One kind uses the Pacific Ocean Alaska pollack fish flesh preparation protein widowed peptide powder the medium technique, its operation technology method is: After the Pacific Ocean Alaska pollack fish flesh will twist garrulously, will increase the 20AU/kg~~80AU/kg bacillus subtilus inscribe proteinase and the 8000LAPU/kg~~40000LAPU/kg tune mold circumscribe proteinase; Or increases the 20AU/kg~80AU/kg bud spore bacillus to conform to the proteinase, with 1.2: 1~1: 1.5 water even mixes, in the enzyme reactor enzymolysis 2hr~12hr, enzymolysis temperature 50deg.C~60deg.C, outset PH is equal to 6.6, the enzymolysis fluid passes through deactivation, the centrifugal separation, the membrane separation, again spray drying or drying. Then obtains the water-soluble protein widowed peptide powder and the wastage. The invention preparation protein widowed peptide powder is the pasteurized milk white, does not have the bitter taste, the micro fish fishy smell, the molecular weight (is bigger than 85%) to be possible separately to control for is smaller than was equal to 1000Da and 1000~5000Da, the protein widowed peptide powder returns-ratio (relative raw material total protein) achieves 65%~79%.