694 results about "Biofertilizer" patented technology

The invention discloses a tea garden soil improvement method based on microbial communities. The tea garden soil improvement method aims at improving soil fertility and tea tree yield by means of precisely screening beneficial microorganisms, optimizing culture conditions and the like. Firstly, soil samples are collected from a tea garden area, gene high-throughput sequencing is carried out, soil microbial community composition information is obtained, and beneficial microorganisms are screened out. Then, the breeding speed and biomass of the beneficial microorganisms are optimized by adjusting a culture medium formula. When the bio-fertilizer is prepared, besides concentrated bacterial liquid, an organic carrier, inorganic nutrient elements and humic acid serving as a stabilizer are also added, and a proper amount of trace elements and a plant growth regulator are added. In spring or autumn, according to the physicochemical property of the soil, the bio-fertilizer is uniformly spread and ploughed into the soil layer. After fertilization, soil physicochemical properties and tea tree growth conditions are regularly detected to evaluate the soil improvement effect and adjust the fertilization strategy according to the soil improvement effect. The method is beneficial for improving the tea garden soil health condition and improving the tea yield and quality.

The invention discloses application of sodium alginate-carboxymethyl chitosan composite hydrogel in preparation of a biological bacterial fertilizer, the sodium alginate-carboxymethyl chitosan composite hydrogel is used for preparation of the biological bacterial fertilizer, and is further used for preparation of the biological bacterial fertilizer with plant growth-promoting rhizobacteria as an effective component, and the sodium alginate-carboxymethyl chitosan composite hydrogel can be stored for a long time and has a good application prospect. The growth promoting effect is stably exerted. The plant growth-promoting bacteria are effectively wrapped and efficiently delivered through a nanometer means to enhance the growth-promoting effect on crops, the adaptability of the plant growth-promoting bacteria to the acid environment is effectively improved, and the advantage of the plant growth-promoting bacteria for promoting plant growth in acid soil is expanded. The biological bacterial fertilizer is suitable for neutral and acidic environments, and the effects of the biological bacterial fertilizer in the aspects of soil remediation and improvement, crop quality and efficiency improvement, crop stress resistance improvement and the like are fully exerted, so that the purposes of losing weight and improving efficiency are achieved.

The invention discloses a compound microbial fertilizer and application thereof in field planting. The survival rate of the paenibacillus mucilaginosus HX-02 disclosed by the invention can be up to 62.8% after 170d under the condition of 38% of nitrogen, phosphorus and potassium. The strain is organically combined with bacillus velezensis RKN1111 and a nitrogen-phosphorus-potassium inorganic fertilizer (N + P2O5 + K2O = 38%), the compound microbial fertilizer is developed, the viable count of effective bacteria is not less than 1.5 billion / g, and the viable count of paenibacillus mucilaginosus is not less than 0.5 billion / g. The compound microbial fertilizer taking the strain as a main functional bacterium is applied to field corn planting, can replace part of chemical fertilizers and increase soil fertility, can reduce the use amount of the chemical fertilizers by 15.56%, and achieves the effects of promoting the growth of field crops and increasing the yield by 10.2%. The paenibacillus mucilaginosus HX-02 is separated from soil, is harmless to human, livestock and environment, and meets the requirements of green plant protection and agricultural sustainable development.

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention discloses a strain of saline-alkali-resistant phosphorus-solubilizing compost bacillus subtilis C007, which can enhance the survival and colonization of beneficial microorganisms in saline-alkali soil farmland soil and plant root systems, also has the functions of solubilizing phosphorus and secreting auxin (IAA), and plays a role in promoting the growth of plants. The compound microbial fertilizer can improve the soil environment and the agricultural ecosystem on one hand, can promote the growth of plants, and can achieve the purposes of reducing the application of chemical fertilizers and improving the soil fertility of saline-alkali land farmlands to a certain extent.

