The invention relates to the technical field of
traditional Chinese medicine detection, in particular to a method for identifying species of fructus viticis and application of the method. When 1.5, the fructus viticis is single-leaf fructus viticis; when the ratio is gt; when 2.0, the fructus viticis is the three-leaf fructus viticis; when the ratio is between 1.5 and 2.0, it is determined that the sample is mixed or abnormal, the
content determination method of the
isoorientin and
vitexin in the indexes is provided in detail, the precision, the stability and the
repeatability of the method are good, and the average sample adding
recovery rate is 80%-120%; through chemical
verification, the identification accuracy reaches 100%, and the index has extremely high specificity on single-leaf fructus viticis; the ratio intervals of the pure single-leaf and three-leaf chastetree fruits are not overlapped, the critical value is clear, and cross misjudgment is avoided, so that the distinguishing degree of the identification index is extremely high, the ratio calculation result is stable, abnormal discrete data is avoided, and the objectivity and reliability of a
verification conclusion are ensured.