20 results about "Isovitexin" patented technology

The invention discloses an HPLC (High Performance Liquid Chromatography) specific chromatogram of Xiaoyin capsules / granules as well as construction and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and chlorogenic acid, gallic acid, hydroxysafflor yellow A, albiflorin, rutin, isovitexin, 5-O-methylvisamidoside, 3, 5-di-O-caffeoylquinic acid, 4, 5-di-O-caffeoylquinic acid, benzoic acid, quercetin, paeonol, kushenone and obakunone are taken as reference substances; the characteristic chromatogram is successfully constructed for detecting different batches of Xiaoyin capsules / particles. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least 14 components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be accurately, rapidly and simultaneously performed on a plurality of characteristic components of the Xiaoyin capsules / particles, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the Xiaoyin capsules / particles.

A MATZHU is prepared by using leaves of Gramineae (Graminae) and Bambusoideae plant as raw materials, and has a stable emerald color, and has an average powder particle size of 800 to 10,000 meshes, and has a total amount of dietary fiber of ≥60%, a content of lignin of ≥20% and a content of minerals of ≥7%, and comprises at least three or more bamboo leaf characteristic components, such as orientin, isoorientin, vitexin, isovitexin, adenosine, δ-hydroxylysine and p-coumaric acid. The method for the MATZHU preparation comprises, in turn, performing blanching and color protection, drying and superfine grinding the raw materials. By utilizing the thermal stability and the light stability of the MATZHU, the MATZHU may be used as a raw food material, a functional ingredient, or a dietary supplement.

The invention relates to the technical field of medicine detection. The invention provides a method for detecting the content of plumbagin and isovitexin in plumbago zeylanica. The method comprises the following steps: (1) respectively preparing standard substance solutions from plumbagin and isovitexin; (2) extracting a sample to be detected to obtain an extracting solution; (3) respectively carrying out liquid chromatography on the plumbagin standard substance solution and the isovitexin standard substance solution to obtain standard curves; and (4) carrying out liquid chromatography detection on the extracting solution, and obtaining the contents of plumbagin and isovitexin in the sample according to the standard curve. The detection method meets the requirements of methodology verification, the plumbagin and the isovitexin can appear peaks before 35 min, the detection result is efficient and accurate, the applicability is high, and the method can be used for quality control of the medicinal material. In addition, the detection method fills the blank of quantitative control of the active ingredient isovitexin in the plumbago zeylanica medicinal material, can more comprehensively reflect the quality condition of the medicinal material, and ensures the quality stability of the medicinal material.

The invention belongs to the technical field of biology. The invention particularly relates to application of the tea tree internode length related gene CsUGT708AC25 in reducing the internode length of a poplar and a tea tree. The invention discloses application of a seedling stage molecular marker of a tea tree internode length associated gene CsUGT708AC25. The seedling stage molecular marker is used for identifying whether the tea tree internode length associated gene CsUGT708AC25 belongs to a short internode plant or not. The invention also provides a CsUGT708AC25.1 gene. The application of the CsUGT708AC25.2 gene is as follows: the internode length of a plant is negatively regulated; and the CsUGT708AC25.1 gene and the CsUGT708AC25.2 gene are silenced, so that the content of vitexin and isovitexin in the tea tree body can be inhibited.

The invention relates to the technical field of plant processing, in particular to a preparation method of a typical bamboo leaf C-glycoside flavone magnetic molecularly imprinted polymer. The preparation method comprises the following steps: designing bamboo leaf carbon glycoside flavone as a template molecule, and preparing a magnetic molecularly imprinted nano polymer MIP with a core-shell structure by adopting a surface molecular imprinting technology; the characterization of TEM, XRD, VSM, FT-IR and the like proves that the prepared MIP is successfully synthesized, and the satisfactory adsorption capacity (for template molecules of 16.10 mg / g) is obtained. The MIP is added into the bamboo leaf flavonoid crude extract, and the total flavonoid content obtained after adsorption is increased from 24% to 90% or above. Six flavonoid compounds are detected from the high-purity flavonoids through HPLC (High Performance Liquid Chromatography), and four C-glycoside flavonoids account for 90.73%; the content of the rubisoside and the isorubisoside is 33-40%, the content of the vitexin and the isovitexin is 40-48%, and the value and the biological activity of the bamboo leaf flavonoid extract are remarkably increased.

