33 results about "Sexual precocity" patented technology

The invention belongs to the technical field of poultry genetic breeding, and particularly relates to a hybrid seed production method of high uniformity yellow chicken three-line combination suitablefor chilled marketing. According to the method, a slow-feathering, yellow-feathering and green-shank chicken line is adopted as a first male parent, a fast-feathering, yellow-feathering, yellow-shank,high-yield and high-quality chicken line is adopted as a first female parent, and hybridization is conducted to obtain an F1 female parent; a local chicken line which is fast-feathering, and yellow-feathering, jute-feathering, sexual precocity and big cockscomb in appearance with the weight within + / -10% of the average weight is selected as a terminal sire, and hybridization is conducted betweenthe terminal sire and the F1 female parent to obtain commercial generation with high uniformity. According to the method, by means of regular and molecular breeding, selections of physical conformation, sexual precocity, weight uniformity and slaughter traits and comprehensive selection of meat quality traits are focused on and carried out, the bred yellow chicken commercial generation is high inuniformity and survival rate, and significant in heterosis; and the chicken has big cockscomb, green shank, fine pores and firm skin.

The invention relates to a compound pig feed which is prepared by the following components by weight: 0-5 parts of alfalfa, 0-5 parts of green grass, 25-32 parts of green wheat entire stool powder, 15-20 parts of bean pulp, 8-13 parts of bran, 0.5-1 part of bone meal, 1-3 parts of fish meal, 0.3-0.5 part of sodium chloride, 12-18 parts of carrot, 2-4 parts of cysteine, 0.5-1 part of mountain flour and 2-5 parts of spirulina powder. The compound pig feed is not added with any hormone, so that the side effect that the hormones cause people to come fat and cause children to have sexual precocity is avoided; and the produced pork is good in meat quality, fresh, tender, succulent, high in lean meat percentage and good in taste and has strong market competitiveness.

The invention relates to a method for culturing river crabs by utilizing the early breeding crab larvae, in particular to a method, by which the river crabs are cultured in a pond or other water areas and become large-size commercial crabs. The method is characterized in that the method comprises the steps of (1) purchasing the early breeding crab larvae: the selection of the crab larvae purchasing date, the selection of the crab larvae quality, and the transportation of the crab larvae; (2) culturing the early culturing crab larvae: the disinfection of the culture pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the cultivation of water grass, the stocking of the early breeding crab larva, and the feeding of artificial food; (3) controlling the gonad development speed of crabs: the crabs getting through the summer artificially, the crabs getting through the winter and grow naturally, the stocking of the crabs, the management of the crab larvae getting through the winter, the promotion of the river crab for exuviation and growth, and the culture and the harvesting of the river crab. The crabs, the gonad of which is immature are cultured by utilizing the early breeding crab larvae produced at the beginning of December to the end of the April in the ensuing year; the growing period of the early breeding crab larvae can be prolonged, and the sexual precocity can not happen; through the artificial over-summer and the natural over-winter technologies, the early breeding crab larvae can become the crabs for the river crab culture; the crabs cultured by utilizing the early breeding crab larva can be cultured into the grown crab with the specification being 200 to 400g for each.

The invention relates to a method for cultivating medium-speed high-quality spotted-brown chickens suitable for slaughtering and living body marketing. The method comprises the following steps: takingslow-feathering, yellow-shank, yellow-skin and recessive white-feathering chickens (B line) as a first female parent; and taking fast-feathering, yellow-shank, yellow-skin, spotted-brown-feathering and big-cockscomb chickens (C line) as a first male parent. According to the genetic principle of fast and slow feathering self-sexing, a parent generation (BC line) is produced by crossing a B line and a C line to serve as a terminal female parent, parent generation chicks can self-sexing through fast and slow feathering, hens are fast-feathering chickens, and cocks are slow-feathering chickens. The commercial generation (ABC) is produced by using fast-feathering, yellow-shank, yellow-skin, large-cockscomb and spotted-brown-feather fast large line (A line) as a terminal male parent and three line mating hybridization of male A line X (male B line X female C line) female, commercial generation cocks have the characteristics of fast feathering, yellow shank, yellow skin, sexual precocity, large cockscomb, wide chest and the like. The commercial generation is marketed when at the age of 90-95 days, the carcass is beautiful, the feather color is bright, the cockscomb is large, the meat production performance is good, the meat quality is good, the carcass packaging property of high-quality chickens and the packaging property of living body marketing are obviously identified, and the marketing standard of medium-speed slaughtered high-quality chickens and live chickens is met.

