924 results about "Dermis" patented technology

Aspects of the current subject matter are directed to a wearable analyte monitoring device with an integrated applicator for deployment of a microneedle array-based sensor into a skin surface of a subject. The microneedle array is retained within a housing body of the wearable analyte monitoring device in a first, loaded configuration. Upon actuation, a biasing element accelerates the microneedle array into the skin of the subject, transitioning the microneedle array to a second, deployed configuration in which the microneedle array protrudes through a distal opening of the housing body and enabling sensing by the microneedle array of one or more target analytes in dermal interstitial fluid of the subject.

Described herein are methods and apparatuses for reducing or eliminating skin glands (e.g., sebaceous, eccrine and apocrine) with an electric treatment. These apparatuses and methods may produce electroporation of cells by applying treatment dose having an energy density sufficient to eliminate or reduce the size of a target gland. Also described herein are methods for treating and / or preventing a disorder of a skin gland. For example, described herein are methods of treating sebaceous hyperplasia.

There is disclosed a packaged allograft implant configured for implantation in a human recipient. In an embodiment, the packaged allograft implant includes an acellular dermal matrix sheet having a top surface and a bottom surface in opposition to one another, a perimeter surrounding the top surface and the bottom surface, and a thickness extending between the top surface and the bottom surface. The packaged allograft implant further includes a mesh pattern extending across at least a portion of the top surface and the bottom surface of the acellular dermal matrix sheet, the mesh pattern providing through-holes extending between the top surface and the bottom surface of the acellular dermal matrix sheet, and the mesh pattern having a plurality of mesh lines extending in a first direction and a second direction. The first direction and the second direction are orthogonal to one another so as to allow a given amount of stretch in each of the first direction and the second direction. Other embodiments are also disclosed.

The invention belongs to the field of cosmetic detection, and discloses a cosmetic and cosmetic raw material sunscreen function evaluation method and application. According to the evaluation method, a sunscreen effect test is carried out on a test sample by using a non-contact administration device, wherein the test sample is a cosmetic finished product or a diluted cosmetic raw material; the test comprises evaluation of in-vitro sunscreen effect by skin cells, evaluation of in-vivo sunscreen effect by a zebra fish embryo model and result evaluation. According to the method, the sunburn-resistant effect is evaluated through expression of beta-galactosidase in human dermal fibroblasts and / or secretion of collagen, the sunburn-resistant effect is evaluated through the survival rate of human keratinocytes, the DNA damage condition or inflammatory factor secretion and the lethal / teratogenic condition of zebra fish embryos, and multi-layer and all-around sunburn-resistant effect evaluation is achieved. The method provided by the invention is suitable for evaluating the sunscreen function of various sunscreen products, has accuracy and practicability, and provides an important technical basis for research and development, quality control and performance optimization of sunscreen cosmetics.

The invention provides a radiotherapy patient skin injury real-time detection record management system based on image processing, and belongs to the technical field of radiotherapy patient skin injury real-time detection. Comprising a multispectral image acquisition module for imaging the skin of a patient at the same visual angle by adopting different spectrums to obtain three spectral images including near-infrared imaging, infrared imaging and ultraviolet imaging; a first identification module is used for fusing three spectral images to obtain an image data set, and a second identification module is used for constructing an epidermis injury coefficient # imgabs0 #, a dermis injury coefficient # imgabs1 # and a subcutaneous injury coefficient # imgabs2 # of the ith patient and evaluating the epidermis injury coefficient # imgabs0 #, the dermis injury coefficient # imgabs1 # and the subcutaneous injury coefficient # imgabs2 # respectively. A skin injury evaluation model is constructed, the comprehensive injury area ratio is calculated, the classification of injury levels is realized, data support is provided for a personalized treatment scheme, and a powerful auxiliary diagnosis tool is provided for a medical institution.

The invention belongs to the technical field of tissue engineering, and particularly discloses a preparation method of a 3D full-thickness skin model. The preparation method provided by the invention comprises the following steps: constructing a corium layer, constructing an inner epidermal layer through first epidermal inoculation, constructing an epidermal layer through second epidermal inoculation and obtaining a complete 3D full-thickness skin model through co-culture. The 3D full-thickness skin model obtained through the preparation method is distinct in gradation, uniform in thickness, free of shrinkage and stable in quality, the epidermal layer and the corium layer are uniformly fused, and the 3D full-thickness skin model is suitable for skin function research and sample evaluation. Meanwhile, the preparation method provided by the invention is simple in process and convenient to operate, does not need additional reagents or anti-shrinkage tools, can meet the requirements of large-scale industrial production, and has good production feasibility and market application prospects.

