107 results about "Rhizogene" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to an agrobacterium rhizogenes-mediated visualized genetic transformation method for cucurbitaceae plants, which comprises the step of transfecting the cucurbitaceae plants by using agrobacterium rhizogenes containing recombinant plasmids, and the recombinant plasmids are expression cassettes containing Ruby reporter genes. A visual agrobacterium rhizogenes-mediated transformation system of the cucurbitaceae plants is established by utilizing the RUBY gene, professional equipment or chemical treatment is not needed, red hairy roots can be visually screened to identify and induce the positive plants, and the method is simple, rapid and efficient and can be used for identifying and inducing the positive plants of the cucurbitaceae plants such as bottle gourds and the like. And the gene is transferred into an exogenous gene or is subjected to gene editing on the cucurbitaceae plant, so that the gene has important significance.

The invention relates to the field of asexual propagation of avocados, in particular to a method for inducing rooting of avocado cutting slips by utilizing agrobacterium rhizogenes. The method comprises the following steps: under open experimental conditions, taking current-year-old semi-lignified shoots of grafting plant scions of persea americana L. of 2-3 years old as experimental materials, taking high-temperature sterilized coco coir as a cutting medium, carrying out vacuum infiltration infection on the shoots in an agrobacterium rhizogenes K599 infection solution for 20 minutes, infecting wounds of seedlings, inducing rooting, and carrying out rooting. A system for inducing avocado cutting rooting by using agrobacterium rhizogenes is established. According to the method, the technical problem that the avocado cutting slips are difficult to root is solved, and the technical effect of rapidly breeding the avocado clonal rootstock seedlings is achieved. According to the method, clonal breeding, popularization and application of the excellent stock variety can be realized, a more convenient and efficient way is provided for breeding, popularization and application of the excellent persea americana stock variety, and the method has important significance for breeding, popularization and application of the excellent persea americana stock.

The invention relates to the technical field of biology, and particularly discloses an agrobacterium rhizogenes mediated broad bean hairy root induction method without tissue culture, which comprises the following steps: germination of broad bean seeds, transformation of agrobacterium, infection of explants, co-culture of the explants and identification of transgenic broad bean hairy roots. According to the broad bean hairy root induction method provided by the invention, transgenic hairy roots can be obtained only in 2-4 weeks, the method is simple to operate, and tedious sterile operation is avoided; besides, the recombinant agrobacterium rhizogenes of a betaine biosynthesis system RUBY are utilized to infect the broad bean hypocotyl and then culture, so that the broad bean hairy root induction method is successfully established, and the transgenic hairy root can be clearly observed by naked eyes without special equipment or chemical treatment.

The invention provides an agrobacterium rhizogenes-mediated forsythia suspensa hairy root genetic transformation method and application thereof, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: introducing an expression vector into agrobacterium rhizogenes, and mixing with an infection solution to prepare an agrobacterium rhizogenes infection solution; the forsythia suspensa stem is put into the agrobacterium rhizogenes infection liquid to be infected for 0.5-1 h through a vacuum infiltration method, and the infected forsythia suspensa stem is cut into a matrix to be subjected to dark culture and then continues to grow till hairy roots are generated; the OD600 value of the agrobacterium rhizogenes infection liquid is 0.5 to 1.0. The method is suitable for an agrobacterium rhizogenes mediated genetic transformation system of the fructus forsythiae, and the system provides technical support for functional gene research, medicinal component analysis and molecular breeding of the fructus forsythiae, and has important significance for promoting genetic improvement and resource utilization of the fructus forsythiae.

The invention specifically discloses a salix cheilophila in-situ genetic transformation method independent of a tissue culture system, and belongs to the field of plant genetic engineering. According to the method, semi-lignified branches of salix cheilophila are taken as receptors, vacuumizing treatment is performed by utilizing agrobacterium rhizogenes infection liquid, hairy roots are directly induced under a water culture condition after short-term dark culture, and positive identification is performed through reporter genes. According to the method, tedious sterile tissue culture operation is completely avoided, the transformation period is shortened to about 22 days, and the technical threshold and the time cost are remarkably reduced. By adopting the system, the positive rate can reach 54.33% when the pBI121 vector is used for transformation, and the efficiency is remarkably improved compared with that of an existing method. The method provides an efficient and reliable technical platform for root system gene function research, expression analysis and genetic improvement of salix cheilophila and related species thereof, and has high application potential.

