96 results about "Aglycone" patented technology

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a dammarane sapogenin-isatin derivative and application thereof in anti-tumor treatment. The dammarane sapogenin-isatin conjugate is constructed by taking dammarane sapogenin as a mother nucleus, deriving the dammarane sapogenin and chloroacetic acid and then introducing an indolone structural unit, and R1, R2 and R3 are as described in the claims and the specification. According to the invention, isatin fragments and ginsenoside are coupled for the first time, the anti-tumor activity of the compounds is systematically evaluated, and the action mechanism research of the compounds in colorectal cancer and other tumors is further developed. Results show that the obtained compound has relatively good anti-tumor efficacy, tumor cell selectivity, relatively low toxicity and relatively high bioavailability, and shows good patent medicine potential and wide market prospects.

A unit dosage of an oral composition of an extract of Withania somnifera is disclosed. The extract of Withania somnifera includes total withanolides. The total withanolides includes withanolide glycosides and withanolide aglycones. The extract of Withania somnifera includes about 32% to about 38% by weight of the withanolide glycosides. Methods of preparing the extract of Withania somnifera are disclosed. Methods of treatment by administering the extract of Withania somnifera are disclosed.

The invention discloses a method for preparing a radix pseudostellariae extract through enzymolysis, and belongs to the technical field of plant effective component extraction. The method comprises the following steps: mixing radix pseudostellariae powder with deionized water which is 10 times of the weight of the radix pseudostellariae powder, and decocting for 2-4 hours to obtain filtrate and filter residues; mixing the filter residue, deionized water and combined enzyme to form slurry, and sequentially performing enzymolysis, inactivation and filtration on the slurry to obtain an extracting solution; mixing the extracting solution and the filtrate, hydrolyzing and catalyzing by glucoside hydrolase, and inactivating to obtain hydrolysate; and sequentially performing rotary evaporation and hot air drying on the hydrolysate to obtain the radix pseudostellariae extract. The radix pseudostellariae is subjected to enzymolysis wall breaking by adopting a combined enzyme, release of effective components is promoted, then filtrate and an extracting solution obtained through wall breaking are subjected to hydrolysis to promote generation of aglycone with higher activity, and finally, the radix pseudostellariae extract is obtained through drying treatment.

The invention provides a preparation method of milbemycin oxime A4, which comprises the following steps: under an acidic condition, carrying out hydrolysis disaccharide removal reaction on tenvermectin B to obtain tenvermectin B aglycone; the preparation method comprises the following steps: carrying out silicon-based protection on tenvermectin B aglycone by adopting a silicon-based protection reagent to obtain 5-hydroxyl silicon-based tenvermectin B aglycone; the preparation method comprises the following steps: brominating 5-hydroxyl silicon-based tenvermectin B aglycone by adopting a brominating reagent to obtain 13-bromine-5-hydroxyl silicon-based protected tenvermectin B aglycone; the preparation method comprises the following steps: reducing 13-bromo-5-hydroxyl silicon group protected tenvermectin B aglycone by using a reducing agent to obtain 5-hydroxyl silicon group protected milbemycins A4; the milbemycins A4 protected by the 5-hydroxyl silicon group is subjected to desilicication protection treatment, and the milbemycins A4 is obtained; oxidizing milbemycins A4 by adopting an oxidizing agent to obtain 5-ketomilbemycins A4; the preparation method comprises the following steps: reacting 5-ketomilbemycins A4 with hydroxylamine hydrochloride to obtain milbemycins A4, and efficiently preparing the active component milbemycins A4 single component by taking tenvermectin B as a raw material.

