The invention provides a cultivation method of large-specification juvenile eriocheir sinensis. The cultivation method comprises the steps of selecting a crab pool, regulating the water quality, planting alternanthera philoxeroides, selecting juvenile crabs, feeding the juvenile crabs, harvesting and the like. The breeding variety is 'Noah No. 1' of eriocheir sinensis, is a new variety of river crabs certificated by the National Aquatic Stock Seed and Fine Breed Certification Committee in 2016 and has the advantages of being high in growth speed, high in large-specification rate and the like.The planted aquatic plants are mainly alternanthera philoxeroides, alternanthera philoxeroides has a strong purifying capability, during high temperature in summer, the aquatic plants are not rotted,the water quality is not deteriorated, and it is guaranteed that the pool has good water quality in the breeding cycle. The specification of the put juvenile crabs is 140,000-160,000 per kg, 1.0-1.25kg of the juvenile crabs are put per mu, 200 kg or above of the juvenile crabs are harvested, the proportion of the large-specification juvenile crabs reaches 70% or above, and the problems that the juvenile crabs are subjected to sexual precocity, small in size, poor in quality and low in economic benefit are solved.