5results about How to "Genetically stable" patented technology

This invention belongs to the field of biological breeding technology, specifically involving SlCSLA2 Application of genes in improving the storage and transportability of tomato fruit. This invention addresses the industry problem of severe storage and transport losses in tomatoes due to excessive softening after harvest. For the first time, it utilizes CRISPR / Cas9 gene editing technology to knock out the cell wall hemicellulose (mannan) synthase gene. SlCSLA2 This invention has led to the creation of a tomato mutant with significantly enhanced fruit firmness. The firmness of the entire fruit during the red-ripe stage is 35%–42% higher than that of the wild type, and the firmness can be maintained for about a week after harvest, effectively delaying the softening process and significantly improving storage and transportability. This invention provides new gene targets and technical approaches for breeding tomato varieties with improved storage and transportability.

This invention discloses a strain of *Streptomyces luteoverticillatus* and its application in controlling tomato leaf mold. *Streptomyces luteoverticillatus* RZ0102, which exhibits strong inhibitory effects against tomato leaf mold, was screened from river mud at the confluence of the Futong River and the sea in Rizhao. The strain's preservation number is CGMCC NO.15219. Experiments show that this strain possesses genetic stability, and its fermentation supernatant exhibits excellent thermal stability, pH stability, and UV irradiation stability. Both strain RZ0102 and its metabolites can effectively inhibit the growth of tomato leaf mold pathogens.

The application discloses a kind of Vibration Mountain beard toad hind leg / toe tissue cells and its quick acquisition method and application.The method comprises: the body surface disinfection of Vibration Mountain beard individual, cut and take hind leg or toe tissue, and put back after wound disinfection;After tissue is soaked in 75% alcohol and washed with PBS containing penicillin, streptomycin and amphotericin B, it is temporarily stored in 4 ℃ temporary storage solution;After tissue is cut, it is sequentially digested with trypsin and type I collagenase;In the optimized low permeability medium, primary culture is carried out at 25-27 ℃;When cell confluence reaches 60%-70%, it is passaged using trypsin-EDTA digestion;Freezing and recovery.The application realizes the non-lethal cell of Vibration Mountain beard for the first time Quick acquisition, cell first time migration time is only 3 days, grow into monolayer only 12-17 days, pollution rate is reduced to 5.6%, recovery survival rate reaches 76.19%, and provides efficient and reliable technical support for endangered amphibian germplasm resource preservation and biological research.

This invention relates to the fields of molecular biology and plant molecular marker breeding technology, and discloses an Indel marker co-segregating with the disease resistance trait in pepper and its application. The Indel marker is located at 29,449,971-29,449,991 bp on chromosome 5 of the pepper cv.CM334 genome; compared with the disease-susceptible pepper, the disease-resistant pepper exhibits a deletion of Indel 73. The marker provided by this invention is tightly linked to the disease resistance gene in pepper, exhibits stable localization, and is suitable for molecular breeding; it allows for the detection of the disease resistance genotype in pepper under laboratory conditions, without environmental limitations. Using this marker, rapid disease resistance testing of individual pepper plants can be performed at any growth stage without affecting their normal growth; the molecular marker of this invention can lay the foundation for cloning the disease resistance gene in pepper and studying the molecular mechanism of disease resistance in pepper, and has application value in the breeding of new varieties.

The invention provides a compound microbial agent as well as a preparation method and an application method thereof, aiming at the problems of environmental pollution, drug resistance, unstable biocontrol effect of a single strain and the like which are easily caused by existing chemical control, and belongs to the technical field of microbial preparations and biological control of plant diseases. The microbial agent is composed of bacillus aryabhattai MES85801 (CGMCC No.25404), bacillus velezensis MES861 (CGMCC No.16857), bacillus subtilis MES810 (CGMCC No.14514) and bacillus amyloliquefaciens MES81165, liquid fermentation culture is performed till the spore formation rate is larger than or equal to 90%, glycerin, skim milk powder, sodium alginate and a PVP composite stabilizer are added, spray drying is performed to prepare a powder preparation, and the effective viable count of the product is larger than or equal to 1.0 * 10 CFU / g. The complex microbial inoculant has a remarkable antagonistic effect on pathogenic bacteria of peach tree canker, has the functions of promoting growth, improving quality and increasing yield, and is suitable for the sustainable development requirement of green agriculture.