70 results about "Isovitexin" patented technology

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The invention discloses a folium microcotis total flavone extract, and a preparation method and an application thereof. The extract contains 50 wt% to 95wt% of general flavone based on rutin, 3.5 wt% to 7.5 wt% of narcissoside in the total flavone extract, 0.5 wt% to 3.0 wt% of vitexin and 0.5 wt% to 3.0 wt% of isovitexin. The preparation method comprises the steps as follows: coarse extracting; taking one or more of water, methanol and ethanol as an extraction solvent for coarse extracting; refining: taking a macroporous resin or a polyamide or a silica gel as an adsorbent, ethanol or other organic solvents as an eluent for eluting, collecting the eluent, and decompressing and concentrating to obtain the folium microcotis total flavone extract. The folium microcotis total flavone extract provided by the invention contains 50 wt% to 95 wt% of the general flavone based on the rutin, and has the obvious effect of adjusting blood fat. The preparation method is simple and effective, thereby being suitable for industrial mass production and capable of preparing medicines for adjusting the blood fat.

The invention discloses a method for preparing a flavonoid glycoside and stibene glucoside type compound by separating from fenugreek. By virtue of a high-speed countercurrent chromatography (HSCCC) separation technology, isomerides such as vitexin and isovitexin and stibene glucoside type compounds such as rhaponticin, deoxyrhapontin and rhapontigenin which have the similar polarity to that of the isomerides in the fenugreek are subjected to separation preparation, so that the separation time is effectively shortened, and the shortcomings of complicated operation, sample die-adsorption loss, low yield and the like of the conventional preparation method are overcome. According to the method, the technology is simple, the reproducibility is high, and the separation efficiency is high; the obtained monomer content is higher than 96.0 percent and can meet the requirements of a type of botanical drugs and standard contrasts; and the method is suitable for industrial production of medical medicines, standard substances, health protection food, health protection medicines and the like.

This invention is to provide multiple specific inhibitors of cytochrome P450 isozyme CYP2C9. These inhibitors can be derived from any combinations with the following compounds including: Tamarixetin, Formononetin, isoliquritigenin, Phloretin, luteolin, Quercitrin, quercetin, myricetin, Wongonin, Puerarin, Genistein, Nordihydroguaiaretic acid, Narigenin, Capillarisin, Chrysin, Fisefin, eriodictyol, 6-Gingerol, Isorhamneti, isoquercitrin, Morin, (+)-Taxifolin, isovitexin, 3-Phenylpropyl Acetate, Oleanolic acid, ursolic acid, β-Myrcene, cinnamic acid, Luteolin-7-Glucoside, Liquiritin, (+)Limonene, Homoorientin, Swertiamarin, Embelin, Daidzein, Poncirin, (−)-Epicatechin, ergosterol. These natural products can be used to enhance the bioavailability of therapeutic agents (drugs).

The invention discloses a method for extracting and separating viterxin and isovitexin from a natural product. The method comprises the following steps: based on santalum album L leaves as raw materials, carrying out ultrasonic extraction and lixiviation on the santalum album L leaves by utilizing an alcohol solvent; then respectively extracting santalum album L leaf extract by adopting petroleum ether, chloroform and ethyl acetate in turn; carrying out column chromatography on ethyl acetate extract, and recrystallizing the obtained eluent so as to obtain the isovitexin; and separating a residual liquid through a preparation liquid phase so as to obtain the viterxin. In the method, the santalum album L leaves are used for the first time so as to extract out the viterxin and isovitexin, the separation process is simple, the cost is low, and the raw materials are available; the santalum album L leaves contain high viterxin and isovitexin contents, thus a novel way for resource utilization of santalum album L leaves is provided; the two compounds can be prepared into small hydro-acupuncture drop injections, freeze-dried powder, tablets, capsules or pills; and the viterxin can be used for preparation of medicaments for treating coronary disease, myocardial infarction, angina and cancer prevention tumour resistance, and the isovitexin can be used for preparation of medicaments for treating or preventing prostate cancer, breast cancer and endometrial cancer.

