22 results about "Lonicera caerulea" patented technology

According to the preparation method disclosed by the invention, the whole fruit juice of the lonicera caerulea (which retains fruit residues) is fermented by virtue of the plant lactobacillus KLDS1.0391, KLDS1.0320 or KLDS1.0368, so that the xanthine oxidase (XO) inhibition activity is remarkably improved. After fermentation, the XO inhibition rate is increased to 48-50.87% (the content of unfermented juice is 31.21%), the total phenol content is increased by 15-24.3%, and the contents of main XO inhibitors in the lonicera caerulea: cyanidin-3-O-glucoside (IC50 = 115.28 [mu] M), cyanidin-3, 5-diglucoside (IC50 = 116.34 [mu] M), peonidin-3-glucoside (IC50 = 105.34 [mu] M), quercetin (IC50 = 88.75 [mu] M), catechin (IC50 = 110 [mu] M) and caffeoylquinic acid (IC50 = 250.35 [mu] M) are remarkably increased. Wherein the quercetin content is increased by 73.8% at most (KLDS1.0320 strain fermentation is performed for 24 hours), and the XO inhibition rate is increased from 31.21% to 50.87%. Technical support is provided for development of a natural XO inhibitor and a functional fermented beverage, so that a new thought is provided for functional fermented juice with lonicera caerulea as a key component.

A lonicera caerulea fruit picking clamp with a crab-claw-shaped jaw structure belongs to the field of lonicera caerulea fruit picking tools and comprises a positioning clamp arm and a cutting clamp arm, the positioning clamp arm and the cutting clamp arm are respectively provided with a control handle, the control handles are mutually crossed and hinged to form a picking clamp body, and the front end of the positioning clamp arm is provided with a bearing frame and is correspondingly provided with a storage channel. The bearing frame is of a crab claw-shaped jaw structure with a bearing face and a V-shaped jaw, a shearing blade is arranged on the edge of one side of the V-shaped jaw, the bearing frame and the containing channel are arranged correspondingly, a cutting blade is arranged at the front end of the shearing pliers arm, and a lateral blade corresponding to the shearing blade structure on the edge of one side of the V-shaped jaw is arranged on the cutting blade. The V-shaped jaw can be matched with the bearing frame to form a shearing structure, the V-shaped jaw is matched with the cutting blade to shear off fruit stems to achieve picking, fruits do not bear any external force in the harvesting process, the harvesting quality can be fully guaranteed, the picking difficulty can be lowered in production and use, the picking efficiency is improved, the labor intensity is relieved, and potential safety hazards are reduced.

The invention relates to the technical field of fruit and vegetable preservation, and provides preparation and application of a spraying type nutritional preservative for lonicera caerulea before harvesting. Wherein each kilogram of the nutrient preservative contains 0.012 to 0.035 g of melatonin, 0.8 to 1.5 g of epsilon-polylysine, 0.8 to 1.2 g of water-soluble perilla leaf superfine powder, 1.6 to 2.0 g of food-grade refined pure colorless bamboo vinegar, 0.8 to 1.0 g of lactic acid small molecule chelated calcium, 0.6 to 1.0 g of food-grade small molecule water-soluble oligosaccharides and the balance of water. The fruit surface is sprayed 0-3 days before harvesting, harvesting can be performed after the fruit surface is naturally dried, the operation method is simple and easy to implement and suitable for large-scale industrial application, the normal-temperature preservation period of the harvested lonicera caerulea is 7 days or above, precise temperature control storage is 112 days or above, the mildew phenomenon of the lonicera caerulea in the later storage period can be delayed, and the hardness, the fruit frost coverage rate and the freshness of the fruits can be effectively kept.

The utility model relates to the technical field of fertilization and irrigation, and discloses precise fertilization and irrigation integrated equipment for planting lonicera caerulea, which comprises a bottom plate, a plurality of fertilization and irrigation devices and a plurality of fertilization and irrigation devices, the irrigation part is arranged at the bottom of the bottom plate; the opening and closing part is arranged at the top of the bottom plate; and the moving wheels are mounted at the bottom of the bottom plate. A handle is held by hand, so that a bottom plate is driven to move under the action of moving wheels, an electric telescopic seat is driven, a connecting block vertically slides down, a ploughing cutter turns soil, materials fall into a material storage box under the action of an opening and closing part, and the materials are uniformly sprayed out under the action of a spraying head; due to the fact that the spraying head for spraying water and the spraying head for throwing the fertilizer are located on the same vertical plane, the purpose of precisely spraying the thrown fertilizer is achieved, and due to the fact that the ploughing cutter and the spraying heads are located on the same vertical plane, the purpose of precisely fertilizing is achieved.

