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32 results about "Reproductive isolation" patented technology
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The mechanisms of reproductive isolation are a collection of evolutionary mechanisms, behaviors and physiological processes critical for speciation. They prevent members of different species from producing offspring, or ensure that any offspring are sterile. These barriers maintain the integrity of a species by reducing gene flow between related species.
Seed producing method for obtaining hybrid advantage of crassostrea gigas
InactiveCN101642064AStrong disease resistanceImprove survival rateClimate change adaptationPisciculture and aquariaDiseaseCrassostrea
The invention relates to a seed producing method for obtaining the hybrid advantage of a crassostrea gigas, which is characterized by comprising the following steps: selecting Chinese and Korean crassostrea gigases as parent oysters to carry out temporary culture; ripening the Chinese and Korean crassostrea gigases synchronously; then hybridizing sperms and ovums of the Chinese and Korean crassostrea gigases by artificial insemination; and finally carrying out the incubation, the seed culture and the growth of the hybridized oyster. The invention cultures a crassostrea gigas hybridized seed with high disease resistance and survival rate and rapid growing speed by hybridizing two crassostrea gigas geographic populations with reproductive isolation; the production performance of the crassostrea gigas hybridized seed is obviously better than that of a Chinese locally inbred comparison population; and the method is simple and has favorable industrialized application prospect.
Owner:OCEAN UNIV OF CHINA
Method for establishing giant grouper sperm freezer and assisting grouper distant hybridization breeding
ActiveCN106417113ASolve the problem of the small number of male fishResolving Maturity Out of SyncClimate change adaptationDead animal preservationBiologyGeographic distribution
The invention discloses a method for establishing a giant grouper sperm freezer and assisting grouper distant hybridization breeding. The sperm freezer establishing method comprises the steps of preparing sperm diluent, preparing sperm cryopreservation liquid, freezing and storing sperms and establishing the sperm freezer. The grouper distant hybridization breeding method comprises the steps of establishing the giant grouper sperm freezer, establishing saladfish breeding groups, cultivating saladfish parent fish and performing reproductive regulation, performing artificially spawning breeding, fertilizing the frozen sperms and performing incubation, and cultivating fries. According to the method, the first giant grouper sperm freezer is established in China, reproductive isolation of the groupers caused by factors, such as geographical distribution, optimum temperature range and breeding time, is overcome, and distant hybridization breeding of the grouper groups is possible, so that distant hybridization breeding varieties are cultivated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Method for obtaining variety of Oplopanax elatus with heterosis
InactiveCN101278659AGood production traitsFast growthClimate change adaptationPisciculture and aquariaHeterosisUltraviolet radiation
A seed production method for obtaining trepang heterosis is characterized in that Chinese seed trepangs and Japanese seed trepangs are ripened synchronously firstly; then, artificial egg taking is processed under the conditions that the environment is dried in the shade and the temperature is heated and seawater is irradiated by ultraviolet radiation; the sperm and the egg of Chinese trepangs and Japanese trepangs are hybridized; finally, the hatch and the seed rearing of hybridized trepangs are processed by a normal method. The invention has the advantages as follows: Chinese trepangs and Japanese trepangs are ripened synchronously by temperature adjustment; two procreation-isolated and different geographical populations are hybridized to cultivate a hybridized trepang seedling with fast growth speed, strong stress resistance and high survival rate. The production character of the hybridized trepang seedling is obviously better than Chinese local selfing control population, which has very good industrialization application prospect.
