55 results about "Botryosphaeria" patented technology

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY1. The lilium regale WRKY transcription factor gene LrWRKY1 has a nucleotide sequence shown as SEQ ID NO: 1, and codes a protein with a nucleotide sequence shown as SEQ ID NO: 2. According to the lilium regale Wilson WRKY transcription factor gene LrWRKY1, functional genomics related technical research verifies that the LrWRKY1 gene has the function of increasing the fungus resistance of plants; when the antifungal LrWRKY1 gene is constructed to a plant expression vector and is transferred to tobaccos for overexpression, transgenic tobacco plants have strong in-vitro antifungal activities, and experimental results show that the LrWRKY1 overexpressed transgenic tobaccos have obvious inhibiting effects on the growth of four fungi including botryosphaeria, sclerotinite, botrytis cinerea and fusarium oxysporum.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY4. The nucleotide sequence of the lilium regale WRKY transcription factor gene LrWRKY4 is described as in the SEQ ID NO:1,and the coded protein of the lilium regale WRKY transcription factor gene LrWRKY4 corresponds to the amino acid sequence described in the SEQ ID NO:2. According to the lilium regale WRKY transcriptionfactor gene LrWRKY4, it is proved that the LrWRKY4 gene has the function of improving the antifungal ability of plants through the technical research related to the functional genomics, the antifungal LrWRKY4 gene is constructed to a plant expression vector and transferred into tobacco for overexpression, the transgenic tobacco has very high antifungal activity, and the experimental result showsthat the tobacco with the LrWRKY4 gene overexpressed is highly resistant to infestation of nigrospora oryzae, fusarium graminearum, fusarium graminearum, botryosphaeria and fusarium solanum.

The invention discloses botryosphaeria dothidea LAMP (Loop-mediated Isothermal Amplification) detection primers and application thereof. A primer composition for detecting botryosphaeria dothidea based on an LAMP technology consists of 6 specific primers, namely F3, B3, FIP, BIP, LF and LB. The invention further provides a botryosphaeria dothidea LAMP detection method. The botryosphaeria dothideaLAMP detection method comprises the following steps: 1), extracting DNA of a sample to be detected; 2), performing loop-mediated isothermal amplification on the DNA of the sample to be detected by using the primer composition provided by the invention; 3), performing result detection, wherein when a chromogenic result is green fluorescence or a trapezoidal electrophoretic band appears, a positiveresult is determined, and when a chromogenic result is orange or orange yellow or no trapezoidal electrophoretic band appears, a negative result is determined. A novel molecular detection method and the novel primer composition are provided for the botryosphaeria dothidea, the botryosphaeria dothidea can be detected quickly, conveniently and efficiently at high specificity and high sensitivity, and a reliable technological and theoretical basis is provided for early diagnosis, detection and rapid import and export inspection of the botryosphaeria dothidea.

The invention discloses a Chinese wild pepper plant mosquito-repellent incense capable of improving sleep and a preparation method thereof. The Chinese wild pepper plant mosquito-repellent incense disclosed by the invention is compounded by adopting natural traditional Chinese medicines, is environment-friendly, safe without toxic or side effect and high in safety property, not only is capable of effectively repelling mosquitoes, but also achieves certain inhibition and killing effects on multiple pathogens and fungi, namely staphylococci, mycobacterium tuberculosis, pseudomonas aeruginosa, gram-negative bacteria, gram-positive bacteria and the like, is aromatic in smell, moderate in property, fresh and capable of tranquilizing mind and relieving pressure, can effectively improve the sleep without causing adverse effect on human bodies and is suitable for people of all ages.

The invention discloses a radix notoginseng mitogen-activated protein kinase kinase gene PnMAPKK1.A nucleotide sequence of the gene PnMAPKK1 is as shown in SEQ ID NO:1, and the gene PnMAPKK1 codes mitogen-activated protein kinase kinase.According to functional genomics related technical researches, the gene PnMAPKK1 has a function of improving pathogenic fungus resistance of plants.After the antifungal gene PnMAPKK1 is constructed to a plant expression vector and transferred into tobaccos to realize overexpression, transgenic tobacco plants have extremely high in-vitro antifungal activity.The transgenic tobacco plants with PnMAPKK1 overexpressed have an evident inhibition effect on growth of fusarium solani, colletotrichum gloeosporioides, verticillium fusarium and botryosphaeria.

