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Actinidia arguta tissue culturing method

A technology of kiwi fruit and tissue culture, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve problems such as the intensified contradiction between supply and demand of seedlings, the unfinished promotion and popularization of fine varieties, and the impact on the development of the deep processing industry of kiwi fruit , to achieve the effect of stable genetic traits

Inactive Publication Date: 2009-11-11
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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Problems solved by technology

However, the tissue culture system of Actinidia jujube has not been established, the promotion and popularization of fine varieties has not been completed, and the contradiction between supply and demand of seedlings has become increasingly serious, which has seriously affected the development of the deep processing industry of Actinidia jujube.

Method used

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  • Actinidia arguta tissue culturing method
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  • Actinidia arguta tissue culturing method

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Embodiment Construction

[0008] The twigs germinated from the dormant branches of Actinidia jujube as explants after hydroponics, specifically consisted of the following four steps:

[0009] (1) The explants are sterilized firstly by conventional method to wash the tender shoots taken out by hydroponics with washing powder solution, then sterilize with 0.1% mercuric chloride for 5 minutes, and soak in sterile water for 40 minutes;

[0010] (2) Inoculation The shoots were inoculated on the primary culture medium under sterile conditions, which added 0.5 mg / L of 6-benzyl adenine (6-BA), 1 mg / L gibberellin (GA 3 ), PH 5.8, to cultivate basic seedlings. The cultivation temperature is 25±2°C, the photoperiod is 12h / d, and the light intensity is 2000lux. After 20 days of cultivation, the height of the seedlings is about 3-4cm, and the next stage begins;

[0011] (3) Subculture propagation The basal seedlings were transferred to the subculture propagation medium for proliferation culture. MS+6-BA (0.5mg / ...

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Abstract

The invention belongs to the field of plant tissue culturing technologies and forest cultivation and relates to a high-efficiency actinidia arguta propagation method. The method comprises four stages of explant sterilization, inoculation, multiplication and rootage. The method solves the important technical problems of microbody quick propagation and root induction of the actinidia arguta, achieves the aims of efficiently inducing explants to separate and propagate and guaranteeing that seedlings survive with high quality, preserves all good characters of a matrix, and ensures stable hereditary character. The method can form large qualities of good test-tube seedlings within a short period and is suitable for large-scale and industrial production.

Description

technical field [0001] The invention relates to a vegetative propagation method of berries in Northeast China, that is, a tissue culture method of kiwi fruit. The invention belongs to the field of plant tissue culture technology and forest cultivation. Background technique [0002] Actinidia jujube fruit is a rare, natural, green edible berry, because of its potential nutritional value and commercial value, it is a rare, pure natural, green edible berry in the world, and it is extremely promising for development. One of the third-generation fruits in the foreground. However, due to the unplanned and plundering picking, the resources of kiwifruit were seriously damaged, the natural repair ability was reduced, the reserves and recoverable quantities were sharply reduced, and the contradiction between supply and demand was increasingly aggravated. [0003] The seedling raising techniques of Actinidia jujube in my country are mainly vegetative propagation (i.e. branching, laye...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 田新华邢亚娟兰世波李海霞周志军
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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