47 results about "Tissue skin" patented technology

The invention discloses a skin targeted therapy laser control method and system based on a dual-wavelength synergistic effect, and the method comprises the steps: collecting the real-time morphological characteristics and pigment distribution data of skin tissues through a multispectral imaging unit, and recognizing the optical characteristic parameters of a target treatment region; generating a dual-wavelength combination scheme through a dynamic wavelength selection algorithm based on the optical characteristic parameters and the tissue structure characteristics of the target treatment area; according to a dual-wavelength combination scheme, generating a synergistic laser sequence with a specific pulse interval and energy ratio; the synergistic laser sequence is converted into a driving signal, and a dual-wavelength laser is controlled to output composite laser; and laser output parameters are dynamically adjusted through real-time thermal diffusion monitoring and an optical feedback mechanism. By utilizing the embodiment of the invention, accurate and selective heat dissipation of skin target tissues can be realized, surrounding normal tissues are effectively protected, the treatment safety and effectiveness are improved, and side effects are reduced.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention discloses a preparation method and application of cubilose peptide. The amino acid sequence of the cubilose peptide is KPAPPKPEPK. The cubilose peptide prepared by the invention has strong ultraviolet damage resistance, and can improve the cell survival rate, improve the antioxidant enzyme level in serum, scavenge free radicals and reduce the inflammatory factor level in skin tissues. When the cubilose peptide is used for developing anti-ultraviolet damage products, the efficacy of cubilose can be deeply excavated, and the quality and economic value of cubilose products are improved.

The invention relates to the technical field of functional nutritious food, in particular to multi-layer skin nutrition powder with a whole-spectrum skin growth and repair promoting effect and a preparation method and application thereof, the core of the multi-layer skin nutrition powder is composed of a collagen peptide system (60-62%), a proteoglycan system (20-22%) and the like, collagen peptide is compounded with three sources of cattle, fish and chicken, and the multi-layer skin nutrition powder has the advantages that the multi-layer skin nutrition powder is prepared; according to the present invention, with the combination of the following components, the skin structure and activity are jointly promoted by using the synergistic effect of the functional polypeptide compound (2.5-3.5%) and the plant active component (3.5-4.5%) rich in asiaticoside and procyanidine, and the nutrition enhancers (4-5%) such as L-ascorbic acid and amino acid chelated zinc and the food-grade auxiliary materials (8-10%) such as microcrystalline cellulose are integrated in the formula, such that the various systems achieve the synergistic effect so as to achieve the effect of nourishing the skin; through the synergistic compatibility of the three-source collagen peptide, the comprehensive supplement of various types of collagen in skin tissues is realized.

The application belongs to the technical field of biomedical optical imaging, and more particularly relates to a biosafe skin optical clearing agent and method based on a lysine synergistic system. The biosafe skin optical clearing agent provided in the application contains lysine or a salt thereof as an active ingredient, can effectively reduce the light scattering of skin tissue, and thus realizes the optical clearing of skin, and meanwhile, the morphology of the skin tissue is well maintained during the treatment. The skin optical clearing agent has the advantages of simple active ingredient, high biosafety, remarkable skin optical clearing effect, convenient preparation, simple application operation and the like, and is suitable for the optical clearing treatment of ex vivo skin and in vivo skin.

This application belongs to the field of image processing and relates to a skin extraction method and system. The method includes: performing nonlinear regression fitting on the prior anatomical features of skin tissue in historical oral images to obtain a skin cutting threshold; acquiring a CBCT image of the oral cavity to be processed; extracting boundary features from the cross-sectional image layers of the CBCT image to be processed based on the skin cutting threshold to obtain a dynamic candidate masking region feature map; applying inter-layer geometric consistency constraints to the dynamic candidate masking region feature map to generate a target skin masking region; extracting the original pixel values ​​within the target skin masking region and suppressing their background values; performing isosurface evolution processing on the suppressed original pixel values ​​to generate an isosurface grid; and performing patch validity screening on the isosurface grid to generate the skin extraction result. This application can reduce the interference of irrelevant data on the extraction result during the skin extraction process and ensure the quality of skin tissue extraction as much as possible.