The invention discloses a method for preparing a complex microbial inoculant for degrading enteromorpha polysaccharide and optimizing culture conditions of the complex microbial inoculant, the complex microbial inoculant for degrading enteromorpha polysaccharide is prepared through antagonism detection and strain combination screening, and the complex microbial inoculant for degrading enteromorpha polysaccharide is obtained through methods such as a single factor test, a pramipt-Berman test, a steepest climbing test and response surface analysis. According to the method, the enteromorpha polysaccharide degradation strain is used as a raw material to prepare the complex microbial inoculant, and compared with a single strain, the complex microbial inoculant can combine the functions of various strains in one preparation, so that the specificity of a degradation product can be improved; the complex microbial inoculant does not generate harmful by-products in the degradation process, the pollution of degradation products to the environment is reduced, theoretical support and practical basis are provided for enteromorpha degradation and research and development of biological fertilizers, and a new thought is provided for degradation and efficient utilization of enteromorpha resources.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

The invention provides a water and soil loss treatment and carbon sequestration and sink increase coupling regulation and control method for bamboo resource development, which comprises the following steps: integrating adaptive technologies of soil, plants and industries, verifying the influence of different soil types and plant types on water and soil loss and carbon sequestration through field investigation and experiments, and optimizing technology integration. Aiming at the eroded inferior land, a fertility improving technology is developed, and the soil fertility is recovered and the plant growth is promoted through biological fertilizer and organic matter modifier experiments, so that further land erosion is prevented. Thirdly, the effect of bamboo forest root systems on soil stabilization is discussed, and the optimal planting density and management measures are formulated. According to the method, the binary opposition of treatment and development is broken, the adaptability of soil and plants is combined, an efficient bamboo forest economic utilization technology is developed, the dual goals of water and soil conservation and resource utilization are achieved, and sustainable development of local economy is promoted.

The invention provides a special fertilizing device for bio-fertilizer, and belongs to the technical field of fertilizing equipment.The special fertilizing device for bio-fertilizer is provided with two parallel rotary tillage parts which can dig a fertilizing ditch along a set path, a fertilizer bin is used for pouring bacterial fertilizer into the dug fertilizing ditch, and a soil covering part backfills soil on the two sides of the fertilizing ditch to cover the bacterial fertilizer; the two rotary tillage components are coaxially arranged on a rotating shaft, the two rotary tillage components are respectively arranged in the two inclined slides, and the rotating shaft penetrates through the two slides along the width direction of the fertilization ditch; the soil covering part is located on the side, close to the edge of the fertilization ditch, of the second slide, the second slide can convey the soil turned and stirred by the second rotary tillage part to the side, the soil covering part comprises a scraping plate stretching across the fertilization ditch, and when the scraping plate moves forwards, the soil on the side, close to the edge of the fertilization ditch, of the second slide can be backfilled to the upper surface of the soil poured with bacterial manure. The biological fertilizer can be applied more reasonably, so that the fertilizer efficiency is greatly improved.

The invention provides bacillus pseudomycoides BP88 and application of the bacillus pseudomycoides BP88. The preservation number of the bacillus pseudomycoides BP88 is CCTCC (China Center For Type Culture Collection) NO.20241800. The invention further provides a preparation method of the bacillus pseudomycoides BP88. The bacillus pseudomycoides has a good disease prevention effect on tobacco root rot and has a remarkable growth promoting effect on tobacco plants. The strain has good theoretical research value and application prospect, can be used as a good test material for researching interaction and action mechanism of soil bacteria and plants, can also be used for research and development of soil bio-fertilizers and biological bactericides, can also become a good carrier of multifunctional engineering bacteria for disease prevention, growth promotion and the like, and has broad application prospects. And a new strain resource is provided for biological prevention and control of tobacco root rot.

The invention discloses a power grid tower footing area vegetation restoration method based on biological source humic acid and application of the power grid tower footing area vegetation restoration method, and belongs to the technical field of ecological restoration and soil treatment. The technology aims at solving the problems of soil erosion and loss caused by environmental disturbance conditions such as vegetation damage in power grid construction, and the technical scheme mainly comprises the steps of adopting a soil improvement technology based on HFA or combining the HFA with other bio-fertilizers for use, and configuring a formula for plants suitable for planting and a vegetation management strategy. The method mainly comprises the following steps: measuring various physical and chemical factors of a soil sample around a tower footing, comparing the physical and chemical factors with an average (normal) value of local soil, and formulating the HFA use amount and concentration according to the results; investigating site conditions such as local weather, terrain, soil properties and the like, native plants and suitable plants, and formulating a plant planting formula and a comprehensive vegetation management scheme capable of iteratively growing in different seasons. According to the method, the vegetation restoration speed and environmental protection are both considered, and vegetation restoration and ecological environment improvement can be rapidly achieved.

