224 results about "Biofertilizer" patented technology

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses bacillus velezensis B127 and application thereof, the bacillus velezensis is preserved in China Center for Type Microbiological Culture Collection, the preservation number is CCTCC NO: M 20252878, the preservation date is December 15, 2025, the classification name is bacillus velezensis B127, and the preservation address is Wuhan University, Hubei Province. The bacillus velezensis B127 has a remarkable inhibition effect on various tobacco fusarium pathogenic bacteria, has good phosphorus and potassium solubilizing capacity, generates hydrolase related to inhibition of pathogenic bacteria and generates siderophore and indoleacetic acid for promoting plant growth, is a biocontrol bacterium with excellent comprehensive performance, and can be widely applied to the field of biological control. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention relates to a composite microbial fertilizer with a saline-alkali soil improvement effect and a preparation method thereof, and belongs to the technical field of microbial fertilizer preparation, the fertilizer comprises the following raw materials by mass: 10-30 parts of modified charcoal, 5-8 parts of a microbial agent, 30-50 parts of an inorganic fertilizer, 10-20 parts of a soil improvement agent and 3-5 parts of other auxiliary agents; wherein the modified biochar is obtained by performing segmented heat treatment modification on animal husbandry wastes; the microbial agent is obtained by immobilizing compound microorganisms by using sodium alginate gel reinforced by a mineral filler and micron-sized charcoal powder. The prepared fertilizer has the effects of improving crop quality and crop yield and improving saline-alkali soil.

The invention discloses a method for improving soil fertility of an orchard in a Yellow River old lane area. The orchard is covered with green grass; after the fruits are harvested and before leaves fall, a first fertilization ditch is dug at a crown water drip, and smashed straw, base fertilizer and surface soil are sequentially laid in the first fertilization ditch from bottom to top; meanwhile, digging 3-4 radial second fertilization ditches according to a water drip from a trunk to a crown, sequentially laying dry branches and fallen leaves, wheat straws and livestock and poultry manure in the second fertilization ditches from bottom to top, and putting earthworms into surface straws of the second fertilization ditches; a humic acid-containing water-soluble fertilizer is applied in a drip irrigation manner during topdressing. Therefore, through the synergistic effect of multiple technical measures such as orchard grass covering, organic fertilizer layer application, earthworm density increasing and bio-fertilizer application increasing, a benign soil ecological system is constructed, and therefore the problems that the content of organic matter in orchard soil in the Yellow River old road area is low, and the water and fertilizer retention capacity is weak are effectively solved, the soil fertility is remarkably improved, and the yield of the orchard is increased. And the fruit yield and quality are improved.

The invention relates to the technical field of agricultural microbial fertilizers, and discloses a soil improvement microbial fertilizer and a preparation method thereof, the soil improvement microbial fertilizer is prepared from the following raw materials by weight: 5-8 parts of a composite microbial agent, 30-40 parts of a modified biomass carrier, 25-35 parts of a slow release nutrient matrix, 15-20 parts of a soil conditioning aid, 2-3 parts of a microbial synergist, 1-2 parts of an adhesive, and 3 parts of a coating liquid. According to the invention, dual protection of trehalose loading and xanthan gum-PVA coating is adopted, and the L-proline synergist is matched, so that microorganisms still can survive in a high-temperature and high-salt environment, the inhibition of a severe soil environment on the microorganisms is broken through, and the application range is wider than that of commercially available products.

The invention discloses a preparation method of a liquid bio-fertilizer for sequestration of carbon by using microalgae. The preparation method comprises the following steps: S1, preparing components of the liquid bio-fertilizer for sequestration of carbon by using microalgae in parts by mass; s2, pretreating the raw materials; s3, carrying out a mixed reaction; step S4, carrying out homogenization treatment; s5, detecting and adjusting; and step S6, packaging a finished product. Nitrogen, phosphorus, potassium and other basic nutrients in the fertilizer and medium trace elements (calcium, magnesium, iron and zinc) form a basic nutrition system, and the microalgae carbon sequestration polysaccharide, the modified humic acid-microalgae protein compound and the nanoscale phosphorus-potassium chelate further achieve synergistic-slow release-targeted supply of the nutrients. The liquid biological fertilizer is long in fertilizer efficiency lasting period, reduces the fertilization frequency in the growth period of crops, and reduces the fertilization cost and labor intensity of farmers.

