50 results about "Virus load" patented technology

The present invention provides methods for treating alphavirus infections; methods of treating hepatitis C virus (HCV) infections; methods of treating West Nile virus infection; methods of reducing liver fibrosis; methods of increasing liver function in an individual suffering from liver fibrosis; methods of reducing the incidence of complications associated with HCV and cirrhosis of the liver; and methods of reducing viral load, or reducing the time to viral clearance, or reducing morbidity or mortality in the clinical outcomes, in patients suffering from viral infection. The methods generally involve administering effective amounts of an interferon receptor agonist and pirfenidone in combination therapy.

The invention discloses a method for detecting HBVDNA through combination of PCR and CRISPR. The method comprises the steps of (1) amplifying nucleic acid of samples to be detected through a pair of upstream and downstream specific primers, wherein a sequence which can be recognized and transcribed by T7RNA polymerase is arranged at a 5' end of the downstream primer; and (2) detecting whether a targeting sequence exists or not in amplification products of the nucleic acid of the samples to be detected in a detection system containing crRNA, T7RNA polymerase, Cas13a protein and report RNA for recognizing an HBVDNA target sequence, wherein the section of the HBVDNA target sequence is 803<rd>-829nt. The invention further discloses crRNA capable of targeting HBVDNA specific sites and a reagent kit containing the crRNA. The method provided by the invention is simple, convenient and quick during detection of the content of the HBVDNA, and has extreme high sensitivity and specificity, single-copy HBV DNA is differentiated within 15 minutes after PCR amplification, and higher positive detection rate can be shown for serum samples low in virus load.

The present invention provides methods for lowering a viral load of a virus resistant to an antiviral drug by inducing cytotoxic T lymphocytes (CTL) to recognize a predetermined mutated epitope within a viral protein of the drug-resistant virus. CTLs are induced by immunizing a host with a peptide comprising the predetermined mutation. The immunostimulating peptide may be further improved by epitope-enhancement for inducing specific CTLs. The antiviral protection against drug-resistant virus shown by compositions of the present invention and mediated by human HLA-restricted CTL has not been previously achieved.

The invention discloses a single standard product-based quardruple four-color fluorogenic quantitative PCR (Polymerase Chain Reaction) technology. The technology is capable of detecting group A rotavirus (HRVA), norovirus group I (NVGI), norovirus group II (NVG II) and human astrovirus (HAstV) RNA in various treated specimen through one-step quardruple PCR amplification and quantifying virus load. The technology is characterized in that single standard product is adopted as positive control and quantitative standard in the quardruple four-color fluorogenic quantitative PCR detection for four viruses, thereby breaking through the deficiency that the traditional multiple fluorogenic quantitative PCR needs a plurality of standard products, improving the experiment detection efficiency, reducing the pollution probability and improving the preparation and production efficiency of the kit. The method and kit can be used for fast high-throughput test and epidemic monitoring in laboratories of HRVA, NVG I, NVG II and HAstV which can cause diarrhea syndromes. The single standard product-based four-color florogenic quantitative PCR method and the kit belong to the biotechnology field.

Methods of reducing hepatitis delta virus (HDV) viral loads in a patient are provided. In some embodiments, the method comprises treating the patient with lonafarnib-ritonavir co-therapy. In some embodiments, the method further comprises treating the patient with an interferon.

The invention relates to a plasmid, system and preparation for targeted knockout of human papilloma virus (HPV) URR (upstream regulatory region) genes and a preparation method of the plasmid, system and preparation. The plasmid comprises sgRNA, Cas9 and an expression vector PX330 which can bind to the HPV URR genes. When transfected into cells, the plasmid specifically induces coding shift mutations of HPV oncogenic elements in corresponding HPV subtype positive cells to loss carcinogenic properties, even too much DSB can lead to cell apoptosis to reduce virus load, remove virus and pathological cells and reverse cancerous transformation, and important clinical application value is achieved. The preparation can effectively kill HPV positive tumor cells and effectively treat HPV positive tumor cells and is simple in preparation and low in cost.

