60 results about "Cyanidin" patented technology

According to the preparation method disclosed by the invention, the whole fruit juice of the lonicera caerulea (which retains fruit residues) is fermented by virtue of the plant lactobacillus KLDS1.0391, KLDS1.0320 or KLDS1.0368, so that the xanthine oxidase (XO) inhibition activity is remarkably improved. After fermentation, the XO inhibition rate is increased to 48-50.87% (the content of unfermented juice is 31.21%), the total phenol content is increased by 15-24.3%, and the contents of main XO inhibitors in the lonicera caerulea: cyanidin-3-O-glucoside (IC50 = 115.28 [mu] M), cyanidin-3, 5-diglucoside (IC50 = 116.34 [mu] M), peonidin-3-glucoside (IC50 = 105.34 [mu] M), quercetin (IC50 = 88.75 [mu] M), catechin (IC50 = 110 [mu] M) and caffeoylquinic acid (IC50 = 250.35 [mu] M) are remarkably increased. Wherein the quercetin content is increased by 73.8% at most (KLDS1.0320 strain fermentation is performed for 24 hours), and the XO inhibition rate is increased from 31.21% to 50.87%. Technical support is provided for development of a natural XO inhibitor and a functional fermented beverage, so that a new thought is provided for functional fermented juice with lonicera caerulea as a key component.

The invention discloses a planting method for increasing the content of anthocyanin in purple rice grains, which comprises the following specific steps: sowing purple rice seeds from May 20th to May 30th every year, and setting the nitrogen application amount to be 24kg N / mu and the planting density to be 1.58-2.22 million holes / mu at the same time. According to the method, the sowing time, the nitrogen application amount and the planting density are precisely and cooperatively regulated and controlled, resource distribution in the key period of purple rice anthocyanin synthesis is directionally optimized, the content of paeoniflorin and cyanidin in grains is remarkably increased, meanwhile, yield stability and efficient resource utilization are both considered, and through cultivation technology innovation which is low in cost and easy to popularize, the yield of purple rice is increased. The multi-target of directional enhancement of functional components of purple rice, stable yield and high resource efficiency is achieved, a technical normal form is provided for green transformation of functional agriculture, remarkable economic, ecological and social benefits are achieved, and the important requirement for green and high-efficiency development of functional agriculture is met.

The invention relates to the technical field of food packaging, in particular to a dual-antioxidant packaging film as well as a preparation method and application thereof. The dual antioxidant packaging film disclosed by the invention is prepared from the following components in parts by mass: 3.2 to 4 parts of starch, 0.8 to 1 part of glycerol, 0.8 to 1 part of polysaccharide, 0.02 to 0.16 part of berberine / anthocyanin self-assembled nanoparticles and 93.98 to 95.2 parts of water. The packaging film prepared by the invention is uniform in structure, has good mechanical properties and moisture and oxygen barrier properties, shows an intelligent slow release characteristic in a pH value change or oxidation environment, can realize controllable release of the tea tree essential oil, and significantly improves the antioxidant and antibacterial activity of the film. The material source is natural, renewable and biodegradable, and the environment-friendly fresh-keeping packaging material is suitable for fresh-keeping packaging of foods such as fruits, vegetables and meat products, and has good environment friendliness and industrial application prospects.

The invention provides a preparation method of a water-responsive shrinkage collagen membrane. The preparation method comprises the following steps: providing a cyanidin-coated gallium-indium liquid metal and a collagen solution; mixing the cyanidin-coated gallium-indium liquid metal with a collagen solution, and carrying out vacuum drying to obtain slices; and salting out the slices by using a sodium citrate solution, and stretching to obtain the water-responsive shrinkage collagen membrane. The collagen membrane prepared by the method disclosed by the invention has excellent water response shrinkage performance, can be quickly shrunk under the stimulation of water, and also has excellent mechanical properties, electrical conductivity and oxidation resistance.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The present invention addresses the problem of providing an inkjet ink which has excellent removability, that is, from which the colorant can be easily removed.　Specifically, the inkjet ink contains a colorant, wherein the colorant is one or more substances selected from the group consisting of iron oxide compounds, phycocyanin, caramel, copper-chlorophyllin compounds, capsaicin, carminic acid, betanin, cyanidin glycosides, carmine, β-carotene, lutein, norbixin, and curcumin.

