32 results about "Cyanidin" patented technology

The invention relates to the technical field of food packaging, in particular to a dual-antioxidant packaging film as well as a preparation method and application thereof. The dual antioxidant packaging film disclosed by the invention is prepared from the following components in parts by mass: 3.2 to 4 parts of starch, 0.8 to 1 part of glycerol, 0.8 to 1 part of polysaccharide, 0.02 to 0.16 part of berberine / anthocyanin self-assembled nanoparticles and 93.98 to 95.2 parts of water. The packaging film prepared by the invention is uniform in structure, has good mechanical properties and moisture and oxygen barrier properties, shows an intelligent slow release characteristic in a pH value change or oxidation environment, can realize controllable release of the tea tree essential oil, and significantly improves the antioxidant and antibacterial activity of the film. The material source is natural, renewable and biodegradable, and the environment-friendly fresh-keeping packaging material is suitable for fresh-keeping packaging of foods such as fruits, vegetables and meat products, and has good environment friendliness and industrial application prospects.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The invention discloses a lactobacillus paragasseri strain capable of converting anthocyanin to generate various active metabolites, inhibiting NF-kappa B and cGAS-STING signals and enhancing inflammatory response relieving, and belongs to the technical field of microorganisms and the technical field of medicines. The lactobacillus paragasseri CCFM1499 provided by the invention can be used for converting anthocyanin into various active substances such as homovanillic acid, cyanidin, 3 ', 4'-dihydroxyphenylacetic acid and the like; fermentation supernate obtained by fermenting anthocyanin through the lactobacillus paragasseri CCFM1499 has the following effects: (1) the capability of reducing inflammatory factors of cells is achieved; (2) the cell senescence phenotypic capability is relieved; (3) the cGAS-STING signal channel capability is inhibited; and (4) inhibiting the NF-kappa B signal path capability. The lactobacillus paragasseri CCFM1499 fermented anthocyanin provided by the invention can be used for preparing a product for relieving inflammatory response, and has a huge application prospect.

The invention relates to a method for extracting roselle anthocyanin through enzymolysis-ultrasonic assistance, a product and application of the product. The method comprises the following steps: degreasing roselle powder, and performing enzymolysis for 24 hours by using cellulase and pectinase (20U / g respectively) under the conditions that the pH is 5.0 and the temperature is 40 DEG C; an acetone-hydrochloric acid mixed solution (the volume fraction of hydrochloric acid is 36%) with the liquid-material ratio being 10: 1 serves as an extracting agent, and ultrasonic extraction is conducted for 34 minutes at the temperature of 58 DEG C and the power of 160 W; and finally, centrifuging, extracting with ethyl acetate, and purifying to obtain an anthocyanin product. According to the method, the extraction rate reaches 3.087 mg / g, the main component of the product is cyanidin-3-glucoside, the antioxidant and antibacterial activity is remarkable, the scavenging rates of DPPH, ABTS and hydroxyl radicals are 86.26%, 81.34% and 93.75% respectively under the concentration of 3.0 mg / mL, and the scavenging rates are superior to those of vitamin C with the same concentration; under the concentration of 11mg / mL, the compound has an obvious inhibition effect on escherichia coli and staphylococcus aureus. The process is efficient and mild, the product activity is excellent, and the method is suitable for development and application of natural antioxidants and antibacterial agents.

This invention discloses a method for preparing and applying a chitosan-nanofibrillar-based smart indicator membrane loaded with anthocyanins and alizarin. The method includes the following steps: mixing beet powder with hydrochloric acid, washing with water until neutral, and drying; adding sodium hydroxide solution, mixing and reacting, washing with water until neutral, and drying; adding sodium chlorite solution, mixing, adjusting the pH to 3-3.5, reacting, and washing with water until neutral to obtain beet fiber; adding water, homogenizing under ultra-high pressure to obtain a beet nano-suspension; adding the beet nano-suspension to a chitosan-acetic acid solution and stirring, then adding glycerol; adding pigments, mixing, and obtaining a membrane solution; ultrasonically degassing, and drying to form a membrane. This membrane, as a food preservation indicator membrane, possesses both the characteristics and functions of food preservation and indicating food freshness; using beet nanofibers as a supplementary material improves the mechanical properties of the chitosan membrane; the addition of alizarin and anthocyanin mixed pigments enriches the color changes of the indicator membrane and improves its stability.

