354results about How to "Genetically stable" patented technology

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g/l.

The invention relates to a method for breeding a novel bee species by breeding a hybrid of east bees and west bees, comprising introducing a west bee brood comb going to come out cells into a swarm of east bees preparing for laying eggs, to make the number of east worker bees equal to that of west worker bees, and obtaning a mixed swarm; the east queen bee laying eggs on empty combs, moving out the east queen bee to make the swarm without a queen, after the first generation east worker bees have got out cells; putting in a queen cell frame 20-22 hours later, breeding a queen with four-day old east bee grubs of same swarm to get a novel east queen bee; and hybridizing said queen as mother with west male bee as father by artificial insemination or natural copulation to obtain a hybrid thereof. The method breaks the reproductive isolation between two different species, and the hybrid obtained thereby has a remarkble heterosis.

The invention relates to a preparation method of Muscovy duck parvo novel vaccines, which comprises the following steps of: using Muscovy duck parvovirus attenuated virus P1 strain (MPV-P1 strain with a preservation number of CCTCC NO:V201013) and Muscovy duck origin goose parvovirus attenuated virus D strain ( GPV-D strain with a preservation number of CCTCC NO:V201014) as seed viruses; proliferating viruses by applying the muscovy duck embryone fibroblast spinner culture technology to obtain cell toxic liquids; mixing the cell toxic liquids according to the proper proportion and freezing a dry protective agent to freeze and dry to research safe and effective Muscovy duck parvo novel vaccines. The young Muscovy ducks are inoculated once so as to prevent and control the Muscovy duck parvovirus infection and the Muscovy duck gosling blast dieases at the same time, thereby the stress reaction of the Muscovy ducks caused by immunization for many times is solved. The invention is suitable for scale production under the GMP (Good Manufacturing Practices) condition, and saves the production cost of the vaccines.

The invention discloses debaryomyces hansenii as well as a fermentation fluid, a mixed fermentation fluid and application thereof to secondary fermentation of vegetables, wherein the debaryomyces hansenii is (Debaryomyces hansenii) W08 with preservation number of CGMCC No.5770. The invention also discloses a debaryomyces hansenii fermentation fluid which is obtained by inoculating the debaryomyces hansenii in a PDA fluid medium to culture. The invention also discloses a mixed fluid which comprises the debaryomyces hansenii fermentation fluid, a lactobacillus plantarum fermentation fluid and a leuconostoc mesenteroides fermentation fluid; the mixed fermentation fluid is applied to the secondary fermentation of vegetables, and has the effects of forming special micro-ecological environment and accelerating the forming of more substances with favorable flavor of vegetables to improve the edibility.

The invention relates to a slaughter type high-quality chilling fresh chicken breeding method. The breeding method includes: selecting recessive white-feather chickens (B line) having green shins and yellow skins as first male parents, and selecting large cockscomb high yield chickens (C line) having yellow shins and yellow skins as first female parents; hybridizing the B line and the C line to acquire parental generations (BC system) to be as final female parents according to the heredity principle that a green shin gene is sex-linked and an autosome controls a skin color gene, wherein parental chickens can be distinguished through the shin color, the shins of the female chickens are green, and the shins of the male chickens are yellow. using yellow-feather fast-growing chickens (A line) having green shins, yellow skins, and large cockscombs as final male parents, producing commodity chickens (ABC) by A line male X (B line male X C line female) female mating hybrid, wherein commodity male and female chickens have green shins, yellow skins, large cockscombs, and wide chests. The commodity chickens can be sold at the age of 63-65 days, the average weight of the female chickens is 1.6-1.65 Kg, the average weight of the male chickens is 1.8-1.85 Kg, the average uniformity can reach more than 96%; the dressing percentage, the whole net carcass rate, and the chest muscle rate can exceed 85%, 65%, and 18%, and the carcass character has significant advantages.

The invention relates to a porcine reproductive and respiratory syndrome virus chimeric recombinant vaccine strain and a method for producing living vaccine with same; latter half part of GP2 gene and GP3-GP5 gene of the porcine reproductive and respiratory syndrome virus (PRRSV) classic virus PTK strain, M ferritin, N ferritin and spacer sequences thereof and all nucleotide sequences of 3' non-coding region are all deleted by using reverse genetic manipulation and gene combination technology, then replaced with corresponding gene of Chinese highly pathogenic porcine reproductive and respiratory syndrome variant strain and are recombined to be the chimeric recombinant vaccine strain (PC strain) with the PRRSV classic strain and variant strain genes simultaneously, the chimeric recombinant vaccine PC strain is used to produce the living vaccine which has low toxicity, ensures no returning of strong toxicity within 5 generations of returning swine and the heritability is stable; the invention has good immunogenicity, and can prevent the porcine reproductive and respiratory syndrome virus classic strain and highly pathogenic variant strain from being infected simultaneously.