The invention discloses a drug for preventing and treating gummosis. The drug contains the following ingredients in parts by weight: 10-15 parts of 50% alkaloid-gardenoside, 8-12 parts of 70% Baume-degree lime-sulfur mixture, 10-15 parts of 70% mancozeb wettable powder, 4-8 parts of icariin, 4-8 parts of hypericin, 6-10 parts of forsythin, 4-8 parts of bupleurum roots and 6-10 parts of artemisia annua. The drug is simple in proportion and convenient in use, and the treatment effect on peach tree gummosis is good; shown by testing, the solution of a volume which is 400-600 times that of a mixture has an obvious inactivation effect on botryosphaeria and peach physalospora (belonging to ascomycotina) and has a good bactericidal effect.

The invention relates to the field of light chemical engineering and aims at providing Pseudofusicoccum sp. F10 and printing and dyeing application thereof. The Pseudofusicoccum sp. F10 can produce an indigo blue pigment, the pigment is a compound with C-CH3 substituents and containing para-aminobenzoic acid, the biggest absorption peak of the pigment in a neutral and weakly-alkaline water solution is located at the position of 620 nm, the pigment precipitates in an acidic water solution and is not sensitive to oxidants, and a reducing agent sodium sulfite has the damage effect on the pigment. During dyeing and printing application, the pigment has the good printing and dyeing effect on cotton fabrics, and a mordant NaCl is conducive to the dyeing effect. The novel natural dye obtained by adopting the Pseudofusicoccum sp. F10 is safe, is environmentally friendly and is not limited by regions or environments, the fungal pigment has good reproducibility, the yield is increased remarkably by adopting a biological cultivation method, and industrialization is easier to achieved.

The invention discloses a sustained-release calamus electric heating liquid mosquito-repellent incense and a preparation method thereof. The preparation method comprises the following steps: stirring, dispersing and uniformly mixing the following materials in parts by weight: 40-60 parts of ethanol, 10-30 parts of butyl butyrate, 10-15 parts of traditional Chinese medicine mosquito-repellent purified solutions, 18-19 parts of eucalyptus oil, 1-2 parts of isopropyl myristate, 10-15 parts of fatty alcohol-polyoxyethylene ether sodium sulfate and 0.1-0.3 part of sodium benzoate, and then filling into a bottle; inserting a volatilizing rod made from fibers into the bottle, and heating so as to volatilize to repellent mosquitoes. The sustained-release calamus electric heating liquid mosquito-repellent incense disclosed by the invention is high in safety, not only is capable of effectively repelling the mosquitoes, but also achieves certain inhibition and killing effects on multiple pathogens and fungi, such as staphylococci, mycobacterium tuberculosis, pseudomonas aeruginosa, gram-negative bacteria and gram-positive bacteria, is fragrant in smell, moderate in property, fresh and capable of tranquilizing mind and relieving pressure, can effectively improve the sleep and enhance the sleep quality and is suitable for people of all ages.

The invention discloses a biocontrol strain for preventing and treating poplar canker and an application method thereof. The strain is trichoderma aureoviride, with number of CGMCC No.8891. Fermentation liquor of the strain is mixed with wheat bran and corn powder, the product is naturally air-dried and ground to prepare raw powder after fermentation for seven days, and 38wt% of raw powder, 38wt% of kaolin, 13wt% of sodium lignosulphonate, 8.5wt% of SDS and 2.5wt% of humic acid are added to prepare an agent. After being diluted, the agent is used for preventing and treating the poplar canker by spraying and root irrigation. The strain has the advantages of various modes of action on pathogenic bacteria, high reproduction speed, stable action effects, and the like, has strong inhibiting effects on the pathogenic bacterium botryosphaeria dothidia of the poplar canker, is of great importance in prevention and control of the poplar canker and has good development and application prospects.

