104 results about "Pacific ocean" patented technology

The invention relates to a technology of absorbing fish by a cod end used for the trawl fishing of a large distant-water trawler, and belongs to the fields of farming, forestry, husbandry, and fishing. During a good harvest fishing time, large net heads of large trawlers used for catching scads in the south pacific and catching haddock in the north pacific can be frequently used for catching 500-600 tons fishing goods by a single net, and the weight of caught fishing goods by a single net sometimes can even reach up to 800 tons. Each large net head contains too many fishing goods, so that the fishes in each cod end are impossible to be directly pulled on a deck of the trawler. The technology of absorbing fish by cod end adopts an axial-flow type fish absorbing pump driven in a hydraumatic manner to directly absorb fish in the cod end into a fishhold of the trawler.

The invention discloses a screening method of Pacific oyster EST micro satellite label, which comprises the following steps: finding micro satellite site with Pacific oyster EST sequence announced by GeneBank and micro satellite searching software SSRhunter; screening and separating 2-6 base repeat unit micro satellite fragment (repeat times >5); getting repeat ESTs sequence with micro satellite; designing primer at two sides flank sequence of micro satellite repeat sequence; detecting optimization primer as the micro satellite label further; proceeding overall merit for micro satellite repeatability, stability, polymorphism; getting micro satellite label. The invention can be used for Pacific oyster species resource and genetic heterogeneity, molecular population genetics, species resource determination, genetic atlas construct, functional gene research, Pacific oyster breeding and cultivation.

The ocean salt monad of code of V3a and separated from deep sea bottom mud of west pacific ocean may be used in preparing biological polysaccharide through a simple fermenting culture process including providing common nutritious components, glucose, ammonium chloride, phosphate, magnesium sulfate, sodium chloride and 0.06 % concentration yeast paste as the growth factors, aerating and stirring at 18-28deg.c. The polysaccharide has low cost, simple extracting process, and wide application value. It has high flocculating activity, very high heat stability, obvious flocculating effect even on high temperature and acid or alkali conditions, effect of absorbing heavy metals, especially lead and wide application foreground in sewage treatment, food production and biochemical industry.

The invention discloses a prediction method for resource abundance of Japanese anchovies in the northwest Pacific Ocean. According to the method, the study sea region (from 32-degree N to 41-degree N and from 130-degree E to 145-degree E) is divided into 88 sea areas according to the spatial resolution of (1-degree x 1-degree), and an SST station is arranged in each sear area; SST data of each SST station is acquired through a remote sensing satellite; correlation analysis is performed on the SST data of each SST station in each month from April to September from 1988 to 2013 and CPUE data of Japanese anchovies in the next year, and the months when the SST stations are remarkably correlated with CPUE of Japanese anchovies in the next year are obtained; three linear regression models are established according to the correlation analysis result to predict the resource abundance of Japanese anchovies; and the three models are verified, the relative error of the second model is minimum according to a relative error, being the verification standard, between predicted resource abundance and actual resource abundance, and therefore the second model is selected to serve as the prediction method for the resource abundance of Japanese anchovies in the northwest Pacific Ocean.

The invention discloses a Pacific Ocean European flying squid resource complimentary quantity prediction method and application. The Pacific Ocean European flying squid resource complimentary quantity prediction method comprises steps of counting SST data of a sea area during a range of 125 degree-140 degree E, 25 degree to 40 degree N from 1997-2009, counting chl a concentration data of the sea area during a range of 125 degree-140 degree E, 25 degree-40 degree N from 1997 to 2009, counting CPUE of the Pacific Ocean European flying squid population from 1998 to 2010, counting an area of a sea area during a range of 125 degree-140 degree E, 25 degree-40 degree N from October to December, wherein the sea surface temperature in the sea area is from 15 DEG to 20 DEG and then calculating PS; obtaining a point of a time sequence of the CPUE, wherein the point has a highest correlation degree with the SST, chl a concentration data and the time sequence of the PS value; performing multi-linear regression analysis; using BP nerve network model prediction; and obtaining the CPUE prediction value of the Pacific Ocean European flying squid and calculating prediction accuracy.

