737 results about "Dermis" patented technology

The invention provides a radiotherapy patient skin injury real-time detection record management system based on image processing, and belongs to the technical field of radiotherapy patient skin injury real-time detection. Comprising a multispectral image acquisition module for imaging the skin of a patient at the same visual angle by adopting different spectrums to obtain three spectral images including near-infrared imaging, infrared imaging and ultraviolet imaging; a first identification module is used for fusing three spectral images to obtain an image data set, and a second identification module is used for constructing an epidermis injury coefficient # imgabs0 #, a dermis injury coefficient # imgabs1 # and a subcutaneous injury coefficient # imgabs2 # of the ith patient and evaluating the epidermis injury coefficient # imgabs0 #, the dermis injury coefficient # imgabs1 # and the subcutaneous injury coefficient # imgabs2 # respectively. A skin injury evaluation model is constructed, the comprehensive injury area ratio is calculated, the classification of injury levels is realized, data support is provided for a personalized treatment scheme, and a powerful auxiliary diagnosis tool is provided for a medical institution.

The invention belongs to the technical field of tissue engineering, and particularly discloses a preparation method of a 3D full-thickness skin model. The preparation method provided by the invention comprises the following steps: constructing a corium layer, constructing an inner epidermal layer through first epidermal inoculation, constructing an epidermal layer through second epidermal inoculation and obtaining a complete 3D full-thickness skin model through co-culture. The 3D full-thickness skin model obtained through the preparation method is distinct in gradation, uniform in thickness, free of shrinkage and stable in quality, the epidermal layer and the corium layer are uniformly fused, and the 3D full-thickness skin model is suitable for skin function research and sample evaluation. Meanwhile, the preparation method provided by the invention is simple in process and convenient to operate, does not need additional reagents or anti-shrinkage tools, can meet the requirements of large-scale industrial production, and has good production feasibility and market application prospects.

The present invention belongs to the field of cosmetics detection, and discloses a method for evaluating the sun protection function of cosmetics and cosmetic raw materials and its application. This evaluation method uses a non-contact drug delivery device to test the sun protection effect of the test samples, and the test samples are finished cosmetics or diluted cosmetic raw materials; the tests include evaluating the in vitro sun protection efficacy by skin cells, evaluating the in vivo sun protection efficacy by the zebrafish embryo model, and result evaluation. This method evaluates the anti-aging effect of sun protection through the expression of β-galactosidase and / or the secretion of collagen in human dermal fibroblasts, and evaluates the sunburn prevention effect through the survival rate of human keratinocytes, DNA damage, or the secretion of inflammatory factors and the lethal / teratogenic conditions of zebrafish embryos, realizing multi-level and all-round evaluation of the sun protection efficacy. The method provided by the present invention is applicable to the evaluation of the sun protection function of various sun protection products, has accuracy and practicability, and provides an important technical basis for the research and development, quality control, and performance optimization of sun protection cosmetics.

The present invention discloses a probiotic postbiotic composition for improving the scalp environment, a preparation method thereof and an application thereof, wherein the probiotic postbiotic composition for improving the scalp environment comprises postbiotics obtained by fermenting two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602; the Lactobacillus paracasei ProSci-101 has a preservation number of CGMCC No.26528; the Lactobacillus fermentum ProSci-602 has a preservation number of CGMCC NO.25451. The probiotic postbiotic composition for improving the scalp environment of the present invention has the effects of cell repair, enhancing elastin and renewing the metabolism of the dermis, can slow down cell aging, and promote the proliferation of stratum corneum cells; thereby effectively achieving the purpose of improving the scalp environment and preventing hair loss.

The invention provides a decellularized dermal matrix particle and a preparation method thereof, the preparation method comprises the steps of pretreatment, degreasing, virus inactivation, decellularization, grinding, crosslinking, freeze-drying and sterilization subpackaging, and solves the problems that the existing decellularized particle product is non-uniform in particle size, incomplete in decellularization, poor in injection effect and incapable of meeting the requirements of tissue repair and regeneration. The invention develops a product which is short in production cycle, relatively concentrated in particle size distribution, thorough in cell removal and complete in matrix function preservation, can effectively remove fat and viruses in animal skin, and has the advantages of being thorough in decellularization, short in time, high in efficiency, light in damage to dermal matrix, capable of removing antigens, capable of retaining a natural structure and capable of effectively guiding tissue regeneration. When the temperature of the freeze-dried particle dispersion liquid is reduced, the water binding capacity of the particles is reduced, free water in the prepared solution is increased, the particles naturally float and gather (dense at the upper part and sparse at the lower part), and the free water is in the lower layer and still has good dispersity after being sterilized.