The invention discloses an Eriocheir sinensis sexual precocity property related SNP marker and its application. The SNP is positioned in a 3'-UTR region of ecdysis inhibition hormone gene MIH(AY310313.1) and is concretely positioned in 3088bp, and base variation information is SNPA3088T T>G. Property-genotype relevance analysis results show that a significant correlation exists between SNP mutation and river crab sexual precocity properties, wherein the sexual precocity property risk of the GG genotype river crab individuals is 2.175 times higher that of TT genotype individuals. The polymorphism SNP marker of the river crab sexual precocity related gene, and can be used for the subsequent and river crab sexual precocity property related genetic and breeding researches.

The invention discloses a freshwater shrimp opsin gene, a protein encoded by the same and application. The nucleotide sequence of the gene is SEQ ID NO: 1, and the nucleotide sequence encoded by the gene is SEQ ID NO: 2. A molecular mechanism for regulation and control of key genes related to exogenous environmental factors to development of ovary of crustacean, and a theoretical basis is providedfor sexual precocity of freshwater shrimps; and (2), dsRNA which is synthesized according to the nucleotide sequence of the freshwater shrimp opsin gene is injected into the cardiocoelom of a freshwater shrimp, vitellogeningene expression in the ovary of the freshwater shrimp can be relieved effectively, and therefore, the freshwater shrimp opsin gene has quite important influences to developmentof the ovary of the freshwater shrimp.

The invention discloses a macrobrachium nipponense FoxL2 (Forkhead box protein L2) protein as well as a coding gene and an application thereof. An FoxL2 gene is cloned from testes or ovaries of the macrobrachium nipponense by using primer groups provided by the invention, dsRNA is prepared by using the FoxL2 gene, after the dsRNA is injected into the pericardial cavity of male or female macrobrachium nipponense, the expression of the FoxL2 gene in the testes or ovaries can be silenced, the development of the testes of the macrobrachium nipponense can be delayed, but the influence on the development of the ovaries of the macrobrachium nipponense is not obvious. Thus, the FoxL2 gene of the macrobrachium nipponense can be used for promoting the development of the testes and has the function against the action of the FoxL2 gene in mammals and fishes. According to the macrobrachium nipponense FoxL2 protein as well as the coding gene and the application thereof, new thinking and effective means are provided for overcoming the sexual precocity phenomenon of the macrobrachium nipponense and breeding good varieties.

The invention discloses a traditional Chinese medicine composition for treating liver depression and phlegm stagnation type sexual precocity of children and a preparation method and quality detection method thereof, belonging to the field of traditional Chinese medicine. The traditional Chinese medicine composition oral solution for treating sexual precocity of children is prepared based on the traditional Chinese medicine theory from the following traditional Chinese medicinal materials: radix scutellariae, root-bark of peony, cape jasmine, radix bupleuri, tuckahoe, trichosanthes bark, angelica sinensis, radix curcumae, dried orange peel, thunberg fritillary bulb, raw oyster, phellodendron bark, nutgrass galingale rhizome, burreed tuber, white atractylodes rhizome, white peony root and the like. The traditional Chinese medicine composition has the efficacies of soothing the liver, regulating the flow of qi, reducing fever, promoting blood circulation, softening hard masses and resolving phlegm, and is suitable for treating the liver depression and phlegm stagnation type sexual precocity of children. The traditional Chinese medicine composition can effectively suppress premature and fast development of the sexual gland and quick epiphyseal arrest, slows down the growth speed of skeletal age, maintains normal growth speed of children and improves the eventual body height of children. The traditional Chinese medicine composition has no obvious toxic or side effect after being taken by children, and has low price, good social effect and economic benefit and wide market development prospect, and the responses of children and parents are generally good.

The invention relates to the field of medicinal chemistry and in particular relates to 4-imidazolyl quinoline and quinazoline ketone aromatizing enzyme inhibitors. The invention further discloses a preparation method and pharmacological activity of the inhibitors, a medicinal composition containing the compounds as well as the medical application of the inhibitors, in particular the medical application of the inhibitors in preventing or treating estrogen maintaining diseases such as breast cancers, endometrial cancers, female sexual precocity, gynecomastia and the like.