The anthocyanin source grape seed candy is a tabletted candy prepared from peel residues obtained after wine grape fermentation is finished through the working procedures of airing, rolling, blowing, careful selection, cleaning, drying, ultraviolet sterilization, multiple times of smashing, honey adding, stirring, tabletting, vacuum packaging and the like. Only grape seeds and honey are used as raw materials, no preservative or additive is added, and a pure natural product is not subjected to chemical treatment.

The invention provides a preparation method of a cyanidin-3-O-glucoside-methyl sorbate acylated derivative, and belongs to the technical field of natural product modification. The method comprises the following steps: by taking cyanidin-3-O-glucoside as a substrate and methyl sorbate as an acyl donor, reacting in a low-toxicity DMF / tert-butyl alcohol (9: 1) mixed solvent under the catalysis of lipase Novozym 435, and extracting and purifying to obtain a target product. The C3G is modified by methyl sorbate containing conjugated double bonds, so that the fat solubility of the product is remarkably improved while the natural light response characteristic is reserved. A reaction system and a purification process method are optimized, the used acyl structure is more innovative, the reaction condition is mild, the conversion rate is high, few byproducts are produced, and the method is green and environment-friendly. Methyl sorbate is grafted to cyanidin-3-O-glucoside, so that the thermal stability of cyanidin-3-O-glucoside can be enhanced, the fat solubility can be improved, the problem that a light function is damaged by traditional acylation is solved, and a new raw material is provided for light-controlled drug delivery and weather-resistant food pigments.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The invention discloses preparation of a defective zirconium-based metal organic framework and application of the defective zirconium-based metal organic framework in separation and purification of cyanidin anthocyanin, and belongs to the technical field of efficient extraction, enrichment and separation of natural active molecules. The preparation method comprises the following steps: firstly, preparing a zirconium-based HP-UiO-66X material by taking zirconium tetrachloride and terephthalic acid as a metal center and an organic ligand raw material respectively under a solvothermal condition; in the synthesis process, a certain amount of benzoic acid is added as a regulator, and then the benzoic acid is etched by formic acid to form defects. The synthesized defective UiO-66X material has a hierarchical porous structure, and different groups modified by the ligand can form hydrogen bonds and other interactions on anthocyanin, so that the adsorption performance of the HP-UiO-66X material on the anthocyanin is improved.

The invention discloses health wine capable of delaying senescence as well as a preparation method and application of the health wine, and belongs to the technical field of health food. According to the health-preserving wine, mulberries and sea-buckthorn are used as monarch materials, rhizoma polygonati and radix puerariae are used as ministerial materials, lophatherum gracile, fructus lycii and Chinese yam are used as assistants and guides, and all the raw materials are medicinal and edible substances. According to the invention, through an optimized'warm soaking-cold soaking 'two-step extraction process, synergistic interaction is realized, and active ingredients such as anthocyanin, flavone, polysaccharide and puerarin are extracted to the greatest extent. The health-preserving wine disclosed by the invention not only has good antioxidant capacity, but also can be used for remarkably removing free radicals such as DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl, improving the activity of serum SOD (superoxide dismutase) and GSH-Px (glutathione The formation of advanced glycosylation end products can be effectively inhibited, and the obvious anti-aging and anti-saccharification dual effects are achieved. The health-preserving wine disclosed by the invention is simple in preparation process, mellow in taste and suitable for daily health-preserving drinking.

