111 results about "Aglycone" patented technology

The invention provides a combined fermented product of ginseng, astragalus membranaceus and donkey-hide gelatin as well as a preparation method and application of the combined fermented product, and relates to the technical field of pharmaceutical preparations. The combined leavening is obtained by fermenting ginseng, astragalus membranaceus and donkey-hide gelatin, and the mass ratio of the ginseng to the astragalus membranaceus to the donkey-hide gelatin is (1-3): (10-30): 1. The composition of ginseng, astragalus membranaceus and colla corii asini is subjected to probiotic fermentation, macromolecular saponin is converted into aglycone or secondary saponin with smaller molecules, protein is split into peptide chains with smaller molecules, polysaccharide is split into sugar chains with smaller molecules, absorption and utilization of effective components of the medicine are facilitated, and the effect of tonifying qi and blood can be rapidly achieved.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a dammarane sapogenin-isatin derivative and application thereof in anti-tumor treatment. The dammarane sapogenin-isatin conjugate is constructed by taking dammarane sapogenin as a mother nucleus, deriving the dammarane sapogenin and chloroacetic acid and then introducing an indolone structural unit, and R1, R2 and R3 are as described in the claims and the specification. According to the invention, isatin fragments and ginsenoside are coupled for the first time, the anti-tumor activity of the compounds is systematically evaluated, and the action mechanism research of the compounds in colorectal cancer and other tumors is further developed. Results show that the obtained compound has relatively good anti-tumor efficacy, tumor cell selectivity, relatively low toxicity and relatively high bioavailability, and shows good patent medicine potential and wide market prospects.

The invention discloses a soybean yellow serofluid fermentation product rich in aglycone type soybean isoflavone as well as a preparation method and application of the soybean yellow serofluid fermentation product, and relates to the technical field of microbial fermentation. The preparation method comprises the following steps: inoculating double yeasts into a soybean yellow serofluid culture medium rich in glucoside type soybean isoflavone, adjusting the pH value of the soybean yellow serofluid culture medium, and performing constant-temperature fermentation to obtain a soybean yellow serofluid fermentation product; the double yeast is prepared from Wickerhamomyces anomalus NNB-1 and Candida utilis LYY1 according to a proportion, the Wickerhamomyces anomalus NNB-1 is preserved in the China Center for Type Culture Collection on April 3, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2023451. The invention further discloses a preparation method of the double yeast for the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain. According to the present invention, the soybean yellow serofluid rich in glucoside type soybean isoflavone is adopted as the fermentation substrate, and the Wickerhamomyces anomalus NNB-1 and the Candida utilis LYY1 are subjected to double-yeast fermentation so as to significantly improve the content of daidzein, daidzein, genistein and other aglycone type soybean isoflavone in the soybean yellow serofluid fermentation product.

A unit dosage of an oral composition of an extract of Withania somnifera is disclosed. The extract of Withania somnifera includes total withanolides. The total withanolides includes withanolide glycosides and withanolide aglycones. The extract of Withania somnifera includes about 32% to about 38% by weight of the withanolide glycosides. Methods of preparing the extract of Withania somnifera are disclosed. Methods of treatment by administering the extract of Withania somnifera are disclosed.

The invention discloses a method for preparing a radix pseudostellariae extract through enzymolysis, and belongs to the technical field of plant effective component extraction. The method comprises the following steps: mixing radix pseudostellariae powder with deionized water which is 10 times of the weight of the radix pseudostellariae powder, and decocting for 2-4 hours to obtain filtrate and filter residues; mixing the filter residue, deionized water and combined enzyme to form slurry, and sequentially performing enzymolysis, inactivation and filtration on the slurry to obtain an extracting solution; mixing the extracting solution and the filtrate, hydrolyzing and catalyzing by glucoside hydrolase, and inactivating to obtain hydrolysate; and sequentially performing rotary evaporation and hot air drying on the hydrolysate to obtain the radix pseudostellariae extract. The radix pseudostellariae is subjected to enzymolysis wall breaking by adopting a combined enzyme, release of effective components is promoted, then filtrate and an extracting solution obtained through wall breaking are subjected to hydrolysis to promote generation of aglycone with higher activity, and finally, the radix pseudostellariae extract is obtained through drying treatment.

