83 results about "Fungal gene" patented technology

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY1. The lilium regale WRKY transcription factor gene LrWRKY1 has a nucleotide sequence shown as SEQ ID NO: 1, and codes a protein with a nucleotide sequence shown as SEQ ID NO: 2. According to the lilium regale Wilson WRKY transcription factor gene LrWRKY1, functional genomics related technical research verifies that the LrWRKY1 gene has the function of increasing the fungus resistance of plants; when the antifungal LrWRKY1 gene is constructed to a plant expression vector and is transferred to tobaccos for overexpression, transgenic tobacco plants have strong in-vitro antifungal activities, and experimental results show that the LrWRKY1 overexpressed transgenic tobaccos have obvious inhibiting effects on the growth of four fungi including botryosphaeria, sclerotinite, botrytis cinerea and fusarium oxysporum.

The invention discloses a lilium regale gene Lr14-3-3 with antifungal activity. The gene Lr14-3-3 has the nucleotide sequence as shown in SEQ ID NO:1 and encodes protein 14-3-3. A relevant technology of functional genomics proves that the gene Lr14-3-3 has a function of improving the plant antifungal activity. The antifungal gene Lr14-3-3 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; the transgenic tobacco plant has strong in vitro antifungal activity; and the transgenic tobacco expressing the Lr14-3-3 has obvious inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and alternaria.

A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.

The invention relates to regulators of fungal gene expression and their use in commercial and medical applications. More particularly, the invention relates to regulators of fungal genes involved in production of enzymes, secondary metabolites and other useful products, as well as to regulators of genes involved in fungal invasion. The invention provides novel regulators of fungal gene expression, and methods for using regulator genes in commercial and medical applications.

The invention relates to a fungus colony PCR method and a pathogenic fungus identification method. The fungus colony PCR method aims to achieve the high positive rate of amplification, and the pathogenic fungus identification method aims to achieve short detection time and reliable results. The fungus colony PCR method comprises the following steps of: inoculating fungus cultures onto a culture medium for culture; taking fungus colonies which begin to appear on the culture medium as experimentally available colonies; and preparing a DNA template for colony PCR amplification. The pathogenic fungus identification method comprises the following steps of: inoculating clinical sample cultures or pathogenic fungus cultures onto the culture medium for culture; taking the colonies which begin to appear on the culture medium as the experimentally available colonies; preparing the DNA template for the PCR amplification; and detecting amplification products. The fungus colony PCR method and the pathogenic fungus identification method have the advantages of improving the positive rate of the fungus colony PCR amplification, solving the problems of complex steps, wall-breakage difficulty, longtime and the like in fungus genomic DNA extraction, performing colony identification at the very beginning of the growth of pathogenic fungi, and achieving reliable results and identification time remarkably shorter than conventional fungus phenotype identification time.

The invention discloses a susceptible fungal gene LrWRKY-S1 of lily and application thereof. A nucleotide sequence of the LrWRKY-S1 gene is shown by SEQ ID NO.1 and an amino acid sequence coded thereby is shown by SEQ ID NO.2. Biological functions of the protein are studied by a bacteriostasis experiment. Experimental results show the protein makes plants more susceptible to pathogenic fungi suchas Botrytis cinerea, fusarium oxysporum and Colletotrichum orbiculare. As found as well, the LrWRKY-S1 gene has a low expression level in lilium regale with high resistance, and a homologous gene thereof has high expression levels in lilium davidii, lilium longifolorum and lilium lancifolium with important economic values. The gene and the application thereof provide an efficient susceptible genefor genetic engineering reconstruction, lays a foundation for further cultivation of fungus-resistant / susceptible lily or new materials or new species of other plants, can provide theoretical and technical support for later application of the gene in plant genetic improvement, broadens ideas for prevention and control of plant diseases and has very extensive application values.

The present invention provides nucleic acid amplification assays for mutations to two short sections of the fungal gene FKS1. Mutations in these target sequences have been shown to correlate with resistance to echinocandin-class drugs. Assays may include detection by sequencing or by labeled hybridization probes. Also, primers, probes and reagent kits for performing such assays.