The present application provides a betalain-cyanidin composite pigment composition, a dyeing agent and application, the composition is combined by cyanidin freeze-dried powder and betalain freeze-dried powder according to the mass ratio of 5:1-5. The betalain-cyanidin composite dyeing agent formed by the composition has good temperature stability and pH stability, and strong coloring power, and can be widely applied to product materials containing kapok, silk, cotton, hemp and the like.

The invention discloses a construction method and application of a chassis cell line for directionally synthesizing anthocyanin, and belongs to the technical field of cell line construction. The invention provides a construction method of a chassis cell line for oriented synthesis of anthocyanin, the chassis cell line obtained by the method has all genes for oriented synthesis of anthocyanin, and particularly, the purpose of oriented synthesis of a large amount of anthocyanin can be achieved by overexpressing anthocyanin in plants to synthesize transcriptional regulatory factors. According to the invention, an overexpression hairy root strain is obtained by constructing a plant overexpression vector of a transcription factor and utilizing an agrobacterium rhizogenes mediated method based on a chassis cell hairy root genetic transformation system, namely the chassis cell line is obtained. By utilizing the chassis cell line, the effect of efficiently and directionally synthesizing cyanidin through gene overexpression can be achieved, the content of cyanidin is high, and the purity of extracted anthocyanin is remarkably improved. The chassis cell line provided by the invention has the advantages of short growth cycle and simple culture mode, and can be subjected to industrial mass production.

The invention provides a co-expression vector and a method for changing the type of biosynthetic anthocyanin glycoside, and belongs to the technical field of genetic engineering. The invention provides a co-expression vector containing a gene overexpression box and a gene knockout box, and an MYB transcription factor overexpression box and an anthocyanin rhamnoside transferase gene knockout box are jointly constructed on a basic skeleton vector. Therefore, the co-expression vector can simultaneously achieve the effects of overexpressing MYB and knocking out the anthocyanin rhamnoside transferase gene. In the embodiment of the invention, by taking antirhubarb as an example, genetic transformation is completed by utilizing the co-expression vector, and gene knockout and overexpression are carried out through one vector, so that the conversion of anthocyanin from rhamnoside to glucoside is realized, for example, the conversion of cyanidin rhamnoside to cyanidin glucoside is realized, and the conversion of anthocyanin from rhamnoside to cyanidin glucoside is realized. Therefore, the content of glucoside in the anthocyanin glycoside is obviously improved.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

The application provides a method for synthesizing cyanidin by whole cell transformation. The method comprises the following steps: inducing culture of a recombinant strain S2; and inoculating the bacteria obtained by the induced culture into a culture medium containing a substrate 1-(4-hydroxyphenol)-ethanol to perform whole cell transformation, so as to synthesize the cyanidin. The application provides a biological transformation method for synthesizing cyanidin instead of a chemical synthesis method and a natural extraction method. The recombinant Escherichia coli is used to transform 1-(4-hydroxyphenol)-ethanol into cyanidin, and the method has the advantages of mild reaction condition, low production cost and environmental protection.

The invention discloses recombinant escherichia coli with high yield of cyanidin-3-O-glucoside as well as a construction method and application of the recombinant escherichia coli. The method comprises the following steps: by taking escherichia coli BL21 (DE3) engineering bacteria as a host, integrating flavanonol 4-reductase, anthocyanin synthetase and flavonoid 3-O-glucosyltransferase from different sources onto a plasmid vector pRSFDuet, and constructing to obtain a chassis cell for producing C3G; meanwhile, glutathione transferase is overexpressed, dissolution-promoting tag small ubiquitin-like modified protein is added, FaDFR is modified through enzyme engineering, FaDFR catalytic efficiency is improved through artificial protein cage space combination pathway enzyme, key precursor UDP-glucose is synthesized in an in-situ intensified mode and cofactor engineering, the cell factory for efficiently synthesizing C3G is constructed based on the combination strategy, the yield of C3G is increased, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. The titer reaches 797.20 mg / L. The method provided by the invention has the characteristics of simplicity, economy and eco-friendliness.

