12results about How to "Genetically stable" patented technology

The invention belongs to the technical field of biology, and discloses a construction method of a swine cholera attenuated salmonella recombinant strain capable of simultaneously expressing a haemophilus parasuis outer membrane protein GAPDH gene and an Omp26 gene, a plasmid connected with the haemophilus parasuis GAPDH gene and the Omp26 gene is adopted to transfect a swine cholera salmonella asd gene deleted strain, screening is carried out, and screening is carried out to obtain the swine cholera attenuated salmonella recombinant strain capable of simultaneously expressing the haemophilus parasuis outer membrane protein GAPDH gene and the Omp26 gene. The recombinant attenuated salmonella choleraesuis expressing the haemophilus parasuis GAPDH gene and the Omp26 gene is obtained. The strain can simultaneously express a haemophilus parasuis outer membrane protein GAPDH gene and an Omp26 gene, and compared with a strain which singly expresses Omp26, the strain has the advantage that the immune protection effect is obviously improved. Meanwhile, the invention also provides a construction method and application of the strain.

The invention discloses geranyl and geranyl diphosphate synthetase for high yield of beta-carotene and application of the geranyl and geranyl diphosphate synthetase. Specifically, the invention discloses a method for producing beta-carotene, and the method comprises the following steps: overexpressing a CrtE gene, a CarRP gene and a CarB gene in a chassis strain; wherein the CrtE gene is a CrtE gene from Sulfolobus sp. And / or Sulfolobus sp. Acidophilus (Sulfolobus sp. Acidophilus), and the CrtE gene is a CrtE gene from Sulfolobus sp. Acidophilus (Sulfolobus sp. Acidophilus) and / or Sulfolobus sp. Acidophilus. According to the method, the lycopene substrate inhibition effect can be effectively relieved, and the yield of beta-carotene in a chassis strain is effectively increased.

This invention belongs to the field of biological breeding technology, specifically involving SlCSLA2 Application of genes in improving the storage and transportability of tomato fruit. This invention addresses the industry problem of severe storage and transport losses in tomatoes due to excessive softening after harvest. For the first time, it utilizes CRISPR / Cas9 gene editing technology to knock out the cell wall hemicellulose (mannan) synthase gene. SlCSLA2 This invention has led to the creation of a tomato mutant with significantly enhanced fruit firmness. The firmness of the entire fruit during the red-ripe stage is 35%–42% higher than that of the wild type, and the firmness can be maintained for about a week after harvest, effectively delaying the softening process and significantly improving storage and transportability. This invention provides new gene targets and technical approaches for breeding tomato varieties with improved storage and transportability.

The invention discloses a method for inducing mature embryos of Siberian apricot to breed seedlings, comprising the following steps: explant disinfection: disinfecting apricot kernels with seed shells removed and peeling off the seed coat to obtain mature embryos; sterile seedling induction: culturing mature embryos in sterile seedling induction medium to obtain sterile seedlings; sterile seedling proliferation: culturing sterile seedlings in sterile seedling proliferation medium to obtain sterile seedlings and strong seedlings; inducing callus: culturing rectangular leaves cut from sterile seedlings and strong seedlings in callus induction medium to obtain callus; differentiating cluster buds: transferring callus to cluster bud differentiation medium to obtain cluster buds; cluster bud subculture proliferation: culturing cluster buds in cluster bud subculture medium after cutting to obtain cluster bud strong seedlings; cluster bud rooting: cutting cluster buds into single buds and transferring them to rooting medium for rooting culture. The invention can solve the technical problems of long breeding cycle, low survival rate and poor breeding stability of Siberian apricot.

The invention relates to the technical field of animal breeding. The invention provides a breeding method of a new variety of meat sheep, which comprises the following steps: (1) taking Tibetan sheep as a male parent and small tailed han sheep as a female parent, and hybridizing to obtain an F1 generation; (2) taking Tibetan sheep as a male parent, selecting high-quality ewes from the F1 generation as a female parent, and hybridizing to obtain an F2 generation; (3) selecting high-quality ewes from the F2 generation as female parents by taking the Tricel sheep as male parents, and hybridizing to obtain an F3 generation; and (4) selecting high-quality ewes and high-quality rams from the generation F3, and carrying out cross-crossing fixation. According to the technical scheme, a new variety which is stable in hereditary performance, good in ketone body quality, excellent in meat quality and high in growth speed can be obtained, and the variety requirement of mutton sheep industry development is met.

The invention relates to the field of molecular biology, in particular to an Indel marker linked with a flowering cabbage branching gene Brbc1 and application of the Indel marker, two Indel markers A0729 and A0749 are located on the two sides of Brbc1 respectively, and two pairs of insertion-deletion polymorphism Indel markers linked with the Brbc1 gene are obtained through development and can be applied to molecular identification of the number of flowering cabbage breeding offspring branches and improvement of breeding efficiency. The Indel marker is developed by exploring markers closely linked with flowering cabbage branch related genes, the genetic effect of the Indel marker related to the flowering cabbage branch characters is obvious and stable, and the requirements of molecular breeding can be met.