The present application relates to a kind of high nutrient high activity chemical fertilizer biological compound fertilizer and its preparation and application, belong to agricultural fertilizer technical field.The high nutrient high activity chemical fertilizer biological compound fertilizer of the present application includes inorganic fertilizer and microbial inoculant, the nitrogen phosphorus potassium nutrient content of the chemical fertilizer biological compound fertilizer is greater than 40%, and microbial viable count is above 200 million cfu / g, also includes microbial protective agent, the mass ratio of the microbial protective agent and microbial inoculant is ≥1.The high nutrient high activity chemical fertilizer biological compound fertilizer product of the present application realizes the deep compound of chemical fertilizer and active microorganism, has multiple efficacy of chemical fertilizer and microbial fertilizer, overcomes the neck blocking technical problem that chemical fertilizer and microbial fertilizer are incompatible in production, storage and transportation, use process, significantly improves chemical fertilizer utilization efficiency, reduces fertilizer use cost, solves the problem that microbial fertilizer cannot be generally used in field crops, integrates fertilizer yield and quality improvement, fertile soil and pollution prevention and other functions.

The utility model provides a bio-fertilizer screening device, which relates to the technical field of bio-fertilizer production and comprises a screening box, a movable plate is arranged in the screening box, a storage box is mounted at the top of the screening box, and an adjusting component is arranged on one side of the outer end of the screening box and used for adjusting the use position of the movable plate. The adjusting assembly comprises a fixing plate, an electric telescopic rod and a movable groove, and a conveying assembly is arranged between the storage box and the screening box and used for conveying the bio-fertilizer in the storage box into the screening box. According to the bio-fertilizer screening device, the use position of the movable plate can be adjusted through the adjusting assembly, so that the staggered position between the two screening holes can be adjusted, bio-fertilizer of different particle sizes can be screened through the screening holes, and the bio-fertilizer in the storage box can be quantitatively conveyed into the screening box through the conveying assembly; and the discharge amount of the bio-fertilizer from the screening box can be adjusted through an adjusting plate, so that the screening effect of the bio-fertilizer is ensured.

The invention provides a potassium-dissolving bacteria-iron-based potassium-containing nano material and a preparation method thereof, and belongs to a bio-fertilizer technology, and the preparation method comprises the following steps: dispersing soluble iron salt in water to obtain a solution A; dispersing a soluble phosphoric acid source and potassium salt in water to obtain a solution B; dispersing a stabilizer in water to obtain a solution C; simultaneously dropwise adding the solution A and the solution B into the solution C at the same speed, controlling the pH value to be 2.5-4.0 through alkali liquor, stirring for reaction, standing and aging to obtain the iron-based potassium-containing nano material; inoculating potassium-dissolving bacteria into a liquid culture medium, and carrying out enlarged culture to obtain a bacterial suspension; and mixing the bacterial suspension with the iron-based potassium-containing nano-material to obtain the potassium-dissolving bacteria-iron-based potassium-containing nano-material. The iron-based potassium-containing nano-material contains potassium, the iron-based potassium-containing nano-material and the potassium-dissolving bacteria act together to form a dual potassium supply mechanism that the potassium-dissolving bacteria decompose soil potassium and the iron-based potassium-containing nano-material releases potassium, and the effect is far better than that of single use of a microbial inoculum or a common potash fertilizer.

The utility model discloses a microbial fertilizer fermenting and mixing device with a quantitative discharging function, and relates to the technical field of microbial fertilizers. The microbial fertilizer fermenting and mixing device with the quantitative discharging function comprises a mixing tank, a mixing mechanism is arranged in the mixing tank, and a discharging mechanism is arranged above the mixing tank; the discharging mechanism is positioned above the mixing mechanism; the discharging mechanism comprises a discharging part and a pushing part; the blanking part comprises a storage box and a blanking pipe, and has the technical effects that the blanking mechanism drives a connecting rod through a control motor to enable a material leaking block to rotate, and quantitative repeated blanking is realized by utilizing whether a first material leaking groove in the material leaking block corresponds to a second material leaking groove in a material leaking plate or not; meanwhile, a connecting rod rotates to drive a reciprocating threaded rod through transmission of a worm sleeve and a worm gear, so that a material pushing frame reciprocates, microbial strains are prevented from being left in a material storage box, the addition amount of microbial fertilizer fermentation strains is accurately controlled, and the utilization rate of raw materials is increased.