The invention relates to the extraction technology of active ingredients of cajan leaf which is a Chinese herbal medicine, and aims at providing a high-efficiency enzyme inductive extraction method which can economically and quickly obtain a cajan leaf extract rich in five flavonoid active ingredients, namely agnuside, isovitexin, luteolin, apigenin and isorhamnetin. The method is directed against the shortcomings of the traditional extraction method such as long extraction time, high extraction temperature, low extraction efficiency and the like and adopts technologies such as homogenate fragmentation enzymolysis, homothermal enzymolysis, vacuum cavitation extraction, solvent decontamination and enrichment and the like to obtain the cajan leaf extract rich in five flavonoid active ingredients; and the result shows that: the content of the five flavonoid active ingredients contained in the extract can respectively reach 0.223 to 0.296, 0.145 to 0.187, 0.194 to 0.264, 0.108 to 0.150 and 0.074 to 0.102 mg / g, which is 9 percent to 65 percent higher than the content of the five flavonoid active ingredients contained in the extract obtained by the traditional hot reflux method. The method is characterized by high extraction ratio, simple equipment and safe operation, needs no high-temperature heating, is applicable to industrial production and provides a new method and means for the modernization of Chinese traditional medical extraction.

The invention discloses common lophatherum herb extract and a preparation method and application thereof. The common lophatherum herb extract comprises isovitexin, swertiajaponin, swertisin, luteolin 7-O-beta-D-glucose-6-C-alpha-L-arabinoside, orientin and vitexin. The preparation method for the common lophatherum herb extract comprises the following steps of: extracting the dry medicinal material of common lophatherum herb by using ethanol aqueous solution to obtain crude extract, and purifying by using a macroporous adsorption resin column. The extract can be used for preparing a medicine or health care product for resisting respiratory syncytial virus, parainfluenza virus type 3 or influenza A virus.

The present invention relates to a method for extracting and purifying functional components of Chinese herbal medicine. The purpose is to provide a simple, safe, economical and efficient method for extracting and purifying monomeric compounds vitexin and isovitexin from pigeonpea branches and leaves. The technical scheme is: using fresh or dried branches and leaves of pigeonpea as raw materials, using homogenate extraction technology, ultrasonic assisted extraction technology, ultrasonic oscillation flocculation technology, negative pressure cavitation suspension liquid extraction technology, macroporous adsorption resin enrichment technology, ODS -C18 reverse phase medium pressure silica gel column chromatography technology combined with low temperature crystallization and recrystallization technology to obtain vitexin and isovitexin with a purity of over 90%, and the extraction rates are over 0.17% and 0.13% respectively . The raw materials used in the invention are fresh or dried pigeonpea branches and leaves which are mostly discarded in agricultural production, which neither destroys the ecological balance of plants nor affects agricultural production, and can obtain high-purity pigeonpea functional components. Moreover, the method is simple and easy to implement after extraction, preliminary impurity removal and repurification, and the loss of the target compound is small. It is suitable for industrial applications and is of great significance to industrial production.

The invention provides health radix tetrastigme tea and a preparation method thereof. The health radix tetrastigme tea comprises the following raw materials in parts by weight: 60-80 parts of radix tetrastigme leaves and radix tetrastigme cirrus in all, and 20-40 parts of tubers and roots of the radix tetrastigme. The preparation method comprises the following steps: dewatering and drying all raw materials in a constant-temperature drying oven at 50-80 DEG C; and carrying out superfine grinding on the dried materials so as to prepare ultramicro powder. According to the health radix tetrastigme tea provided by the invention, leaves of the radix tetrastigme are utilized; and the tubers and roots of the radix tetrastigme can be effectively utilized, so that the radix tetrastigme plant is free of a leaking part; and the plant is effectively utilized. According to the preparation method of the health radix tetrastigme tea provided by the invention, drying is carried out at 50-80 DEG C, so that the damage to effective components such as rhamnosylvitexin, isovitexin rhamnoside, isovitexin and vitexin in the raw materials caused by high temperature is avoided; and the effective components are reserved to the maximal extent.

