15 results about "Orientin" patented technology

The invention relates to the technical field of plant processing, in particular to a preparation method of a typical bamboo leaf C-glycoside flavone magnetic molecularly imprinted polymer. The preparation method comprises the following steps: designing bamboo leaf carbon glycoside flavone as a template molecule, and preparing a magnetic molecularly imprinted nano polymer MIP with a core-shell structure by adopting a surface molecular imprinting technology; the characterization of TEM, XRD, VSM, FT-IR and the like proves that the prepared MIP is successfully synthesized, and the satisfactory adsorption capacity (for template molecules of 16.10 mg / g) is obtained. The MIP is added into the bamboo leaf flavonoid crude extract, and the total flavonoid content obtained after adsorption is increased from 24% to 90% or above. Six flavonoid compounds are detected from the high-purity flavonoids through HPLC (High Performance Liquid Chromatography), and four C-glycoside flavonoids account for 90.73%; the content of the rubisoside and the isorubisoside is 33-40%, the content of the vitexin and the isovitexin is 40-48%, and the value and the biological activity of the bamboo leaf flavonoid extract are remarkably increased.

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a process optimization method for green extraction of hemsley rockvine root flavonoid glycoside through ultrasonic cooperation with a surfactant. The method comprises the following steps: optimizing process parameters for extracting isoorientin, isoquercitrin, kaempferol-3-O-rutinoside and astragalin from radix tetrastigme tuberous roots by using an ultrasonic-assisted lauryl sodium sulfate aqueous solution micelle medium; influences of the type and concentration of a surfactant, a material-liquid ratio, ultrasonic power, ultrasonic time, ultrasonic times and ultrasonic temperature on target components are investigated, and optimal extraction process parameters are determined: the concentration of sodium dodecyl sulfate (SDS) is 35mg / mL, the ultrasonic power is 100W, and the ultrasonic temperature is 50 DEG C. According to the process, a traditional organic solvent is replaced by the surfactant, so that the extraction efficiency is improved; a feasible scheme is provided for green extraction of active ingredients of radix tetrastigme, and industrial amplification feasibility can be further explored subsequently.

The invention discloses a method for preparing a high-purity C-glycoside flavone monomer by using secondary reversed-phase semi-preparative chromatography, which comprises the following steps of: performing primary elution on bamboo leaf flavone by using a C18 chromatographic column under the conditions of set column temperature, mobile phase system and elution mode, and monitoring and collecting a primary purified product of a peak corresponding to a target component; carrying out reduced pressure concentration on products of isoorientin and orientin corresponding peaks in the first purification product to remove the solvent, redissolving residues by using an organic solvent II, carrying out second elution on a sample solution obtained by filtering through a microfiltration membrane by using a C18 chromatographic column in a set column temperature, a mobile phase system and an elution mode, and collecting eluent; monitoring and collecting a secondary purified product of a peak corresponding to the target component, and performing vacuum concentration and freeze drying. The bamboo leaf flavonoid product obtained by the method has the purity of not less than 98%, the raw material utilization rate is obviously improved, the separation efficiency is high, and the purification period is greatly shortened.

The application discloses a flavone 6-C-glycosyltransferase mutant for improving the yield of isohyduloside, and provides a mutant protein of GyCGT1, which comprises the following steps: mutating the amino acid residues at positions 7, 8, 9, 18, 205, 258, 280 and 417 of SEQ ID NO: 2, and keeping the amino acid residues at other positions unchanged, so that a protein with the same function is obtained; or the mutant protein of GyCGT1 comprises the following steps: mutating the amino acid residues at positions 7, 9 and 205 of SEQ ID NO: 2, and keeping the amino acid residues at other positions unchanged, so that a protein with the same function is obtained. The glycosyltransferase GyCGT1 mutant is obtained through the method, and the yield of isohyduloside is increased by 8 times in the fermentation of the constructed Escherichia coli recombinant strain in a 50mL shaking flask system, so that the mutant has a good prospect for industrial production application.

The invention relates to the technical field of bamboo leaf detection, and discloses a bamboo leaf detection method based on thin-layer chromatography, which comprises the following steps: S1, preparation of a reference substance solution: respectively taking vitexin, isoorientin and orientin, and adding methanol for dissolving to prepare a mixed solution as a mixed reference substance solution; s2, preparation of a test solution: taking a to-be-detected sample, adding an extraction solvent for extraction, taking supernate, and filtering to obtain the test solution; s3, thin layer chromatography determination: taking the reference substance solution and the test solution, respectively dispensing the reference substance solution and the test solution on the same polyamide film, adding a developing solvent, developing, taking out, airing, spraying a color developing agent, drying until spots are clearly developed, and then placing under an ultraviolet lamp for inspection. According to the method, the bamboo leaves can be rapidly and effectively identified by displaying spots with the same color at the corresponding positions of the reference substance chromatogram, and the method is simple and convenient to operate, high in precision and sensitivity and good in stability, can more accurately identify the bamboo leaves compared with the prior art, and has a good application prospect.