The invention discloses a skin chip, a skin model and application of the skin chip and the skin model. The material of the skin chip does not contain polydimethylsiloxane; the skin chip comprises a first chip and a second chip, wherein the first chip is used for providing a bottom accommodating space for a dermal culture medium; the dermal culture chip layer and the epidermal culture chip layer are arranged at the top end of the first chip, and the second chip is used for supplying and storing a culture medium or supplying skin irritants; wherein the skin chip adopts a gravity perfusion mode; the skin chip is detachable, and all layers of the skin chip are connected through a polyimide material or are made of the polyimide material; a culture cavity between the dermal culture chip layer and the epidermal culture chip layer is through up and down; the first chip and the second chip are not provided with electrodes, or the first chip and the second chip are respectively provided with a first electrode and a second electrode. According to the skin chip provided by the invention, the experiment flexibility is enhanced, and the cost is reduced; and a real culture environment can be simulated.

The invention provides a novel human-derived II-type collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The invention further provides a preparation method of the novel human-derived II-type collagen COLII-14K-H. The invention also provides a preparation method of the human-derived II-type collagen COLII-14K-H and application of the human-derived II-type collagen COLII-14K-H in preparation of cartilage repair drugs, skin repair drugs and skin care products. The inventor-derived II-type collagen can obviously improve the gene expression level of the collagen, so that the content of the collagen in human dermal fibroblasts is improved, and the skin aging is delayed; meanwhile, the protein fragment can show a remarkable skin repairing effect at low concentration, provides a new treatment choice for skin repairing, and can be widely applied to the fields of skin repairing and nursing.

The present invention belongs to the field of cosmetics detection, and discloses a method for evaluating the sun protection function of cosmetics and cosmetic raw materials and its application. This evaluation method uses a non-contact drug delivery device to test the sun protection effect of the test samples, and the test samples are finished cosmetics or diluted cosmetic raw materials; the tests include evaluating the in vitro sun protection efficacy by skin cells, evaluating the in vivo sun protection efficacy by the zebrafish embryo model, and result evaluation. This method evaluates the anti-aging effect of sun protection through the expression of β-galactosidase and / or the secretion of collagen in human dermal fibroblasts, and evaluates the sunburn prevention effect through the survival rate of human keratinocytes, DNA damage, or the secretion of inflammatory factors and the lethal / teratogenic conditions of zebrafish embryos, realizing multi-level and all-round evaluation of the sun protection efficacy. The method provided by the present invention is applicable to the evaluation of the sun protection function of various sun protection products, has accuracy and practicability, and provides an important technical basis for the research and development, quality control, and performance optimization of sun protection cosmetics.

The present invention discloses a probiotic postbiotic composition for improving the scalp environment, a preparation method thereof and an application thereof, wherein the probiotic postbiotic composition for improving the scalp environment comprises postbiotics obtained by fermenting two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602; the Lactobacillus paracasei ProSci-101 has a preservation number of CGMCC No.26528; the Lactobacillus fermentum ProSci-602 has a preservation number of CGMCC NO.25451. The probiotic postbiotic composition for improving the scalp environment of the present invention has the effects of cell repair, enhancing elastin and renewing the metabolism of the dermis, can slow down cell aging, and promote the proliferation of stratum corneum cells; thereby effectively achieving the purpose of improving the scalp environment and preventing hair loss.

The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.

The invention provides a decellularized dermal matrix particle and a preparation method thereof, the preparation method comprises the steps of pretreatment, degreasing, virus inactivation, decellularization, grinding, crosslinking, freeze-drying and sterilization subpackaging, and solves the problems that the existing decellularized particle product is non-uniform in particle size, incomplete in decellularization, poor in injection effect and incapable of meeting the requirements of tissue repair and regeneration. The invention develops a product which is short in production cycle, relatively concentrated in particle size distribution, thorough in cell removal and complete in matrix function preservation, can effectively remove fat and viruses in animal skin, and has the advantages of being thorough in decellularization, short in time, high in efficiency, light in damage to dermal matrix, capable of removing antigens, capable of retaining a natural structure and capable of effectively guiding tissue regeneration. When the temperature of the freeze-dried particle dispersion liquid is reduced, the water binding capacity of the particles is reduced, free water in the prepared solution is increased, the particles naturally float and gather (dense at the upper part and sparse at the lower part), and the free water is in the lower layer and still has good dispersity after being sterilized.

