45results about How to "Obvious heterosis" patented technology

The invention relates to a method for breeding a novel bee species by breeding a hybrid of east bees and west bees, comprising introducing a west bee brood comb going to come out cells into a swarm of east bees preparing for laying eggs, to make the number of east worker bees equal to that of west worker bees, and obtaning a mixed swarm; the east queen bee laying eggs on empty combs, moving out the east queen bee to make the swarm without a queen, after the first generation east worker bees have got out cells; putting in a queen cell frame 20-22 hours later, breeding a queen with four-day old east bee grubs of same swarm to get a novel east queen bee; and hybridizing said queen as mother with west male bee as father by artificial insemination or natural copulation to obtain a hybrid thereof. The method breaks the reproductive isolation between two different species, and the hybrid obtained thereby has a remarkble heterosis.

The invention discloses a method for breeding a meat and wool dual-purpose new variety of Gansu alpine merino, which comprises: crossing a South African mutton merino serving as a male parent and a Gansu alpine merino serving as a female parent, and selecting first-generation hybrid female merinos; crossing the select first-generation hybrid female merinos with South African mutton merinos, and selecting second-generation hybrid male and female merinos; crossing the selected second-generation hybrid female merinos with South African mutton merinos and selecting third-generation hybrid female merinos; mating the third-generation hybrid female merinos with the selected second-generation hybrid male merinos to breed the zero-generation merinos of the meat and wool dual-purpose new variety of Gansu alpine merino; selecting high-quality male merinos and female merinos; and performing 3 to 4 generations of crossing by a group secondary selection method and obtaining the meat and wool dual-purpose new variety of Gansu alpine merino. The merinos bred by the breeding method have the advantages of tall and big body, high lambing percentage, quick growth, high wool quality, high meat quality and the like, and are suitable for drylot feeding and pen feeding for fattening. The method is in accordance with the development trend of sheep breeding industry of China.

The invention belongs to the technical field of poultry genetic breeding, and particularly relates to a hybrid seed production method of high uniformity yellow chicken three-line combination suitablefor chilled marketing. According to the method, a slow-feathering, yellow-feathering and green-shank chicken line is adopted as a first male parent, a fast-feathering, yellow-feathering, yellow-shank,high-yield and high-quality chicken line is adopted as a first female parent, and hybridization is conducted to obtain an F1 female parent; a local chicken line which is fast-feathering, and yellow-feathering, jute-feathering, sexual precocity and big cockscomb in appearance with the weight within +/-10% of the average weight is selected as a terminal sire, and hybridization is conducted betweenthe terminal sire and the F1 female parent to obtain commercial generation with high uniformity. According to the method, by means of regular and molecular breeding, selections of physical conformation, sexual precocity, weight uniformity and slaughter traits and comprehensive selection of meat quality traits are focused on and carried out, the bred yellow chicken commercial generation is high inuniformity and survival rate, and significant in heterosis; and the chicken has big cockscomb, green shank, fine pores and firm skin.

The invention discloses a breeding method of a novel variety of red-rooted salvia. The breeding method of the novel variety of red-rooted salvia includes using a male sterile line SH-B-18 of red-rooted salvia as a female parent and a fertile line A-107 of red-rooted salvia as a male parent, and preparing the female parent and the male parent into a hybrid of red-rooted salvia. The breeding method of the novel variety of red-rooted salvia is applicable to novel high-yield, stable-yield, high-quality, disease-resistant and highly-applicable hybrid varieties of red-rooted salvia with fine comprehensive agronomic characters, which are planted in a base of Shangluo red-rooted salvia, even in same planting areas of China, and is applicable to the technical field of breeding.

The invention relates to the technical field of agriculture bioengineering and particularly relates to a cultivation method of new strawberry species. The method includes steps of: pushing petals aside of female parent flowers in a big bud stage; pulling up all the stamens; picking male parent flowers to pollinate the female parent flowers; covering the flower heads with paper bags after pollination; removing the paper bags when receptacles begin expand after 10-15 d and bear young fruits; picking mature hybrid fruits after 30-40 days successively; cleaning seeds, air-drying; putting the seeds into a refrigerator; refrigerating at 1-3 DEG C and keeping for further use; sieving sandy soil with a sieve having a size of 1-2.5 mm, autoclaving, disinfecting and seeding; and after the seeds survive, planting seedlings having 4-5 leaves to a greenhouse, accelerating culture, and performing strain evaluation screening in fields to obtain the screened new strawberry species. By adoption of a crossbreeding method, strawberry individuals with different excellent properties are hybridized, and through genetic recombination, individuals with heterosis are obtained.

