95 results about "SAA protein" patented technology

A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.

A method for producing protein compositions comprising fibrinogen and fibronectin is disclosed, wherein a fibrinogen and fibronectin-containing starting solution is treated with a precipitating composition which comprises two different components that modify the solubility of fibrinogen and/or fibronectin, so that in a single-step precipitation a precipitate is formed which comprises fibrinogen and fibronectin, and the precipitate formed optionally is further treated by methods known per se.

The invention provides methods of obtaining immunogenic proteins from genomic sequences including Neisseria, including the amino acid sequences and the corresponding nucleotide sequences, as well as the genomic sequence of Neisseria meningitidis B. The proteins so obtained are useful antigens for vaccines, immunogenic compositions, and/or diagnostics.

An object of the present invention is to provide a protein useful as a sphingomyelin detecting probe, which specifically recognizes sphingomyelin and has low cytotoxicity. The present invention provides a protein which has an amino acid sequence having, as the amino acid sequence from the 1st to the 48th amino acid, the amino acid sequence from the 1st to the 48th amino acid in Lysenin 1, and as the amino acid sequence from the 49th to the 298th amino acid, the amino acid sequence from the 51st to the 300th amino acid in Lysenin 3; and a protein which is obtained by deleting N terminal and/or C terminal from earthworm toxins Lysenin 1 or 3, and which specifically recognizes sphingomyelin.

The present invention relates to a mutant strain of Aspergillus sojae with an enhanced protease activity and a preparation method of a natural taste enhancer using the same. More specifically, the present invention relates to the mutant strain of Aspergillus sojae with an enhanced protease activity is obtained by selecting a strain with high protease activity and treating it with N-methyl-N′-Nitroso-N-nitrosoguanidine (NTG) and inducing a mutation through irradiating, and a preparation method of a natural taste enhancer using protein hydrolysate obtained by hydrolyzing the protein sources with their cultures.

Disclosed are methods of removing oil from an aqueous surface, comprising: surrounding an oil spot on the aqueous surface with an oil-absorbing material; and introducing a solution comprising a surfactant to the oil spot. Also disclosed are the above methods where the oil is not mechanically directed towards the oil-absorbing material, or where the oil-absorbing material is not mechanically directed towards the oil. Also disclosed are the above methods further comprising introducing a solution comprising a protein/surfactant complex to the oil spot, where the protein/surfactant complex comprises a protein component obtained from the fermentation of yeast, comprising a mixture of multiple intracellular proteins, at least a portion of the mixture including yeast polypeptides obtained from fermenting yeast and yeast stress proteins resulting from subjecting a mixture obtained from the yeast fermentation to stress.

The invention relates to the field of aqueous solutions comprising a protein. More specifically, the invention relates to the detection and/or removal of conformationally altered proteins and/or peptides comprising a cross-β structure from an aqueous solution comprising a protein. The invention provides methods for detecting and/or removing proteins and/or peptides comprising a cross-β structure from an aqueous solution comprising a protein, the method comprising contacting the aqueous solution comprising a protein with at least one cross-β structure-binding compound resulting in a bound protein or peptide with cross-β structure. The invention further provides a aqueous solution comprising a protein obtainable by a method of the invention, and a kit for carrying out the methods of the invention.

The present invention relates to a spider silk crystal protein gene synthesized by plant preferance codon, its expression carrier, the plant cells transformed from it, and the transgenic plant and its descendant. Said gene can be effectively expressed in fibrous plant and the obtained protein moleculae can be combined with the cellulose of plant, so improving the strength of plant fibres.

The present invention relates to a method for crystallization of a protein obtained from a protein-containing solution which involves (a) treating the protein-containing solution with a salt containing a sulphur atom having an oxidation state less than 6, and (b) recovering the protein in crystalline form.

The invention relates to a method for preparing microprotein, which takes active sludge generated during biochemical treatment of sewage and waste water, particularly remained active sludge from town sewage treatment plants, as raw materials. The method is characterized in that the active sludge is subjected to concentration regulation, hot basic hydrolysis, residue-liquid separation, filtrate condensation and concentrated solution drying to obtain protein products with different concentrations. The method has the advantages of simple technological process and equipment and easy industrialized production, and protein products prepared have the advantages of good foamability and foaming stability, high yield and low cost.