The invention provides a preparation method of milbemycin oxime A4, which comprises the following steps: under an acidic condition, carrying out hydrolysis disaccharide removal reaction on tenvermectin B to obtain tenvermectin B aglycone; the preparation method comprises the following steps: carrying out silicon-based protection on tenvermectin B aglycone by adopting a silicon-based protection reagent to obtain 5-hydroxyl silicon-based tenvermectin B aglycone; the preparation method comprises the following steps: brominating 5-hydroxyl silicon-based tenvermectin B aglycone by adopting a brominating reagent to obtain 13-bromine-5-hydroxyl silicon-based protected tenvermectin B aglycone; the preparation method comprises the following steps: reducing 13-bromo-5-hydroxyl silicon group protected tenvermectin B aglycone by using a reducing agent to obtain 5-hydroxyl silicon group protected milbemycins A4; the milbemycins A4 protected by the 5-hydroxyl silicon group is subjected to desilicication protection treatment, and the milbemycins A4 is obtained; oxidizing milbemycins A4 by adopting an oxidizing agent to obtain 5-ketomilbemycins A4; the preparation method comprises the following steps: reacting 5-ketomilbemycins A4 with hydroxylamine hydrochloride to obtain milbemycins A4, and efficiently preparing the active component milbemycins A4 single component by taking tenvermectin B as a raw material.

The present invention relates to a functional coffee substitute composition. The coffee substitute food composition comprising a fermented dandelion root extract, a fermented Acanthopanax seed extract, and a fermented soybean extract of the present invention possesses sensory characteristics similar to coffee, and through fermentation, the content of major plant functional components such as isoflavone aglycone, total polyphenols, flavonoids, and saponins increases, L-carnitine is newly synthesized, and since it is a novel coffee substitute food material having antioxidant effects and anti-diabetic activity, it can be utilized as a caffeine-reducing beverage, a beverage that promotes body fat metabolism, an antioxidant-enhanced beverage, and a health functional food.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention discloses lotus leaf powder as well as a preparation method and application thereof, and belongs to the technical field of deep processing of plant raw materials. The preparation method of the lotus leaf powder provided by the invention comprises the following steps: carrying out enzymolysis on pretreated fresh lotus leaves in a system containing beta-glucosidase, and then carrying out scalding and ice water leaching cooling; the obtained lotus leaf powder has bright emerald green color, and contains more active ingredients such as flavonoid aglycones and nuciferine which are beneficial to human body absorption; moreover, the content of tannin and total flavonoid glycoside in the obtained lotus leaf powder is relatively low, so that the lotus leaf powder has relatively low bitter and astringent taste, and the obtained lotus leaf powder is excellent in mouth feel; meanwhile, the obtained lotus leaf powder also has good capability of improving blood fat; the method can be widely applied to preparation of matcha-flavored food or preparation of functional preparations for improving blood fat.

This invention provides a method for preparing a daidzein nanoparticle delivery carrier, belonging to the field of food processing technology. Using zein, carrageenan, and sodium alginate as raw materials, this invention utilizes ultrasound technology to construct a novel nanoparticle delivery carrier: zein nanoparticles loaded with a carrageenan / sodium alginate bistabilized layer, thereby improving the stability and bioavailability of daidzein nanoparticles. The advantages of this invention lie in the simplicity and environmental friendliness of the antisolvent precipitation method and ultrasound technology. The prepared composite nanoparticles possess stable physicochemical properties, small particle size, relatively concentrated particle size distribution, and high bioavailability. This invention enhances the practical application value of daidzein-loaded proteoglycan nanoparticles in the food processing industry and provides a theoretical basis for the application of daidzein nanoparticle oral delivery systems.

The invention provides a method for obtaining an extract of plant origin from an undifferentiated or dedifferentiated plant cell line by in vitro culture, the method comprising the addition of at least one aglycone flavonoid to the culture medium. The obtained extract contains a group of target secondary metabolites. It can be advantageously used for the non-therapeutic cosmetic treatment of the skin and appendages.