The present application relates to a kind of heterogenous amyloid fibril-cyanidin-3-O-glucoside (C3G) complex and its application.The preparation method includes: glutelin and soy protein are dispersed in aqueous phase according to mass ratio 3:7-5:5, and are co-assembled to form heterogenous amyloid fibril under acid heat condition;The heterogenous amyloid fibril solution is mixed with C3G solution, and is dried, to obtain heterogenous amyloid fibril-C3G complex.The complex can reduce the hydrolysis rate of rice starch under in vitro digestion condition after being compounded and gelatinized with rice starch.

This invention provides a method for preparing a black rice starch-anthocyanin complex and its application, comprising the following steps: mixing black rice starch and anthocyanins, and extruding the mixture using a twin-screw extruder to obtain the complex; wherein the amount of anthocyanins added is 0.5-5% of the weight of the black rice starch; the anthocyanins are cyanidin-3-O-glucoside or crude anthocyanins extracted from black rice. This invention regulates the starch molecular structure through cyanidin-3-O-glucoside or crude anthocyanins, reducing rapidly digestible starch, increasing resistant starch content, and lowering the predicted glycemic index, and can be widely applied to the development of low-GI foods, diabetic supplements, and functional staple foods.

The invention relates to acylated cyanidin-3-O-glucoside as well as a preparation method and application thereof, and belongs to the technical field of pigment processing. According to the method, the thermomyces lanuginosus lipase is used as a catalyst, so that the enzyme cost is remarkably reduced while efficient catalysis is ensured; the problem that in the prior art, the catalytic efficiency of medium-chain and short-chain fatty acid is low is solved, better catalytic efficiency is shown in medium-chain and short-chain fatty acid catalysis, and the substrate conversion rate can reach 97% and is remarkably superior to that of Novozym 435. The fat solubility of the acylated cyanidin-3-O-glucoside prepared by the method disclosed by the invention is obviously improved, the acylated cyanidin-3-O-glucoside has better stability under acid-base, heat, high temperature and high pressure and ultraviolet conditions, and the acylated cyanidin-3-O-glucoside prepared from C6-C16 fatty acid has higher antioxidant activity; and a scientific basis is provided for molecular design of the acylated cyanidin-3-O-glucoside in application scenes such as grease foods, emulsion cosmetics and drug delivery systems.

The invention discloses a preparation method of Kushui rose fermentation liquor and application of the Kushui rose fermentation liquor in anti-aging cosmetics. According to the preparation method, Kushui rose is taken as a substrate, lactobacillus plantarum TS337821 is taken as zymophyte, and fermentation is carried out under optimal conditions (for example, the inoculation amount is 3%, and fermentation is carried out at 37 DEG C for 45 hours). According to the method, the content of active ingredients such as chlorogenic acid and cyanidin-3-O-glucoside in the fermentation liquor can be remarkably increased. Experiments prove that the obtained fermentation liquor has a definite anti-aging effect, and can effectively improve cell viability, promote synthesis of collagen I, inhibit matrix metalloproteinase-I and enhance activity of antioxidant enzymes in cells. The fermentation liquor can be directly used for preparing anti-aging cosmetics, and the recommended volume fraction in a formula is 0.75%. The invention provides a new technical scheme and a high-quality raw material for high-value development of the Kushui rose.

The present application belongs to the technical field of plant extracts, and particularly relates to a process for efficiently extracting high-activity blackflower akebia anthocyanidins. The present application uses a new lactic acid / xylose alcohol DES as an extraction solvent and cooperates with a deep cold cell wall breaking technology to efficiently extract high-activity blackflower akebia anthocyanidins through centrifugal high-speed stirring. The extraction process significantly improves the extraction rate of active substances, shortens the extraction time, maximally retains the activity of anthocyanidins, and is green, safe, and low in cost.