The invention discloses a method for high-yield production of rare saponin Rg3 through gene editing of a panax notoginseng adventitious root saponin synthesis pathway, which comprises the following steps: 1) inputting a gene CYP716A53V2 into an online tool CRISPR to find a series of potential Cas9 target sites, and carrying out random selection; (2) respectively designing downstream primers according to different selected Cas9 target sites, and carrying out PCR (Polymerase Chain Reaction) amplification by using a U6p.4-F primer as an upstream primer to respectively obtain different sgRNA sequences; (3) respectively connecting the sgRNA-n with a linearized pP1C.4 carrier, so as to obtain a recombinant plasmid; 4) respectively transforming the recombinant plasmids into agrobacterium rhizogenes, and culturing to obtain different recombinant bacterial liquids; and (5) respectively putting the pseudo-ginseng adventitious roots into different positive recombinant bacteria solutions, carrying out vacuum infection, and screening out the transgenic pseudo-ginseng adventitious roots with high Rg3 content. The method has the advantages of controllable steps, accurate regulation and control, greenness, environmental protection and high yield of saponin Rg3.

The invention relates to the technical field of genetic engineering, in particular to an agrobacterium tumefaciens-mediated radix pseudostellariae hairy root genetic transformation method and application thereof. The method comprises the following steps: preparing a radix pseudostellariae explant; activating and culturing agrobacterium rhizogenes; constructing an engineering bacterium containing a target gene recombinant vector; infecting explants and inducing hairy roots; carrying out resistance screening and sterilization on transgenic hairy roots; carrying out amplification culture on hairy roots; and carrying out RT-PCR detection. The hairy root genetic transformation method provided by the invention is simple and rapid to operate and high in gene expression level, and can provide technical support for gene function research. Meanwhile, by using the genetic transformation method provided by the invention, through overexpression of the radix pseudostellariae cyclic peptide coding gene, a transgenic material with significantly improved content of the pharmacodynamic substance cyclic peptide of the radix pseudostellariae can be obtained, and a research material is provided for researching a biosynthetic pathway of the cyclic peptide.

The invention discloses a rubus cochinchinensis efficient genetic transformation method based on RUBY reporter gene and agrobacterium rhizogenes mediation, which realizes that the transformation period is shortened to be within 15 days and the transformation efficiency is improved to be more than 60% through direct explant treatment under a non-tissue culture condition. The method comprises the following specific steps: 1) preparing an explant material; (2) constructing an RUBY carrier and transforming agrobacterium rhizogenes; (3) infecting the explant by utilizing agrobacterium rhizogenes carrying a target gene; (4) co-culturing after infection and carrying out RUBY visual screening; 5) positive root molecular biology verification; (6) inducing positive roots into buds; according to the method, induction of the positive callus, the positive root and the positive bud of the rubus coreanus stem segment under the non-tissue culture condition is successfully achieved, a stable and efficient genetic transformation system is constructed, the method has the remarkable advantages of being easy and convenient to operate, low in cost, wide in applicability, high in reproducibility and the like, and a key technical support is provided for molecular breeding of the rubus coreanus.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention provides a method for carrying out genetic transformation on safflower carthamus in a tissue-free culture mode, which comprises the following steps: cutting off radicles of newly germinated safflower carthamus seedlings, taking the radicles as explants, infecting the explants in a mode of smearing agrobacterium rhizogenes containing a target gene on wounds, burying the infected explants in soil for culturing, and carrying out tissue-free culture on the explants to obtain the safflower carthamus. The transgenic safflower hairy roots are obtained. The method fills the blank of a safflower non-tissue culture genetic transformation system, the whole transformation period is 20-30 days, and the method can be applied to rapid and efficient genetic transformation, gene editing and variety improvement of safflower.

