115 results about "Apigetrin" patented technology

The invention discloses a method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC and belongs to the technical field of medicinal plant constituent analysis.The method includes the steps that firstly, a mixed standard substance solution and a vine tea test sample solution are prepared respectively, then the mixed standard substance solution and the vine tea test sample solution are detected respectively through high performance liquid chromatography, and the contents of dihydromyricetin, dihydroquercetin, aromadendrin, naringenin, hesperetin, myricetin, myricetrin, kaempferol, quercetin, rutin and apigenin in a test sample are obtained through calculation.The measuring method is good in stability and reproducibility, easy to operate, accurate and reliable in result and capable of being applied to simultaneous measurement of multiple flavonoid constituents in the vine tea.

The invention relates to the technical field of biological medicine, and discloses an NMN (Beta-Nicotinamide Mononucleotide) beneficial bacterium health composition and a preparation method and application thereof. The composition comprises in parts by weight: 20 to 40 parts of NMN, 10 to 15 parts of beneficial bacterium powder, 0 to 8 parts of betaine, 0 to 5 parts of grape seed extract, 0 to 5 parts of baicalein, 0 to 5 parts of apigenin, 0 to 8 parts of astaxanthin, 0 to 8 parts of ginsenoside, and 0 to 8 parts of ganoderma lucidum polysaccharide (GLP); beneficial bacteria in the NMN beneficial bacterium health composition prepared by the invention can effectively improve the microecology of intestinal floras of a user, not only can improve the metabolic syndrome caused by the intestinal floras of the user, but also can improve the intestinal absorption efficiency, and is beneficial to the absorption of the NMN in the intestinal tract; the ginsenoside and the GLP are mainly matchedto improve the immunity of the user, so that the user can better adapt to various changes of the metabolic pathway level, which are caused by the case that the NAD + level is improved after the NMN enters the human body, and the organism has higher buffering capacity on the changes; and AMPK activators (the betaine, the baicalein and the apigenin) have a synergistic effect with the NMN, so that the NMN beneficial bacterium health composition is better in effect and has no side effects.

The invention discloses a preparation method of sephadex surface apigenin molecular engram sorbing material as well as the application of the sephadex surface apigenin molecular engram sorbing material to selective adsorption separation of apigenin molecules in the analysis of foods and medicine. In the method, sephadex is taken as a support, and apigenin molecular engram polymer is decorated on the surface of the sephadex. The invention is characterized in that apigenin, acrylamide, ethylene-glycol dimethyl acrylate, azodiisobutyronitrile and acylation sephadex are added in a certain proportion; and in the medium of furanidine, argon gas is used to remove oxygen, an reaction lasts for 20 to 30 h in thermostatic water bath at the temperature of 60 to 65 DEG C, and then filtering and washing are performed. Acetic acid and methanol solution with the volume fraction of 12 to 18 percent is used for Soxhlet extraction for 12 to 22 h so as to remove apigenin template molecules, and then the material is obtained through washing and drying. The invention has the advantages that a specific recognition capability to apigenin molecules is achieved, the selectivity is high, the adsorbing speed is fast, the adsorbing performance is excellent, the biological degradation is achieved, the process is simple, and regeneration capability and environmental protection are achieved.

The invention relates to the technical field of natural medicinal chemistry and food medicine, in particular to a celery seed extract and a preparation method and an application thereof. The celery seed extract is prepared from apigenin and apigenin, and the preparation method comprises the following steps: smashing celery seeds into celery seed powder; extracting and removing volatile oil compounds in celery seeds by using an organic solvent, extracting the celery seeds by using an ethanol-water solution, concentrating, and carrying out column chromatography to obtain the celery seed extract. The precise active ingredient in celery seed for lowering blood uric acid is obtained. The method is simple to operate, clear in extract effect and simple in preparation process, and the obtained celery seed extract is clear in component and can be used for health food or preventive drugs or nourishment to prevent and treat various diseases of human bodies, such as reduction of blood uric acid, relief and treatment of ventilation, prevention of gout and the like.

The invention discloses a preparation method of apigenin-7-O-beta-D-glucuronide, and a use of of apigenin-7-O-beta-D-glucuronide, and belongs to the fields of extraction and application of effective components of traditional Chinese medicines. The method comprises the steps of extraction, water precipitation and purification. The method is simple and convenient, and the prepared apigenin-7-O-beta-D-glucuronide has a high purity, and has a good curative effect on the coronary heart disease and myocardial infarction.

The invention discloses a pteris multifida extract which is extracted from pteris plants. The pteris multifida extract mainly contains flavonoid ingredients including apigenin-7-O-beta-D-glucose-4'-O-alpha-L-rhamnoside, luteolin-7-O-beta-D-glucoside and the like. The invention also discloses a preparation method of the pteris multifida extract, mainly characterized in that the extract is obtained through processes of ethanol extraction, water precipitation treatment, macroporous resin column purification and the like performed on the pteris plants, wherein the macroporous resin preferably is macroporous resin of weak polarity. The invention further discloses uses of the pteris multifida extract in the fields of medicine and food. The pteris multifida extract is mainly characterized by anti-inflammatory effect, especially anti-prostatitis effect or benign prostatic hyperplasia inhibition effect.

The invention relates to a bacterial strain for preparing glucosyl group apigenin by glycosylation in a nonaqueous phase, which is Staphylococcus saprophyticus CQ16 with a collection number of CCTCC (China Center For Type Culture Collection) NO: M2012099. The invention also provides an application of the bacterial strain in preparing the glucosyl group apigenin and a method for preparing the glucosyl group apigenin by the glycosylation in the nonaqueous phase by the bacterial strain. According to the bacterial strain separated with the method disclosed by the invention, the apigenin can be efficiently catalyzed to prepare the glucosyl group apigenin. Meanwhile, the insoluble substrate apigenin can generate glycosylation reaction with a glucose donor in the presence of biological catalyst under relatively high concentration in the nonaqueous phase, reaction catalytic efficiency is improved, the problem that apigenin glucoside compound is scarce is solved, the solubility is improved, and a foundation is laid for further researching the biological activity of flavonoid compounds.

The invention provides flavone synthase and application thereof. The flavone synthase is selected from (a) polypeptide with an amino acid sequence shown in SEQ ID NO:1 or SEQ ID NO:2, or (b) polypeptide formed by substitution, deletion, or addition of one or more amino acid residues of the amino acid sequence in SEQ ID NO:1 or SEQ ID NO:2, with the activity of the flavone synthase, and derived from (a), or (c) polypeptide with a sequence at least 85% identical to the amino acid sequence in SEQ ID NO:1 or SEQ ID NO:2 and the activity of the flavone synthase, and derived from (a). The flavone synthase can realize conversion and preparation of a plurality of flavone compounds, including apigenin, the better catalytic efficiency is achieved, the conversion efficiency better than that of existing FNS I is achieved, and the flavone synthase can be used for construction and optimization of flavone compound cell factories.

The invention discloses a method for preparing an amorphous compound with apigenin and apigenin powder particles prepared by the method. The method is characterized in that technologies such as anti-solvent, spray drying, high-pressure homogenization and supercritical fluid are adopted to realize the preparation of amorphous apigenin, particularly the preparation of ultrafine or nanoscale amorphous apigenin particles with controllable particle size and narrow particle size distribution. As the apigenin prepared by the method is amorphous powder with controllable particle size and narrow particle size distribution, the amorphous compound is stable to temperature, light and oxygen, and can be stored easily and be used to prepare pharmaceutical preparations conveniently. Compared with apigenin crystals, the solubility of the apigenin amorphous compound is greatly increased, thus the bioavailability can be increased.

The invention relates to a response-surface-method based enzyme extraction method and content determination of Chinese violet apigenin, and belongs to the technical field of traditional Chinese medicine. The response-surface-method based enzyme extraction method and the content determination have the advantages that the Chinese violet apigenin is extracted through cellulose enzyme extraction, optimal process parameters are acquired through response surface analysis based screening of enzymolysis temperature, enzymolysis time, enzymolysis pH value and cellulose consumption in the process of cellulose enzyme extraction, and the yield of the Chinese violet apigenin extracted according to the process parameters reaches more than 38.50microgram/gram; the process is eco-environment friendly, stable and feasible, residual solvents and solvent pollution both generated during extraction by organic solvents are avoided effectively, and safety and environment protection can be achieved while theChinese violet apigenin is extracted efficiently.

The invention discloses a method of preparing apigenin by treating adinandra nitida leaves by a steam explosion technology. The method comprises the following steps: performing steam explosion treatment on the adinandra nitida leaves, controlling the pressure in the steam explosion process to 0.5-3.0MPa and keeping the process for 0.5-2.0 minutes; then, instantly exploding to obtain the exploded adinandra nitida leaves, converting flavonoids such as camellianin A and camellianin B in the adinandra nitida leaves at a high temperature under high pressure into aglycone apigenin of the flavonoids, and obtaining the apigenin with relatively high purity by taking the treated adinandra nitida leaves as raw materials through organic solvent extraction and recrystallization. The preparation process of apigenin is high in yield, good in purity, low in energy consumption, free of acid and alkaline, less in wastewater emission, beneficial to environmental protection and suitable for industrial production.

The invention discloses a method for preparing apigenin by utilizing biological enzymolysis. The method comprises the following steps of: S1, enzymolysis: adding rhoifolin and water or a lower alcohol solution into a reaction container, uniformly mixing, heating to 70 DEG C or above, preserving heat, cooling to 60 DEG C or below, adding acid to adjust the pH value, adding a compound enzyme for an enzymolysis reaction, detecting whether the rhoifolin is completely reacted or not, and heating to extinguish the compound enzyme after the enzymolysis reaction is completed to obtain a reaction solution, wherein the compound enzyme comprises rhamnosidase and glucosidase; S2, separation: filtering the reaction solution to obtain apigenin coarse crystals, mixing the apigenin coarse crystals with an alcohol solution, and stirring until the apigenin coarse crystals are completely dissolved to obtain a complex solution; and S3, purification: cooling and crystallizing the complex solution, and filtering to obtain the apigenin. According to the method for preparing the apigenin by utilizing the biological enzymolysis, the rhoifolin is hydrolyzed into the apigenin by adopting biological enzyme catalysis, so that the method is easy and convenient to operate, green, pollution-free and suitable for large-scale production.

The invention belongs to the field of new materials. The invention relates to a novel uvioresistant fiber and a preparation method thereof. The method comprises the steps of with apigenin, a primary amine derivative, paraformaldehyde, a 2,4-dihydroxy benzophenone derivative, anhydride and cellulose serve as raw materials, carrying out a series reaction including Mannich reaction, esterification and the like to obtain a novel uvioresistant fiber material. The novel uvioresistant fiber material not only effectively solves the problem of insufficient uvioresistant capability or poor uvioresistantlasting effect of the existing cotton fiber, but also has efficient antibacterial property; the mechanical property is remarkably improved, and foreseeability shows that the novel uvioresistant fibermaterial can meet wide market space, and is particularly suitable for outdoor fields such as clothing fabrics and the like.

The invention belongs to the technical field of thermosetting resin. The invention relates to benzoxazine resin based on apigenin biology base and a preparation method thereof, especially to benzoxazine containing a phenolic hydroxyl group and carbon-carbon double bonds at the same time and preparation method thereof. The preparation method comprises the steps of: mixing apigenin, an amine compound and paraformaldehyde, adding the obtained mixture into a low-polarity solvent for reaction for 6-18 h at a temperature of 80-120 DEG C, washing the mixture with water after the reaction, performingrotary evaporation to remove an organic solvent, and drying the mixture to obtain the product. The benzoxazine resin based on apigenin biology base and the preparation method thereof have the advantages that apigenin is used as a phenol source of the benzoxazine resin, and the synthesized benzoxazine resin contains phenolic hydroxyl, so that the curing temperature can be reduced; the carbon-carbondouble bonds provided by apigenin can be used for further improving the crosslinking density of the benzoxazine resin through crosslinking reaction; and moreover, the synthesis steps are simple, theyield is high, the cured benzoxazine resin has very excellent thermal and mechanical properties, the synthesis process is easy to implement, and the benzoxazine resin is suitable for large-scale production.

The invention discloses a buddleja officinalis extract, a preparation method thereof, and an application of the buddleja officinalis extract to preparation of a medicine for treating or preventing retinal degenerative changes. The buddleja officinalis extract is obtained by extracting buddleja officinalis with an aqueous solution, and the total weight of five compounds including apigenin-7,4'-di-O-beta-D-glucosiduronic acid, echinacoside, verbascoside, isoverbascoside and apigenin-7-O-rutinoside accounts for 10% or above of the total weight of the extract. Experiments prove that the extract has good inhibitory activity on VEGFR-1 and VEGFR-2, can significantly inhibit occurrence of retinal photoreceptor cell degenerative changes, and can be used for treating or preventing retinal degenerative changes.

The invention provides a composition capable of adjusting blood sugar, blood fat and blood pressure. The composition comprises the following active components: ginsenoside Rh4 and apigenin in a weightratio of 0.5: 1 to 2: 1. The composition can simultaneously lower blood sugar, blood fat and blood pressure, has substantial synergistic effect, and can be used for preventing and treating diabetes,hyperlipidemia, hypertension, and complications caused by diabetes, hyperlipidemia and hypertension.

The invention provides a diluent preparation capable of prolonging the normal-temperature survival time of boar semen. The diluent preparation is prepared by adding an antioxidant into a basic diluent and mixing the antioxidant with the basic diluent, wherein the mass-volume ratio g/L of the antioxidant to the basic diluent is (0.03-0.15): 1. Each liter of the basic diluent contains the following raw materials by mass: 15.0-50.0 g of glucose, 0.5-5.0 g of ethylenediamine tetraacetic acid, 0.2-0.5 g of tea polyphenol, 5.0-15.0 g of sodium citrate dihydrate, 0.05-5.0 g of sodium bicarbonate and 0.02-3.0 g of potassium chloride. The antioxidant is prepared from apigenin, phenethyl caffeate, cumaric acid and plumbago zeylanica extract in a mass ratio of (0.3-0.8): (2-5): (1.3-2): 1. The basic raw materials and the dosage proportion of the diluent preparation are optimized, the survival time of the boar semen is prolonged under the normal temperature condition, sperm indexes related to the fertilization capacity are maintained, and the diluent preparation has certain guiding significance for obtaining high-quality, high-yield and low-cost boar semen stored at the normal temperature.