The invention relates to an extension period forecasting method for northwest Pacific Ocean typhoon based on a statistical model. The method comprises the following steps: step 1) calculating the number of typhoons in the ten-day period of a historical stage; 2) extracting a secondary season component in the historical re-analysis field to obtain a secondary season sea-air environment field corresponding to the ten-day typhoon date; Large-scale fields at different advance times relative to typhoon generation are calculated respectively; 3) establishing a statistical forecasting model: performing correlation analysis on the large-scale fields at different advance times and the number of typhoons in the ten days of the historical stage, and establishing a statistical forecasting equation forforecasting the number of typhoons in the ten days of the historical stage; And 4) substituting a forecasting factor solved by the observed real-time large-scale field into the statistical forecasting equation to obtain the average frequency generated by the typhoon in the ten-day period and the occurrence probability of different generation numbers to form a forecasting quantity. The method hasthe advantages that a statistical forecasting model of northwest Pacific Ocean Typhoon elongation forecasting is established, and the number of generated Typhoons on northwest Pacific Ocean and the probability of the number are forecasted 2-3 weeks ahead of time.

The invention relates to a method for forecasting northwest Pacific Ocean squids resource stock recruitment. The method comprises the following steps of: 1, calculating the sea surface temperature data; 2, counting sea areas of which the sea surface temperature is 21-25 DEG C in the seas in the range of 130 DEG-170 DEG E and 20 DEG to 30 DEG N from January to April; 3, converting the sea surface temperature in the 130 DEG-170 DEG E and 20 DEG to 30 DEG N into a surface temperature contour map, and calculating the proportion of the seas of which the surface temperature is 21-25 DEG C (February AREASST, and the unit is %); 4, obtaining a formula of calculating squids resource abundance (CPUE, and the unit is t / d) on history; and 5, calculating the squids resource abundance CPUE=0.134449*AREASST-8.7821 in next year. The method has the advantages of: by the relational expression, more than 60% of the squids resource abundance change can be explained. The forecasting result of the northwest Pacific Ocean squids resource stock recruitment is provided for users and related departments in time and is taken as a scientific reference for fishery production and arranging; and the method can be released as a product in real time in future.

The invention discloses a northwest Pacific Ocean ommastrephidae bartramii winter-spring stock colony abundance prediction method based on a gray system. The northwest Pacific Ocean ommastrephidae bartramii winter-spring stock colony abundance prediction method comprises steps of (1) obtaining nino3.4 anomaly, PDO data, sea surface temperature SST and chlorophyll concentration ch1-a, and four sea environment and climate factors, (2) performing gray correlation analysis on four sea environment and weather factors, (3) choosing four factors having a highest association degree according to a gray association analysis result, wherein the four factors include: average sea surface temperature of an egg laying field in march, an interdecadal oscillation index of the pacific ocean in January, nino3.4 anomaly in march and average chlorophyll concentration in march; (4) establishing 8 prediction models based on the gray system according to four chosen factors; and (5) performing effective examination on 8 prediction models to choose a gray association model GM (1,4) structure as the prediction method for the northwest pacific ocean ommastrephidae bartramii winter-spring stock colony abundance. The prediction accuracy of the prediction method of the invention reaches more than 90%.

The invention discloses a northwest pacific three-dimensional oil spill business emergency forecasting and evaluating system. The system comprises an oil spill information rapid processing module, anenvironment information processing module, an oil spill transportation module, an oil spill weathering module, a data assimilation module, an ensemble forecasting module, a geographic information datamodule, a visual analysis module and a system control center module. According to the method, three-dimensional short-term and medium-and-long-term numerical simulation prediction of deep sea oil spill in the northwest Pacific Ocean sea area and application comparison of different vertical diffusion schemes can be realized under the consideration of the action of sea waves. Various kinds of oil source information and oil spill types can be preprocessed and predicted. An external forced field of the oil spill model can be optimized by utilizing field observation data and an optimal interpolation assimilation method in real time. Therefore, the forecasting accuracy is improved. An ensemble forecasting result can be provided. The system has important practical value for scientific research of marine ecological disaster prevention and reduction, design of an emergency response system of a management department and the like.

