20 results about "Isoorientin" patented technology

The invention discloses a ferroptosis inhibitor and application of the ferroptosis inhibitor in radiation pneumonitis drugs, and relates to the technical field of biological medicines. The specific preparation method comprises the following steps: adding the ferroptosis inhibitor and the auxiliary agent into the solvent, and dissolving to obtain the ferroptosis inhibitor composition; wherein the ferroptosis inhibitor is isoorientin; the auxiliary agent is a glucosamine derivative; the solvent is a serum-free DMEM culture medium. The ferroptosis inhibitor composition prepared by the method provided by the invention can effectively reduce the rise of active oxygen, ferrous ions and malondialdehyde levels of human pulmonary epithelial cells BEAS-2b and mouse pulmonary epithelial cells MLE-12 caused by irradiation, restore cell mitochondrial membrane potential enrichment and inhibit the expression of various inflammatory factors in irradiated cells; therefore, the process of radiation pneumonitis is remarkably improved, and a new medication strategy is provided for research on radiation pneumonitis drugs.

The invention discloses a method for improving hydrophobic active substance encapsulation and intestinal slow release through pH treatment of whey protein, and relates to the technical field of functional food processing. The natural food-borne substance whey protein is subjected to pH pretreatment, so that a hydrophobic inner cavity of the natural food-borne substance whey protein is better opened, then the natural food-borne substance whey protein and the isoorientin form a self-assembled compound under the driving of hydrogen bonds and hydrophobic action, and the isoorientin is wrapped in the hydrophobic inner cavity of the protein, so that the stability and bioavailability of the isoorientin in the digestion process are improved. Compared with a traditional chemical modification or artificial synthesis delivery technology, the natural concentrated whey protein is used as the carrier, and the isoorientin is non-covalently combined through self-assembly, so that chemical crosslinking is not needed, the safety is higher, and the carrier is natural in source and has higher efficiency, greenness and sustainability.

The invention discloses a flavonoid glycoside compound as well as a preparation method and application thereof. The invention provides a flavonoid glycoside compound 2 ''-O-benzoyl isoorientin. The compound is a new flavonoid glycoside compound which is separated from gentiana rigescens for the first time. The invention further provides a preparation method of the 2 ''-O-benzoyl isoorientin, and further provides application of the flavonoid glycoside compound 2 ''-O-benzoyl isoorientin in preparation of anti-aging cosmetics or drugs and application of the flavonoid glycoside compound 2 ''-O-benzoyl isoorientin in preparation of anti-tumor drugs.

The invention relates to the technical field of medicines, in particular to application of isoorientin in preparation of a medicine for preventing and treating gouty arthritis. According to the invention, a gouty arthritis mouse animal model is constructed, and in-vivo experiments show that the isoorientin can obviously relieve red, swollen, hot and pain caused by acute gouty arthritis. Therefore, the isoorientin can be regarded as a potential medicine for treating the acute gouty arthritis; the effects are mild; the side effects are small, and the like.

The invention discloses a method for performing carbon glycoside deglycosylation by using a biological method. The invention provides application of the protein combination: application in splitting C-C glucosidic bonds; application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. Application in splitting C-C glucosidic bonds; the invention also relates to an application in converting mangiferin into mangiferin aglycone. The protein combination comprises an LE1 protein, an LE2 protein and an LE3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.

The invention relates to the technical field of plant processing, in particular to a preparation method of a typical bamboo leaf C-glycoside flavone magnetic molecularly imprinted polymer. The preparation method comprises the following steps: designing bamboo leaf carbon glycoside flavone as a template molecule, and preparing a magnetic molecularly imprinted nano polymer MIP with a core-shell structure by adopting a surface molecular imprinting technology; the characterization of TEM, XRD, VSM, FT-IR and the like proves that the prepared MIP is successfully synthesized, and the satisfactory adsorption capacity (for template molecules of 16.10 mg / g) is obtained. The MIP is added into the bamboo leaf flavonoid crude extract, and the total flavonoid content obtained after adsorption is increased from 24% to 90% or above. Six flavonoid compounds are detected from the high-purity flavonoids through HPLC (High Performance Liquid Chromatography), and four C-glycoside flavonoids account for 90.73%; the content of the rubisoside and the isorubisoside is 33-40%, the content of the vitexin and the isovitexin is 40-48%, and the value and the biological activity of the bamboo leaf flavonoid extract are remarkably increased.