Owner:OCEAN UNIV OF CHINA
Artificial hybridization breeding method for groupers high in growth speed
ActiveCN110521641ADelicious meatHigh nutritional valueClimate change adaptationPisciculture and aquariaArtificial inseminationCage culture
The invention relates to an artificial hybridization breeding method for groupers high in growth speed, and belongs to the technical field of aquaculture. The method comprises the steps of parent selection, cage culture of parent red-spotted groupers, industrial culture of parent epinephelus tukula, sexual transformation of the epinephelus tukula, freeze preservation of seminal fluid of the epinephelus tukula, spawning induction of the parent red-spotted groupers, artificial insemination and hatching of fertilized eggs. By using the sperm freeze preservation technology, an epinephelus tukula sperm bank is built, reproductive isolation between the two kinds of groupers is broken through, and a new excellent hybrid variety which is high in growth speed is obtained. The problem is solved thatmale parent epinephelus tukula produces fewer sperms, and consequently, production is hindered, the productivity is greatly improved, and grouper breeding work is facilitated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Maize reproductive isolation method
The invention discloses a maize reproductive isolation method. According to the invention, maize inter-variety reproductive isolation is realized through a maize hybridization unidirectional incompatibility gene. The maize hybridization unidirectional incompatibility gene is Gal-S or a gene having functions same with or similar to that of the Gal-S gene. The maize containing the Gal-S gene does not accept pollens of other maize which does not contain the gene. Even if different varieties are planted alternately, the maize is not influenced by pollens of other varieties. With the method, inherent defects of existing time reproductive isolation, space reproductive isolation and natural barrier reproductive isolation can be completely solved.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Construction method of Portunus trituberculatus fast-growth strain
ActiveCN101736074AImprove effectivenessAvoid blindnessMicrobiological testing/measurementPlant genotype modificationMicrosatelliteAquatic product
The invention relates to the field of aquatic product inheritance breeding, in particular to a construction method of Portunus trituberculatus fast-growth strain. The genome microsatellite of breeding materials are utilized to serve as molecular markers; according to the molecular marker, obtaining a group which has obvious reproductive isolation in hereditary constitution as a hybridization group, and carrying out hybridized combination to the hybridization group to obtain the Portunus trituberculatus fast-growth strain. The invention can utilize the species genetic resources to the utmost, which is favorable for more scientifically cultivating the Portunus trituberculatus fast-growth strain.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Hybrid breeding method of frozen sperms of little yellow croaker and large yellow croaker
InactiveCN110495410AOvercoming reproductive isolationArtificial hybridizationClimate change adaptationDead animal preservationSeawaterAquaculture
The invention discloses a hybrid breeding method of frozen sperms of small yellow croaker and large yellow croaker, and belongs to the technical field of fry breeding of aquaculture marine fish. The method specifically comprises the following steps that small yellow croaker sperms are collected, a sperm dilute containing an anti-freezing agent is added in the small yellow croaker sperms, cryopreservation is conducted, large yellow croaker ovums are collected, the small yellow croaker sperms are thawed, and the sperms and the ovums are subjected to artificial insemination. According to the method, the sperms with the survival rate being 60% after thawing are selected to be crossed with the large yellow croaker ovums, the fertilization rate reaches 67.3%, and the hatching rate is 86.8% or above. The method has the important significance for overcoming reproductive isolation, achieving artificial hybridization, cultivating new varieties, and achieving large-scale fry breeding.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Plant in-vivo somatic cell hybridization method
The invention relates to a plant in-vivo somatic cell hybridization method which comprises the following steps: selecting a female organ of a good variety of a plant type expected to be obtained and a somatic cell cluster of a reproduction unit as a hybridization female parent receptor, performing treatment before insemination to remove reproduction isolation, so that the hybridization female parent receptor is easy to fertilize; individuals, organs, tissues, cells, chromosomes or chromosome segments, DNA or DNA segments and genes of animals or plants, microorganisms and other organisms with the characters of the expected variety are selected as male parent donors, a somatic cell hybridization agent is added and stirred evenly, a sperm is obtained, the sperm is used for insemination of a receptor under mediation of the somatic cell hybridization agent, and the variety is obtained. And embryo rescue and seedling culture are carried out after the fertilization embryo is taken back. The bottleneck of distant hybridization is broken through, high-yield and high-quality varieties can be cultivated, and the method has wide application prospects.