This invention relates to a polymeric probe for assessing sialylation levels in vivo. It uses a dendritic polymer with alkyne-terminated branches as a carrier, and its surface is simultaneously modified with galactose, cyanidin 5 fluorescent dye, and folic acid. The detection protocol involves subcutaneously injecting the prepared polymeric probe into the tumor region of tumor-bearing mice. Folic acid-mediated targeted endocytosis allows the polymeric probe to enter the tumor cells. In vivo sialylates bind sialic acid to the galactose terminal of the polymeric probe. Sodium periodate is used to oxidize the sialic acid terminal in mouse tumor tissue sections to form an aldehyde group, which is then further coupled with an acylhydrazide fluorescein molecule. Fluorescence signals from cyanidin 5 and fluorescein are simultaneously collected using fluorescence microscopy, and the fluorescence intensity of fluorescein in the superimposed region of the two fluorescence signals is obtained using software, which can then be used to assess the sialylation level of the tumor tissue.

A method for constructing a chassis cell line used for directed synthesis of an anthocyanin and the use thereof, belonging to the technical field of cell line construction. The chassis cell line obtained by the method comprises all genes for the directed synthesis of the anthocyanin; and, in specific, by means of overexpressing a transcriptional regulation factor for anthocyanin synthesis in plants, the purpose of directed synthesis of the anthocyanin in great quantity can be achieved. By constructing a plant overexpression vector for the transcription factor, and using Agrobacterium rhizogenes for mediation, a hairy root overexpression strain is obtained on the basis of a hairy root genetic transformation system of a chassis cell, that is, the chassis cell line is obtained. Using the chassis cell line can achieve the effect of efficient and directed synthesis of cyanidin by means of gene overexpression, and the content thereof is relatively high, thereby remarkably improving the purity of extracted anthocyanins. The chassis cell line has the advantages of short growth cycle and simple culture mode, and is suitable for industrial mass production.

This invention belongs to the field of active ingredient extraction, specifically relating to a purslane extract screened using bioaffinity ultrafiltration, its preparation method, and its applications. This invention utilizes bioaffinity ultrafiltration to rapidly capture active small molecules that specifically bind to XOD from purslane extract, significantly shortening the screening cycle for natural product XOD inhibitors. Furthermore, the screened compounds have been verified in vitro to possess XOD inhibitory activity. The method exhibits high sensitivity and good reproducibility. This invention is the first to discover and clarify Ophiogonanone A, Cimidahurinine, and Cyanidin-3- O γ-glucoside is a natural XOD inhibitor found in purslane, providing a new natural candidate molecule for drugs that lower uric acid or treat gout.

The invention relates to a novel anthocyanidin O-methyltransferase (lmg 13579), a coding gene of the novel anthocyanidin O-methyltransferase and application of the novel anthocyanidin O-methyltransferase in catalyzing cyanidin-3-O-glucoside to generate paeoniflorin-3-O-glucoside. Gene cloning, prokaryotic expression and in-vitro enzyme activity verification prove that the enzyme can specifically catalyze methylation modification of anthocyanin B ring 3 '-hydroxyl sites. High-efficiency soluble expression is realized by using an escherichia coli heterologous expression system, and high-purity protein is obtained after His / GST double-tag purification. An in-vitro enzymatic reaction shows that after the reaction is carried out for 1 hour under the condition of 30 DEG C, a remarkable characteristic substance peak appears, and the substance is identified as paeoniflorin-3-O-glucoside (RT = 2.78) through LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry). The discovery of the enzyme can improve the problems of low catalytic efficiency, low substrate binding specificity, unclear catalytic sites and the like of anthocyanin O-methyltransferase, and the molecular efficiency and specificity of synthesis of paeoniflorin-3-O-glucoside are improved by obtaining zymoprotein with definite functions and optimizing the catalytic process of anthocyanin methylation.

