Production method for high-density pure arbuscular mycorrhizal fungal spore

Abstract

The invention discloses a production method for high-density pure arbuscular mycorrhizal fungal spore, which belongs to the technical field of microorganism culturing, and comprises the following technical steps of: 1) culturing carrot root tissues converted from agrobacterium rhizogenes plasmid DNA; 2) culturing the carrot root tissues converted from agrobacterium rhizogenes plasmid DNA and glomus intraradices together in an improved synthetic culture medium; 3) taking a culture containing the root, hypha and spore infected by the arbuscular mycorrhizal fungus and transplanting the culture to a special culture box for culturing; 4) collecting the spore and mycelium; and 5) storing the arbuscular mycorrhizal fungal spore. Compared with the prior art, the production method for high-density pure arbuscular mycorrhizal fungal spore has the advantages that: 1, the culture medium is an asepsis environment with low cost, which not only can meet the growing of a host plant, but also is suitable for the growing of AM epiphyte; 2, the nutrition requirements for the growing of the host plant are low and the host plant can grow and be cultured on the synthetic culture medium; 3, the spore cultured by the method is a non-pollution pure culture; and 4, the produced spore is stored in liquids and the activity can be maintained by above 90 percent.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a high-density pure production method of arbuscular mycorrhizal fungal spores. Background technique [0002] Arbuscular Mycorrhizae (AM) is a fungus that is ubiquitous in nature and symbiotic with more than 80% of land plants. In nature, AM fungi have a wide range of hosts and distributions, and can form a symbiotic relationship with most plants. So far, more than 150 kinds of AM fungi have been found to form symbiosis with plants, which is one of the most common types of mycorrhizae. . It has a mutualistic symbiosis with the host plant: the extra-root mycelium of the fungus absorbs phosphorus and other nutrient elements from the soil to supply the host plant, improves the plant's ability to absorb mineral elements, promotes plant growth, etc., and the host plant supplies what is necessary for the growth of the fungus of carbohydrates. Due to the impo...

AI Technical Summary

Problems solved by technology

The advantage of this method is that a very pure bacterial agent can be obtained, but the cost is high and the yield is low

[0005] Commercial production of arbuscular mycorrhizal fungi is carried out by co-cultivating host plants and arbuscular mycorrhizal fungi for 4-5 months under greenhouse conditions. Since arbuscular mycorrhizal fungi cannot be cultured alone without host plants, Therefore, it is very difficult to produce the bacterial agent on a large scale, and the type of host plant, the formula of the culture substrate, and the culture conditions are all the keys to the production process.