40 results about "Apigetrin" patented technology

The application discloses application of total flavones of chrysanthemum morifolium ramat and metabolites thereof in preparation of uric acid-lowering or liver and kidney protecting drugs or health care products. The total flavones of chrysanthemum morifolium ramat contain various flavone compounds. In a hyperuricemia model mouse, the total flavones of chrysanthemum morifolium ramat can significantly reduce blood uric acid level, and has a significant protective effect on liver and kidney, and the effect is achieved by inhibiting the activity and expression of xanthine oxidase and adenosine deaminase in the liver, and regulating the expression of uric acid related transporters in the kidney. Meanwhile, metabolites of the total flavones of chrysanthemum morifolium ramat, luteolin-7-O-glucuronide and apigenin-7-O-glucuronide, can also play a pharmacological role in the mouse body. In vitro experiments and mouse primary hepatocytes further prove that luteolin-7-O-glucuronide and apigenin-7-O-glucuronide have an inhibiting effect on the activity and expression level of xanthine oxidase. The application widens the application range of traditional Chinese medicinal materials, and provides a new direction for development of efficient and low-toxic uric acid-lowering and anti-gout drugs or health care products.

This invention belongs to the field of biomedical technology, and particularly relates to a pharmaceutical composition for treating cervical cancer and its preparation method. The pharmaceutical composition for treating cervical cancer of this invention comprises the following components: a apigenin derivative and acetylvalerin in a mass ratio of 1:(0.3~0.5). This invention obtains a apigenin derivative by introducing specific functional groups onto apigenin, which exhibits high pharmacological activity against cervical cancer; synergistically acting with acetylvalerin, it effectively kills cervical cancer cells while reducing damage to normal cells, thus providing a safer and more effective treatment option for cervical cancer patients.

The invention relates to the technical field of plant extraction, in particular to a rose extract. The rose extract comprises at least one of monomer ellagic acid, urolithin M5 and apigenin. The preparation method comprises the following steps: uniformly mixing rose powder with the ternary synergistic solvent, carrying out ultrasonic treatment for 10-60 minutes under the ultrasonic power of 150-250 W, and then stirring for 1-5 hours at the temperature of 20-50 DEG C; centrifuging and collecting supernate to obtain the rose extract. The rose flower extract provided by the invention has relatively strong inhibition capability on human pancreatic lipase.

The application discloses a bacterial QS inhibitor and application thereof, and the active ingredient of the bacterial QS inhibitor is apigenin, and the use concentration is a sub-inhibitory concentration of target bacteria. The application first discovers and systematically proves that apigenin has a wide and significant inhibitory effect on a variety of bacterial QS systems and a spoilage phenotype regulated by the QS systems. Experiments show that under a sub-inhibitory concentration, the inhibition rate of apigenin on biofilm formation of pectobacterium is up to 96.33%, the inhibition rate of apigenin on synthesis of a signal molecule is up to 88.42%, and the inhibition rate of apigenin on a key virulence enzyme is more than 70%; the fresh-keeping effect of apigenin on food is significant, and the application of apigenin in the field of food fresh-keeping is expanded; and apigenin is a natural dietary ingredient and has high safety.

This invention discloses a apigenin-betaine compound and its preparation method. The compound uses apigenin (Api) as its core, with -O-(CH2) at the 7', 4', and / or 5-position hydroxyl groups. n -N + (CH3)2-CH2COO ‑ Fragment substitution (n=2~12, x=1~3) forms an internal salt-type zwitterionic structure. In preparation, apigenin is first reacted with bromool Br(CH2). n A mitsunobu reaction was performed on OH under triphenylphosphine / dialkyl azodicarbonate conditions to obtain mono / polysubstituted bromoalkoxylated apigenin; subsequently, nucleophilic substitution with N,N-dimethylglycine under alkaline conditions yielded the target product. This structure, possessing both a tunable alkyl chain and a betaine cation / anion pair, significantly enhances the solubility and hydration capacity of apigenin in aqueous / alcoholic media, strengthens its affinity and stability for biomembranes, thereby improving in vivo absorption and distribution and potentially maintaining its antioxidant and anti-inflammatory activities. The method is mild, with a wide solvent and temperature range, suitable for scale-up and formulation applications.

