Cultivation method for inducing scutellaria baicalensis hairy root based on agrobacterium rhizogenes infection

Abstract

The invention discloses a cultivation method for inducing scutellaria baicalensis hairy root based on agrobacterium rhizogenes infection, which uses an agrobacterium rhizogenes bacteria liquid to infect root of a scutellaria baicalensis explant and induces the scutellaria baicalensis explant to form a hairy root, the scutellaria baicalensis hairy root presents yellow hairy on a MS solid medium, so that scutelloside can be stably synthesized, an ITS sequencing is performed and the hairy root can be identified as the scutellaria baicalensis hairy root of a scutellaria. Then seed selection is performed for further in a seed selection medium and a cultivation condition can be optimized to select lines which raise the content of active ingredient scutelloside used for hairy root traditional Chinese medicines. Hairy root can be obtained through scutellaria of a scutellaria introduced by agrobacterium rhizogenes, wherein the inductivity can reach 10 - 40%, the obtained scutellaria baicalensis hairy root presents yellow hairy which is capable of stably synthesizing scutelloside. According to the invention, the scutellaria baicalensis hairy root of a scutellaria is cultivated on the optimized medium, the growth is rapid and the biomass can be increased for 23 -35 times in 30 - 35 days, and the cultivation is performed under the dark conditions. The invention has the characteristics of low energy consumption and low cost, which is suitable for industrialization production.

Description

technical field

[0001] The invention belongs to the technical field of artificial cultivation of scutellariae, and relates to a cultivation method for inducing hairy roots of scutellaria baicalensis based on Agrobacterium rhizogenes infection. Background technique

[0002] Scutellaria baicalensis Georgi. (Scutellaria baicalensis Georgi.) is a plant of the Labiatae family and is a commonly used traditional Chinese medicine. It is cold in nature and bitter in taste. It belongs to the lung, heart, liver, gallbladder, and large intestine meridians. Scutellaria baicalensis is a perennial herb, which likes mild climate and is resistant to cold and drought. It is mainly distributed in Hebei, Liaoning, Shaanxi, Shandong, Inner Mongolia and other places. The main components are baicalin, baicalein, wogonin, and wogonin. With the in-depth international research on Scutellariae, the main component of Scutellariae baicalin has good effects in scavenging oxygen free radicals, reducing t...

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