Owner:深圳前海觉民科技有限公司
Hybrid seedlings-cultivating method between Chinese stichopus japonicus and Japan black stichopus japonicus
InactiveCN102106271AOvercome qualityOvercome speedClimate change adaptationPisciculture and aquariaChina seaPolysiphonia nigra
The application relates to a hybrid seedlings-cultivating method of the stichopus japonicus. The method comprises the steps of: firstly taking the stichopus japonicus in the China sea area and the black stichopus japonicus in the Japan northern sea area as the bred stichopus japonicus, and synchronously fertilizing the bred stichopus japonicus of the China stichopus japonicus and the bred stichopus japonicus of the Japan stichopus japonicus by controlling the cultivating water temperature and the bait feeding quantity of the bred stichopus japonicus in the process of artificial fertilizing and cultivating; artificially spawning and discharging sperm under the oxytocic condition of drying in the shade and warming, and respectively collecting the ovum from the Chinese stichopus japonicus and the sperm from the Japan black stichopus japonicus or the sperm from the Chinese stichopus japonicus and the ovum from the Japan black stichopus japonicus for the aritificial exomixis; and performing the incubation and the seedlings cultivation to the oosperm which is generated by hybridizing the two different geographical colonies which are reproductively isolated from each other according to the normal mode for cultivating the seedlings of the stichopus japonicus so as to cultivate the hybrid stichopus japonicus seedlings.
Owner:SHANDONG HAIYIBAO AQUATIC PROD
Haploid induction line genetic purity preservation method based on reproductive isolation characters
ActiveCN113557955AAvoid Inducing Performance EffectsSolve the problem of genetic purityMicrobiological testing/measurementPlant genotype modificationBiotechnologyWaxy corn
The invention discloses a haploid induction line genetic purity preservation method based on reproductive isolation characters. According to the method, a reproductive isolation character gene is applied to genetic purity preservation work of the induction line to form a haploid induction line material which only accepts self pollen and has a biological isolation function. The haploid induction line material with the biological isolation function is beneficial to avoiding the influence of external pollen on the induction performance of the induction line in commercial DH breeding application, solves the genetic purity preservation problem in the induction line breeding process and provides a feasible scheme for solving the problem of purity preservation work of a key induction line material in the haploid breeding process, the breeding workload of the induction line can be greatly reduced, and the material purity of the induction line can be ensured; and a new thought is provided for breeding work of pop corn and part of sweet-waxy corn materials, and popularization and application of a haploid breeding technology are promoted.
Owner:CHINA AGRI UNIV
Method for obtaining male pronucleus by using xenogeneic animal oocytes as medium
ActiveCN110684720AIncrease formation rateIncrease profitNew breed animal cellsEmbryonic cellsAnimal scienceMale pronucleus
The invention relates to the technical field of embryo engineering, in particular to a method for obtaining male pronucleus by using xenogeneic animal oocytes as a medium. The method comprises the following steps of step 1, obtaining mature oocytes of animals A, with a first polar body, and placing the obtained mature oocytes in external fertilization liquid droplets of animals B; step 2, injecting the sperms of the animals B into the liquid droplets containing the oocytes in the step 1 to obtain sperm and oveum mixed droplets; and step 3, placing the sperm and oveum mixed droplets obtained inthe step (2) in a CO2 incubator, and performing hatching until sperms of the animals B enter the cytoplasm of the oocytes to form male pronucleus, wherein the animals A and the animals B are xenogeneic animals. According to the method, a fertilization biology principle of reproductive isolation of uncrossed fertilization of the xenogeneic animals is used, an external fertilization method is adopted, the oocytes of the xenogeneic animals are used as a medium to obtain the male pronucleus of another animal, and the formation rate of the male pronucleus is as high as 17.8% or above.
Owner:武汉纽利特生物技术有限公司
Rice breeding method capable of preventing pollen drifting
The invention discloses a method of paddy rice breeding with the function of preventing pollen from flowing. The method comprises the following steps: a paddy rice reproductive isolation gene is introduced into the paddy rice so that cross-pollinated hybrid F1 cannot grow normally in a paddy field, thus achieving the purpose of preventing the pollen from flowing and ensuring purity of the paddy rice seed. The paddy rice reproductive isolation gene can be chosen from any one of the genes which can generate the following characteristics: hybrid weakness, hybrid lethality, hybrid collapse, hybrid sterility and hybrid depression.