The present invention relates to a functional coffee substitute composition. The coffee substitute food composition comprising a fermented dandelion root extract, a fermented Acanthopanax seed extract, and a fermented soybean extract of the present invention possesses sensory characteristics similar to coffee, and through fermentation, the content of major plant functional components such as isoflavone aglycone, total polyphenols, flavonoids, and saponins increases, L-carnitine is newly synthesized, and since it is a novel coffee substitute food material having antioxidant effects and anti-diabetic activity, it can be utilized as a caffeine-reducing beverage, a beverage that promotes body fat metabolism, an antioxidant-enhanced beverage, and a health functional food.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention discloses lotus leaf powder as well as a preparation method and application thereof, and belongs to the technical field of deep processing of plant raw materials. The preparation method of the lotus leaf powder provided by the invention comprises the following steps: carrying out enzymolysis on pretreated fresh lotus leaves in a system containing beta-glucosidase, and then carrying out scalding and ice water leaching cooling; the obtained lotus leaf powder has bright emerald green color, and contains more active ingredients such as flavonoid aglycones and nuciferine which are beneficial to human body absorption; moreover, the content of tannin and total flavonoid glycoside in the obtained lotus leaf powder is relatively low, so that the lotus leaf powder has relatively low bitter and astringent taste, and the obtained lotus leaf powder is excellent in mouth feel; meanwhile, the obtained lotus leaf powder also has good capability of improving blood fat; the method can be widely applied to preparation of matcha-flavored food or preparation of functional preparations for improving blood fat.

This invention provides a method for preparing a daidzein nanoparticle delivery carrier, belonging to the field of food processing technology. Using zein, carrageenan, and sodium alginate as raw materials, this invention utilizes ultrasound technology to construct a novel nanoparticle delivery carrier: zein nanoparticles loaded with a carrageenan / sodium alginate bistabilized layer, thereby improving the stability and bioavailability of daidzein nanoparticles. The advantages of this invention lie in the simplicity and environmental friendliness of the antisolvent precipitation method and ultrasound technology. The prepared composite nanoparticles possess stable physicochemical properties, small particle size, relatively concentrated particle size distribution, and high bioavailability. This invention enhances the practical application value of daidzein-loaded proteoglycan nanoparticles in the food processing industry and provides a theoretical basis for the application of daidzein nanoparticle oral delivery systems.

The invention provides a method for obtaining an extract of plant origin from an undifferentiated or dedifferentiated plant cell line by in vitro culture, the method comprising the addition of at least one aglycone flavonoid to the culture medium. The obtained extract contains a group of target secondary metabolites. It can be advantageously used for the non-therapeutic cosmetic treatment of the skin and appendages.

The invention provides a preparation method of a dogwood extract and application of the dogwood extract in bone strengthening products, and belongs to the technical field of medicine extraction. The method comprises the following steps: (1) crushing a dogwood raw material, and immediately spraying an acid solution; (2) sequentially extracting the pretreated material by adopting an ethanol water solution containing citric acid and L-ascorbic acid as a first-phase extraction solvent and a phosphate buffer solution containing a compound enzyme preparation as a second-phase extraction solvent; (3) combining the extracting solutions, sequentially separating through an ultrafiltration membrane and at least two stages of nanofiltration membranes to obtain active fractions with different molecular weight intervals, and recombining the active fractions according to a preset proportion to obtain a refined solution; and (4) drying the refined liquid to obtain the dogwood extract. The preparation method provided by the invention has the advantages of high extraction rate of target active ingredients (especially aglycone), synergistic retention of multiple active ingredients, strong process controllability, suitability for large-scale production and the like.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The invention relates to a method for extracting dihydroquercetin and arabinogalactan from larch, which is characterized by comprising the following steps: by taking larch root as a raw material, carrying out ultrasonic extraction once by using a natural surfactant assisted ethanol solution, adding ethanol into the remaining extracting solution, precipitating crude polysaccharide in the extracting solution in an alcohol precipitation manner, filtering after alcohol precipitation, and drying to obtain the dihydroquercetin and arabinogalactan. Drying the filter cake in a drying oven to obtain crude polysaccharide (containing arabinogalactan); adding an acid and an organic solvent into the filtrate, completely converting glycoside into aglycone in an acid hydrolysis manner, separating out an organic solvent phase after the reaction is finished, concentrating and directly drying the organic solvent, dissolving a solid matter with hot water, enriching with macroporous adsorption resin, eluting, and recrystallizing to obtain a product. The operation process has the main characteristics that the raw materials can be directly used without drying, the energy consumption is low, the macroporous adsorption resin purification process is pollution-free, the production efficiency is high, and the yield is high; all solvents can be recycled, so that industrial large-scale production is facilitated.

Provided is a pharmaceutical composition for preventing and treating periodontal disease, containing a plant extract of Alnus japonica as an active ingredient, and the Alnus japonica plant extract according to the present invention contains oregonin or an aglycone thereof, thereby having superior effects of alleviating, preventing, and treating periodontal diseases.

