12 results about "Aglycone" patented technology

This invention provides a method for preparing a daidzein nanoparticle delivery carrier, belonging to the field of food processing technology. Using zein, carrageenan, and sodium alginate as raw materials, this invention utilizes ultrasound technology to construct a novel nanoparticle delivery carrier: zein nanoparticles loaded with a carrageenan / sodium alginate bistabilized layer, thereby improving the stability and bioavailability of daidzein nanoparticles. The advantages of this invention lie in the simplicity and environmental friendliness of the antisolvent precipitation method and ultrasound technology. The prepared composite nanoparticles possess stable physicochemical properties, small particle size, relatively concentrated particle size distribution, and high bioavailability. This invention enhances the practical application value of daidzein-loaded proteoglycan nanoparticles in the food processing industry and provides a theoretical basis for the application of daidzein nanoparticle oral delivery systems.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

This invention discloses a method for determining the flavonoid aglycone content in acid-hydrolyzed sea buckthorn leaf extract, belonging to the field of detection technology. The invention involves acid-hydrolyzing sea buckthorn leaf extract to obtain a filtrate containing flavonoid aglycones, which is used as the test solution. High-performance liquid chromatography (HPLC) is used to detect the flavonoid aglycone content in both the test solution and the reference solution. The preparation method of the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin includes: adding sea buckthorn leaf extract to methanol and hydrochloric acid solutions, mixing thoroughly, heating under reflux, cooling, shaking, and filtering to obtain the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin. This invention simplifies the diverse chemical structures of flavonoid glycosides to a few flavonoid aglycones, effectively reducing dependence on expensive and scarce glycoside reference standards, simplifying chromatographic separation, and improving the stability and reproducibility of the method.

This invention provides a red yeast rice fermentation product for eliminating nodules. A group of important compositions for eliminating nodules were screened and obtained, including dihydroquercetin, Tian Shan snow lotus culture, chicken gizzard lining, dandelion, prunella vulgaris, platycodon grandiflorus, and black buckwheat. The culture medium was prepared using the traditional Chinese medicine composition, and red yeast rice was inoculated for fermentation. This process transformed the large active molecules in the traditional Chinese medicine composition that eliminate nodules into small active molecules such as aglycones, thereby enhancing the efficacy of eliminating nodules and significantly improving the therapeutic effect of the traditional Chinese medicine composition in eliminating nodules, thus meeting clinical needs.

The application discloses a method for improving the proportion of genistein in soybean isoflavone glycosides through directional fermentation. Lactobacillus sp. The method is characterized in that Lactobacillus (RS-03027) is used for fermentation after induced pre-culture, and the substrate is fed in batches, so that high selective conversion of isoflavone glycosides to genistein is realized. The strain has high beta-glucosidase activity related conversion capacity, has been preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.21302. The process realizes high accumulation and high selective enrichment of the target product, effectively inhibits non-target aglycone, and has industrial amplification application potential.

This invention discloses a sleep-aid composition based on biotransformation technology, its preparation method, and its applications. The composition comprises jujube seed extract, sea buckthorn compound extract, γ-aminobutyric acid (GABA), and raspberry anthocyanin extract. Through a dual biotransformation process of "directional fiber-pectin dual-enzyme degradation" and "Lactobacillus plantarum-Saccharomyces cerevisiae symbiotic fermentation," the sugar chains of large molecular weight glycosides in the raw materials are broken down, efficiently enriching free small molecular weight jujube seed saponin A and isorhamnetin aglycone with high brain permeability, and achieving matrix synergy with GABA. The composition of this invention is safe and non-addictive, and can be used to prepare drugs or functional foods for sedation and hypnosis, prolonging slow-wave deep sleep, and promoting neural synapse regeneration and repair.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

This invention belongs to the field of biotechnology, specifically relating to the preparation, conversion, and application of a ginsenoside probiotic composition. By adding probiotics capable of degrading ginsenosides to ginseng products, this invention enhances the bioavailability of ginsenosides in the human body by degrading ginsenosides into ginsenoside diol and ginsenoside triol. The core aglycone of this probiotic composition achieves a conversion rate exceeding 80% within one hour, providing better and more convenient treatment options in anti-inflammatory, immune-regulating, and other related fields, thereby increasing therapeutic efficacy.

This invention discloses a loofah extract with antioxidant activity and its preparation method, belonging to the technical field of plant extracts and natural antioxidants. The method uses loofah leaves, loofah peel, or a combination thereof as raw materials. After pretreatment, crushing, and pulping, the materials undergo acidic hypoxia enzyme-inhibiting pretreatment to reduce the activity of polyphenol oxidase and / or peroxidase. Cell wall degrading enzymes are then added for multi-stage enzymatic hydrolysis to release bound polyphenols. Subsequently, an enzyme source with β-glucosidase, β-glucuronidase, and esterase activity is used to perform directional aglycone conversion of the bound polyphenols, converting bound flavonoids and / or bound phenolic acids into free flavonoid aglycones and / or free phenolic acids. Finally, the extract is obtained through alcohol-water extraction, macroporous resin enrichment, microencapsulation, and drying. The extract obtained by this invention has a high proportion of free active ingredients, good antioxidant activity, and good storage stability.

The application discloses a flavonoid aglycone extraction and separation method based on metal ion doped eutectic solvent, relates to the technical field of natural product extraction and separation, and comprises the following steps: mixing a hydrogen bond acceptor, a hydrogen bond donor and a metal ion according to a molar ratio to synthesize a metal ion doped eutectic solvent, and adding water to obtain an MTDES aqueous solution; adding plant powder containing flavonoid aglycone into the MTDES aqueous solution, performing ultrasonic extraction, centrifugal separation, collecting supernatant, and obtaining an extract; mixing the extract with a water-soluble small-molecule organic solvent, adjusting the pH and / or the concentration of salt, centrifugally separating phases, collecting an organic solvent phase, and concentrating to obtain a flavonoid aglycone crude extract. 3+ The application utilizes the catalysis of Al 3+ and the synergistic effect of the DES hydrogen bond network to realize the integration of in-situ hydrolysis of flavonoid glycosides and extraction of aglycone. Compared with the traditional acid hydrolysis-organic solvent extraction process, the method has the advantages of simplified steps, shortened time and reduced solvent consumption.

This application relates to the fields of microbial fermentation and traditional Chinese medicine processing technology, and discloses a product combination and preparation method for improving sleep, including Gastrodia elata powder, Ziziphus jujuba seed powder, Rosa rugosa juice, honey, and a compound microbial agent. The compound microbial agent contains *Lactobacillus plantarum*, *Lactobacillus rhamnosus*, *Lactobacillus casei*, *Pichia pastoris*, and *Saccharomyces cerevisiae*. The preparation method includes steps such as fermentation culture medium preparation, autoclaving, inoculation with the compound microbial agent for constant-temperature fermentation, separation and filtration, and autoclaving. This invention utilizes the synergistic metabolic action of multiple microbial groups to disrupt plant cell walls, converting macromolecular polar glycosides into free aglycones and γ-aminobutyric acid (GABA) that easily penetrate the blood-brain barrier, thereby enhancing the efficacy of central sedation and sleep improvement. In the fermentation system, the metabolites of lactic acid bacteria and yeast undergo esterification to synthesize volatile flavor esters, masking the inherent earthy and bitter tastes of the raw materials, thus improving the sensory quality and drinking compliance of the product.