55 results about "Isoorientin" patented technology

The invention provides a Tibetan virgate wormwood herb extract containing swertiamain, gentiopicroside, sweroside, mangiferin and isoorientin, and the weight ratio of the five ingredients is (0.04-0.71): (20-40): (1-15): (1.6-26): (0.01-0.16). The invention further provides a preparation method of the Tibetan virgate wormwood herb extract, a pharmaceutical composition containing the Tibetan virgate wormwood herb extract and a medical use of the Tibetan virgate wormwood herb extract. The Tibetan virgate wormwood herb extract can improve various indicators caused by liver injury, promote the reversal of the liver fibrosis process and better prevent liver cirrhosis.

A method for extracting and purifying a flavonoid glycoside monomer from a sensitive plant belongs to the technical field of Chinese traditional medicine. The technical scheme is as following: taking the sensitive plant as a raw material; performing ultrasonic extraction and concentration, and then performing alcohol precipitation to remove impurities and reconcentration; separating and enriching by macroporous resin column chromatography, and then separating by combing a gel resin column chromatography technology and a silica gel column chromatography technology to obtain three monomeric compounds including vitexin, orientin and isoorientin, wherein, the three monomeric compounds have the extraction yield of 1.12%, 2.62% and 2.21% respectively, and the purity of the three is above 95%. The method has the advantages of convenient operation, low cost, high purity, high yield, repetitive recycle of the macroporous resin and the solvent, disuse of toxic solvents in the process, no environmental pollution, easy industrialized production and the like.

The invention provides novel flavone hydroxylase, a microorganism for synthesizing a flavone C-glycoside compound and an application of the novel flavone hydroxylase. The inventor clones to obtain novel flavone hydroxylase PhF2H and PhF3'H, and the novel flavone hydroxylase PhF2H and PhF3'H belongs to cytochrome P450 hydroxylase and has a function of hydroxylating a specific position of the compound. Furthermore, the inventor efficiently synthesizes the flavone C-glycoside compound, such as orientin, isoorientin, vitexin and isovitexin, and related intermediates such as eriodictyol, 2-hydroxynaringenin and the like in a synthetic route of the flavone C-glycoside compound in an artificial recombinant expression system by modifying the enzyme and combining with the assembly of a synthetic route of C-glycoside glycosyltransferase and a flavone precursor.

The present invention provides a cannabis-based flavonoid pharmaceutical composition including any one or more selected from the group of Apigenin, Cannflavin A, Cannflavin B, Cannflavin C, Chrysoeriol, Cosmosiin, Flavocannabiside, Kaempferol, Luteolin, Myricetin, Orientin, Isoorientin (Homoorientin), Quercetin, (+)-Taxifolin, Vitexin, and Isovitexin, or their synthases, for the prevention and treatment of certain diseases of the CNS system and related disorders.

The invention relates to a gentiana macrophylla capsule fingerprint spectrum and an application of the spectrum to quality control and component analysis. According to the spectrum, a HPLC (high-performance liquid chromatography) fingerprint spectrum common mode of a gentiana macrophylla capsule is determined by the aid of a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system (2004A), 23 common peaks are calibrated, similarity is 0.966-0.982, and the content of loganic acid, shanzhiside methyl ester, 6'-O-beta-D-glucose gentiopicroside, swertiamarin,gentiopicroside, sweroside, isoorientin and isovitexin in 10 preparations is measured. According to the spectrum, a HPLC fingerprint spectrum method of the gentiana macrophylla capsule and a content measuring method are built, the method has good analysis evaluation ability and the advantages of accuracy, convenience, stability, reliability and the like, so that the method can serve as an effective evaluation method for the quality of the preparation.

A quality control method for the compound Hongcao medicine in the form of freeze-dried powder injection, injection, or orally taken medicine features that an efficient liquid-phase chromatography is used to measure the contents of scutelloside, isoorientin and orientin.