The invention discloses a molecular marking method for two mutation loci in a 5' regulatory region of a chicken MMP-11 gene and an application in breeding of chicken sexual precocity traits. Three mutation loci, namely g.-889 (C>T), g.-636 (A>G) and g.-518 (C>T) loci, are found out to exist in the 5' regulatory region of the chicken MMP-11 gene for the first time, wherein two SNP loci of g.-889 (C>T) and g.-636 (A>G) have significant correlation with the chicken laying starting day age and the egg laying number in the earlier stage of 30 weeks of age; the CA / CA genotype corresponds to the smaller laying starting day age and the higher egg laying number in the earlier stage, and the individual of the TG / TG genotype starts to lay late correspondingly, so that detection of the two molecular marker loci associated with sexual maturity traits is helpful for breeding sexual maturity variety / lines, beneficial help is provided for chicken breeding work, especially for local chicken breeds withlate sexual maturity, and the accurate and effective marker loci are also provided for customization of a molecular marker-assisted breeding chip.

The invention relates to a method for culturing river crabs by utilizing the early breeding crab larvae, in particular to a method, by which the river crabs are cultured in a pond or other water areas and become large-size commercial crabs. The method is characterized in that the method comprises the steps of (1) purchasing the early breeding crab larvae: the selection of the crab larvae purchasing date, the selection of the crab larvae quality, and the transportation of the crab larvae; (2) culturing the early culturing crab larvae: the disinfection of the culture pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the cultivation of water grass, the stocking of the early breeding crab larva, and the feeding of artificial food; (3) controlling the gonad development speed of crabs: the crabs getting through the summer artificially, the crabs getting through the winter and grow naturally, the stocking of the crabs, the management of the crab larvae getting through the winter, the promotion of the river crab for exuviation and growth, and the culture and the harvesting of the river crab. The crabs, the gonad of which is immature are cultured by utilizing the early breeding crab larvae produced at the beginning of December to the end of the April in the ensuing year; the growing period of the early breeding crab larvae canbe prolonged, and the sexual precocity can not happen; through the artificial over-summer and the natural over-winter technologies, the early breeding crab larvae can become the crabs for the river crab culture; the crabs cultured by utilizing the early breeding crab larva can be cultured into the grown crab with the specification being 200 to 400g for each.

The invention provides a method for cultivating arctic charr triploid offspring seeds. Oosperms of arctic charrs are placed at 6-8 DEG C, the oosperms are fertilized for 12-14 min, then the oosperms are moved into a 6-DMAP (6-Dimethylaminopurine) solution with concentration being 220-240mg / L and temperature being 9-11 DEG C to be socked for 14-16 min, and the oosperms are moved into a 6-8 DEG C water body to be normally incubated. After about 60 days, fries of the arctic charrs are incubated from the oosperms. During the incubating process, developmental rate of the oosperms is above 61 %, the hatching rate of the fries reaches above 90%, the obtained fries are detected through a flow cytometry, and the triploid rate reaches 79%. According to the method for cultivating the arctic charr triploid offspring seeds, the problems, caused by long-time near relation propagation and shortage of systematic seed selection, of sexual precocity, individual miniaturization, disease breeding and the like in a breeding individual are relieved, and the method has good market value.

The invention discloses a culture method of river crab megalops larvae and relates to the technical field of crab breeding. The culture method comprises the following steps: constructing a nursery pond, planting aquatic plants, breeding parent fries outside cages, feeding baits, carrying out water quality management and carrying out greenhouse management. By adopting the method disclosed by the invention, the survival rate of the river crab megalops larvae can be improved; the megalops larvae have the advantages of rapid development, large size, high yield, low sexual precocity rate and easiness for management; and after the megalops larvae are cultured into phase-V juvenile crabs, the phase-V juvenile crabs can be transferred into a juvenile crab culture phase by ponds to be cultured, andthe culture method has very good culture popularization value.