The present invention relates to a composition for treating obesity, comprising a wasabi leaf extract, which is a natural extract, as an active ingredient.　The present invention relates to a composition for preventing, ameliorating or treating obesity, comprising a wasabi leaf extract, wherein the wasabi leaf extract includes 0.8-1.6 mg / g of isovitexin and 4.8-9.6 mg / g of sinigrin, and the weight ratio of isovitexin to sinigrin in the composition is 1:1-1:6 (isovitexin: sinigrin). The composition of the present invention exhibits the effects of reducing body weight, body fat, and abdominal fat without causing appetite reduction.

The present invention discloses a method and application thereof for analyzing the active ingredients of Radix Trichosanthis for treating acute pancreatitis based on HPLC-Q-TOF-MS, network pharmacology, and molecular docking. The analysis method uses HPLC-Q-TOF-MS / MS analysis technology to generate a molecular network of chemical components through accurate mass number, secondary mass spectrometry data, mass spectrometry fragmentation rules, and a database. Then, using a network pharmacology-related database, acute pancreatitis disease-related targets are obtained, a "component-disease-target" network diagram is constructed, a protein interaction network is constructed, and GO function and KEGG pathway enrichment analysis are performed. The 10 key chemical components obtained are: quercetin, coumarin glucoside, gentiopicroside, eriodictyol-7-O-glucoside, protocatechuic acid, 3-O-caffeoylquinic acid, astragalin, isoorientin, isovitexin, and mangiferin.

This invention discloses the application of isovitexin and its composition in products. The isovitexin and its composition can be used in the preparation of products with oil-controlling effects. The addition amount of isovitexin in the product is 1-10 μg / mL. The isovitexin composition comprises isovitexin and resveratrol, with a mass ratio of isovitexin to resveratrol of 1:9-9:1. The addition amount of the isovitexin composition in the product is 10 μg / mL. This invention has the advantage of being applicable to the preparation of products with oil-controlling effects.

The invention discloses a lophatherum gracile and sorghum sweet wine production process based on a subcritical technology. The lophatherum gracile and sorghum sweet wine production process comprises the following steps: mixing cleaned pest-free sorghum and lophatherum gracile with water; putting the mixture into a subcritical kettle, and treating by adopting a gradient heating / variable pressure process, so as to improve the dissolution rate of flavone and effectively degrade tannin; cooling the heated mixture to 30-40 DEG C, and inoculating sweet wine koji for fermentation; after fermentation, filtering the fermented mash to obtain fermentation liquor; and heating and sterilizing the fermentation liquor, and cooling to obtain the lophatherum gracile and sorghum sweet wine. According to the process, the raw material soaking and cooking time in a traditional sweet wine brewing process can be shortened, and the production efficiency of the lophatherum gracile and sorghum sweet wine is improved; meanwhile, tannin substances in sorghum are degraded by adopting a subcritical technology, and the obtained lophatherum gracile and sorghum sweet wine is free of bitter taste; thirdly, dissolution of flavonoid compounds such as isovitexin and isoorientin in lophatherum gracile is accelerated by adopting a subcritical technology, and independent extraction is not needed.

The invention belongs to the field of modernization of traditional Chinese medicines, and particularly relates to a preparation method and a detection method of a cuneate lespedeza herb extract and a preparation thereof. The preparation method of the lespedeza cuneata extract comprises the following steps: decocting lespedeza cuneata decoction pieces, and carrying out solid-liquid separation to obtain decoction, namely the lespedeza cuneata extract. The lespedeza cuneata extract preparation is prepared by adding auxiliary materials into the obtained lespedeza cuneata extract. The detection method for the content of the isovitexin component in the lespedeza cuneata extract and the preparation thereof and the specific chromatogram detection method have good specificity, precision, stability and repeatability. Through thin-layer chromatography identification, determination of the content of the index components and establishment of the characteristic chromatogram, the quality of the cuneate lespedeza herb extract and the preparation thereof can be more effectively evaluated, and meanwhile, a scientific and reasonable basis is provided for formulating process standards and quality standards of the cuneate lespedeza herb extract and the preparation thereof.

