The invention discloses a vagina cleaning care lotion and a preparation method thereof. The vagina cleaning care lotion for external use is prepared from raw materials as follows: radix sophorae flavescentis, phellodendron bark, fructus cnidii, borneol, tea tree oil, rosemary, alum, hydrolyzed collagen, polyhexamethylene biguanidine hydrochloride, chlorhexidine acetate and water; after radix sophorae flavescentis, fructus cnidii and phellodendron bark are boiled with water in proportion and extracted, an extracting solution is concentrated to the relative density of 1.01-1.04, chlorhexidine acetate, borneol, tea tree oil, rosemary, alum, hydrolyzed collagen, polyhexamethylene biguanidine hydrochloride and water are added, and the anti-germ care lotion suitable for vagina cleaning of both men and women is obtained through mixing, filling and packaging. The lotion has the efficacy of clearing heat, removing toxicity, clearing dampness and killing parasites to relieve itching, inhibits growth of harmful germs of the vagina, clears away peculiar smell and effectively relieves female vulvitis, vaginal itching and other symptoms. The formula is reasonable, and the vagina cleaning care lotion doesn't have toxic and side effects or allergic reaction and has good treatment and prevention functions on gynecological diseases such as germ infection of the vulva, the vagina and the urethra and the like.