The invention provides a wheat nitrogen fertilizer application reducing and effect increasing method, and belongs to the technical field of biological fertilizers. According to the method, under the background of straw returning to the field, arbuscular mycorrhizal fungi and mushroom residue and excrement decomposed fertilizer are combined as a main body, then peat soil and bentonite are added for wheat cultivation, and application reduction and efficiency improvement of wheat nitrogen fertilizer are achieved; wherein the arbuscular mycorrhizal fungi and the mushroom residue and excrement decomposed fertilizer are combined to promote soil straw degradation and assist nitrogen transfer, the peat soil and the bentonite can improve the water and fertilizer retention capacity of the soil and promote hypha growth and reproduction, available nitrogen in the soil is increased, soil nutrient loss is reduced, and the nutrient absorption capacity is enhanced; further, nitrogen fertilizer absorption and utilization of wheat are improved, and dependence on chemical fertilizer is reduced.

This utility model relates to the field of bio-fertilizer technology, providing a fermentation device and system. The fermentation device includes a fermentation tank and an aeration assembly. The aeration assembly includes an air pump, multiple microporous aeration discs, a ring-shaped pipeline, and a gas flow meter. The air pump provides oxygen to the fermentation broth. Multiple microporous aeration discs are located inside the fermentation tank. The ring-shaped pipeline is located at the bottom of the fermentation tank and is connected to each microporous aeration disc and the air pump. The gas flow meter is located in the ring-shaped pipeline and is used to detect the oxygen flow rate within the pipeline. The fermentation device provided by this utility model, by integrating the gas flow meter into the ring-shaped pipeline, monitors and feeds back gas flow data in real time. This allows operators to precisely adjust the air pump output based on quantitative indicators, avoiding the oxygen supply fluctuations caused by traditional reliance on experience-based judgment. This significantly lowers the operational threshold and provides a technical foundation for standardized control in large-scale production.

The utility model relates to the technical field of bio-fertilizer treatment equipment, in particular to photo-thermal insect repelling equipment which comprises a feeding mechanism, a photo-thermal insect repelling lamp, an insect receiving plate and a scouring insect repelling mechanism, a plurality of meshes allowing flies and insects to penetrate through are formed in the working face of the feeding mechanism, the photo-thermal insect repelling lamp is arranged above the feeding mechanism, and the insect receiving plate is arranged above the feeding mechanism. Under the action of light and heat emitted by the photo-thermal insect expelling lamp, flies and insects in a mixture conveyed by the feeding mechanism penetrate through meshes to escape downwards and fall onto the insect receiving plate, a cover body is further arranged on the feeding mechanism, and the cover body improves the acting effect on the flies and insects by gathering light and heat; the insect receiving plate is obliquely arranged, the flushing insect repelling mechanism is arranged above the insect receiving plate, the flushing insect repelling mechanism flushes water and / or blows air to the insect receiving plate, and therefore blockage caused by the fact that flies adhere to the insect receiving plate is avoided, the flies are transferred into the insect guide groove under the action of the oblique insect receiving plate and the flushing insect repelling mechanism, and the insect collecting effect is improved. And the insect is conveyed to the insect-water separation mechanism along the insect guide groove.

The present application belongs to the field of microbial technology, and particularly relates to a new bacterial strain of Acinetobacter sp. and its use. The bacterial strain is isolated from mangrove soil, is a new species of Acinetobacter sp., is named as Acinetobacter sp. GXMZU3951, and the bacteria have been registered and preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO: 36553. The Acinetobacter sp. GXMZU3951 of the present application has iron carrier production activity, can produce higher indole acetic acid, and has the ability to promote plant growth and the potential to resist heavy metals. The present application can be used in the biological remediation of heavy metal contaminated soil and as a green and environment-friendly biofertilizer for improving crop production, and has a good development and application prospect.