The invention provides a functional beverage and an application thereof in anti-hypoxia. The functional beverage disclosed by the invention is obtained by compounding a lonicera caerulea extracting solution, an acanthopanax extracting solution and a schisandra chinensis extracting solution according to a specific ratio. The functional beverage can prolong the survival time of an organism in a closed hypoxia environment, relieve lung edema or hyperemia symptoms caused by hypoxia, relieve lung pathological changes caused by hypoxia, effectively reduce the levels of protein kinase B alpha, vascular endothelial cell growth factors and hypoxia-inducible factor-1 alpha in lungs, and improve the immunity of the lungs. Therefore, oxidative stress injury can be inhibited, the anti-hypoxia capability of an organism is improved, and a good anti-hypoxia effect is achieved. In addition, the functional beverage also has good biological safety, easily acceptable flavor and stable state, and is suitable for industrial popularization.

The invention provides detection liquid sample sampling equipment for lonicera caerulea gene sequencing, and relates to the technical field of sample sampling. The device comprises a base, a supporting column, a sampling arm, a control panel and a cleaning device, the control panel is provided with operation buttons and a display screen which are used for controlling a sampling head to move, a suction device to work and the cleaning device to work, automatic sampling and cleaning operation of the device is achieved through a preset program, and the sampling arm is provided with the sampling head capable of moving up and down. The sampling head is connected with a suction device for extracting a liquid sample, and a sample placing table for placing a lonicera caerulea liquid sample container is arranged on the base. By means of automatic control and precise structural design, high-precision liquid sample sampling can be achieved, cross contamination of samples is effectively avoided, and the sample quality is improved; meanwhile, due to the replaceable sampling head, the flexible driving mechanism and the automatic cleaning device, the strict requirements of lonicera caerulea gene sequencing on high-quality samples are met.

According to the method disclosed by the invention, the contents of cyanidin-3-O-glucoside, delphinidin-3-O-glucoside and quercetin-3-glucoside in the fresh lonicera caerulea fruits are extracted and determined by applying a ternary eutectic solvent and combining a microwave extraction method. The monomers extracted by the method disclosed by the invention are relatively high in content and very stable. A single factor test carries out orthogonal optimization on selection of the type, the molar ratio, the water content, the solid-to-liquid ratio, the microwave extraction temperature and the microwave extraction time of the ternary eutectic solvent, and an orthogonal test shows that when betaine-glycerol-glucose (Bet-Gly-Glu) with the molar ratio of 1: 3: 1, the water content is 40%, the liquid-to-solid ratio is 20: 1, the extraction temperature is 50 DEG C and the extraction time is 15 min, the content of the glycine betaine is 40%, and the content of the glycine betaine is 40%. The content of the extracted cyanidin-3-O-glucoside is the highest, and the content of the extracted cyanidin-3-O-glucoside is 12.30 mg / g to 12.35 mg / g; the content of the delphinidin-3-O-glucoside is 12.60 mg / g to 12.65 mg / g; the content of the quercetin-3-glucoside is 0.55 mg / g to 0.60 mg / g. The extracted fresh lonicera caerulea fruit pulp extracting solution has relatively high antioxidant and hypoglycemic activity, and can be applied to the fields of antioxidant and hypoglycemic.

The application discloses a method for extracting and purifying total iridoid glycosides in Lonicera caerulea and application thereof, and comprises the following steps: (1) extraction: a certain amount of Lonicera caerulea powder is weighed, methanol solution is added, and heating is performed to 40-60 DEG C, ultrasonic-assisted extraction is performed for 20-40 min, filtration is performed, methanol is recovered by reduced pressure concentration, and Lonicera caerulea extract is obtained; (2) purification: the Lonicera caerulea extract is subjected to macroporous adsorption resin column chromatography, elution is performed with ethanol, ethanol is recovered by reduced pressure concentration, and total iridoid glycosides are obtained. The application can effectively improve the extraction amount of total iridoid glycosides in Lonicera caerulea and the purity of the extract.

