45results about How to "Obvious heterosis" patented technology

The invention relates to the technical field of animal breeding and particularly provides a breeding method of a high-quality synthetic white pig line. The method utilizes existing domestic and overseas pig breed resources, a specialized line is bred according to a trait differentiation and selection principle, thus commercial pigs quicker to grow, higher in lean meat percentage, good in meat quality, strong in resistance and high in slaughtering weight uniformity are obtained through hybrid breeding of the specialized line, the problem existing in 'inner binary' and 'inner ternary' breeding systems can be solved, meanwhile local pig breed resources can be also effectively protected and utilized, high-quality pig breeds are provided for industrial large-scale breeding, and the heavy demands for high-quality pork of masses of consumers are met.

The invention discloses a method for highly efficient assisted selection of molecular markers for japonica rice restorer lines, comprising the following steps: (1) using primers to mark the first and tenth chromosome restorer genes SSR of four rice parents respectively, and then performing amplification and electrophoresis , to obtain the restorer line genotypes of the four rice parents; (2) cross the four rice parents, and then obtain F 2 The molecular marker-assisted selection results of gene population restorer line, in F 2 The restorer line lanes of molecular marker-assisted selection in generation segregation population; (3) for F 4 generation by molecular marker-assisted selection. A large number of restorer lines and a wide range of restorer types can be selected in a short period of time by molecular marker-assisted selection, which saves manpower, material resources and financial resources.

The invention provides a northern high-temperature-resistant pacific oyster new strain culture method. Firstly, pacific oyster and crassostrea nippona parents are collected, synchronous maturity promoting culture of the parents is performed, male and female oysters are distinguished by dissection and microscopy after sexual glands of the pacific oyster and crassostrea nippona parents are synchronously mature, mature eggs of pacific oysters and semen of crassostrea nippona are acquired, cross fertilization is performed to obtain hybrid generations of the two parents, offspring is cultured after cross groups are obtained, the first hybrid generations are determined as pacific oyster and crassostrea nippona first hybrid generations by a microsatellite molecular marker technique method, the hybrid generations are self-reproduced by a butt selection method, and a plurality of generations are continuously bred to obtain a high-temperature-resistant pacific oyster new strain. The obtained pacific oyster new strain has the advantages of high temperature resistance, high survival rate, growing rapidness, high dressing percentage and the like.

The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.