11 results about "Cyclovirus" patented technology

The invention relates to the technical field of molecular biological detection. The invention provides a specific primer group and a specific probe for simultaneously detecting and distinguishing six torque teno virus subtypes. The six torque teno virus subtypes comprise a TTV-29 subtype, a TTV-16 subtype, a TTV-15 subtype, a TTV-24 subtype, a TTV-1 subtype and a TTV-19 subtype. The specific primer group and the specific probe are used for qPCR amplification, the six most common torque teno virus subtypes in HSCT patients can be detected and distinguished at the same time, the detection speed is high, the specificity is high, and the problems that in the prior art, the detection efficiency is low, and the typing capacity is insufficient are effectively solved; meanwhile, the detection method is simple, short in detection time, low in cost, high in accuracy and easier to accept by patients.

The invention discloses a novel indoor identification method for the ringspot virus resistance of a new transgenic papaya variety, and belongs to the technical field of plant virus inoculation. According to the method, a sterile steel nail brush is used for dipping inoculation liquid containing ringspot viruses, indoor cultivated papaya leaves are inoculated, cultivation is conducted for 10 days after inoculation is completed, disease investigation is conducted on transgenic papaya, and the transgenic papaya is obtained according to the disease index of the inoculated transgenic papaya. And identifying the resistance of the transgenic papaya to the ringspot virus. The inoculation method adopted by the invention is simple and convenient to operate, has the advantages of high inoculation efficiency and good repeatability, can realize rapid batch inoculation of the ringspot virus on the papaya, and is not only suitable for screening new varieties of transgenic papaya resistant to ringspot, but also suitable for screening new varieties of transgenic papaya resistant to ringspot. And the method also has important significance for exploring the pathogenic mechanism of the ringspot virus and the disease-resistant mechanism of disease-resistant papaya varieties.

The invention relates to the technical field of medicine, in particular to a porcine circovirus type 3 Cap recombinant protein and a matched detection method and application thereof.According to the porcine circovirus type 3 Cap recombinant protein, efficient and high-quality preparation is achieved through gene optimization and expression process improvement, Escherichia coli rare codons in ORF2 genes are replaced with GeneOptimizer software, an unfavorable structure rich in arginine at the N end is removed, and the recombinant protein is obtained. The mRNA stability and the translation efficiency are obviously improved; through IPTG induction condition optimization and'inclusion body treatment-Ni-NTA affinity chromatography-dialysis renaturation 'purification processes, the final protein purity exceeds 95%, a high-quality material basis is provided for subsequent detection, the matched indirect ELISA detection method has the core characteristics of'accuracy, high efficiency and stability', detection can be completed within 2-3 h, synchronous analysis of large-batch samples is supported, and the detection efficiency is greatly improved.

The invention relates to the technical field of medical examination and clinical prediction models. The invention provides an application of a system for detecting torque teno virus load in preparation of a product for predicting or assisting in predicting the condition of a hematopoietic stem cell transplantation patient, and a risk assessment model for predicting the outcome of HSCT patient infection, graft versus host disease, relapse, non-relapse death and the like is established by detecting the dynamic change of torque teno virus load. Therefore, early warning and risk layering of the illness state of the patient before clinical symptoms appear are realized, and the accuracy of patient management and treatment is improved.

The present invention relates to the technical field of in vitro detection reagents, and specifically to a porcine circovirus antibody detection kit based on an indirect immunofluorescence method and its application. The porcine circovirus Cap antigen provided by the present invention can simultaneously bind to serum antibodies induced by two genotypes, TTSuV1 and TTSuV2, and has high specificity. Based on the Cap antigen and the IFA method, the present invention establishes a serological detection kit and detection method for TTSuV antibodies, which can simultaneously detect serum antibodies induced by two genotype viruses, TTSuV1 and TTSuV2, and has good specificity, stability and repeatability, effectively avoiding the problems encountered in the establishment process of the ELISA method, and can better meet the needs of TTSuV serological clinical detection, and has good application prospects.

The invention provides a stirring type reactor culture process of a porcine circovirus type 2 recombinant baculovirus, which comprises the following steps: preparing a seed virus of the porcine circovirus type 2 recombinant baculovirus by using an insect cell Sf9 full-suspension culture process, and inoculating an insect cell High Five by using the seed virus prepared by using an Sf9 cell to perform antigen expression of the porcine circovirus type 2 recombinant baculovirus. A full-suspension culture mode is utilized, so that the labor intensity is reduced, the production cost is reduced, and pollution is not easily caused; 2) the risk of porcine virus pollution can be reduced by using insect cells and a baculovirus expression system; and 3) the characteristics (Sf9 and High Five) of different insect cells are simultaneously utilized to respectively carry out seed virus preparation and antigen expression, so that higher virus titer can be obtained, the virus titer is greater than or equal to 7.50 Log (TCID50 / mL), higher recombinant protein expression is obtained, and the protein expression quantity is greater than or equal to 150 mu g / mL.

