64 results about "Aglycone" patented technology

A unit dosage of an oral composition of an extract of Withania somnifera is disclosed. The extract of Withania somnifera includes total withanolides. The total withanolides includes withanolide glycosides and withanolide aglycones. The extract of Withania somnifera includes about 32% to about 38% by weight of the withanolide glycosides. Methods of preparing the extract of Withania somnifera are disclosed. Methods of treatment by administering the extract of Withania somnifera are disclosed.

The invention provides a preparation method of milbemycin oxime A4, which comprises the following steps: under an acidic condition, carrying out hydrolysis disaccharide removal reaction on tenvermectin B to obtain tenvermectin B aglycone; the preparation method comprises the following steps: carrying out silicon-based protection on tenvermectin B aglycone by adopting a silicon-based protection reagent to obtain 5-hydroxyl silicon-based tenvermectin B aglycone; the preparation method comprises the following steps: brominating 5-hydroxyl silicon-based tenvermectin B aglycone by adopting a brominating reagent to obtain 13-bromine-5-hydroxyl silicon-based protected tenvermectin B aglycone; the preparation method comprises the following steps: reducing 13-bromo-5-hydroxyl silicon group protected tenvermectin B aglycone by using a reducing agent to obtain 5-hydroxyl silicon group protected milbemycins A4; the milbemycins A4 protected by the 5-hydroxyl silicon group is subjected to desilicication protection treatment, and the milbemycins A4 is obtained; oxidizing milbemycins A4 by adopting an oxidizing agent to obtain 5-ketomilbemycins A4; the preparation method comprises the following steps: reacting 5-ketomilbemycins A4 with hydroxylamine hydrochloride to obtain milbemycins A4, and efficiently preparing the active component milbemycins A4 single component by taking tenvermectin B as a raw material.

The invention discloses lotus leaf powder as well as a preparation method and application thereof, and belongs to the technical field of deep processing of plant raw materials. The preparation method of the lotus leaf powder provided by the invention comprises the following steps: carrying out enzymolysis on pretreated fresh lotus leaves in a system containing beta-glucosidase, and then carrying out scalding and ice water leaching cooling; the obtained lotus leaf powder has bright emerald green color, and contains more active ingredients such as flavonoid aglycones and nuciferine which are beneficial to human body absorption; moreover, the content of tannin and total flavonoid glycoside in the obtained lotus leaf powder is relatively low, so that the lotus leaf powder has relatively low bitter and astringent taste, and the obtained lotus leaf powder is excellent in mouth feel; meanwhile, the obtained lotus leaf powder also has good capability of improving blood fat; the method can be widely applied to preparation of matcha-flavored food or preparation of functional preparations for improving blood fat.

This invention provides a method for preparing a daidzein nanoparticle delivery carrier, belonging to the field of food processing technology. Using zein, carrageenan, and sodium alginate as raw materials, this invention utilizes ultrasound technology to construct a novel nanoparticle delivery carrier: zein nanoparticles loaded with a carrageenan / sodium alginate bistabilized layer, thereby improving the stability and bioavailability of daidzein nanoparticles. The advantages of this invention lie in the simplicity and environmental friendliness of the antisolvent precipitation method and ultrasound technology. The prepared composite nanoparticles possess stable physicochemical properties, small particle size, relatively concentrated particle size distribution, and high bioavailability. This invention enhances the practical application value of daidzein-loaded proteoglycan nanoparticles in the food processing industry and provides a theoretical basis for the application of daidzein nanoparticle oral delivery systems.

The invention provides a preparation method of a dogwood extract and application of the dogwood extract in bone strengthening products, and belongs to the technical field of medicine extraction. The method comprises the following steps: (1) crushing a dogwood raw material, and immediately spraying an acid solution; (2) sequentially extracting the pretreated material by adopting an ethanol water solution containing citric acid and L-ascorbic acid as a first-phase extraction solvent and a phosphate buffer solution containing a compound enzyme preparation as a second-phase extraction solvent; (3) combining the extracting solutions, sequentially separating through an ultrafiltration membrane and at least two stages of nanofiltration membranes to obtain active fractions with different molecular weight intervals, and recombining the active fractions according to a preset proportion to obtain a refined solution; and (4) drying the refined liquid to obtain the dogwood extract. The preparation method provided by the invention has the advantages of high extraction rate of target active ingredients (especially aglycone), synergistic retention of multiple active ingredients, strong process controllability, suitability for large-scale production and the like.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

Provided is a pharmaceutical composition for preventing and treating periodontal disease, containing a plant extract of Alnus japonica as an active ingredient, and the Alnus japonica plant extract according to the present invention contains oregonin or an aglycone thereof, thereby having superior effects of alleviating, preventing, and treating periodontal diseases.