The invention relates to the technical field of agricultural product production, in particular to a pepper cuttage method and application. The invention provides a pepper cuttage method which comprises the following steps: taking 8-leaf-age lateral branches of pepper as cutting slips, punching the base parts of the cutting slips, then infecting the base parts of the cutting slips by using agrobacterium rhizogenes, and culturing for 36-60 hours under a dark condition. According to the method, agrobacterium rhizogenes induction and punching technologies are combined, the rooting speed of pepper cuttage can be greatly increased, the cuttage survival rate is increased, the seedling raising time is remarkably shortened, and the purpose of rapid and effective seedling raising is achieved. According to the pepper cutting method, limitation of traditional cutting seedling raising is broken through, additional nutrient solution does not need to be added, pepper root systems can rapidly grow in vermiculite, the cutting seedling raising time is shortened, and the effect of rapid seedling emergence and transplanting is achieved.

The invention belongs to the technical field of genetic transformation, and particularly relates to an agrobacterium rhizogenes-mediated genetic transformation method for catalpa bungei. The genetic transformation method of catalpa bungei comprises the following steps: obtaining stem segments of catalpa bungei after co-culture; after co-culture is finished, the stem segments of the catalpa bungei after co-culture are soaked with an antibiotic solution and then inserted into a degerming culture medium until transgenic hairy roots or positive roots grow out, and catalpa bungei plants with the transgenic hairy roots or positive roots grow out are obtained; transplanting the catalpa bungei plants with the transgenic hairy roots or positive roots, taking down the positive roots of the catalpa bungei plants after the catalpa bungei plants grow up, and performing root segment cuttage to obtain the whole transgenic catalpa bungei plants. The catalpa bungei genetic transformation method provided by the invention has relatively high infection efficiency on different genotypes of catalpa bungei, and the positive rate of genetic transformation is relatively high; and the catalpa bungei genetic transformation method can be suitable for different genotypes of catalpa bungei, and has genotype universality.

The invention belongs to the technical field of genetic engineering, and particularly relates to an agrobacterium rhizogenes-mediated coastal chrysanthemum indicum tissue culture-free genetic transformation method and application thereof in enhancing the salt tolerance of a root system. The agrobacterium rhizogenes-mediated coastal chrysanthemum indicum tissue culture-free genetic transformation method comprises the following steps: transfecting agrobacterium rhizogenes with plasmids containing to-be-transformed gene expression cassettes to obtain recombinant agrobacterium rhizogenes, transfecting stems with terminal buds of coastal chrysanthemum indicum with the recombinant agrobacterium rhizogenes, and then cutting. The method provided by the invention is short in required period, and the transgenic plant can be obtained only in 30-60 days. The problems of long chrysanthemum conversion rate period, complex operation and tedious process are solved. A tissue culture mode is not needed, so that the tedious process of tissue culture is reduced; local materials can be used according to the characteristic of cutting rooting of chrysanthemum, chrysanthemum seedlings do not need to be cultivated from seeds, and time cost is reduced.

The invention discloses application of an MrDRRP gene in regulating and controlling drought resistance of hairy roots of medicago ruthenica, and belongs to the technical field of biology. According to the invention, a disease-resistant related gene, named as MrDRRP, derived from medicago ruthenica is utilized, the medicago ruthenica is transformed through an agrobacterium rhizogenes mediated transgenic technology, the MrDRRP transgenic hairy roots of the medicago ruthenica are obtained, and drought stress experiments prove that the drought tolerance of the obtained hairy roots of the medicago ruthenica is remarkably enhanced, and the length and fresh weight of the hairy roots are remarkably improved. Therefore, the method is provided for cultivating the drought-resistant medicago ruthenica, the drought resistance of the medicago ruthenica is enhanced, the planting range of the medicago ruthenica is widened, and the method has good application value.

This invention discloses a banana-specific alkaline biocontrol organic fertilizer and its preparation method, belonging to the field of bio-organic fertilizer technology. The banana-specific alkaline biocontrol organic fertilizer of this invention comprises the following raw materials: 25-30 parts modified tobacco straw carbonized powder, 20-25 parts moringa seed fermentation product, 5-10 parts cactus fermentation product, 10-14 parts seaweed residue, 8-12 parts humic acid powder, and 3-5 parts shell powder. The moringa seeds are fermented using enzymatic hydrolysis and microbial agents, effectively breaking down large molecules into smaller molecules that can be directly absorbed by banana plants, promoting plant growth. Simultaneously, *Achromobacterium solani* and *Acinetobacter rhizogenes* secrete various active substances, significantly enhancing banana disease resistance and growth promotion. This invention's organic fertilizer, with its synergistic effect of multiple substances, reduces the use of chemical fungicides, effectively inhibits banana wilt disease, neutralizes soil acidity, increases banana yield, and improves economic and social benefits.