The invention discloses a method for performing carbon glycoside deglycosylation by using a biological method. The invention provides application of the protein combination: application in splitting C-C glucosidic bonds; application in preparation of aglycones; the invention also relates to an application in converting glucoside into aglycone. The invention also relates to an application in converting isoorientin and isovitexin into luteolin and apigenin. The invention also relates to an application in converting isoorientin into luteolin. The invention also relates to an application in converting isovitexin into apigenin. Application in splitting C-C glucosidic bonds; the invention also relates to an application in converting mangiferin into mangiferin aglycone. The protein combination comprises an LE1 protein, an LE2 protein and an LE3 protein. The method has great application and popularization values in the production field of aglycones or aglycone derivatives and downstream products thereof.

The application discloses a kind of high-efficiency interception polyvalent anion and cation nanofiltration membrane and its preparation method and application.The method includes the following preparation process:1) preparation concentration is 0.1-2wt% of apigenin class of substance aqueous solution, and concentration is 0.1-0.5wt% of multi-acid chloride organic phase solution;2) make polymer support membrane in aqueous solution 1-5min, remove the surface excess solution, then immerse into oil phase solution, react 1-15min;3) after reaction, remove the surface excess solution, solidify 5-20min under 60-80 DEG C, obtain the nanofiltration membrane.The application is with apigenin, apigenin-7-o-glucuronide and / or isofraxidin as aqueous monomer, and multi-acid chloride is prepared by interfacial polymerization reaction to obtain negative NF membrane, not only maintain the high interception rate to polyvalent anion, and also have very high interception rate to polyvalent cation, can efficiently permeate monovalent ion, and water flux is larger.

The invention provides a construction method and application of a specific chromatogram of Pinger honey cream. The construction method comprises the following steps: a) preparation of a reference substance solution: respectively adding methanol to dissolve caffeic acid, linarin, luteolin and apigenin as reference substances to obtain the reference substance solution; b) preparation of a test solution: mixing the Pinger honey paste with a methanol solution, performing heating reflux treatment or ultrasonic treatment, cooling, and filtering to obtain the test solution; and c) determining to obtain a high performance liquid chromatogram of the test solution and a high performance liquid chromatogram of the reference solution, and selecting a common peak from the high performance liquid chromatogram of the test solution by taking the high performance liquid chromatogram of the reference solution as a contrast to obtain the characteristic chromatogram of the Pinger honey cream. By adopting the method, the specific chromatogram of the Pinger honey cream can be obtained to characterize the quality of the Pinger honey cream, so that the stability of the medicinal materials is better evaluated, and a quality evaluation system of the Pinger honey cream is perfected.

The invention belongs to the technical field of natural medicine extracts, and relates to a polygonatum sibiricum flower bud extract as well as a preparation method, a microcapsule preparation and application thereof. The polygonatum sibiricum flower bud extract contains one or more of rutin, apigenin-6-arabinose-8-glucoside, and apigenin-6, 8-diglucoside, and is characterized in that the polygonatum sibiricum flower bud extract contains one or more of rutin, apigenin-6-arabinose-8-glucoside and apigenin-6, 8-diglucoside. By utilizing the preparation method of the polygonatum sibiricum flower bud extract and the preparation method of the microcapsule preparation, the polygonatum sibiricum flower bud extract and the microcapsule preparation of the polygonatum sibiricum flower bud extract can be better prepared respectively; the prepared polygonatum sibiricum flower bud extract and the microcapsule preparation of the polygonatum sibiricum flower bud extract can better inhibit the activity of alpha-glucosidase in vivo and in vitro, and can be used for preventing or treating diabetes mellitus with better curative effect and smaller side effect.

The invention belongs to the technical field of active component and target screening, and relates to a purple sweet potato leaf uric acid reducing active component and a potential target and a screening method thereof, the active component comprises rhododendron yellow, 3, 4, 2 ', 4', 6 '-pentahydroxychalcone, apigenin, quercetin glycoside, 6-hydroxycoumarin, 6, 6-dihydroxycoumarin, 3, 4, 2', 4 ', 6'-pentahydroxychalcone, 3, 4, 2 ', 4', 6 '-pentahydroxychalcone, 3, 4, 2', 4 '-pentahydroxychalcone, the component A is prepared from 2, 7-dihydroxy coumarin, daphnetin, trigonelline, serotonin, salidroside, sesamin and nicotinamide; the potential target spots comprise INS, PPAR-gamma, IL1-beta, PPAR-alpha, XDH and ABCG2; the method comprises the following steps: preparing purple sweet potato leaves and a sweet potato leaf water extract, determining the uric acid generation inhibition activity of the purple sweet potato leaves through an in-vitro experiment, and carrying out differential metabonomics combined network pharmacology analysis to excavate functional substances for reducing uric acid in the purple sweet potato leaves, thereby providing a material basis for high-valued utilization of the purple sweet potato leaves and development of functional foods for reducing uric acid.

This invention discloses a method for preparing an oral nanoparticle formulation of apigenin, belonging to the field of traditional Chinese medicine preparation technology. The method uses a mixture of soybean protein and xylan as the aqueous phase and an apigenin ethanol solution as the organic phase. Nanoparticles are prepared by antisolvent precipitation, followed by adsorption of tannic acid to obtain the final product. The nanoparticles prepared by this invention have a particle size of less than 600 nm and a high encapsulation efficiency. Utilizing the synergistic effect of soybean protein, xylan, and tannic acid, apigenin is protected from gastric acid degradation, achieving targeted intestinal delivery and significantly improving its oral bioavailability. This method can be used to prepare drugs for treating intestinal diseases such as inflammatory bowel disease, irritable bowel syndrome, or colorectal cancer.

The application relates to preparation and application of modified whey protein, especially showing good embedding effect in embedding apigenin. The steps of the preparation method are as follows: (1) 49 mL of deionized water is used to dissolve 0.5 g of whey protein in a beaker, 1 mL of 5M hydrogen peroxide solution which has been prepared is added into the beaker after the protein is fully dissolved, 0.25 g of ascorbic acid is weighed and added, uniform mixing is carried out, and then the mixture is placed at room temperature for 2 h. (2) 0.35 mM of EGCG and 1 mM of CaCl2 are added into the protein solution after 2 h, the polyphenol and CaCl2 are stirred to be dissolved, and then the solution is placed at room temperature for reaction for 24 h. (3) after the reaction is completed, the solution after the reaction is dialyzed by using a 3500D dialysis bag to remove the polyphenol which is not combined on the protein, and the solution is dialyzed at 4 DEG C for 48 h, and the dialysis liquid is replaced every 6 h. 2+ The grafting rate of WP-EGCG is improved, the functional properties of the whey protein are enhanced, and the embedding rate of apigenin is preferably improved.

This invention discloses a diglyceride-based edible oil for assisting in lowering uric acid and its preparation method, relating to the field of edible oil technology. This diglyceride-based edible oil for assisting in lowering uric acid comprises the following ingredients in parts by weight: 42-50 parts modified high-oleic sunflower seed oil, 8-12 parts food-grade glycerol, 1.5-2.5 parts immobilized Rhizopus oryzae lipase, 0.3-0.8 parts modified apigenin, 0.2-0.4 parts vitamin E, 0.3-0.5 parts monoglyceride citrate, and 1.0-1.5 parts hydroxytyrosol. This composite edible oil combines the health benefits of diglycerides with the function of assisting in lowering uric acid. It exhibits good compatibility and stability of active ingredients, high bioavailability, excellent physicochemical properties, suitability for various cooking methods, and easy storage.

The invention relates to the technical field of biological medicine, in particular to a cyclodextrin supramolecular drug delivery system for treating chloasma. The system is composed of a cyclodextrin organic framework carrier (MOF / TOF) and a drug loaded in a pore channel of the cyclodextrin organic framework carrier; the medicine comprises bergenin, apigenin, quercetin and the like, the loading capacity is 0.1%-50%, and the average particle size is 50-500 nm. The supramolecular drug delivery system realizes intelligent drug release by responding to chloasma lesion microenvironment (including rising of active oxygen, weak acidity and high tyrosinase activity), solves the problems of poor water solubility and the like of natural drugs, and synergistically resists inflammation and oxidation and inhibits melanin synthesis. The supramolecular drug delivery system is prepared by adopting an incubation method / rotary evaporation method, and the process is mild; the pharmaceutical composition can be prepared into dosage forms such as hydro-optical needles, gel and the like, is used for treating chloasma pigment disorder dermatosis, is particularly suitable for diseases in high-altitude areas, and is high in targeting property and low in side effect.

The present application relates to the field of pharmaceutical chemistry, and particularly relates to application of apigenin and derivatives thereof or pharmaceutically acceptable salts thereof in preparation of drugs for treating or preventing kidney diseases, for example, chronic renal failure diseases. The research results of the present application show that apigenin can obviously improve symptoms of kidney diseases, especially chronic renal failure diseases.

The invention relates to the technical field of medicines, and particularly discloses a pharmaceutical preparation for treating arthritis and a preparation method thereof, and the pharmaceutical preparation is prepared from the following raw materials: 1-5 parts by weight of triptolide, 20-50 parts by weight of apigenin malate, 5-15 parts by weight of panax notoginseng saponins, 5-15 parts by weight of glycyrrhizic acid and 0.1-10 parts by weight of medicinal polypeptide. According to the application, triptolide is taken as a core attack unit, and active attenuation is realized by introducing glycyrrhizic acid; the apigenin modified by malic acid esterification is added, so that the multi-target anti-inflammatory activity of the apigenin is reserved, and meanwhile, the bioavailability problem of the apigenin is thoroughly solved; the panax notoginseng saponins are supplemented to improve microcirculation; and the medicinal polypeptide is introduced, so that accurate intervention is performed on inflammation and bone destruction pathways.

Disclosed are compositions and / or methods of use of the compositions for patients with neuronal diseases such as AD, Parkinson's, Huntington's, multiple sclerosis, and ALS. In certain embodiments flavonoids alone, or in a pharmaceutical preparation, are administered through the nasal olfactory route. In certain embodiments the flavonoid is apigenin and the neural disease is Alzheimer's. Targeted dosages may affect mitochondrial function in brain neural cells thus ameliorating a neural disease. In some embodiments a porosome complex is administered for reconstitution into a neural cell. In certain embodiments, one or more macrocyclic or linear peptides are administered.

Disclosed in the present invention is a composition for preventing, alleviating or treating periodontitis, the composition containing, as an active ingredient, a potentilla chinensis extract, apigenin-7-O-β-glucuronide, or a sitologically acceptable salt thereof. The present invention inhibits the mRNA expression of COX-2, IL-6, and MMP-3 and reduces the CEJ-ABC distance, thus suppressing inflammation, protecting the periodontium, and mitigating bone loss. Therefore, the present invention has the effects of preventing, alleviating or treating periodontitis.

The invention discloses a low-yellowing regenerated PET (Polyethylene Terephthalate) material as well as a preparation method and application thereof, and relates to the technical field of high polymer materials. When the low-yellowing regenerated PET material is prepared, waste polyester is subjected to alcoholysis with ethylene glycol and then subjected to ester exchange with methanol, and regenerated dimethyl terephthalate is prepared; carrying out polymerization on the regenerated dimethyl terephthalate, the functionalized dimethyl ester, 2-vinyl-propane-1, 3-diol and ethylene glycol to prepare regenerated PET (Polyethylene Terephthalate); the regenerated PET and furfuryl mercaptan are subjected to a reaction, and modified regenerated PET is prepared; the preparation method comprises the following steps: sequentially reacting apigenin with isophorone diisocyanate and N-(2-aminoethyl) maleimide to obtain modified apigenin; uniformly mixing the modified regenerated PET and the modified apigenin, and performing extrusion molding to obtain the low-yellowing regenerated PET material. The low-yellowing regenerated PET material prepared by the invention has the advantages of yellowing resistance, ultraviolet resistance and self-healing.