The invention relates to a novel application of IFN-lambda in Zika virus infection and particularly provides an application of IFN-lambda 2 in preparation of a drug for preventing or treating Zika virus infection. Tests prove that delayed growth of fetal rats and placenta injury due to the Zika virus can be relieved by means of IFN-lambda 2, the relative Zika viral load in all organs of a mother rat and the fetal rats is detected through q-PCR (quantitative polymerase chain reaction), and the fact that IFN-lambda 2 can inhibit reproduction of the Zika virus in the mother rat and the fetal rats is further verified. Besides, the invention further illustrates a mechanism of the IFN-lambda 2 in inhibiting Zika virus reproduction by the means of cytobiology. A new way and method are provided for preventing or treating microcephalus caused by Zika virus infection.

The invention relates to a test method in the bioengineering field, in particular to a fluorescence quantitative PCR test method used for quantitatively test the titer of Hepatitis E virus (HEV) in a clinical specimen. The steps of invention include the design of virus specific quantitative PCR primers and probes, the construction of standard molecular, the extraction of a virus sample RNA, the cDNA synthesis of the sample RNA and the establishment and optimization of the fluorescence quantitative PCR test method. The testing sensitivity of the invention can reach to 10 degrees magnitude virus copy. The invention is equipped with good stability and specificity, which can be used for mass samples test and is capable of precisely measuring the virus load of the sample.

The present invention provides a chimeric HIV-1 construct, EcoHIV, capable of replication in a rodent cell. The invention also provides a convenient and safe rodent model of HIV-1 infection and AIDS. Methods for producing a rodent model of HIV-1 infection are also provided. Additionally, the invention provides the means to test immunogenic compositions or pharmaceutical interventions effective in preventing infection, reducing viral load, or reducing disease symptoms in a subject.

The present invention provides methods of treating hepatitis C virus (HCV) infection; methods of reducing the incidence of complications associated with HCV and cirrhosis of the liver; and methods of reducing viral load, or reducing the time to viral clearance, or reducing morbidity or mortality in the clinical outcomes, in patients suffering from HCV infection. The methods generally involve administering to the individual i) a Type I interferon receptor agonist or a Type III interferon receptor agonist; ii) an immunomodulatory agent; and iii) an inhibitor of an HCV enzyme.

This method provides a method for reducing HIV-I viral load in an HIV-1-infected human subject which comprises administering to the subject at a predefined interval effective HIV-I viral load- reducing doses of (a) a humanized antibody designated PRO 140, or of (b) an anti-CCR5 receptor monoclonal antibody. This invention also provides a method for inhibiting in a human subject the onset or progression of an HIV-I -associated disorder, the inhibition of which is effected by inhibiting fusion of HIV-I to CCR5CD4 target cells in the subject. This invention also provides a method for treating a subject infected with HIV-I comprising administering to the subject (a) a monoclonal antibody which (i) binds to a CCR5 receptor on the surface of the subject's CD4 cells and (ii) inhibits fusion of HIV-I to the subject's CCR5CD4 cells, and (b) a non-antibody CCR5 receptor antagonist, in amounts effective to treat the subject.

The present invention provides methods of treating hepatitis C virus (HCV) infection; methods of reducing the incidence of complications associated with HCV and cirrhosis of the liver; and methods of reducing viral load, or reducing the time to viral clearance, or reducing morbidity or mortality in the clinical outcomes, in patients suffering from HCV infection. Also provided are methods of treating liver steatosis and liver fibrosis.