Owner:CHINA NAT RICE RES INST
New method for mapping corn one-way outcross incompatibility gene
ActiveCN103352071AAvoid positioning deviationSave human effortMicrobiological testing/measurementGenotypeBiology
The invention belongs to the technical field of corn gene mapping, and particularly to a new method for mapping corn one-way outcross incompatibility gene. The conventional method for mapping qualitative character gene is constructing a backcrossing or self-crossing population, inferring a genotype of an individual through phenotypic identification, carrying out molecular marker detection, and adopting two point and three point tests to determine a position of the gene, wherein phenotypic identification is easily affected by human factors and environmental factors and the mapped population size is limited due to large workload when the conventional method is used to map the Ga gene. According to the present invention, based on the disadvantages of Ga gene mapping of the conventional method, a mapping method without phenotypic identification is provided, and the method is characterized in that the genotype of the utilized mapped population is known, the phenotype does not require identification, the large population can be constructed, and sufficient individuals can be found in the one generation so as to carry out fine mapping on the Ga gene; and an effective method for Ga gene mapping is provided, and application of the Ga gene to carry out reproduction separation on different types of corn on the production is promoted.
Owner:渤海粮仓南皮种业有限公司
Method for breaking self-incompatibility of apple to obtain filial generation
The invention relates to a method for breaking self-incompatibility of an apple to obtain a filial generation. The method comprises the following steps of: taking apple trees with good growth vigor and strong development as female parent plants, classifying flower buds in different open states, wherein flowers with buds expanding but being covered by sepals are defined as first age class flowers,flowers with the buds protruding out of the sepals are defined as second age class flowers, flowers with the buds breaking through the sepals but being not unfolded are defined as third age class flowers, flowers with the buds opening are defined as fourth age class flowers; selecting the second age class flowers and the third age class flowers to perform self-pollination. The invention provides aclassification method of flower age classes before apple flowers blooming overcomes the reproductive isolation caused by self-incompatibility to a certain extent and successfully obtains the filial generation by performing self-pollination on styles of different flower age classes before blooming. The method can be used for simply and conveniently obtaining an inbred filial generation of the apple which is difficult to obtain by a conventional pollination method; and an effective mean is provided for obtaining genetic materials of allele homozygosity in the apple breeding process.
Owner:CHINA AGRI UNIV
New method for mapping corn one-way outcross incompatibility gene
ActiveCN103352071BAvoid positioning deviationSave human effortMicrobiological testing/measurementGenotypeBiology
The invention belongs to the technical field of corn gene mapping, and particularly to a new method for mapping corn one-way outcross incompatibility gene. The conventional method for mapping qualitative character gene is constructing a backcrossing or self-crossing population, inferring a genotype of an individual through phenotypic identification, carrying out molecular marker detection, and adopting two point and three point tests to determine a position of the gene, wherein phenotypic identification is easily affected by human factors and environmental factors and the mapped population size is limited due to large workload when the conventional method is used to map the Ga gene. According to the present invention, based on the disadvantages of Ga gene mapping of the conventional method, a mapping method without phenotypic identification is provided, and the method is characterized in that the genotype of the utilized mapped population is known, the phenotype does not require identification, the large population can be constructed, and sufficient individuals can be found in the one generation so as to carry out fine mapping on the Ga gene; and an effective method for Ga gene mapping is provided, and application of the Ga gene to carry out reproduction separation on different types of corn on the production is promoted.
Owner:渤海粮仓南皮种业有限公司
Establishment of a sperm freezer for grouper serrata and method for assisting grouper distant hybrid breeding
ActiveCN106417113BGrow fastIncrease success rateClimate change adaptationDead animal preservationBiologyGeographic distribution
The invention discloses a method for establishing a giant grouper sperm freezer and assisting grouper distant hybridization breeding. The sperm freezer establishing method comprises the steps of preparing sperm diluent, preparing sperm cryopreservation liquid, freezing and storing sperms and establishing the sperm freezer. The grouper distant hybridization breeding method comprises the steps of establishing the giant grouper sperm freezer, establishing saladfish breeding groups, cultivating saladfish parent fish and performing reproductive regulation, performing artificially spawning breeding, fertilizing the frozen sperms and performing incubation, and cultivating fries. According to the method, the first giant grouper sperm freezer is established in China, reproductive isolation of the groupers caused by factors, such as geographical distribution, optimum temperature range and breeding time, is overcome, and distant hybridization breeding of the grouper groups is possible, so that distant hybridization breeding varieties are cultivated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Reproductive isolation control method for cycadopsida
InactiveCN101002542AImprove seed setting ratePromiscuity controlPlant genotype modificationAngiosperms/flowering plantsCycasPollination
A reproduction isolating and controlling method for cycas revolute-family plants includes such steps as determining the time when pollen is taken, taking pollen, determining the time when pollination is preformed, and artificial pollination.