The present invention discloses a method and application thereof for analyzing the active ingredients of Radix Trichosanthis for treating acute pancreatitis based on HPLC-Q-TOF-MS, network pharmacology, and molecular docking. The analysis method uses HPLC-Q-TOF-MS / MS analysis technology to generate a molecular network of chemical components through accurate mass number, secondary mass spectrometry data, mass spectrometry fragmentation rules, and a database. Then, using a network pharmacology-related database, acute pancreatitis disease-related targets are obtained, a "component-disease-target" network diagram is constructed, a protein interaction network is constructed, and GO function and KEGG pathway enrichment analysis are performed. The 10 key chemical components obtained are: quercetin, coumarin glucoside, gentiopicroside, eriodictyol-7-O-glucoside, protocatechuic acid, 3-O-caffeoylquinic acid, astragalin, isoorientin, isovitexin, and mangiferin.

This invention belongs to the field of pharmaceutical technology, specifically relating to a temperature- and pH-responsive ocular hydrogel loaded with isosorbide nanomicelles, its preparation method, and its applications. This invention is the first to apply the traditional Chinese medicine ingredient ISO to the treatment of cataracts, and innovatively designs a complete ocular delivery formulation for it. This invention not only pioneers a new application field for ISO in ophthalmic treatment, but more importantly, by constructing a "nanomicelle-smart gel" composite platform, it systematically solves the three fundamental technical challenges faced by ISO in ocular applications: poor solubility, low corneal permeability, and short retention time on the ocular surface, thereby transforming its potential pharmacological activity into actual ocular therapeutic efficacy. Therefore, this invention has significant clinical implications and broad market prospects.

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a process optimization method for green extraction of hemsley rockvine root flavonoid glycoside through ultrasonic cooperation with a surfactant. The method comprises the following steps: optimizing process parameters for extracting isoorientin, isoquercitrin, kaempferol-3-O-rutinoside and astragalin from radix tetrastigme tuberous roots by using an ultrasonic-assisted lauryl sodium sulfate aqueous solution micelle medium; influences of the type and concentration of a surfactant, a material-liquid ratio, ultrasonic power, ultrasonic time, ultrasonic times and ultrasonic temperature on target components are investigated, and optimal extraction process parameters are determined: the concentration of sodium dodecyl sulfate (SDS) is 35mg / mL, the ultrasonic power is 100W, and the ultrasonic temperature is 50 DEG C. According to the process, a traditional organic solvent is replaced by the surfactant, so that the extraction efficiency is improved; a feasible scheme is provided for green extraction of active ingredients of radix tetrastigme, and industrial amplification feasibility can be further explored subsequently.

The invention relates to the technical field of bamboo leaf detection, and discloses a bamboo leaf detection method based on thin-layer chromatography, which comprises the following steps: S1, preparation of a reference substance solution: respectively taking vitexin, isoorientin and orientin, and adding methanol for dissolving to prepare a mixed solution as a mixed reference substance solution; s2, preparation of a test solution: taking a to-be-detected sample, adding an extraction solvent for extraction, taking supernate, and filtering to obtain the test solution; s3, thin layer chromatography determination: taking the reference substance solution and the test solution, respectively dispensing the reference substance solution and the test solution on the same polyamide film, adding a developing solvent, developing, taking out, airing, spraying a color developing agent, drying until spots are clearly developed, and then placing under an ultraviolet lamp for inspection. According to the method, the bamboo leaves can be rapidly and effectively identified by displaying spots with the same color at the corresponding positions of the reference substance chromatogram, and the method is simple and convenient to operate, high in precision and sensitivity and good in stability, can more accurately identify the bamboo leaves compared with the prior art, and has a good application prospect.