The invention discloses a bivalent inactivated vaccine for porcine reproductive and respiratory syndrome and a preparation method thereof. The bivalent inactivated vaccine for the porcine reproductive and respiratory syndrome comprises the following two inactivated viruses: a porcine reproductive and respiratory syndrome virus with the strain number of PRRSV-ZJ/H strain and the preservation number of CCTCC NO: V202130, and a porcine reproductive and respiratory syndrome virus with the strain number of PRRSV-ZJ/NB strain and the preservation number of CCTCC NO: V202140. The bivalent inactivated vaccine prepared by the invention can effectively prevent and control two major prevalent PRRS virus types in the current field, and a needle of the PRRS inactivated vaccine is immunized in a commercial pig field which is naturally infected or is immunized by using a PRRS attenuated live vaccine, so that the virus specificity neutralizing antibody can be obviously improved, the virus load in the infected pig body can be reduced, the PRRS epidemic situation can be quickly and effectively controlled, the daily gain of pigs can be obviously improved, and the economic benefits of pig farms are increased.

The invention provides an application of macromolecular protein in an anti-RNA virus disinfectant. The macromolecular protein is one or more of natural immune polypeptide and protein secreted by bacteria, fungi, animals or plants in the biological field, the disinfectant acts on the body surface environment, the in-vitro environment or the oral and nasal cavity environment, and the macromolecularprotein is human RNase 2 and/or RNase 3. The RNase 2 and RNase 3 in the disinfectant are very stable in structure and lasting in activity, can inhibit viruses in vitro and directly destroy virus outermembranes, and meanwhile can degrade virus genomes, and can reduce the infection virus load in the in-vitro environment; compared with the existing small molecule disinfectant, the disinfectant has the advantages of long action time, high efficiency, no irritation to human bodies, environmental protection, cyclic effect exertion and the like, and is expected to have great application prospects inthe aspect of prevention of respiratory tract and gastrointestinal tract RNA virus infection of novel coronavirus, influenza virus and the like.

The invention discloses an application of geraniin to preparation of a medicine for restraining novel coronaviruses. The geraniin is applied to treatment of infection caused by the novel coronavirusesfor the first time, and through research, the inventor finds that the geraniin can effectively inhibit proliferation of the novel coronaviruses in cells, so that the geraniin can be used for treatingpneumonia diseases caused by infection of the novel coronaviruses. Therefore, the application range of the geraniin is expanded, and a new thought is provided for prevention and treatment of the novel coronaviruses. Through research, the inventor finds that 12.5-100 [mu] g/mL of the geraniin can effectively inhibit proliferation of SARS-CoV-2 in cells. Compared with virus infected cells (virus control group) which are not treated by any medicines, after the virus infected cells are treated by 100[mu]g/mL of the geraniin for 48 hours, the intracellular SARS-CoV-2 virus load is averagely reduced by 8.66 * 10<2> times.

Processes for separating a viral load from a pancreatin sample and for quantitatively determining the viral load in a pancreatin sample with high sensitivity are described herein.

The present invention provides novel and non-obvious improvements to dried blood spot testing for HIV-1 viral load useful for diagnosis and monitoring treatment progression.

The invention discloses an anti-HPV infection medicine, which comprises the following raw material medicines in parts by weight: 10-20 parts of radix sophorae flavescentis, 10-20 parts of phellodendron amurense, 6-12 parts by weight of cortex dictamn, 4-8 parts by weight of safflower; 2-4 parts by weight of fructus cnidii; 3-5 parts by weight of Radix Astragali; 2-4 parts by weight of aloe; and 0.5-3 parts by weight of bunge corydalis herb. The invention discloses the anti-HPV infection medicine, the clinical symptoms and signs of patients can be obviously improved; the virus load capacity ofa virus infected person is effectively reduced; the medicine for treating the heart disease is prepared from the following raw materials: radix sophorae flavescentis, phellodendron amurense, cortex dictamni, safflower, fructus cnidii, Radix Astragali, aloe, bunge corydalis herb, borneol, perilla stems, eupatorium, campsis grandiflora, ground beetle, cortex magnoliae officinalis, rhinacanthus nasutus and rhizoma alismatis. Through reasonable compatibility of all the raw materials, the special performance and effects of all the raw materials are fully exerted, meanwhile, the complementary effectof all the raw materials is considered, the medicine effects supplement each other, the condyloma acuminatum treatment effect is remarkable, and no toxic or side effect exists.