Owner:SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
The Lrr-RLK receptor kinase ATMDIS1 and its use in breaking reproductive isolation between species
The invention discloses LRR-RLK receptor kinase AtMDIS1 having high expression in arabidopsis plant pollen and a method for breaking a close hybridization barrier through AtMDIS1. AtMDIS1 is expressed in species except arabidopsis through a transgenic means, gene exchange between different species can be promoted, and the hybrid sterility barrier in hybridization breeding is broken. Experiments prove that the method can successively improve arabidopsis ovule recognition efficiency of close species plant male gametophytes.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Construction method of Portunus trituberculatus fast-growth strain
ActiveCN101736074BImprove effectivenessAvoid blindnessMicrobiological testing/measurementPlant genotype modificationAquatic productMicrosatellite
The invention relates to the field of aquatic product inheritance breeding, in particular to a construction method of Portunus trituberculatus fast-growth strain. The genome microsatellite of breeding materials are utilized to serve as molecular markers; according to the molecular marker, obtaining a group which has obvious reproductive isolation in hereditary constitution as a hybridization group, and carrying out hybridized combination to the hybridization group to obtain the Portunus trituberculatus fast-growth strain. The invention can utilize the species genetic resources to the utmost, which is favorable for more scientifically cultivating the Portunus trituberculatus fast-growth strain.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Artificial interspecific hybridization method for ruditapes variegata and ruditapes philippinarum
ActiveCN103749365AImproved Breeding TraitsBreak through the reproductive isolation barrierClimate change adaptationPisciculture and aquariaInterspecific hybridizationHeterosis
The invention discloses a distant hybridization method for ruditapes, in particular to an artificial interspecific hybridization method for ruditapes variegata and ruditapes philippinarum. According to the artificial interspecific hybridization method, a group hybridization mode is adopted, highest-yield artificial interspecific hybridization between the ruditapes variegata and the ruditapes philippinarum is achieved in China, breeding season reproductive isolation obstacles existing between the ruditapes variegata and ruditapes philippinarum are broken through, precious medium materials used for researching little ruditapes genetic evolution and species formation are obtained, a new species with the significant hybrid vigour is obtained at the same time, the foundation is laid for the little ruditapes industry development and genetic improvement, precious materials are provided for little ruditapes genetic evolution and species formation, and the direction is offered to the cultivation of the new species. The artificial interspecific hybridization method for the ruditapes variegata and the ruditapes philippinarum can produce large-scale hybrids, and effectively improve the current cultivations of little ruditapes.
Owner:DALIAN OCEAN UNIV
Macrobracium rosenbergii spermatozoon gelatinases, encoding gene and application thereof
InactiveCN101368178AUnderstand the mechanism of fertilizationUnderstanding the Mechanisms of Reproductive IsolationHydrolasesFermentationHybrid systemProteinase activity
The invention relates to protease-(M.rosenbergii sperm gelatinase-like protease, SGP) with the hydrolyzed gelatin function, which is purified from the sperm crude extract of giant freshwater prawn (M.rosenbergii), and a coded gene and an application thereof. The studies of enzyme related with fertilization on sperms of different species are helpful to understanding the mechanism of crustacean fertilization and the mechanism of the production of reproductive isolation between different species. Based on the theories and the techniques, the relevant researchers can establish a good hybrid system, thus obtaining high yield and fast growth hybrid species.