The invention discloses lonicera caerulea compound powder as well as a preparation method and application thereof, and relates to lonicera caerulea compound powder as well as a preparation method and application thereof. The objective of the invention is to solve the problem of myopia caused by long-time use of electronic products, few outdoor activities and short eye distance. Every 3 g of the lonicera caerulea compound powder contains 100 mg of lutein ester microcapsule powder with the specification of 10%, 20 mg of zeaxanthin microcapsule powder with the specification of 10%, 450 mg of lycium barbarum polysaccharide with the specification of 80% and 2430 mg of lonicera caerulea freeze-dried powder, and the 2430 mg of lonicera caerulea freeze-dried powder contains 1780 mg of maltodextrin. The lonicera caerulea compound powder is used for dispelling the effects of alcohol, removing DPPH, ABTS and O2-free radicals and resisting lipid peroxidation. The lonicera caerulea compound powder has strong antioxidant capacity, so that good double-effect effects of protecting eyes, protecting liver and dispelling the effects of alcohol are achieved. The invention belongs to the technical field of prevention and control of myopia and hangover alleviating.

The invention relates to the technical field of spectral quantitative analysis, in particular to a spectral quantitative analysis method and system for active ingredients of lonicera caerulea based on deep learning, and the method comprises the following steps: material selection and cleaning, sampling environment setting, sample sampling, active ingredient sample spectrum determination and template area determination. The system comprises a base, a detection shell is installed at the upper end of the base, and a lifting mechanism is jointly installed in the base and the detection shell. The lonicera caerulea can be fully processed, the stability of the environment during sampling is ensured, the situation that the sampling quality is affected due to changes of the external environment condition is avoided, meanwhile, the spectrum information of active ingredients in the lonicera caerulea can be accurately obtained, and then through spectrum sampling on the surface of the lonicera caerulea, the quality of the active ingredients in the lonicera caerulea is improved. The comparison of the sampling spectrum information and the spectrum information of different active components is fully realized through an algorithm so as to analyze the active components; meanwhile, the structure scheme of the sampling analysis system is also shown.

The invention discloses a lonicera caerulea anthocyanin delivery system constructed based on pine nut meal protein and a preparation method of the lonicera caerulea anthocyanin delivery system, and relates to a lonicera caerulea anthocyanin delivery system and a preparation method thereof. The invention aims to solve the technical problems of poor encapsulation, poor bioaccessibility and poor stability after redissolution of the existing anthocyanin delivery platform. According to the lonicera caerulea anthocyanin delivery system constructed based on the pine nut meal protein, lonicera caerulea anthocyanin is dispersed in an emulsion network on the surface of a ternary covalent compound, and the ternary covalent compound is constructed through free radical grafting and Maillard reaction of the pine nut meal protein, tannic acid and maltodextrin. The preparation method comprises the following steps: preparing a ternary covalent complex from pine nut meal protein, tannic acid and maltodextrin, adding a lonicera caerulea anthocyanin solution, mixing, filtering and sterilizing or freeze-drying to obtain the lonicera caerulea anthocyanin delivery system. The bioaccessibility of anthocyanin in the system is 22.88%, and the system can be used in the field of food processing of powder, beverages, gel, soft sweets and the like.

The invention relates to a cultivation technology of wild vegetables and fruit trees, in particular to a compound planting method of ligusticum and lonicera caerulea, which comprises the following steps: on the premise of uniformly making a foundation bed, planting one bed of lonicera caerulea every other bed, and planting ligusticum between plants of an empty foundation bed and a lonicera caerulea planting foundation bed. The method comprises the steps that the height of a foundation bed is 25 + / -2 cm, and the width of the foundation bed is 90 + / -2 cm; footpaths with the width of 55-65 cm are reserved on the two sides of the foundation bed respectively, compound planting of ligusticum and lonicera caerulea is conducted on each first bed, and only ligusticum is planted on each second bed. Lonicera caerulea is planted according to the plant spacing of 2.5 m-3. 5m * the line spacing of 2.5 m-3. 5m. The ligusticum is planted according to the plant row spacing of 10 cm * 10 cm; when ligusticum is planted in the lonicera caerulea seedbed, lonicera caerulea planting holes with the radius larger than or equal to 50 cm should be reserved. According to the method, ligusticum and lonicera caerulea are planted on the same land parcel at the same time, due to the fact that the requirements for water, fertilizer, pH values and the like are similar, unified management can be achieved, and a management scheme does not need to be independently designed. And the land utilization rate can be increased, the use efficiency of water, fertilizer and the like can be increased, and the economic output value per unit area can be increased.