To provide a novel material derived from natural products that is effective in promoting the expression of the PGC-1α gene or the like.SOLUTION: Provided is a food composition or pharmaceutical composition for promoting expression of the PGC-1α gene in myotube cells or in skeletal muscle, which contains isoorientin and at least one of 3-o-caffeoylquinic acid and 5-o-caffeoylquinic acid.SELECTED DRAWING: Figure 2

The invention discloses a lophatherum gracile and sorghum sweet wine production process based on a subcritical technology. The lophatherum gracile and sorghum sweet wine production process comprises the following steps: mixing cleaned pest-free sorghum and lophatherum gracile with water; putting the mixture into a subcritical kettle, and treating by adopting a gradient heating / variable pressure process, so as to improve the dissolution rate of flavone and effectively degrade tannin; cooling the heated mixture to 30-40 DEG C, and inoculating sweet wine koji for fermentation; after fermentation, filtering the fermented mash to obtain fermentation liquor; and heating and sterilizing the fermentation liquor, and cooling to obtain the lophatherum gracile and sorghum sweet wine. According to the process, the raw material soaking and cooking time in a traditional sweet wine brewing process can be shortened, and the production efficiency of the lophatherum gracile and sorghum sweet wine is improved; meanwhile, tannin substances in sorghum are degraded by adopting a subcritical technology, and the obtained lophatherum gracile and sorghum sweet wine is free of bitter taste; thirdly, dissolution of flavonoid compounds such as isovitexin and isoorientin in lophatherum gracile is accelerated by adopting a subcritical technology, and independent extraction is not needed.

The invention discloses a duckweed LtF3 'H gene and application thereof. Relates to the technical field of gene engineering. The invention provides a gene sequence, an amplification primer and application. The key function of the F3 'H gene in plant secondary metabolites is that F3' H can catalyze naringenin to generate eriodictyol, and eriodictyol can influence accumulation of anthocyanidin, so that the content of cyanidin-3-o-glucoside in a duckweed LtF3 'H overexpression strain is remarkably increased, meanwhile, F3' H can catalyze apigenin to synthesize luteolin, and the yield of luteolin is increased. The luteolin is a mother nucleus of orientin and isoorientin, so that the contents of orientin and isoorientin in an overexpression strain of the duckweed LtF3 'H are remarkably increased, and meanwhile, the content of apigenin C-glycoside (vitexin and isovitexin) is reduced.

This invention discloses a method for increasing the content of flavonoids and cyanidin in *Phyllostachys edulis*, belonging to the field of plant cultivation technology. This invention uses continuous irradiation of *Phyllostachys edulis* with light of specific wavelengths and intensities; light with wavelengths of 354-478 nm is the main wavelength inducing culm discoloration; when the blue light intensity exceeds 8 μmol / m², the irradiation is further enhanced. 2 At a certain temperature, the color and anthocyanin content of bamboo increased significantly. After light treatment, the contents of six flavonoids, including vitexin, isohypericin, narcissin, hyperoside, luteolinin, and luteolin, increased by 9.02, 8.79, 2.34, 8.5, 9.4, and 1.9 times, respectively; the content of cyanidin-3-O-rutin increased by 7.41 times.

The invention provides a method for extracting isoorientin from corn stigma, and relates to the technical field of extraction of active ingredients. The method comprises the following steps: carrying out drying and crushing pretreatment on corn stigma, fully extracting corn stigma powder by using a natural eutectic solvent, designing three single factors to be combined with ultrasonic treatment, and carrying out vacuum filtration on the treated sample to further enrich the extract. Compared with a traditional chemical synthesis method for preparing the isoorientin, the isoorientin prepared by the method has the advantages that the purity is higher, chemical substances in the product are fewer, the residual quantity of harmful substances is low, the extraction efficiency of the isoorientin is further improved, effective components in the corn stigma can be quickly and fully dissolved out in a short time, and auxiliary products of corn can be recycled.