According to the invention, beta-glycosidase which has a catalytic effect on vincoside, loganin and strictosamide is selected, and a foundation is laid for diversity and possibility of an aglycone production process. By researching the three-dimensional structure information of the Gelsemium elegans Beta-glycosidase, a zymoprotein structure information basis is provided for molecular modification of the Gelsemium elegans Beta-glycosidase, enzyme variants capable of improving substrate selectivity, thermal stability and catalytic efficiency are obtained more efficiently, and the requirements of industrial application are met; the invention provides a truncated mutant enzyme with improved enzymatic activity, and through induced expression and activity screening, it is found that the enzyme activity of the obtained mutant enzyme to loganin and strictosamide is about two times higher than that of a wild enzyme.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The application discloses application of Hungatella strain in biological transformation of flavone carbon glycoside. The application provides a method for preparing aglycone or aglycone derivative, and comprises the following steps: taking glycoside or plant material containing glycoside as raw material, and adopting Hungatella strain or culture of Hungatella strain to perform biological transformation, so as to obtain aglycone or aglycone derivative. The method provided by the application is a microbial transformation method, and has the advantages of fast reaction rate, high product conversion rate, mild reaction condition, environmental friendliness, simple transformation process, short cycle, low cost and the like. The application is expected to efficiently hydrolyze carbon glycoside bond. The application has great application and promotion value in the field of production of aglycone or aglycone derivative and downstream products thereof.

The invention belongs to the field of medicines, and relates to a scutellarin aglycone derivative as well as a preparation method and application thereof. The scutellarin aglycone derivative has a structure as shown in a formula I. The preparation method comprises the following steps: reacting scutellarin (a compound II) with methanol to obtain a compound III, then reacting with bornyl chloroacetate (a compound IV) to obtain a compound V, and finally carrying out an alkaline hydrolysis reaction to obtain the compound I, the scutellarin aglycone derivative has the effect of preventing or treating cardiovascular and cerebrovascular related diseases, can improve the activity of superoxide dismutase (SOD), inhibit the production of lactic dehydrogenase (LDH) and reduce the malondialdehyde (MDA) level superior to that of scutellarin, has the inhibiting effect on inflammatory factors TNF-alpha, IL-6 and IL-1beta in cell supernatant superior to that of scutellarin, and can be used for preparing a medicine for treating cardiovascular and cerebrovascular related diseases, such as cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases and cardiovascular and cerebrovascular related diseases. The invention has the potential of preparing or preventing cardiovascular and cerebrovascular related diseases.

The present application relates to a preparation method and application of Dendrobium candidum extract, and belongs to the technical field of plant extraction. The present application realizes physical disruption of cell structure by using ultra-low temperature crushing technology, and avoids degradation of heat-sensitive components. A complex system of cellulase, pectinase and neutral protease is used for synergistic enzymolysis at 35-45 DEG C and pH 5-6, which efficiently hydrolyzes cell wall components and releases bound anti-inflammatory active ingredients. Subsequently, Ruminococcus lactaris is used for anaerobic fermentation, and the beta-glucosidase secreted by the bacteria hydrolyzes the bibenzyl aglycone, the metabolic product lactic acid promotes the protonation of alkaloids to form water-soluble salts, and the exopolysaccharide forms a complex active ingredient with the dendrobium polysaccharide; the fermentation broth is filtered, and is compounded with the ultrafiltration purified water extract obtained by ultrasonic assisted extraction at a ratio of (2-4):1, and finally high-purity extract is obtained by freeze-drying. The extract can be released controllably in the gastrointestinal tract, and the inhibition rate of LPS-induced inflammation is better than that of traditional processes, and can be used in the field of intestinal anti-inflammatory.