The invention discloses an extracellular matrix fusion protein as well as a preparation method and application thereof, relates to the technical field of recombinant proteins, and aims to solve the technical problems that protein cosmetics in the market are relatively single in effect and difficult to meet application requirements. According to the extracellular matrix fusion protein, the nucleotide sequence of the extracellular matrix fusion protein is as shown in SEQ ID No.7. According to the extracellular matrix fusion protein with the nucleotide sequence shown as SEQ ID No.7, an in-vitro cell test proves that the fusion protein can remarkably promote cell adhesion, proliferation and cell migration, can act on a corium layer in a transdermal mode and remarkably promote collagen secretion, and due to the fact that recombinant protein belongs to biological protein, the fusion protein can be used for preparing the extracellular matrix fusion protein with the nucleotide sequence shown as SEQ ID No.7. Compared with common chemical cosmetics and hormone drugs, the composition has the advantages of easiness in decomposition, no residue and the like, and has a wider application prospect.

Embodiments treat inflammation of a user by applying a first patch on a dermis of the user adjacent to an afferent nerve, the first patch comprising first electrodes, and applying a second patch on the dermis of the user adjacent to a vagus nerve or a vagus nerve connecting branch, the second patch comprising second electrodes. Embodiments generate a treatment protocol during a time period comprising simultaneously applying a first electrical stimuli to the afferent nerve via the first electrodes of the first patch and applying a second electrical stimuli to the vagus nerve or the vagus nerve connecting branch via the second electrodes of the second patch.

The present invention relates to a peptide having an activity of promoting hair growth or preventing or inhibiting hair loss. The peptides of the present invention have the activity of promoting the proliferation of hair follicle dermal papilla cells (HFDPC), activating cell proliferation-related signaling proteins in human dermal papilla cells, activating beta-catenin, improving the expression level of beta-catenin downstream factors, down-regulating the expression level of alopecia protein DKK-1, and increasing the expression of keratin in hair outer root sheath cells (HORSC).

The invention discloses a scar-removing microneedle body and a scar-removing microneedle composite patch, and belongs to the technical field of microneedle patches, the scar-removing microneedle composite patch comprises a patch layer and a microneedle layer, the patch layer comprises a silica gel layer and a substrate layer, and the substrate layer is arranged between the microneedle layer and the silica gel layer; the microneedle layer comprises a plurality of microneedle bodies, each microneedle body comprises an epidermal segment and a dermis segment, and the epidermal segment is composed of a matrix and a first scar removing component; the dermis segment is composed of a matrix and a second scar removing component; the scar-removing microneedle body is of a two-section structural design, two scar-removing components are combined, different scar-removing components of epidermal layer targeting and corium layer targeting are accurately provided to a target skin layer, individual scar-removing components can be concentrated on the target skin layer, and the scar-removing effect is improved. Meanwhile, side effects or interference possibly generated by scar removing components on different skin layers can be avoided, and the precise and efficient compound scar removing effect is achieved.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The present invention relates to a method for preparing an acellular dermal matrix derived from a human body, in which the acellular dermal matrix is washed for the second time with an alcohol-based degreasing solution, thereby not only having excellent safety against cytotoxicity, but also being able to effectively remove residual fat.

The invention discloses Dong'e donkey-hide gelatin active peptide and application thereof. Relates to the technical field of biology. The invention provides an amino acid sequence and application of the Dong'e donkey-hide gelatin active peptide. The amino acid sequence of the CCA active peptide is YYTSASGDEMVSLK, and the CCA active peptide can significantly up-regulate the I-type collagen gene expression, increase the I-type and III-type collagen content and reduce senescence markers. The peptide fragment can be combined with TGFBR2, especially TGFBR1, gene and protein expression of the TGFBR2 can be up-regulated, downstream targets of a TGF-beta-SMAD pathway can be activated, and the MMPs / TIMPs ratio can be reduced. According to the present invention, the TGF beta-SMAD pathway is activated, the MMPs / TIMPs ratio is reduced, the collagen synthesis is promoted, and the excessive decomposition of the collagen is inhibited, such that the I type collagen content and the III type collagen content are increased, and the aging degree of the photoaging dermal fibroblast is reduced;