The invention discloses a traditional Chinese medicine pill for treating female sexual precocity syndrome and a preparation method thereof, and belongs to the field of traditional Chinese medicine. The effective component of the pill is composed of the following raw materials by weight: 68 to 82 grams of jujube, 65 to 78 grams of wolfberry, 63 to 76 grams of American ginseng, 60 to 75 grams of white hyacinth bean, 58 to 70 grams of black sesame, 56 to 67 grams of polygonatum rhizome, 53 to 63 grams of rehmanniae radix, 48 to 60 grams of poria cocos, 44 to 56 grams of millet sprout, 41 to 55 grams of fructus citri, 38 to 52 grams of bighead atractylodes rhizome, 35 to 50 grams of sophora flower, 33 to 48 grams of mother of pearl, 30 to 45 grams of fleece-flower root, 28 to 42 grams of chrysanthemum, 26 to 39 grams of radix bupleuri, 24 to 36 grams of licorice, 21 to 32 grams of cyperus rotundus, 18 to 28 grams of white peony root, 12 to 25 grams of raw gardenia jasminoides, 9 to 20 grams of anemarrhena, 6 to 15 grams of ligustrum lucidum, 4 to 10 grams of sweet wormwood, and 1 to 6 grams of toosendan fruit. The selected traditional Chinese herbals are in accord with the principle of monarch, minister, assistant and guide in traditional Chinese medicine. The formula is developed to treat female sexual precocity syndrome. The pill has the functions of invigorating spleen, harmonizing the stomach, nourishing yin, tonifying kidney, clearing heat, and soothing the liver, has the advantages of good absorbing effect, prominent effect, no side or toxic effect, and no clinical adverse reaction, and has a high cure rate in clinic.

The invention provides a cultivation method of large-specification juvenile eriocheir sinensis. The cultivation method comprises the steps of selecting a crab pool, regulating the water quality, planting alternanthera philoxeroides, selecting juvenile crabs, feeding the juvenile crabs, harvesting and the like. The breeding variety is 'Noah No. 1' of eriocheir sinensis, is a new variety of river crabs certificated by the National Aquatic Stock Seed and Fine Breed Certification Committee in 2016 and has the advantages of being high in growth speed, high in large-specification rate and the like.The planted aquatic plants are mainly alternanthera philoxeroides, alternanthera philoxeroides has a strong purifying capability, during high temperature in summer, the aquatic plants are not rotted,the water quality is not deteriorated, and it is guaranteed that the pool has good water quality in the breeding cycle. The specification of the put juvenile crabs is 140,000-160,000 per kg, 1.0-1.25kg of the juvenile crabs are put per mu, 200 kg or above of the juvenile crabs are harvested, the proportion of the large-specification juvenile crabs reaches 70% or above, and the problems that the juvenile crabs are subjected to sexual precocity, small in size, poor in quality and low in economic benefit are solved.

The invention relates to serum miRNA markers for measuring a chicken sexual maturity starting time and a preparation method of marker-containing serum. The marker is any one or any combinations of gga-miR-29c-3p, ggamiR-217-5p, gga-miR-375, gga-miR-215-5p, gga-miR-19b-3p, gga-miR-133a-3p and gga-let-7a-5p. By extracting chicken serum small RNA and by detecting the expression law of the miRNA markers in a sexual maturity progress by virtue of a qRT-PCR technology, seven specific cyclic miRNA markers, which are capable of accurately measuring the chicken sexual maturity starting time, are identified; therefore, a more accurate measuring standard for conducting chicken sexual maturity starting mechanism researches and the breeding of a novel chicken line (variety) of sexual precocity is provided.

The invention relates to the technical field of production of tablets, particularly relates to a production method of pine pollen tablets for treating sexual precocity of children. The production method comprises the following steps: removing impurities from pine pollen, performing wall-breaking treatment by airflow grinding, soaking in water of normal pressure and temperature, performing ultrasonic treatment, centrifuging with a refrigerated centrifuge, collecting supernatant, and separating pine pollen water-soluble extract of molecular weight less than 4,000 D at 1-5 DEG C, and lyophilizing the pine pollen water-soluble extract to obtain pine pollen lyophilized powder; and mixing pine pollen lyophilized powder with lecithin, water-soluble starch and microcrystalline cellulose PH102, granulating, tabletting, and coating. The pine pollen tablets provided by the invention retain the activity of functional components, and have the advantages of no adverse effects, no liability of oxidative deterioration, and easy absorption.