The invention discloses a papaya Chinese herba preparation for treating chronic gastroenteritis and a preparation method thereof. The papaya Chinese herba preparation is prepared from the following raw materials in parts by weight: 200-220 parts of papaya, 40-60 parts of rhizoma coptidis, 50-70 parts of yam, 55-75 parts of hawthorns, 60-80 parts of wild chrysanthemum flowers, 55-75 parts of radix paeoniae alba, and 65-85 parts of honey. The preparation method comprises the following steps: firstly, cutting papaya, yam and hawthorns into slices, and drying and dehydrating the slices; then, mixing the obtained slices with rhizoma coptidis, wild chrysanthemum flowers and radix paeoniae alba, and decocting the obtained mixture, extracting by using 95% ethanol, after the obtained product is subjected to standing and liquid separation, concentrating the obtained product; and finally, adding honey, mixing, heating and modulating the obtained product, after the obtained product is cooled, sequentially carrying out fine filtration and ultraviolet disinfection, and carrying out vacuum metering and bottling on the obtained product, so that the papaya Chinese herba preparation for treating chronic gastroenteritis is obtained. A cydonic acid contained in the papaya adopted by the preparation disclosed by the invention has a strong anti-inflammatory and antibacterial effect, and has a significant inhibitory effect on various intestinal bacteria and staphylococcus; and supplemented by effective drugs for treating acute enteritis, the preparation can effectively treat diseases caused by acute enteritis.

The invention discloses an application method of disease resistant drugs for peach tree gummosis. The application method comprises the following steps: A. materials of 50% of superfine carbendazim wettable powder, 70% of superfine thiophanate-methyl wettable powder, 72% of Dubangkelu wettable powder, stachydrine, hypericin, total anthraquinones, shuanghuangliang and isatis root are prepared; B. an extracting solution of the shuanghuangliang and the isatis root is digested by alcohol; C. the 50% of superfine carbendazim wettable powder, the 70% of superfine thiophanate-methyl wettable powder, the 72% of Dubangkelu wettable powder, the stachydrine, the hypericin and the total anthraquinones are compounded with the extracting solution of the step B for evenly stirring, and are subjected to ultraviolet sterilization to obtain drugs resisting to pseudomonas solanacearum. The application method disclosed by the invention has the advantages of simplicity, simple application method of used products, simple preparation of the used products, convenience in use and good treatment effect on the peach tree gummosis; through the test of experiments, 400-600 times of liquid of the mixture has an obvious inactivation effect on botryosphaeria and peach capsule spore fungus (belonging to ascomycotina fungus); the sterilization effect is good.

The invention discloses an electric heating liquid mosquito-repellent incense with sweet marjoram fragrance and a preparation method thereof. The preparation method comprises the following steps: stirring, dispersing and uniformly mixing the following materials in parts by weight: 40-60 parts of ethanol, 10-30 parts of butyl butyrate, 10-15 parts of traditional Chinese medicine mosquito-repellent purified solutions, 18-19 parts of eucalyptus oil, 1-2 parts of isopropyl myristate, 10-15 parts of fatty alcohol-polyoxyethylene ether sodium sulfate and 0.1-0.3 part of sodium benzoate, and then filling into a bottle; inserting a volatilizing rod made from fibers into the bottle, and heating so as to volatilize to repellent mosquitos. The electric heating liquid mosquito-repellent incense disclosed by the invention is high in safety, not only is capable of effectively repelling the mosquitoes, but also achieves certain inhibition and killing effects on multiple pathogens and fungi, such as staphylococci, mycobacterium tuberculosis, pseudomonas aeruginosa, gram-negative bacteria and gram-positive bacteria, is fragrant in smell, moderate in property, fresh and capable of tranquilizing mind and relieving pressure, can effectively improve the sleep and enhance the sleep quality and is suitable for people of all ages.

The invention discloses a biocontrol strain for preventing and treating poplar canker and an application method thereof. The strain is fusarium equiseti, with number of CGMCC 8795. Fermentation liquor of the strain is mixed with wheat bran and corn powder, the product is naturally air-dried and ground to prepare raw powder after fermentation for seven days, and 42wt% of raw powder, 36wt% of kaolin, 12wt% of Tween 20, 8wt% of nekal and 2wt% of ascorbic acid are added to prepare an agent. After being diluted, the agent is used for preventing and treating the poplar canker by spraying and root irrigation. The strain has the advantages of various modes of action on pathogenic bacteria, high reproduction speed, stable action effects, and the like, has strong inhibiting effects on the pathogenic bacterium botryosphaeria dothidia of the poplar canker, is of great importance in prevention and control of the poplar canker and has good development and application prospects.

