51 results about "Cyclovirus" patented technology

The present invention belong to veterinary new biological medicine technology field, relates to porcine circovirus type II (PCV2) inactivated vaccine of and method for preparing same. The vaccine used seed virus is porcine circovirus type II DBN-SX07 strain, the preservation number is CGMCC No 3064, the virus strain is used as antigen preparation of inactivation by alkyl agents and emulsification by adding oil adjuvant. Using the invention provided PCV2 inactivated vaccine to immune pig can generate an uniform and effective protection force-shorting PCV2 infection windows period obviously, and prolong immune duration-reducing times of booster immunization.

A chimeric hog cyclovirus PCV1-2 is disclosed. Its configuring method include ssuch steps as using the PVC2-ORF2 of hog cyclovirus PVC2 to replace the PVC1-ORF2 of PVC1, configuring a fragment of deficiency PCV1-ORF2 genome (pSK-PVC1 delta ORF2), and linking it to a fragment of PCV2-ORF2 genome. It can be used to prevent and treat the multi system failure syndrome of delactated piglet by PCV1-2 infectious immunizing cesarean section and deprivating foremilk.

The invention discloses colloidal gold rapid diagnosis test paper of a porcine 2 type torque teno virus antibody and a preparation method thereof. The test paper comprises a PVC (Poly Vinyl Chloride) baseplate, a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and water absorbing filter paper, wherein the sample pad, the colloidal gold combination pad, the nitrocellulose membrane and the water absorbing filter paper are sequentially arranged on the PVC baseplate in the chromatography direction; one end of the sample pad is folded at the front end of the colloidal gold combination pad; the back end of the colloidal gold combination pad is folded at one end of the nitrocellulose membrane; the front end of the water absorbing filter paper is folded at the other end of the nitrocellulose membrane; a detection line and a quality control line are respectively sprayed on the nitrocellulose membrane; and the colloidal gold combination pad is composed of a layer of nitrocellulose membrane and rTTSuV2ORF1' colloidal gold sprayed on the nitrocellulose membrane. The colloidal gold rapid diagnosis test paper of the porcine 2 type torque teno virus antibody, disclosed by the invention, is capable of rapidly detecting the TTSuV2 antibody and has the advantages of simple structure, convenience in use and good sensitivity and specificity.

The invention discloses a primer pair for detecting pigeon torque teno viruses. The nucleotide sequence of the primer pair is represented by SEQID NO:2 and SEQ ID NO:3. The invention also discloses a nucleotide sequence of the pigeon torque teno viruses and a preparation method for the nucleotide sequence. The nucleotide sequence of the virus is obtained by performing polymerase chain reaction (PCR) on the primer pair and is represented by SEQ ID NO:1. The invention also discloses a detection kit which comprises the primer pair and is used for detecting the pigeon torque teno viruses and a detection method. According to the primer pair for detecting the pigeon torque teno viruses, on the basis of a specific primer pair, a PCR condition is optimized; the nucleotide sequence of the pigeon torque teno viruses is amplified; and a detection condition and a detection system are optimized, so that the kit for detecting the virus is prepared. The kit is relatively high in specificity and stability; the sensitivity of the kit is up to 5 pg; and the kit can quickly and accurately detect the pigeon torque teno viruses. The kit can be used as an effective tool for quickly detecting the pigeon torque teno viruses.

The invention relates to a method for preparing porcine circovirus type 2 inactivated vaccine by utilizing a bioreactor, wherein the bioreactor is a riptide type bioreactor; a circulatory system is composed of a perfusion bag and a riptide bag; a cell culture bag is built in the perfusion bag; a paper carrier is in the cell culture bag; and the method comprises the following steps of: culturing PK-15 cells; inoculating porcine circovirus type 2; culturing the porcine circovirus type 2, measuring sugar consumption by taking a virus maintenance solution, and obtaining a virus solution when the sugar consumption is decreased; and preparing the porcine circovirus type 2 inactivated vaccine. The method for preparing the porcine circovirus type 2 inactivated vaccine by utilizing the riptide type bioreactor disclosed by the invention is small in production site, low in labour intensity, relatively lower in production cost, simple in operation, low in inter-batch difference, and high and steady in product quality; full-automatic micro-computer control can be achieved; and the prepared porcine circovirus type 2 inactivated vaccine is good in safety, high in immune efficacy and low in side effect and has the better immune protective effect on virulent attack of porcine circovirus type 2.