The invention discloses a method for molecular inspection of cowpea variety based on analysis of genome RAPD. Extracting DNA of the available genome cowpea varieties, and generating separately polyase chain reaction to prepare the random augmentative polymorphic DNA product, then processing electrophoretic separation to obtain random augmentative polymorphic DNA standard atlas; analyzing the inspection-required cowpea variety following said steps to get the random augmentative polymorphic DNA atlas, comparing and deciding it with standard atlas, the inspection result is obtained. With the invention, it can definitely decide the different varieties of cowpea, and realize precise and rapid inspection of variety truth and variety purity, providing reliable technique of variety inspection, market control of seed, variety protection, quality control of pre-sowing seed, stock breeding and so on.

First generation adenoviral vectors and-recombinant adenovirus-based HIV vaccines which contain HIV-1 gag, HIV-1 pol and/or HIV-1 nef polynucleotide pharmaceutical products, and biologically relevant modifications thereof are described. The adenovirus vaccines, when directly introduced into living vertebrate tissue, express the relevant proteins, inducing a cellular immune response which specifically recognizes HIV-1. The exemplified polynucleotides of the present invention are synthetic DNA molecules encoding HIV-1 Gag, HIV-1 Pol, HIV-1 Nef, and derivatives thereof. The adenoviral vaccines of the present invention, alone or in combination, will offer a prophylactic advantage to previously uninfected individuals and/or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of HIV-1 infection.

The invention belongs to the field of molecular genetics, and relates to molecular marker of heat stress resistant breeding of a Hostein cattle and application thereof. The molecular marker is SEQ ID NO.3. The molecular marker can be used to assistantly breed the Hostein cattle with the characteristic of heat stress resistance and higher milk yield.

The invention discloses a caproic acid fermented strain and a caproic acid extracting method. The caproic acid fermented strain is js-22-03, js-22-07 and js-22-12. Bred bacterial strains are good in passage stability, and the yield of the bred bacterial strains is increased to 1.90 g/100 mL from 1.20 g/100 mL compared with an original bacterial strain. The high-yield bacterial strains js-22-03, js-22-07 and js-22-12 are obtained through culture and screening, and the advantages of being short in fermentation time, stable in genetic property, high in yield and the like are achieved. The fermentation time is shortened to be within 10 days, and the caproic acid yield is increased to 1.90 g/100 mL from original 1.20 g/100 mL.

The invention relates to construction of saccharomyces cerevisiae engineering bacteria generating beta-phenethyl alcohol, a strain and application thereof, and belongs to the technical field of bioengineering and production of food spices. The prepared beta-phenethyl alcohol spice is a natural spice and can be used for preparing a plurality of food essences, and therefore, the beta-phenethyl alcohol can be widely applied to industries such as food, medicine and daily use chemicals. Engineering bacterial for coupling overexpression of an aminotransferase gene (ARO8) and decarboxylase gene (ARO10) in the anabolic way of beta-phenethyl are designed and constructed with saccharomycescerevisiae S288 as an original strain; gene copy and expression of the two key enzymes in the main synthetic way are enhanced; the bred engineering bacteria (transformants) are passaged a plurality of times and stable in inheritance; the metabolic flux and the yield of beta-phenethyl are obviously improved. With L-phenylalanine as a major raw material, the engineering bacteria are used for fermenting and converting the raw material into the beta-phenethyl alcohol spice which is single in configuration and chirality and high in fragrance quality, and therefore, a new idea and technical support are provided for preparation of the natural beta-phenethyl alcohol spice.

The invention relates to a VII type Newcastle disease virus L-gene mutation attenuated vaccine strain produced by using a reverse genetic manipulation technology and a preparation method of the VII type Newcastle disease virus L-gene mutation attenuated vaccine strain, and belongs to the field of biological products and biological technologies. Toxicity of the VII type Newcastle disease virus L-gene mutation attenuated vaccine strain is weakened obviously, and at the same time, the vaccine strain on a chick embryo has high titer, stable heritability and high compatibility to an antigen of a prevalent virus, is suitable for large-scale production of vaccines, and can be used for producing the vaccines. Compared with conventional vaccines (gene I and gene II types), the VII type Newcastle disease virus L-gene mutation attenuated vaccine strain has a wide application prospect in control of incidence and prevalence of the Newcastle diseases.

