11 results about "SAA protein" patented technology

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination capable of simultaneously recognizing equine and feline serum amyloid A (SAA) and its application. The monoclonal antibody combination comprises 6E10 and 5A6. The CDR sequences of the heavy and light chain variable regions of 6E10 are shown in SEQ ID NO. 1-6, and the CDR sequences of the heavy and light chain variable regions of 5A6 are shown in SEQ ID NO. 7-12. This antibody combination can highly specifically recognize and bind to recombinant equine and feline SAA proteins, achieving multiple uses with a single antibody and reducing raw material development and production costs. The colloidal gold test strip constructed based on this combination exhibits high specificity, high sensitivity, and a wide detection range, with no cross-reactivity. It is easy to operate and requires no complex instruments, providing a stable and reliable biorecognition tool for assessing and monitoring inflammation-related physiological states in animals.

The invention relates to a characteristic peptide fragment group for SAA protein valuing and application of the characteristic peptide fragment group. The characteristic peptide fragment group comprises a sequence 1, a sequence 1 of an I site connected isotope, a sequence 2 and a sequence 2 of an F site connected isotope, the amino acid sequence of the sequence 1 comprises a sequence as shown in SEQ ID NO.1, and the amino acid sequence of the sequence 2 comprises a sequence as shown in SEQ ID NO.2. According to the method, the isotope labeled characteristic peptide fragment is introduced as an internal standard, so that the loss in the sample treatment process and the signal fluctuation in the mass spectrometric detection process are effectively corrected, and the influence of a matrix effect on a quantitative result is overcome, so that the quantitative accuracy of the SAA protein is remarkably improved.

Provided is an immunostimulatory preparation which is a protein obtained by fusing a bacteria-derived heat shock protein (HSP: heat shock protein) and a viral peptide antigen, and enables induction of a highly diverse immunoglobulin by simultaneously stimulating nasal, respiratory, and oral administration. Provided are a feed, a feed additive, and an environmental symbiotic preparation for livestock, or a cosmetic, a food, and a quasi-pharmaceutical product for humans, which enable symbiosis with an environmental microorganism containing the immunostimulatory preparation. Provided are a preparation, a cosmetic for humans, a food, and a quasi-pharmaceutical product that enable symbiosis with environmental microorganisms utilizing livestock-derived IgA, IgG, and IgY among the immunoglobulins.

The invention provides a protein function annotation method and device, electronic equipment and a storage medium, and the method comprises the steps: respectively obtaining a sequence feature, a pre-training sequence feature and a preset annotation feature according to a protein sequence; performing feature extraction processing on the preset annotation feature to obtain a first learning feature; performing feature extraction processing on the sequence features and the pre-training sequence features to obtain second learning features; performing fusion processing on the first learning feature and the second learning feature to obtain a first fusion feature; and processing the first fusion feature to obtain protein function annotation information. Compared with the prior art, the method has the advantages that the sequence feature, the pre-training sequence feature and the preset annotation feature are obtained based on the protein sequence, and the obtained protein function annotation information is more accurate through fusion processing of the three features from different angles.

The present application relates to a kind of novel protein and its application specially combined with small molecule HBC620 and induced its fluorescence enhancement.The amino acid sequence of the protein is as shown in SEQ ID NO:1.The protein obtained in the present application can effectively reduce the non-specific binding with other structure similar small molecules due to the directional design of binding pocket around HBC620, thereby reducing the background fluorescence interference, improving detection accuracy and system stability.

The present disclosure is based on the surprising and unexpected discovery that a ligand molecule with certain characteristics is able to bind to two protein molecules simultaneously and recruit them to form a transient or stable protein-protein interaction complex. The protein-protein interaction and other cross-domain interactions gained in this process contribute additional stabilization energy to the complex beyond the combination of the binary binding energies, and therefore, largely increase the binding potency of the ligand. Accordingly, the present disclosure provides a Protein-Protein Interaction Inducing Technology (PPIIT), which includes a method to design and identify the tripartite or bifunctional compounds and use such compounds to induce protein-protein interactions in various contexts. The present disclosure also provides a composition for the purpose of inducing protein-protein interactions.