Provided is a production process for extracting isoorientin from bamboo product waste, including cleaning the bamboo in waste of bamboo product processing, removing impurities, extracting by reflux, concentrating under reduced pressure, dissolving by reflux, concentrating under reduced pressure, cooling, drying the solid and dissolving, passing through a silica gel short column, eluting, concentrating under reduced pressure, crystallizing to get coarse crystal, then redissolving, and crystallizing by freezing to get isoorientin crystal. The production process according to the invention can take advantage of bamboo as waste to extract isoorientin to achieve the purposes of reducing waste of natural resources and reducing environmental pollution.

The invention discloses a gentianella acuta extract for treating arrhythmia. The extract consists of ketone compounds, iridoid compounds and flavonoid compounds. The invention also discloses a preparation method and application of the gentianella acuta extract. The gentianella acuta extract disclosed by the invention comprises 20-40% of ketone compounds based on the total weight of the extract, 5-15% of iridoid compounds based on the total weight and a small amount of flavonoid compounds, wherein the ketone compounds include 1,3,5,8-tetrahydroxy tetrahydro-ketone, nor-chiratin, 3,8-O-beta-D-diglucopyranose-1,5-dihydroxy-keton, nor-bellidifolin, 1,3,5-trihydroxy-ketone, 2-O-beta-D-n glucopyranose-1-hydroxy-5,6-dimethoxy-ketone and 1,2,8-trihydroxy-5,6-dimethoxy-ketone; the iridoid compounds include gentiopicroside, swertiamarin and decentapicrin; the flavonoid compounds include isoorientin and isovitexin. Chemical and pharmacological experiments prove that the gentianella acuta extract can be used for treating arrhythmia caused by coronary heart disease and ischemic heart disease. The gentianella acuta extract can be made into drugs of various dosage forms independently or by compatible combination or can be used as a raw medicine.

The invention, which belongs to the technical field of food quality control, specifically provides a method for measuring contents of three kinds of components in the aspalathus linearis (brum.f.) r.dahlgren, the aspalathus linearis (brum.f.) r.dahlgren extractive, and the aspalathus linearis (brum.f.) r.dahlgren tea. The method comprises: step one, preparing a mixed reference solution; step two,preparing a test sample solution; step three, carrying out chromatographic condition and system adaptability testing; to be specific, carrying out measurement based on the high performance liquid chromatography under the conditions of using the octadecylsilane chemically bonded silica column as a column, acetonitrile as a mobile phase A, an acetic acid solution with the volume concentration of 0.8to 1. 5% or formic acid with the volume concentration of 0.1 to 0.5% as mobile phase B; carrying out chromatographic analysis according to a gradient elution procedure under conditions of controllingthe flow rate of the mobile phase of 0.8 to 1.2 ml / min and the detection wavelengths of 275 to 295nm and 340 to 360nm; and step four, performing a measuring method; to be specific, abstracting the mixed reference solution and the test sample solution respectively and injecting the solutions into a liquid chromatograph and measuring and calculating contents of isoorientin, orientin, and aspalathine. The invention provides a method for measuring contents of three kinds of components in the aspalathus linearis (brum.f.) r.dahlgren, the aspalathus linearis (brum.f.) r.dahlgren extractive, and theaspalathus linearis (brum.f.) r.dahlgren tea rapidly, accurately, and conveniently.

The invention belongs to and relates to a novel gentiana capsule optimized medicinal composition as well as application thereof in analgesia, anti-inflammatory and cholagogic aspects. The invention provides a gentiana capsule optimized medicinal composition; the gentiana capsule optimized medicinal composition is prepared from the following effective components: 120 to 150 parts of loganic acid, 5to 10 parts of shanzhiside methyl ester, 40 to 45 parts of 6'-O-beta-D-glucosyl gentiopicrin, 10 to 15 parts of swertiamarine, 500 to 520 parts of gentiopicrin, 8 to 10 parts of sweroside, 1 to 2 parts of isoorientin and 1 to 2 parts of isovitexin. The medicinal composition has the advantages that the activity of the medicinal composition in the analgesia, anti-inflammatory and cholagogic aspectsis obviously superior to that of the gentiopicrin, the gentiana capsule and gentian total glycoside extract at the aboveground part of the gentian.

The invention provides a method for enhancing the light stability of isoorientin and application, and belongs to the technical field of chemical material performance improvement. The method is characterized in that: isoorientin is attached to the surfaces of silver nanoparticles to form the silver nanoparticles loaded with isoorientin, so that an isoorientin-silver nanoparticle compound is prepared. Compared with pure isoorientin, the photostability of the isoorientin-silver nanoparticle compound is greatly improved.