The invention discloses an infrared photothermal collaborative beauty / medical beauty roller capable of replacing microneedles and a using method thereof, and belongs to the technical field of beauty / medical beauty instruments. The roller comprises a handle, a roller body and a replaceable microneedle assembly. The roller main body is provided with a hollow frame, and an infrared light source is arranged in the hollow frame; the replaceable microneedle assembly comprises a light-transmitting microneedle patch and a mounting seat, and is detachably mounted on the roller main body; a light-transmitting area is arranged on a frame of the roller main body, and the microneedle patch is made of a light-transmitting high-molecular polymer, so that infrared light can penetrate through the light-transmitting area and the microneedle patch to irradiate the skin. Infrared light is converted into heat energy in skin tissue, traditional contact type electric heating is replaced, and the risk of scalding is fundamentally avoided; meanwhile, the high-light-transmittance microneedle structure is combined with the roller hollowed-out design, efficient cooperation of microneedle transdermal, infrared phototherapy and endothermic heat is achieved, and the device is suitable for skin care. The microneedle assembly can be replaced and is safe and sanitary.

The invention discloses a biological index data processing method and device, a storage medium and computer equipment, relates to the technical field of information processing, and mainly aims to improve the determination efficiency and the determination accuracy of radiation response characteristics of a biological index data set. The method comprises the following steps: acquiring a biological index data set of a skin tissue sample in a non-radiation exposure state; determining the degree of influence of each piece of biological index data in the biological index data set on radiation response characteristic prediction, and screening out core biological index data related to radiation response characteristics from the biological index data set based on the degree of influence; and analyzing the core biological index data by using a preset radiation response characteristic prediction model, and determining radiation response characteristics of the biological index data set based on an analysis result. The method is suitable for processing scenes of the biological index data.

This invention discloses a mitochondrial-targeted nanomaterial for senescent cells and its application in anti-aging. The material is a nucleic acid aptamer-tetrahedral DNA, mainly assembled from four single-stranded DNA sequences, SEQ ID Nos. 1-4. Preparation involves first hybridizing the four single-stranded DNA sequences, then concentrating them to obtain aptamer-modified tetrahedra, which are then coupled with tannic acid (TA) via a Mann reaction in the presence of formaldehyde to obtain the Apt-tFNAs-TA complex. Finally, it is incubated with excess catechins to obtain the final delivery material, Apt-tFNAs@catechin-TA. This invention's material exhibits good biocompatibility and skin tissue compatibility, significantly improving uptake efficiency and mitochondrial localization ability in senescent cells. It can effectively deliver polyphenolic compounds such as catechins and protocatechuic acid, improving mitochondrial function and addressing the technical problems of low delivery efficiency of active ingredients and insufficient targeted enrichment in senescent cells.

The invention discloses an ultrasonic treatment auxiliary film and a use method thereof, relates to the technical field of ultrasonic treatment, and discloses the ultrasonic treatment auxiliary film which is a film with non-uniform thickness, and the thickness of the ultrasonic treatment auxiliary film is complementary with that of target skin tissue. Compared with the prior art that the treatment depth of each treatment head of an ultrasonic treatment instrument is fixed, so that the specific layer of the human tissue cannot be accurately treated, the thickness of the ultrasonic treatment auxiliary film is not uniform and is complementary with the thickness of the target skin tissue, so that the depth of the skin tissue of the user can be compensated, and the treatment accuracy is improved. Therefore, the ultrasonic therapeutic apparatus can accurately treat the specific layer of the human tissue based on the compensated skin depth, and the technical problem that the specific layer of the human tissue cannot be accurately treated due to the fact that the treatment depth of the treatment head of the ultrasonic therapeutic apparatus is fixed and the depths of the human tissue are different in the prior art is solved.