The invention discloses diglyceride composite edible oil for assisting in lowering blood pressure and a preparation method thereof, and relates to the technical field of functional edible oil. The diglyceride composite edible oil for assisting in lowering the blood pressure is prepared from the following raw materials in parts by weight: 60 to 70 parts of diglyceride oil, 7 to 11 parts of modified soybean protein isolate, 4 to 8 parts of modified apigenin, 1 to 4 parts of soybean lecithin, 0.5 to 2 parts of sucrose fatty acid ester, 0.05 to 0.3 part of sweet orange essential oil, 0.08 to 0.2 part of tea polyphenol and 0.05 to 0.15 part of vitamin E. The diglyceride composite edible oil assisting in lowering blood pressure has remarkable advantages, high-purity diglyceride oil is selected to be matched with various effective components, and the stability and absorbability of functional components are improved through a precise modification process. Advanced technologies such as multi-stage emulsification and high-pressure homogenization are adopted in the preparation process, so that the components are uniformly dispersed, the particle size reaches the standard, and impurities are few. The product has the efficacy of assisting in lowering blood pressure, has good edible safety, and is fresh in flavor and high in practicability.

The present application provides a kind of drug composition and its application for targeting neuroinflammation, the composition is composed of active ingredient and natural nanometer delivery carrier for encapsulating active ingredient, the active ingredient includes fisetin, pyrrole quinoline quinone, resveratrol, bauhinia A, apigenin, saffron, quercetin and hair monkey element;The natural nanometer delivery carrier is the plant source extracellular vesicle of surface modification brain targeting molecule.The composition of the present application realizes the intervention to vascular cognitive impairment by multi-component scientific compatibility, combined with two-stage brain targeting delivery design, solves the pain points of low blood-brain barrier penetration rate and poor bioavailability of natural active ingredient, has the effect of strong anti-inflammatory and significantly improving cognitive function, excellent safety, can be used for preparing the medicine for preventing or adjuvant therapy neuroinflammation related vascular cognitive impairment, has very high clinical conversion value.

The invention relates to medical cold compress gel for improving gout and a preparation method of the medical cold compress gel. The medical cold compress gel comprises the following components in percentage by weight: (1) a cooling matrix material: 0.5-2% of carbomer, 5-15% of glycerol and 3-8% of propylene glycol; (2) natural anti-inflammatory components: 1-3% of a radix scutellariae extract and 0.5-1.5% of asiaticoside; (3) a uric acid inhibitor: 0.2%-1% of apigenin nano-liposome and 0.1%-0.5% of chicoric acid; (4) a transdermal penetration enhancer: 1-3% of azone and 0.5-2% of menthol; and the balance of deionized water. The invention has the following beneficial effects: 1, physical-chemical synergistic effect: red and swollen can be relieved by cold compress, and inflammatory factors such as IL-1beta, TNF-alpha and the like can be inhibited by the active components; 2, targeted inhibition of uric acid: apigenin nano-liposome enhances transdermal absorption and inhibits the activity of xanthine oxidase; 3, the safety is high: the systematic side effects of the traditional medicine are reduced by the natural components;

The application discloses a plant source composition which is composed of eucalyptus leaf essential oil, eugenol essential oil and apigenin, and discloses a preparation method of the composition and application of the composition in resisting chicken coccidiosis, and belongs to the field of disease prevention and treatment of poultry and livestock and feed additive. The composition can resist Eimeria tenella, and has a promoting effect on the growth performance of chickens, is a new type of feed additive with small toxic and side effects and good insecticidal effect, has low production cost and good stability, and has great application prospect in the aspect of coccidiosis resistance of poultry.