The present invention belongs to the technical field of food quality control, and especially provides a Rooibos tea and a content determination method of effective components in a product of Rooibos tea. The method comprises the following content determination steps of isoorientin: A: content determination of isoorientin; preparation of an isoorientin reference solution; preparation of a test solution; and chromatographic condition and system suitability test: performing determination according to the high-performance liquid chromatography by employing a Waters Symmetry C18 (4.6mm*250mm, 5[mu]m) chromatographic column or a Diamonsil C18 (4.6mm*250mm, 5[mu]m) chromatographic column, taking acetonitrile as a mobile phase A, taking an acetum with a volume concentration of 0.8-1.5% or formic acid with a volume concentration of 0.1-0.5% as a mobile phase B, performing chromatographic analysis according to a gradient elution program, controlling the flow speeds of the mobile phases in a range of 0.8-1.2ml / min, and controlling the detection wavelengths of the mobile phases in ranges of 275-295nm and 340-360nm, and determining and calculating the content of the isoorientin in the test solution. The Rooibos tea and the content determination method of effective components in a product of Rooibos tea are fast, accurate and convenient for determination of the content of the effective components in a product of Rooibos tea.

The invention provides a method for determining the content of active ingredients in Chinese honeylocust fruit, which adopts ultra-high performance liquid chromatography-mass spectrometry. The content of L-malic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, scopoletin, liquiritigenin, apigenin, luteolin, eriodictyol, citrus aurantium, fustin, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, vitexin, isovitexin, quercitrin, orientin and isoorientin in the Chinese honeylocust fruit can be rapidly detected. The method provided by the invention has the advantages of simple operation, high sensitivity, fast analysis speed and strong specificity, and can be used for quality control of Chinese honeylocust fruit.

The invention discloses application of isoorientin in preparation of a medicament for resisting respiratory syncytial virus. The medicament for resisting the respiratory syncytial virus contains isoorientin with effective treatment dose and pharmaceutically acceptable carrier. The medicament for resisting the respiratory syncytial virus is prepared into powder, pills, tablets, capsules, oral liquid, aerosol or injection. Aiming at the problem to be emergently solved in the field of treating the current respiratory virus, the invention seeks a new efficient and low-toxicity respiratory virus resistant medicament resource; the isoorientin provided by the invention is a natural monomer compound, and has strong activity for resisting the respiratory syncytial virus and low toxicity on host cells; in addition, in vitro anti-virus experiments show that the isoorientin applied with virazole serving as a clinically common anti-virus medicament has synergistic action. Therefore, the isoorientin has the characteristics of efficient anti-virus activity, low toxicity and novel acting mechanism, and has good application prospect.

A method for extracting and purifying a flavonoid glycoside monomer from a sensitive plant belongs to the technical field of Chinese traditional medicine. The technical scheme is as following: taking the sensitive plant as a raw material; performing ultrasonic extraction and concentration, and then performing alcohol precipitation to remove impurities and reconcentration; separating and enrichingby macroporous resin column chromatography, and then separating by combing a gel resin column chromatography technology and a silica gel column chromatography technology to obtain three monomeric compounds including vitexin, orientin and isoorientin, wherein, the three monomeric compounds have the extraction yield of 1.12%, 2.62% and 2.21% respectively, and the purity of the three is above 95%. The method has the advantages of convenient operation, low cost, high purity, high yield, repetitive recycle of the macroporous resin and the solvent, disuse of toxic solvents in the process, no environmental pollution, easy industrialized production and the like.

The invention discloses a pharmaceutical composition for lowering urine protein and an application of the pharmaceutical composition. The composition is prepared from active ingredients and auxiliary materials, wherein the active ingredients include orientin and / or isoorientin. The pharmaceutical composition disclosed by the invention has the effect of lowering urine protein, and especially can be used for treating a nephrotic patient with minimal lesion.