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention relates to the technical field of cosmetics, in particular to an efficient anti-aging composition and a skin care product containing the composition. The invention discloses an efficient anti-aging composition which is prepared from the following raw materials in parts by mass: 12-15 parts of oil-based concentrated active ingredients, 25-35 parts of base collagen liquid, 15-18 parts of Ectoine, 12-16 parts of TWEEN 20 and 22-25 parts of isosorbide dimethyl ether, and the oil-based concentrated active ingredients are prepared from safflower seed oil, an extract of flowers / leaves / stems of herba lysimachiae, an extract of radix astragali membranaci, ubiquinone and vitamin E; the base collagen liquid comprises XVII type collagen, VII type collagen and IV type collagen. Aiming at the problems that an existing skin care product cannot effectively reduce skin wrinkles, improve skin compactness, reduce skin sagging and prevent skin aging for a long time, the composition disclosed by the invention realizes long-term aging resistance from epidermis to dermis, effectively reduces wrinkles, improves compactness and improves skin sagging.

A multi-technology dermal therapy monolithic handpiece includes a spray spray delivery handpiece having an elongate housing terminating in one or more nozzles for directing a therapeutic spray along an axis toward a subject's skin at a desired spray application distance from the subject's skin; and one or more symbiotic therapeutic handpiece elements terminating in an operative end member having a dermal touch surface for delivering a therapeutic substance to the skin, the therapeutic substance symbiotic with the therapy provided by the therapeutic spray, wherein the operating end member and the touch surface are laterally spaced apart from the axis, and wherein the operating end member and the touch surface are axially offset from the one or more nozzles determined to limit proximity of the one or more nozzles to the skin of the subject to a distance not less than the desired spray application distance.

The invention discloses an anti-inflammatory and anti-aging composition containing a crocus sativus extract and application of the anti-inflammatory and anti-aging composition, and relates to the technical field of cosmetics, the anti-inflammatory and anti-aging composition comprises the crocus sativus extract, a kangaroo claw extract, a dendrobium devonianum stem extract and adenosine; the anti-inflammatory and anti-aging composition can promote human dermal fibroblasts to secrete III-type collagen, can promote human dermal fibroblasts to proliferate and improve the anti-oxidation and anti-inflammatory capacity of the human dermal fibroblasts, has certain anti-inflammatory and anti-aging effects, and can be widely applied to cosmetics with the anti-inflammatory and anti-aging effects.

The invention relates to the technical field of active peptides, and discloses a whitening and anti-wrinkle protein active peptide composition as well as a preparation method and application thereof. The whitening and anti-wrinkle protein active peptide composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of elastin peptide, 11 to 14 parts of fish collagen peptide, 8 to 10 parts of fish maw small-molecule active peptide, 5 to 10 parts of cubilose peptide, 5 to 10 parts of sea cucumber peptide, 2 to 6 parts of Chinese fevervine herb active peptide, 1 to 3 parts of zingiber corianum active peptide and 1 to 5 parts of lycopene. According to the whitening and anti-wrinkle protein active peptide composition and the preparation method and application thereof, small-molecule active peptide in the prepared protein active peptide composition is beneficial to absorption of dermal cells, and meanwhile, through the synergistic effect of multiple active ingredients, the whitening and anti-wrinkle effects are effectively achieved, the skin quality is improved, and aging is delayed.

The application provides a thermoelectric hydrogel composite dressing for promoting repair of diabetic ulcers and loading of targeted modified exosomes and a preparation method, which comprises the following steps: preparing methacrylated hyaluronic acid; preparing polyether F127 diacrylate; co-incubating exosomes with palmitic acid grafted targeting peptides to prepare targeted modified exosomes; mixing the methacrylated hyaluronic acid, the polyether F127 diacrylate, a photosensitizer, the targeted modified exosomes and an electrolyte, and crosslinking under ultraviolet light to form a composite gel. The composite dressing is a novel composite dressing for thermoelectric stimulation and exosome treatment. On one hand, the dressing generates a wireless and passive electric field to simulate the endogenous electric field in the wound, promote directional migration of skin cells, accelerate reepithelialization and dermal formation; on the other hand, the dressing releases exosomes to precisely target blood vessels of a wound lesion, reverse endothelial dysfunction in a high-sugar environment and promote angiogenesis, and effectively solves the problems in repair and regeneration of diabetic ulcer wounds.

