7 results about "Cyclovirus" patented technology

The invention relates to the technical field of molecular biological detection. The invention provides a specific primer group and a specific probe for simultaneously detecting and distinguishing six torque teno virus subtypes. The six torque teno virus subtypes comprise a TTV-29 subtype, a TTV-16 subtype, a TTV-15 subtype, a TTV-24 subtype, a TTV-1 subtype and a TTV-19 subtype. The specific primer group and the specific probe are used for qPCR amplification, the six most common torque teno virus subtypes in HSCT patients can be detected and distinguished at the same time, the detection speed is high, the specificity is high, and the problems that in the prior art, the detection efficiency is low, and the typing capacity is insufficient are effectively solved; meanwhile, the detection method is simple, short in detection time, low in cost, high in accuracy and easier to accept by patients.

The invention relates to the technical field of medicine, in particular to a porcine circovirus type 3 Cap recombinant protein and a matched detection method and application thereof.According to the porcine circovirus type 3 Cap recombinant protein, efficient and high-quality preparation is achieved through gene optimization and expression process improvement, Escherichia coli rare codons in ORF2 genes are replaced with GeneOptimizer software, an unfavorable structure rich in arginine at the N end is removed, and the recombinant protein is obtained. The mRNA stability and the translation efficiency are obviously improved; through IPTG induction condition optimization and'inclusion body treatment-Ni-NTA affinity chromatography-dialysis renaturation 'purification processes, the final protein purity exceeds 95%, a high-quality material basis is provided for subsequent detection, the matched indirect ELISA detection method has the core characteristics of'accuracy, high efficiency and stability', detection can be completed within 2-3 h, synchronous analysis of large-batch samples is supported, and the detection efficiency is greatly improved.

The invention relates to the technical field of medical examination and clinical prediction models. The invention provides an application of a system for detecting torque teno virus load in preparation of a product for predicting or assisting in predicting the condition of a hematopoietic stem cell transplantation patient, and a risk assessment model for predicting the outcome of HSCT patient infection, graft versus host disease, relapse, non-relapse death and the like is established by detecting the dynamic change of torque teno virus load. Therefore, early warning and risk layering of the illness state of the patient before clinical symptoms appear are realized, and the accuracy of patient management and treatment is improved.

The invention provides a stirring type reactor culture process of a porcine circovirus type 2 recombinant baculovirus, which comprises the following steps: preparing a seed virus of the porcine circovirus type 2 recombinant baculovirus by using an insect cell Sf9 full-suspension culture process, and inoculating an insect cell High Five by using the seed virus prepared by using an Sf9 cell to perform antigen expression of the porcine circovirus type 2 recombinant baculovirus. A full-suspension culture mode is utilized, so that the labor intensity is reduced, the production cost is reduced, and pollution is not easily caused; 2) the risk of porcine virus pollution can be reduced by using insect cells and a baculovirus expression system; and 3) the characteristics (Sf9 and High Five) of different insect cells are simultaneously utilized to respectively carry out seed virus preparation and antigen expression, so that higher virus titer can be obtained, the virus titer is greater than or equal to 7.50 Log (TCID50 / mL), higher recombinant protein expression is obtained, and the protein expression quantity is greater than or equal to 150 mu g / mL.

The application discloses a kind of graft injury multi-index combined noninvasive detection kit, by optimized multiplex real-time fluorescent quantitative polymerase chain reaction technology, realize the quantitative detection of six categories with closely related DNA virus after transplantation Complications, the present application relates to the field of in vitro detection technology of clinical sample.The graft injury multi-index combined noninvasive detection kit, the present application first realizes the synchronous detection of BK virus, JC virus, B19 virus, CMV virus, adenovirus and six kinds of closely related viruses of circovirus (TTV) with graft injury.Test data see example 7, from the detection results of 100 clinical kidney transplant patients, it can be seen that the kit of the present application can simultaneously accurately detect the load level of six viruses, to provide comprehensive infection risk assessment for clinicians.Compared with the prior art single virus detection method, the present application avoids the cumbersome process of multiple sampling and detection, significantly improves the clinical work efficiency.

The invention belongs to the field of immunology and genetic engineering, and particularly relates to a preparation method and application of a porcine circovirus 2d subtype strain inactivated vaccine. The PCV2d subtype strain obtained through separation is proliferated in a PK-15 cell of overexpressed NPM1 so as to improve the virus titer. The strain is cultured in a PK-15 NPM1 cell to obtain a high-titer virus, then the high-titer virus is inactivated, and an ISA 201 VG adjuvant is used for preparing the inactivated vaccine. Compared with mineral oil adjuvants used in current commercial vaccines, the inactivated vaccine is easier to inject and absorb and low in side reaction; compared with the prior art, the method has the advantages that the time for generating the specific antibody is early, and the antibody level is high, so that the immune response window period is shortened, the replication of PCV2 in an animal body can be more effectively resisted, the pathological change of the animal body caused by PCV is relieved, the body can be effectively protected by the antibody in the early stage of immunization, and a better protection effect is provided for resisting PCV2d subtype infection by swinery.

The invention relates to the technical field of veterinary biological product production, and discloses a suspension serum-free PK cell based on a composite synergist and a method for culturing porcine circovirus by using the suspension serum-free PK cell, a high-quality suspension cell strain is obtained by constructing an adherent PK cell suspension technical system of gradient domestication-monoclonal screening-performance verification; according to the method, a composite synergist composed of IGF1R, L-selenomethionine, polyvinylpyrrolidone (PVP) and a Cu / Mn compound is innovatively introduced, and specific proliferation parameter optimization of PCV2 and PCV3 strains is combined, so that efficient and stable culture of PCV2 / PCV3 is realized, and the virus titer and production efficiency are remarkably improved.