Preparation method of bamboo leaf flavone

A technology of bamboo leaf flavonoids and extracts, which is applied in food preparation, medical preparations containing active ingredients, food science and other directions, can solve problems such as solvent loss, environmental pollution, poor selection of raw materials, etc., and achieve improved purity. Effect

Inactive Publication Date: 2009-03-25
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are many inventions and creations in the use of bamboo leaves in China. Patent No. 200310107871.5 is a Chinese patent on bamboo leaf antioxidants and their uses. See the composition of bamboo leaf antioxidants and their applications in some fields. The published application number is 98104564.2. Chinese invention patent---the production method of extracting flavonoids extract or powder from bamboo leaves provides a method for producing bamboo leaf flavonoids, but these technologies are still in the laboratory stage, and some technologies use a large amount of organic solvents. There are problems such as solvent loss and easy environmental pollution. The Chinese paten

Method used

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  • Preparation method of bamboo leaf flavone
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  • Preparation method of bamboo leaf flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1 HPLC method for screening high-content bamboo leaf total flavonoids method

[0086] (1) HPLC rutin standard solution: Accurately weigh 8.6 mg of rutin standard, dissolve in methanol in a 5 mL volumetric flask, and inject samples with different volumes. The standard equation is Y=746447X+262797.

[0087] (2) Orientin standard solution: Accurately weigh 3.3 mg of rutin standard, dissolve in methanol into a 5 mL volumetric flask, inject samples with different volumes, and the standard equation is Y=1155779X-47710.

[0088] (3) Vitexin standard solution: Accurately weigh 2.8 mg of rutin standard, dissolve in methanol in a 5 mL volumetric flask, and inject samples with different volumes. The standard equation is Y=1091100X+90365.

[0089] (4) 5% NaNO 2 Solution: Accurately weigh 2.5g NaNO 2 Solid, dissolved in distilled water and fixed volume in 50mL volumetric flask, set aside.

[0090] (5) 10%Al(NO 3 ) 3 Solution: Accurately weigh 5g Al(NO 3 ) 3 Solid, dis...

Embodiment 2

[0099] (1) Screening the lower part of Bamboo gooseberry in Nanjing area, drying the bamboo leaves at low temperature and avoiding light, and pulverizing, wherein the water content is 5.4%wt, and the content of flavonoids is 1.63%wt;

[0100] (2) Get 19 kg of pulverized bamboo leaves and extract them with 6 times the quality of industrial methanol; the extraction temperature is 50° C., the extraction time is 3 hours, and the extraction times are twice;

[0101] (3) Filtrate the leachate, concentrate in vacuum to 50 L, and the vacuum concentration temperature does not exceed 65° C. to obtain crude extract of bamboo leaves.

[0102] (4) Dilute the crude extract with water to 200L and filter to remove impurities

[0103] (5) After passing through a microporous membrane of 0.1 μm, the polysulfone membrane (PS) ultrafiltration membrane has a molecular weight cut-off of 4000; the nanofiltration membrane with a molecular weight cut-off of 400 is separated, and the material membrane f...

Embodiment 3

[0107] (1) Screening the lower part of the water bamboo in Nanjing area, drying the bamboo leaves at low temperature and avoiding light, and pulverizing them, wherein the water content is 5.5%wt, and the flavonoid content is 1.72%wt;

[0108] (2) Get 10 kg of pulverized bamboo leaves and extract with 80% ethanol with a mass concentration of 10 times the mass; the extraction temperature is 60° C., the extraction time is 10 h, and the extraction times are twice;

[0109] (3) Filtrate the leachate, concentrate in vacuum to 30 L, and the vacuum concentration temperature does not exceed 65° C. to obtain crude extract of bamboo leaves.

[0110] (4) Dilute the crude extract with water to 100L and filter to remove impurities

[0111] (5) Ultrafiltration through a 0.2 μm microporous membrane and a polysulfone membrane (PS) with a molecular weight cut-off of 10,000; separation by a nanofiltration membrane with a molecular weight cut-off of 650, and a material membrane flux of 8 m 3 / m ...

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Abstract

The invention discloses a preparing method for a bamboo leaves flavone extractive, which includes the following steps: breeds with high flavone content which are selected form 30 kinds of bamboo leaves can be taken as the production materials trough HPLC method; the selected bamboo leaves are dried at low temperature and lucifuge and smashed; and the C1-C3 is used for extracting; the lixivium is filtered and condensed in vacuum so as to obtain bamboo leaves gross extractive; and the gross extractive is purified through centrifugalizing, ceramic membrane, ultrafiltration membrane, nanofiltration membrane so as to obtain the bamboo leaves processed liquid; and the bamboo leaves processed liquid is separated and purified by certain size polymeric adsorbent resin, and is cooled to dry in vacuum or to dry with sprayer, so as to obtain the bamboo leaves flavone extractive with 40% to 65% of the flavone content.

Description

technical field [0001] The invention relates to the technical field of plant extracts, in particular to a method for extracting flavonoids from bamboo leaves and its preparation method, in particular to using bamboo leaves with high flavonoid content and wide distribution as raw materials, and ceramic membranes with specific apertures , ultrafiltration and nanofiltration of specific molecular cut-offs and special macroporous resins are well combined for purification. Background technique [0002] Bamboo is a class of plants belonging to the Gramineae Bambusadea (Bambusadea). There are about 70 genera and 1225 species in the world. Bamboo plants are naturally distributed in the tropical, subtropical and warm temperate regions of the earth between 46° north latitude and 47° south latitude, and are mainly distributed in tropical and subtropical regions of Asia, Africa, North and South America, Oceania and corresponding islands in the Pacific Ocean , with the tropical and subtr...

Claims

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Application Information

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IPC IPC(8): A61K36/899A23L1/30A61K127/00A23L33/105
Inventor 王成章陈文英叶建中陈虹霞周昊郑光耀
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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