The application discloses a soybean alkali-resistant gene GmFPS1 and application thereof. The soybean farnesyl pyrophosphate synthase gene GmFPS1, and a nucleotide sequence of the GmFPS1 gene is shown as SEQ ID NO. 1. The application also discloses application of the soybean farnesyl pyrophosphate synthase gene GmFPS1 in construction of alkali-resistant transgenic crops. The application carries out genetic transformation of a plant alkali-resistance related protein coding gene GmFPS1 into transgenic Arabidopsis and Agrobacterium rhizogenes-mediated soybean chimera, and studies alkali stress resistance of the transgenic Arabidopsis and Agrobacterium rhizogenes-mediated soybean chimera. The transgenic Arabidopsis and Agrobacterium rhizogenes-mediated soybean chimera obtained have improved alkali stress resistance. The result of qRT-PCR also shows that the soybean GmFPS1 is induced in response to alkali stress, and the expression level is significantly up-regulated. Therefore, the plant alkali-resistance related protein coding gene GmFPS1 is introduced into crops by a genetic engineering method, and a new alkali stress-resistant transgenic plant variety can be obtained.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

The invention discloses a method for efficiently inducing adventitious buds and rooting through transgenic hairy roots of Populus albus Nanlin 895, and relates to the technical field of biological breeding. The plant material used in the invention is a transgenic Nanlin 895 poplar hairy root generated by mediation of agrobacterium rhizogenes; dividing the hairy roots into root sections of 1-2 cm by using a sterile blade, and putting the hairy roots on a culture medium added with 0.01 mg / L TDZ and 0.2 mg / L 6-BA to induce adventitious buds; placing the adventitious buds in a culture medium added with 0.01 mg / L of TDZ for bud elongation; and cutting off the elongated adventitious buds, placing the adventitious buds in a culture medium added with 0.1 mg / L IBA, and carrying out rooting culture so as to obtain a positive Nanlin 895 poplar transgenic plant. Experiments prove that the adventitious bud induction rate of the plant material is 70%, the adventitious bud elongation rate is 40%, the rooting rate is 90%, and the method has good practicability.

The application provides application of a GmERF5 gene in drought resistance and salt stress resistance, and belongs to the technical field of genetic engineering.The GmERF5 gene is cloned into a pCAMBIA3301 vector to obtain a recombinant vector; the obtained recombinant vector is transformed into Agrobacterium rhizogenes, and a transgenic complex plant is obtained by using the obtained Agrobacterium rhizogenes to infect seeds, so that overexpression of the GmERF5 gene is realized.The overexpression of the GmERF5 gene can improve the antioxidant enzyme activity of the transgenic soybean plant, and the drought and salt stress resistance of the soybean is enhanced.The soybean GmERF5 of the application provides a new regulation gene resource for improving the drought resistance and salt tolerance of soybean, can be used for cultivation and improvement of stress-resistant soybean materials, lays a theoretical foundation for drought resistance and salt molecular mechanism, and simultaneously provides a theoretical basis and gene resource for soybean drought resistance and salt molecular breeding.

The invention provides a flora and an application of the flora in biodegradation of polyurethane plastics. The flora is prepared from raoultella ornithinolytica, klebsiella pneumoniae, coxakella rhizogenes and achromobacter xylosoxidans. According to the method, the polyurethane degrading flora is screened from the collected soil sample, the flora can efficiently degrade waterborne polyurethane and thermoplastic polyurethane elastomers with large chemical structure difference at the same time to degrade polyurethane plastic, the process is greatly simplified, the operation cost is reduced, traditional landfill incineration is replaced, green circulation of polyurethane is assisted, and the method has the advantages of being environmentally friendly and environmentally friendly. The method has important practical significance for solving the problem of environmental pollution caused by polyurethane waste and promoting green cyclic utilization of polyurethane materials.