The invention relates to a method for indoor geoduck culture. By the method, the geoduck native to pacific coasts of Australia and America can be cultivated indoors at north coasts of China, and 3-5 years originally taken for geoduck individuals to grow to one pound in a sea area are shortened to less than 2 years taken for the geoduck individuals to grow to about 0.5kg. The method includes: burying geoduck seeds in substrate for dwelling, using inflating tubes to continuously fill air upwards so as to bring metabolites, residual bait, dirt and the like in the substrate to the surface of each cage, discharging the metabolites, residual bait and dirt with water flow. Therefore, the water environment for geoduck in the cages is constantly kept clean. By means of manual feeding, inflated pollution discharge, growth water temperature control, regular cage inverting for seed screening and the like, the geoduck individuals cultured indoors grow to 0.5kg in two years, and the yield per unit area reaches 10-12kg / m<2>.

The invention relates to a marine climate prediction method and system based on NPSDV driving factor identification. The prediction method comprises the following steps: acquiring sea surface salinitySSS analysis parameters, determining a time sequence of NPSDV and a driving factor index time sequence according to the SSS analysis parameters, calculating a time sequence power spectrum of the NPSDV, a driving factor index time sequence power spectrum, time sequence lag cross autocorrelation of the NPSDV, driving factor index time sequence lag cross autocorrelation and lag cross correlation, reconstructing spatial point SSS anomaly by using the autoregressive process model, determining an SSS anomaly reconstruction result, determining a driving factor according to the time sequence power spectrum of the NPSDV, the driving factor index time sequence power spectrum, the time sequence lag cross autocorrelation of the NPSDV, the driving factor index time sequence lag cross autocorrelation,the lag cross correlation and the SSS abnormal reconstruction result, and predicting the marine climate according to the driving factor. By adopting the prediction method and system provided by the invention, the marine climate can be accurately predicted.

The invention provides a method and system for analyzing ten group sources for an unknown-source individual. The method comprises the following steps: 1) extracting the DNA of the unknown-source individual; 2) obtaining the genotypes of 74 group specific sites of the DNA; and 3) obtaining the analysis results of the group genetic principal components of the genotypes of the specific sites by using STRUCTURE software, or obtaining the group result with the highest likelihood ratio from the genotypes of the specific sites by using FI software, wherein the ten groups include Sub-Saharan Africa, North Africa, Southwest Asia, Europe, South Asia, North Asia, East Asia, Southeast Asia, the Pacific and the America. The scheme provided by the invention can distinguish the groups in East Asia and also has relatively high resolution on other groups.

The invention particularly relates to a Schizophyllum strain and application thereof. The Schizophyllum strain is collected by General Microorganism Center of China Committee for Culture Collection of Microorganisms on November 24th, 2015, the collection number is CGMCC No.11604, and the collection name is Schizophyllum 15R-5-F01. The Schizophyllum strain provided by the invention is taken from the lignite bed from a 1923-deep marine bottom sediment in Pacific Ocean and prepared by carrying out separation and purification, and is a new strain after being identified. The comprehensive morphological identification and ribosome RNA (ribonucleic acid) sequential analysis results indicate that the strain 15R-5-F01 is a Schizophyllum strain, and belongs to Schizophyllum, Agaricales and Basidiomycota. The Schizophyllum strain provided by the invention has very obvious coal powder adsorption effect, and can be used for producing methane by using lignite as a raw material.