The invention discloses a lophatherum gracile and sorghum sweet wine production process based on a subcritical technology. The lophatherum gracile and sorghum sweet wine production process comprises the following steps: mixing cleaned pest-free sorghum and lophatherum gracile with water; putting the mixture into a subcritical kettle, and treating by adopting a gradient heating / variable pressure process, so as to improve the dissolution rate of flavone and effectively degrade tannin; cooling the heated mixture to 30-40 DEG C, and inoculating sweet wine koji for fermentation; after fermentation, filtering the fermented mash to obtain fermentation liquor; and heating and sterilizing the fermentation liquor, and cooling to obtain the lophatherum gracile and sorghum sweet wine. According to the process, the raw material soaking and cooking time in a traditional sweet wine brewing process can be shortened, and the production efficiency of the lophatherum gracile and sorghum sweet wine is improved; meanwhile, tannin substances in sorghum are degraded by adopting a subcritical technology, and the obtained lophatherum gracile and sorghum sweet wine is free of bitter taste; thirdly, dissolution of flavonoid compounds such as isovitexin and isoorientin in lophatherum gracile is accelerated by adopting a subcritical technology, and independent extraction is not needed.

The application discloses a method for extracting and separating isoheteroside from Gastrodia elata Bl. The method uses a surfactant aqueous solution as an extraction medium and combines ultrasonic technology to replace an organic solution in a conventional extraction process. Through orthogonal experiment design, main process factors influencing the extraction rate are optimized to determine a relatively optimal process parameter combination. On the basis, isoheteroside is separated and prepared by using a strong ion exchange column gradient elution. The process condition of the application is mild, the extraction time is relatively short, the method has good reproducibility, and the application provides a feasible technical reference for large-scale production of isoheteroside and total flavones.

The invention discloses a duckweed LtF3 'H gene and application thereof. Relates to the technical field of gene engineering. The invention provides a gene sequence, an amplification primer and application. The key function of the F3 'H gene in plant secondary metabolites is that F3' H can catalyze naringenin to generate eriodictyol, and eriodictyol can influence accumulation of anthocyanidin, so that the content of cyanidin-3-o-glucoside in a duckweed LtF3 'H overexpression strain is remarkably increased, meanwhile, F3' H can catalyze apigenin to synthesize luteolin, and the yield of luteolin is increased. The luteolin is a mother nucleus of orientin and isoorientin, so that the contents of orientin and isoorientin in an overexpression strain of the duckweed LtF3 'H are remarkably increased, and meanwhile, the content of apigenin C-glycoside (vitexin and isovitexin) is reduced.

The invention discloses a flavone 6-C-glycosyl transferase mutant capable of increasing the yield of isoorientin and a preparation method of the flavone 6-C-glycosyl transferase mutant. The mutant protein of GyCGT1 provided by the invention is a protein with the same function obtained by mutating amino acid residues at the 7th site, the 8th site, the 9th site, the 18th site, the 205th site, the 258th site, the 280th site and the 417th site of SEQ ID NO: 2 and keeping the amino acid residues at other positions unchanged; or the mutant protein of the GyCGT1 is obtained by mutating amino acid residues at the 7th site, the 9th site and the 205th site of SEQ ID NO: 2, and the amino acid residues at other positions are not changed, so that the protein with the same function is obtained. The glycosyl transferase GyCGT1 mutant is obtained through transformation, an escherichia coli recombinant strain is constructed for fermentation, the yield of isoorientin in a 50mL shake flask system is increased by 8 times, and the glycosyl transferase GyCGT1 mutant has a good prospect in industrial production application.

The invention discloses application of two small molecular substances in regulation and control of weed suppression activity of flavonoid glycoside substances. The invention provides any one of the following applications of a GST activator and a GST inhibitor: (A1) regulating the inhibition activity of wheat flavone-5-O-glucoside to weeds; and (A2) the inhibition activity of the isoorientin on weeds is regulated. According to the invention, the weed suppression effect of the flavonoid glycoside substances can be obviously improved, and the use of chemical pesticides is reduced, so that a novel biological control method is provided for agricultural production, and green agriculture and sustainable development can be realized.

