Lilium regale WRKY transcription factor gene LrWRKY4 and application thereof

A transcription factor and gene technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems affecting the yield and quality of cut lily flowers, scale rot and shedding, and the quality of seed bulbs, and achieve broad market application prospects and breeding cycles. The effect of shortening, reducing usage
CN110734482AActive Publication Date: 2020-01-31KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2020-01-31

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Abstract

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY4. The nucleotide sequence of the lilium regale WRKY transcription factor gene LrWRKY4 is described as in the SEQ ID NO:1,and the coded protein of the lilium regale WRKY transcription factor gene LrWRKY4 corresponds to the amino acid sequence described in the SEQ ID NO:2. According to the lilium regale WRKY transcriptionfactor gene LrWRKY4, it is proved that the LrWRKY4 gene has the function of improving the antifungal ability of plants through the technical research related to the functional genomics, the antifungal LrWRKY4 gene is constructed to a plant expression vector and transferred into tobacco for overexpression, the transgenic tobacco has very high antifungal activity, and the experimental result showsthat the tobacco with the LrWRKY4 gene overexpressed is highly resistant to infestation of nigrospora oryzae, fusarium graminearum, fusarium graminearum, botryosphaeria and fusarium solanum.
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Description

Technical field

[0001] The invention relates to the research fields of molecular biology and genetic engineering related technologies, in particular to a Minjiang lily WRKY transcription factor gene LrWRKY4 and applications. Background technique

[0002] Plants will be subject to a variety of abiotic or biotic stresses during their growth and development. Abiotic stresses include drought, waterlogging, salinity, lack of nutrients, low temperature, radiation, etc. Biological stresses include fungi, bacteria, viruses, nematodes or parasitism. Seed plants etc. Among biotic stresses, fungi cause the largest number of plant diseases, accounting for more than 70%, seriously harming plant growth and economic value. Fungi are tenacious and many pathogenic fungi can form special tissues or spores to overwinter and are harmful all year round. Fungi spread easily, mainly through airflow and water flow in fields; in addition, wind, rain, and insects can also spread fungal diseases. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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