The invention provides a skin fibrosis in-vitro model as well as a construction method and application thereof, and relates to the technical field of cell biology. According to the skin fibrosis in-vitro model construction method, a full-thickness skin model with an epidermal layer and a corium layer is constructed by utilizing a cell culture chamber or a cell culture micro-fluidic chip, and the skin fibrosis in-vitro model is obtained by performing induced culture through a chemical reagent, a growth factor or mechanical stress while the corium layer is constructed. According to the construction method, the problem that an existing in-vitro model can only reflect early-stage fibrosis skin characteristics and cannot effectively simulate fibrosis skin formed under the condition of a long disease course is solved. Besides, the corium layer and the epidermal layer in the full-thickness skin structure are not separated, isolated and differentiated through a porous membrane, corium layer fibrosis can generate more direct influence on the epidermis, richer inflammatory factor expression change of the whole model is obvious, and the simulation degree of skin fibrosis diseases is higher.

The invention provides a preparation method of a composite artificial flap with a bionic structure and function induction activity. The preparation method comprises the following steps: S1, preparing an epidermis induction layer; s2, preparing a dermis functional remodeling layer; s3, preparation of a vascularization integration substrate layer; and S4, three-layer compounding and sterilization. Through an epidermis-basilar membrane-dermis three-layer integrated structure, a micron-nano multistage gradient pore and basilar membrane belt adhesion structure of natural skin is accurately simulated, and the problems of epidermis-dermis layering and non-uniform cell infiltration of an existing skin flap are solved.

Systems and methods utilizing RF energy to treat a patient's skin (e.g., dermis and hypodermis) or other target tissue including at a depth below a tissue surface (e.g., skin surface, mucosal surfaces of the vagina or esophagus) are provided herein. In various aspects, the methods and systems described herein can provide a RF-based treatment in which the deposition of RF energy can be selectively controlled to help ensure heating uniformity during one or more of body sculpting treatment (lipolysis), skin tightening treatment (laxity improvement), cellulite treatment, vaginal laxity or rejuvenation treatment, urinary incontinence treatment, fecal incontinence treatment, all by way of non-limiting examples. In various aspects, the systems can comprise one or more sources of RF energy (e.g., a RF generator), a treatment applicator comprising one or more electrode arrays configured to be disposed in contact with a tissue surface, and a return electrode (e.g., a neutral pad) to the tissue surface.

The invention relates to the field of biological dressings, and discloses an artificial dermis dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: adding water into a container, and freezing until the water is frozen; then adding a collagen A solution, freezing and standing; continuing to add collagen B liquid, freezing and standing; continuing to add collagen A liquid, freezing and then freeze-drying; and cross-linking in a glutaraldehyde solution, and cleaning to obtain the artificial dermis dressing. The dressing provided by the invention can provide a good stent effect and promote cell migration and proliferation, and the healing rate is close to 100% after wound making. In the wound healing process, the dressing can inhibit collagen deposition, relieve inflammatory response, inhibit abnormal hyperplasia of blood vessels, inhibit excessive proliferation of fibroblasts and excessive formation of granulation tissues, and effectively prevent scar formation. The dressing can also effectively inhibit abnormal vascular hyperplasia in a wound area, and fibrosis and scar hyperplasia caused by excessive vascular hyperplasia are prevented.

The invention provides a beautifying composition and application thereof in whitening, anti-inflammatory and anti-aging products, and belongs to the technical field of cosmetics. The beautifying composition is prepared from collagen peptide, sodium hyaluronate and ergothioneine, wherein the mass concentration ratio of the collagen peptide to the sodium hyaluronate to the ergothioneine is (75 to 85) to (3.5 to 10) to (0.4 to 3.5); the beautifying composition shows remarkable advantages in the aspects of collagen regeneration and skin improvement, the collagen content can be remarkably increased, and multi-effect collagen regeneration promotion is achieved by inhibiting collagen degrading enzyme MMP-1 and up-regulating TGF-beta1; the content of AQP3 and HA can be increased, and water locking and moisturizing of the skin are enhanced; by activating an Nrf2 pathway, the SOD2 level is improved, IL-6 and IL-1alpha are inhibited, and the antioxidant, anti-inflammatory and whitening effects are achieved; the thickness of an epidermal layer and the density of dermal fibroblasts can be remarkably improved, wrinkle resistance and repairing are effectively achieved, and the skin structure is improved.