The present invention relates to a method for preparing extracellular vesicles of Lactobacillus cells with increased aglycone isoflavone content, and a composition for preventing hair loss using same. The Lactobacillus of the present invention enables the effective extraction of aglycone isoflavones contained in legumes through a fermentation process, and the extracellular vesicles secreted from the Lactobacillus exhibit anti-inflammatory and immunomodulatory properties and can be expected to have the effects of promoting scalp health and preventing hair loss. Owing to their small size and excellent intercellular delivery capability, the extracellular vesicles can act effectively in deep layers of the skin and scalp. In addition, the extracellular vesicles have high biocompatibility and fewer side effects, and can be used more safely than conventional chemical compositions.

The application discloses a hydroxylase and application thereof in synthesis of 14-deoxoandrographolide. The hydroxylase is protein CYP72A399, and the amino acid sequence of the protein CYP72A399 is shown as SEQ ID No: 2. Experiments prove that the protein CYP72A399 can be used as a hydroxylase to catalyze new andrographolide aglycone to generate 14-deoxoandrographolide. Therefore, the protein CYP72A399 has important theoretical and practical significance for synthesis of 14-deoxoandrographolide and cultivation of high-quality andrographis paniculata. The application has important application value.

To provide a plant extract containing kaempferol aglycone, and a method for producing the same.SOLUTION: The plant extract contains kaempferol aglycone in an amount of ≥ 1mg / g expressed in terms of dried weight. The method for producing the extract comprises (1) extracting a plant raw material containing the kaempferol glycoside with a solvent and (2) hydrolyzing the extract obtained in the above (1).SELECTED DRAWING: None

The invention discloses a method for performing carbon glycoside deglycosylation by using a biological method. The invention provides application of the protein combination: application in splitting C-C glucosidic bonds; application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. Application in splitting C-C glucosidic bonds; the invention also relates to an application in converting mangiferin into mangiferin aglycone. The protein combination comprises an LE1 protein, an LE2 protein and an LE3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.

The invention relates to the fields of therapy and drug delivery. It is based on an unexpected finding that a combination of an oligonucleotide-based medicament with a saponin component comprising a penta-cyclic triterpene saponin of a 12,13-dehydrooleanane aglycone core, not only does not appear to be associated with oligonucleotide medicament-induced nephrotoxic effects after systemic administration in vivo, but also that it allows the oligonucleotide-based medicament to enter and act on its nucleic acid target inside of the kidney cells instead of remaining unproductively trapped in renal subcellular compartments or being eliminated into the urine via the renal glomerular filtration system. In line with this finding, herein disclosed are therapeutic combinations, compositions, and formulations, as well as methods of treatment involving their administration, which comprise an oligonucleotide-based medicament that acts on an intracellular nucleic acid target in the kidney cells and / or in the cells of the spleen, being the other blood filtering organ in addition to the kidney, and a saponin component that enables the effective release of said medicament inside of these cells, thus allowing its use at a much lower and safer dose. The provision of the presented herein therapeutic combinations enables effective modulation of gene expression in the kidney and / or spleen cells and, therefore, it opens not only new treatment options for kidney diseases, in particular those involving inflammation and / or splenomegaly, but also for diseases of other organs which affect or are affected by the pathological processes happening in the kidneys and / or in the spleen, in particular being inflammatory processes.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The invention discloses a high-yield beta-glucosidase strain and application of the high-yield beta-glucosidase strain in efficient conversion of genistin, and belongs to the technical field of microbial fermentation and enzyme engineering. In order to solve the problems of low enzyme activity and low conversion efficiency of a beta-glucosidase production strain in the prior art, the invention provides the lactobacillus mucus DQ7-1 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36230, and the lactobacillus mucus DQ7-1 has the advantages that the lactobacillus mucus DQ7-1 has the preservation number of CGMCC No.36230; the strain is obtained through natural fermentation with soybean whey as a raw material in combination with aesculin color development and p-nitrobenzene-beta-D-glucopyranoside quantitative enzyme activity screening methods, and the beta-glucosaccharase activity of the strain is remarkably higher than that of common lactic acid bacteria. The strain is mainly used for catalyzing efficient conversion of soybean isoflavone glycoside, especially genistin, to genistein in the aglycone form, and has important application value in the fields of functional food, medicine and feed.

