Agrobacterium rhizogenes transformation method for living body growth cone of woody plant

A technology for transformation of Agrobacterium and woody plants, which is applied in the field of Agrobacterium transformation method of living growing cones, can solve the problems of difficulty in reproducing, low transformation frequency, unstable effect, etc., achieves high relative success rate, high transformation efficiency, avoids gene ethereal effect

Inactive Publication Date: 2011-01-05
CHONGQING ACAD OF AGRI SCI
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, woody plants generally contain a large amount of polyphenols. Polyphenols accumulate rapidly and massively in wound cells, which is a self-protection mechanism for plants. Gene transformation has an inhibitory effect, and even has a killing effect on Agr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Agrobacterium rhizogenes transformation method for living body growth cone of woody plant
  • Agrobacterium rhizogenes transformation method for living body growth cone of woody plant
  • Agrobacterium rhizogenes transformation method for living body growth cone of woody plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. In view of the fact that tea tree is a perennial woody plant with high polyphenol content, it is a typical representative of woody plants. Therefore, in a preferred embodiment of the present invention, tea trees are used as the research object, and the reporter gene gus is transformed into 5 genotypes of tea trees with a total of about 100,000 growth cones by the Agrobacterium transformation method of living growth cones, and finally identified by kanamycin resistance, GUS staining identification and PCR identification, a total of 32 transgenic plants were obtained. The Agrobacterium transformation method of living growth cones of other woody plants can refer to the transformation method of Agrobacterium growth growth of tea trees in vivo. The basic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an agrobacterium rhizogenes transformation method for a living body growth cone of a woody plant, which comprises multiple steps of preparing agrobacterium rhizogenes engineering bacterium osmosis transformation liquid, converting and inoculating the living body growth cone, coculturing the growth cone and agrobacterium rhizogenes engineering bacteria, screening resistant sprouts, culturing resistant branches, identifying a transgenic plant, and the like. The invention breaks through two technical bottlenecks of difficult gene transformation and difficult regeneration of the woody plant, is independent of plant genotype and tissue culture, has the advantages of high transformation efficiency, simple operation, low cost, and the like and is suitable for most of woody plants, such as tea trees, and the like, especially the woody plant with full terminal buds.

Description

technical field [0001] The invention relates to a plant gene transformation method, in particular to a method for transforming agrobacteria in living bodies of woody plants such as tea trees. Background technique [0002] Plant genetic transformation refers to the introduction of exogenous genes into the recipient plant genome through a certain technology or approach, so that it can be stably inherited in the recipient plant, and endow the plant with new agronomic traits, such as high resistance, high yield, high quality, metabolic Significant changes in substance content, etc. [0003] In plant genetic transformation, there are usually two types of gene receptors: in vitro culture materials and living materials. Using in vitro culture materials such as protoplasts, suspension culture cells, callus, microspores and original explants as gene recipients, that is, to achieve genetic transformation through tissue culture and plant regeneration, is currently the most important m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/82A01H5/00
Inventor 姚永宏柴友荣李中林周正科吴全
Owner CHONGQING ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap