45 results about "Virus load" patented technology

The invention belongs to the technical field of disease drugs, and particularly relates to application of a recombinant attenuated coxsackie B3 virus loaded with an anti-inflammatory factor in preparation of drugs for treating traumatic craniocerebral injury. The virus can regulate and control a peripheral immune phenotype capable of inhibiting inflammation, after severe craniocerebral injury of a mouse, a host immune system is remodeled through single intraperitoneal injection treatment, a treatment effect is achieved in improving an immune microenvironment in the acute stage of TBI, the neuroprotective effect is observed in the early stage and the late stage of TBI, and safety is achieved.

The invention relates to the technical field of inspection process automation, in particular to an automatic process control system and method for a clinical laboratory, and the system comprises a pedigree depth analysis module, a sound spectrum precise positioning module, a virus load calculation module, a spectrum cell recognition module, a cell electrical performance analysis module, a sample comprehensive decision module and an intelligent sample processing module. According to the method, multiple advanced technologies such as image spectrum analysis, sound spectrum analysis, time-frequency domain analysis, spectrum technology and cell electrical impedance analysis are combined, so that high-precision analysis and deep learning processing of the sample are realized, and the accuracy and efficiency of sample analysis are improved; the system especially has obvious advantages in the aspects of virus load calculation, cell type identification, sample risk assessment and the like, the application of the intelligent sample processing module optimizes the sample processing flow, improves the working efficiency and the processing speed, and greatly improves the automation degree and the intelligent level of the work of the clinical laboratory.

The invention discloses a primer, a kit and a detection method for detecting tomato brown wrinkled fruit virus based on a digital PCR technology, and relates to the technical field of plant virus biological detection.The primer comprises ToBRFV-CqF and ToBRFV-CqR, the nucleotide sequence of the ToBRFV-CqF is shown as SEQ ID NO: 1, and the nucleotide sequence of the ToBRFV-CqR is shown as SEQ ID NO: 2. The method disclosed by the invention has extremely high sensitivity, specificity and repeatability, can be used for accurately detecting a sample with extremely low virus loading quantity, and can be directly used for quantitatively monitoring the tomato brown wrinkled fruit virus in the field.

The invention belongs to the technical field of pseudorabies virus prevention and control, and discloses application of BAY87-2243 in preparation of a drug for inhibiting pseudorabies virus proliferation, and the application is as follows: firstly, PK-15 is infected with a PRV-GD-WH strain, total RNA of cells is taken, and cDNA is obtained through reverse transcription; the change of the transcriptional level of a glycolysis key gene in a cell is detected through fluorescent quantitative PCR (Polymerase Chain Reaction) of an SYBR Green method. BAY87 is further used for acting on the HIF-1alpha, the regulation and control effect of the HIF-1alpha on glycolysis is verified, and finally, the virus load in cells is detected through SYBR Green fluorescent quantitative PCR (Polymerase Chain Reaction) and the inhibition effect of BAY87-2243 on PRV proliferation is determined through TCID50. Meanwhile, the invention also provides a pseudorabies virus inhibition drug.

This article provides a combination of compounds that can inhibit influenza virus replication, reduce influenza virus load, and / or treat influenza.

The invention discloses an HIV-1 virus load detection performance evaluation method and system. For each pair of data points (xi, yi) in the data set D, the slope a and the intercept b of a straight line between all possible two points are calculated; the estimated value of the slope a is the median of all the slopes. The estimation of the intercept b is obtained by calculating the residual error of each data point and finding the median of the residual errors; the invention relates to the technical field of virus load detection. According to the invention, through multi-dimensional analysis including correlation, consistency, classification accuracy, chi-square test, Bland-Altman consistency analysis, Kappa coefficient and the like, a comprehensive performance evaluation framework is provided for the virus load detection kit. The comprehensive evaluation is helpful for ensuring that the kit can provide reliable results in various application scenarios. Through strict experimental design and data analysis, possible problems of the kit can be found and corrected in time, so that the detection accuracy and reliability of the kit are improved.

The invention discloses a rapid breeding method for trichosanthes kirilowii maxim seedling detoxification, belongs to the technical field of trichosanthes kirilowii maxim seedling breeding, aims at solving the problems that traditional breeding is prone to carrying viruses and low in efficiency, and provides high-quality trichosanthes kirilowii maxim seedlings which are low in virus load, do not affect yield and quality and are stable in heredity. The method comprises the following steps: selecting an annual trichosanthes kirilowii maternal plant, screening a healthy maternal plant by using a trichosanthes kirilowii maternal plant healthy screening model, and taking a tender stem section for pretreatment; sterilizing the pretreated tender stem segments, stripping stem tips, and inoculating a starting culture medium to obtain sterile buds; cutting the sterile buds, transferring to a proliferation culture medium, and propagating to obtain bud clusters; screening qualified buds through a trichosanthes kirilowii maxim sterile bud screening model after molecular virus detection on the bud clumps; after qualified buds are subjected to rooting culture, acclimatization seedlings are transplanted into a sterilization matrix, and virus-free trichosanthes kirilowii maxim seedlings are obtained through cultivation in a greenhouse. According to the method, the detoxification effect is controllable, the breeding efficiency is high, the seedling quality is stable, and the obtained detoxified trichosanthes kirilowii maxim seedlings meet the agricultural production requirements, have stable production applicability and are suitable for large-scale production of the trichosanthes kirilowii maxim seedlings.

A static building structure may include an athletic facility. The facility may include a locker room area having disposed therein one or more lockers including ultraviolet lamps. In addition, the facility may include one or more high flow ultra high efficiency particulate air (HEPA) UV-C purifiers. Further, the athletic facility may have a shower area disposed adjacent the locker room area and including one or more far-UV-C lamps.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The application discloses a single-tube quantitative detection HBV viral load real-time fluorescent quantitative PCR kit and belongs to the technical field of molecular biological detection. The kit comprises a primer probe combination for single-tube quantitative detection HBV viral load real-time fluorescent quantitative PCR, wherein the primer probe combination comprises a primer pair and a probe for detecting a target gene, and a probe IC-P1 and a probe IC-P2 for detecting an internal standard. The kit can cover various genotypes of HBV viruses, and the minimum detectable viral load is 2.5 IU / mL, the detection sensitivity is high, the repeatability is good, and the quantitative standard curve is constructed without additional quantitative reference products, so that the operation is simplified and the utilization rate of the reagent is improved.

The invention provides qRT-PCR (quantitative real-time polymerase chain reaction) primers, a kit and a detection method for detecting tomato brown wrinkled fruit virus with high sensitivity, and belongs to the technical field of plant virus detection. The qRT-PCR primer for detecting the tomato brown wrinkled fruit virus, disclosed by the invention, is as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The invention also provides a qRT-PCR detection method of the tomato brown wrinkled fruit virus, the primer and the detection method provided by the invention have the characteristics of strong specificity and high sensitivity, the detection limit is 1.562 * 10 < 1 > copies / [mu] L, and the primer and the detection method have a better detection effect on a sample with low virus loading amount; and a technical support is provided for detecting the tomato brown wrinkled fruit virus and preventing and controlling the tomato brown wrinkled fruit virus.

The invention discloses application of KU55933 or pharmaceutically acceptable salts thereof in preparation of medicines for resisting bovine nodular skin disease virus infection and treating the bovine nodular skin disease virus infection, the KU55933 is utilized to incubate and treat MDBK, Vero and hTERT-CSF cells infected by LSDV, the influence of the KU55933 on LSDV infected susceptible cells is detected through Western blot and histiocyte half infection dose (TCID50), and the KU55933 or pharmaceutically acceptable salts thereof are used for preparing the medicines for treating the bovine nodular skin disease virus infection. It is found that after the ATM kinase specific inhibitor KU55933 is treated, LSDV proliferation in susceptible cells and extracellular cells of the LSDV can be remarkably inhibited; a body animal infection experiment also proves that the specific ATM kinase inhibitor KU55933 can effectively relieve clinical symptoms and skin tissue virus load of LSDV infected cattle. It is proved from the cell and animal level that the specific inhibitor targeting ATM kinase is expected to be applied to development of drugs for preventing or treating LSDV, and a new method is provided for prevention and control of bovine nodular dermatosis epidemic situation.

The utility model provides a kind of virus antigen rapid detection test paper, it is related to biological detection technical field, multi-dimensional gradient compound synergistic component, integrate dendritic flow guide groove and nano microporous structure on sample pad, it can promote processing liquid and sample to be radially evenly distributed in diffusion process, on the one hand, effectively avoid the detection interference caused by local liquid concentration too high, on the other hand, significantly shorten the liquid infiltration time of detection starting stage, improve chromatography efficiency, set up gradient distribution's antigen line on NC membrane, its virus specificity antigen concentration is increasing distribution from the end close to binding pad to bibulous pad, accurately adapt the detection requirement of different virus load sample, low virus load sample because of less combined compound, can flow to bibulous pad end high concentration antigen area sufficient combination and produce strong signal, avoid missed detection, high virus load sample because of compound sufficiency, preferentially and binding pad end low concentration antigen area quickly combine, effectively avoid signal inhibition or false negative problem.

The application discloses a single-tube quantitative detection HIV-1 virus load real-time fluorescent quantitative PCR kit and relates to the technical field of molecular biology detection. The kit comprises a primer and probe combination; the primer and probe combination comprises a primer pair GAG-1-F / R and a probe GAG-1-P for detecting a first target gene, a primer pair POL-1-F / R and a probe POL-1-P for detecting a second target gene, and probes IC-P1 and IC-P2 for detecting an internal standard. The kit covers various genotypes of HIV-1 viruses, can stably detect a virus load of 15 IU / mL, has high detection sensitivity and good repeatability, does not need additional quantitative reference products to construct a quantitative standard curve, can simplify operations, and improves the utilization rate of reagents.

The application discloses a kind of based on water body virus load and fish body tissue virus load correlation grouper nervous necrosis virus nondestructive testing method.The method is through optimizing the concentration condition of virus in aquaculture water, realize the efficient concentration and quantification of water body virus load, then combine water-water aquaculture animal virus load correlation model, indirectly and nondestructively assess the virus infection state of aquaculture animal.The application detects water environment instead of detecting animals itself, realizes nondestructive, dynamic, early warning monitoring of the health status of aquaculture animal population, with the advantages of low cost, simple operation, frequent implementation, etc., and has important application value in aquatic disease prevention and control, good seed selection and biological safety management.

Systems for detecting aerosolized viral loads in an airspace include a first and, optionally, a second volume of a liquid. The first volume includes particles sampled from the airspace, and the second volume is free of virus particles. A first conductivity probe is immersed in the first liquid, and a second conductivity probe is immersed in the second liquid. An alternating voltage is applied to each of the first and second conductivity probes. The difference between the resulting alternating currents through the first and second conductivity probes is determined, and is used to estimate the viral load in the first volume, and in the airspace.

Compositions and methods for inhibiting human immunodeficiency viral infection and for treating subjects already infected with a human immunodeficiency virus such as HIV-1. The compositions and methods reduce HIV viral load in subjects infected with HIV.

The invention discloses a veterinary epidemic prevention medicine as well as a preparation method and application thereof, and relates to the field of veterinary drug research and development and veterinary medicine, and the veterinary epidemic prevention medicine is prepared from the following components in parts by volume: 9.0-9.8 parts of Shuanghuanglian; 0.2 to 1.0 part of polyinosinic cell; through the molecular chaperone effect of the Shuanghuanglian and the polyinosinic-polycytidylic acid, the permeability and retention of the antiviral drug in myocardial tissues are remarkably improved, the technical bottleneck that a traditional drug is insufficient in myocardial virus load control is broken through, the damage of cytokine storm to myocardial cells is effectively inhibited while the antiviral immune response is enhanced, and the curative effect is good. The ratio of the Shuanghuanglian to the polyinosinic acid is optimized, the basic antiviral activity of the Shuanghuanglian is guaranteed, the immune regulation function of the polyinosinic acid is exerted with the minimum effective dose, the dose-dependent side effect is avoided, the medication process is simplified in clinical application, an innovative medicine combination with high efficiency, safety and economical efficiency is provided for the modern breeding industry, and the clinical application prospect is wide. And the method has remarkable industrial popularization value.

The application provides a multiplex fluorescence detection primer probe set and kit for the novel coronavirus omicron variant. The application has the following technical effects: 1. Low cost, the total reagent cost of a single specimen is 12 yuan. 2. Short detection time and simple operation, the experimental operation required by the application is more simple, and general reagents plus the primer probe working solution of the application can be detected on a machine, and can simultaneously judge SARS-CoV-2 infection and whether the omicron variant infection, the total detection time is about 1.5 hours. Simple operation process, conventional fluorescence detection instrument equipment, and the grass-roots unit can also carry out. 3. High sensitivity, which can be applied to environmental specimen detection, environmental specimen quantity is large, and virus load is low, which causes great difficulty to detection work. Due to the low virus load of the environmental sample, it is difficult to carry out high-throughput sequencing, and the method of the application can also be qualitative to the omicron variant when detecting the environmental sample.

The application belongs to the technical field of biology, and particularly relates to a 3D protein mutant O foot-and-mouth disease recombinant virus strain and construction and application thereof. The application mutates methionine at the 403th position of the 3D protein of the O foot-and-mouth disease virus into alanine, and successfully constructs and rescues a 3D protein mutant O foot-and-mouth disease recombinant virus strain rFMDV-M403A by using reverse genetic technology. The amino acid of the recombinant virus strain can be stably mutated in the process of subculture, and the growth speed of the mutant strain is slower than that of the wild strain, and the virus titer is significantly reduced. In individual experiments, the infection of the mutant strain to suckling mice shows that the mutant strain can improve the survival rate of the suckling mice, and significantly reduce the viral load in the liver and the tissue lesions. The application provides an attenuated foot-and-mouth disease recombinant virus strain, which has important significance for in-depth understanding of the pathogenic mechanism and prevention and control strategy of the foot-and-mouth disease virus, and provides an important theoretical basis for further development of the foot-and-mouth disease genetic engineering vaccine.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting capsicum vein mottle virus in tobacco, which comprises the following steps of: firstly, designing and synthesizing a specific primer pair based on a coat protein CP gene conserved sequence of capsicum vein mottle virus isolate in an NCBI (National Center of Biotechnology Information) database; secondly, extracting total RNA of a tobacco sample infected with the pepper vein mottle virus, and performing reverse transcription to obtain cDNA; carrying out real-time fluorescent quantitative PCR (Polymerase Chain Reaction) amplification by using the obtained cDNA as a template and adopting the specific primer pair; and finally, judging whether the sample carries the target virus or not according to the amplification curve, the melting curve and the Ct value, and realizing quantitative analysis of the virus load through the standard curve. The method is high in specificity and sensitivity, compared with a conventional PCR method, the sensitivity is improved by 10 times, and rapid and accurate detection of the pepper vein mottle virus in the tobacco can be achieved.