The invention discloses a method for extracting hypoglycemic active components of origanum vulgare through combination of enzymolysis, ultrasonic treatment and salting-out. According to the method, origanum vulgare raw materials are used as substrates, cell walls are destroyed through enzymolysis pretreatment, and the release efficiency of active ingredients is improved; extracting hypoglycemic active substances such as luteolin and the like by utilizing ultrasonic strengthening; primary purification is realized by adopting a salting-out mode; the content of aglycone is increased through acidic hydrolysis, and then multi-stage separation and purification are carried out to obtain high-purity active ingredients; and finally, the stability of the extract is improved by using a beta-cyclodextrin inclusion technology. The method is short in technological process, high in efficiency, capable of obtaining the high-activity extract with the luteolin content larger than or equal to 30%, and suitable for research and development of functional food, health care products and drugs.

The application discloses a method for improving aglycone conversion efficiency by using a eutectic solvent and belongs to the technical field of green extraction. The method for improving aglycone conversion efficiency by using a eutectic solvent is characterized by comprising the following steps: uniformly mixing kaempferol-3-O-rutinoside, a eutectic solvent solution and a rutin degrading enzyme solution, performing an enzymolysis reaction and obtaining an enzymolysis solution; then, purifying the enzymolysis solution to obtain aglycone kaempferol; wherein the eutectic solvent in the eutectic solvent solution is one of betaine-ethylene glycol, choline chloride-urea, choline chloride-malic acid, choline chloride-urea-acetamide. The method is green and efficient, the prepared aglycone kaempferol has a high yield of more than 45% and a high purity of more than 95%.

This invention discloses a method for determining the flavonoid aglycone content in acid-hydrolyzed sea buckthorn leaf extract, belonging to the field of detection technology. The invention involves acid-hydrolyzing sea buckthorn leaf extract to obtain a filtrate containing flavonoid aglycones, which is used as the test solution. High-performance liquid chromatography (HPLC) is used to detect the flavonoid aglycone content in both the test solution and the reference solution. The preparation method of the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin includes: adding sea buckthorn leaf extract to methanol and hydrochloric acid solutions, mixing thoroughly, heating under reflux, cooling, shaking, and filtering to obtain the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin. This invention simplifies the diverse chemical structures of flavonoid glycosides to a few flavonoid aglycones, effectively reducing dependence on expensive and scarce glycoside reference standards, simplifying chromatographic separation, and improving the stability and reproducibility of the method.

The invention relates to an antibacterial composition capable of balancing micro-ecology and a preparation method thereof, and the antibacterial composition is prepared by taking platycodon grandiflorum and iris tectorum roots as raw materials through fermentation and extraction. The content of polysaccharide and flavonoid components in the traditional Chinese medicine composition is increased through a fermentation technology, and enzyme generated by microorganisms in the fermentation process can hydrolyze macromolecular polysaccharide and generate micromolecular oligosaccharide, so that the prebiotic effect of the traditional Chinese medicine composition is improved; in plants, flavone can be combined with glucoside to form flavonoid glycoside, and the fermentation process can promote hydrolysis of glucosidic bonds, release flavonoid aglycones and improve the antibacterial effect.

The invention provides a traditional Chinese medicine composition, a preparation method thereof and application of the traditional Chinese medicine composition in preparation of hypoglycemic products, and relates to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials: 4-8 parts of ginseng stem and leaf saponin, 8-16 parts of astragalus polysaccharide, 6-12 parts of astragaloside, 8-16 parts of catalpol, 6-12 parts of rehmannia D, 8-16 parts of verbascoside, 3-5 parts of raspberry polysaccharide, 4-8 parts of ellagic acid, 4-8 parts of pachymaran, 5-10 parts of pachymic acid, 8-16 parts of lycium barbarum polysaccharide, 40-62 parts of schisandra chinensis, 20-40 parts of Chinese yam, 40-62 parts of radix ophiopogonis, 40-62 parts of radix trichosanthis and 40-62 parts of rhizoma alismatis. The traditional Chinese medicine raw materials are pretreated by using the high-voltage pulsed electric field, and then ethanol extraction treatment is performed twice, so that the yield of the traditional Chinese medicine extract is high, and the traditional Chinese medicine composition obtained by mixing the traditional Chinese medicine extract with other active ingredients has an excellent effect of treating type II diabetes mellitus.

Provided is a targeted nano-delivery kit comprising an active ingredient, a targeted guiding agent, and an optional delivery carrier and / or polymer, wherein the targeted guiding agent has the following characteristics: (1) it possesses a recognition group in its chemical structure; (2) the recognition group contains one or more of glycosyl groups of n-valent rhamnose, mannose, and galactose, and derivatives thereof; and (3) the recognition group is linked to an aglycone and / or other glycosyl groups of the targeted guiding agent by means of a covalent bond, where 1 ≤ n ≤ 10. The targeted nano-delivery kit has high drug loading and encapsulation efficiency, exhibits no intrinsic cytotoxicity, and can achieve multiple targeted deliveries. It improves the reliability of the delivery system while providing differentiated pharmacokinetic characteristics, reduces the systemic toxicity of undifferentiated system administration in the prior art, reduces first-pass metabolism, and thereby provides a safer, more reliable, and more effective treatment method.

The invention relates to the technical field of deep processing of tea leaves, and discloses a preparation process of medicinal and edible fermented tea, which sequentially comprises the steps of tea base prefabrication, medicine source enzymatic hydrolysate preparation, dietary fiber auxiliary material recovery, inoculated fermentation and gradient drying. The method is characterized in that a physical structure beneficial to microbial colonization is constructed by controlling the cell breakage rate of tea bases; a compound enzyme system containing cellulase, pectinase and beta-glucosidase is adopted for carrying out directional hydrolysis on raw materials such as rhizoma polygonati and radix puerariae, bitter glycoside is converted into sweet aglycone, and an ultramicro dietary fiber auxiliary material is prepared to serve as a fermentation carrier; after the eurotium cristatum is inoculated, the uniform growth of the golden flowers and the formation of flavor substances are promoted through two-stage temperature-control and humidity-control fermentation; and finally performing gradient drying to obtain a finished product. The process effectively solves the problems of few eurotium cristatum, heavy medicine flavor and rough taste of the traditional medicinal and edible fermented tea, realizes deep fusion of fungus fragrance and medicine fragrance tea fragrance, and has the advantages of flourishing eurotium cristatum, smooth soup feeling and stable quality.

The invention belongs to the technical field of plant extracts, and particularly discloses a preparation method of a low-toxicity and high-activity red clover extract, which comprises the following steps: crushing dried whole red clover into raw material powder; adding an ethanol aqueous solution into the raw material powder, and concentrating to obtain a crude extract; adding a compound enzyme preparation into the crude extract, and carrying out enzymolysis; carrying out enzyme deactivation on the enzymatic hydrolysate, and inoculating a composite probiotic liquid; meanwhile, plant-derived exosomes are added, and fermentation is performed; and after fermentation is finished, adding a protein solution, adsorbing through macroporous resin, and concentrating and drying to obtain the red clover extract. According to the preparation method of the low-toxicity and high-activity red clover extract, efficient bioconversion from isoflavone glycoside to aglycone is realized through a synergistic process of alcohol extraction, probiotic fermentation, protein combination, enzymolysis and plant exosome loading, macromolecular impurities are removed, toxicity is reduced, and the yield of the red clover extract is increased. The plant exosome is used for improving the stability and skin permeability of active ingredients, and finally the functional raw material suitable for high-end skin care products is obtained.

The invention relates to the technical field of deep processing and modification of vegetable protein, in particular to a modified soybean whey protein product with high isoflavone aglycone content and a preparation method thereof, and the preparation method comprises the following steps: carrying out pulsed electric field treatment on soybean whey protein suspension, adjusting the pH value of a system to be alkaline, adding alkaline protease, carrying out first constant-temperature hydrolysis treatment, and carrying out second constant-temperature hydrolysis treatment; adjusting the pH value of the system to be acidic, adding beta-glucosidase, carrying out second constant-temperature hydrolysis treatment, carrying out enzyme deactivation heat treatment, and carrying out solid-liquid separation to obtain supernate; sequentially performing ultrafiltration concentration and freeze drying on the supernate to obtain a modified soybean whey protein product with high isoflavone aglycone content; the modified protein product has high isoflavone aglycone content and low bitter taste, has high emulsibility and activity, and is suitable for the fields of functional beverages and food additives.