The invention discloses application of two small molecular substances in regulation and control of weed suppression activity of flavonoid glycoside substances. The invention provides any one of the following applications of a GST activator and a GST inhibitor: (A1) regulating the inhibition activity of wheat flavone-5-O-glucoside to weeds; and (A2) the inhibition activity of the isoorientin on weeds is regulated. According to the invention, the weed suppression effect of the flavonoid glycoside substances can be obviously improved, and the use of chemical pesticides is reduced, so that a novel biological control method is provided for agricultural production, and green agriculture and sustainable development can be realized.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for identifying species of fructus viticis and application of the method. When 1.5, the fructus viticis is single-leaf fructus viticis; when the ratio is gt; when 2.0, the fructus viticis is the three-leaf fructus viticis; when the ratio is between 1.5 and 2.0, it is determined that the sample is mixed or abnormal, the content determination method of the isoorientin and vitexin in the indexes is provided in detail, the precision, the stability and the repeatability of the method are good, and the average sample adding recovery rate is 80%-120%; through chemical verification, the identification accuracy reaches 100%, and the index has extremely high specificity on single-leaf fructus viticis; the ratio intervals of the pure single-leaf and three-leaf chastetree fruits are not overlapped, the critical value is clear, and cross misjudgment is avoided, so that the distinguishing degree of the identification index is extremely high, the ratio calculation result is stable, abnormal discrete data is avoided, and the objectivity and reliability of a verification conclusion are ensured.

The invention discloses a method for splitting a flavonoid C-C glucosidic bond by using an enzyme method. The invention provides application of a protein combination A or a protein combination B, namely application in splitting C-C glucosidic bonds. Application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. The protein combination A is an LD1 protein, an LD2 protein and an LD3 protein. And the protein combination B is an LD4 protein, an LD2 protein and an LD3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.

The present invention discloses a ferroptosis inhibitor and its application in radiation-induced pneumonia drugs, and relates to the field of biomedicine technology. The specific preparation method comprises: adding a ferroptosis inhibitor and an auxiliary agent to a solvent to dissolve to obtain a ferroptosis inhibitor composition; wherein the ferroptosis inhibitor is isoorientin; the auxiliary agent is a glucosamine derivative; and the solvent is a serum-free DMEM culture medium. The ferroptosis inhibitor composition prepared by the method of the present invention can effectively reduce the increase in the levels of reactive oxygen species, ferrous ions, and malondialdehyde in human lung epithelial cells BEAS-2b and mouse lung epithelial cells MLE-12 caused by irradiation, restore the enrichment of cell mitochondrial membrane potential, and inhibit the expression of various inflammatory factors in irradiated cells, thereby significantly improving the progression of radiation-induced pneumonia and providing a new medication strategy for the research of radiation-induced pneumonia drugs.

The present invention provides a method for constructing a characteristic fingerprint of Swertia chirayita and a method for identifying Swertia chirayita. The characteristic fingerprint of Swertia chirayita includes characteristic peaks of the following substances: isomangiferin, swertiamarin, mangiferin, gentiopicroside, sweroside, isoorientin, methylswerinine, oleanolic acid, and ursolic acid. Using the characteristic peaks in the characteristic fingerprint of Swertia chirayita as a measurement index, Swertia chirayita can be accurately identified and the authenticity can be distinguished. In particular, Swertia chirayita with good curative effects on liver and gall diseases can be screened out, which is helpful for the quality control of Swertia chirayita. Moreover, the construction method and the identification method are easy to operate, and have the advantages of good reproducibility, high precision, and strong stability, and are suitable for popularization and application.