The invention provides a preparation method of an oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed. The preparation method comprises the steps of: carrying out in vitro fertilization on oncorhynchus mykiss walbaum female parent fish ova and oncorhynchus mykiss male parent fish sperms, transferring into a 170-190 mg / L 6-DMAP (6-dimethylaminopyridine) solution with the temperature of 12-14 DEG C after fertilized ova is fertilized for 12-14minutes, soaking for 14-16minutes, then transferring in a water body with the temperature of 6-8 DEG C and then hatching; hatching the fertilized ovum to obtain hybrid triploid larva fishes after about 50 days; and detecting the larva fishes by using a flow cytometry, wherein the eyed rate of the fertilized ova in a hatching period is above 71 percent, the hatching rate of the seeds reaches above 90 percent and the triploid rate reaches above 80 percent. By using the preparation method of the oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed, the problems of sexual precocity, miniaturization, disease propagation and the like of individuals in a trout cultivating process are solved, the advantages of hybridization and triploid are obtained, and a good market value is achieved.

The invention discloses a method for producing pine pollen nutrition powder for treating child sexual precocity, which relates to the technical field of the production of nutrition powder of products. The method comprises the following steps of: crushing impurity-removed pine pollen through airflow for wall-breaking treatment, then soaking in normal-pressure normal-temperature water, treating by using ultrasonic wave, centrifugally treating in a refrigerated centrifuge with the rotating speed of between 8,000 and 10,000 rpm, collecting supernatant, and then sieving the supernatant by using a molecular sieve with the aperture less than 4,000D at the temperature of between 1 and 5 DEG C to obtain pine pollen aqueous solution with the molecular weight less than 4,000D; freezing and vacuum-drying the pine pollen aqueous solution with the molecular weight less than 4,000D to obtain lyophilized pine pollen with the water content lower than 5 percent; and uniformly mixing the lyophilized pine pollen, milk powder and carrot meal to obtain the pine pollen nutrition powder for treating the child sexual precocity. The pine pollen nutrition powder for treating the child sexual precocity has no side effect, and is low in cost and simple and easy.

The invention discloses a medicine composition for treating sexual precocity and application of the medicine composition. The medicine composition is prepared by the following Chinese crude drugs in part by weight: 11-17 parts of dried rhizome of rehmannia, 18-30 parts of raw oyster, 8-13 parts of hairyvein agrimony, 8-13 parts of marine alga, 9-16 parts of fiveleaf akebia fruit, 9-16 parts of Chinese holly leaves, 5-10 parts of Chinese tamarisk tops, 4-8 parts of polygala roots and 4-8 parts of Flos Magnoliae Officinalis. The medicine composition has effects of nourishing kidney-yin and reducing ministerial fire; when the medicine composition is used for treating sexual precocity, signs and symptoms can be improved effectively; the medicine composition is suitable for treating sexual precocity of girls suffering from low-grade or medium-grade sexual precocity; a clinical therapeutic effect is obvious and course of treatment is short; and the medicine composition takes effect quickly, is safe and does not have toxic or side effects.

According to a male rat fertility comprehensive evaluating system and a judging criteria thereof, 8-9-week-old male rats and female rats with healthy appearance, sensitive response, up-to-standard weight, bright fur color and well-developed sexual organs are selected respectively and bred in independent cages, the rats freely take breeding feeds and drink water and are bred alternately in light for 10 hours and in darkness for 14 hours, the female rats with sexual precocity or vaginal valgus holes in the 4-5 week are abandoned, the estrus female rats are selected according to vaginal smears, the natural estrus female rats and the selected male rats are put into one cage according to the ratio of 1:1 for mating, vaginal plugs of the female rats are detected 10-12 hours after the female ratsand the male rats are put into one cage, the female rats are put into a quiet environment to be carefully nursed, and the number of pregnancy days, litter size and fetal survival number of the femalerats are counted. The fertility of the male rats can be effectively and accurately authenticated, the high-fertility male rats are screened out, and the high-fertility male rats are effectively protected against surgical injuries. The judging criteria has high pertinence and measurability; through a measurable score comparison criteria table, detection is more analyzable and objective, and the fertility of the male rats is visually judged.