Owner:ZHEJIANG UNIV
A method for improving the efficiency of ribbed loofah seed production by using flowering-delayed metamaterials
ActiveCN105724239BEasy to shapeHigh combination abilityPlant genotype modificationHybrid seedLuffa acutangula
The invention discloses a method for improving seed production efficiency of luffa acutangula roxb by utilizing a flowering delay specific material. The method comprises the following steps: introducing a flowering gene of luffa cylindrixa roem into the luffa acutangula roxb through distant hybridization, multi-generation backcrossing and selfing breeding and cultivating an excellent luffa acutangula roxb selfing line of which the flowering time is delayed for 0.5-1 hour, wherein the selfing line is excellent in comprehensive character and high in combining ability and simultaneously and effectively solves the problems of distantly-hybridized progeny fruit bitterness, hybrid dysgenesis and other reproductive isolations. The selfing line is taken as a female parent for hybrid seed production, so that the working time of removing male flowers of the female parent can be delayed in the seed production practice, therefore, the removal efficiency of the male flowers of the female parent is improved, and the purity of a hybrid is ensured; meanwhile, the effective working time of workers can be increased, the cost is lowered, and the working efficiency is improved.
Owner:肇庆全发农业发展有限公司
Method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority
PendingCN112106698AFast growthAndrogenic deficiencyClimate change adaptationPisciculture and aquariaAnimal scienceGermplasm
The invention discloses a method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority. The method comprises the following steps of (1), screening and cultivating parent fish groups, wherein the selection strength of epinephelus fuscoguttatus is 5%, and the selection strength of epinephelus tukula is 10%; 2), parent reproduction regulating, injecting an ovulation-promoting hormone analogue into parent epinephelus fuscoguttatus, and injecting chorionic gonadotropin and an ovulation-promoting hormone analogue into epinephelus tukula; 3), collecting sperms of the epinephelus tukula, diluting the sperms by using sperm cryopreservation liquid, and performing cryopreservation; 4), performing hybrid insemination, collecting ova, unfreezing sperms, and performing mixed fertilization; 5), hatching to a tail bud stage; and 6), carrying out fry cultivation to obtain fish fries. According to the method, distant hybridization breeding of epinephelus tukula and epinephelus fuscoguttatus is realized, reproductive isolation in the aspects of geographical distribution, propagation time, propagation temperature and the like in the natural environment is overcome, anda new hybrid germplasm with the advantages of fast growth, low oxygen resistance and low temperature resistance is cultivated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
A kind of artificial hybrid breeding method of fast-growing grouper
ActiveCN110521641BDelicious meatHigh nutritional valueClimate change adaptationPisciculture and aquariaAnimal scienceEpinephelus akaara
The invention relates to an artificial hybridization breeding method for groupers high in growth speed, and belongs to the technical field of aquaculture. The method comprises the steps of parent selection, cage culture of parent red-spotted groupers, industrial culture of parent epinephelus tukula, sexual transformation of the epinephelus tukula, freeze preservation of seminal fluid of the epinephelus tukula, spawning induction of the parent red-spotted groupers, artificial insemination and hatching of fertilized eggs. By using the sperm freeze preservation technology, an epinephelus tukula sperm bank is built, reproductive isolation between the two kinds of groupers is broken through, and a new excellent hybrid variety which is high in growth speed is obtained. The problem is solved thatmale parent epinephelus tukula produces fewer sperms, and consequently, production is hindered, the productivity is greatly improved, and grouper breeding work is facilitated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Reproductive isolation control method for cycadopsida
InactiveCN100525609CImprove seed setting ratePromiscuity controlPlant genotype modificationAngiosperms/flowering plantsPromiscuous behaviourCycas
The invention relates to the field of protection of plant germplasm resources, and provides a method for controlling reproductive isolation of cycad plants. The method comprises the following steps: determining the pollen collection time; collecting pollen; determining the pollination time; artificial pollination. Artificial pollination by using the phenomenon of "late ripening" and "specific pollination" of cycads can effectively control the promiscuity among cycads, solve the problem of species mixing in centralized conservation, and improve the seed setting rate of cycads.
