurities (in which the content of phosphatidylcholine is less than 0.5%, the content of lysophosphatidylcholine is less than 0.5 %, the content of free fatty acid is less than 0.2%, and the content of triglyceride is less than 0.2%), low in oxidation indexes (of which the acid number is less than 1.0, the peroxide value is less than 1.0 and the anisidine value is less than 5.0), and the product quality meets the quality requirements of drug phospholipid for injecting.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical engineering, and in particular relates to a preparation method of high-content phosphatidylethanolamine. Background technique [0002] Phosphatidylethanolamine (PE for short), also known as ethanolamine phosphoglyceride, is a class of phospholipid compounds composed of ethanolamine and phosphatidic acid in structure. It was used in the fields of food processing and health product research earlier. With the deepening of research, phosphatidylethanolamine has been gradually applied to the field of pharmaceutical preparations. In the most active field of liposome technology today, not only high-purity phosphatidylethanolamine Ethanolamine is an important source of excipients for liposome formulations and a key starting material for other important liposome excipients such as synthetic phospholipids (DOPE, DEPE, etc.). At present, the main control indicators of medicinal phospholipids recorded...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 许文东唐顺之李继荣袁诚关伟键蔡鸿飞杨玉琼谭巧君牟肖男梁北梅王小妹
Owner GUANGZHOU HANFANG PHARMA
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