According to the isolated culture identification and key marker detection method for the down producing goat hair follicle stem cells, trypsin and EDTA are used for jointly digesting skin tissue, and high-activity cells are efficiently obtained; a culture medium containing various growth factors is adopted for culture, and cell proliferation and stemness maintenance are promoted. And identifying and integrating morphological observation, growth curve determination and clone formation experiment to accurately judge the characteristics of the cells. Immunofluorescent staining and real-time fluorescent quantitative PCR are applied to key marker detection, and expression of markers such as CK15, CD34 and beta1 integrin is detected with high sensitivity. The method is high in separation efficiency, accurate in identification and sensitive in marker detection, can provide sufficient high-quality cells for the research on the hair follicle stem cells of the cashmere goat, assists in deeply understanding the biological characteristics and the regulation mechanism of the cells, and is of great significance for improving the yield and quality of cashmere and promoting the development of related biotechnologies.

The application discloses a tripeptide with the effects of promoting HaCaT cell migration and barrier repair and application of the tripeptide, and the amino acid sequence of the tripeptide is glycine-methionine-hydroxyproline. The tripeptide has the effects of promoting HaCaT cell migration and barrier repair, and can be applied to the preparation of cosmetics with the effects of skin tissue repair and / or skin barrier repair.

The application discloses a jaundice meter based on programmable digital LED microarray, which comprises three parts of digital LED microarray, photoelectric conversion module and main control chip. The measurement comprises the following steps: the light emitting units in the digital LED microarray are controlled by a preset program to emit light in a combined form in sequence, the light beams are collected by a light trapping window after being reflected and absorbed by skin tissue, and are transmitted to the photoelectric conversion module through an optical fiber to be converted into digital electrical signals; the measurement signals obtained each time contain multi-dimensional information such as epidermis thickness, pigment distribution and blood vessel depth; and the accurate transcutaneous jaundice value is obtained by analyzing and calculating the comprehensive differentiated measurement results. The application can solve the inaccurate measurement problem caused by system errors such as environmental light brightness, interfering molecule distribution and individual skin differentiation based on the time and space controllable excitation mode.

The invention discloses a soft tissue filling material, an injectable soft tissue filling agent and application of the injectable soft tissue filling agent, and belongs to the technical field of biomedical materials. The soft tissue filling material mainly comprises polymers of formulas i to vii. The soft tissue filling material disclosed by the invention can generate conformational change under the action of active oxygen, can effectively enhance the filling effect through reconstruction of a multi-layer structure, can be retained in skin tissues for a long time and stably maintain the volume shape and position, and has an excellent instant and lasting filling effect; meanwhile, the long-acting anti-inflammatory and anti-oxidation functions, collagen generation promoting, water locking and moisturizing functions and the like are achieved, and the composition can be used for wound healing, tissue filling and repairing, skin moisturizing and water locking, anti-aging and anti-oxidation treatment, skin barrier repairing and the like.

The invention discloses a skin tumor three-dimensional electrical impedance tomography method and device and a storage medium. The method comprises the following steps: acquiring a skin surface boundary voltage signal by using a planar electrode, and generating a space virtual electrode equivalent voltage through nonlinear mapping from a planar electrode domain to a space virtual electrode domain; mapping the voltage into a two-dimensional voltage feature map through bilinear interpolation, inputting the feature as prior information into a three-dimensional electrical impedance imaging reconstruction network, and performing depth matching inversion on the body area conductivity distribution based on a sliding window SwinUNet network to obtain a three-dimensional conductivity image; constructing a skin tissue finite element model, and generating a simulation data set; and training nonlinear mapping by adopting the simulation data set and verifying the depth matching inversion performance. The method can still keep stable reconstruction quality under uncertain conditions such as poor electrode contact, channel loss and skin structure change, has high robustness and adaptability, and has good clinical application value.

Disclosed herein is a system and method for determining an optimal clinical outcome, including receiving an image of skin tissue of a patient from an image capture device; analyzing one or more images of skin tissue; and determining skin characteristics and physiological factors of the skin tissue based on the analyzed one or more images. The system and method further analyzes the determined skin characteristics and physiological factors of the skin tissue; determining treatment parameters based on the analyzed determined skin characteristics and physiological factors; generating a skin response predictor image predicting a result of the processing based on the one or more images of the skin tissue and a combination of the processing parameters; scoring the skin response predictor images based on security and predetermined efficacy criteria; and displaying one or more processing parameters based on the scores of the skin response predictor images.