The invention discloses a lactobacillus paragasseri strain capable of converting anthocyanin to generate various active metabolites, inhibiting NF-kappa B and cGAS-STING signals and enhancing inflammatory response relieving, and belongs to the technical field of microorganisms and the technical field of medicines. The lactobacillus paragasseri CCFM1499 provided by the invention can be used for converting anthocyanin into various active substances such as homovanillic acid, cyanidin, 3 ', 4'-dihydroxyphenylacetic acid and the like; fermentation supernate obtained by fermenting anthocyanin through the lactobacillus paragasseri CCFM1499 has the following effects: (1) the capability of reducing inflammatory factors of cells is achieved; (2) the cell senescence phenotypic capability is relieved; (3) the cGAS-STING signal channel capability is inhibited; and (4) inhibiting the NF-kappa B signal path capability. The lactobacillus paragasseri CCFM1499 fermented anthocyanin provided by the invention can be used for preparing a product for relieving inflammatory response, and has a huge application prospect.

The invention relates to a method for extracting roselle anthocyanin through enzymolysis-ultrasonic assistance, a product and application of the product. The method comprises the following steps: degreasing roselle powder, and performing enzymolysis for 24 hours by using cellulase and pectinase (20U / g respectively) under the conditions that the pH is 5.0 and the temperature is 40 DEG C; an acetone-hydrochloric acid mixed solution (the volume fraction of hydrochloric acid is 36%) with the liquid-material ratio being 10: 1 serves as an extracting agent, and ultrasonic extraction is conducted for 34 minutes at the temperature of 58 DEG C and the power of 160 W; and finally, centrifuging, extracting with ethyl acetate, and purifying to obtain an anthocyanin product. According to the method, the extraction rate reaches 3.087 mg / g, the main component of the product is cyanidin-3-glucoside, the antioxidant and antibacterial activity is remarkable, the scavenging rates of DPPH, ABTS and hydroxyl radicals are 86.26%, 81.34% and 93.75% respectively under the concentration of 3.0 mg / mL, and the scavenging rates are superior to those of vitamin C with the same concentration; under the concentration of 11mg / mL, the compound has an obvious inhibition effect on escherichia coli and staphylococcus aureus. The process is efficient and mild, the product activity is excellent, and the method is suitable for development and application of natural antioxidants and antibacterial agents.

This invention discloses a method for preparing and applying a chitosan-nanofibrillar-based smart indicator membrane loaded with anthocyanins and alizarin. The method includes the following steps: mixing beet powder with hydrochloric acid, washing with water until neutral, and drying; adding sodium hydroxide solution, mixing and reacting, washing with water until neutral, and drying; adding sodium chlorite solution, mixing, adjusting the pH to 3-3.5, reacting, and washing with water until neutral to obtain beet fiber; adding water, homogenizing under ultra-high pressure to obtain a beet nano-suspension; adding the beet nano-suspension to a chitosan-acetic acid solution and stirring, then adding glycerol; adding pigments, mixing, and obtaining a membrane solution; ultrasonically degassing, and drying to form a membrane. This membrane, as a food preservation indicator membrane, possesses both the characteristics and functions of food preservation and indicating food freshness; using beet nanofibers as a supplementary material improves the mechanical properties of the chitosan membrane; the addition of alizarin and anthocyanin mixed pigments enriches the color changes of the indicator membrane and improves its stability.

The anthocyanin source grape seed candy is a tabletted candy prepared from peel residues obtained after wine grape fermentation is finished through the working procedures of airing, rolling, blowing, careful selection, cleaning, drying, ultraviolet sterilization, multiple times of smashing, honey adding, stirring, tabletting, vacuum packaging and the like. Only grape seeds and honey are used as raw materials, no preservative or additive is added, and a pure natural product is not subjected to chemical treatment.

