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Application and method for increasing cotton yield through AM fungus infestation of cotton

A cotton and fungus technology, applied in the application field of AM fungus infecting cotton to increase cotton yield, can solve the problems of less research on the application of Glomus intraroote, achieve ecological environment friendliness, reduce the use of phosphate fertilizer, and increase the infection rate Effect

Inactive Publication Date: 2015-05-13
ZHEJIANG NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of arbuscular mycorrhizal fungi mainly focuses on Glomus mosseae, Glomus fasiculatum and Glomus fasiculatum Glomus intraradices (Gigaspora margarita), while there is less application research on Glomus intraradices (Glomus intraradices), Glomus intraradices can be said to be arbuscular mycorrhizal a class of model fungi

Method used

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  • Application and method for increasing cotton yield through AM fungus infestation of cotton
  • Application and method for increasing cotton yield through AM fungus infestation of cotton
  • Application and method for increasing cotton yield through AM fungus infestation of cotton

Examples

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Embodiment

[0027] 1) Soak cotton seeds in tap water for 1 day, then put the soaked cotton seeds into a culture box containing river sand to grow seedlings, put the culture box in a light incubator for cultivation, and the cultivation conditions are day and night temperature 18 / 28°C , the humidity is 70%, the light intensity is 100%, and cultivated for 1-2 weeks;

[0028] 2) When the cotton seeds germinate and grow 4 leaves, add 3ml of arbuscular mycorrhizal fungus liquid inoculum to the root of each cotton plant, the amount of spores in the inoculum is 200-300 / ml, and continue to cultivate for 4 weeks ;

[0029] 3) During the growth and cultivation process of step 2), add 5-10ml / plant of nitrogen-limited and phosphorus-limited nutrient solution every 7 days, and add an appropriate amount of water every day;

[0030] 4) After 4 weeks, the roots of the cotton were collected, and the infection rate of mycorrhizae was determined by the Trinyl blue staining-square cross method;

[0031] 5) ...

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Abstract

The invention provides an application and method for increasing the cotton yield through AM fungus infestation of cotton and belongs to the field of AM fungus technology. The method comprises the steps that cotton seeds are placed into a cultivation box containing river sand for seedling culture; after the cotton seeds germinate and grow four leaves, arbuscular mycorrhizal fungus liquid inoculants are added to the roots of all cotton plants, and a nitrogen-limited phosphorus-limited nutrient solution is applied once every seven days during cultivation; the cotton plants are transplanted into cultivation pots containing sand; the nitrogen-limited phosphorus-limited nutrient solution is applied once every three days after the cotton plants are cultivated for ten days; after seedling cultivation is conducted for 40 days, the cotton plants are transplanted to a test field; after the cotton plants are transplanted to the test field for 30 days, compound fertilizer is applied to the roots of the cotton plants, and the cotton plants are watered every 15 days; after three months, the cotton is harvested. According to the method, the phosphorus content of the applied nutrient solution is very low, and the nitrogen content of the applied nutrient solution is high; in agricultural production application, application of phosphate fertilizer can be reduced, and accordingly the infestation rate of AM fungi and the cotton and the cotton yield are increased.

Description

technical field [0001] The invention belongs to the technical field of AM fungi, and in particular relates to the application and method of AM fungus infecting cotton to improve cotton yield. Background technique [0002] Mycorrhiza is a symbiosis formed by plant roots and mycorrhizal fungi, and arbuscular mycorrhizal fungi are a type of mycorrhizal fungi that are ubiquitous in nature. 80% of terrestrial plants on the earth can form arbuscular mycorrhizal fungal symbiotic systems with them . A major point of reference for arbuscular mycorrhizal fungi to form symbiotic systems with plants is the point of invasion where AM fungi enter the root. At the same time, the number of invasion points per unit root length can be used as one of the parameters to measure the infection potential of AM fungi. That is, after an AM fungal agent inoculates plants, the more invasion points formed, the greater the infection potential of the agent. When the hyphae of AM fungi infect a large ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G1/00
CPCA01G7/06A01G22/00C05B7/00
Inventor 金海如周端頊
Owner ZHEJIANG NORMAL UNIVERSITY
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