The invention discloses a drug-loaded microneedle patch loaded with a neutrophile granulocyte membrane, the microneedle patch comprises a sheet-shaped backing and soluble microneedle bodies arranged on the sheet-shaped backing, the microneedle bodies comprise the neutrophile granulocyte membrane and drugs, and the drugs comprise antibacterial drugs or / and antibiotics. The invention further discloses a preparation method of the drug-loaded microneedle patch loaded with the neutrophile granulocyte membrane and application of the drug-loaded microneedle patch loaded with the neutrophile granulocyte membrane to preparation of drugs for treating subcutaneous bacterial infection. The microneedle patch can effectively kill drug-resistant bacteria, remove biological membranes, activate immune response, reduce the bacterial load of bacterial infected skin and promote skin tissue healing, and has important application prospects in the field of drug-resistant bacteria infection treatment.

The application discloses a preparation method of bird's nest peptide and application of the bird's nest peptide. The amino acid sequence of the bird's nest peptide is KPAPPKPEPK. The bird's nest peptide prepared by the application has strong anti-ultraviolet damage ability, can improve cell survival rate, improve the level of antioxidant enzymes in serum, remove free radicals and reduce the level of inflammatory factors in skin tissue. The bird's nest peptide is used for development of anti-ultraviolet damage products, so that the efficacy of the bird's nest can be deeply explored, and the quality and economic value of the bird's nest products are improved.

The invention discloses lactobacillus reuteri CCFM1502 capable of up-regulating skin ceramide synthesis and reducing skin inflammation and a metagen of the lactobacillus reuteri CCFM1502, and belongs to the technical field of microorganisms and medicines. The lactobacillus reuteri CCFM1502 provided by the invention can remarkably promote synthesis of ceramide when being externally used and orally taken, and can reduce skin inflammation response of mice after being orally taken. Specifically, the strain lysate and the fermentation supernatant both can promote HaCaT cells to secrete ceramide; the total amount of ceramide in skin can be up-regulated by oral administration of viable bacteria and metabiotics of the lactobacillus reuteri, apparent indexes of the skin can be regulated and controlled, inflammatory reactions in vivo and tissues can be reduced, and particularly, the effect of fermentation supernate of the lactobacillus reuteri is optimal. And the thallus components and viable bacteria have obvious effects in promoting the expression of anti-inflammatory factors in skin tissues. Therefore, the lactobacillus reuteri has a huge application prospect in preparation of a product for promoting synthesis of the natural active ingredient ceramide of the skin.

The invention provides a chiral manganese dioxide nanoparticle and a preparation method and application thereof. The chiral manganese dioxide nanoparticle having good biocompatibility is prepared by taking D-gluconic acid as a ligand. The chiral manganese dioxide nanoparticle of the present invention has a strong circular dichroism signal in a visible light region, so that interference of signals in a small molecule ultraviolet region in an organism can be avoided; and by means of the redox reaction with ROS, change in the circular dichroism signal intensity of the chiral manganese dioxide nanoparticle presents a good linear relationship with the concentration of ROS, so that the quantitative detection of ROS is achieved. The chiral manganese dioxide nanoparticle prepared in the present invention can effectively remove ROS in aged skin, increase dermis thickness and collagen proportion, and reduce the oxidative stress level in skin tissue, and is beneficial to delaying skin aging.

The invention relates to the technical field of biomedical materials, in particular to a collagen preparation method which comprises the following steps: performing non-collagen impurity removal and degreasing treatment on animal skin tissues to obtain pretreated skin tissues; performing enzymolysis and salting-out on the pretreated skin tissue to obtain a collagen solution; diluting the collagen solution through an acid extraction buffer solution to obtain a diluted collagen solution; adding a salt solution at the temperature of 0-4 DEG C into the diluted collagen solution, and stirring to obtain a collagen solution to be purified; and purifying the collagen solution to be purified through the resin filler to obtain the collagen. Under the conditions of no organic solvent and no denaturing agent, the collagen can be efficiently purified on the premise of keeping the natural triple-helix structure of the collagen and ensuring the biological activity by combining the low-temperature low-ionic-strength pretreatment with the resin purification technology, and the long-standing technical bottleneck that the purification efficiency and the biological activity are difficult to consider at the same time in the industry is solved.

The invention discloses a noninvasive AGEs concentration monitoring method and system, a storage medium and a watch. The method comprises the steps that excitation light sources with different wavelengths are adopted to excite fluorescence in skin tissue; obtaining the light intensity of autofluorescence under different wavelengths; the AGEs concentration is obtained according to discrete three-dimensional fluorescence spectrums established according to different excitation wavelengths. By means of the non-invasive characteristic, the comfort level and convenience of monitoring are remarkably improved, and a user can continuously monitor the health condition without interference in daily activities. The combination of an ultra-sensitive optical sensor and an intelligent algorithm not only ensures the accuracy and real-time performance of data, but also provides a reliable basis for early diagnosis and timely intervention.

The invention relates to a method for analyzing the transdermal permeation content and distribution of cosmetic active ingredients, which comprises the following steps: smearing the cosmetic containing the active ingredients on artificial skin for permeation treatment, and freezing and slicing to obtain a sample; the method comprises the following steps: acquiring data by adopting a desorption electrospray ionization mass spectrometry imaging technology, and extracting a peak table containing position, mass-to-charge ratio and intensity information; performing normalization processing on the target object intensity of each pixel point by taking the mass locking internal standard object as a reference to generate a normalized mass spectrum imaging graph; identifying a skin tissue area by using the endogenous characteristic substance signal distribution of the skin and adopting an image segmentation algorithm; the average normalized intensity of the target object in the area is calculated to quantify the permeability content difference, and the distribution uniformity is evaluated through a variable coefficient. Compared with the prior art, the method can accurately quantify the content change of the target object under different conditions, and supports the relative content analysis and distribution uniformity analysis of the target object in the data acquired within the day and during the day in a long range.

A collar device is described herein comprising a housing including an emitter and a detector, wherein a base of the housing exposes the emitter and the detector. A spacer component comprising a plurality of optical pathways, wherein the plurality of optical pathways comprises a first optical pathway and a second optical pathway. The spacer component is secured to the base of the housing, wherein the first optical pathway is positioned over an emitter, wherein the second optical pathway is positioned over a detector. The emitter is configured to project light through the first optical pathway toward skin tissue of an animal. The detector is configured to detect portions of the light reflected by the skin tissue back through the second optical pathway. One or more applications running on at least one processor are configured to receive information of the reflected light and use the information to determine a biological metric.

Cosmetic composition for skin cell longevity The present invention relates to a composition comprising a combination of at least two cosmetic active agents, said combination of active agents preventing epigenetic modifications, preventing or reducing the loss of proteostasis, preventing or reducing micro- inflammatory processes, and preventing or reducing mitochondrial dysfunction and / or stimulating mitochondrial energy metabolism in skin cells The invention also relates to the cosmetic use of such a composition for increasing the longevity of skin cells and skin tissues, preventing epigenetic modifications, preventing or reducing the loss of proteostasis, preventing or reducing micro-inflammatory processes, and preventing or reducing mitochondrial dysfunction and / or stimulating mitochondrial energy metabolism, in skin cells. Finally, the invention relates to a nontherapeutic cosmetic process for caring for keratin materials comprising the topical application of such a composition.

The invention relates to the technical field of skin tissue image recognition, in particular to a skin tissue image recognition method based on polarization imaging and a related device. The method comprises the following steps: firstly, acquiring DoP and AoP images of an undyed skin tissue slice; then, extracting four polarization characteristic parameters, including a DoP histogram peak value, a DoP histogram slope, a high-polarization region average DoP and an AoP texture anisotropy index, of the DoP image and the AoP image; and finally, performing skin tissue image recognition according to the four polarization characteristic parameters to obtain a skin tissue image recognition result. According to the method, undyed skin tissue slices are adopted, and a dyeing step is not needed, so that the detection period is shortened; meanwhile, the mode that an existing identification means based on polarization imaging needs to depend on a Mueller matrix is broken through, the detection process is greatly simplified, and the problems that a traditional polarization image identification process is complex, cost is high and accuracy is poor are solved.

The invention belongs to the technical field of medical ultrasonic imaging agents, and particularly relates to medical ultrasonic hydrogel and a preparation method thereof. According to the application, the tannic acid is subjected to alkylation modification, so that the lipid solubility of the tannic acid is enhanced, the tannic acid can permeate cell membranes of gram-positive bacteria and gram-negative bacteria in a short time and play a bactericidal role, and the sterilization rate of staphylococcus aureus and escherichia coli within 15 minutes can be greater than 95%. Meanwhile, the alkylated modified tannic acid is beneficial to reducing the sound attenuation of the hydrogel and the adhesion strength to the skin tissue surface, so that the hydrogel can be conveniently stripped from the skin surface and the ultrasonic probe can smoothly slide, and the poor user experience caused by pulling the skin is avoided.

The invention relates to the technical field of biomedical regenerated materials, in particular to a silk fibroin-polyester composite regenerated material and a preparation method and application thereof. The composite regenerated material disclosed by the invention is prepared from aliphatic polyester and silk fibroin in a blending or copolymerization manner; the mass ratio of the aliphatic polyester to the silk fibroin is (1: 20)-(20: 1). According to the invention, the silk fibroin is introduced into the aliphatic polyester, so that the hydrophobicity of the aliphatic polyester can be improved, and after the silk fibroin is used for injecting the filler into the skin, the elasticity and moisturizing capacity of the skin can be improved, and the growth of new collagen in the skin can be promoted. Through the synergistic effect of the two components, the composite regeneration material has a controllable degradation period, good biocompatibility, biological absorbability and proper mechanical properties, can promote cell adhesion and migration in skin and tissues, can be prepared into microspheres or temperature-sensitive gel and other dosage forms with specific forms, and has a good application prospect. The efficient application of the composite regenerated material can be realized.

The invention discloses in-vitro tissue engineering skin automatic cleaning equipment, and belongs to the technical field of cleaning equipment, the in-vitro tissue engineering skin automatic cleaning equipment comprises a cleaning tank, an ultrasonic cleaning module is arranged in the cleaning tank, the rear side of the cleaning tank is communicated with a liquid discharge pipe, a partition plate is mounted in an inner cavity of the cleaning tank, and the ultrasonic cleaning module is located at the top of the partition plate. According to the tissue engineering skin cleaning and rubbing device, the cleaning and rubbing effect on tissue engineering skin is improved through mutual circumferential movement of the pretreatment rollers on the two sides, the pretreated tissue skin falling into the surface of the conveying roller belt can be conveyed to the compaction mechanism on the rear side, and ultrasonic cleaning is conducted through ultrasonic waves emitted by the ultrasonic cleaning module in the cleaning tank in the compaction process; according to the in-vitro tissue engineering skin cleaning device, residual impurities of tissue skin are cleaned into a medium in the cleaning tank through ultrasonic waves, automatic cleaning of the in-vitro tissue engineering skin is achieved through pretreatment and compaction superposition cleaning, and cleaned materials can be conveyed to the tail section of the cleaning tank through a transmission roller to be conveniently taken and used.

The invention relates to a preparation method of a porous hydrogel microneedle based on an ice template method and application of the porous hydrogel microneedle in interstitial fluid extraction, and belongs to the technical field of microneedles. The micro-needle is prepared by adopting an ice template method: preparing a micro-needle preparation solution and injecting the micro-needle preparation solution into a micro-needle mold; placing in a controllable low-temperature environment with a vertical temperature gradient difference, and guiding water molecules to directionally crystallize to form a directional ice crystal framework serving as a pore-foaming agent; and after the frozen structure is stable, curing the polymer network through ultraviolet crosslinking, and freeze-drying to sublimate ice crystals, thereby forming the hydrogel microneedle with high porosity and a directional through pore structure. The method is easy and convenient to operate, free of organic solvent or high-temperature treatment, environmentally friendly and suitable for industrial large-scale production. The prepared porous hydrogel microneedle can synergistically utilize capillary action and osmotic pressure generated by swelling of hydrogel to realize efficient and rapid extraction of interstitial fluid, so that real-time, rapid and accurate detection of biomarkers in skin tissues is promoted, and the porous hydrogel microneedle has a wide application prospect.