Actinidia arguta tissue culturing method
A technology of kiwi fruit and tissue culture, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve problems such as the intensified contradiction between supply and demand of seedlings, the unfinished promotion and popularization of fine varieties, and the impact on the development of the deep processing industry of kiwi fruit , to achieve the effect of stable genetic traits
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[0008] The shoots germinated from the dormant branches of the kiwifruit jujube after hydroponics are used as explants, which are specifically composed of the following four steps:
[0009] (1) Explant sterilization firstly adopts conventional method to wash the sprouts extracted by hydroponics with washing powder solution, then sterilize with 0.1% mercuric chloride for 5 minutes, and soak in sterile water for 40 minutes;
[0010] (2) Inoculation Under sterile conditions, the shoots were inoculated on the primary medium, which was supplemented with 0.5 mg / L of 6-benzyl adenine (6-BA), 1 mg of MS conventional medium / L of gibberellin (GA 3 ), PH 5.8, and basal seedlings were cultivated. The cultivation temperature is 25±2℃, the light period is 12h / d, and the light intensity is 2000lux. After 20 days of cultivation, the seedling height is about 3-4cm, and the next stage is started;
[0011] (3) Subsequent proliferation The basal seedlings were transferred to the subculture prol...
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