The invention belongs to the technical field of organic complex preparation, and relates to a water-soluble isoorientin-zinc complex and a preparation method and application thereof. The preparation method includes the steps: 1), adding absolute ethyl alcohol into isoorientin, then adding zinc acetate after isoorientin is dissolved, and adjusting the pH to 7.5-8.5 with a NaOH-ethyl alcohol mixed solution; 2) carrying out water bath reflux magnetic stirring, cooling, aging, and carrying out precipitation centrifugal separation to obtain a precipitate and a supernatant; 3) washing the precipitate with absolute ethyl alcohol, and carrying out vacuum freeze drying to obtain the isoorientin-zinc complex, wherein the water contact angle of the isoorientin-zinc complex is 17.4 degrees, and the isoorientin-zinc complex is applied to inhibition of escherichia coli and staphylococcus aureus. The isoorientin-zinc complex is good in water solubility and biological activity, good in biological safety and high in oxidation resistance, has a good inhibition effect on staphylococcus aureus and escherichia coli, and can be widely applied to the food and medicine industries.

The invention belongs to the technical field of plant extraction and medicine, and provides a fernleaf hedge bamboo extract, and a preparation method and application thereof. The preparation method ofthe fernleaf hedge bamboo extract comprises the following steps: with fernleaf hedge bamboo leaves as a raw material, extracting the fernleaf hedge bamboo leaves to obtain a fernleaf hedge bamboo leaf extract; adding acid into the fernleaf hedge bamboo leaf extract to enable the final concentration of hydrogen ions in the fernleaf hedge bamboo leaf extract to reach 0.25-2 mol / L, and then carryingout an acid hydrolysis reaction to obtain a hydrolysate; and carrying out extraction separation on the hydrolysate to obtain the fernleaf hedge bamboo extract. According to the method, hydrochloric acid or sulfuric acid is added into the fernleaf hedge bamboo leaf extract for acid hydrolysis reaction, so the content of other bamboo leaf flavonoids and other components in the fernleaf hedge bambooextract can be reduced, the content of isoorientin and vitexin in the fernleaf hedge bamboo extract can be increased, and convenience is brought to purification of isoorientin and vitexin monomers.

The invention discloses application of a traditional Chinese medicine active ingredient, namely, babybreath isoorientin to preparation of medicine for treating chemical hepatic fibrosis. Treatment of babybreath isoorientin for chemical hepatic fibrosis is researched for the first time. Experiments indicate that babybreath isoorientin can act on multiple links or target points of chemical hepatic fibrosis, lipid peroxidation reactions can be effectively restrained, hepatic tissue pathological damage can be effectively relieved, collagen deposition can be effectively restrained, the activity of matrix metalloproteinase can be effectively adjusted and controlled, and an NF-kappa b signal transduction pathway can be effectively blocked; hepatic injury is obviously relieved, and special advantages are achieved for preventing and treating hepatic injury. Babybreath isoorientin has an obvious restraining effect on hepatic injury caused by carbon tetrachloride and is new medicine for treating chemical hepatic fibrosis, and the new medicine has potential value.

The invention discloses a preparation method of a sensor for detecting vanillyl mandelic acid and application of the sensor. The electrochemical sensor comprises a glassy carbon electrode, and a sensing film formed by isoorientin is dropwise coated and electrodeposited on the surface of the glassy carbon electrode. The preparation method includes: 1), dropwise coating the surface of the glassy carbon electrode which is already activated with isoorientin, and naturally airing; 2), putting the glassy carbon electrode obtained in the step 1) in a phosphate buffering solution for electrochemical polymerization to obtain the sensor. The sensor can avoid interference of a urine matrix, realizes detection of the vanillyl mandelic acid and can be successfully used for sensing detection of the vanillyl mandelic acid in human urine.

The invention discloses a composition, a pharmaceutical preparation and an application. The composition comprises 1-5 parts by mass of ginsenoside Rg2,10-20 parts by mass of at least one of bamboo-leaves flavones, orientoside, Isoorientin, agnoside and isovitexin, and 15-25 parts by mass of taurine or pharmaceutically acceptable salt thereof. The composition can effectively prevent and / or treat cardiovascular and cerebrovascular diseases.

