21 results about "SAA protein" patented technology

Provided are a new drug candidate discovery system and a computer program implementing a new drug candidate discovery platform. A new drug candidate discovery system may include an automatic data preprocessing module configured to receive a target protein information from a user through a web interface, and perform preprocessing on a protein structure file obtained based on the target protein information; a simulation setting module configured to predict an Enzymatically Active Pocket for Docking Calculation (EAPDC) from the protein structure file using an artificial intelligence language model, and determine a docking calculation site; and a docking simulation module configured to perform a docking simulation for the docking calculation site.

The invention relates to methods for the preparation of protein hydrolysates having low concentrations of undesired nitrosamines as well as the thus obtained protein hydrolysates, compositions containing them and uses thereof. The methods comprise the steps of (a) contacting at least one protein with at least one oxidant, preferably a peroxide, at a concentration of 10 to <500 ppm relative to the weight of the at least one protein, at a pH in the range of 2.0 to <7.0; and (b) subjecting the at least one protein of step (a) to a hydrolysis reaction to obtain a protein hydrolysate.

The invention discloses an active tuberculosis diagnosis method based on proteomics and metabonomics. The method comprises the following steps: collecting a serum sample of a suspected active tuberculosis patient; detecting specific proteins in the serum sample by using a proteomics technology; detecting specific amino acids in the serum sample by using a metabonomics technology; carrying out standardization treatment on the obtained protein, amino acid and bile acid data; establishing a diagnosis model by using a machine learning algorithm; inputting data of a to-be-diagnosed sample into the model, and calculating a probability value of the to-be-diagnosed sample suffering from active tuberculosis; according to a preset threshold value, whether the sample provider suffers from the active tuberculosis or not is judged. By comprehensively analyzing multiple omics data, the sensitivity and specificity of diagnosis are greatly improved; by utilizing a machine learning algorithm, the disease risk can be objectively and quantitatively evaluated, and human misjudgment is reduced; the detection process is simple and fast, the sample demand is small, and clinical popularization and application are facilitated.

Disclosed herein are food products comprising a non-animal-origin protein and methods for producing the food products, wherein the non-animal-origin protein may not be a protein or a fragment or truncation of a protein obtained from an animal.

The present invention discloses a bispecific antibody or a functional fragment thereof of a cat SAA protein, comprising: a first functional domain and a second functional domain that specifically recognize and bind to a cat SAA protein, the amino acid sequences of VH CDR1, CDR2, CDR3, VL CDR1, CDR2 and CDR3 of the first functional domain are shown in SEQ ID NOs: 1 to 6, respectively, and the amino acid sequences of VH CDR1, CDR2, CDR3, VL CDR1, CDR2 and CDR3 of the second functional domain are shown in SEQ ID NOs: 7 to 12, respectively. The present invention also discloses the use of a bispecific antibody or a functional fragment thereof of a cat SAA protein in preparing a drug or a kit for evaluating the degree of inflammation and tissue damage and the prognosis of a cat. The present invention can rapidly and dynamically monitor the concentration of SAA in cat plasma, and has important indicative significance for early diagnosis and treatment of inflammation and tissue damage in cats.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination capable of simultaneously recognizing equine and feline serum amyloid A (SAA) and its application. The monoclonal antibody combination comprises 6E10 and 5A6. The CDR sequences of the heavy and light chain variable regions of 6E10 are shown in SEQ ID NO. 1-6, and the CDR sequences of the heavy and light chain variable regions of 5A6 are shown in SEQ ID NO. 7-12. This antibody combination can highly specifically recognize and bind to recombinant equine and feline SAA proteins, achieving multiple uses with a single antibody and reducing raw material development and production costs. The colloidal gold test strip constructed based on this combination exhibits high specificity, high sensitivity, and a wide detection range, with no cross-reactivity. It is easy to operate and requires no complex instruments, providing a stable and reliable biorecognition tool for assessing and monitoring inflammation-related physiological states in animals.

The invention relates to a characteristic peptide fragment group for SAA protein valuing and application of the characteristic peptide fragment group. The characteristic peptide fragment group comprises a sequence 1, a sequence 1 of an I site connected isotope, a sequence 2 and a sequence 2 of an F site connected isotope, the amino acid sequence of the sequence 1 comprises a sequence as shown in SEQ ID NO.1, and the amino acid sequence of the sequence 2 comprises a sequence as shown in SEQ ID NO.2. According to the method, the isotope labeled characteristic peptide fragment is introduced as an internal standard, so that the loss in the sample treatment process and the signal fluctuation in the mass spectrometric detection process are effectively corrected, and the influence of a matrix effect on a quantitative result is overcome, so that the quantitative accuracy of the SAA protein is remarkably improved.