The invention relates to the technical field of lonicera caerulea breeding. The invention provides an artificial breeding method of lonicera caerulea in a cold region, which comprises the following steps: (1) cutting annual branches of lonicera caerulea, covering with a preservation matrix, and preserving at low temperature; (2) taking out the branches stored at low temperature, cutting the branches into cutting slips, and disinfecting the cutting slips for later use; (3) turf, rice husks, sand and soil are mixed to obtain a seedling raising substrate, and then the cutting slips are inserted into the seedling raising substrate; (4) performing seedling stage management on the cutting slips; and (5) after seedling stage management is finished, transplanting the seedlings into a finished product seedling matrix, and continuing to cultivate to obtain finished lonicera caerulea seedlings. The lonicera caerulea artificial breeding method suitable for the cold region is obtained through selection of seedling branches and matching of matrixes, the breeding time is short, a large number of lonicera caerulea seedlings can be rapidly obtained through the method, and large-scale popularization of the seedlings is facilitated.

The invention discloses a composite plant enzyme beverage as well as a preparation method and application thereof, and relates to the technical field of food processing. The composite plant enzyme beverage is prepared from low-sugar alcohol effect dispelling enzyme liquid, ganoderma lucidum soaking liquid, radix puerariae, lophatherum gracile, lotus seeds, folium mori, fructus gardeniae, fructus momordicae, radix polygonati officinalis, liquorice and water, and the low-sugar alcohol effect dispelling enzyme liquid is prepared from triquetrous tadehagi herb, cichorium intybus, radix fici simplicissimae, lonicera caerulea, mulberries, thunberg fritillary bulbs, kelp, cyclocarya paliurus leaves, trametes robiniophila, pleurotus tuber-regium polysaccharide and water. The low-sugar hangover-alleviating enzyme liquid and the composite plant enzyme beverage prepared by the invention are suitable for drunkenness diabetics and obesity patients to drink, and are good in hangover-alleviating effect, safe and non-toxic, and the prepared raw materials are easy to obtain.

The utility model relates to the technical field of spectral quantitative analysis, in particular to a spectral quantitative analysis system for active ingredients of lonicera caerulea based on deep learning, which comprises a base, a detection shell is mounted at the upper end of the base, a lifting mechanism is jointly mounted in the base and the detection shell, a supporting plate piece is mounted on the lifting mechanism, and the supporting plate piece is mounted on the detection shell. A mounting box is arranged on one side of the detection shell in a penetrating manner, a linkage mechanism is mounted in the mounting box, an extension rod piece and a mounting frame are arranged on the linkage mechanism, and the extension rod piece is connected with the supporting plate piece. According to the system, shooting and hyperspectral image acquisition operation in a closed environment can be realized, the influence of an external environment on image acquisition can be fully avoided, meanwhile, circulating acquisition operation can be realized, and the acquisition efficiency and quality are improved.

The method comprises the following steps: (1) taking a certain amount of lonicera caerulea leaves, drying the lonicera caerulea leaves at 60 DEG C, grinding the lonicera caerulea leaves, and sieving the lonicera caerulea leaves; (2) adding an ethanol aqueous solution with the concentration of 50% into the lonicera caerulea leaf powder obtained in the step (1) according to the material-liquid ratio of 1: 50 (g / ml), soaking for 60 minutes, extracting for 4 times under the condition of 300W power in combination with an ultrasonic method, combining extracting solutions, and performing suction filtration; (3) performing reduced pressure distillation on the ethanol extracting solution obtained in the step (2), and fixing the volume to 100ml; (4) centrifuging the solution obtained in the step (3), carrying out secondary suction filtration, and fixing the volume to 100 ml; and (5) performing reduced pressure distillation on the solution obtained in the step (4) to obtain paste, thereby obtaining the lonicera caerulea leaf crude extract. The method disclosed by the invention is simple in steps, convenient to operate, short in extraction time, high in extraction rate and few in impurities.

The invention discloses lonicera caerulea processed cheese with an anti-oxidation effect and a preparation method of the lonicera caerulea processed cheese, and belongs to the technical field of dairy product processing. The antioxidant effect of the processed cheese is remarkably improved by adding the lonicera caerulea pulp, experimental data shows that along with increase of the addition amount of the pulp, the DPPH free radical scavenging rate can be increased, the ABTS free radical scavenging rate can reach 50% or above, it is indicated that ingredients such as anthocyanin in lonicera caerulea effectively endow the product with antioxidant activity, and gelatin (1-3%) does not directly affect the antioxidant activity and does not directly affect the antioxidant activity of the lonicera caerulea. The retention of active ingredients is indirectly supported by stabilizing the texture; sensory evaluation shows that the prepared lonicera caerulea processed cheese with the anti-oxidation effect presents a light purple appearance, the fruit fragrance and the milk fragrance are naturally fused, the texture is uniform, and the hardness is moderate.