Owner:SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
LRR-RLK receptor kinase AtMDIS1 and application thereof in breaking reproductive isolation between species
ActiveCN105505960AImprove fertilization efficiencyImproved efficiency of directionally targeting unfertilized Arabidopsis ovulesTransferasesFermentationArabidopsisKinase
The invention discloses LRR-RLK receptor kinase AtMDIS1 having high expression in arabidopsis plant pollen and a method for breaking a close hybridization barrier through AtMDIS1. AtMDIS1 is expressed in species except arabidopsis through a transgenic means, gene exchange between different species can be promoted, and the hybrid sterility barrier in hybridization breeding is broken. Experiments prove that the method can successively improve arabidopsis ovule recognition efficiency of close species plant male gametophytes.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
A method for obtaining male pronuclei by using oocytes between different species of animals as a medium
ActiveCN110684720BIncrease formation rateIncrease profitNew breed animal cellsEmbryonic cellsAnimal scienceMale pronucleus
The present invention relates to the technical field of embryo engineering, in particular to a method for obtaining a male pronucleus by using oocytes among heterogeneous animals as a medium, comprising the following steps: Step 1, obtaining the mature oocyte of animal A with the first polar body The cells are placed in the in vitro fertilization droplet of animal B; step 2, the sperm of animal B is injected into the in vitro fertilization droplet containing the oocyte in step 1 to obtain a mixed sperm-egg droplet; Step 3. Place the mixed sperm-egg droplet obtained in step 2 in CO 2 Incubation in an incubator until the sperm of animal B enters the cytoplasm of the oocyte to form a male pronucleus; wherein, animal A and animal B are heterogeneous animals. In the method of the present invention, the fertilization biological principle of reproductive isolation of heterogeneous animals without cross-fertilization is utilized, and the method of in vitro fertilization is used to obtain the male pronucleus of another animal with the oocyte of the heterogeneous animal as a medium, and the formation rate of the male pronucleus is as high as 17.8% or more.
Owner:武汉纽利特生物技术有限公司
Artificial interspecific hybridization method for ruditapes variegata and ruditapes philippinarum
ActiveCN103749365BImproved Breeding TraitsBreak through the reproductive isolation barrierClimate change adaptationPisciculture and aquariaInterspecific hybridizationHeterosis
Owner:DALIAN OCEAN UNIV
Method for culturing cenospecies of apis cerana fabricius and apis mellifera linneae
The invention relates to a method for breeding a novel bee species by breeding a hybrid of east bees and west bees, comprising introducing a west bee brood comb going to come out cells into a swarm of east bees preparing for laying eggs, to make the number of east worker bees equal to that of west worker bees, and obtaning a mixed swarm; the east queen bee laying eggs on empty combs, moving out the east queen bee to make the swarm without a queen, after the first generation east worker bees have got out cells; putting in a queen cell frame 20-22 hours later, breeding a queen with four-day old east bee grubs of same swarm to get a novel east queen bee; and hybridizing said queen as mother with west male bee as father by artificial insemination or natural copulation to obtain a hybrid thereof. The method breaks the reproductive isolation between two different species, and the hybrid obtained thereby has a remarkble heterosis.
Owner:云南农业大学东方蜜蜂研究所
Method of obtaining oyster distant intergeneric hybrid
The invention discloses a method of obtaining an oyster distant intergeneric hybrid. The method includes a step of collecting different intergeneric parents, labeling each identified species and synchronously ripening; a step of, after the gonads of the parents are ripe, dissecting sperms and eggs, isolating strictly and performing intergeneric hybridization and matching to obtain a selfing group and a hybridization filial generation group of two parents, and a step of cultivating the filial generation to obtain the survival distant intergeneric hybrid. At the same time, the type of the hybrid is determined by performing heredity identification by utilization of molecule and adoption of cytology means. According to the method, oyster intergeneric hybridization is performed for the first time and the survival intergeneric hybrid is obtained, thus providing a rare scarce material for analysis of oyster genetic differentiation, reproductive isolation and species formation and developing a novel route for genetic breeding of the oyster. The method has advantages of simple operation, high feasibility, and the like.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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