The invention relates to an application of apigenin in preparation of drugs for enhancing NK cell functions, and the NK cell functions comprise up-regulation of expression of an NK cell activation receptor NKp46 and in-vivo and in-vitro significant improvement of the ability to kill breast cancer. Apigenin can significantly increase expression of an NK cell activated receptor NKp46 and significantly promote NK cells to kill breast cancer cells in vitro; the NK cells pretreated by the apigenin significantly inhibit tumor growth of breast cancer mice; the infiltration degree and killing ability of the NK cells pretreated by the apigenin in breast cancer are remarkably improved; apigenin pretreatment can enhance PGC1 alpha / TFAM signal transduction in NK cells and regulate and control mitochondrial metabolism. The invention provides an effective traditional Chinese medicine scheme for enhancing the treatment effect of the NK cells on the breast cancer.

The invention discloses apigenin-chitosan composite coating liquid and a strawberry preservation method, and belongs to the technical field of preservation of picked fruits and vegetables. The apigenin-chitosan composite coating liquid comprises the following components: chitosan or a derivative thereof, apigenin or an apigenin-containing extract, and an acidic aqueous solution, wherein the mass volume concentration of the apigenin or the apigenin-containing extract in the composite coating liquid is 0.1%-0.3% (w / v), and the mass volume concentration of the chitosan in the composite coating liquid is 1.0%-1.5% (w / v). According to the invention, apigenin with a specific concentration and chitosan are physically blended to prepare the film coating liquid. When the apigenin-chitosan composite preservative is used for strawberry coating preservation, the specific proportion can trigger the synergistic effect between the apigenin and the chitosan, so that the weight loss ratio and the rotting index of strawberries in the storage period are remarkably reduced, and the vitamin C content, the total acid content and the fruit hardness are effectively kept. The apigenin-chitosan composite film coating liquid and the strawberry preservation method have the advantages that the raw materials are natural, the process is simple, and the apigenin-chitosan composite film coating liquid is provided.

Disclosed are compositions and / or methods of use of the compositions for patients with neuronal diseases such as AD, Parkinson's, Huntington's, multiple sclerosis, and ALS. In certain embodiments flavonoids alone, or in a pharmaceutical preparation, are administered through the nasal olfactory route. In certain embodiments the flavonoid is apigenin and the neural disease is Alzheimer's. Targeted dosages may affect mitochondrial function in brain neural cells thus ameliorating a neural disease. In some embodiments a porosome complex is administered for reconstitution into a neural cell. In certain embodiments, one or more macrocyclic or linear peptides are administered.

The invention provides a method for detecting the content of five components in melastoma dodecandrum and application of the method. Specifically, the invention provides a method for detecting the contents of chlorogenic acid, codonopsis pilosula glycoside I, luteoloside, apigenin 7-O-beta-D-glucoside and lobetyolin in the centella asiatica. The detection method disclosed by the invention is accurate, high in sensitivity, good in repeatability and reliable in result, provides a basis for quality control and evaluation of the round-clock flowers, and has a good application prospect in formulating a round-clock flower quality standard, reasonably developing and utilizing resources and the like.

This invention belongs to the field of fluorescent and antibacterial carbon nanomaterials technology, specifically relating to a method for preparing and applying fluorescent carbon quantum dots using apigenin as the carbon source. Apigenin powder is dispersed in a 0.01-1M KOH solution via ultrasonic dispersion, then transferred to a high-pressure reactor for hydrothermal reaction. After heating, the mixture is naturally cooled to room temperature. The reaction product is then filtered and freeze-dried to obtain apigenin carbon quantum dot powder. The obtained apigenin carbon quantum dots exhibit antibacterial properties against Staphylococcus aureus and Escherichia coli under ultraviolet light irradiation. This invention offers a low-cost and simple method, producing carbon quantum dots with strong luminescence, good water solubility, stable luminescence at alkaline pH, and highly efficient photodynamic antibacterial properties, showing broad application prospects in the biological field.

The present invention relates to a medium composition for promoting muscle cell differentiation, the medium composition comprising apigenin as an active ingredient. It was confirmed that apigenin promotes the differentiation of muscle cells through regulation of the expression of muscle-specific markers and the promotion of creatine kinase activity, and thus the medium composition can be effectively used as a composition for promoting muscle cell differentiation or producing cultured meat.