The invention discloses streptomyces carpaticus with the preservation number being CCTCC NO: M2019120 and taxonomically named as Streptomyces carpaticusn T33. The test shows that the streptomyces carpaticus can inhibit plant pathogenic bacteria, and has obvious antagonistic effects on banana anthracnose, mango anthracnose, pearl plum anthracnose, botryosphaeria, banana fusarium oxysporum No. 4 andalternaria alternata. Therefore, the streptomyces carpaticus can be used for biological control, the strain and the fermentation liquor of the streptomyces carpaticus have potential value of developing plant pathogenic bacteria inhibitors and have good application prospects. In addition, research discovers that the fermentation liquor of the strain can enhance the disease resistance of banana seedlings and remarkably promote growth of banana plants, and can be used as a banana growth promoter. In summary, the streptomyces carpaticus with biological control and banana growth promoting characteristics has important significance for safe and sustainable development of the agriculture.

The invention discloses an electric heating liquid mosquito-repellent incense with mint fragrance and a preparation method thereof. The preparation method comprises the following steps: stirring, dispersing and uniformly mixing the following materials in parts by weight: 40-60 parts of ethanol, 10-30 parts of butyl butyrate, 10-15 parts of traditional Chinese medicine mosquito-repellent purified solutions, 18-19 parts of eucalyptus oil, 1-2 parts of isopropyl myristate, 10-15 parts of fatty alcohol-polyoxyethylene ether sodium sulfate and 0.1-0.3 part of sodium benzoate, and then filling into a bottle; inserting a volatilizing rod made from fibers into the bottle, and heating so as to volatilize to repellent mosquitoes. The electric heating liquid mosquito-repellent incense disclosed by the invention is high in safety, not only is capable of effectively repelling the mosquitoes, but also achieves certain inhibition and killing effects on multiple pathogens and fungi, such as staphylococci, mycobacterium tuberculosis, pseudomonas aeruginosa, gram-negative bacteria and gram-positive bacteria, is fragrant in smell, moderate in property, fresh and capable of tranquilizing mind and relieving pressure, can effectively improve the sleep and enhance the sleep quality and is suitable for people of all ages.

The invention discloses effector protein from botryosphaeria dothidea and an encoding gene and application thereof. The effector protein is the protein having one of the following amino acid residue sequences: (1) the protein formed by an SEQ ID No. 1 amino acid residue sequence in a sequence list; (2) the protein which is derived from SEQ ID No. 1 by carrying out substitution and / or deficiency and / or addition of one or several amino acid residues on the SEQ ID No. 1 amino acid residue sequence, and has a pathopoiesis-related effector protein function. Through a transgenosis experiment of the effector protein shows that the encoding gene has a function of motivating anti-disease genes in tobacco, and a hypersensitive necrosis reaction of the tobacco can be caused; in addition, the pathogenicity of the botryosphaeria dieback can be enhanced after overexpression, and the effector protein is an important target for researching the anti-disease genes.

The invention discloses a biocontrol Bacillus thuringiensis strain and an application thereof. The collection number of Bacillus thuringiensis BCN10 is CGMCC No: 16675. Fermentation liquor of the Bacillus thuringiensis effectively inhibits common loquat surface putrefying fungi (fusarium oxysporum, botryosphaeria parva, trichoderma aureoviride, colletotrichum gloesporioides and penicillium expansum) on a bi-zone flat plate and a loquat fruit. Volatile substances generated by the fermentation liquor are applied to corrosion resistance in loquat storage, natural putrefaction disease time of theloquat fruit can be delayed, and incidence rate is decreased. Volatile antibacterial substances generated by the Bacillus thuringiensis BCN10 can avoid direct contact with the fruit, the fruit is preserved at normal temperature, food storage time is prolonged, and the strain can be used for preparing a biocontrol preservative which is used for inhibiting the putrefying fungi such as the fusarium oxysporum, the botryosphaeria parva, the trichoderma aureoviride, the colletotrichum gloesporioides and the penicillium expansum and has a wide antibacterial spectrum.