The invention belongs to the technical field of pathogen molecular diagnosis and high-throughput sequencing, and particularly relates to a novel bunyavirus whole genome capturing and sequencing method and kit for low virus load and high host background samples. Through the collaborative design of host nucleic acid reduction and two rounds of capture and enrichment, under the condition that the proportion of reads of an initial host source is greater than 90%, a high-coverage sequencing result of high-proportion base sites in L / M / S three fragments of the novel bunyavirus can still be obtained at a relatively low sequencing depth, the coverage integrity and the sequencing sensitivity of a whole genome are remarkably improved, and the method is suitable for large-scale popularization and application. And the sequencing cost and the result volatility are effectively reduced. Reliable whole genome data support can be provided for molecular typing, reassortment / recombination identification, aggregation epidemic situation analysis and transmission chain tracing of the novel bunyavirus, and the method has important clinical and public health application value.

The invention discloses a capture probe for detecting human immunodeficiency virus type 1, a plurality of capture probes are obtained by adopting imbricated design according to a plurality of reference genome whole-genome sequences of standard strains and subtype strains of HIV in an NCBI database, after a total RNA library is constructed, the capture probes are used for capturing the HIV whole-genome library, and the HIV whole-genome library is used for detecting the human immunodeficiency virus type 1. The probe sequence densely and repeatedly covers all areas of each reference genome, and the design parameters of the probe are as follows: the length is 120nt, and the imbrication frequency is 3X-6X Tiling. The invention further provides a kit comprising the capture probe and application of the kit. According to the capture probe, sequencing data is optimized, invalid data redundancy is reduced, sequencing efficiency is improved, and sequencing cost is reduced. Through whole genome sequencing, drug-resistant mutation and low-frequency mutation of a low-load sample can be detected, typing and virus load can be detected at the same time, the detection cost is remarkably reduced, and the detection efficiency is improved.

The invention belongs to the technical field of aquatic immunity and biological preparations, and discloses a CXCL20a protein mutant and application of the CXCL20a protein mutant as a grass carp reovirus inactivated vaccine adjuvant. In order to improve the immunomodulatory activity of CXCL20a, the applicant constructs and obtains a CXCL20a mutant protein. After the mutant and a GCRV-II type inactivated vaccine are combined to immunize grass carp, the expression of immune related genes can be remarkably promoted, the activity of serum acid phosphatase and superoxide dismutase is enhanced, and meanwhile, the level of lactic dehydrogenase is reduced. A challenge experiment proves that the survival rate of the grass carp combined with the inactivated vaccine for immunization is increased by about 8% compared with that of a non-mutant combined group, and the in-vivo virus load is reduced. As an immunologic adjuvant, the CXCL20a mutant M-C20 provided by the invention can effectively enhance early immune response and overall protection effect in vaccine immunity, and has good safety, stability and application prospect.

The invention discloses a stable F protein vaccine composition before fusion of a recombinant respiratory syncytial virus. According to the vaccine composition provided by the invention, a stable pre-fusion trimer structure can be maintained in the storage process of the recombinant pre-fusion F protein, and the combination with D25, AM14 and other antibodies with efficient RSV virus neutralizing activity can be maintained; besides, the vaccine composition can induce a high-titer binding antibody and a high-titer neutralizing antibody on a mouse model under the condition that a vaccine adjuvant is not added, and the lung tissue virus load can be remarkably reduced in a challenge experiment. Therefore, the vaccine composition has a good immune protection effect on the respiratory syncytial virus.

The application discloses an application of an iPLA2 beta inhibitor in preparation of a medicine for preventing and / or treating zika virus infection, and belongs to the technical field of biological medicine. The iPLA2 beta inhibitor is preferably FKGK18, which can inhibit replication of zika virus in host cells, and reduce virus RNA level and virus protein expression level. Animal experiments show that FKGK18 administration can significantly reduce virus load in brain tissues of infected animals, reduce glial cell activation, and inhibit expression of neuroinflammatory factors, thereby reducing zika virus infection related neuroinflammatory reaction and neuron damage. The application further provides a pharmaceutical composition containing the iPLA2 beta inhibitor, and a scheme of combined use of the iPLA2 beta inhibitor and PE-DHA. The application provides a new drug intervention target and candidate drug for prevention and treatment of zika virus infection and related nervous system damage, and has a good clinical application prospect.