The invention provides novel flavone hydroxylase, a microorganism for synthesizing a flavone C-glycoside compound and an application of the novel flavone hydroxylase. The inventor clones to obtain novel flavone hydroxylase PhF2H and PhF3'H, and the novel flavone hydroxylase PhF2H and PhF3'H belongs to cytochrome P450 hydroxylase and has a function of hydroxylating a specific position of the compound. Furthermore, the inventor efficiently synthesizes the flavone C-glycoside compound, such as orientin, isoorientin, vitexin and isovitexin, and related intermediates such as eriodictyol, 2-hydroxynaringenin and the like in a synthetic route of the flavone C-glycoside compound in an artificial recombinant expression system by modifying the enzyme and combining with the assembly of a synthetic route of C-glycoside glycosyltransferase and a flavone precursor.

The present invention relates to a method for preparing high-purity isovitexin from the herbal medicinal herb by using semi-preparative high-performance liquid chromatography. Ethyl ester and n-butanol were extracted until colorless, evaporated to dryness, dissolved in 50% methanol, and separated by reversed-phase semi-preparative high-efficiency liquid phase under certain chromatographic conditions. Fractions with the same retention time were collected and combined, and concentrated under reduced pressure. The isovitexin monomer with a purity of more than 98% can be obtained. The present invention uses only one chromatographic system to perform isocratic elution, can quickly separate high-purity isovitexin, produces less waste in the inventive method, occupies a small area, and has high column efficiency, and the method is simple and easy .

The present invention provides a cannabis-based flavonoid pharmaceutical composition including any one or more selected from the group of Apigenin, Cannflavin A, Cannflavin B, Cannflavin C, Chrysoeriol, Cosmosiin, Flavocannabiside, Kaempferol, Luteolin, Myricetin, Orientin, Isoorientin (Homoorientin), Quercetin, (+)-Taxifolin, Vitexin, and Isovitexin, or their synthases, for the prevention and treatment of certain diseases of the CNS system and related disorders.

The invention relates to a gentiana macrophylla capsule fingerprint spectrum and an application of the spectrum to quality control and component analysis. According to the spectrum, a HPLC (high-performance liquid chromatography) fingerprint spectrum common mode of a gentiana macrophylla capsule is determined by the aid of a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system (2004A), 23 common peaks are calibrated, similarity is 0.966-0.982, and the content of loganic acid, shanzhiside methyl ester, 6'-O-beta-D-glucose gentiopicroside, swertiamarin,gentiopicroside, sweroside, isoorientin and isovitexin in 10 preparations is measured. According to the spectrum, a HPLC fingerprint spectrum method of the gentiana macrophylla capsule and a content measuring method are built, the method has good analysis evaluation ability and the advantages of accuracy, convenience, stability, reliability and the like, so that the method can serve as an effective evaluation method for the quality of the preparation.

The invention relates to an anti-inflammatory effective part of a leaf of Santalum album L.. The anti-inflammatory effective part comprises vitexin, isovitexin and orientin, and the content of the vitexin, the isovitexin and the orientin in the effective part is not less than 80%. The invention further provides a preparation method and application of the anti-inflammatory effective part of the leaf of Santalum album L.. The anti-inflammatory effective part of the leaf of Santalum album L. is clear in chemical components and the content of the known components exceeds 80%; systematic anti-inflammatory experiments show that the effective part is effective in treatment of acute inflammation.

The invention provides a method for extraction and separation of isovitexin. The method comprises the following steps of subjecting Desmodium styracifolium to reflux extraction by utilizing ethyl alcohol so as to obtain a Desmodium styracifolium extract; and subjecting the Desmodium styracifolium extract to multistage chromatographic separation by utilizing an alcohol system, wherein the step of multistage chromatographic separation comprises the following sub-steps of subjecting the Desmodium styracifolium extract to a first chromatographic separation by utilizing a first alcohol system so as to obtain a first separation part, subjecting the first separation part to a second chromatographic separation by utilizing a second alcohol system so as to obtain a second separation part, and subjecting the second separation part to a third chromatographic separation by utilizing a third alcohol system so as to obtain the isovitexin. The invention also provides a system for implementing the above-mentioned method. By utilizing the method and / or the system for extraction and separation of the isovitexin from the Desmodium styracifolium, process operation is simple; production time is short; product yield is high; purity is high; and the isovitexin with a purity reaching to 99.7% can be obtained.