The invention relates to the field of wastewater treatment, and discloses an intelligent conditioning and purifying device for bio-fertilizer production wastewater, the intelligent conditioning and purifying device comprises a treatment tank body, the upper end of the treatment tank body is provided with a medicament adding port, the upper end of the inner side of the treatment tank body is provided with a pre-filtering plate, and the lower end of the inner side of the treatment tank body is provided with a top filtering plate; bottom filter plates are vertically and movably arranged below the top filter plates, and gaps between the top filter plates are used for being filled with activated carbon particles; a stirring part is arranged at the upper end of the pre-filtering plate. According to the intelligent conditioning and purifying device for the bio-fertilizer production wastewater, medicament adding treatment, pre-filtering treatment and activated carbon purification treatment are integrated on one device, and meanwhile, a gap between a top filter plate and a bottom filter plate can be increased during activated carbon particle adsorption treatment, and activated carbon particles can be dispersed; the use effect of the activated carbon particles is improved, and the activated carbon particles are blown by water treated at the bottom, so that circular wastewater treatment is realized, and the wastewater treatment effect is improved.

The invention discloses a pseudomonas capable of preventing and controlling citrus canker and promoting plant growth and application of the pseudomonas. The pseudomonas sp. CR1201 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on January 17, 2024, and the preservation number of the pseudomonas sp. CR1201 is GDMCC No: 64290. The pseudomonas sp. CR1201 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on January 17, 2024. The pseudomonas disclosed by the invention is a new species of the genus, can effectively antagonize citrus canker pathogen Xcc, and can promote plant growth by generating siderophores and plant growth hormone IAA. Therefore, the pseudomonas CR1201 disclosed by the invention can be used for preparing a microbial preparation product for preventing and treating the citrus canker pathogen, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting the growth and development of plants.

The invention relates to the technical field of bio-fertilizer crushing, and discloses a dynamic stress regulation and control method of a crusher in a bio-fertilizer production process, which comprises the following steps: arranging a non-uniform gradient microgroove array arranged according to a Fibonacci sequence on a working surface of a cutter, filling a beta-glucan-based viscoelastic composite material in the microgrooves, the grain orientation of a tool matrix is regulated and controlled through an electromagnetic field-assisted directional solidification process, and a dynamic stress wave self-interference regulation and control system is constructed. Through the synergistic effect of geometric arrangement of the microgroove array and the nonlinear damping characteristic of the viscous-elastic material, the destructive interference effect of stress waves is spontaneously formed in the crushing process, and space equalization and peak suppression of dynamic stress of the working face of the cutter are achieved; the problems of stress concentration and tool fatigue of a traditional crusher in high-fiber and high-viscosity bio-fertilizer raw material processing are fundamentally solved, the crushing efficiency is remarkably improved, and the service life of equipment is remarkably prolonged.

The invention relates to the technical field of crushing and crushing, and discloses a dynamic stress regulation and control method in a biological fertilizer crushing process, which comprises the following steps: injecting detection pulse airflow into a crushing cavity and collecting an acoustic reverberation signal of the detection pulse airflow; calculating an energy attenuation rate characteristic value from the signal, and dynamically adjusting an analysis frequency band and a regulation and control threshold value of the signal in combination with a material humidity and rotating speed signal obtained in real time; and finally, according to a comparison result of the characteristic value and a dynamic regulation and control threshold value, carrying out closed-loop regulation and control on operation parameters of the crusher. According to the method, a regulation and control mode for directly monitoring and regulating and controlling the energy utilization efficiency in the crushing cavity is constructed, the problem of hidden energy waste caused by excessive interaction or wall surface retention of the materials is avoided, the method can actively adapt to changes of the humidity of the materials and the rotating speed of equipment, and the regulation and control accuracy is guaranteed.