A traditional Chinese medicine composition for treating sexual precocity comprises the following raw materials: oriental wormwood, monkshood, semen cuscutae, poria cocos, radix ophiopogonis, tangerine peel, rhizoma pinelliae preparata, fructus aurantii, periostracum cicadae, gorgon fruit, ginkgo seeds, radix rehmanniae preparata, angelica sinensis, radix asparagi, gentian, fructus lycii, cistanche salsa, rhizoma chuanxiong, hawthorns, paeonia suffruticosa, fried gardenia, snakegourd peel, calcined oyster, rhizoma alismatis, glossy privet fruit, cortex lycii radicis, purslane, root of kudzu vine, balsam pears, radix glycyrrhizae, salviae miltiorrhizae, spiranthes sinensis, caltrop, herba ecliptae and polygonum multiflorum. The traditional Chinese medicine composition has high efficiency and no side effect, and is low in cost and suitable for treating various types of child sexual precocity.

The invention discloses a method for culturing high-purity primary Leydig cells of Guizhou fragrance pigs. The method is characterized by comprising the following steps: (1) digesting testicular tissue blocks of fragrance pigs through collagenase to obtain a cell suspension solution; (2) repeatedly digesting with 0.25 percent Trypsin-EDTA to obtain Leydig cells; (3) enriching the Leydig cells through a differential centrifugation method, wherein the differential centrifugation method at least comprises three times of centrifugation, the speed of the first-time centrifugation is 800 to 1200 rpmand the time is 3 to 8 min, and the speed of the second-time and third-time centrifugation is 600 to 1000 rpm and the time is 3 to 8 min; (4) purifying the Leydig cells through a differential attachment method. Compared with an existing primary culture and purification method of pig Leydig cells, the method has the advantages that the operation is simpler and more efficient, and the Leydig cellsof the Guizhou fragrance pigs, which have a great quantity, strong activity and high purity, are obtained. The method disclosed by the invention can provide effective technological guidance for deeplyresearching sexual precocity properties of the Guizhou fragrance pigs and related functions of the Leydig cells.

The invention provides a method for cultivating arctic charr triploid offspring seeds. Oosperms of arctic charrs are placed at 6-8 DEG C, the oosperms are fertilized for 12-14 min, then the oosperms are moved into a 6-DMAP (6-Dimethylaminopurine) solution with concentration being 220-240mg / L and temperature being 9-11 DEG C to be socked for 14-16 min, and the oosperms are moved into a 6-8 DEG C water body to be normally incubated. After about 60 days, fries of the arctic charrs are incubated from the oosperms. During the incubating process, developmental rate of the oosperms is above 61 %, the hatching rate of the fries reaches above 90%, the obtained fries are detected through a flow cytometry, and the triploid rate reaches 79%. According to the method for cultivating the arctic charr triploid offspring seeds, the problems, caused by long-time near relation propagation and shortage of systematic seed selection, of sexual precocity, individual miniaturization, disease breeding and the like in a breeding individual are relieved, and the method has good market value.

The invention relates to the technical field of medical equipment, and especially relates to an apparatus for preventing and treating myopia by exercising scotopic vision and an application method thereof. The apparatus comprises a frame, eyeglasses and glass arms, and shading members used for reducing light transmittance are arranged at the glass arm. The eyeglass having different shading membersprovide special control for the light transmittance, an object contour can be clearly seen while in use for a user, color of the object cannot be clearly seen, and purposes for exercising scotopic vision and preventing myopia can be achieved. When students have a language class and visual sense is not used, the apparatus of the invention is worn for exercising scotopic vision and preventing myopia; under weak light, the usage of the apparatus can offset myopia development, the photochemical damage and non visual effect of eyes can be reduced, the apparatus can directly prevent and treat myopia, and is capable of preventing and treating sexual precocity and obesity by preventing and treating endocrine dyscrasia, and can reach the purposes of health caring and myopia controlling. The apparatus has the advantages of strong operationality and easy popularization.