The application provides a method for determining six natural pigments in cosmetics by high performance liquid chromatography. The steps are as follows: preparing a standard stock solution: accurately weighing curcumin, shikonin, lutein, cochineal, astaxanthin and cyanidin standard, and preparing a standard stock solution; preparing a mixed standard working solution: mixing the standard according to a certain mass ratio, and preparing a mixed standard working solution; preparing a sample solution to be determined: the natural pigments in the sample are fully extracted by vortex, ultrasonic and centrifugation; sample on machine: the treated solution is filtered through a filter membrane and is determined by high performance liquid chromatography; and result analysis: the six natural pigments are qualitatively determined by retention time, and the natural pigments are quantitatively determined by a standard curve. The application can simultaneously detect a plurality of natural pigments, is simple to operate, has high sensitivity, is suitable for detecting multiple batches of cosmetics, and provides technical support for product quality control in the cosmetic industry.

The invention discloses a sweet taste enhancing and taste improving composition based on procyanidine B2 and cyanidin-3-O-glucoside, and the composition is prepared from the following raw materials in parts by weight: 0.05 to 0.07 part of aspartame or 0.01 to 0.03 part of sucralose, 0.02 to 0.03 part of cyanidin-3-O-glucoside, 0.02 to 0.03 part of procyanidine B2, 1.5 to 2.5 parts of erythritol and the balance of water. According to the invention, a double-polyphenol synergistic sweetening system is innovatively constructed, a sweetness signal is enhanced by cyanidin-3-O-glucoside, a sweetening agent is stabilized by procyanidine B2, sweetness perception is prolonged, bad aftertaste is neutralized by matching with erythritol, mellow feeling is supplemented, and an integrated scheme of double-polyphenol sweetening and sugar alcohol regulation is formed. Compared with theoretical superposition, the composition has the advantages that the sweetness is improved by 45.6%, the synergistic increasing rate reaches 28.9%, bad flavors such as metal flavor and bitter taste are remarkably inhibited, the aftertaste residue is less than or equal to 2s, the taste is close to that of cane sugar, and the composition can be widely applied to low-sugar food, takes low sugar and health into account, and has important industrial application value.

The invention discloses a rose anthocyanin extract capable of delaying senescence as well as a preparation method and application thereof, and relates to the technical field of natural product extraction. According to the method, the rose anthocyanin extract with high purity and high activity is obtained sequentially through supercritical carbon dioxide extraction and degreasing, citric acid aqueous solution extraction, chitosan flocculation and clarification, multi-stage membrane separation and purification and freeze drying, the main active ingredient of the anthocyanin extract is cyanidin 3, 5-diglucoside, and the content of the cyanidin 3, 5-diglucoside in the rose anthocyanin extract is 1-5%. The compound has the effects of resisting oxidation, resisting light aging and delaying senescence, and can be widely applied to cosmetics, health-care foods and pharmaceutical preparations. By optimizing the extraction method, the extraction rate and purity of the rose anthocyanin can be greatly improved, the extraction method is superior to a traditional extraction method, the extraction process is simple, the extraction reagent is easy to obtain, pollution and blocking pressure to a membrane treatment system can be reduced, the service life of a membrane is prolonged, and large-scale industrial production and use are facilitated.

The invention relates to the technical field of microbial fermentation, and particularly discloses a screening method and application of color-protecting yeast based on anthocyanin. The method for efficiently screening the color-protecting yeast comprises the following steps: firstly, carrying out enrichment culture on a natural flora on the surface of a fruit in an optimal ripening stage, then carrying out separation and purification operation on the obtained natural flora, and sequentially carrying out a series of technical means such as tolerance testing, a microwell plate fermentation technology and spectral characterization analysis, so as to obtain the color-protecting yeast. The color protection performance of the separated strain is systematically evaluated, and the grade classification of the color protection capability of the yeast strain is realized. The invention also provides an application of the high-efficiency color-protecting yeast strain, the strain shows excellent color-protecting efficiency in a mulberry wine fermentation system, the total anthocyanin retention rate and the cyanidin-3-O-glucoside retention rate are obviously higher than those of commercial yeast strains, and the high-efficiency color-protecting yeast strain has good color-protecting effect. The key problems that in the prior art, a color protection yeast strain screening method is deficient, and the color protection efficiency is insufficient are effectively solved.