6 results about "Macrobrachium nipponense" patented technology

The invention discloses dsRNA for targeted regulation of a freshwater shrimp PKA-R1 gene and an obtaining method and application of the dsRNA, and belongs to the technical field of biology.Firstly, the freshwater shrimp PKA-R1 gene is obtained, then a gene segment with the sequence of SEQ ID NO: 3 is obtained through RNA interference and other technologies, dsPKA-R1 is synthesized through the gene segment, and after the synthesized dsPKA-R1 is injected into the pericardial cavity of a freshwater shrimp, it is proved that the dsPKA-R1 can slow down growth of the freshwater shrimp through growth parameter comparison.

This invention belongs to the field of shrimp farming technology and discloses an AIoT-driven ecological coupling method for whole-field flat-culture of fruit and shrimp. The method divides the fruit and flower crop planting area into multiple flat-culture units and establishes unit relationships. Under basic flat-culture management conditions, crop impact data, shallow water environment data, bottom load data, and shrimp activity data are collected to establish historical baseline states for each flat-culture unit. During the flat-culture process, current data is collected to form plant impact state, shallow water environment state, bottom load state, and shrimp response state, and a current ecological state chain is constructed. Based on the comparison results between the current ecological state chain and the historical baseline state, abnormal relationships are identified, and target flat-culture units, associated flat-culture units, and dominant abnormal links are determined. A local zonal control scheme is generated and executed. After control, a retested ecological state chain is formed, and recovery conditions are determined, dominant abnormal links are corrected, or the control level is increased accordingly.

Outdoor soil pond type culture of the brine shrimps is limited by seasons and the like, indoor factory pond culture is not influenced by seasons, and pond heating and oxygen pumping greatly increase the cost of culturing the brine shrimps. The method for breeding the brine shrimps can solve the problems of high cost, low yield, breeding region and season limitation and the like of breeding the brine shrimps.

The invention relates to the technical field of seedling raising, in particular to a method for increasing the survival rate of zoeae of macrobrachium nipponensis based on bacterium-membrane synergy, which comprises the following steps: S1, placing a porous carrier in a water body, and carrying out membrane hanging operation for 10-16 days to obtain a porous carrier loaded with microorganisms; s2, putting the porous carrier loaded with the microorganisms into a seedling culture device, putting macrobrachium nipponense seedlings into the seedling culture device, and performing water quality management and temperature control; s3, feeding different baits to different growth stages of the macrobrachium nipponensis larvae, and adding auxiliary materials. When macrobrachium nipponensis breeding is carried out, according to zoeae habit changes, the water quality can be remarkably improved, the stress resistance of the zoeae is enhanced, and the survival rate of breeding is increased by adopting the modes of porous biofilm culturing, bait feeding changing and the like.

The invention discloses a probe for marking gill tissue neuronal cells of macrobrachium hainanense and application of the probe, and belongs to the technical field of biology. The nucleotide sequence of the probe is as shown in SEQ ID NO. 1. According to the method, the probe is designed by screening and utilizing the specific high-expression gene AChE of the macrobrachium hainanense gill tissue neuronal cells for the first time, so that accurate in-situ labeling of the cells can be realized, and cross reaction with non-neuronal cells is effectively avoided. Through a fluorescence in situ hybridization (FISH) technical process, neuronal cells are mainly distributed in a gill axis, only a small part of neuronal cells are distributed in gill filaments, and an AChE gene expression signal is enhanced under alkalinity stress, which indicates that the gene can be used as an alkali-resistant molecular marker. According to the invention, the blank of the technology for marking the neuronal cells of the gill tissue of the crustacean is filled, an important genetic target and a technical means are provided for breeding a new strain of the high-alkali-resistant crustacean, and the method has a wide application prospect.

This invention belongs to the field of aquaculture technology, specifically relating to a plant-derived compound preparation for controlling precocious puberty in giant freshwater prawns, its preparation method, and its application method. The plant-derived compound preparation comprises the following raw materials in parts by weight: 1.5–3.0 parts gardenia extract, 0.5–1.2 parts quince extract, 0.5–1.0 parts mulberry leaf extract, 0.5–1.2 parts lily extract, 1.0–2.5 parts dried ginger extract, and 90–98 parts vitamin B complex powder. The plant-derived compound preparation is added to the feed of giant freshwater prawns at specific growth stages and simultaneously sprinkled into the aquaculture water. Through surface penetration and absorption, it regulates and improves hepatopancreatic function, enhances nutrient digestion and conversion, promotes growth, and reduces the probability of precocious puberty, thus replacing existing control measures. The technical solution of this invention has the characteristics of good and stable control effect, small dosage, convenient use, and high safety, ensuring the size and yield of marketable prawns, thereby achieving the goal of rationally controlling the density of giant freshwater prawns in ponds and improving aquaculture efficiency.