Provided is an immunostimulatory preparation which is a protein obtained by fusing a bacteria-derived heat shock protein (HSP: heat shock protein) and a viral peptide antigen, and enables induction of a highly diverse immunoglobulin by simultaneously stimulating nasal, respiratory, and oral administration. Provided are a feed, a feed additive, and an environmental symbiotic preparation for livestock, or a cosmetic, a food, and a quasi-pharmaceutical product for humans, which enable symbiosis with an environmental microorganism containing the immunostimulatory preparation. Provided are a preparation, a cosmetic for humans, a food, and a quasi-pharmaceutical product that enable symbiosis with environmental microorganisms utilizing livestock-derived IgA, IgG, and IgY among the immunoglobulins.

The invention provides a protein function annotation method and device, electronic equipment and a storage medium, and the method comprises the steps: respectively obtaining a sequence feature, a pre-training sequence feature and a preset annotation feature according to a protein sequence; performing feature extraction processing on the preset annotation feature to obtain a first learning feature; performing feature extraction processing on the sequence features and the pre-training sequence features to obtain second learning features; performing fusion processing on the first learning feature and the second learning feature to obtain a first fusion feature; and processing the first fusion feature to obtain protein function annotation information. Compared with the prior art, the method has the advantages that the sequence feature, the pre-training sequence feature and the preset annotation feature are obtained based on the protein sequence, and the obtained protein function annotation information is more accurate through fusion processing of the three features from different angles.

The present invention relates to genetic engineering, biotechnology, immunology, and microbiology. Recombinant GBD-SSTad-SSTad protein and a method for preparation thereof on glucan are described, including the binding of GBD-SSTad-SSTad protein in cell extracts of E. coli BL21 [pGBD-SSTad-SSTad] strain to an alpha-glycan-containing sorbent due to affinity interaction during the incubation procedure, subsequent washing from unbound bacterial proteins and isolation of the desired product. The invention also relates to an immunogenic composition and a kit comprising said peptide. The invention also relates to the use of the obtained protein to increase the number of maturing follicles and improve the quality of sperm. This technical result, an increase in the number of maturing follicles and an improvement in the quality of sperm is based on the use of the KNFFWKTFTS peptide, which is part of a recombinant protein that is capable of eliciting autoantibodies to somatostatin.

The invention relates to a method for extracting a plant protein, comprising the following steps: 1. Suspending a plant powder in a preferential aqueous solvent in order to obtain a plant powder suspension; 2a, adding a strain into the plant powder suspension in the step 1; 2b. Incubating the inoculated suspension of step 2a such that the strain acidifies the inoculated suspension until a protein precipitate is formed; 3. Separating the protein precipitate obtained at the end of step 2b; and the invention relates to a plant protein isolate obtainable by the method of the invention.

The present application relates to a kind of novel protein and its application specially combined with small molecule HBC620 and induced its fluorescence enhancement.The amino acid sequence of the protein is as shown in SEQ ID NO:1.The protein obtained in the present application can effectively reduce the non-specific binding with other structure similar small molecules due to the directional design of binding pocket around HBC620, thereby reducing the background fluorescence interference, improving detection accuracy and system stability.

The present disclosure is based on the surprising and unexpected discovery that a ligand molecule with certain characteristics is able to bind to two protein molecules simultaneously and recruit them to form a transient or stable protein-protein interaction complex. The protein-protein interaction and other cross-domain interactions gained in this process contribute additional stabilization energy to the complex beyond the combination of the binary binding energies, and therefore, largely increase the binding potency of the ligand. Accordingly, the present disclosure provides a Protein-Protein Interaction Inducing Technology (PPIIT), which includes a method to design and identify the tripartite or bifunctional compounds and use such compounds to induce protein-protein interactions in various contexts. The present disclosure also provides a composition for the purpose of inducing protein-protein interactions.

Provided are a method for dissolving a poorly water-soluble protein, and a molded article produced using a protein solution obtained thereby. The present invention is a method for dissolving a poorly water-soluble protein, the method comprising a step for mixing a hydroxyl group-containing polymer, a poorly water-soluble protein, and an ionic liquid. In addition, the present invention makes it possible to produce a molded article using the poorly water-soluble protein solution obtained by the method.

The present invention provides a computer-implemented method for determining if a pancreatic cyst of a subject is pre-malignant / malignant or benign, the method comprising a) providing protein abundance data obtained from a liquid sample from the subject ("sample data"); b) providing the sample data as input to a machine learning model trained on a training data set comprising a protein abundance profile of at least three pancreatic carcinomas and at least three presumed benign pancreatic cysts, the protein abundance profile comprising the abundance level of at least 15 proteins selected from Table 1 ("training data"), wherein each pancreatic carcinoma is a distinct pancreatic carcinoma subtype and each presumed benign pancreatic cyst is a distinct presumed benign pancreatic cyst subtype; c) receiving an output from the machine learning model, the output indicative of a pre-malignant / malignant classification or a benign pancreatic cyst classification for the sample data; and d) determining if the pancreatic cyst of the subject is pre-malignant / malignant or benign based on the output received in step c). Related methods and systems for use in performing the methods are also provided.