The invention provides a preparation method of a dogwood extract and application of the dogwood extract in bone strengthening products, and belongs to the technical field of medicine extraction. The method comprises the following steps: (1) crushing a dogwood raw material, and immediately spraying an acid solution; (2) sequentially extracting the pretreated material by adopting an ethanol water solution containing citric acid and L-ascorbic acid as a first-phase extraction solvent and a phosphate buffer solution containing a compound enzyme preparation as a second-phase extraction solvent; (3) combining the extracting solutions, sequentially separating through an ultrafiltration membrane and at least two stages of nanofiltration membranes to obtain active fractions with different molecular weight intervals, and recombining the active fractions according to a preset proportion to obtain a refined solution; and (4) drying the refined liquid to obtain the dogwood extract. The preparation method provided by the invention has the advantages of high extraction rate of target active ingredients (especially aglycone), synergistic retention of multiple active ingredients, strong process controllability, suitability for large-scale production and the like.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The invention relates to a method for extracting dihydroquercetin and arabinogalactan from larch, which is characterized by comprising the following steps: by taking larch root as a raw material, carrying out ultrasonic extraction once by using a natural surfactant assisted ethanol solution, adding ethanol into the remaining extracting solution, precipitating crude polysaccharide in the extracting solution in an alcohol precipitation manner, filtering after alcohol precipitation, and drying to obtain the dihydroquercetin and arabinogalactan. Drying the filter cake in a drying oven to obtain crude polysaccharide (containing arabinogalactan); adding an acid and an organic solvent into the filtrate, completely converting glycoside into aglycone in an acid hydrolysis manner, separating out an organic solvent phase after the reaction is finished, concentrating and directly drying the organic solvent, dissolving a solid matter with hot water, enriching with macroporous adsorption resin, eluting, and recrystallizing to obtain a product. The operation process has the main characteristics that the raw materials can be directly used without drying, the energy consumption is low, the macroporous adsorption resin purification process is pollution-free, the production efficiency is high, and the yield is high; all solvents can be recycled, so that industrial large-scale production is facilitated.

Provided is a pharmaceutical composition for preventing and treating periodontal disease, containing a plant extract of Alnus japonica as an active ingredient, and the Alnus japonica plant extract according to the present invention contains oregonin or an aglycone thereof, thereby having superior effects of alleviating, preventing, and treating periodontal diseases.

The present invention relates to a novel process for the preparation of gliflozin aglycone through a telescopic approach using ortho-lithiation of 1,4-dihalobenzene followed by reacting with corresponding aromatic or heteroaromatic aldehydes and reduction of the resulted in diarylmethanol or (aryl)(heteroaryl)methanol intermediate as key steps.

This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of fermentation of metabolically engineered microorganisms. This disclosure describes engineered micro-organisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These micro-organisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetyl glucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These micro-organisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, this disclosure provides a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and / or an aglycon, wherein the microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phosphate to N-acetylglucosamine, converts N-acetyl-glucosamine to N-acetylmannosamine and convert the latter further to N-acetyl-neuraminate.

The invention discloses a method for extracting isoflavone aglycone from soybean germs.The method comprises the following steps that S1, soybean germ powder obtained after superfine grinding is used as a raw material, the soybean germ powder and a weak acid buffer solution are mixed to be uniform, and a mixed solution with the soybean germ powder mass concentration being 5-15% is prepared; s2, performing ultrasonic and / or microwave-assisted solvent extraction operation on the mixed solution obtained in the step S1 to obtain an isoflavone-rich extracting solution; and S3, carrying out an enzymolysis reaction on the isoflavone-rich extracting solution obtained in the step S2 in a multi-stage biological enzyme reactor to obtain a high-purity isoflavone aglycone product. According to the extraction method of the isoflavone aglycone, the biological enzyme is high in enzyme activity and can be repeatedly used, and the obtained isoflavone aglycone is high in extraction rate and purity.

According to the invention, beta-glycosidase which has a catalytic effect on vincoside, loganin and strictosamide is selected, and a foundation is laid for diversity and possibility of an aglycone production process. By researching the three-dimensional structure information of the Gelsemium elegans Beta-glycosidase, a zymoprotein structure information basis is provided for molecular modification of the Gelsemium elegans Beta-glycosidase, enzyme variants capable of improving substrate selectivity, thermal stability and catalytic efficiency are obtained more efficiently, and the requirements of industrial application are met; the invention provides a truncated mutant enzyme with improved enzymatic activity, and through induced expression and activity screening, it is found that the enzyme activity of the obtained mutant enzyme to loganin and strictosamide is about two times higher than that of a wild enzyme.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

The invention relates to an antibody drug conjugate of a cardiac glycoside and / or cardiac aglycone compound as shown in a formula (I), a preparation method of the antibody drug conjugate and application of the antibody drug conjugate in prevention and / or treatment of diseases related to abnormal cell activity, including but not limited to prevention and / or treatment of tumor diseases. # imgabs0 #