The invention relates to a method for preparing a protein molecular engram block polymer. Functional monomers, crosslinking agents and model protein molecules in proper proportions stand for 2 to 5 minutes at the temperature ranging from 0 to 4 DEG C, and a certain amount of evocating agents and rate accelerating materials are orderly added later. The mixture is put under the environment whose temperature ranges from minus 5 DEG C to minus 20 DEG C after being rapidly injected into a liquid chromatogram column jacket and polyreaction is completed within 1 to 12 hours. The model protein molecules are eluted by dodecyl sodium sulfate whose weight concentration is 10 percent and acetic acid solution whose volume concentration is 10 percent, namely, a macroporous protein molecular engram monolithic column can be prepared. The invention has the advantages as follows: the preparation method is simple and reliable, and the obtained protein molecular engram monolithic column has stronger template molecule recognition property. The technique provides a novel preparation method for developing protein molecular engram monolithic column.

A method for analyzing a structural affinity relationship between plural kinds of proteins and a compound, comprising the steps of (a) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of isotope-labeled proteins; (b) using a compound-immobilized carrier to purify plural kinds of proteins bound to the compound on the carrier, from a group of proteins brought into contact with a compound beforehand; (c) mixing the proteins obtained in step (a) and step (b); (d) analyzing the mixture obtained in step (c) with mass spectrometry; (e) identifying each of plural kinds of proteins based on information obtained by the mass spectrometry; and (f) obtaining an intensity ratio between a labeled peak and a non-labeled peak of each protein, thereby quantitating an affinity ratio of the compound to each protein.

The invention discloses anti-hepatitis B virus immunoglobulin of yolk and separation method. The separation method includes the following steps: animal immune; separating and purifying yolk; diluting by de-ionized water; adjusting pH; adding sodium chloride; setting for 5-6h; centrifugal separation; removing precipitation; adding sodium sulfate into supernatant to process the first salting out; centrifugalizing out protein precipitation; adding de-ionized water until the precipitation be fully dissolved; adding sodium sulfate to process the second salting out; centrifugal separation; the gained protein precipitation is added into de-ionized water until it is fully dissolved; filtering by gel-column; the eluted protein flow is monitored by ultraviolet photometer to absorb the 280 nm light wave and collect the first spectral band, concentrated by reversal dialysis, dialyzed to remove salt to gain the anti-hepatitis B virus IgY. The invention has the advantages of simple technology, low cost, and better sample quality.

Antibodies capable of recognizing many types of proteins having methyllysine residues are established by immunizing an animal with a chemically methylated protein other than histone and subsequent screening or the like depending on the reactivity to a protein obtained by chemically methylating a protein other than the protein used in the immunization. A process for producing such an antibody is also established. These antibodies are useful in searching for and studying various methylated proteins and are particularly useful in regulating the functions of biological molecules wherein the methylation of a lysine residue plays an important role, and in diagnosing a disease by detecting a methyllysine-containing protein.

The invention discloses an SAA monoclonal antibody 03-7-3, the heavy chain of the SAA monoclonal antibody 03-7-3 is IgG1, the light chain is k, and the amino acid sequences of the variable regions ofthe heavy chain and the light chain are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The SAA monoclonal antibody 03-7-3 disclosed by the invention can specifically identify disease-related SAA-ApoA1-HDL complex and SAA proteins, and on the basis of the SAA monoclonal antibody 03-7-3, an assay method for the SAA-ApoA1-HDL complex and the SAA proteins is established. The SAA-ApoA1-HDL complex is significantly associated with liver cirrhosis, and is a potential risk factor for the liver cirrhosis; and the concentration of the SAA proteins is significantly associated with the liver cirrhosis due to hepatitis B and liver cancer, and is a potential risk factor for the liver cirrhosis due to hepatitis B and the liver cancer. Therefore, the SAA monoclonal antibody 03-7-3 disclosed by the invention has important clinical value for the prediction of the potential risk of the liver cirrhosis and the liver cancer and the development of diagnostic markers for the diseases.

The invention discloses a serum amyloid protein A mutant which has an amino acid sequence as shown in SEQ ID NO.1 and still has antigenicity of serum amyloid protein after several amino acids are replaced, so that a unique mutant is obtained. The SAA mutant is obtained by replacing four amino acids in natural SAA protein, so that the in-vitro stability of the SAA mutant is improved. Experiments prove that obtained SAA protein is soluble mature protein, the activity of the SAA protein is kept to the maximum extent, and the antigenicity of natural SAA protein is reserved. The invention also discloses application and a preparation method of the serum amyloid protein A mutant.