One kind uses the Pacific Ocean Alaska pollack fish flesh preparation protein widowed peptide powder the medium technique, its operation technology method is: After the Pacific Ocean Alaska pollack fish flesh will twist garrulously, will increase the 20AU / kg~~80AU / kg bacillus subtilus inscribe proteinase and the 8000LAPU / kg~~40000LAPU / kg tune mold circumscribe proteinase; Or increases the 20AU / kg~80AU / kg bud spore bacillus to conform to the proteinase, with 1.2: 1~1: 1.5 water even mixes, in the enzyme reactor enzymolysis 2hr~12hr, enzymolysis temperature 50deg.C~60deg.C, outset PH is equal to 6.6, the enzymolysis fluid passes through deactivation, the centrifugal separation, the membrane separation, again spray drying or drying. Then obtains the water-soluble protein widowed peptide powder and the wastage. The invention preparation protein widowed peptide powder is the pasteurized milk white, does not have the bitter taste, the micro fish fishy smell, the molecular weight (is bigger than 85%) to be possible separately to control for is smaller than was equal to 1000Da and 1000~5000Da, the protein widowed peptide powder returns-ratio (relative raw material total protein) achieves 65%~79%.

The invention relates to a method for calculating high-precision Chinese offshore sea wave characteristic distribution. According to the method, a meteorological model WRF, a large-area sea wave modelwavewatch 3 and a sea wave model swan used in the offshore area of China are combined, and a multi-year historical sea wave characteristic data product with the spatial resolution of 0.04 degree * 0.04 degree can be calculated. As the China offshore area is divided into the East China sea area and the South China sea area by considering the monsoon influence area and the wave propagation characteristics during model establishment. The western pacific ocean is prevented from being brought into a mode range, and computing resources are prevented from being consumed. Due to the fact that the twosea areas have the overlapping area, the overlapping area has two calculation results, a weighted average method is used, the calculation results of the two overlapping areas are integrated, and thewhole set of data has spatial continuity.

A method for forecasting the central fishery of squid in the North Pacific Ocean, including time-space scale setting, environmental factor setting, and establishment of a central fishery prediction model. The factor setting uses surface temperature (SST) as the main environmental factor, supplemented by two environmental factors, sea surface height (SSH) and chlorophyll a (Chl-a), and the environmental factors are divided into four situations when establishing the central fishery prediction model: According to the setting of time-space scale and environmental factors, a sample plan set of 24 situations is established; the central fishery prediction model adopts the classic error backpropagation BP neural network model, and the BP neural network model has a three-layer structure, namely the input layer and the hidden layer And the output layer, the input layer inputs the spatio-temporal factors and environmental factors of the fishery, and the output layer outputs CPUE or the fishery level index converted from CPUE for forecasting.

The invention discloses an automatic identifying system of northwest Pacific Ocean annular tropical cyclone. Firstly, an automatic identifying system specific to the northwest Pacific Ocean annular tropical cyclone is built up; typhoon satellite data, environment field, sea temperature field data to be judged are input to the automatic identifying system; the system can automatically identify a series of physical features of the typhoon on the basis of these feature parameters through pre-screening and linear judgment or BP neutral network, and warn the typhoon in accordance with the feature of the northwest Pacific Ocean annular tropical cyclone, thereby providing help for forecasting this kind of typhoon.

The invention discloses microsatellite primers for identifying crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas and a hybrid thereof and an identification method.The microsatellite primers are F:5'-CGACTGGTGGGAGTTTCTGAC-3' and R:5'-GCCGCTTCTATCTCCTTTGC-3'.Due to the fact that the external morphology of oysters changes greatly along with different living environments, identification is usually difficult only according to morphological characteristics; the oysters in the larval stage are more difficult to distinguish according to the morphological characteristics.By means of the microsatellite primers and the identification method, crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas individuals can be identified rapidly and accurately, and identification of the hybrid individual of the crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas individuals has the potential application value.Meanwhile, the microsatellite primers for identifying the crassostrea hongkongensis, the crassostrea ariakensis and the crassostrea gigas and the hybrid thereof and the identification method have the advantages of being simple in method, visual, accurate and effective in result, free of influences of environment and growth stage, low in cost and the like.