This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of fermentation of metabolically engineered microorganisms. This disclosure describes engineered micro-organisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These micro-organisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetyl glucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These micro-organisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, this disclosure provides a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and / or an aglycon, wherein the microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phosphate to N-acetylglucosamine, converts N-acetyl-glucosamine to N-acetylmannosamine and convert the latter further to N-acetyl-neuraminate.

According to the invention, beta-glycosidase which has a catalytic effect on vincoside, loganin and strictosamide is selected, and a foundation is laid for diversity and possibility of an aglycone production process. By researching the three-dimensional structure information of the Gelsemium elegans Beta-glycosidase, a zymoprotein structure information basis is provided for molecular modification of the Gelsemium elegans Beta-glycosidase, enzyme variants capable of improving substrate selectivity, thermal stability and catalytic efficiency are obtained more efficiently, and the requirements of industrial application are met; the invention provides a truncated mutant enzyme with improved enzymatic activity, and through induced expression and activity screening, it is found that the enzyme activity of the obtained mutant enzyme to loganin and strictosamide is about two times higher than that of a wild enzyme.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

The invention relates to an antibody drug conjugate of a cardiac glycoside and / or cardiac aglycone compound as shown in a formula (I), a preparation method of the antibody drug conjugate and application of the antibody drug conjugate in prevention and / or treatment of diseases related to abnormal cell activity, including but not limited to prevention and / or treatment of tumor diseases. # imgabs0 #

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The present invention discloses a β-glucosidase mutant and its application in the hydrolysis of malonyl genistin. The present invention is based on the β-glucosidase from uncultured marine microorganisms, and is designed and modified through homology modeling and molecular docking to obtain a mutant enzyme with improved efficiency and stability in hydrolyzing malonyl genistin. Under the conditions of the same mass of enzyme protein, the ratio of the mutant enzyme to hydrolyze malonyl genistin is 1.5 times higher than that of the starting enzyme; under the conditions of 30-45°C, the half-life of the mutant enzyme is 3.7 times higher than that of the starting enzyme. The mutant has potential application value in the hydrolysis of soybean isoflavones to produce aglycones.

The application discloses application of arctigenin derivative or a pharmaceutically acceptable salt thereof in preparation of a medicament for treating peripheral neuropathy in diabetes. The application shows through a large number of experiments that the arctigenin derivative and the pharmaceutically acceptable salt thereof can obviously improve nerve conduction velocity and sensory loss symptoms of diabetic mice, thereby achieving the effect of treating peripheral neuropathy.

The application belongs to the technical field of organic synthesis, and particularly relates to a benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative, a preparation method and application thereof. The benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative provided by the application has a structure shown in formula 1 or formula 2. The application combines an anemarrhena saponin aglycone skeleton and a benzimidazole skeleton, and the obtained benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative with the structure shown in formula 1 or formula 2 has significant antitumor activity. The antitumor growth inhibition activity of the benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative provided by the application is better than that of cisplatin (DDP), an anticancer drug.

The application discloses application of Hungatella strain in biological transformation of flavone carbon glycoside. The application provides a method for preparing aglycone or aglycone derivative, and comprises the following steps: taking glycoside or plant material containing glycoside as raw material, and adopting Hungatella strain or culture of Hungatella strain to perform biological transformation, so as to obtain aglycone or aglycone derivative. The method provided by the application is a microbial transformation method, and has the advantages of fast reaction rate, high product conversion rate, mild reaction condition, environmental friendliness, simple transformation process, short cycle, low cost and the like. The application is expected to efficiently hydrolyze carbon glycoside bond. The application has great application and promotion value in the field of production of aglycone or aglycone derivative and downstream products thereof.

