Application and method for increasing cotton yield through AM fungus infestation of cotton
A cotton and fungus technology, applied in the application field of AM fungus infecting cotton to increase cotton yield, can solve the problems of less research on the application of Glomus intraroote, achieve ecological environment friendliness, reduce the use of phosphate fertilizer, and increase the infection rate Effect
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[0027] 1) Soak cotton seeds in tap water for 1 day, then put the soaked cotton seeds into a culture box containing river sand to grow seedlings, put the culture box in a light incubator for cultivation, and the cultivation conditions are day and night temperature 18 / 28°C , the humidity is 70%, the light intensity is 100%, and cultivated for 1-2 weeks;
[0028] 2) When the cotton seeds germinate and grow 4 leaves, add 3ml of arbuscular mycorrhizal fungus liquid inoculum to the root of each cotton plant, the amount of spores in the inoculum is 200-300 / ml, and continue to cultivate for 4 weeks ;
[0029] 3) During the growth and cultivation process of step 2), add 5-10ml / plant of nitrogen-limited and phosphorus-limited nutrient solution every 7 days, and add an appropriate amount of water every day;
[0030] 4) After 4 weeks, the roots of the cotton were collected, and the infection rate of mycorrhizae was determined by the Trinyl blue staining-square cross method;
[0031] 5) ...
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