The invention discloses a traditional Chinese drug composition for pig and a preparation method and application thereof. The composition comprises drug substances of 6-18 parts of indigofera tinctoria by weight, 4-12 parts of Chinese amphibious knotweed rhizome by weight and 5-15 parts of Chinese mosla herb by weight. The preparation method of the composition comprises: distilling Chinese mosla herb, and collecting the distillate; adding indigofera tinctoria and Chinese amphibious knotweed rhizome in the residue and residual liquid after distillation and decocting for 2-3 times, mixing the decoctive solution and concentrating the solution to viscous liquid, adjusting an alcohol concentration of ethanol to be 60 percent to 70 percent, precipitating, filtrating and concentrating the liquor, adding ethanol and adjusting the alcohol concentration to be 75 percent to 80 percent, sequentially performing precipitation, filtration and concentration of filter liquor, and adding distilled water and refrigerating for precipitation, filtration, and concentration; and blending the distillate of Chinese mosla herb with the collected concentrated solution of indigofera tinctoria and Chinese amphibious knotweed rhizome. The Chinese drug composition can be used for preparation of traditional Chinese veterinary drug to treat blue ear pig disease, porcine circovirus infection, pseudo rabies, piglet pullorum disease of piglet, paratyphoid of piglet, pig erysipelas and edema disease of piglet. Moreover, the Chinese drug composition has the advantages of few side effect and safe and convenient use.

The invention relates to a gene chip for a detecting porcine respiratory disease complex virus and a method for detecting the porcine respiratory disease complex virus, wherein the gene chip comprises a solid phase carrier, and an oligonucleotide probe and a quality control oligonucleotide probe which are fixed on the carrier. The oligonucleotide probe comprises DNA sequences selected from an NPS2 gene of porcine reproductive and respiratory syndrome virus, gene among circovirus II type genome sequence 990-1500bp, gE gene and pK gene of pseudorabies, HA, NA and M genes of swine influenza virus H1N1, and HA and NA genes of swine influenza virus H3N2. After a to-be-detected sample genomic DNA is amplified and labeled by utilizing a designed primer, the gene chip is used for hybridization, and the porcine respiratory disease complex virus can be detected according to the hybridization signals. According to the gene chip and the detection technology of the gene chip, four samples and five viruses can be simultaneously detected on the same one chip, and the diagnosis efficiency can be greatly improved.

The invention relates to a multivalent vaccine for preventing and treating the infectious diseases of a pig, in particular to a combined vaccine for treating and preventing porcine circovirus type 2 and porcine parvovirus and a preparation method thereof. The multivalent vaccine selects and uses the porcine circovirus type 2 and the porcine parvovirus. The preparation method comprises the following steps of: culturing the porcine circovirus type 2, inactivating and concentrating; culturing the porcine parvovirus, inactivating and concentrating; mixing the two antigen components by proportion, and adding adjuvants to prepare into the vaccine. The bivalent vaccine prepared by the invention is convenient in use and is safer, and the immune effect is superior to that ofcombination of two single vaccines.

The invention relates to the field of epidemic prevention detection, in particular to a method, primer, probe and kit for detecting porcine circovirus through triple digital microdroplet PCR. According to an application of a detection target spot to detection of the porcine circovirus, the detection is a non-disease detection and diagnosis method, and the detection target spot comprises one or more of the following detection target spots: the sequence of the porcine circovirus type 1 (PCV1) detection target spot is shown as SEQ ID NO: 1; the sequence of a porcine circovirus type 2 (PCV2) detection target is as shown in SEQ ID NO: 2; and the sequence of a porcine circovirus type 3 (PCV3) detection target is as shown in SEQ ID NO: 3. Based on the advantages of ddPCR, a high-sensitivity and accurate identification and detection method for different genotypes of the porcine circovirus is established, and the method can be applied to conventional animal quarantine and quarantine monitoring and load titration of animal-derived products with low virus content and complex matrixes such as feeds, meat products and vaccines.