The invention provides a toy disinfectant. The toy disinfectant is prepared from Herba Typhonii, Caulis Sargentodoxae, Semen Torreyae, Bulbus Lycoridis Aureae, Rhizoma Pteridii Latiusculi, Semen Sterculiae Nobilis, Fructus Tubocapsici Anomali, Fructus Ulmi Macrocarpae, Daphne genkwa Sieb. et Zucc., Rhizoma Paridis, Herba seu Radix Delphinii Grandiflori, Herba Urenae Procumbentis, Dryopteris setosa (Thunb.) Akasawa and Pteris multifida. The toy disinfectant has the advantages that prepared from natural medicinal materials by means of elaborate extracting, the toy disinfectant is targeted at eliminating bacteria on toys of children and capable of inhibiting staphylococcus, salmonella, candida albicans, pneumobacillus, escherichia coli, proteusbacillus vulgaris and the like; the toy disinfectant prepared from the natural medicinal materials avoids threat to health of the children and is efficient, non-irritating, environment friendly and free of toxic or side effect while sterilization and cleaning are guaranteed, thereby being applicable to various wood toys, plastic toys, leather toys and the like.

The invention discloses a drug for resisting peach tree gummosis. The drug contains the following ingredients in parts by weight: 10-15 parts of 50% ultramicro carbendazim wettable powder, 8-12 parts of 70% ultramicro thiophanate-methyl wettable powder, 10-15 parts of 72% Dupont curzate wettable powder, 4-8 parts of stachydrine, 4-8 parts of hypericin, 6-10 parts of total anthraquinone, 4-8 parts of double coptis chinensi and 6-10 parts of radix isatidis. The drug is simple in preparation and convenient in use, and the treatment effect on peach tree gummosis is good; shown by testing, the solution of a volume which is 400-600 times that of a mixture has an obvious inactivation effect on botryosphaeria and peach physalospora (belonging to ascomycotina), and has a good bactericidal effect.

The invention discloses a compound screened by a target protein sterol 14alpha-demethylase three-dimensional structure, and an application thereof in the preparation of a bactericide. A result of indoor drug sensitivity measurement proves that the screened compound 1-[1-(3-chlorobenzoyl)piperidine-4-yl]azacycloheptane has a good inhibition activity on a main plant pathogen fungus Fusarium graminearum causing wheat scab diseases, and also has a good inhibition activity on Botrytis cinerea causing fruit and vegetable gray molds and Botryosphaeria berengriana causing apple ring rot. The application of the green small-molecule compound 1-[1-(3-chlorobenzoyl)piperidine-4-yl]azacycloheptane screened by the target protein three-dimensional structure in the prevention and control of plant diseasesis provided for the first time, so the compound has a broad market application prospect.

A medicine for external use for nursing ears, noses and throats and a preparing method thereof are disclosed. The medicine is prepared from menthol, sodium chloride, glycerin, dipotassium glycyrrhizinate, a traditional Chinese medicine extract product and distilled water. The traditional Chinese medicine extract product is prepared from wild chrysanthemum flower, crocus sativus, baical skullcap root, common rush, herba houttuyniae, canscora lucidissima, purslane, white peony root, herba barleriae lupulinae, asparagus cochinchinensis, anoectochilus roxburghii, turmeric, lotus stamen and iris, and is prepared by mixing the traditional Chinese medicine raw materials according to a ratio, adding water, decocting and extracting twice, combining two filtrates, allowing the mixture to stand, filtering, adding other raw materials according to a ratio, fully stirring, purifying, filtering for filtration, subpackaging, and sterilizing with ultraviolet ray. The medicine is reasonable in formula and good in cleaning effects, has good treating effects on skin infections of ears, nasal cavities and throats, can effectively inhibit biological streptococcus, staphylococcus, bacillus influenza, influenza viruses, and other pathogenic bacteria and viruses, and is safe and nontoxic, thus effectively preventing and treating skin infections of ears, nasal cavities and throats and protecting skin safety of human bodies.

The present invention relates to an antiviral bacteria-killing cleaning detergent with several health-care functions. Said cleaning detergent is made up by using the raw materials of water, edible acetin, edible vinegar, garlic, onion, clove, cinnamon bark, nutmeg, origanum, hot pepper, grape, thymus mongolicus, gleditsia fruit and sorbate through a traditional preparation process. Said invention also provides its application range.

