Primer and probe for fast detecting various fungi and identifying strains and application of primer and probe

A fungal and rapid technology, applied in the field of microbial detection, can solve the problems of difficulty in distinguishing the specificity of colonized bacteria and infectious bacteria, laboratory contamination, false positives, etc., and achieve the effect of simple operation, high cost, high throughput, and strong specificity
CN106987626AActive Publication Date: 2017-07-28HANGZHOU DIAN BIOTECH CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HANGZHOU DIAN BIOTECH CO LTD
Publication Date
2017-07-28

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Abstract

The invention discloses a primer, molecular beacon and kit for fast detecting various clinically common fungi and identifying strains and belongs to the technical field of microbiological detection. The sequences of the primer is as shown in SEQ ID No. 1-4, and the sequences of the detecting molecular beacon is as shown in SEQ ID No. 5-7. The primer and the molecular beacon have the advantages that the specific primer and the molecular beacon are designed at conserved sites by comparing the gene sequences of various clinically common fungi, and the primer and the molecular beacon can fast detect and identify 8 clinically common candida, 1 cryptococcus, aspergillus, mucor and penicillium. The kit for detecting various fungi contains the primer and the molecular beacon and can fast and accurately detect various clinically common fungi.
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Description

technical field

[0001] The invention belongs to the technical field of microbial detection, and in particular relates to a primer, a molecular beacon and a kit for rapidly detecting a variety of common clinical fungi and identifying bacterial species. Background technique

[0002] Mycosis is a disease caused by fungi as pathogens. It includes not only superficial fungal infections that invade the cuticle, hair, and nail plate, but also deep mycoses that invade the dermis, subcutaneous tissue, and internal organs. In recent years, with the extensive use of broad-spectrum antibacterial drugs, glucocorticoids, immunosuppressants and various medical catheters, the development of organ transplantation and long-term treatment of chronic diseases such as tumor treatment, the invasiveness of immunocompromised people and hospitals The incidence of fungal infections has a clear upward trend, mainly caused by Candida, Cryptococcus and Aspergillus, some rare fungi and opportunistic path...

Claims

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