The application discloses a prepared product of king-stargrass and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparation. The method comprises the following steps: S101, blanching: blanching the king-stargrass in boiling water for 2-4 minutes until the seed coat has small cracks, quickly taking out, and draining water until the water content is 45-60%; S102, airing: airing the king-stargrass until the water content is 25-35%; S103, soaking: soaking the king-stargrass with vegetable oil for 2-3 hours, and the dosage of the vegetable oil is 8-15% of the weight of the king-stargrass; S104, frying: frying the king-stargrass at 165-180 DEG C for 30-40 seconds until the king-stargrass is completely popped; and S105, cooling: cooling the king-stargrass. The popping rate of the prepared product of the king-stargrass is 90-94%, the content of king-stargrass flavonoid glycoside is between 0.2-0.3%, the content of isovitexin-2''-O-arabinoside is greater than 0.08%, and the content of fatty oil is 5.0-5.7%.

The invention discloses a duckweed LtF3 'H gene and application thereof. Relates to the technical field of gene engineering. The invention provides a gene sequence, an amplification primer and application. The key function of the F3 'H gene in plant secondary metabolites is that F3' H can catalyze naringenin to generate eriodictyol, and eriodictyol can influence accumulation of anthocyanidin, so that the content of cyanidin-3-o-glucoside in a duckweed LtF3 'H overexpression strain is remarkably increased, meanwhile, F3' H can catalyze apigenin to synthesize luteolin, and the yield of luteolin is increased. The luteolin is a mother nucleus of orientin and isoorientin, so that the contents of orientin and isoorientin in an overexpression strain of the duckweed LtF3 'H are remarkably increased, and meanwhile, the content of apigenin C-glycoside (vitexin and isovitexin) is reduced.

The present application relates to the technical field of pharmaceutical chemistry, in particular to a preparation method and application of a berberine and isovitexin nanomedicine. The application is used in the preparation of a drug for treating diseases related to inflammation mediated by neutrophils. The berberine and isovitexin or the structural analogues of berberine and isovitexin form nanoparticles in a carrier-free form through self-assembly. The berberine and isovitexin nanomedicine has a synergistic anti-inflammatory and antioxidant effect, can efficiently inhibit key inflammatory mediators, realize high-efficiency anti-inflammatory and antioxidant synergy, and significantly improve the effect of preventing and treating acute inflammation.

This invention relates to the field of traditional Chinese medicine component analysis technology, specifically to the fingerprint spectrum of Juanxiao tablets and its establishment method. The method for establishing the fingerprint spectrum of Juanxiao tablets includes: using isoharonidin, isovitexin, rutin, hesperidin, lemon balm glycoside, aloe-emodin, nobiletin, and citrus reticulataein as reference standards, and Juanxiao tablets as the test sample; using HPLC to detect the reference standards and the test sample, and then comparing and analyzing them to determine the common characteristic peaks of Juanxiao tablets; wherein the HPLC conditions include: a C18 column, mobile phase A as a nitrile solvent, mobile phase B as a 0.05-0.15% phosphoric acid solution, and gradient elution. This method can effectively detect characteristic peaks, reduce the drying of impurity peaks, and the obtained fingerprint spectrum has high resolution and good peak shape. All characteristic peaks are baseline separated, enabling a more comprehensive and accurate evaluation of the quality of Juanxiao tablets.

The invention discloses a selenium-rich mung bean product with high gamma-aminobutyric acid content and a preparation method of the selenium-rich mung bean product. The method comprises the following steps: firstly, soaking mung beans in a selenium-rich solution, and then carrying out microwave treatment to enable the mung beans to be rich in selenium; then, germination is carried out in a low-oxygen environment in combination with acid and salt stress conditions, physiological metabolism is regulated and controlled, and enrichment of gamma-aminobutyric acid, conversion of organic selenium and synthesis of flavonoid components are promoted; and finally, dehydrating and baking the germinated mung beans to prepare the mung bean product rich in gamma-aminobutyric acid and organic selenium. The production process is simple, the content of gamma-aminobutyric acid, organic selenium, vitexin, isovitexin and other flavonoid compounds in the mung beans is remarkably increased through selenium-rich solution soaking and hypoxia combined acid salt stress germination, and the palatability and fragrance are enhanced through a high-temperature baking process after dehydration. The mung bean product disclosed by the invention can be used for producing tea bags for direct brewing and drinking, and can also be prepared into powder to serve as a functional food ingredient, so that the requirements of consumers on healthy foods are met.