The present invention discloses a method for inducing transgenic hairy roots in the stems of Carya serrata, belonging to the field of forest genetic engineering. The method utilizes Agrobacterium rhizogenes to infect semi-lignified or lignified stems of Carya serrata to obtain stable transgenic hairy roots. These roots can be induced to develop within one to two months, with a rooting rate as high as 94.43% and a transgene-positive rate of 98.38%. This method provides a cost-effective, tissue culture-independent transgenic method, and clearly demonstrates that transgenic roots can be used to verify gene function. This method overcomes the technical barriers to stable genetic transformation in Carya serrata, providing technical support for the research of functional genes.

The invention belongs to the technical field of plant genetic engineering and biotechnology, and discloses a construction method of a Hainan camellia oleifera seedling transgenic hairy root system, which comprises the following steps: selecting a 3-month-old camellia oleifera seedling explant Haidao camellia oleifera No.4, pretreating, transferring a target plasmid into agrobacterium rhizogenes K599 by an agrobacterium-mediated method, and culturing to obtain the Hainan camellia oleifera seedling transgenic hairy root system. Preparing an infection solution, optimizing infection conditions, inducing hairy roots to generate, and finally verifying the transgenosis effect through fluorescence observation and molecular identification. According to the method, an infection liquid formula, infection conditions and culture conditions are optimized, the induction efficiency of the transgenic hairy roots is remarkably improved, technical support is provided for functional gene research and genetic improvement of Hainan camellia oleifera, and the method has important application value.

An Agrobacterium rhizogenes mediated method for high-efficiency genetic transformation and gene editing in Brassica crops. A genetic transformation and gene editing system for Brassica crops is established by means of Agrobacterium-rhizogenes-mediated delivery of three growth and development regulatory factors, i.e. ZmWUS2, AtIPT and AtPLT5. By means of the Agrobacterium-rhizogenes-mediated delivery of the three growth and development regulatory factors, i.e. ZmWUS2, AtIPT and AtPLT5, explants are induced to form calli, from which shoots are directly formed, so that the problem of difficult regeneration of roots of Brassica crops is solved, thereby achieving high-efficiency transformation in species with poor root regeneration capacity.

The invention discloses a Ningxia wolfberry genetic transformation method based on adventitious bud induction. The agrobacterium rhizogenes are adopted for genetic transformation, and the problem that buds of traditional Ningxia wolfberry leaves are difficult to regenerate is effectively solved. The agrobacterium rhizogenes can effectively induce formation of hairy roots of the Ningxia wolfberry, so that the technical bottleneck in the regeneration process of the Ningxia wolfberry is broken through, and the transformation efficiency is remarkably improved. In the transformation process, although the Ningxia wolfberry hairy roots induced by agrobacterium rhizogenes successfully obtain transgenic markers, the original adventitious bud regeneration capacity is inhibited. By means of the dedifferentiation and repeated induction culture technology, the redifferentiation capacity of the hairy root induced callus is effectively recovered, the regeneration bud forming efficiency of the hairy root induced callus is remarkably improved, and a transgenic Ningxia wolfberry plant is obtained.

The invention belongs to the technical field of genetic transformation, and particularly relates to a poplar genetic transformation method suitable for an outdoor environment. The method comprises the following steps: obtaining a poplar scion, cutting the poplar scion into 18cm cutting slips, soaking the cutting slips in an activated agrobacterium rhizogenes solution for 30 minutes to obtain an infected scion, performing soil culture or water culture, and transplanting the scion into a large container for continuous culture after transgenic positive roots grow out. The method provided by the invention does not need to depend on sterile conditions, genetic transformation can be realized through cuttage and water culture in an outdoor environment, and the method has the advantages of high throughput and large scale.

The invention discloses a method for promoting strawberry root system development by using K599 agrobacterium rhizogenes. The method comprises an assembly type elevated cultivation device, seedling cultivation management and a method for promoting root system development. And the overhead cultivation device is adopted, so that the problems of gray mold and the like caused by field cultivation can be reduced. Seedling cultivation management is used for helping propagation of strawberry resources, reserving variety characteristics and shortening the seedling period. And promoting root development to help strawberry seedlings to rapidly root, strengthen roots, strengthen seedlings in a short period of time and shorten the seedling raising period. Efficient cultivation management and a rooting promoting method are combined, and the propagation period of the strawberries is shortened. According to the method, the K599 agrobacterium rhizogenes are used for promoting rooting of strawberry seedlings and absorption of plant nutrient substances by root systems, the root length, the root tip number and the root specific surface area are remarkably increased by 19.85%, 39.63% and 68.79% respectively, and the root systems are developed and robust. The strawberry seedling strengthening time can be shortened, cost is low, operation is easy and convenient, and the effect is remarkable.