The invention provides a microcos paniculata flavone carbon glycoside compound and a preparation method and application thereof. The preparation method of the microcos paniculata flavone carbon glycoside compound comprises the steps that macroporous resin adsorbs a water extract of the microcos paniculata, after elution is conducted sequentially through water and 10% ethyl alcohol, 70% ethyl alcohol elution fraction is collected, the fraction is subjected to acid hydrolysis and neutralization to obtain a transition product; and the transition product is eluted by using 80% methyl alcohol, andthe microcos paniculata flavone carbon glycoside compound is obtained through separation and purification. The microcos paniculatat flavone carbon glycoside compound is prepared from the following components of vitexin, isovitexin, apigenin-6,8-C-diglucoside, apigenin-6-C-arabinose-8-C-glucoside, apigenin-6-C-glucose-8-C-arab glycoside, apigenin-6-C-xylose-8-C-glucoside, apigenin-6-C-glucose-8-C-rhamnoside, and apigenin-6-C-rhamnose-8-C-glucoside, wherein the content of apigenin carbon glycoside compounds accounts for 50% or more of the mass of the flavone carbon glycoside compound. The microcos paniculata flavone carbon glycoside compound can effectively improve acute lung injury of a model mouse caused by LPS, and has relative good application prospects on development of drugs for treating acute lung injury and lung inflammation.

The invention relates to the technical field of traditional Chinese medicinal material extraction technologies and medicines, in particular to a preparation method of high-activity oldenlandia diffusatotal flavonoid, namely the oldenlandia diffusa total flavonoid is extracted by directly adopting a small amount of cellulase. Compared with other extraction methods, the method disclosed by the invention is simple to operate, low in energy consumption and higher in feasibility, and the extracted total flavonoids (higher content of isoflavone components) are higher in biological activity; the method for preparing the oldenlandia diffusa total flavonoid can be used for obtaining three components which are not extracted by other extraction methods, namely dihydrokaempferol, apigenin and chrysin; six components, namely naringin, vitexin, isovitexin, genistein, luteolin and 7-O-glucoside luteolin, of which the contents are remarkably increased (up-regulated by two times or more) are obtained;the nine compounds have various biological activities such as inflammation resistance, oxidation resistance, tumor resistance, liver protection and the like; and the high-activity oldenlandia diffusatotal flavonoid extract prepared by the invention can be used for preventing and treating liver peroxidation injury and can play a role in assisting in reducing blood fat.

The invention relates to a carbon glycosyl transferase DhCGT2 gene in a desmodium heterocarpum plant and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the carbon glycosyl transferase DhCGT2 gene in the desmodium heterocarpum plant is as shown in SEQ ID NO.1, and the total length of the sequence is 1422 bp; and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, 473 amino acid residues are encoded, and the encoded protein can be used for preparing schaftoside and isoschaftoside. The carbon glycosyl transferase is found in desmodium plant desmodium heterocarpum for the first time, and has the functions of catalyzing the generation of vitexin and isovitexin by taking dyhydroxy naringenin as a substrate and catalyzing the generation of schaftoside and isoschaftoside by taking dyhydroxy naringenin glucose C-glycoside as a substrate; and an efficient biosynthesis method for heterologous expression of vitexin, isovitexin, schaftoside and isoschaftoside in vitro of plants is provided.

The invention relates to a method for extracting isovitexin from ficus microcarpa, and particularly relates to a method for extracting the isovitexin by combining an application flash extraction technology and enzymolysis-ultrasonic-assisted extraction. The method comprises the following treatment steps: placing fresh or dry ficus microcarpa into a container, adding 70%-80% ethanol, and carrying out smashing extraction on the ficus microcarpa on a traditional Chinese medicine extractor for 5-10 minutes; taking out, regulating a pH value to 4.0-5.5, and carrying out enzymolysis and ultrasonic-assisted extraction; filtering, alkalifying filter liquor for neutralization, purifying by adopting macroporous adsorption resin, and recrystallizing to obtain the high-purity isovitexin. The method disclosed by the invention is simple in process, has complementary advantages by combining two technologies, greatly shortens the extraction time and is low in cost, more favorable to environmental protection and suitable for industrialized production.

The invention belongs to and relates to a novel gentiana capsule optimized medicinal composition as well as application thereof in analgesia, anti-inflammatory and cholagogic aspects. The invention provides a gentiana capsule optimized medicinal composition; the gentiana capsule optimized medicinal composition is prepared from the following effective components: 120 to 150 parts of loganic acid, 5to 10 parts of shanzhiside methyl ester, 40 to 45 parts of 6'-O-beta-D-glucosyl gentiopicrin, 10 to 15 parts of swertiamarine, 500 to 520 parts of gentiopicrin, 8 to 10 parts of sweroside, 1 to 2 parts of isoorientin and 1 to 2 parts of isovitexin. The medicinal composition has the advantages that the activity of the medicinal composition in the analgesia, anti-inflammatory and cholagogic aspectsis obviously superior to that of the gentiopicrin, the gentiana capsule and gentian total glycoside extract at the aboveground part of the gentian.

The invention discloses a folium microcotis total flavone extract, and a preparation method and an application thereof. The extract contains 50 wt% to 95wt% of general flavone based on rutin, 3.5 wt% to 7.5 wt% of narcissoside in the total flavone extract, 0.5 wt% to 3.0 wt% of vitexin and 0.5 wt% to 3.0 wt% of isovitexin. The preparation method comprises the steps as follows: coarse extracting; taking one or more of water, methanol and ethanol as an extraction solvent for coarse extracting; refining: taking a macroporous resin or a polyamide or a silica gel as an adsorbent, ethanol or other organic solvents as an eluent for eluting, collecting the eluent, and decompressing and concentrating to obtain the folium microcotis total flavone extract. The folium microcotis total flavone extract provided by the invention contains 50 wt% to 95 wt% of the general flavone based on the rutin, and has the obvious effect of adjusting blood fat. The preparation method is simple and effective, thereby being suitable for industrial mass production and capable of preparing medicines for adjusting the blood fat.

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin,β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The invention provides a method for determining the content of active ingredients in Chinese honeylocust fruit, which adopts ultra-high performance liquid chromatography-mass spectrometry. The content of L-malic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, scopoletin, liquiritigenin, apigenin, luteolin, eriodictyol, citrus aurantium, fustin, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, vitexin, isovitexin, quercitrin, orientin and isoorientin in the Chinese honeylocust fruit can be rapidly detected. The method provided by the invention has the advantages of simple operation, high sensitivity, fast analysis speed and strong specificity, and can be used for quality control of Chinese honeylocust fruit.

The invention discloses a method for quickly measuring the content of flavonoid constituents in paniculata. The method is used for measuring the contents of epicatechin, vitexin, isovitexin and narcissoside in paniculata, wherein the contents can be quickly measured by using an HPLC (High Performance Liquid Chromatography) method. The method can be used for measuring the contents of the four flavonoid constituents, namely the epicatechin, the vitexin, the isovitexin and the narcissosid, in the paniculata simultaneously through one step. The measurement method provided by the invention has the advantages of high speed, simplicity, accuracy, high sensitivity and good stability, and is very beneficial to the application in quick quality control of medicinal materials of the paniculata in a drug preparation process.

The invention provides a method for extracting high-purity vitexin and isovitexin from santati album leaves. The method comprises the steps: repeatedly extracting dried and smashed santati album leaves through ethyl alcohol or methyl alcohol for twice to three times, combining filtrates, decolorizing through a decolorizing agent, performing vacuum concentration and sequentially utilizing petroleumether and ethyl acetate to extract concentrate for twice to three times; combining ethyl acetate extract liquor, concentrating, freeze drying, fully dissolving in water bath and centrifuging to divide into precipitate and supernatant; centrifuging the precipitate in 75 to 85 DEG C water bath and freeze drying the obtained precipitate to obtain the vitexin; concentrating the supernatant and utilizing a cooling crystallization mode to obtain isovitexin. The purity of the vitexin and the isovitexin prepared by the method is higher than 98%; furthermore, the method has the advantages of being simple and convenience in operation, low cost and high extracting efficiency; the obtained product has a high purity, and large-scale production is achieved.