The invention relates to the technical field of tomato planting, in particular to a tomato carbon-based bio-fertilizer and application thereof in improvement of tomato quality. The invention provides a tomato carbon-based biological fertilizer. The tomato carbon-based biological fertilizer is prepared from the following components in parts by mass: 80-100 parts of a fermented organic fertilizer, 10-20 parts of a biological carbon soil conditioner, 3-5 parts of sorghum cake powder and 3-5 parts of corncob powder. From two aspects of providing nutrient substances and improving soil, the tomato carbon-based bio-fertilizer can provide sufficient and rich nutrient substances such as nitrogen, phosphorus and potassium, organic acid and the like for tomatoes, also has an effect of improving soil, and can provide a suitable growth environment for the tomatoes, so that the tomato carbon-based bio-fertilizer has a good application prospect. The tomato carbon-based bio-fertilizer provided by the invention can significantly improve the yield and quality of tomatoes.

The present invention relates to the technical field of agricultural fertilizers, and discloses a biofertilizer synthesized from Chinese herbal medicines, a preparation method and an application thereof, wherein the biofertilizer is prepared by combining herbal extracts such as astragalus, dandelion, forsythia, gallnut, and liquorice with Chinese herbal medicine residues such as honeysuckle residue, tobacco residue, malt residue, yeast residue, sophora flavescens residue, coptis chinensis residue, and stemonae residue, and is combined with organic fertilizers and nitrogen, phosphorus, and potassium fertilizers, and is fermented by composite microorganisms. The obtained biofertilizer is not only comprehensive in nutrients and easy to absorb, but also rich in natural antibacterial, anthelmintic substances and active microorganisms, which helps to promote crop growth and enhance disease and insect resistance. At the same time, long-term use can improve soil structure, enhance soil fertility and microecological balance, and reduce the use of chemical fertilizers and pesticides, thereby achieving the effects of increased production and efficiency, green environmental protection, and sustainable development.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses a special microbial fertilizer for cotton and a preparation method thereof, and belongs to the technical field of microbial fertilizers. The fertilizer is prepared from the following raw materials in parts by weight: 40-60 parts of a microbial agent, 80-100 parts of a microbial agent carrier, 40-60 parts of a nitrogen fertilizer, 30-50 parts of a phosphate fertilizer, 20-30 parts of a potassium fertilizer and 10-20 parts of a stabilizer, the microbial agent is prepared from nitrogen-fixing paenibacillus mucilaginosus MSSW01, bacillus nissi and streptomyces widermomole, and the microbial agent is prepared from bacillus mucilaginosus MSSW01 and streptomyces widermomole. The stabilizer is prepared from roxburgh rose extract, soybean protein isolate and gamma-aminobutyric acid according to the mass ratio of 2: 1: (0.2-0.5). By screening and optimizing three beneficial microbial strains and combining the synergistic effect of raw material components in a specific ratio, the yield and quality improvement of cotton can be remarkably promoted, and meanwhile, the occurrence rate of common diseases of cotton is effectively reduced.

The invention belongs to the technical field of biology, and particularly relates to a simple reniform worm enlarged culture and reniform worm cyst resuscitation method, which is characterized in that a wheat seedling culture solution is mixed with a reniform worm culture solution of escherichia coli, and the method comprises the following steps: carrying out small-range high-density culture on reniform worm; forming a reniform worm cyst and preserving breeds; resuscitating the reniform worm cyst; the resuscitated kidney-shaped worm can also be subjected to enlarged culture. The method is suitable for culture and cyst resuscitation of various kidney-shaped worms, the culture efficiency of the kidney-shaped worms is greatly optimized, the time required for the kidney-shaped worms to grow to the highest density is shortened, and resuscitation of dormant cysts of the kidney-shaped worms and the recovery speed of population density after resuscitation are both remarkably increased. The method can be applied to researches on morphological classification, cytobiology, ecology and the like of the reniform worm, research on environmental monitoring and treatment of the reniform worm in soil heavy metal pollution and the like, and research on preparation of microbial fertilizers from the reniform worm and the like.

