The invention discloses a method for cultivating potato
virus-free seedlings, relates to the
virus elimination and
tissue culture techniques of plants, and belongs to the field of agricultural
seedling breeding. The method is characterized by comprising the following steps of: transferring
virus-free potato
tissue culture seedlings into a MS culture medium to culture, wherein a
bacteriostatic agent and
sugar are added into the MS culture medium; and after the culturing is completed, soaking the seedlings into a CIP improved rooting agent, and carrying out cuttage planting and
nutrient solution applying on the seedlings, and after the seedlings are mature, harvesting the seedlings. The method disclosed by the invention has the beneficial effects that through cultivating virus-free seedlings in a special culture medium, the
contamination rate in the culture medium is reduced; then, the
plant seedlings cultivated in the culture medium are subjected to cuttage, and before cuttage, the cuttage seedlings are placed in the CIP improved rooting agent, and
nutrient solutions N, P and K are applied, so that the survival of virus-free seedlings is ensured, the potato growth amount and the expansion of tubers are promoted, thereby improving the yield; and the single-
plant potato growth amount of breeder seeds is improved, so that the production cost of breeder seeds is reduced by 25%, thereby providing a reliable
technical support for the industrialized development of potatoes.