The fertilizer processing and screening equipment comprises a box body, a first-stage screen and a second-stage screen are arranged in the box body, the hole diameter of the first-stage screen is larger than that of the second-stage screen, the second-stage screen is located below the first-stage screen, and the included angle between the first-stage screen and the box body and the included angle between the second-stage screen and the box body are 30 degrees; a vibrating device is arranged on the inner surface of the box body, the output end of the vibrating device is fixedly connected with the first-stage screen and the second-stage screen, collecting holes are formed in the box body, screening of fertilizer particles is completed through the two-stage screen, chippings generated in the screening process are blown into the collecting net through an air blowing device, and the chippings are collected through the collecting net. And energy storage is performed through the inflator and the high-pressure air storage tank when the vibrator vibrates, so that particles attached to the screen can be cleaned without additional energy after overall screening is completed.

The invention discloses application of agrobacterium tumefaciens C53 in converting cow dung water into efficient liquid microbial fertilizer, and relates to the field of agricultural environment microbial technology and organic waste resource utilization, agrobacterium tumefaciens C53 bacterial liquid is inoculated into cow dung water, fermentation is carried out under the aerobic condition, and the liquid microbial fertilizer is obtained. The inoculation volume of the C53 bacterial liquid accounts for 20% of the total volume of the cow dung water. According to the strain C53, high-load cow dung water is transformed into a liquid fertilizer with better nutrient form and remarkable growth promoting effect, after the efficient liquid microbial fertilizer is applied to corn, the leaf weight and the root weight of the corn can be increased by about 48.9% and 58.8% respectively compared with blank control, and a feasible microbial technical scheme is provided for green and high-value resource utilization of breeding dung.

The present application belongs to the technical field of plant cultivation, and particularly relates to a biological fertilizer for relieving fruit drop of Taiwan long fruit mulberry and application. The biological fertilizer comprises animal manure, soybean meal powder, humic acid, peat soil, coconut bran, bran, fermentation liquor of a compound microbial agent, seaweed extract powder, amino acid chelated iron and borax; wherein the compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa. The above biological fertilizer is balanced in nutrients, is beneficial to enhancing the resistance of the mulberry to adversity and reducing fruit drop caused by stress; in addition, the Bacillus amyloliquefaciens and the Pseudomonas aeruginosa are used synergistically, and the two jointly build a healthy rhizosphere micro-ecology, thereby guaranteeing nutrient supply for fruit development from the root source. Therefore, the technical scheme of the present application can not only significantly reduce the fruit drop rate, but also improve the single fruit weight, soluble solid content and anthocyanin content of mulberry, and improve the flavor and color of the fruit.

The application provides a tea tree waste recycling method, which processes tea tree waste into a film for tea tree planting, or processes tea tree waste into a bio-fertilizer for tea tree planting, and the tea tree waste includes tea tree branches, tea leaves, tea dregs and tea stems. The method provided by the application performs special primary processing on the tea tree waste, and does not introduce too many chemical components and pesticides. Meanwhile, the method processes from soil to film combination, and has a 1+1>2 effect.

The invention discloses a sealed fermentation device for microbial fertilizer preparation, and relates to the technical field of fermentation devices.The sealed fermentation device comprises a fermentation box, a feeding port is fixed to the upper end of the fermentation box, a discharging valve port is fixed to the lower end of the fermentation box, an air cylinder is further fixed to the fermentation box, and a movable plate is fixed to the output end of the air cylinder; a motor is fixed to the lower end face of the movable plate, and an output shaft of the motor is in sliding connection with the fermentation box. According to the sealed fermentation device for preparing the microbial fertilizer, an air gap between a conductor rotor and a permanent magnet rotor is adjusted through an air cylinder, so that the rotating speed of a stirring plate can be changed, the stirring plate is driven to rotate by matching with transmission of a rack and a gear, and inclination angle adjustment of the stirring plate can be synchronously realized, so that low-viscosity materials can be stirred at high rotating speed and large inclination angle; the radial flow of the material is enhanced, the high-viscosity material is stirred at low rotating speed and small inclination angle, and the axial pushing is enhanced, so that the device is suitable for mixing the materials with different viscosities.