The invention discloses a lonicera caerulea polyphenol extraction method based on a deep-eutectic solvent and application of lonicera caerulea polyphenol extraction method, and belongs to the technical field of plant extraction. A method for extracting lonicera caerulea polyphenol by using a deep-eutectic solvent comprises the following steps: S1, freeze-drying fresh lonicera caerulea, and crushing to obtain lonicera caerulea freeze-dried powder; s2, uniformly mixing a hydrogen bond acceptor and a hydrogen bond donor reagent, heating and stirring until transparent and uniform liquid is formed, and naturally cooling to room temperature to obtain DES; s3, mixing the lonicera caerulea raw material with DES according to a ratio of 1: (10-50) g / mL, putting the mixture into an ultrasonic instrument, extracting at set temperature, time and ultrasonic power, and centrifuging to obtain supernate, namely a lonicera caerulea polyphenol extracting solution. The yield of the lonicera caerulea polyphenol extracted by the method is obviously higher than that of the lonicera caerulea polyphenol extracted by a traditional water solvent and an alcohol solvent, and the obtained extract has excellent antioxidant activity and antithrombotic activity.

The invention relates to the technical field of microorganisms and fermentation engineering, and particularly discloses a Pichia kluyveri strain H421, and the preservation number of the Pichia kluyveri strain H421 is CGMCC (China General Microbiological Culture Collection Center) NO.34649. The bacterial strain H421, commercial saccharomyces cerevisiae D254 and schizosaccharomyces pombe CICC 32484 are sequentially inoculated and fermented, the aroma of the brewed lonicera caerulea fruit wine is dominated by isoamyl acetate and phenethyl acetate, the brewed lonicera caerulea fruit wine is rich in various esters such as ethyl caprylate, ethyl caprate and diethyl succinate, and the characteristic flower and fruit aroma is jointly formed. The strain H421 has excellent alcohol resistance, acid resistance and SO2 resistance, and can adapt to the fermentation environment of the lonicera caerulea fruit wine. By adopting a multi-strain sequential inoculation fermentation method including the strain, the content of malic acid in the lonicera caerulea fruit wine can be remarkably reduced, and the variety and content of aroma substances are greatly increased, so that the problems of strong acerbity and single aroma of the lonicera caerulea fruit wine are effectively improved.

The invention discloses novel young processed cheese and a preparation method thereof, and further improves the taste and nutrition of cheese products in the food field. The preparation method comprises the following steps: (1) weighing related raw materials by weight; and (2) preparing the lonicera caerulea into the lonicera caerulea jam. (3) cutting and crushing natural cheese, adding water, heating for melting, decalcifying, adjusting the pH value, adding related raw materials into the natural cheese, and uniformly mixing; and (4) dissolving soybean protein isolate and glycerol in distilled water, stirring at 85 DEG C, cooling to room temperature, adding anthocyanin to prepare an anthocyanin / SPI composite film, and covering the surface of the prepared reproduced cheese with the prepared edible film. The product has the advantages of high-quality raw materials, scientific and nutritional formula and mature processing technology, and is innovated in the forms of flavor, appearance and the like in the direction of diversification, health and comprehensive nutrition. The market prospect is good, and the development space is very large. On the basis of a core basic product, the functions of the product are extended, and powerful support is provided for development of the functional food market.

The utility model relates to the technical field of molecular distillation, in particular to a lonicera caerulea anthocyanin molecular distillation device which comprises a tank body, a blanking frame, a cooling frame and a heating seat, the blanking frame is fixedly connected to the top surface of the tank body, a conical ring is fixedly connected to the bottom of the inner side surface of the blanking frame, and a conical seat is fixedly connected to the inner top surface of the blanking frame. A partition plate is arranged between the conical ring and the conical seat, and the cooling frame is arranged in the tank body. According to the device, the partition plate is arranged between the conical ring and the conical base, after a lonicera caerulea anthocyanin solution is conveyed into the discharging frame, the solution can flow to the inner wall of the tank body and the side face of the heating base, light molecules can be condensed on the inner surface and the outer surface of the cooling frame, and heavy molecules can continue to flow downwards along with the solution; the solution moves along the inner wall of the tank body and the side wall of the heating seat, so that the evaporation area is relatively large, the distillation efficiency is favorably improved, and the lonicera caerulea anthocyanin can be quickly extracted.