80 results about "Arbuscular mycorrhizal" patented technology

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

The invention relates to a compound soil remediation agent containing microorganism bacteria and a preparation method thereof. The remediation agent comprises the following components in parts by weight: 0.01-3 parts of bacillus pasteurii spore powder, 5-50 parts of phosphogypsum powder, 10-60 parts of organic compost, 5-50 parts of turf powder, 1.5-5 parts of potassium fulvate powder, 0.2-0.5 part of powdery bacteria powder carriers and 0.15-1 part of glycerin. The remediation agent has round and smooth grains and high grain hardness, is harmless to seeds, can serve as a base fertilizer together with other fertilizers, can be sown together with seeds, integrates multiple remediation functions including organic complexation and passivation remediation, inorganic passivation remediation, microorganism passivation remediation and the like, has a comprehensive and strong soil remediation function, and can stimulate growth and reproduction of arbuscular mycorrhizal fungi at the roots of crops, accelerate heavy metal passivation, stimulate crop growth and enhance the immunities of crops.

The invention discloses a method for improving the phytoremediation efficiency of low-concentration arsenic-contaminated soil, which comprises the steps of: planting corns in an arsenic-contaminated farmland, inoculating arbuscular mycorrhizal fungi and earthworms, pulling up the corns with roots after the growth season of the corn is over, harvesting the corns wholly, and performing centralized treatment. The method fully exploits the potentials of arsenic remediation of plants, microorganisms and animals, and inoculates the AM fungi and the earthworms to promote the plant absorption and dissipation of arsenic by promoting the growth of the plants, improving the activity of phosphatase of the soil and activating the arsenic in the soil to further achieve the aim of improving the phytoremediation efficiency of the low-concentration arsenic-contaminated soil. The method combines the microorganisms, the animals and the plants together for the in-situ remediation of the arsenic-contaminated farmland, has simple and convenient operation and friendly operating environment, cannot cause secondary pollution, reduces the treatment cost, and has good environmental effect.

The invention relates to a method for promoting growth of sweet sorghum in a saline-alkali land with arbuscular mycorrhizal fungi and an organic fertilizer, sweet sorghum seeds are sown in the saline-alkali land, the arbuscular mycorrhizal fungi are inoculated and the organic fertilizer is applied simultaneously, and conventional field management is performed in the growing process of the sweet sorghum until harvesting. According to the method, the buffering performance and adsorption capacity of the soil can be enhanced by the aid of the actions of adsorption, complexing, reduction, volatilizing and the like of the organic fertilizer, the toxic action of saline ions is reduced, and the plant growth is promoted; the effectiveness of the saline ions is reduced by the aid of the chelation, the hypha immobilization function and the like of the arbuscular mycorrhizal fungi, the plant tolerance is improved in various ways of improving the plant nutriture, changing the rhizosphere physical and chemical properties, changing the root morphology, adjusting the expression of some salt-resistant genes and the synthesis of related proteins and the like, and the arbuscular mycorrhizal fungi and the organic fertilizer are applied simultaneously, so that saline-alkali stress can be relieved synergistically.

The invention provides an arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil and relates to a microorganism, in particular to an arbuscular mycorrhizal fungus, solving the problem that the effect of the existing method adopting organisms to repair the atrazine-contaminated soil is unsatisfactory. Moses sacculus mildew HDSF1 is preserved in the Common Microorganism Center of China Committee for Culture Collection of Microorganisms, with the preservation number of CGMCC No. 3012. After the Moses sacculus mildew HDSF1 is inoculated in the soil contaminated by atrazine, the growing trend of a crop plant sown on the soil is good, and the biomass is obviously higher than that not inoculated with the repairing arbuscular mycorrhizal fungus of the invention; and the residual quantity of the atrazine in the soil is greatly reduced. In the arbuscular mycorrhizal fungus for repairing the atrazine-contaminated soil, the Moses sacculus mildew HDSF1 is not influenced by indigenous microorganism contamination in the repaired soil, and the anti-interference property is strong; and under the condition that the concentration of the atrazine in the soil is lower, favorable biological degradability can still be kept, and the biological activity is kept.

The invention relates to a biological type organic-inorganic compound fertilizer. The biological type organic-inorganic compound fertilizer comprises, by weight, 30-37 parts of organic fertilizers, 10-15 parts of urea, 20-25 parts of ammonium chloride, 5-5.5 parts of ammonium sulfate, 10-20 parts of monoammonium phosphate, 10-12 parts of calcium superphosphate, 10-15 parts of potassium chloride and 0.1-0.12 part of arbuscular mycorrhizal fungal inoculants. According to the technical scheme, the biological type organic-inorganic compound fertilizer has the advantages that the biological type organic-inorganic compound fertilizer manufactured by the aid of the arbuscular mycorrhizal fungal inoculants is high in utilization rate, convenient to use, wide in practicality and widely applicable to different types of soil and is environmental friendly.

The invention discloses a soil microorganism improvement method for enhancing water holding and fertilizer increasing. Decomposed coal is applied into loess or sandy soil, and arbuscular mycorrhizal fungi are added to be fully mixed. Formed improved soil comprises the loess or the sandy soil, the decomposed coal applied into the loess or the sandy soil and the arbuscular mycorrhizal fungi. The optimal proportion of the applied decomposed coal is that the mass ratio of the decomposed coal and the loess or the sandy soil is between 1:2 and 1:1. According to the soil microorganism improvement method for enhancing water holding and fertilizer increasing, the decomposed coal is applied into the soil and is mixed with the loess or the sandy soil to form different ratios, then the decomposed coal and the loess or the sandy soil is inoculated with the arbuscular mycorrhizal fungi, and an obvious promoting effect is achieved on crop growth and water utilization. Accordingly, decomposed coal resources are efficiently utilized, the soil is improved, and fertilizer increasing and water holding are achieved.

The invention discloses a convenient and simple expanding propagation method of arbuscular mycorrhizal fungus inoculant and an structure thereof. The expanding propagation method comprises the steps of a, refining seedling soil in a greenhouse through a 1mm mesh, and conducting steam sterilizing on the seedling soil at 121 DEG C for 2 hours to obtain a sterilization substrate; b, sending clover seeds into 0.1% sodium hypochlorite solution to sterilize for 2 min, and then using distilled water to wash the clover seeds to obtain the sterilized seeds; c, using a pasteurization solution to conduct sterilization on an expanding propagation greenhouse and a seedbed; putting the sterilization substrate doped with the arbuscular mycorrhizal fungus inoculant into the seedbed of the greenhouse, and adopting a broadcast sowing mode to sow the sterilized seeds; controlling temperature and humidity in the greenhouse at regular temperature, and cultivating for 3-4 months to obtain the arbuscular mycorrhizal fungus inoculant. According to the convenient and simple expanding propagation method of the arbuscular mycorrhizal fungus, expanding propagation replaces an existing pot expanding propagation with scalable greenhouse direct expanding propagation. The convenient and simple expanding propagation method of the arbuscular mycorrhizal fungus can increase the yield of the expanding propagation fungi, the substrate component is simple in structure, the materials are easy to obtain, and the expanding propagation method is low in cost and convenient for large-area expanding propagation.

The invention discloses a microbial multiple-effect organic fertilizer which is characterized by comprising the following components in parts by weight: 30-55 parts of chicken manure, 25-35 parts of rice bran, 34-46 parts of wheat bran, 10-24 parts of yam, 5-9 parts of sucrose, 9-15 parts of agar, 3-8 parts of ground phosphorite, 1-4 parts of ammonium dihydrogen phosphate, 1-3 parts of sodium acid pyrophosphate, 0.1-0.3 part of azotobacter powder, 0.1-0.3 part of phosphobacteria powder, 0.1-0.3 part of arbuscular mycorrhizal fungus powder, 15-35 parts of soybean straw and 10-25 parts of potato straw. The microbial multiple-effect organic fertilizer can effectively control underground pest damage, reduce the reproduction rate of pests and kill the underground pests, and has the effect of resisting drooping diseases, blight, rust, greensickness, root rot and other multiple diseases.

The invention relates to an application of arbuscular mycorrhizal fungi in reducing the content of cadmium in rice grains planted in a cadmium contaminated planting substrate, and a method capable of reducing the content of heavy metal cadmium in the rice grains. The method capable of reducing the content of the heavy metal cadmium in the rice grains provided by the invention comprises the following steps: (1) adding the arbuscular mycorrhizal fungi into the cadmium contaminated planting substrate, wherein the content of the cadmium in the cadmium contaminated planting substrate is 0.60 to 1.35 mg/kg-1; (2) directly sowing disinfected and germination-accelerated rice seeds into the arbuscular mycorrhizal fungi added planting substrate obtained in the step (1); and (3) culturing rice until the rice is mature and rice grains are produced. The method capable of reducing the content of the heavy metal cadmium in the rice grains provided by the invention adopts a plant symbiotic microorganism, i.e., the arbuscular mycorrhizal fungi, so the content of the heavy metal cadmium in the rice grains can be reduced; soil properties may not be affected; and soil may not be polluted.

The invention relates to a method for lowering cadmium content in soybeans by AM (arbuscular mycorrhizal) fungi. The method includes the steps such as soil selecting, field cultivating of the AM fungi, planting of the soybeans, field management, analysis on infection rate of the AM fungi, and measurement on the cadmium content. By the use of the method, accumulation of soybean biomass can be promoted, and Cd content is evidently reduced; secondary pollution caused by the use of the traditional Cd repairing method wherein various chemicals are added into the soil can be avoided; the cost is saved. Therefore, the method is of great significance and popularization value to the soybean food safety and the soybean planting management.

The invention discloses an acid paddy soil modifier. The composition of the modifier is prepared from biomass black charcoal, dolomite, sepiolite, limestone, zeolite, vermiculite, bentonite, coal ash,polyacrylamide, arbuscular mycorrhizal fungus inoculant, pig manure fermented substrates and super absorbent resin according to a certain proportion. By means of the acid paddy soil modifier, acid paddy soil can be modified, the soil pH value is increased, the soil structural stability is improved, dissolution of heavy metals in the soil is reduced, the contents of nutrient elements in the soil are improved, the modifier plays a part in promoting growth and yield improvement of paddies, a foundation is laid for widely modifying the south acid paddy soil, and thus the modifier has wide application prospects.

The invention provides a preparation method of a compound organic fertilizer for a saline and alkaline land, relates to the field of fertilizers, and solves the problems that the water retention capacity is poor, the action time is short, and and long-acting and long-lasting treatment cannot be realized in the conventional organic fertilizer for a saline and alkaline land. The organic fertilizer includes 20-25 parts of excrements of people and livestocks, 35-40 parts of plant straws, 6-8 parts of bentonite, 5-8 parts of potassium fulvic acid, 8-10 parts of humic acid, 7-10 parts of porous light-weight volcanic rock, 10-15 parts of pharmacy leftovers from a pharmaceutical factory, 9-12 parts of a compound water-retaining agent, 3-5 parts of a sandy soil adhesive, 1-2 parts of bacillus subtilis, 1-2 parts of arbuscular mycorrhizal, 1-2 parts of trichoderma harzianum, 1-2 parts of salt-tolerance rhodopseudomonas capsulata, 1-2 parts of jelly bacillus, 1-2 parts of phosphate solubilizing bacteria, 1-2 parts of potassium bacteria and 1-2 parts of saccharomycetes. According to the invention, the soil structure of the saline and alkaline land is effectively improved, the fertility is improved, the saline and alkaline is reduced, the soil water retention capacity is improved, accumulation of salt is prevented, and the action is long-lasting.

The invention discloses a remover for removing mercury and cadmium ions in saline-alkali soil and a preparation method thereof. The remover comprises the following raw materials in parts by weight: 30to 50 parts of ultrafine mineral-originated humus powder, 5 to 10 parts of white-rot fungus liquid, 6-10 parts of an arbuscular mycorrhizal fungus root system inoculant, 5-10 parts of citric acid, 2-5 parts of soil moisture preservation agent, 20-30 parts of glutinous rice wine residue powder, 1-5 parts of a soil moisture preserving agent. The ultrafine mineral-originated humus powder can loosensoil and improve air permeability and water permeability, and is rich in element amount, and a carbon source and a nitrogen source included in the ultrafine mineral-originated humus powder provide nutrients required by microbial growth; the soil moisture preservation agent has strong water retention and storage effects, and can reduce the evaporation speed of water in soil; the arbuscular mycorrhizal fungus root system inoculant can inhibit heavy metal ion absorption of crops; white-rot fungi can degrade heavy metal ions in soil; and citric acid can adsorb heavy metals in soil in a short time,and the acidity of the citric acid can reduce the pH value of the saline-alkali soil.

The invention discloses an excellent rice seedling growing matrix. The excellent rice seedling growing matrix is composed of vermiculite, slag, peat soil, reed residues, perlite, arbuscular mycorrhizal fungicide, sugarcane filter mud leavening, manioc waste leavening, coco coir, fine vinegar residue and diatomite according to a certain proportion. According to the excellent rice seedling growing matrix, by selecting and matching multiple organic and inorganic seedling growing matrixes, the volume weight, porosity, electric conductivity (EC), pH, main nutrients of nitrogen, phosphorus, potassium and organic matter and other physiochemical properties of the seedling growing matrix are significantly optimized, the quality of rice seedlings is significantly improved, and various indexes for seedling growing through mechanical transplanting are satisfied. The excellent rice seedling growing matrix also has the advantages that the physiochemical properties are stable, the price is low, materials are convenient to take, the pollution to the environment is small, the matrix is easy to treat after being used, the practicability is high, and the application prospect is wide.

The invention belongs to the technical field of fertilizer preparation, and discloses a biological fertilizer and a preparation method thereof. The biological fertilizer is prepared from the ingredients of a culture substrate and a carrier substrate, wherein the culture substrate is prepared from glucose, yeast extracts, beef extracts, peptone, primary fermentation bacteria, secondary fermentationbacteria, and tertiary fermentation bacteria; the carrier substrate is prepared from bean cake, rapeseed cake and wheat bran according to a mass ratio of 1 to 2 to 3. Meanwhile, a preparation methodis disclosed. Arbuscular mycorrhizal fungi capable of forming a symbiotic system with most crops for promoting the plant growth is used; the rhizobium fertilizer can achieve a stable effect since therhizobium fertilizer can form the symbiotic system with the plants; the arbuscular mycorrhizal fungi belongs to another kind of microbes capable of forming a symbiotic system with various kinds of plants; the plant growth can be promoted; the crop yield can be improved; a stable effect is achieved.

The invention discloses a biofertilizer for green food tea. The biofertilizer is characterized by containing the following substances in parts by weight: 35-40 parts of urea, 11-16 parts of ammonium chloride, 15-26 parts of diammonium phosphate, 25-30 parts of weathered coal, 15-30 parts of bamboo charcoal powder, 0.0001 part of photosynthetic bacterium powder, 0.0001 part of arbuscular mycorrhizal fungus powder, 0.0001 part of bacillus licheniformis powder, 25-30 parts of duck manure, 15-20 parts of cotton stalk powder, 25-30 parts of oilseed straw powder, 15-20 parts of goose feces, 15-20 parts of sarcandra glabra, 3-6 parts of ash barks, 3-5 parts of radix stemonae, 11-20 parts of potassium sulfate, 10-20 parts of sodium chloride, 1-2 parts of calcium superphosphate and 3-6 parts of ammonium molybdate. The biofertilizer has the beneficial effects that the fertilizer is scientific in formula, contains microelements and natural sustained-release substances and can meet the growth needs of the tea, so that the yield of the tea can be increased, the quality of the tea can be improved, meanwhile, the soil can be improved, and the ecological balance of the soil is maintained.

The invention provides an application and method for increasing the cotton yield through AM fungus infestation of cotton and belongs to the field of AM fungus technology. The method comprises the steps that cotton seeds are placed into a cultivation box containing river sand for seedling culture; after the cotton seeds germinate and grow four leaves, arbuscular mycorrhizal fungus liquid inoculants are added to the roots of all cotton plants, and a nitrogen-limited phosphorus-limited nutrient solution is applied once every seven days during cultivation; the cotton plants are transplanted into cultivation pots containing sand; the nitrogen-limited phosphorus-limited nutrient solution is applied once every three days after the cotton plants are cultivated for ten days; after seedling cultivation is conducted for 40 days, the cotton plants are transplanted to a test field; after the cotton plants are transplanted to the test field for 30 days, compound fertilizer is applied to the roots of the cotton plants, and the cotton plants are watered every 15 days; after three months, the cotton is harvested. According to the method, the phosphorus content of the applied nutrient solution is very low, and the nitrogen content of the applied nutrient solution is high; in agricultural production application, application of phosphate fertilizer can be reduced, and accordingly the infestation rate of AM fungi and the cotton and the cotton yield are increased.

The invention discloses a special compound fertilizer for tea and a preparation method of the special compound fertilizer. The special compound fertilizer is prepared from the following raw materials in parts by weight: 26 parts of dried-up pond soil particles, 12 parts of corn umbilicus cakes, 6 parts of chick bone powder, 8 parts of shrimp shell meal, 10 parts of sweet potato starch, 10 parts of urea, 10 parts of brown sugar, 8 parts of iron oxide, 5 parts of phosphoric bacteria, 10 parts of monopotassium phosphate, 30 parts of chicken manure, 40 parts of human excrement, 50 parts of peat soil, 20 parts of coal ashes, 5 parts of arbuscular mycorrhizal fungi and 3.5 parts of microelements. The organic fertilizer contains a lot of organic nutrients, so that soil improvement is facilitated; the quality and the yield of the tea are improved; and the special compound fertilizer is a long-acting efficient organic fertilizer.

The present invention is directed to Mycorrhizal seed and in-furrow compositions containing paraffinic oil. The present invention is further directed to methods of improving plant growth by applying Mycorrhizal seed treatment and in-furrow compositions containing a paraffinic oil to a plant, plant propagation material, or an area where a plant will grow.

The invention provides a collection method of arbuscular mycorrhizal fungi, and belongs to the technical field of microorganism collection, which aims at solving the problems of time waste, labor waste, larger space, complicated greenhouse conditions or operation steps, higher cost and the like in the prior art. The collection method comprises the following steps of (1) separating arbuscular mycorrhizal fungi spores from a potted propagation expanding inoculant by a wet sieve decantation method and a sugarcane centrifuging method, and disinfecting, so as to obtain a suspension liquid of the arbuscular mycorrhizal fungi spores; (2) using sodium alginate, gelatin, carrageenan and polyvinyl alcohol as carriers, and embedding the suspension liquid of the arbuscular mycorrhizal fungi spores into the carriers, so as to obtain an inoculant mixture; (3) dripping the inoculant mixture into a sterile crosslinking agent in a physical extrusion way, preparing into ball-like matter, curing, and cleaning, so as to obtain embedding small beads of the arbuscular mycorrhizal fungi; (4) drying the embedding small beads, loading into a centrifugal tube, and refrigerating in a refrigerator at the temperature of 4 DEG C. The collection method has the advantages that the operation is simple, the cost is low, the occupation of a large amount of space is not needed, and the material and labor are reduced.

The invention discloses a compound organic substance fertilizer for tea trees, which is characterized by comprising the following substances in parts by weight: 20-30 parts of biogas residue, 25-30 parts of weathered coal, 15-30 parts of bamboo charcoal powder, 0.0001 part of photosynthetic bacterium powder, 0.0001 part of arbuscular mycorrhizal fungus powder, 0.0001 part of Bacillus licheniformis powder, 25-30 parts of duck manure, 15-20 parts of cotton straw powder, 25-30 parts of oil seed straw powder, 15-20 parts of geese manure, 15-20 parts of sarcandra glabra, 3-6 parts of Cortex Fraxini, 3-5 parts of stemona, 11-20 parts of potassium sulfate, 10-20 parts of wheat straw powder, 12-15 parts of peanut straw powder, 15-19 parts of mung bean straw powder, 1-3 parts of humic acid, 0.1-0.3 part of amino acid, 0.3-0.6 part of chitosan, 15-20 parts of peanut cake, 4-8 parts of sodium aluminum phosphate and 2-5 parts of meerschaum. The fertilizer disclosed by the invention is reasonable in composition, balanced in nutrition, safe and pollution-free, completely satisfies long-term and short-term nutrient demands of tea trees, can balance fertilizer application, soil improvement and environment protection, and can effectively increase the output and quality of tea.