The biological fertilizer processing and screening device comprises a box body, a scattering mechanism, a plurality of screening mechanisms, a plurality of vibration mechanisms, a driving mechanism and a collecting mechanism, feeding ports are formed in the two sides of the top face of the box body, the scattering mechanism comprises a grinding cone and two grinding blocks, the grinding cone is rotationally connected with the top wall of the box body, and the two grinding blocks are arranged on the top face of the box body. The two grinding blocks are connected to the two side walls of the box body respectively, the multiple screening mechanisms are sequentially arranged below the grinding cone from top to bottom, the vibration mechanism is arranged below the screening mechanisms, the driving mechanism is arranged on the box body and connected with the grinding cone and the vibration mechanism, and the collecting mechanism is arranged on one side of the box body. Therefore, caked fertilizer can be scattered, the caked fertilizer is prevented from blocking the screening net, and therefore the screening efficiency is effectively improved.

The bacillus velezensis MY-8 disclosed by the invention has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various plant pathogenic bacteria including cabbage alternaria alternata, rhizopus arrhizus, cladosporium very fine, fusarium tripteroides, alternaria alternata, Columbia cladosporium, botryosphaeria dothidea, fusarium oxysporum, geotrichum candidum, colletotrichum gloeosporioides and A. arundinis, and the bacillus velezensis MY-8 has an obvious inhibition effect on various plant pathogenic bacteria such as bacillus velezensis MY-8. The compound has a remarkable control effect on downy mildew, is expected to be prepared into a microbial preparation for biological control, and can be used as a biopesticide, a bio-fertilizer, a plant growth promoter and the like. The bacillus velezensis MY-8 disclosed by the invention can also express a structural domain containing protein of DUF4393, so that the bacillus velezensis MY-8 has potential bacteriophage infection resistance.

The utility model discloses a bio-fertilizer particle bacterium adding device, which particularly relates to the technical field of fertilizer production equipment and comprises a tank body, a motor is arranged at the top of the tank body, a bacterium adding hopper is arranged on one side of the motor, a bracket and a discharge pipe are arranged at the bottom of the tank body, and a feeding hopper is arranged on one side of the tank body. A stirring mechanism is arranged on the inner wall of the tank body. According to the biological fertilizer particle bacterium adding device, a stirring mechanism is arranged, a motor and a transmission rod are matched with each other to drive a partition plate worm and a scraper, so that when a bacterium scattering plate on the surface of a partition plate rotates to the position under a bacterium adding hopper, bacterium powder in the bacterium adding hopper can fall onto the surface of the bacterium scattering plate, and the bacterium powder is annularly and uniformly scattered in a tank body due to rotation of the partition plate; when the worm rotates to a bulge on the inner side of the track ring, the worm can be further pushed to slide into a sliding groove, and meanwhile, the worm is matched with a sliding rod and a spring, so that the worm moves left and right while rotating, the fertilizer particles are turned over, and the mixing effect is enhanced.

The invention relates to the field of agricultural microbial fertilizers, and discloses a composite biological bacterial fertilizer for promoting yield increase of kidney beans, which is prepared from chitosan modified zeolite, salt-tolerant growth-promoting bacteria, potassium-solubilizing and phosphate-solubilizing bacteria, plant signal bacteria and humic acid. The modified zeolite loads potassium ions, adsorbs redundant Na < + >, slowly releases potassium nutrients and adjusts the K < + > / Na < + > ratio of rhizosphere; the flora synergistically acts to secrete betaine, IAA and other substances, salt stress is relieved, and root development is promoted; the humic acid improves the soil structure and improves the water and fertilizer retention capability. Through a freeze-drying immobilization technology, the activity and durability of the flora are enhanced. The granular design of the bacterial fertilizer realizes slow release of nutrients and microorganisms, and improves the fertilizer efficiency and the application convenience. The saline-alkali soil conditioner is suitable for saline-alkali soil agricultural production, and has the effects of improving soil, relieving salt stress and promoting crop yield increase.