The invention discloses a breeding method for local chicken variety resource protection and utilization, and belongs to the technical field of poultry breeding. According to the method, the local chicken variety resources are not manually selected, and the inherent characteristics of the original variety keep unchanged. During the utilization in each year, 12-week old cocks with the average body weight being 95 percent to 107 percent are selected and remained in a seed reserving group, and are subjected to hybrizing matching with cultured high-breeding-property or fast-growth new strains (matched strains), the appearance characteristics of the original local chickens are reserved, the egg laying performance/growth speed of the local variety is improved/accelerated by 15 to 20 percent. The method provided by the invention has the advantages that the original local variety is used as the strain for participating in the matching, the damage of current variety breeding or pure hybridization to resources in the past local chicken resource development and utilization is avoided, and the duplex effects of protection and utilization are achieved.

The invention aims at providing a porcine pseudorabies virus vaccine. The porcine pseudorabies virus vaccine comprises an antigen and a protective agent, wherein the antigen contains an attenuated virus strain which is prepared after deleting virulence genes by a porcine pseudorabies virus strain with the collection number of CGMCC No. 10266. The prepared vaccine can effectively prevent porcine pseudorabies; furthermore, because the porcine pseudorabies virus as the antigen is a gene-deleted strain, by continuous passage of horizontally transmitted infections in mouse bodies, no virulence reversion occurs, and the genetic stability is realized, thereby being in line with the standard of having no virulence reversion in the porcine pseudorabies virus deleted vaccine strain; and the prepared vaccine can provide effective immune protection, and has great commercialization development prospects.

A fast reproduction method of Anzu flower by inducing somatic cells includes such steps as picking up the young stem of Anzu flower, cutting by 3-7 mm in length, inoculating in the inducing culture meidum under aseptic condition, culturing at 25-27 deg.C in dark condition for 40-50 days to induce embryo of somatic cell, and culturing at 25-27 deg.C under 2000 LX (12-16 hr per day) for 50-60 days. Its advantage is fast speed.

The present invention discloses a breeding method for a white-feather intergenerically hybridized duck female parent. As an intergenerically hybridized feather color genetic mechanism is relatively complex, and selective advance by using a simple offspring phenotype is extremely slow, the invention creates a selecting method. By adopting a female parent offspring measuring method, by virtue of an artificial insemination technology and integrating an individual selection method and a family selection method, enclosed breeding and offspring production are performed for seed selection of about 5 generations, so that the white feather rate (the white feather area reaches over 95%) of intergenerically hybridized offsprings is significantly improved, and the genetic stability is good.

The invention discloses a schizochytrium limacinum strain as well as a building method and application thereof. The strain disclosed by the invention is classified and named as schizochytrium sp. HX-RS, and the preservation number of the schizochytrium sp. HX-RS is CCTCC NO: M2017046. According to the strain, an acyltransferase functional domain originating from shewanella oneidensis PKS enzyme, instead of an acyltransferase functional domain originating from schizochytrium sp. PKS enzyme, is adopted; the strain is obtained through flat panel screening and acclimation screening with high rotation speed and low temperature. The strain can be used for co-producing DHA and EPA; the contents of the EPA and the DHA obtained by carrying out culture fermentation of the strain respectively reach 4.68% and 51.3%, and compared with an original strain, the contents of the EPA and the DHA are respectively increased by 69.9% and 14.3%; moreover, the fermentation production efficiency is high and the cost is relatively low.

The invention provides an escherichia coli recombination strain T002 for producing levodopa as well as a construction method and application thereof. According to the escherichia coli recombination strain, expression of 3-dehydrogenase shikimate dehydrogenase (aroE) is up regulated to enhance enzyme activity of 3-dehydrogenation shikimate dehydrogenase, and the levodopa can be further produced. The strain can produce the levodopa by utilizing glucose inorganic salt culture medium production, culture medium cost is reduced, and production cost of the levodopa is reduced. Furthermore, the recombination strain has the advantages of no plasmid and stable hereditary.

The present invention discloses a Tricholoma matsutake sing mutagenesis strain and a breeding method thereof. According to the present invention, a new high-yield Tricholoma matsutake sing strain is obtained through chemical mutagenesis screening separation, wherein the preservation number is CCTCC N0:2014671, the mycelium dry weight is increased by 69.68% compared with the original strain, the adenosine content is increased by 33.96%, the polysaccharide content is increased by 75.72%, the protein content is increased by 35.17%, and the mannitol content is increased by 52.8%; and after subculturing is performed on the new Tricholoma matsutake sing strain more than or equal to 10 times, no degeneration is generated, and the genetic stability is provided. The present invention further discloses an optimal fermentation culture medium of the Tricholoma matsutake sing mutagenesis strain.