The invention discloses a growth promoting preparation and an H-OL duplex height increasing system. The growth promoting preparation is prepared from zinc gluconate, fructus alpiniae oxyphyllae, biological calcium carbonate, L- lysine hydrochloride and taurine according to a weight ratio of 1:55:46:11:6. The growth promoting preparation and the H-OL duplex height increasing system are jointly applied to the field of teenager growth promotion. By adoption of a HGH (human growth hormone) hypophysis natural stimulation system, high-concentration nano Chinese medicinal formulas, the growth promoting preparation and an OL chondrocyte activation therapy, cell motility of osteoblasts, chondrocytes, tendon bursal cells of human bodies can be stimulated, natural secretion of growth hormone is promoted, pituitary cells are stimulated and repaired, a great quantity of osteoblasts can be generated continuously, and accordingly long bone length can be increased. The growth promoting preparation and the H-OL duplex height increasing system are mainly applied to height increase of men, women, teenagers, adults and people suffering from sexual precocity, nanism, dwarfism and developmental retardation and are excellent in curative effect.

The invention discloses a freshwater shrimp vitellogenin Vg gene, an encoding protein and an application of the freshwater shrimp vitellogenin Vg gene. The full-length cDNA sequence of the Vg gene is cloned from hepatopancreas of the freshwater shrimp by a primer group provided by the invention, and the expression of a vitellogenin gene in the ovary and the liver can be silenced after the dsRNA of the gene is injected to the pericardial cavity of a female freshwater shrimp, so that the ovary development of the freshwater shrimp is remarkably delayed. The invention provides a new thought and an effective means for solving the sexual precocity phenomenon of the freshwater shrimp and culturing good varieties.

The invention relates to an environment-friendly selenium-rich pig raising method. The method overcomes the problems that at present, in the live pig raising process, in order to avoid live pigs from being infected with diseases and make the live pigs grow fast, massive antibacterial agents are used from the suckling pig period until the pigs become full grown and ready for slaughter, so that antibiotic residues in pork are high and the quality of the pork is affected, harm is also brought to the physical fitness of people, especially, the harm to large number of adolescents is larger, and the adolescents are prone to sexual precocity. Aiming at the harm of the live pig raising method, the antibiotic-free and selenium-rich pig raising method and burdening are provided, and the health of the human body is ensured.

The invention provides a method for preparing hybrid triploid seeds of golden trout and arctic charr. The method includes: subjecting ova of female parent golden trout and sperms of male parent arctic charr to external fertilization, moving fertilized ova to a six-DMAP (N6-dimethyladenine) solution with concentration ranging from 170mg / L to 190mg / L and the temperature ranging from 9 DEG C to 11 DEG C after the fertilized ova are fertilized for 12-14 minutes, soaking for 14-16 minutes prior to moving into water at the temperature of 6-8 DEG C for hatching, and obtaining the hatched hybrid triploid larva fishes from the fertilized ova after about 50 days. During hatching, the eyeing rate of the fertilized ova is larger than 78%, the seed hatching rate reaches more than 90%, the obtained larva fishes are detected by a flow cytometer, and the triploid rate reaches 95%. The method for preparing the hybrid triploid seeds of the golden trout and the arctic charr remits the problems about sexual precocity, size miniaturization, disease breeding and the like of individuals during salmon and trout cultivation, and has the advantages of hybridization and triploid and a good marketing value.

The invention discloses an ecological breeding method of high-quality fancy carps. The method includes the following operating steps that 1, an aquaculture pond is prepared; 2, fertilization and waterplant planting are carried out; 3, sexual precocity freshwater shrimp fry are cultivated and delivered; 4, fancy carp fry are selected and delivered; 5, breeding management is carried out; 6, a fry replenishing measure is taken. The characteristics that sexual precocity freshwater shrimp individuals are small, the propagation time is about 15-20 days shorter than that of normal freshwater shrimps, and the rapid feeding growth periods of zoea and juvenile shrimps of the sexual precocity freshwater shrimps are completely consistent with those of the fancy carps are ingeniously utilized, the juvenile freshwater shrimps and adult freshwater shrimps can meet the requirements of the body type growth of the fancy carps on protein and the body color development on astaxanthin, the material flow relation between organisms of the aquaculture pond is creatively utilized fully, dynamic balance of material flow between the organisms is kept, and under the conditions without manual feeding strips and polluted water drainage, the high-quality fancy carps are bred. Meanwhile, the problem that during freshwater shrimp breeding, breeding economic benefits are affected due to sexual precocity of freshwater shrimps is solved, and the method is easy to operate and has remarkable economic, ecological and social effects.