The invention discloses smearing type intradermal injection capable of directly reaching the dermis layer and a preparation method thereof. The smearing type intradermal injection comprises, by weight, 10-30 parts of hyaluronic acid and 10-30 parts of collagen. The smearing type intradermal injection has the advantages that the smearing type intradermal injection is high in activity, appropriate in component and reasonable in combination, the smearing type intradermal injection can permeate the epidermis to reach the dermis layer when the smearing type intradermal injection is applied to the face or smeared to the epidermis of the skin, the hyaluronic acid and collagen lost due to aging are injected to allow the skin to absorb and store moisture with the weight being 1000 times of the weight of the skin, the regenerative function of cells is awakened, the withered and fractured cells of the skin are repaired again, the compact, smooth and elastic skin can be achieved again in a short time, effects of removing yellowness and whitening and resisting wrinkles and tightening the skin are achieved, the skin can restore elasticity, be transparent, bright and compact and form a moisture shield, different mechanisms are coordinated to bring an anti-ultraviolet effect into play, ultraviolet permeation can be reduced, the skin damage caused by the ultraviolet can be repaired, dual-protection and a good effect are achieved, and the smearing type intradermal injection has a quite good anti-allergic function.