The invention discloses a manufacturing method for a gummosis bacteria prevention drug. The manufacturing method comprises the following steps: A. preparing materials: preparing 50 percent Liang Guoan, 70 percent baume lime sulphur, 70 percent mancozeb wettable powders, icariin, hypericin, phillyrin, radix bupleuri and artemisia carvifolia; B. carrying out alcohol leaching for extracting solutions of radix bupleuri and artemisia carvifolia; C. compounding the 50 percent Liang Guoan, the 70 percent baume lime sulphur, the 70 percent wettable mancozeb powders, the icariin, the hypericin and the phillyrin with the extraction solutions obtained from Step B, stirring the compound uniformly, and carrying out ultraviolet sterilization to obtain the drug for pseudomonas solanacearum resistance. The manufacturing method provided by the invention is simple, that is, the manufacturing method for the manufactured product is simple, and the proportioning of the manufactured product is simple; the use is convenient; the treatment effect for peach tree gummosis is remarkable; a 400-times to 600-times diluent of the mixture is proved, by the experiments, to have obvious inactivation effects for botryosphaeria and peach physalospora (belonging to Ascomycotina in the kingdom Fungi), and the sterilizing effect is good.

The invention relates to a fructus evodiae total alkaloids extraction method. The method comprises the follow steps of smashing fructus evodiae into coarse powder and adding water into the coarse powder to prepare suspension; homogenizing the suspension to obtain fructus evodiae homogenate; processing the fructus evodiae homogenate step by step, then adding 75% ethyl alcohol, and sealing for freezing, thus obtaining supernate; drying the supernate under reduced pressure to obtain a fructus evodiae total alkaloids extract. The method is high in extract yield, short in production flow and easy to industrialize; in addition, the extract has an obvious restraining effect on staphylococcus aureus, helicobacter polyri, pseudomonas aeruginosa, drug-resistant staphylococcus epidermidis and escherichia coli.

The invention relates to the field of light chemical industry and aims to provide a strain Pseudofusicoccum sp. F10 of the genus Botrytis and its application in printing and dyeing. The fungus F10 can produce indigo pigment, which is a compound with C-CH3 substituents and aromatic acids. The maximum absorption peak of the pigment is located at 620nm in neutral and slightly alkaline aqueous solutions, and the pigment is precipitated in acidic aqueous solution; The oxidizing agent is not sensitive, and the reducing agent sodium sulfite can destroy the pigment; the printing and dyeing effect on cotton fabrics is good, and the mordant NaCl is helpful for the dyeing effect. The novel natural dye obtained by the invention is safe and environment-friendly, and the invention is not limited by region and environment, the reproducibility of the fungal pigment is good, and the biological cultivation method greatly increases the output, and it is easier to realize industrialization.

The invention relates to a rapid physalospora piricola virus detection technique. According to difference of different ITS sequences of physalospora piricola virus registered in GenBank, specific physalospora piricola virus primers BsF1 / BsR2 are designed by using Primer5 software, the primers can only amplify a 332bp strip specifically from only 22 test Botryosphaeria / berengeriana strains, and not any strip is amplified from other strains and blank control strains. The invention further provides a detection method of the primer for the physalospora piricola virus and can detect a sample DNA with lag quantity. The detection method has the advantages of good specificity, high sensitivity, short time, and the like, and plays an important role in in-time detection and effective control on diseases.

The invention relates to the technical field of biocontrol bacteria, in particular to an ear botryosphaeria HN17496 strain, a biocontrol microbial inoculum as well as a preparation method and application of the biocontrol microbial inoculum. The preservation number of the strain HN17496 disclosed by the invention is CCTCC (China Center For Type Culture Collection) NO: M2022489. The grape ear mold HN17496 strain provided by the invention has a very good biocontrol effect on wheat root rot and bihilum, and has no potential safety hazard to people and livestock; according to the embodiment of the invention, the inhibition rate of the ear mold HN17496 strain and the metabolite of the ear mold HN17496 strain on wheat root rot bihilum spore is 65%-72%, and the biocontrol effect is remarkable.

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.