The present invention features methods of manipulating subdermal fat in a treatment area. Such methods include inserting a needle (e.g., a micro-coring needle) through the dermis to the subdermal fat layer in order to excise a portion of tissue from the treatment area. These insertions result in a portion of the dermis and a portion of the subdermal fat to enter into the needle. Removal of the needle results in excision of the portion of dermis and subdermal fat that entered into the needle.

The invention relates to a multifunctional intelligent mobile infusion apparatus and a control method thereof, the multifunctional intelligent mobile infusion apparatus comprises: a real-time monitoring system, the real-time monitoring system comprises a pulse wave sensor, a blood oxygen sensor and a heart rate sensor, and the real-time monitoring system is used for collecting vital sign data of a patient in real time; the accurate infusion control pump is used for accurately controlling the infusion amount; the liquid heating module adjusts the temperature of infusion liquid according to requirements of a patient; the invention discloses a nanoneedle dermis puncture device and a control system thereof, which are used for ensuring painless infusion. The spectrum blood vessel recognition system is used for automatically recognizing blood vessel positions; the wireless communication module is used for supporting remote data transmission and equipment control, so that medical staff can monitor the infusion condition of the patient at different places; and the user interaction interface comprises a touch screen and voice recognition control and is used for performing equipment operation and state display, so that a user can operate and acquire information conveniently. The functions of high-precision infusion control, real-time vital sign monitoring, remote control, painless infusion, intelligent interaction and the like are achieved.

Radio-frequency (RE) microneedling skin resurfacing systems are described including an electrode with an array of partially insulated needles, where the needles' exposed tips conduct bipolar RF electric current into a patient's skin, thus enhancing collagen production that stretches the skin. The electrode is carried by an application handle, which connects to an RF current generator. The applied treatment cycles utilize vacuum to pull the skin against the needles and dielectric coolant to cool the epidermis. The needles' array penetrates the skin to a pre-determined depth inside the dermis layer, followed by a sequential delivery of RF bipolar current pulses. The treatment cycle is controlled by an algorithm, designed to raise the dermis layer temperature for a certain duration. The treatment cycle and algorithm rely on dynamic impedance measurements and temperature measurements by thermistors to assure optimized controlled heating of the dermis layer to a target temperature between 45 and 69°C.

The invention relates to application of a fish maw and colla corii asini composition in preparation of anti-aging products, and belongs to the technical field of health-care products. The mass ratio of the fish maw to the colla corii asini in the fish maw and colla corii asini composition is (1-2): 1, and the colla corii asini is Dongshan colla corii asini powder. The fish maw and colla corii asini composition has the advantages that the anti-oxidation level, the collagen metabolism level and the cell function level are mutually cooperated, the anti-oxidation capability is obviously improved, the dermis layer is delayed to be thinned, the skin elasticity is effectively improved, and the anti-aging effect is achieved.

A use of liquiritin in the preparation of a drug for treating atopic dermatitis. Animal experiments show that liquiritin can remarkably lessen the skin lesion severity of an atopic dermatitis mouse model, reduce the ear thickness and the epidermal thickness, and reduce the infiltration degree of dermal lymphocytes, thereby demonstrating that liquiritin has a significant therapeutic effect on atopic dermatitis, has no toxic and side effects on the liver, the spleen and the kidney, and has good clinical application prospects and enormous social benefits, and expanding a new application field for a conventional leguminous plant liquorice extract, i.e., liquiritin.

The invention provides a mobile terminal skin parameter inversion method based on spectral information, and the method comprises the following steps: constructing an optical model according to the characteristics of a skin layered structure and a spectral response mechanism of three skin parameters: melanin content, hemoglobin concentration and dermis thickness, and building a skin parameter and reflection spectrum database based on the model; based on the database, establishing a skin multi-parameter inversion MLP neural network structure adaptive to the collection characteristics of a mobile phone end sensor, and screening an optimal four-waveband channel through multi-waveband combination traversal; the mobile phone spectrum sensor is subjected to wave band optimization, data acquisition and skin parameter inversion. According to the method, through efficient decoupling of a nonlinear coupling relation among multiple skin parameters by waveband optimization and an MLP model, and targeted design of four optimal visible light narrow-band channels, synchronous quantitative detection of melanin, hemoglobin and dermis thickness is realized, core physiological parameters from epidermis to dermis superficial layer are covered, information coverage is comprehensive, and specificity is high.

The present invention relates to an injectable dermal filler formulation for providing an improved and homogeneous volumizing and filling effect. In particular, the formulation comprises a combination of hyaluronic acid and poly(orthoester) and may be used for cosmetic purposes.