This invention discloses a recombinant serum amyloid A (r) protein from the Pacific oyster. Cg SAA), preparation method and application, r Cg The SAA amino acid sequence is shown in SEQ ID NO. 1; the preparation method is to use primers P1 and P2 to... Cg The SAA coding region fragment was amplified by PCR, and the DNA sequences of primers P1 and P2 are shown in SEQ ID NO. 2 and SEQ ID NO. 3, respectively. The PCR amplification product was then mixed with the pET-30a vector via... Bam HI and Xhol After digestion with enzyme I, ligation was performed using T4 ligase, followed by transformation to construct recombinants; the recombinants were then transformed into *E. coli*. Transetta In the (DE3) expression strain, the expression strain was then cultured and IPTG was added to induce expression, followed by purification and refolding to obtain r. Cg SAA protein. The prepared r Cg SAA inhibits the growth of Vibrio splenti and Vibrio anguillarum under non-acidic conditions, and can also kill Vibrio splenti. It can be used to prepare drugs against Vibrio splenti and Vibrio anguillarum, and can be applied directly, effectively and safely to aquaculture.

The application provides a SAA recombinant protein and a preparation method and application thereof, and the preparation method comprises the following steps: S10, constructing a recombinant plasmid: a gene sequence of an amino acid sequence shown in SEQ ID NO: 1 or a nucleotide sequence corresponding to an amino acid sequence shown in SEQ ID NO: 2 is inserted into a pET-28a(+) plasmid which is double-digested by NdeI and XhoI after being double-digested by NdeI and XhoI, so that a recombinant plasmid is obtained; S20, transforming and culturing the recombinant plasmid; S30, protein expression and purification. The application solves the technical problem that the stability of the currently recombinantly expressed SAA protein in vitro is poor, and achieves the technical effect of improving the stability of the SAA recombinant protein in vitro.

The present invention discloses a method for predicting the acid-base stability of proteins, an electronic device, and a storage medium. The method includes: (1) collecting bacteria, the tolerated pH values for their survival and tolerance, and the exoprotein sequences of the corresponding bacteria; (2) using the tolerated pH value of the bacteria as the tolerated pH value of the exoproteins of the bacteria to obtain protein sequence and its tolerated pH value data; (3) using the protein sequence and its tolerated pH value data as training samples to train a classification model or a regression model to obtain a trained protein acid-base stability prediction model; (4) inputting the protein sequence whose acid-base stability is to be predicted into the protein acid-base stability prediction model obtained in step (3) to obtain a protein acid-base stability prediction result. By using the tolerated pH value of the bacteria as the tolerated pH value of the exoproteins of the bacteria, the present invention obtains protein sequence and its tolerated pH value data for predicting the acid-base stability of exoproteins and intracellular proteins.

The present invention discloses a ZmWAK-RLK protein related to gray leaf spot resistance, and an encoding gene and application thereof. The present invention sets forth a protein obtained from a maize inbred line Y32, named ZmWAK-RLK, and being the protein shown in sequence 1 of the sequence listing. A nucleic acid molecule for encoding the ZmWAK-RLK protein is likewise considered to be within the scope of protection of the present invention. The present invention likewise sets forth a method for preparing a transgenic plant, comprising the following step: introducing said nucleic acid molecule into a starting plant, thus obtaining a transgenic plant having enhanced resistance to gray leaf spot. The present invention additionally sets forth a plant breeding method, comprising the following step: increasing the content and / or activity of the ZmWAK-RLK protein in a plant of interest, thus increasing the gray leaf spot resistance of the plant of interest. The present invention has great value when applied to the breeding of maize resistant to gray leaf spot.

The invention relates to a serum amyloid A (SAA) protein mutant and a preparation method thereof. Specifically, the serum amyloid A protein mutant contains core amino acids selected from the following groups: (a) the first amino acid is A or G; (b) the fifth amino acid is A or G; and / or (c) the 8th amino acid is A; wherein the amino acid position number is based on the sequence represented by any one of SEQ ID NO: 1 and SEQ ID NO: 69 to SEQ ID NO: 73. According to the method, the yield of SAA protein is increased, the problem of endotoxin is avoided, and the difference of folding modes or immunogenicity when other systems are used for expressing protein is avoided.

The present disclosure provides a protein functional annotation method and apparatus, an electronic device, and a storage medium. The method comprises: separately acquiring a sequence feature, a pre-trained sequence feature, and a preset annotation feature on the basis of a protein sequence; performing feature extraction on the preset annotation feature to obtain a first learning feature; performing feature extraction on the sequence feature and the pre-trained sequence feature to obtain a second learning feature; fusing the first learning feature and the second learning feature to obtain a first fused feature; and processing the first fused feature to obtain protein functional annotation information. Compared with the related art, the present disclosure relates to acquiring a sequence feature, a pre-trained sequence feature, and a preset annotation feature on the basis of a protein sequence, and fusing the three different aspects of features, such that protein functional annotation information obtained is more accurate.