This invention discloses a strain of *Streptomyces luteoverticillatus* and its application in controlling tomato leaf mold. *Streptomyces luteoverticillatus* RZ0102, which exhibits strong inhibitory effects against tomato leaf mold, was screened from river mud at the confluence of the Futong River and the sea in Rizhao. The strain's preservation number is CGMCC NO.15219. Experiments show that this strain possesses genetic stability, and its fermentation supernatant exhibits excellent thermal stability, pH stability, and UV irradiation stability. Both strain RZ0102 and its metabolites can effectively inhibit the growth of tomato leaf mold pathogens.

The application discloses a kind of Vibration Mountain beard toad hind leg / toe tissue cells and its quick acquisition method and application.The method comprises: the body surface disinfection of Vibration Mountain beard individual, cut and take hind leg or toe tissue, and put back after wound disinfection;After tissue is soaked in 75% alcohol and washed with PBS containing penicillin, streptomycin and amphotericin B, it is temporarily stored in 4 ℃ temporary storage solution;After tissue is cut, it is sequentially digested with trypsin and type I collagenase;In the optimized low permeability medium, primary culture is carried out at 25-27 ℃;When cell confluence reaches 60%-70%, it is passaged using trypsin-EDTA digestion;Freezing and recovery.The application realizes the non-lethal cell of Vibration Mountain beard for the first time Quick acquisition, cell first time migration time is only 3 days, grow into monolayer only 12-17 days, pollution rate is reduced to 5.6%, recovery survival rate reaches 76.19%, and provides efficient and reliable technical support for endangered amphibian germplasm resource preservation and biological research.

The invention discloses streptomyces luteus and application of the streptomyces luteus in prevention and treatment of tomato leaf mold. According to the invention, streptomyces luteoverti RZ0102 with a relatively strong inhibition effect on tomato leaf mold is screened from river mud at the junction of the River and the sea, and the preservation number of the streptomyces luteoverti RZ0102 is CGMCC (China General Microbiological Culture Collection Center) NO.15219. The streptomyces luteoverti RZ0102 has the advantages that the streptomyces luteoverti RZ0102 has a relatively strong inhibition effect on the tomato leaf mold; experiments show that the strain has genetic stability in function, and fermentation supernate of the strain has excellent thermal stability, acid-base stability and ultraviolet irradiation stability; the strain RZ0102 and metabolites thereof can efficiently inhibit the growth of the tomato leaf mold bacteria.

The invention discloses an artificial propagation-hybridization combined breeding method for meat goats, and relates to the technical field of animal breeding, and the method comprises the following specific steps: selecting a goat variety with good meat performance as a female parent, and selecting a goat variety with large physique as a male parent; the method comprises the following steps: performing in-vitro culture on female parent oocytes to obtain mature oocytes containing first polar bodies, screening high-activity sperms by adopting a temperature gradient screening device, performing in-vitro fertilization on the mature oocytes and the high-activity sperms, culturing the mature oocytes and the high-activity sperms to a hybrid blastocyst form, transplanting the mature oocytes and the high-activity sperms into a female body, and developing the mature oocytes and the high-activity sperms until the mature oocytes and the high-activity sperms are naturally delivered to obtain a hybrid F1 generation; backcrossing the F1 generation with the female parent, backcrossing the backcrossed offspring with the female parent for multiple times until a breeding target is met, and carrying out cross-crossing fixation to obtain a new variety / line with excellent characters; the sperm screening efficiency is improved by utilizing the screening device, and the quality of the screened sperms is ensured, so that the fertilization efficiency and the embryo survival rate are remarkably improved.

This invention relates to the fields of molecular biology and plant molecular marker breeding technology, and discloses an Indel marker co-segregating with the disease resistance trait in pepper and its application. The Indel marker is located at 29,449,971-29,449,991 bp on chromosome 5 of the pepper cv.CM334 genome; compared with the disease-susceptible pepper, the disease-resistant pepper exhibits a deletion of Indel 73. The marker provided by this invention is tightly linked to the disease resistance gene in pepper, exhibits stable localization, and is suitable for molecular breeding; it allows for the detection of the disease resistance genotype in pepper under laboratory conditions, without environmental limitations. Using this marker, rapid disease resistance testing of individual pepper plants can be performed at any growth stage without affecting their normal growth; the molecular marker of this invention can lay the foundation for cloning the disease resistance gene in pepper and studying the molecular mechanism of disease resistance in pepper, and has application value in the breeding of new varieties.

The invention provides a compound microbial agent as well as a preparation method and an application method thereof, aiming at the problems of environmental pollution, drug resistance, unstable biocontrol effect of a single strain and the like which are easily caused by existing chemical control, and belongs to the technical field of microbial preparations and biological control of plant diseases. The microbial agent is composed of bacillus aryabhattai MES85801 (CGMCC No.25404), bacillus velezensis MES861 (CGMCC No.16857), bacillus subtilis MES810 (CGMCC No.14514) and bacillus amyloliquefaciens MES81165, liquid fermentation culture is performed till the spore formation rate is larger than or equal to 90%, glycerin, skim milk powder, sodium alginate and a PVP composite stabilizer are added, spray drying is performed to prepare a powder preparation, and the effective viable count of the product is larger than or equal to 1.0 * 10 CFU / g. The complex microbial inoculant has a remarkable antagonistic effect on pathogenic bacteria of peach tree canker, has the functions of promoting growth, improving quality and increasing yield, and is suitable for the sustainable development requirement of green agriculture.