The invention relates to the technical field of molecular biological detection, and particularly discloses a kit capable of simultaneously detecting eight porcine respiratory pathogens and application. The invention specifically relates to detection of hog cholera virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, circovirus type 2, African hog cholera virus, swine influenza A virus, streptococcus suis and swine pasteurella multocida. According to the combined detection composition provided by the invention, a dual quadruple fluorescent PCR analysis method is utilized, and different pathogens are detected by detecting target spots on the different pathogens, so that target genes of eight pathogens can be rapidly detected at a time, and detection and differential diagnosis are more efficient, economical, simple and convenient; the kit has the advantages of high sensitivity, good specificity, rapidness, high flux, simplicity and convenience in operation and the like.

The application discloses a kind of graft injury multi-index combined noninvasive detection kit, by optimized multiplex real-time fluorescent quantitative polymerase chain reaction technology, realize the quantitative detection of six categories with closely related DNA virus after transplantation Complications, the present application relates to the field of in vitro detection technology of clinical sample.The graft injury multi-index combined noninvasive detection kit, the present application first realizes the synchronous detection of BK virus, JC virus, B19 virus, CMV virus, adenovirus and six kinds of closely related viruses of circovirus (TTV) with graft injury.Test data see example 7, from the detection results of 100 clinical kidney transplant patients, it can be seen that the kit of the present application can simultaneously accurately detect the load level of six viruses, to provide comprehensive infection risk assessment for clinicians.Compared with the prior art single virus detection method, the present application avoids the cumbersome process of multiple sampling and detection, significantly improves the clinical work efficiency.

The invention belongs to the field of immunology and genetic engineering, and particularly relates to a preparation method and application of a porcine circovirus 2d subtype strain inactivated vaccine. The PCV2d subtype strain obtained through separation is proliferated in a PK-15 cell of overexpressed NPM1 so as to improve the virus titer. The strain is cultured in a PK-15 NPM1 cell to obtain a high-titer virus, then the high-titer virus is inactivated, and an ISA 201 VG adjuvant is used for preparing the inactivated vaccine. Compared with mineral oil adjuvants used in current commercial vaccines, the inactivated vaccine is easier to inject and absorb and low in side reaction; compared with the prior art, the method has the advantages that the time for generating the specific antibody is early, and the antibody level is high, so that the immune response window period is shortened, the replication of PCV2 in an animal body can be more effectively resisted, the pathological change of the animal body caused by PCV is relieved, the body can be effectively protected by the antibody in the early stage of immunization, and a better protection effect is provided for resisting PCV2d subtype infection by swinery.

The invention discloses a multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) reagent for synchronously detecting five DNA (Deoxyribose Nucleic Acid) viruses related to infertility. The multiplex fluorescent quantitative PCR reagent comprises specific primers and probes aiming at a simian vacuolar virus 40, a molluscum contagiosum virus, a Merkel cell polyoma virus, a BK virus and a torque teno virus; according to the method, the five viruses can be synchronously detected in a single reaction tube, meanwhile, the load of the viruses can be quantified, the reagent has the advantages of being high in specificity and sensitivity, high in repeatability and reproducibility, easy to operate, short in consumed time and the like, and when large-scale clinical screening application is carried out, the reagent has the advantages of being high in specificity and sensitivity, good in repeatability and reproducibility and the like. The detection efficiency of the five viruses can be greatly improved, the detection cost can be reduced, and the detection result can provide important reference for the formulation of an individualized treatment scheme, so that the application has important value and significance for clinical rapid screening and diagnosis and improvement of the treatment effect.

The invention relates to the technical field of veterinary biological product production, and discloses a suspension serum-free PK cell based on a composite synergist and a method for culturing porcine circovirus by using the suspension serum-free PK cell, a high-quality suspension cell strain is obtained by constructing an adherent PK cell suspension technical system of gradient domestication-monoclonal screening-performance verification; according to the method, a composite synergist composed of IGF1R, L-selenomethionine, polyvinylpyrrolidone (PVP) and a Cu / Mn compound is innovatively introduced, and specific proliferation parameter optimization of PCV2 and PCV3 strains is combined, so that efficient and stable culture of PCV2 / PCV3 is realized, and the virus titer and production efficiency are remarkably improved.