The invention discloses a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method and a kit for simultaneously detecting IMNV (Immunodeficiency Virus), CMNV (Cytomegalovirus) and DIV1 (Deoxyribose Immunodeficiency Virus According to the invention, PCR primers and TaqMan probes for detecting IMNV, CMNV and DIV1 are respectively designed, and the nucleotide sequences of the PCR primers and TaqMan probes are shown as SEQ ID NO: 1-9. The primer and the probe can be used for simultaneously qualitatively and quantitatively detecting the infectious muscle necrosis virus, the secretly death nodavirus and the full-eye iridovirus 1 through a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method. According to the invention, common detection of RNA virus and DNA virus is realized for the first time, the kit has the characteristics of high efficiency and convenience, and the advantages of high sensitivity, high specificity and quantitability, and meanwhile, due to the addition of the molecular crowding agent, the detection efficiency and detection speed of medium and low-concentration virus load can be effectively improved, and the detection cost is reduced. The invention provides a convenient and reliable detection method and product for early monitoring, early warning and diagnosis of the three virus diseases of the penaeus vannamei boone.

The invention relates to the technical field of single cell transcriptomics and microbiomics, in particular to a method for detecting viral load based on single cell transcriptome sequencing, which comprises the following steps: extracting single cell transcriptome sequencing data to form a single cell gene expression matrix, and comparing the single cell gene expression matrix with a host comparison file to extract reading data; dynamically translating the reading data to generate an amino acid sequence; comparing and scoring the amino acid sequence with a virus protein sequence in a virus database, obtaining a weighted comparison value through distance weight correction, and forming a virus sequence-cell matrix by the weighted comparison value and reading data; inputting the virus reading data, the host cell immune response index and the cell type sensitivity score into a cell infection tendency index calculation formula, and obtaining the cell infection probability through a logistic regression model. The technical problem that the sensitivity to RNA viruses with high mutation rate is poor is solved, quantitative estimation of the single cell infection probability is achieved, and the method is suitable for detecting distant viruses or new viruses which are not recorded yet.

The invention discloses an application of a ferroptosis inhibitor in preparation of a novel bunyavirus resisting drug. In-vivo and in-vitro experiments find that the ferroptosis inhibitor Liproxstatin-1 or DFO can reduce ferroptosis, virus load and MDA (malondialdehyde) content caused by novel bunyavirus infection by improving iron overload, lipid peroxidation and cell death, so that multi-tissue organ injury caused by novel bunyavirus infection is relieved, and the novel bunyavirus is inhibited. Therefore, the ferroptosis inhibitor can be used for preparing the medicine for preventing and / or treating the novel bunyavirus disease.

The invention relates to the technical field of virus quantitative detection and analysis, in particular to a radix pseudostellariae TuMV and BBWV2 virus quantitative detection method and a rapid analysis program thereof. The method comprises the following steps: preparing a virion standard substance of a virus type to be detected, diluting the virion standard substance by 10 times of gradient, carrying out TaqMan qPCR amplification on the standard substance subjected to gradient dilution to obtain a mapping relationship between a corresponding Cq value and a logarithm of an initial copy number, fitting a standard curve relational expression by utilizing the mapping relationship, establishing a VBA data processing module by utilizing a VBA code of Excel, and carrying out quantitative analysis on the VBA data. The fitting standard curve relational expression is written into a program, when a user subsequently detects various virus loads of a radix pseudostellariae sample, the corresponding virus load result can be quickly output only by utilizing primers of corresponding viruses to respectively carry out TaqMan qPCR amplification and importing a plurality of Cq values into a VBA data processing module, and the method has the characteristics of simplicity, high efficiency and accuracy.