The preparation method comprises the following steps: mixing soybean whey with rose residue powder, inoculating a mixed seed solution of lactobacillus plantarum SJ-L-1 and saccharomyces cerevisiae, and fermenting to prepare a physalis alkekengi stock solution. According to the invention, lactobacillus plantarum SJ-L-1 and saccharomyces cerevisiae are utilized, soybean whey is used as a fermentation substrate, and rose residue powder is used as an auxiliary agent to prepare the sour pulp beverage. The obtained physalis alkekengi beverage has bright rose red color, not only retains the color, fragrance and taste of roses, but also eliminates the peculiar smell of soybean whey, meanwhile, ester fragrance and mellow fragrance are generated in the fermentation process, the flavor is rich, the taste is sour and refreshing, in addition, glucoside type isoflavone is converted into aglycone type isoflavone to the greatest extent, and the nutritional value of the rose physalis alkekengi beverage is improved. The method is simple in technological process, easy and convenient to operate, low in cost and easy to industrialize.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The present invention discloses a β-glucosidase mutant and its application in the hydrolysis of malonyl genistin. The present invention is based on the β-glucosidase from uncultured marine microorganisms, and is designed and modified through homology modeling and molecular docking to obtain a mutant enzyme with improved efficiency and stability in hydrolyzing malonyl genistin. Under the conditions of the same mass of enzyme protein, the ratio of the mutant enzyme to hydrolyze malonyl genistin is 1.5 times higher than that of the starting enzyme; under the conditions of 30-45°C, the half-life of the mutant enzyme is 3.7 times higher than that of the starting enzyme. The mutant has potential application value in the hydrolysis of soybean isoflavones to produce aglycones.

To provide a conjugate for transferring an effector molecule from outside a cell into said cell.SOLUTION: Provided is a conjugate comprising an effector molecule, a cell surface molecule targeting antibody, and a saponin. Therein: the effector molecule, the antibody, and the saponin are covalently bound to each other; the effector molecule is an oligonucleotide; the saponin is a bi-desmosidic triterpene glycoside comprising an aglycone core structure selected from quillaic acid or gypsogenin and comprising a first glycan bound to a C3 atom of the aglycone core structure and a second glycan bound to a C28 atom of the aglycone core structure; the first glycan is branched and comprising a glucuronic acid unit; the second glycan is branched and comprising a carbohydrate unit selected from any one or more of fucose (Fuc), rhamnose (Rha), and quinovose (Qui); and the antibody is capable of binding to a cell surface molecule of the cell.SELECTED DRAWING: None

The application discloses application of arctigenin derivative or a pharmaceutically acceptable salt thereof in preparation of a medicament for treating peripheral neuropathy in diabetes. The application shows through a large number of experiments that the arctigenin derivative and the pharmaceutically acceptable salt thereof can obviously improve nerve conduction velocity and sensory loss symptoms of diabetic mice, thereby achieving the effect of treating peripheral neuropathy.

The invention discloses a production strain of demethylated ristomycin aglycone as well as a construction method and application of the production strain. The construction method comprises the following steps: synthesizing a gene cluster lacking a glycosylation modification function by adopting a gene editing mode, and then expressing the edited gene cluster in a heterologous or homologous host; or the glycosyl transferase gene in the strain containing the ristomycin biosynthetic gene cluster is knocked out. At present, no report about biosynthesis of the demethylated ristomycin aglycone exists, and the invention provides a biosynthesis method of the demethylated ristomycin aglycone. Meanwhile, the demethylated ristomycin aglycone production strain provided by the invention has good genetic operability, is fast in growth and short in fermentation time, can reach the peak of producing the ristomycin aglycone after being fermented for 2-3 days, and has excellent industrial development potential.

The application belongs to the technical field of organic synthesis, and particularly relates to a benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative, a preparation method and application thereof. The benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative provided by the application has a structure shown in formula 1 or formula 2. The application combines an anemarrhena saponin aglycone skeleton and a benzimidazole skeleton, and the obtained benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative with the structure shown in formula 1 or formula 2 has significant antitumor activity. The antitumor growth inhibition activity of the benzimidazole alkyl bromide salt-anemarrhena saponin aglycone derivative provided by the application is better than that of cisplatin (DDP), an anticancer drug.

The application discloses application of Hungatella strain in biological transformation of flavone carbon glycoside. The application provides a method for preparing aglycone or aglycone derivative, and comprises the following steps: taking glycoside or plant material containing glycoside as raw material, and adopting Hungatella strain or culture of Hungatella strain to perform biological transformation, so as to obtain aglycone or aglycone derivative. The method provided by the application is a microbial transformation method, and has the advantages of fast reaction rate, high product conversion rate, mild reaction condition, environmental friendliness, simple transformation process, short cycle, low cost and the like. The application is expected to efficiently hydrolyze carbon glycoside bond. The application has great application and promotion value in the field of production of aglycone or aglycone derivative and downstream products thereof.