The invention discloses a method for preparing a biological fertilizer by combining compound probiotics and leucine fermentation liquor, and relates to the technical field of fertilizer preparation, the method comprises the following steps: carrying out magnetic reinforcement treatment on the probiotics, carrying out ultrasonic-assisted extraction on the leucine fermentation liquor, and activating and propagating the compound probiotics, and after the treatment on the compound probiotics and the leucine fermentation liquor is completed, carrying out ultrasonic-assisted extraction on the leucine fermentation liquor to obtain the biological fertilizer. The preparation method has the advantages that the bacterial liquid is placed in a magnetic field for pretreatment, so that the activity and the stability of the probiotics can be enhanced, the probiotics can play a better role in the subsequent fermentation and application process, the metabolic activity of the probiotics subjected to magnetic strengthening treatment is remarkably improved, and the metabolic activity of the probiotics subjected to magnetic strengthening treatment is remarkably improved. According to the invention, the leucine fermentation broth is used as a raw material, organic matters in the soil can be more effectively decomposed, more nutrients are released to be absorbed by crops, ultrasonic-assisted extraction is carried out on the leucine fermentation broth, and in the treatment process, ultrasonic treatment can destroy bacterial cells, so that leucine is more fully released, and the extraction rate and purity of leucine are improved.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a microalgae fertilizer capable of improving salt and alkali resistance of cucumbers, and the microalgae fertilizer is a composite microalgae bio-fertilizer and comprises scenedesmus obliquus, nitzschia grain and extracellular polymeric substances generated in the culture process of the nitzschia grain. The components of the microalgae compound fertilizer disclosed by the invention are all freshwater algae strains and natural active microalgae cells, so that sodium ions and metal cations cannot be accumulated, the microalgae compound fertilizer does not contain blue-green algae, and cytotoxin which is harmful to the environment cannot be generated; the used algae strains are easier to breed, and the biomass accumulation speed of the algae strains is far higher than that of other algae strains, so that the prepared microalgae biological fertilizer has the advantages of saving time and cost. And the polyunsaturated fatty acid generated by the microalgae fertilizer can enhance the plant resistance through an osmotic regulation system and an antioxidant enzyme ROS system.

The invention discloses bacillus velezensis B127 and application thereof, the bacillus velezensis is preserved in China Center for Type Microbiological Culture Collection, the preservation number is CCTCC NO: M 20252878, the preservation date is December 15, 2025, the classification name is bacillus velezensis B127, and the preservation address is Wuhan University, Hubei Province. The bacillus velezensis B127 has a remarkable inhibition effect on various tobacco fusarium pathogenic bacteria, has good phosphorus and potassium solubilizing capacity, generates hydrolase related to inhibition of pathogenic bacteria and generates siderophore and indoleacetic acid for promoting plant growth, is a biocontrol bacterium with excellent comprehensive performance, and can be widely applied to the field of biological control. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention relates to the technical field of fertilizers, in particular to a fulvic acid type compound microbial organic fertilizer and a preparation method thereof. The method comprises the following steps: preparing compound microorganism bacterium powder from bacillus subtilis, bacillus megatherium, bacillus licheniformis, pseudomonas fluorescens and bacillus psychrosaccharolyticus; preparing a solid-phase substance from ferric chloride hexahydrate, terephthalic acid and dimethylformamide; generating hydrogel on the surface of the chemical fertilizer in situ, and adding a solid-phase substance to obtain a slow-release chemical fertilizer; and preparing an organic fertilizer, and mixing the organic fertilizer with the compound microbial powder, the slow-release chemical fertilizer and the organic fertilizer to obtain the compound microbial organic fertilizer. According to the compound microbial organic fertilizer provided by the invention, the chemical fertilizer, the organic fertilizer and the microbial fertilizer synergistically promote the growth of plants, and the use of the chemical fertilizer is reduced.

The invention discloses a method and a system for optimally proportioning a microbial fertilizer protective agent and a preservative. The method is applied to a system comprising a cloud computing server, an edge computing node and a laboratory detection unit, environmental parameters such as temperature, humidity and pH value and bacterial activity data of the microbial fertilizer are collected through the edge computing node, and the data are uploaded to the cloud computing server. And the cloud computing server constructs a formula optimization model based on the collected data, calculates and outputs an optimal proportioning scheme, and controls the edge computing node to monitor and verify the bacterium activity stability of the optimal proportioning scheme. And dynamically adjusting the proportioning parameter of the protective agent and the preservative according to the verification result. The system integrates a high-precision sensor, data acquisition equipment and laboratory detection equipment, so that intelligent optimization of the ratio of the microbial fertilizer protective agent to the preservative is realized. The bacterial activity stability of the microbial fertilizer can be effectively improved, and the storage life is prolonged.