The invention relates to the technical field of medicinal material identification, in particular to a rapid lavender germplasm identification method and application of isovitexin in lavender edulis identification, the rapid lavender germplasm identification method comprises the following steps: step 1, extracting a sample to be detected to obtain an extracting solution of the sample to be detected; 2, detecting the content of isovitexin in the to-be-detected sample extracting solution; step 3, determining whether the to-be-detected sample contains lavandula angustifolia or not according to the content of isovitexin in the to-be-detected sample extracting solution. The systematic metabonomics research finds and confirms that isovitexin serves as a characteristic marker for lavender germplasm identification, lavandula dentata and lavandula angustifolia can be rapidly identified, and the method is easy and convenient to operate, low in cost, good in stability and reliability and convenient to apply and popularize.

The invention discloses a preparation and quality detection method of a Pimpinella diversifolia fruit standard decoction, and particularly provides a detection method of the Pimpinella diversifolia fruit standard decoction, which comprises the following steps: detecting the Pimpinella diversifolia fruit standard decoction by high performance liquid chromatography; the pimpinella diversifolia fruit standard decoction is prepared from chlorogenic acid, isoorientin, isovitexin, luteolin and apigenin. Chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is an octadecyl bonded silica gel chromatographic column; the mobile phase A is acetonitrile, and the mobile phase B is 0.05-0.1% acetic acid aqueous solution; and gradient elution. The method is good in stability, high in precision and good in reproducibility. The fingerprint spectrum of the standard decoction of the pimpinella diversifolia fruit decoction pieces has seven common peaks, five components are determined through identification and are all main effective components of the pimpinella diversifolia fruit, a basis can be provided for establishment and quality control of the fingerprint spectrum of the formula granules, and the clinical effectiveness of the pimpinella diversifolia fruit formula granules is ensured.

The invention belongs to the technical field of biological medicines, and particularly relates to application of isovitexin in resisting porcine circovirus diseases. It is found through in-vitro and in-vivo experiments that the isovitexin has the effect of resisting the porcine circovirus (especially PCV2) for the first time, proliferation and replication of the PCV2 can be remarkably inhibited, Cap protein expression of the porcine circovirus can also be inhibited, lung pathological injuries caused by porcine circovirus infection are relieved, the medicinal value of the isovitexin is expanded, and the clinical application prospect is wide. And an important foundation is laid for the development of high-efficiency and low-toxicity anti-PCV2 drugs. Besides, the isovitexin has the advantages of wide source, convenience in extraction, stable performance, high bioavailability, weak toxicity, even no toxicity and the like, can effectively reduce the medication cost when being used for preventing, relieving or / and treating porcine circovirus infection, is economical and feasible, and has a potential application prospect.

The invention discloses a composition capable of relieving exercise fatigue and a preparation method thereof. The composition is prepared from 10-20 parts by weight of clinacanthus nutans flavone and 10-20 parts by weight of ice clara polysaccharide, and the optimal ratio is 2: 1. The clinacanthus nutans flavone mainly comprises six flavone components including schaftoside, vitexin, isovitexin and the like, and is extracted through a special process. The ice clara polysaccharide is obtained by the processes of water extraction and alcohol precipitation, deproteinization, macroporous resin separation and the like. The action mechanism of the composition is based on a multi-target collaborative intervention strategy: establishing a complementarity anti-oxidation defense system; energy supply and utilization are optimized; the removal of metabolites such as lactic acid is accelerated; two-way regulation of'anti-inflammatory-immune 'balance is formed; and meanwhile, nerve-muscle system protection is provided. Experiments prove that the composition can improve exercise fatigue by promoting glucose utilization and protein synthesis level of C2C12 myotubular cells.

The present application relates to a kind of methods for constructing transgenic tobacco plants capable of heterologous synthesis flavonoid carbon glycoside substance and its application, belong to genetic engineering technical field.The present application provides EbCHS Gene, EbCHI Gene, PhF2H Gene, PhF3' H Gene and ZmUGT708A6 The application of gene in heterologous synthesis flavonoid carbon glycoside.The present application scheme is by chemical synthesis EbCHS 、 EbCHI , PhF2H 、 PhF3' H And ZmUGT708A6 Flavonoid carbon glycoside synthesis related gene, constructs multi-gene expression vector, is successfully obtained by agrobacterium-mediated transformation transgenic tobacco capable of expressing multiple genes, by determination, the transgenic tobacco can successfully synthesize four kinds of flavonoid carbon glycoside such as vitexin, isovitexin, sophorin and isosophorin, provides technical support for natural medicine efficient preparation and medicinal plant germplasm innovation.