The invention belongs to the field of medicines, and relates to a scutellarin aglycone derivative as well as a preparation method and application thereof. The scutellarin aglycone derivative has a structure as shown in a formula I. The preparation method comprises the following steps: reacting scutellarin (a compound II) with methanol to obtain a compound III, then reacting with bornyl chloroacetate (a compound IV) to obtain a compound V, and finally carrying out an alkaline hydrolysis reaction to obtain the compound I, the scutellarin aglycone derivative has the effect of preventing or treating cardiovascular and cerebrovascular related diseases, can improve the activity of superoxide dismutase (SOD), inhibit the production of lactic dehydrogenase (LDH) and reduce the malondialdehyde (MDA) level superior to that of scutellarin, has the inhibiting effect on inflammatory factors TNF-alpha, IL-6 and IL-1beta in cell supernatant superior to that of scutellarin, and can be used for preparing a medicine for treating cardiovascular and cerebrovascular related diseases, such as cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases and cardiovascular and cerebrovascular related diseases. The invention has the potential of preparing or preventing cardiovascular and cerebrovascular related diseases.

The present application relates to a preparation method and application of Dendrobium candidum extract, and belongs to the technical field of plant extraction. The present application realizes physical disruption of cell structure by using ultra-low temperature crushing technology, and avoids degradation of heat-sensitive components. A complex system of cellulase, pectinase and neutral protease is used for synergistic enzymolysis at 35-45 DEG C and pH 5-6, which efficiently hydrolyzes cell wall components and releases bound anti-inflammatory active ingredients. Subsequently, Ruminococcus lactaris is used for anaerobic fermentation, and the beta-glucosidase secreted by the bacteria hydrolyzes the bibenzyl aglycone, the metabolic product lactic acid promotes the protonation of alkaloids to form water-soluble salts, and the exopolysaccharide forms a complex active ingredient with the dendrobium polysaccharide; the fermentation broth is filtered, and is compounded with the ultrafiltration purified water extract obtained by ultrasonic assisted extraction at a ratio of (2-4):1, and finally high-purity extract is obtained by freeze-drying. The extract can be released controllably in the gastrointestinal tract, and the inhibition rate of LPS-induced inflammation is better than that of traditional processes, and can be used in the field of intestinal anti-inflammatory.

The present invention relates to a method for preparing extracellular vesicles of Lactobacillus cells with increased aglycone isoflavone content, and a composition for preventing hair loss using same. The Lactobacillus of the present invention enables the effective extraction of aglycone isoflavones contained in legumes through a fermentation process, and the extracellular vesicles secreted from the Lactobacillus exhibit anti-inflammatory and immunomodulatory properties and can be expected to have the effects of promoting scalp health and preventing hair loss. Owing to their small size and excellent intercellular delivery capability, the extracellular vesicles can act effectively in deep layers of the skin and scalp. In addition, the extracellular vesicles have high biocompatibility and fewer side effects, and can be used more safely than conventional chemical compositions.

The application discloses a hydroxylase and application thereof in synthesis of 14-deoxoandrographolide. The hydroxylase is protein CYP72A399, and the amino acid sequence of the protein CYP72A399 is shown as SEQ ID No: 2. Experiments prove that the protein CYP72A399 can be used as a hydroxylase to catalyze new andrographolide aglycone to generate 14-deoxoandrographolide. Therefore, the protein CYP72A399 has important theoretical and practical significance for synthesis of 14-deoxoandrographolide and cultivation of high-quality andrographis paniculata. The application has important application value.

The present invention belongs to the technical field of extraction, separation and purification of plant components, and specifically relates to passion fruit megastigmane glycoside compounds, their preparation methods, and applications. The passion fruit megastigmane glycoside compounds provided by the present invention are derived from passion fruit juice and are natural plant secondary metabolites. These disaccharide compounds, formed by linking a C13 norsesquiterpene aglycone to glucose and rhamnose via an oxyglycosidic bond, represent a novel class of compounds with broad application prospects in anti-anxiety, sedative-hypnotic, or flavor-enhancing products.

To provide a plant extract containing kaempferol aglycone, and a method for producing the same.SOLUTION: The plant extract contains kaempferol aglycone in an amount of ≥ 1mg / g expressed in terms of dried weight. The method for producing the extract comprises (1) extracting a plant raw material containing the kaempferol glycoside with a solvent and (2) hydrolyzing the extract obtained in the above (1).SELECTED DRAWING: None

The invention discloses a method for performing carbon glycoside deglycosylation by using a biological method. The invention provides application of the protein combination: application in splitting C-C glucosidic bonds; application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. Application in splitting C-C glucosidic bonds; the invention also relates to an application in converting mangiferin into mangiferin aglycone. The protein combination comprises an LE1 protein, an LE2 protein and an LE3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.