The invention discloses a method for preventing and treating sclerotinia rot of colza by antagonistic trichoderma strains, and relates to the technical field of agricultural disease prevention and control, and the method comprises the following specific steps: S100, strain screening and mutagenesis: separating trichoderma strains from rhizosphere soil of colza in different environments, primarily screening strains with antagonistic action on sclerotinia sclerotiorum by a plate confrontation method, and carrying out mutagenesis on the strains with antagonistic action on sclerotinia sclerotiorum; carrying out chemical mutagenesis treatment on the primarily screened strains, carrying out confrontation culture on the primarily screened strains and sclerotinia sclerotiorum again, screening out high-yield antagonistic substances with larger inhibition zones and mutant strains with strong stress resistance, and culturing to obtain trichoderma fermentation liquor; by obtaining the trichoderma strains which can keep efficient antagonism and strong stress resistance under different environmental conditions, the stability of the trichoderma strains on disease control is enhanced, so that the rape can effectively resist invasion of sclerotiniose under different environmental conditions in the growth process, and the disease resistance of the rape is improved. And antagonistic active substances are extracted to prepare a biological fertilizer carrier, and the biological fertilizer carrier is applied to the planting process of oilseed rape, so that the growth and reproduction of sclerotinia sclerotiorum are effectively inhibited.

The application provides a kind of biological fertilizer prepared based on blue-green algae mud, and belongs to the technical field of biological fertilizer preparation.The biological fertilizer according to the application comprises, by weight fraction, 20-40 parts of blue-green algae mud fermentation liquor, 30-40 parts of straw, 18-36 parts of animal manure, 5-8 parts of urea, 6-12 parts of ammonium chloride and 0.2-1 part of yeast, wherein the blue-green algae mud fermentation liquor is prepared by mixing blue-green algae mud and heat condensate, then adding enzyme preparation, mixed Bacillus and inorganic salt mixture, and anaerobic fermentation culture.The application not only realizes the resource treatment of blue-green algae mud and reduces environmental pollution, but also the prepared biological fertilizer contains nutrients such as nitrogen, phosphorus and potassium required for plant growth, and the crops applying the biological fertilizer prepared by the application can not only improve the survival rate, but also further improve the yield per mu, improve the soil quality, and the soil porosity reaches 60-65%, without caking and residue.

The utility model discloses an automatic pressure relief fermentation device for a bio-fertilizer, and relates to the technical field of bio-fertilizers. The bio-fertilizer fermentation device comprises a bio-fertilizer fermentation box arranged on a supporting frame, an access door is hinged to the bio-fertilizer fermentation box, a stirring component for stirring bio-fertilizer is arranged on the bio-fertilizer fermentation box, and the stirring component comprises a motor, a rotating shaft and a stirring rod. According to the utility model, the filter screen and the filter cartridge can play a role in blocking bio-fertilizer impurities, so that the situation that the bio-fertilizer impurities are wrapped by airflow to be discharged outwards to block the automatic pressure relief valve during pressure relief is avoided; meanwhile, the surfaces of a filter screen and a filter cartridge can be scraped and cleaned by a first cleaning component and a second cleaning component, so that the situation that the filter screen and the filter cartridge are blocked during pressure relief to influence discharge of airflow is avoided, normal pressure relief of the filter screen and the filter cartridge is ensured, and the situation that the bio-fertilizer fermentation box explodes due to overpressure caused by blockage of an automatic pressure relief valve is avoided.

The bacterial strain Arasana bacillus (CECT 30655) belongs to Gram-positive bacterial flora and bacillus subtilis, and is a stimulant capable of stimulating adaptive metabolism of plants to resist water stress, an improving agent for plant yield and nutrition and an improving agent for polyphenol content. The strain is separated from rhizosphere soil of pinus coast, is cultured on a nutrient agar culture medium (PCA), and is identified as a new strain from the aspects of morphology, biochemistry and genetics for identification through whole genome sequencing. Under a water stress condition, the strain can enhance CO2 fixation and transpiration, optimize photosynthesis energy capture and reduce oxidative stress, so that the strain can be used for increasing yield; as a biological fertilizer, nutrient absorption can be promoted no matter whether water stress exists or not; and the content of antioxidant polyphenols in plant species with medicinal or edible values can also be improved, so that the fruit quality is improved.

