The invention discloses a two-dimensional nano material and light synergistically enhanced
intracellular delivery method for cells. According to the method, efficient
intracellular delivery of cells can be synergistically realized under the mediation of exogenous pulse
laser by using a two-dimensional material. A two-dimensional material is synthesized through an HF
etching method, after the two-dimensional material and cells are co-incubated for a period of time, model molecules (fluorescent macromolecules,
protein,
nucleic acid and the like) are added, after exogenous pulse
laser is used for treatment, the two-dimensional material absorbs heat to enable surrounding water to be vaporized to generate bubbles, the bubbles are continuously expanded through
continuous irradiation of the exogenous pulse
laser, and therefore the two-dimensional material is obtained. When a certain degree is reached,
mechanical force generated by rupture enables
cell membranes and
cell wall opening biological functional macromolecules (
protein,
nucleic acid and the like) to enter cells through
free diffusion, and exogenous functional macromolecules are integrated with
genetic materials in the cells, so that long-term stable expression is realized. By adjusting the
laser energy density and various parameters (material concentration, material size,
laser energy density and the like), high
delivery efficiency is guaranteed, and cells also have
high activity. The characterization of the material by various test means shows that the characterization is successful, and meanwhile, efficient
intracellular delivery of cells is also realized.