The invention discloses a method to estimate the intensity of tropical cyclones across the Northwest Pacific Ocean by using a geostationary meteorological satellite image. The method is characterized by comprising the following steps: extracting the information of the infrared window area brightness temperature and the water vapor channel brightness temperature of a typhoon inner core area in a satellite image in which the interval of longitude is equal to the interval of latitude ; acquiring the WIRA index values by using the brightness temperature information; calculating the mean value for total WIRA index values acquired, to acquire the mean value , and to count total corresponding pixel points W when the WIRA index values are in the range of in the typhoon core area; and using the typhoon intensity equation: I=985.474-0.033xW-2.391x10<-5>xW<2> to obtain the typhoon intensity. This method can indicate the symmetric level changes for the convective axis, and can also exclude thin cirrus, stratospheric water vapor anomalies and penetrating convection, and can provide indexes that are best related with the intensity of the optimal path of a typhoon, so that the typhoon intensity is calculated more accurately.

Automated in-situ instrumentation has been developed for sensitive, precise and accurate measurements of a variety of analytes in natural waters. In this work we describe the use of ‘SEAS’ (Spectrophotometric Elemental Analysis System) instrumentation for measurements of solution pH. SEAS-pH incorporates a CCD-based spectrophotometer, an incandescent light source, and dual pumps for mixing natural water samples with a sulfonephthalein indicator. The SEAS-pH optical cell consists of either a liquid core waveguide (LCW, Teflon AF 2400) or custom-made PEEK tubing. Long optical pathlengths allow use of indicators at low concentrations, thereby precluding indicator-induced pH perturbations. Laboratory experiments show that pH measurements obtained using LCW and PEEK optical cells are indistinguishable from measurements obtained using conventional spectrophotometric cells and high-performance spectrophotometers. Deployments in the Equatorial Pacific and the Gulf of Mexico demonstrate that the SEAS-pH instrument is capable of obtaining vertical pH profiles with high spatial resolution. SEAS-pH deployments at a fixed river-site (Hillsborough River, Fla.) demonstrate the capability of SEAS for observations of diel pH cycles with high temporal resolution. The in-situ precision of SEAS-pH is better than 0.002 pH units, and the system's measurement frequency is approximately 0.5 Hz. This work indicates that in-situ instrumentation can be used to provide unique capabilities for observations of carbon-system transformations in the natural environment.

The invention discloses a method applied to identification of pacific oyster family and belongs to the technical field of genomic molecular markers. The method comprises the following steps: (1) searching type I and type II SNP mutation sites in an SNP database acquired by an Illumina GoldenGate method; (2) establishing genotype database files of filial generations and parents, and acquiring genetic parameters of each SNP site by utilizing Cervus 3.0 software; (3) performing family simulation identification and analysis according to the allele frequency of each site by utilizing the Cervus 3.0 software, performing actual family identification and analysis, calculating a LOD value of a candidate parental pair, and selecting parents with the highest LOD value being positive value as real parents. According to the invention, an SNP marking method applied to identification of pacific oyster family is developed, and the reaction system and polymerase chain reaction (PCR) amplification conditions are optimized. The experiments prove that the primers have the characteristics of high repeatability, high stability, high solubility curve resolution and the like, and a foundation is laid for breeding of the pacific oyster family.

The embodiment of the invention provides a satellite short-data communication method, a satellite short-data communication device and a satellite short-data communication system between a ship and a shore. The system comprises a ship satellite terminal, a satellite space segment, a ground station, a land terminal and the satellite short-data communication device, wherein the satellite space segment comprises a maritime satellite covering the Indian Ocean region and the Pacific Ocean region, and a maritime satellite covering the Atlantic Ocean region; the ground station comprises a first maritime satellite ground station and a second maritime satellite ground station, wherein the first maritime satellite ground station is communicated with the maritime satellite covering the Indian Ocean region and the Pacific Ocean region and the second maritime satellite ground station is communicated with the maritime satellite covering the Atlantic Ocean region; and the satellite short-data communication device is connected with the first ground station, the second ground station and a land user terminal, receives short messages sent by the land terminal, and selects to send the short messages to the first ground station or the second ground station according to the sate of the ship satellite terminal, or sends the short messages received from the first ground station or the second ground station to the land terminal. The system realizes short-message communication between a global sailing ship and the land user terminal.