The invention relates to the field of cosmetics, and relates to a collagen-containing cosmetic, in particular to application of a reverse micelle carrier in preparation of a cosmetic for improving collagen intradermal retention volume. The cosmetic comprises a collagen composite raw material, the collagen composite raw material comprises the following components in percentage by mass: 53.55-80% of grease; 15 to 60 percent of phospholipid; 0.5 to 1.5 percent of an antioxidant; 0.1 to 2 percent of water; and 0.01% to 0.5% of collagen; the grease is prepared from any one of isopropyl myristate, isopropyl laurate or isopropyl acetate; the phospholipid comprises lecithin, and the purity of the lecithin is 90% or above. The retention amount of the collagen in the cosmetic containing the collagen in the skin can be increased, so that the collagen permeates into the skin epidermis or dermis and accumulates in the skin layer; the real collagen supplementing effect of cosmetics is greatly improved, and the skin young state is facilitated.

The invention relates to the technical field of gene engineering, in particular to application of a detection reagent for a hair follicle development related gene of a down producing goat. According to the present invention, the Cashmere goat hair follicle development related gene is any one or more of lncRNA MSTRG.20890.1, chi-miR-24-3p and ADAMTS3, and the lncRNA MSTRG.20890.1 and the ADAMTS3 are competitively combined with the chi-miR-24-3p, such that the significant differential expression of the lncRNA MSTRG.20890.1 in the Cashmere goat secondary hair follicle morphogenesis process is found, and the results prove that the lncRNA MSTRG.20890.1 can regulate the proliferation and the migration of dermal fibroblasts through the competitive combination of the lncRNA MSTRG.20890.1 and the ADAMTS3, such that the growth of the Cashmere goat secondary hair follicle morphogenesis can be inhibited therefore, the formation of a dermal papilla structure is inhibited.

The invention discloses an anti-aging composition which comprises DNA (deoxyribonucleic acid) sodium, enzyme-digested hyaluronic acid and madecassoside, and the mass ratio of the DNA sodium to the enzyme-digested hyaluronic acid to the madecassoside is (0.01-1): (0.01-1): (0.01-0.5). The invention also provides a preparation method and application of the anti-aging composition, cosmetics and skin care products. The anti-aging composition acts on epidermal cells and dermal cells at the same time, collagen synthesis and hyaluronic acid synthesis can be improved, the effects of barrier repairing and skin filling are achieved, and the purpose of multi-dimensional anti-aging is achieved.

The invention discloses arginine grafted sodium hyaluronate gel as well as a preparation method and application thereof in beauty, and belongs to the technical field of biomedical materials. The arginine-grafted sodium hyaluronate gel is prepared by the following method: mixing a BDDE cross-linking agent with a sodium hydroxide solution, then adding arginine-grafted sodium hyaluronate, carrying out cross-linking, and after the cross-linking is completed, putting a product into a phosphate buffer solution for swelling; and after swelling is completed, homogenizing and sterilizing to obtain the arginine grafted sodium hyaluronate gel. The gel prepared by the invention has excellent thermal stability, overcomes the defects that arginine and sodium hyaluronate are unstable in action and easy to wash off in the prior art, so that the prepared gel can play long-acting moisturizing, anti-inflammatory and repairing roles after being injected into the dermal superficial layer, thereby showing a better application prospect in the field of medical cosmetology.