The invention discloses applications of flavone C-glycoside monomer compounds vitexin, isovitexin, orientin and isoorientin in preparation of analgesic drugs and drugs for promoting wound healing, wherein the analgesic activity of the isoorientin is remarkably higher than the analgesic activity of aspirin and rotundine, and orientin and the vitexin have strong effect of wound healing promoting.

The invention provides a Tibetan virgate wormwood herb extract containing swertiamain, gentiopicroside, sweroside, mangiferin and isoorientin, and the weight ratio of the five ingredients is (0.04-0.71): (20-40): (1-15): (1.6-26): (0.01-0.16). The invention further provides a preparation method of the Tibetan virgate wormwood herb extract, a pharmaceutical composition containing the Tibetan virgate wormwood herb extract and a medical use of the Tibetan virgate wormwood herb extract. The Tibetan virgate wormwood herb extract can improve various indicators caused by liver injury, promote the reversal of the liver fibrosis process and better prevent liver cirrhosis.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a traditional Chinese medicine monomer isoorientin for treating diabetic nephropathy, which is a flavonoid compound containing a luteolin structure, exists in medicinal or edible parts of plants such as sow thistle, buckwheat, passion flower and common fenugreek seed, has a molecular formula of C21H20O11, and can be used for treating diabetic nephropathy. The traditional Chinese medicine monomer isoorientin is used for treating diabetic nephropathy, the curative effect is good, no toxic or side effect exists, the isoorientin is wide in source and easy to obtain, and the cost is low.

The invention discloses a preparation method of active Chinese liquor brewed from hyparrhenia, and belongs to the technical field of liquor preparation. The active Chinese liquor comprises the following raw materials in parts by weight: 8 to 15 parts of small cogon grass, 40 to 45 parts of sorghum and 40 to 45 parts of corns. The small cogon grass is used as an auxiliary material of distilled liquor and has the effects of ventilating to increase the liquor yield and improve the liquor quality; the gramineous herbaceous plant contains various elements beneficial to the human body, such as tricin, coixol, isoorientin and other various flavonoid compounds, so that the prepared liquor has the effects of tonifying the spleen-stomach and qi and removing blood stasis; and quartz and premna leavesare added into fermented liquor, so that the activity and the health-care effect of the Chinese liquor are improved. The prepared liquor belongs to plant active liquor, has the effects of building body, maintaining beauty, keeping young, regulating yin and yang, enhancing immunity and prolonging life after being properly drunk by people, can also be used for wiping in the fields of rheumatism andsprain, and can also be applied to the health care fields of scraping therapy, massotherapy and the like.

The invention discloses application of flavonoid carbon glycoside monomeric compounds such as vitexin, isovitexin, orientin and isoorientin in preparation of analgesic drugs and wound healing promoting drugs. The analgesic activity of isoorientin is significantly higher than that of aspirin and rotundine, and orientin and vitexin have a strong wound healing promoting effect.

The invention discloses an application of a flavone C-glycoside monomer in food preservation; and the flavone C-glycoside compound is selected from orientin, isoorientin, vitexin or isovitexin.

The invention discloses an application of a passionflower vine leaf extract in preparation of a medicine for preventing and treating breast cancer. The active ingredients in the passionflower vine leaf extract comprise isoorientin, vitexin and isovitexin flavone. The invention also discloses an extraction method of the passionflower vine leaf extract. The passionflower vine leaf extract can inhibit proliferation of breast cancer cells, can inhibit migration of the breast cancer cells, and can inhibit the cycle of the breast cancer cells; and the passionflower vine leaf extract can be used for preparing the medicine for preventing and treating breast cancer and other anti-cancer medicines.

Isoorientin analogues and related compounds that inhibit glycogen synthase kinase-3β activity are provided as are methods of using these compounds in the treatment of cognitive, neurodegenerative or neurological diseases or conditions, as well as cancer, obesity, diabetes, inflammatory or autoimmune disease, cardiovascular disorder, metabolic syndrome X, hair loss, severe acute respiratory syndrome coronavirus, cocaine addiction, dental caries, bone loss and glaucoma.

A quality control method for the compound Hongcao medicine in the form of freeze-dried powder injection, injection, or orally taken medicine features that an efficient liquid-phase chromatography is used to measure the contents of scutelloside, isoorientin and orientin.