The invention discloses a traditional Chinese medicine composition for pigs and a preparation method and application of the traditional Chinese medicine composition for pigs. The composition is prepared from, by weight 6-18 parts of Fructus Broussonetiae, 15-45 parts of Schizomussaenda Dehiscens, 9-27 parts of Radix Mahoniae and 8-24 parts of Rhapis Excelsa. The preparation method includes: mixing the four traditional Chinese medicines, adding water, soaking, decocting for 2-3 times, combining decoctions, concentrating to a thick state, using ethyl alcohol to adjust the alcohol concentrate to 60%-70%, precipitating, filtering, adding ethyl alcohol to concentrated medicine liquid to adjust the alcohol concentration to 75%-80%, precipitating, filtering, concentrating filtrate, adding distilled water, refrigerating, precipitating, filtering, and concentrating to obtain a required dosage form. The traditional Chinese medicine composition is used for preparation of Chinese veterinary drugs for treating blue ear disease, porcine circovirus disease, pseudorabies, necrotic enteritis, swine enzootic pneumonia and haemophilus parasuis and is free of evident toxic and side effects and safe and convenient to use.

The invention belongs to the technical field of virus detection, and specifically relates to a real-time fluorescent quantitative PCR detection primer probe set, kit and method of porcine circovirus type 3. The detection accuracy is enhanced by designing primers on PCV3 cap protein in the invention for the first time; a fully closed tube operation is achieved in the detection method, the occurrence of false positive phenomenon is reduced, and at the same time, the high specificity of TaqMan probe technology and the high sensitivity of spectroscopy technology not only overcome the shortcoming of conventional PCR technology that can only achieve qualitative detection, but also solve the shortcoming of an SYBR Green method with poor specificity, and the detection method is more suitable for clinical testing; and the method has high sensitivity, good specificity and good reproducibility, the lowest detection limit is 11.8 copies/[mu]L, and the detection method is more sensitive than otherfluorescent quantitative PCR detection methods.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer group for visual detection on infection conditions of duck circovirus (DuCV) and goose circovirus (GoCV) and a method thereof. According to the method, the detection on the infection conditions of DuCV and GoCV is carried out by using dissolution curve temperature Tm value difference caused by the difference between nucleotide GC contents of DuCV and GoCV specific gene fragment regions amplified by primers, and the infection conditions of DuCV and GoCV can be subjected to visual differential diagnosis specifically by only combining the SYBR Green I based real-time fluorescent quantitative PCR primer group to difference of dissolution curve Tm values which are automatically generated after reaction is ended. The method disclosed by the invention is simple and is relatively high in efficiency and accuracy.

The invention provides a preparation method of a phage display-expressing circovirus antigen vaccine. The preparation method comprises the following steps: extracting porcine circovirus II type (PCV2) RNA, carrying out inverse transcription, carrying out a polymerase chain reaction (PCR) for amplification so as to obtain porcine II type circovirus ORF2 gene's open reading frame fragments, directionally inserting the fragments into a T4 phage; obtaining a T4 phage with the ORF2 gene by an immunoscreening method; purifying and infecting X-Blue ST1(CCTCC M 2014397) host cell again; carrying out fermental cultivation to obtain the X-Blue ST1 host cell, and carrying out inducible expression and inactivation and taking a culture as an antigen; carrying out ELISA calibration of antigen valence, and carrying out double dilution on the antigen with normal saline to the final concentration of 107U/ml; and a vaccine adjuvant is added according to the ratio of 1:2.5, so as to obtain the vaccine. According to the invention, the phage is utilized to express the porcine circovirus II type antigen and the vaccine is prepared. Thus, the virulence enhancement risk of an attenuated vaccine is avoided. In addition, fermental cultivation is beneficial to large-scale industrial production. Cost of the vaccine provided by the invention is far lower than that of a vaccine produced by a cell culture method.

The invention discloses a fluorescent quantitative PCR primer group and kit for detecting European eel circovirus, the kit comprises the primer group, the primer group comprises a forward primer F, a reverse primer R and a fluorescent probe Q. The kit also comprises DNA polymerase, UDG enzyme, a 2 * reaction buffer solution, a sealing solution, a positive reference substance and a negative reference substance. The kit provided by the invention can directly detect the European eel circovirus from a complex sample, has the advantages of strong specificity, high sensitivity, good experimental repeatability and convenience and rapidness in operation, can complete detection without expensive instruments, and can be used for on-site rapid and accurate detection of the European eel circovirus.