The invention discloses a detecting method for creeping oxalis. The method comprises an identifying method and a content measuring method for isovitexin. The identifying method and the content measuring method for the isovitexin of the detecting method are accurate in result, high in sensitivity, good in repeatability and reliable in result, the quality of the creeping oxalis can be controlled effectively, and the medical effect of the creeping oxalis is ensured.

The invention discloses a preparation method and application of a flavonoid glycoside monomer isovitexin of Hydrocotyle sibthorpioides. The method comprises the following steps: 1) adding Hydrocotyle sibthorpioides into an ethanol solution, performing ultrasonic extraction, then performing digestion with the ethanol solution multiple times, and performing vacuum concentration until ethanol is completely volatilized to obtain a crude ethanol extract; 2) sequentially extracting the crude ethanol extract with petroleum ether, chloroform and ethyl acetate, and successively conducting concentrating and drying to obtain an ethyl acetate extract; and 3) carrying out silica gel column chromatographic separation on the ethyl acetate extract, carrying out gradient elution with ethyl acetate, methanol and formic acid successively, collecting eluate, and performing recrystallizing with methanol to obtain a product. According to the application, the isovitexin is used for preparing a medicine used for preventing and treating the non-alcoholic fatty liver disease, and the protection mechanism of the isovitexin is inflammation prevention, anti-oxidative stress, lipid peroxidation and down-regulation of endoplasmic reticulum stress related protein expression. The invention provides a novel compound with a clear structure and high efficiency for the treatment of the non-alcoholic fatty liver disease, and develops a new medication approach.

The invention relates to an extraction and purification method of the component with the Chinese herbal medicine functionality, and aims at providing a method of obtaining monomeric compound Vitexin and Isovitexin through extraction and purification from pigeon pea branches and leaves in a simple, safe, economical and high-efficient manner. The invention has the technical proposal of using the fresh and dried pigeon pea branches and the leaves as raw materials, and adopting the combination of a homogenate extraction technique, an ultrasonic assistance extraction technique, an ultrasonic oscillation flocculation technique, a negative pressure cavitation suspension extraction technique, a macropore absorbing resin collecting technique, an ODS-C18 reversed phase medium pressing silicagel column chromatography technique, a low temperature recrystallization and a recrystallization technique, and then the Vitexin and the Isovitexin with the purity of more than 90 percent are obtained, and the extraction rate is more than 0.17 percent and 0.13 percent respectively. The raw material used by the invention is the wasted fresh or dried pigeon pea branches and leaves in the agricultural production, thus the invention cannot damage the ecological balance of plants and affect the agricultural production, and can further get the pigeon pea functionality component. Moreover, the method has the steps of extraction, primary decontaminating and purification, and is simple and practical with little objective compound, thus the method is applicable for the industry application, and has important meaning to the industrialized production.

The invention discloses a method for simultaneously measuring the content of six ingredients in canarium pimela leenh. leaves. The method comprises the following steps: 1, preparation of a sample solvent: drying and crushing the canarium pimela leenh. leaves, performing ultrasonic extraction on the crushed canarium pimela leenh. leaves, and performing volume fixation and filtering to obtain the sample solvent; 2, preparation of a reference solvent, wherein the reference solvent is prepared from known amounts of chlorogenic acid, vitexin, isovitexin, hyperoside, rutin and quercetin; 3, chromatographic analysis: performing high performance liquid chromatographic analysis on the sample solvent and the reference solvent, and calculating the content of the chlorogenic acid, the vitexin, the isovitexin, the hyperoside, the rutin and the quercetin in the canarium pimela leenh. leaves. According to the method for simultaneously measuring the content of the six ingredients in the canarium pimela leenh. leaves, main active ingredients of the canarium pimela leenh. leaves can be directly, rapidly and effectively measured, high detection precision, accuracy and reproducibility are ensured, and a strong scientific basis is provided for further development of the canarium pimela leenh. leaves.

The invention discloses applications of flavone C-glycoside monomer compounds vitexin, isovitexin, orientin and isoorientin in preparation of analgesic drugs and drugs for promoting wound healing, wherein the analgesic activity of the isoorientin is remarkably higher than the analgesic activity of aspirin and rotundine, and orientin and the vitexin have strong effect of wound healing promoting.