The invention discloses a granular bio-fertilizer and a preparation method thereof, and the granular bio-fertilizer comprises the following components in percentage by weight: 94-97% of a base fertilizer, 2-5% of a synergist and 1-2% of an auxiliary agent. The granular bio-fertilizer is a fertilizer prepared by compatibility of one of an inorganic fertilizer, an organic fertilizer and an organic and inorganic fertilizer with a bio-fertilizer synergist. The granular bio-fertilizer disclosed by the invention can adopt an inorganic fertilizer, an organic fertilizer or an organic and inorganic fertilizer as a base fertilizer, can be suitable for almost all granular fertilizers (except for fertilizers with a killing function on microorganisms or an antagonistic effect on components in a bio-fertilizer synergist) in the current market, and is simple in production process, relatively low in cost and suitable for industrial production. Moreover, the added value of the product can be effectively improved, and the production process belongs to a novel fertilizer production process and has a huge market popularization value.

The invention provides an application of a carbon dot nano enzyme in fruit and vegetable preservation and crop growth. The invention also provides a preparation method of the carbon dot nano-enzyme, and the carbon dot nano-enzyme is prepared by taking agricultural waste as a main carbon source, introducing a nitrogen doping agent and an antibacterial enhancer and synthesizing by adopting a hydrothermal method. The obtained carbon dot nano enzyme can be applied to fresh keeping of various fruits and vegetables in a foliage spraying or post-harvest treatment mode, the fresh keeping period is effectively prolonged, the weight loss rate is reduced, and nutritional ingredients are maintained; meanwhile, the carbon dot nano-enzyme can be used as a bio-fertilizer to promote crop growth through foliage spraying, and the crop yield is increased. The carbon dot nano-enzyme prepared by the invention is wide in raw material source, low in cost and simple in preparation process, has good biocompatibility and a multifunctional effect, meets the large-scale requirements of fruit and vegetable preservation and crop growth promotion, can remove active oxygen in crops, and is relatively high in practicability and beneficial to popularization.

The invention relates to the technical field of microorganisms, in particular to bacillus aryabhattai OF326 and application of the bacillus aryabhattai OF326 to preparation of bio-organic fertilizer and disease resistance, the bacillus aryabhattai OF326 is a probiotic screened by the applicant, research finds that the strain has the functions of IAA production, phosphorus solubilization and nitrogen fixation, growth of rice seedlings can be remarkably promoted, and the yield of the rice seedlings is increased. The method is suitable for secondary fermentation of organic fertilizers with sheep manure and traditional Chinese medicine residues as raw materials, and the viable count of beneficial bacteria is increased. In addition, tests show that the strain OF326 has a remarkable antagonistic effect on ralstonia solanacearum, and a clear antibacterial zone is formed; and by compounding with inositol or trehalose-6-phosphoric acid (T6P), the proliferation of the strain can be further promoted, and the growth rate of the strain is increased. In conclusion, the strain still keeps good growth-promoting and disease-resistant functions under the waterflooding condition, and is suitable for development of biological fertilizers and disease-resistant preparations.

The invention belongs to the technical field of plant cultivation, provides application of bacillus H8 in cultivation of emilia sonchifolia, and particularly relates to bacillus H8, a bacillus H8 suspension with the concentration of 102-104 CFU / mL is prepared and applied to the position 2-3 cm away from the root of emilia sonchifolia as a bio-fertilizer once every 7 days, plant growth can be remarkably promoted, and the yield of emilia sonchifolia is increased. The content of medicinal components of chlorogenic acid and quercitrin in the leaves is increased; the method solves the problems of soil degradation, unstable medicinal material quality and the like caused by dependence on chemical fertilizers and pesticides in traditional cultivation, is suitable for green and efficient cultivation of emilia sonchifolia, and has the advantages of simplicity and convenience in operation, environment friendliness, low cost and the like.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Erwinia gerundensis which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods.