49 results about "Orientin" patented technology

A method for extracting and purifying a flavonoid glycoside monomer from a sensitive plant belongs to the technical field of Chinese traditional medicine. The technical scheme is as following: taking the sensitive plant as a raw material; performing ultrasonic extraction and concentration, and then performing alcohol precipitation to remove impurities and reconcentration; separating and enriching by macroporous resin column chromatography, and then separating by combing a gel resin column chromatography technology and a silica gel column chromatography technology to obtain three monomeric compounds including vitexin, orientin and isoorientin, wherein, the three monomeric compounds have the extraction yield of 1.12%, 2.62% and 2.21% respectively, and the purity of the three is above 95%. The method has the advantages of convenient operation, low cost, high purity, high yield, repetitive recycle of the macroporous resin and the solvent, disuse of toxic solvents in the process, no environmental pollution, easy industrialized production and the like.

The invention discloses choline eutectic solvents, a preparation method and an application in extraction of flavone C-glycoside of flos trollii. The choline eutectic solvents have the expression: [N(CH)33CH2CH2OH]+[X.zY]- and is prepared from choline bromide or choline chloride and ZnCl2, SnCl2 or AlCl3 through mixing in a certain mole ratio, wherein when X is Br, Y is ZnCl2 or SnCl2; when X is Cl, Y is AlCl3. By means of the provided choline eutectic solvents, three important flavone C-glycosides, namely, orientin, orientin-2''-O-beta-L-galactoside and vitexin in the flos trollii can be efficiently and selectively extracted; compared with conventional extraction with methanol solvents, the extraction rate of orientin, orientin-2''-O-beta-L-galactoside and vitexin can be improved, few impurities exist in an extract, silica gel columns are not required to be repeatedly used for separation when the extract serving as the raw material is used for preparing monomers of orientin, orientin-2''-O-beta-L-galactoside and vitexin, the separation and purification steps can be greatly reduced, and the separation and purification cost can be reduced.

The invention provides a method for providing active compound orientin with myocardium nourishing function from bamboo leaf. The method is characterized in that bamboo leaf raw material is smashed, the smashed bamboo leaf is added into 8-12 times alkaline water containing borax, heating extraction is carried out for 2-3 times, extracting solution is acidized and then is added into a polyamide chromatographic column, ethanol-water mixed solvent is used for elution, eluent is concentrated, butyl alcohol is used for extraction for 2-3 times, an ODS silicagel column is used for separation, methyl alcohol-water-acetic acid mixed solvent is used for pressurization and elution, thin-layer chromatography monitoring is carried out, orientin fraction is collected, and vacuum drying is carried out, thus obtaining the product. The method has simple operation, high product content and good repeatability.

The invention provides novel flavone hydroxylase, a microorganism for synthesizing a flavone C-glycoside compound and an application of the novel flavone hydroxylase. The inventor clones to obtain novel flavone hydroxylase PhF2H and PhF3'H, and the novel flavone hydroxylase PhF2H and PhF3'H belongs to cytochrome P450 hydroxylase and has a function of hydroxylating a specific position of the compound. Furthermore, the inventor efficiently synthesizes the flavone C-glycoside compound, such as orientin, isoorientin, vitexin and isovitexin, and related intermediates such as eriodictyol, 2-hydroxynaringenin and the like in a synthetic route of the flavone C-glycoside compound in an artificial recombinant expression system by modifying the enzyme and combining with the assembly of a synthetic route of C-glycoside glycosyltransferase and a flavone precursor.

The present invention provides a cannabis-based flavonoid pharmaceutical composition including any one or more selected from the group of Apigenin, Cannflavin A, Cannflavin B, Cannflavin C, Chrysoeriol, Cosmosiin, Flavocannabiside, Kaempferol, Luteolin, Myricetin, Orientin, Isoorientin (Homoorientin), Quercetin, (+)-Taxifolin, Vitexin, and Isovitexin, or their synthases, for the prevention and treatment of certain diseases of the CNS system and related disorders.

The invention discloses a C-glycosylflavones composition. The composition is extracted from plants of genus Trollius, and mainly contains orientin, vitexin, or vitexin esters and orientin esters. The invention also discloses a preparation method of the C-glycosylflavones composition. The preparation method is characterized by comprising extracting Trollius Chinensis Bge., performing macroporous resin column chromatography, and performing selective gradient elution with ethanol-water mixed solvent. The macroporous resin is preferably weak polarity macroporous resin. The C-glycosylflavones composition has pharmacological effects in resisting oxidation and inflammation, and inhibiting bacterial infection; and can be applied as ingredient of drugs and functional foods.

A quality control method for the compound Hongcao medicine in the form of freeze-dried powder injection, injection, or orally taken medicine features that an efficient liquid-phase chromatography is used to measure the contents of scutelloside, isoorientin and orientin.

The invention discloses novel application of composition with the content of flavone C-glycoside 2'-O-beta-L-galactopyranosyl orientin and 2'-O-beta-L-galactopyranosyl orientin between 30 and 90 percent in preparing medicaments for resisting angiocardiopathy such as myocardial ischemia and the like, and also describes a single component of the 2'-O-beta-L-galactopyranosyl orientin, a method for preparing the composition, and a medicinal preparation.

The invention relates to a natural pharmaceutical composition for preventing or treating cardiovascular and cerebrovascular diseases, in particular relates to a pharmaceutical composition consisting of ginkgolide and natural C-glycosyl flavone compounds, and especially relates to a medicine which takes ginkgolide B and vitexin or orientin as major active ingredients. Experimental researches show that the composition has a significant effect on improving and treating diseases of cardiovascular and cerebrovascular system. The pharmaceutical composition disclosed by the invention is wide in source of the adopted raw materials, natural and non-toxic, low in side effect and relatively low in cost, and the pharmaceutical composition has a broad value of practical application.

The invention relates to the technical field of extraction and application of total flavonoids of plants and relates to extract of total flavonoids of cyperus esculentus, an active monomer orientin and an extraction method and application thereof. The preparation method of the extract of total flavonoids of cyperus esculentus comprises carrying out reflux extraction on aerial stems and leaves of cyperus esculentus through an ethanol solution, mixing the extract, carrying out reduced pressure concentration to obtain extract, carrying out macroporous resin column chromatography on the pretreatedextract, carrying out elution in separation orderly through water and an ethanol solution with the content of 30% to obtain eluate of the ethanol solution with the content of 30%, and orderly carrying out reduced pressure concentration and freeze drying through the eluate of the ethanol solution with the content of 30% to obtain the extract of total flavonoids of cyperus esculentus. The inventiondiscloses the application of the extract of total flavonoids of cyperus esculentus and the active monomer orientin in preparation of drugs for treating ischemic stroke. The pharmacological experimentresult shows that the extract of total flavonoids of cyperus esculentus and the active monomer orientin can protect I/R injury.

The invention, which belongs to the technical field of food quality control, specifically provides a method for measuring contents of three kinds of components in the aspalathus linearis (brum.f.) r.dahlgren, the aspalathus linearis (brum.f.) r.dahlgren extractive, and the aspalathus linearis (brum.f.) r.dahlgren tea. The method comprises: step one, preparing a mixed reference solution; step two,preparing a test sample solution; step three, carrying out chromatographic condition and system adaptability testing; to be specific, carrying out measurement based on the high performance liquid chromatography under the conditions of using the octadecylsilane chemically bonded silica column as a column, acetonitrile as a mobile phase A, an acetic acid solution with the volume concentration of 0.8to 1. 5% or formic acid with the volume concentration of 0.1 to 0.5% as mobile phase B; carrying out chromatographic analysis according to a gradient elution procedure under conditions of controllingthe flow rate of the mobile phase of 0.8 to 1.2 ml/min and the detection wavelengths of 275 to 295nm and 340 to 360nm; and step four, performing a measuring method; to be specific, abstracting the mixed reference solution and the test sample solution respectively and injecting the solutions into a liquid chromatograph and measuring and calculating contents of isoorientin, orientin, and aspalathine. The invention provides a method for measuring contents of three kinds of components in the aspalathus linearis (brum.f.) r.dahlgren, the aspalathus linearis (brum.f.) r.dahlgren extractive, and theaspalathus linearis (brum.f.) r.dahlgren tea rapidly, accurately, and conveniently.

The invention provides a new application of Orientin in medicine, specifically the application of Orientin in the preparation of medicine for treating acute gout. Experimental research results show that Orientin has a protective effect on the acute gout model of human vascular endothelial cell damage caused by urate, and inhibits the expression of ICAM‑1. Therefore, Orientin can be used to prepare a drug for treating acute gout inflammation. The use of Orientin involved in the present invention in the preparation of medicines for the treatment of acute gout is disclosed for the first time. Since the skeleton type belongs to a new skeleton type, and its preventive and treatment activity for acute gout is so strong that it is unexpected, there is no possibility of giving any inspiration from other compounds , has outstanding substantive features, and it is obviously a significant progress in the prevention and treatment of acute gout.

The invention discloses a composite using orientin-2'-O-beta-L-galactoside as a main component, which is prepared by performing extraction on dried flowers of trollius chinensis bunge, trollius ledebouri reichb and trollius macropetalus fr. schmidt. Pharmacological experiments show that the composite has the effects of preventing and treating prostatitis, prostatic hyperplasia and the like. The composite has new application in the preparation of medicaments for preventing and treating prostatic diseases. The medicinal preparation of the composite may be any one of tablets, capsules, pills, grains, suspension, dropping pills, paste, suppository rectal administration preparation and the like.

The invention provides a method for determining the content of active ingredients in Chinese honeylocust fruit, which adopts ultra-high performance liquid chromatography-mass spectrometry. The content of L-malic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, scopoletin, liquiritigenin, apigenin, luteolin, eriodictyol, citrus aurantium, fustin, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, vitexin, isovitexin, quercitrin, orientin and isoorientin in the Chinese honeylocust fruit can be rapidly detected. The method provided by the invention has the advantages of simple operation, high sensitivity, fast analysis speed and strong specificity, and can be used for quality control of Chinese honeylocust fruit.

The invention belongs to the technical field of plant extraction and medicine, and provides a fernleaf hedge bamboo extract, and a preparation method and application thereof. The preparation method ofthe fernleaf hedge bamboo extract comprises the following steps: with fernleaf hedge bamboo leaves as a raw material, extracting the fernleaf hedge bamboo leaves to obtain a fernleaf hedge bamboo leaf extract; adding acid into the fernleaf hedge bamboo leaf extract to enable the final concentration of hydrogen ions in the fernleaf hedge bamboo leaf extract to reach 0.25-2 mol/L, and then carryingout an acid hydrolysis reaction to obtain a hydrolysate; and carrying out extraction separation on the hydrolysate to obtain the fernleaf hedge bamboo extract. According to the method, hydrochloric acid or sulfuric acid is added into the fernleaf hedge bamboo leaf extract for acid hydrolysis reaction, so the content of other bamboo leaf flavonoids and other components in the fernleaf hedge bambooextract can be reduced, the content of isoorientin and vitexin in the fernleaf hedge bamboo extract can be increased, and convenience is brought to purification of isoorientin and vitexin monomers.

The invention belongs to the technical field of cosmetics, and provides a preparation method and application of a trollius chinensis bunge extract. The preparation method comprises the following steps: crushing trollius chinensis bunge, mixing the crushed trollius chinensis bunge with a solvent to obtain a mixture, and extracting the mixture by using a micro-jet extractor to obtain the trollius chinensis bunge extract. By extraction through the micro-jet extractor, cells in trollius chinensis bunge raw materials can be broken effectively, effective components are dissolved out, chemical reagents do not need to be added or a complicated technological process does not need to be adopted, effective components in traditional Chinese medicine components can be retained, meanwhile, harmful components are prevented from being remained, and the prepared trollius chinensis bunge extract is high in content of orientin and apigenin-8-C-glucoside and low in irritation, and can be used for preparing antioxidant or anti-aging cosmetics.

The invention discloses applications of flavone C-glycoside monomer compounds vitexin, isovitexin, orientin and isoorientin in preparation of analgesic drugs and drugs for promoting wound healing, wherein the analgesic activity of the isoorientin is remarkably higher than the analgesic activity of aspirin and rotundine, and orientin and the vitexin have strong effect of wound healing promoting.

The invention provides a morinda citrifolia enzyme collagen peptide compound beverage and a preparation method thereof, and the morinda citrifolia enzyme collagen peptide compound beverage comprises the following raw materials in parts by weight: 30-95 parts of morinda citrifolia enzyme stock solution, 10-50 parts of collagen peptide, 1-30 parts of inulin, 1-5 parts of haematococcus pluvialis, 1-5 parts of vitamin C, 1-5 parts of [gamma]-aminobutyric acid, 2-8 parts of phosphatidylserine, 1-5 parts of nicotinic acid, 1-5 parts of vitamin E, 2-3 parts of buckwheat flavone, 1-3 parts of naringenin and 3-7 parts of orientin. The morinda citrifolia enzyme stock solution and the collagen peptide are effectively combined, the anti-aging, anti-oxidation and anti-saccharification effects are enhanced, the bitter and fishy smell of morinda citrifolia enzyme or collagen is improved through technological treatment, the content of various nutrient elements in the beverage is effectively reserved, and the content of polypeptide and protein in the beverage is high.

The invention discloses an application of a flavone C-glycoside monomer in food preservation; and the flavone C-glycoside compound is selected from orientin, isoorientin, vitexin or isovitexin.