The invention relates to a method for authenticating oyster population, in particular to a novel method for authenticating Pacific oyster population, comprising the steps of sample collection, genome extraction, primer design, PCR amplification, and EST-SSRs specific site authentication. In the step of sample collection, sample individuals from different populations of ostrea cucullata, Pacific oyster and crassostrea rivularis are collected; in the step of genome extraction, genomes of a plurality of samples of each oyster population are extracted; in the step of primer design, EST-SSRs primers are designed according to an expressed sequence tag (EST) sequence containing microsatellites; in the step of PCR amplification, the EST-SSRs primers are used to carry out the PCR amplification to obtain the PCR product with a target segment; and in the step of EST-SSRs specific site authentication, agarose gel electrophoresis is firstly carried out on the PCR product to obtain the PCR product with clear and stable bands, and then polyacrylamide gelelectrophoresis is carried out to detect polymorphic sites so as to authenticate the allele Pacific oyster population having the allele sites with specific EST microsatellite sequence. In the invention, an EST-SSRs molecule marker technology is utilized, the primers are reasonably designed, and two-step electrophoresis detections are adopted so as to achieve the purpose of authenticating the Pacific oyster population. The invention has the advantages of simple method, time saving and accurate analysis.

The invention relates to a method for predicting access fishing of the western and central pacific ocean katsuwonuspelamis purse seine fishery. The method adopts statistic data of each year before a prediction year to first 16 years, which comprises a year, a month, a longitude, a latitude, a fishing effort (shooting times) and a fish catch (ton) in a region from 15 degrees south to 15 degrees north and from 125 degrees east to 180 degrees east, SSTA (Sea Surface Temperature Anomaly) in a Nino3.4 region and an SST (Sea Surface Temperature) in operation waters. The method is characterized in that a 5 degrees * 5 degrees spatial resolution region is used as one sea area, production statistic data of the sea areas in 16 years are matched with corresponding environment data one by one to obtain a relationship of different SSTA and SST ranges and corresponding initialized fishing efforts in each sea area, and an access fishing prediction model of each sea area is established by utilizing a normal distribution model; the basis is provided for scientific access fishing of the western and central pacific ocean katsuwonuspelamis purse seine fishery; blindness of fishing is greatly reduced; and fishing efficiency is improved.

A prediction method for North Pacific Ocean squid resource replenishment quantity is characterized by comprising following steps: selecting marine environment factors of sea water having high correlation as correlative factors of foraging habitat environment influencing squid resource replenishment quantity by means of the correlation between the time series formed by sea environment factors in a foraging field in foraging months and a CPUE time series of this year; selecting marine environment factors of sea water having high correlation as correlative factors of spawning habitat environment influencing resource replenishment quantity by means of the correlation between the time series formed by sea environment factors in a spawning field in spawning months and a CPUE time series of the next year; Using the ratio of the suitable surface water temperature range to the total area in the spawning months of spawning fields and the ratio of the suitable surface water temperature range to the total area in the foraging area, the habitat environment suitable degree of the spawning fields and the foraging fields are expressed by PS and PF; Using selected environment factors and different combinations of PS and PF, establishing a BP network configuration prediction model and selecting the optimal models for medium and long term fishery condition prediction.

The invention provides a pair of primers which can be named as E-EEL170 used for discriminating anguilla marmorata and anguilla bicolor pacifica fry by using microsatellite markers, and a sequence of the primer is shown as SEQ ID No.1 and 2. While discriminating, a common DNA extraction kit is used for extracting DNA of 2 types of eel fry genome and performing PCR amplification on DNA of 2 types of eel DNA samples by the primer pair, 1% of sepharose gel (EB dyeing) electrophoresis is used for detecting the PCR products, strips with 250bp size are anguilla marmorata eel fry, and strips with 500bp size are anguilla bicolor pacifica eel fry. The primer is capable of discriminating anguilla marmorata and anguilla bicolor pacifica fry by taking a few of tail fin without execution of fry, and has the advantages of high stability, good repeatability, strong practicality and easy operation, and can rapidly and accurately discriminate the anguilla marmorata and anguilla bicolor pacifica fry.