The invention discloses a quantitative detection method for sweat gland cholinergic nerves of a neuron intracellular inclusion body disease. Belongs to the technical field of biological science. Performing immunohistochemical staining; scanning a slide; and calculating the density of the cholinergic nerve fiber. The invention relates to a simple and feasible method for quantitatively evaluating cholinergic nerve fibers of a neuron intracellular inclusion body disease. The method comprises the following steps: carrying out skin biopsy on control receptors of a neuron intranuclear inclusion body disease and a nervous system-free disease, slicing, carrying out immunofluorescent staining by using a VIP antibody, quantitatively calculating the density of cholinergic nerve fibers labeled by the sweat gland VIP antibody in dermis by using Image J software, analyzing whether the cholinergic nerve fibers have differences in the two groups of receptors, and if so, determining whether the cholinergic nerve fibers have differences in the two groups of receptors. Evaluating the nervous injury and severity of the cholinergic nerve; the invention provides a new way for accurately evaluating the autonomic nerve injury and severity of the neuronal intracellular inclusion body disease.

The invention discloses an extracellular matrix fusion protein as well as a preparation method and application thereof, relates to the technical field of recombinant proteins, and aims to solve the technical problems that protein cosmetics in the market are relatively single in effect and difficult to meet application requirements. According to the extracellular matrix fusion protein, the nucleotide sequence of the extracellular matrix fusion protein is as shown in SEQ ID No.7. According to the extracellular matrix fusion protein with the nucleotide sequence shown as SEQ ID No.7, an in-vitro cell test proves that the fusion protein can remarkably promote cell adhesion, proliferation and cell migration, can act on a corium layer in a transdermal mode and remarkably promote collagen secretion, and due to the fact that recombinant protein belongs to biological protein, the fusion protein can be used for preparing the extracellular matrix fusion protein with the nucleotide sequence shown as SEQ ID No.7. Compared with common chemical cosmetics and hormone drugs, the composition has the advantages of easiness in decomposition, no residue and the like, and has a wider application prospect.

Described herein are compositions for promoting hair growth and / or treating or preventing hair loss in a mammalian subject. In certain embodiments, the mammalian subject is a human male or a human female. Methods of making and using the same are also described. The compositions in various embodiments may comprise: a tea extract which may comprise a polyphenol, e.g., epigallocatechin gallate; an extract from Larix europaea which may comprise the flavonoid taxifolin; a natural ingredient that antagonizes or mitigates the proliferation of Gram-positive bacteria in the hair shaft passageway; compounds including zinc chloride and / or hexamidine diisethionate; follicle stimulator. e.g., caffeine, an ingredient that improves the health of the dermis. e.g., sodium hyaluronate; and / or ingredients specifically tailored for male or female subjects, e.g., Serenoa serrulata fruit extract or extract of Pterocarpus marsupium.

Embodiments treat inflammation of a user by applying a first patch on a dermis of the user adjacent to an afferent nerve, the first patch comprising first electrodes, and applying a second patch on the dermis of the user adjacent to a vagus nerve or a vagus nerve connecting branch, the second patch comprising second electrodes. Embodiments generate a treatment protocol during a time period comprising simultaneously applying a first electrical stimuli to the afferent nerve via the first electrodes of the first patch and applying a second electrical stimuli to the vagus nerve or the vagus nerve connecting branch via the second electrodes of the second patch.

The present invention relates to a peptide having an activity of promoting hair growth or preventing or inhibiting hair loss. The peptides of the present invention have the activity of promoting the proliferation of hair follicle dermal papilla cells (HFDPC), activating cell proliferation-related signaling proteins in human dermal papilla cells, activating beta-catenin, improving the expression level of beta-catenin downstream factors, down-regulating the expression level of alopecia protein DKK-1, and increasing the expression of keratin in hair outer root sheath cells (HORSC).

The present disclosure relates to a method to obtain a decellularized dermal matrix from a rabbit, hare or beaver skin graft, the method comprising the following steps: degreasing the skin graft using a hydrocarbon solvent, perchloroethylene, modified alcohols or supercritical carbon dioxide, or combinations thereof; liming the degreased graft by immersing the graft in a solution comprising calcium hydroxide, sodium hydroxide, hydrogen peroxide, or mixtures thereof; fleshing the skin graft; optionally, deliming the skin graft; incubating the skin graft in a solution of ethylenediamine tetraacetic acid; treating the skin graft with a detergent solution for 30 minutes to 3 hours to obtain the decellularized dermal matrix. The disclosure also relates to a decellularized dermal matrix obtained by the method described; to a composition comprising said decellularized cell matrix for use in medicine or veterinary medicine; and to dermo-epidermal skin substitute scaffold comprising the disclosed decellularized dermal matrix.

The invention discloses a scar-removing microneedle body and a scar-removing microneedle composite patch, and belongs to the technical field of microneedle patches, the scar-removing microneedle composite patch comprises a patch layer and a microneedle layer, the patch layer comprises a silica gel layer and a substrate layer, and the substrate layer is arranged between the microneedle layer and the silica gel layer; the microneedle layer comprises a plurality of microneedle bodies, each microneedle body comprises an epidermal segment and a dermis segment, and the epidermal segment is composed of a matrix and a first scar removing component; the dermis segment is composed of a matrix and a second scar removing component; the scar-removing microneedle body is of a two-section structural design, two scar-removing components are combined, different scar-removing components of epidermal layer targeting and corium layer targeting are accurately provided to a target skin layer, individual scar-removing components can be concentrated on the target skin layer, and the scar-removing effect is improved. Meanwhile, side effects or interference possibly generated by scar removing components on different skin layers can be avoided, and the precise and efficient compound scar removing effect is achieved.

The invention relates to the technical field of cosmetics, in particular to a composition, a reagent, application of the composition and the reagent, a generation accelerant of type I collagen and type III collagen and cosmetics. The composition is prepared from the following components in parts by weight: 0.001 to 0.55 part of palmitoyl tripeptide trifluoroacetate, 0.001 to 1 part of methylsilanetriol and 0.001 to 0.55 part of snake venom-like tripeptide. According to the technical scheme provided by the invention, not only can the mitochondrial function be adjusted, but also the expression of HSP47 protein can be promoted, so that the collagen generation of dermis is promoted, the length and the quantity of nasolabial folds are reduced, the skin elasticity is improved, and a good anti-aging effect is shown.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The present invention relates to a method for preparing an acellular dermal matrix derived from a human body, in which the acellular dermal matrix is washed for the second time with an alcohol-based degreasing solution, thereby not only having excellent safety against cytotoxicity, but also being able to effectively remove residual fat.

The invention discloses Dong'e donkey-hide gelatin active peptide and application thereof. Relates to the technical field of biology. The invention provides an amino acid sequence and application of the Dong'e donkey-hide gelatin active peptide. The amino acid sequence of the CCA active peptide is YYTSASGDEMVSLK, and the CCA active peptide can significantly up-regulate the I-type collagen gene expression, increase the I-type and III-type collagen content and reduce senescence markers. The peptide fragment can be combined with TGFBR2, especially TGFBR1, gene and protein expression of the TGFBR2 can be up-regulated, downstream targets of a TGF-beta-SMAD pathway can be activated, and the MMPs / TIMPs ratio can be reduced. According to the present invention, the TGF beta-SMAD pathway is activated, the MMPs / TIMPs ratio is reduced, the collagen synthesis is promoted, and the excessive decomposition of the collagen is inhibited, such that the I type collagen content and the III type collagen content are increased, and the aging degree of the photoaging dermal fibroblast is reduced;

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention relates to the technical field of cosmetics, in particular to an efficient anti-aging composition and a skin care product containing the composition. The invention discloses an efficient anti-aging composition which is prepared from the following raw materials in parts by mass: 12-15 parts of oil-based concentrated active ingredients, 25-35 parts of base collagen liquid, 15-18 parts of Ectoine, 12-16 parts of TWEEN 20 and 22-25 parts of isosorbide dimethyl ether, and the oil-based concentrated active ingredients are prepared from safflower seed oil, an extract of flowers / leaves / stems of herba lysimachiae, an extract of radix astragali membranaci, ubiquinone and vitamin E; the base collagen liquid comprises XVII type collagen, VII type collagen and IV type collagen. Aiming at the problems that an existing skin care product cannot effectively reduce skin wrinkles, improve skin compactness, reduce skin sagging and prevent skin aging for a long time, the composition disclosed by the invention realizes long-term aging resistance from epidermis to dermis, effectively reduces wrinkles, improves compactness and improves skin sagging.

The invention provides a Dendrobium officinale Kimura amp (MIG) leaf extract, a preparation method of the Dendrobium officinale Kimura amp (MIG) leaf extract, and application of the Dendrobium officinale Kimura amp (MIG) leaf extract in the fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 have the effect of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, HDFa), and can be used for preparing anti-aging drugs or skin care products, so that the dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 can be used for preparing anti-aging drugs or skin care products.