The application relates to the technical field of polypeptide preparation, and particularly discloses a preparation method of animal blood globin polypeptide powder. The preparation method of the animal blood globin polypeptide powder comprises the following steps: adding trisodium citrate into fresh animal blood, stirring for 10-30 min, adding cyanidin and polylysine, stirring uniformly, placing in a temperature-10 to-20 DEG C environment for 1-2 h, increasing to room temperature, homogenizing for 1-5 min, centrifuging, removing supernatant, and obtaining animal blood globin solution; adding the animal blood globin solution and rare earth lanthanum chitosan amine chelate salt into a glycine-sodium hydroxide buffer solution, stirring at 40-50 DEG C for 10-30 min, adding compound protease, and continuing to stir for 10-20 h to obtain an enzymatic solution; adjusting the pH value of the enzymatic solution to 5-6, increasing the temperature to 90-100 DEG C for 5-10 min, decreasing to room temperature, centrifuging for 5-10 min, and taking supernatant for low-temperature spray drying.

The invention provides a preparation method of a cyanidin-3-O-glucoside-methyl sorbate acylated derivative, and belongs to the technical field of natural product modification. The method comprises the following steps: by taking cyanidin-3-O-glucoside as a substrate and methyl sorbate as an acyl donor, reacting in a low-toxicity DMF / tert-butyl alcohol (9: 1) mixed solvent under the catalysis of lipase Novozym 435, and extracting and purifying to obtain a target product. The C3G is modified by methyl sorbate containing conjugated double bonds, so that the fat solubility of the product is remarkably improved while the natural light response characteristic is reserved. A reaction system and a purification process method are optimized, the used acyl structure is more innovative, the reaction condition is mild, the conversion rate is high, few byproducts are produced, and the method is green and environment-friendly. Methyl sorbate is grafted to cyanidin-3-O-glucoside, so that the thermal stability of cyanidin-3-O-glucoside can be enhanced, the fat solubility can be improved, the problem that a light function is damaged by traditional acylation is solved, and a new raw material is provided for light-controlled drug delivery and weather-resistant food pigments.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The invention relates to the technical field of biological medicines, in particular to application of five cyanidin derivatives in preparation of medicines for treating and / or preventing neurodegenerative diseases. The cyanidin derivative provided by the invention can inhibit the activity of acetylcholin esterase, relieve oxidative stress, reduce AD related gene expression and protect neuron integrity; due to the multi-target action mechanism, the symptoms of AD patients can be more comprehensively improved, and the disease progression is delayed.

The invention discloses preparation of a defective zirconium-based metal organic framework and application of the defective zirconium-based metal organic framework in separation and purification of cyanidin anthocyanin, and belongs to the technical field of efficient extraction, enrichment and separation of natural active molecules. The preparation method comprises the following steps: firstly, preparing a zirconium-based HP-UiO-66X material by taking zirconium tetrachloride and terephthalic acid as a metal center and an organic ligand raw material respectively under a solvothermal condition; in the synthesis process, a certain amount of benzoic acid is added as a regulator, and then the benzoic acid is etched by formic acid to form defects. The synthesized defective UiO-66X material has a hierarchical porous structure, and different groups modified by the ligand can form hydrogen bonds and other interactions on anthocyanin, so that the adsorption performance of the HP-UiO-66X material on the anthocyanin is improved.

The invention discloses health wine capable of delaying senescence as well as a preparation method and application of the health wine, and belongs to the technical field of health food. According to the health-preserving wine, mulberries and sea-buckthorn are used as monarch materials, rhizoma polygonati and radix puerariae are used as ministerial materials, lophatherum gracile, fructus lycii and Chinese yam are used as assistants and guides, and all the raw materials are medicinal and edible substances. According to the invention, through an optimized'warm soaking-cold soaking 'two-step extraction process, synergistic interaction is realized, and active ingredients such as anthocyanin, flavone, polysaccharide and puerarin are extracted to the greatest extent. The health-preserving wine disclosed by the invention not only has good antioxidant capacity, but also can be used for remarkably removing free radicals such as DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl, improving the activity of serum SOD (superoxide dismutase) and GSH-Px (glutathione The formation of advanced glycosylation end products can be effectively inhibited, and the obvious anti-aging and anti-saccharification dual effects are achieved. The health-preserving wine disclosed by the invention is simple in preparation process, mellow in taste and suitable for daily health-preserving drinking.