The invention belongs to the technical field of medicines, and particularly relates to application of orientin in preparation of a medicine for treating breast cancer. An MMTV-PyMT breast cancer transgenic mouse is adopted, it is found that the tumor weight inhibition rate of 31.2% is achieved when the PD-L1 monoclonal antibody is independently used, the inhibition rate of orientin single drug treatment is 25.6%, after the PD-L1 monoclonal antibody and the orientin single drug are jointly used, tumor growth can be remarkably inhibited, the tumor inhibition rate reaches 70.0%, and the combined treatment effect is remarkably superior to simple superposition of the drug effects of all the single drugs. In addition, the combined treatment can also effectively promote the infiltration of CD4 + and CD8 + T cells in tumor tissues, and improve the expression level of key anti-tumor immune factors such as granzyme B (GZMB) and interferon-gamma (IFN-gamma).

The invention discloses a preparation and quality detection method of a Pimpinella diversifolia fruit standard decoction, and particularly provides a detection method of the Pimpinella diversifolia fruit standard decoction, which comprises the following steps: detecting the Pimpinella diversifolia fruit standard decoction by high performance liquid chromatography; the pimpinella diversifolia fruit standard decoction is prepared from chlorogenic acid, isoorientin, isovitexin, luteolin and apigenin. Chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is an octadecyl bonded silica gel chromatographic column; the mobile phase A is acetonitrile, and the mobile phase B is 0.05-0.1% acetic acid aqueous solution; and gradient elution. The method is good in stability, high in precision and good in reproducibility. The fingerprint spectrum of the standard decoction of the pimpinella diversifolia fruit decoction pieces has seven common peaks, five components are determined through identification and are all main effective components of the pimpinella diversifolia fruit, a basis can be provided for establishment and quality control of the fingerprint spectrum of the formula granules, and the clinical effectiveness of the pimpinella diversifolia fruit formula granules is ensured.

The application discloses a construction method of a snake medicinal liquor HPLC fingerprint and a component content determination method thereof, and belongs to the technical field of traditional Chinese medicine preparation detection and analysis. The construction method of the snake medicinal liquor HPLC fingerprint comprises the following steps: (1) preparation of a test sample solution; (2) preparation of a mixed control sample solution; and (3) establishment of a fingerprint. The determination method of the multi-component content of the snake medicinal liquor comprises the following steps: (1) preparation of a test sample solution; (2) preparation of a mixed control sample solution; (3) calculation of a relative correction factor; and (4) determination of the content of a to-be-detected component. The HPLC fingerprint of the snake medicinal liquor is established, and the content of isohispidin, hispidin and luteolin in the snake medicinal liquor is determined by combining with the one-measurement-multiple-evaluation method, the information characteristics of the whole components of the snake medicinal liquor can be comprehensively reflected, the detection operation is quick, the cost is low, the efficiency is high, and the scientific and reasonable basis can be provided for the overall quality evaluation and standard improvement of the snake medicinal liquor.

The present application relates to a kind of methods for constructing transgenic tobacco plants capable of heterologous synthesis flavonoid carbon glycoside substance and its application, belong to genetic engineering technical field.The present application provides EbCHS Gene, EbCHI Gene, PhF2H Gene, PhF3' H Gene and ZmUGT708A6 The application of gene in heterologous synthesis flavonoid carbon glycoside.The present application scheme is by chemical synthesis EbCHS 、 EbCHI , PhF2H 、 PhF3' H And ZmUGT708A6 Flavonoid carbon glycoside synthesis related gene, constructs multi-gene expression vector, is successfully obtained by agrobacterium-mediated transformation transgenic tobacco capable of expressing multiple genes, by determination, the transgenic tobacco can successfully synthesize four kinds of flavonoid carbon glycoside such as vitexin, isovitexin, sophorin and isosophorin, provides technical support for natural medicine efficient preparation and medicinal plant germplasm innovation.

The application provides application of flupirtine and analogues thereof in preparation of a medicament for treating pigmented skin diseases. The analogues of the flupirtine include voruciclib, ostruthin or vitexin. The experimental results of the application show that after melanocytes and melanoma cells are treated by FH and analogues thereof, the melanin production capacity of the cells is significantly reduced, including inhibition of cell proliferation, reduction of melanin content, significant down-regulation of expression levels of key genes MITF, TYR, TYRP1 and the like for melanin production, and reduction of tyrosinase activity. Further in a zebrafish embryo model, we observe strong whitening efficacy and safety of FH. The research results suggest that FH and analogues thereof are expected to become a candidate therapeutic drug for pigmented skin diseases.