The invention discloses a molecular marker related to chicken carcass characters and an acquisition method and application thereof. The molecular marker related to the chicken carcass characters is single base mutation of a ninth exon G+1479A positioned at a fat mass and obesity associated (FTO) gene. The molecular marker is obtained by cloning full length of a chicken FTO gene by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) technology, wherein a full-length sequence and a coding region sequence of the FTO gene are shown as SEQIDNO:1-2. Correlation analysis of the chicken carcass characters shows that the carcass weight, wing weight, leg muscle weight, half eviscerate weight and full eviscerate weight of a 90-day-old mutated individual are obviously higher than those of a wild type individual.

The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.

The invention provides a new ostrea rivularis species breeding method. Firstly, ostrea rivularis and crassostrea gigas parents are selected, and oyster parent ripening promotion breeding is conducted; secondly, ostrea rivularis and crassostrea gigas are manually dissected, female ostrea rivularis and male crassostrea gigas are selected in a microscopic examination mode, and eggs of ostrea rivularis and sperms of crassostrea gigas are taken out for fertilization to form hybrid fertilized ovums of ostrea rivularis; thirdly, after incubation of the hybrid fertilized ovums, larvae are selected and bred in a larva breeding pond; finally, settlement and metamorphosis are conducted on the bred larvae, and a first generation of hybrid fry of ostrea rivularis for breeding is bred, and through artificial breeding, new ostrea rivularis species which is high in disease resistance and growing speed and good in meat quality is screened out to be bred.

The present invention discloses a method of selectively breeding, reproducing and preparing hybrid seed of Caitai cytoplasm male sterile line. The present invention adopts sterile source Chinese cabbage CMS3411-7 as female parent and Liuzhou Caitai selfed line as male parent, to breed the Caitai cytoplasm male sterile lines TC1-3-1 and TC2-8 through backcross for several generations, pair matching and strict selection. The Caitai cytoplasm male sterile lines TC1-3-1 and TC2-8 have stable sterility, normal pistil function, healthy honey cup, no yellowing in seedling stage and excellent natural pollination. The bred hybrid varieties TC1-3-1x TS14 and TC2-8 x TS46 have fast growth speed, high yield, high quality and high disease resistance.

The invention discloses a method for breeding novel variety of Brassica oleracea by using two DH (dihaploid) lines, comprising: hybridizing a cytoplasmic male-sterile line DHCMS08-5-3-6-9 trans-bred with a Brassica oleracea DH line as a female parent and the Brassica oleracea DH line DH05X-1-5-9-8 line as a male parent to prepare DHCMS08-5-5-6-9*DH05X-1-5-9-8 hybrid first-generation combination, named as Qingan 1562. The method of the invention is a method to breed a mid-early maturity novel variety of Brassica oleracea by using two DH lines, a novel hybrid first-generation variety of Brassica oleracea is bred through DH liens with genotype purity of 100% created by culturing isolated microspores of Brassica oleracea, the novel hybrid first-generation variety of Brassica oleracea has highly uniform economic traits and completely exhibited target traits, and the breeding of disease-resistant high-quality high-yield variety breeding targets is achieved.

The invention discloses an outstanding fairy mushroom crossbreeding method which comprises the following steps that (1) a white jade mushroom bacterial strain and a gray crab mushroom bacterial strainare cultivated respectively to obtain corresponding right-age hyphae; (2) a protoplast is prepared and generated: under the aseptic condition, the right-age hyphae obtained in the step (1) are subjected to enzymolysis treatment respectively, the protoplast is obtained, purification is performed, a protoplast regeneration culture solution is added, and sealing culture is performed; (3) a tip hyphaof the regenerated protoplast is selected for culture, a mononuclear regeneration bacterial strain is screened under the aseptic condition, then the white jade mushroom bacterial strain and the graycrab mushroom bacterial strain are subjected to pairing and crossbreeding, inoculated culture and purification are performed, and the outstanding fairy mushroom is obtained.

The invention discloses a pond cultivation method for current-year fries of half-bred takifugu, and the method is characterized in that the method comprises the steps: carrying out desilting and disinfecting of a pond before the fries are put in the pond; splashing compound fertilizer cultivation biology baits after water enters into the pond, wherein the body length of each fry needs to be greater than 8 mm, and the activity of the fries is high; enabling the water salinity of the pond to be gradually increased to the pond cultivation salinity before the fries are put in the pond, wherein the fries needs to be in water for carrying, and the cultivation density of the fries is 4500/1000 square meters; casting no bait at the initial stage of fry throwing; splashing soybean milk and frozen big artemias when the biology baits in the pond is not sufficient, thereby reducing the mutual killing of the fries, and improving the survival rate of the fries; casting cluster eel cooperation baits after the body length of the fries reaches 20mm; changing water one time per 15 days at the early stage of cultivation; adjusting the water-changing frequency to be one time per 7 days at the middle and later stages of cultivation; splashing a biological agent into the pond in a cultivation process, so as to prevent parasites; transferring half-bred fishes to a thermal-insulation greenhouse for overwintering when the temperature of water in the pond decreases to 18 DEG C or less, wherein the catching mode is a mode of trawling, and a knotless net is used for trawling, thereby avoiding the scratching damage to the fish body.

The invention discloses a method for breeding and producing a new variety of a commercial line of eriocheir sinensis. The method comprises the following steps: (1) parent selection of the commercial line: selecting commercial selection lines A and B of eriocheir sinensis for serving as parents; (2) production of the commercial line: performing diallel crossing on the commercial selection lines A and B to produce the commercial line; (3) screening of the commercial line: screening an A-female and B-male commercial line which takes line A as a female parent and takes line B as a male parent through growth contrast test; (4) performing productive verification on the commercial line; (5) applying the new variety of the commercial line. By adopting the new variety of the commercial line bred and produced through the method, the major advantages of the female parent A and the male parent B are well combined, high growth speed, high group yield and remarkable hybrid vigor are realized, and excellent provenance is provided for the river crab industry in China. The new variety is the first variety of the commercial line of eriocheir sinensis in China.

The invention disclose a new product of edible mushrooms. The new product of the edible mushrooms is obtained from hybridization breeding by adopting the following steps (1), a white beech mushroom bacterial strain and a gray crab mushroom bacterial strain are cultivated respectively and corresponding hypha of the right age are obtained; (2), a protoplast is prepared and regenerated, specifically,under an aseptic condition, the hypha of the right age obtained in the step (1) are subjected to enzymolysis treatment respectively, the protoplast is obtained, purifying is conducted, a protoplast regeneration culture solution is added, and sealing culture is conducted; and (3), top end hypha of the regenerative protoplast is selected and taken to be subjected to culture, mononuclear regenerative bacterial strains are screened under an aseptic condition, then a white beech mushroom mononuclear bacterial strain and a grey cab mushroom mononuclear bacterial strain are subjected to matching hybridization, and the new product can be obtained after inoculated culture and purification are conducted.

The invention discloses a method of utilizing cabbage CMS and DH lines to selectively breed early-ripening new variety. The method includes following steps: selecting cabbage cytoplasm male sterile line DH08-GW100M3CMS as a female parent and DH line DH12-3-M2 as a male parent for hybridization to breed a DH08-GW100M3CMSxDH12-3-M2 combination which is named as a qin'gan 658 variety. Variety comparison experiments, variety area experiments and production demonstration of the variety in Yang Ling, Xi'an and Baoji Taibai which are major vegetable cultivation areas in Shanxi province show that the variety is remarkable in hybrid vigor, excellent in comprehensive economic character, resistant to head bursting, high in quality, disease-resistant, outstanding in yielding ability, high in adaptability and stable in yield increase advantage, and yield is increased by 10.2-14.3% when compared with that of a cabbage variety 8398.