The present application provides a betalain-cyanidin composite pigment composition, a dyeing agent and application, the composition is combined by cyanidin freeze-dried powder and betalain freeze-dried powder according to the mass ratio of 5:1-5. The betalain-cyanidin composite dyeing agent formed by the composition has good temperature stability and pH stability, and strong coloring power, and can be widely applied to product materials containing kapok, silk, cotton, hemp and the like.

The invention discloses a construction method and application of a chassis cell line for directionally synthesizing anthocyanin, and belongs to the technical field of cell line construction. The invention provides a construction method of a chassis cell line for oriented synthesis of anthocyanin, the chassis cell line obtained by the method has all genes for oriented synthesis of anthocyanin, and particularly, the purpose of oriented synthesis of a large amount of anthocyanin can be achieved by overexpressing anthocyanin in plants to synthesize transcriptional regulatory factors. According to the invention, an overexpression hairy root strain is obtained by constructing a plant overexpression vector of a transcription factor and utilizing an agrobacterium rhizogenes mediated method based on a chassis cell hairy root genetic transformation system, namely the chassis cell line is obtained. By utilizing the chassis cell line, the effect of efficiently and directionally synthesizing cyanidin through gene overexpression can be achieved, the content of cyanidin is high, and the purity of extracted anthocyanin is remarkably improved. The chassis cell line provided by the invention has the advantages of short growth cycle and simple culture mode, and can be subjected to industrial mass production.

The invention provides a co-expression vector and a method for changing the type of biosynthetic anthocyanin glycoside, and belongs to the technical field of genetic engineering. The invention provides a co-expression vector containing a gene overexpression box and a gene knockout box, and an MYB transcription factor overexpression box and an anthocyanin rhamnoside transferase gene knockout box are jointly constructed on a basic skeleton vector. Therefore, the co-expression vector can simultaneously achieve the effects of overexpressing MYB and knocking out the anthocyanin rhamnoside transferase gene. In the embodiment of the invention, by taking antirhubarb as an example, genetic transformation is completed by utilizing the co-expression vector, and gene knockout and overexpression are carried out through one vector, so that the conversion of anthocyanin from rhamnoside to glucoside is realized, for example, the conversion of cyanidin rhamnoside to cyanidin glucoside is realized, and the conversion of anthocyanin from rhamnoside to cyanidin glucoside is realized. Therefore, the content of glucoside in the anthocyanin glycoside is obviously improved.

The present invention discloses the use of cyanidin in the preparation of a drug for improving reproductive and / or growth and development ability. The compound has a significant effect on promoting the reproductive ability, motility and growth and development of Caenorhabditis elegans. By measuring the egg production, body bending frequency, head swing frequency, pharyngeal pumping frequency and body length of Caenorhabditis elegans after administration and in the control group, in the reproductive ability test, cyanidin increased the egg production of Caenorhabditis elegans and improved the reproductive ability. In the motility test, cyanidin increased the body bending, head swing frequency and pharyngeal pumping frequency of Caenorhabditis elegans, and enhanced the motility. In the body length test, cyanidin increased the body length of Caenorhabditis elegans. The above results show that cyanidin has a significant promoting effect on the growth and development and reproductive ability of Caenorhabditis elegans.

The invention provides a preparation method of a chlorinated cyanidin-3-O-alpha-arabinoside standard substance, which comprises the following steps: performing hydrochloric acid acidification ethanol extraction, macroporous resin enrichment and reversed-phase chromatography and gel chromatography purification, and combining a low-temperature C18 adsorption solidification process to realize high-purity (gt; the preparation method has the advantages of high yield (98.5%), gram-level preparation, good industrial production applicability, solving of the problems of low extraction rate, complex purification and easy decomposition of the target object in the prior art, and suitableness for large-scale production.

Non-therapeutic use of a bioactive substance for mood modulation of a subject, wherein the bioactive substance is one or more of yangonin, α-amyrin, β-amyrin, cyanidin, curcumin, catechin, quercetin,