The present invention discloses a method for obtaining transgenic plants of Castanopsis chinensis. The base of the stem of Castanopsis chinensis seedlings is infected with Agrobacterium rhizogenes carrying a fluorescent reporter gene. After infection, the seedlings are cultured in pure vermiculite pots. Transgenic roots can be obtained in one month. After the transgenic roots are lignified, transgenic plants are obtained by root cutting. The method of the present invention is simple to operate, low in cost, high in efficiency, and short in cycle. The transgenic adventitious roots can be quickly verified using a handheld fluorescent protein excitation light source, and then transgenic plants are successfully obtained by transgenic root culture. The method of the present invention is an efficient method for obtaining stable transgenic plants by bypassing tissue culture. It can also be applied to the rapid verification of gene functions of Castanopsis chinensis and the genetic engineering breeding of Castanopsis chinensis, laying a good foundation for the future development of a gene editing breeding system for Castanopsis chinensis.

An induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof, a method for screening an inducing gene, and production and use, specifically belonging to the technical field of plant tissue culturing. The induction method for an Antirrhinum majus L. hairy root producing an anthocyanin and a glycoside derivative thereof comprises the following steps: using Agrobacterium rhizogenes containing an inducing gene to infect an Antirrhinum majus L. explant, co-culturing the Agrobacterium rhizogenes and the Antirrhinum majus L. explant, and performing sterilization culture to obtain the Antirrhinum majus L. hairy root producing the anthocyanin and the glycoside derivative thereof. The inducing gene comprises a gene encoding an MYB transcription factor, or a mutant thereof. The induction method can quickly and effectively obtain the hairy root synthesizing the anthocyanin and the glycoside derivative thereof while realizing the acquisition of the anthocyanin and the glycoside derivative thereof in a short time without being affected by seasons in the case of limited germplasm resources.

The invention provides a method for verifying gene functions based on combination of agrobacterium rhizogenes and a movable element. The provided method comprises the following steps: (1) constructing an expression vector fused by a target gene and a movable element, wherein the target gene is a functional gene to be verified of the plant; (2) transferring the expression vector into agrobacterium rhizogenes to obtain transformed agrobacterium rhizogenes; (3) infecting a plant by utilizing the transformed agrobacterium rhizogenes to obtain a positive plant with hairy roots; (4) determining the function of the target gene in the plant through the expression of the target gene in the upper end tissue of the positive plant and the phenotypic difference between the target gene and the control plant; wherein the movable element is a genetic material capable of transferring the target gene from the hairy root of the positive plant to the upper tissue. The method provided by the invention is simple to operate, high in transformation efficiency and stable and reliable in phenotype, and can be widely applied to plant gene function research.

The application provides a method for inducing transgenic hairy root formation of Lagenaria vulgaris by non-tissue culture, comprising the following steps: (1) Lagenaria vulgaris culture: sowing Lagenaria vulgaris seeds in a seedling substrate, and culturing in a light incubator until the Lagenaria vulgaris plant grows to a stage between one heart and one leaf and one heart; (2) infection by a hole-punching and smearing method: taking the cotyledon node to 1 cm below the cotyledon node of the Lagenaria vulgaris plant as an infection site, punching a hole around the infection site, and smearing the hole with Agrobacterium rhizogenes; (3) nodule induction: continuing to culture the Lagenaria vulgaris plant in the light incubator, and observing that the infection site is swollen; and (4) inducing hairy root formation by cutting and culturing: obliquely cutting the swollen position of the infection site of the Lagenaria vulgaris plant, and inserting the lower end of the obtained upper plant, i.e. the obliquely cut end, into a growth carrier for culture to induce hairy root formation. The application can induce transgenic hairy root formation of Lagenaria vulgaris, has a high induction rate, and can be applied to promoter function research, functional gene analysis, secondary metabolite research and the like.