The invention provides a parent crassostrea nippona breeding method for promoting maturity and spawning. The method comprises the steps of firstly, selecting and placing parent crassostrea nippona in a nursery pond for maturity-promoting breeding; feeding the parent crassostrea nippona with crassostrea nippona monodus subterraneus bait, and conducting maturity-promoting breeding on the crassostrea nippona by raising the temperature step by step, so that gonad of the parent crassostrea nippona achieves maturity; drying the parent crassostrea nippona achieving gonad maturity in the shade, and then placing the parent crassostrea nippona into a spawning pond; picking out female mature pacific oysters through microscopic examination, taking out ova through dissection, filtering the ova with a screen, and removing tissue fragments and pacific oyster ova, so that tissue filtered fluid is obtained; adding the tissue filtered fluid into the spawning pond, and inducing the parent crassostrea nippona to spawn and discharge seminal fluid; finally, moving away the parent crassostrea nippona, so that fertilized ova of artificial breeding are obtained. By means of the method, the problems that it is difficult for parent crassostrea nippona bred through indoor artificial maturation-promoting breeding to get mature and spawn can be solved, the parent crassostrea nippona spawns quickly, spawn quality is good, the artificial breeding production requirements for the crassostrea nippona are satisifed, and the foundation is laid for large-scale production of the crassostrea nippona.

The invention discloses a kit for detecting nervous necrosis viruses of pacific codfish. The kit comprises an upstream primer and a downstream primer for a PCR reaction and is characterized in that the upstream primer sequence is shown as SEQ ID NO.1; the downstream primer sequence is shown as SEQ ID NO.2; and the plasmid standard substance of a nervous necrosis virus gene contains the sequence shown as SEQ ID NO.3. The kit for detecting the nervous necrosis viruses of the pacific codfish, disclosed by the invention, has the advantages of strong specificity, high sensitivity, good repeatability, wide detection linear range and the like. The kit disclosed by the invention can be widely applied to the artificial culture of the pacific codfish; and parent fish carrying no virus is selected to breed and sperms, eggs, fertilized eggs and fish larvae are monitored in real time to guarantee that virus amount carried in the parent fish, the sperms, the eggs, the fertilized eggs and the fish larvae is at a low level and avoid that lots of fries are dead due to virus outbreak, so that the bottleneck of the artificial breeding of the pacific codfish is overcome.

The invention provides a method for extracting a quasi-biweekly oscillation real-time index based on an EEOF. The method comprises the steps of (1) performing EEOF analysis on an OLR anomaly field ofbandpass filtering in set time periods of -a, -b and 0 day delays in a data sequence of a monsoon region to obtain two EEOF modes, wherein a is larger than b, and both a and b are positive integers, (2) projecting an OLR anomaly field of no bandpass filtering in set time periods including -a, -b and 0 days to the two above EEOF modes to obtain a projection result of each day, and standardizing theprojection result of each with a standard deviation in a period of time in the data sequence to obtain the quasi-biweekly oscillation real-time index. The method for extracting a quasi-biweekly oscillation real-time index has the advantages that the spread characteristics of QBWO convection current in the northwest and north directions on the northwestern Pacific Ocean and the Indian Ocean can beeffectively captured.

The invention discloses an ENSO (El Nino-Southern Oscillation) aggregative indicator and a runoff prediction method thereof. Duration time (MEImonth) is used for reflecting the persistence of an El Nino / La Nina event; average intensity (MEImean) and extreme intensity (MEImax) are used for expressing the intensity characteristics of the El Nino / La Nina event; a range that the temperature increase of equatorial pacific sea temperature is greater than or equal to 0.5DEG C(or less than or equal to 0.5) is used for representing the space expansibility (MEIspa) of a warm water region; a region of which the temperature change achieves or exceeds + / -2DEG C represents the expansion range (MEIexspa) of an extreme warming area; and the five parameters are used for constructing the ENSO aggregative indicator (MEIZ). The method comprehensively inspects the influence of the El Nino / La Nina event on atmosphere, the function of the method is that every event is scientifically distinguished, and the influence degree of the El Nino / La Nina event on the atmosphere is objectively evaluated.