The invention discloses an application of cyanidin-3-O-glucoside in preparation of a medicine for improving pyroptosis of liver cells caused by aflatoxin B1 (aflatoxin B1). According to the application disclosed by the invention, the C3G is found to have a very good relieving and improving effect on the pyroptosis of liver cells caused by AFB1 (Adenosine Factor B1), the effect of the C3G with a medium dose (3mg / kg) is optimal, and the C3G with the medium dose can be used for obviously inhibiting the expression level of liver pyroptosis pathway related factors and the expression of inflammatory factors such as TLR4, NLRP3 and Caspase-1. The cyanidin-3-O-glucoside is extracted from natural plants in nature, has natural activity and small toxic and side effects, has good improvement and alleviation effects on the pyroptosis of liver cells caused by AFB1, can be used as a medicine for treating the pyroptosis of liver cells caused by AFB1, and can be used as a medicine for treating the pyroptosis of liver cells caused by AFB1. And a novel detoxification scheme is provided for clinical treatment of liver injury caused by AFB1.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

The present application relates to the technical field of biological medicine, and specifically provides a visual hydrogel based on multiple response mechanisms and a preparation method and application thereof.The preparation method comprises the following steps: (1) preparing an inclusion compound by using beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin and cyanidin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / κ-carrageenan; (3) initiating dynamic crosslinking by using phenylboronic acid, and constructing a multiple network structure in cooperation with tannic acid, the inclusion compound and genipin, and then curing the hydrogel through gradient temperature control.The hydrogel has pH / temperature / ROS response characteristics, and can use the color change of cyanidin to feed back the wound microenvironment state in real time, and meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, promote the ordered arrangement of collagen, and accelerate wound healing, thereby solving the problems of unstable direct loading of cyanidin and difficulty of a single response mechanism in adapting to the complex microenvironment of a diabetic wound.

The application provides a method for synthesizing cyanidin by whole cell transformation. The method comprises the following steps: inducing culture of a recombinant strain S2; and inoculating the bacteria obtained by the induced culture into a culture medium containing a substrate 1-(4-hydroxyphenol)-ethanol to perform whole cell transformation, so as to synthesize the cyanidin. The application provides a biological transformation method for synthesizing cyanidin instead of a chemical synthesis method and a natural extraction method. The recombinant Escherichia coli is used to transform 1-(4-hydroxyphenol)-ethanol into cyanidin, and the method has the advantages of mild reaction condition, low production cost and environmental protection.

The invention discloses a genetically engineered bacterium for producing cyanidin-3-O-rutinoside as well as a construction method and application of the genetically engineered bacterium, and relates to the technical field of biological engineering. The invention provides an application of escherichia coli in production of cyanidin 3-O rutinoside. The modified recombinant escherichia coli can be used for efficient biosynthesis of cyanidin 3-O-rutinoside through whole-cell catalysis. Compared with plant extraction, the method has the advantages that the production conditions are mild, the product yield is increased, the specific synthesis of the product is realized, and an efficient and environment-friendly method with a wide development prospect is provided for the production and application of the cyanidin-3-O-rutinoside.

The invention discloses recombinant escherichia coli with high yield of cyanidin-3-O-glucoside as well as a construction method and application of the recombinant escherichia coli. The method comprises the following steps: by taking escherichia coli BL21 (DE3) engineering bacteria as a host, integrating flavanonol 4-reductase, anthocyanin synthetase and flavonoid 3-O-glucosyltransferase from different sources onto a plasmid vector pRSFDuet, and constructing to obtain a chassis cell for producing C3G; meanwhile, glutathione transferase is overexpressed, dissolution-promoting tag small ubiquitin-like modified protein is added, FaDFR is modified through enzyme engineering, FaDFR catalytic efficiency is improved through artificial protein cage space combination pathway enzyme, key precursor UDP-glucose is synthesized in an in-situ intensified mode and cofactor engineering, the cell factory for efficiently synthesizing C3G is constructed based on the combination strategy, the yield of C3G is increased, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. The titer reaches 797.20 mg / L. The method provided by the invention has the characteristics of simplicity, economy and eco-friendliness.

The present invention provides a complex for improving the tolerance and proliferation ability of probiotics, and its preparation method and application. By extracting polysaccharides from aged Xanthii Fructus and mixing them with cyanidin-3-O-glucoside (C3G) and silymarin extract to form a complex, it is used for the cultivation of probiotics, thereby enhancing the tolerance and proliferation ability of probiotics. The experimental results show that this complex can significantly improve the survival rate and proliferation ability of Lactobacillus acidophilus and Bacillus coagulans, and has application potential in the fields of functional foods, health products, etc.