Provided are a method for dissolving a poorly water-soluble protein, and a molded article produced using a protein solution obtained thereby. The present invention is a method for dissolving a poorly water-soluble protein, the method comprising a step for mixing a hydroxyl group-containing polymer, a poorly water-soluble protein, and an ionic liquid. In addition, the present invention makes it possible to produce a molded article using the poorly water-soluble protein solution obtained by the method.

The present invention provides a computer-implemented method for determining if a pancreatic cyst of a subject is pre-malignant / malignant or benign, the method comprising a) providing protein abundance data obtained from a liquid sample from the subject ("sample data"); b) providing the sample data as input to a machine learning model trained on a training data set comprising a protein abundance profile of at least three pancreatic carcinomas and at least three presumed benign pancreatic cysts, the protein abundance profile comprising the abundance level of at least 15 proteins selected from Table 1 ("training data"), wherein each pancreatic carcinoma is a distinct pancreatic carcinoma subtype and each presumed benign pancreatic cyst is a distinct presumed benign pancreatic cyst subtype; c) receiving an output from the machine learning model, the output indicative of a pre-malignant / malignant classification or a benign pancreatic cyst classification for the sample data; and d) determining if the pancreatic cyst of the subject is pre-malignant / malignant or benign based on the output received in step c). Related methods and systems for use in performing the methods are also provided.

This invention discloses a recombinant serum amyloid A (r) protein from the Pacific oyster. Cg SAA), preparation method and application, r Cg The SAA amino acid sequence is shown in SEQ ID NO. 1; the preparation method is to use primers P1 and P2 to... Cg The SAA coding region fragment was amplified by PCR, and the DNA sequences of primers P1 and P2 are shown in SEQ ID NO. 2 and SEQ ID NO. 3, respectively. The PCR amplification product was then mixed with the pET-30a vector via... Bam HI and Xhol After digestion with enzyme I, ligation was performed using T4 ligase, followed by transformation to construct recombinants; the recombinants were then transformed into *E. coli*. Transetta In the (DE3) expression strain, the expression strain was then cultured and IPTG was added to induce expression, followed by purification and refolding to obtain r. Cg SAA protein. The prepared r Cg SAA inhibits the growth of Vibrio splenti and Vibrio anguillarum under non-acidic conditions, and can also kill Vibrio splenti. It can be used to prepare drugs against Vibrio splenti and Vibrio anguillarum, and can be applied directly, effectively and safely to aquaculture.

The application provides a SAA recombinant protein and a preparation method and application thereof, and the preparation method comprises the following steps: S10, constructing a recombinant plasmid: a gene sequence of an amino acid sequence shown in SEQ ID NO: 1 or a nucleotide sequence corresponding to an amino acid sequence shown in SEQ ID NO: 2 is inserted into a pET-28a(+) plasmid which is double-digested by NdeI and XhoI after being double-digested by NdeI and XhoI, so that a recombinant plasmid is obtained; S20, transforming and culturing the recombinant plasmid; S30, protein expression and purification. The application solves the technical problem that the stability of the currently recombinantly expressed SAA protein in vitro is poor, and achieves the technical effect of improving the stability of the SAA recombinant protein in vitro.

The invention relates to a serum amyloid A (SAA) protein mutant and a preparation method thereof. Specifically, the serum amyloid A protein mutant contains core amino acids selected from the following groups: (a) the first amino acid is A or G; (b) the fifth amino acid is A or G; and / or (c) the 8th amino acid is A; wherein the amino acid position number is based on the sequence represented by any one of SEQ ID NO: 1 and SEQ ID NO: 69 to SEQ ID NO: 73. According to the method, the yield of SAA protein is increased, the problem of endotoxin is avoided, and the difference of folding modes or immunogenicity when other systems are used for expressing protein is avoided.