16 results about "Ligand molecule" patented technology

The objective is to provide a novel method for improving the efficiency of osteogenic differentiation induction based on immobilized BMP-2. [Solution] A method for stimulating cells with ligand molecules, comprising a stimulation step of applying physical stimulation to the cells and / or ligand molecules, wherein the ligand molecule is bound to or interacts with a receptor protein expressed on the cells and is immobilized on a culture substrate and / or matrix molecule used for culturing the cells, the receptor protein mediates the transmission of extracellular signals to the cells based on its binding to the ligand molecule, and the extracellular signals are enhanced by the physical stimulation.

The present invention addresses the problem of providing a novel method for increasing bone differentiation induction efficiency based on immobilized BMP-2.　Provided is a method for stimulating a cell with a ligand molecule, the method including a stimulation step for applying a physical stimulus to the cell and / or the ligand molecule. The ligand molecule binds to or interacts with a receptor protein expressed on the cell, and is immobilized on a culture substrate and / or a matrix molecule used for culturing the cell. The receptor protein mediates the transmission of an extracellular signal to the cell on the basis of binding to the ligand molecule, and the extracellular signal is enhanced by the physical stimulus.

This invention discloses an EGCG supramolecular compound with antioxidant, liver-protective, and fat-reducing effects, its preparation method, and composition. The supramolecular compound is formed by non-covalent bonding between epigallocatechin gallate (EGCG) and ligand molecules containing trimethylamine and carboxyl groups; the supramolecular compound has a co-amorphous structure; the ligand molecules containing trimethylamine and carboxyl groups are betaine, ergothioneine, or L-carnitine. This invention can solve the problems of low stability and bioavailability of EGCG. It can significantly improve the solubility of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine, reduce the hygroscopicity of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine, and enhance the stability and bioavailability of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine.

The application belongs to the technical field of separation and extraction, and particularly relates to a 8C-DAPhen ligand, a preparation method and application thereof. The method is developed from the design of a ligand molecular structure, and a novel cyclic DAPhen ligand is developed through specific structure design. The ligand has a separation factor (SF Eu / Am ) of more than 600 for lanthanide and actinide elements, and exhibits excellent separation performance. The application provides a new perspective for in-depth understanding of lanthanide and actinide separation mechanism, and provides technical support for establishing an advanced spent nuclear fuel reprocessing system and realizing efficient utilization of minor actinide elements.

This invention provides a liver-targeting glycoligand molecule modified with dual-antenna GalNAc and its drug delivery system. This liver-targeting glycoligand molecule and its drug delivery system, through ASGPR recognition, can maximize the concentration of therapeutic drugs in liver tumor parenchymal cells, thereby improving the targeting of drug distribution, increasing the therapeutic index, reducing systemic toxicity, and improving patient survival time and quality of life. The liver-targeting glycoligand molecule of this invention is synthesized using an enzymatic synthesis method, which involves fewer synthesis steps, mild enzymatic reaction conditions, high regioselectivity, high reaction efficiency, is environmentally friendly, and has low production costs, making it highly promising for industrialization.

This invention discloses a bivalent ligand molecule targeting EGFR and its applications, belonging to the field of drug development technology. Its general structural formula is: [Formula omitted for brevity], where L is a linking group, and M1 and M2 are EGFR protein ligands. This invention forms a bivalent EGFR ligand molecule by covalently linking two EGFR ligands through a linking group. This bivalent ligand molecule can induce additional protein-protein interactions between EGFR monomers, which greatly enhances the binding strength and stability of the drug to EGFR, thereby overcoming the drug resistance problem of traditional EGFR inhibitors and providing a new treatment strategy for cancer patients carrying EGFR mutations and other patients with other diseases.

The application discloses a DNA aptamer binding to LOX-1 protein and application thereof. Specifically, the application provides a DNA aptamer binding to LOX-1 protein, wherein a core sequence of the DNA aptamer binding to the LOX-1 protein comprises a sequence as shown in SEQ ID NO: 5. The aptamer has small molecular weight, stable chemical property, is easy to preserve and label, can be combined with the LOX-1 protein through space conformation matching, accumulation of bases in a sequence, electrostatic interaction between charged groups or hydrogen bond interaction, and the like, has high affinity and good specificity.

This invention relates to the field of molecular generation technology, and discloses a method for constructing a molecular generation model and a molecular generation method. The method includes: acquiring a multimodal sample dataset, which comprises several protein sample groups. Each protein sample group includes pocket structure information of the target protein, ligand molecule information binding to the target protein pocket, textual description information of the target protein pocket, and molecular fragment constraint information; training a language learning model based on the multimodal sample dataset to obtain a molecular generation model. This invention enables the model to fully integrate multi-dimensional conditional information within a unified training framework, effectively improving the model's control over the molecular generation process and the accuracy of structure prediction. The generated molecules can efficiently bind to the target protein pocket while meeting semantic requirements and structural constraints, effectively adapting to the complex and multi-constrained design requirements in real-world drug development.

This invention discloses a molecular generation method based on a variable flow model using protein structure, comprising the following steps: Step 1: Obtain the pdb format file of the protein receptor for which ligand molecules need to be generated; Step 2: Assuming the protein receptor for which ligand molecules need to be generated comprises p atoms, extract the atom type features and atom spatial position features of the protein receptor; the atom type features are feature vectors of size p×1, and the atom spatial position features are feature vectors of size p×3; the atom spatial position includes: distance, bond angle, and dihedral angle; Step 3: Input the extracted atom type features and atom spatial position features of the protein receptor into a pre-trained molecular generation model GraphSF to generate several atoms; Step 4: Add bonds according to the interatomic distances, and finally output the corresponding ligand molecule in SDF format.

The application discloses a high-quality tin-based perovskite film and a preparation method and a photoelectric device thereof, and the preparation method comprises the following steps: dissolving ligand molecules R1 and a divalent tin salt in a solvent and stirring to obtain a first solution, wherein the ligand molecules R1 contain imidazole groups or carbene groups with a large steric effect; adding a reducing agent capable of replacing zero-valent tin atoms into the first solution, and fully reacting to obtain a second solution; separating and purifying the second solution to obtain a single-atom zero-valent tin complex; adding the single-atom zero-valent tin complex into a tin-based perovskite precursor solution to obtain a tin-based perovskite precursor solution with an additive; and coating the tin-based perovskite precursor solution with the additive on a clean substrate surface to obtain the high-quality tin-based perovskite film. The single-atom zero-valent tin complex can effectively induce Sn 4+ ion reduction to Sn 2+ , and can slow down the tin-based perovskite crystallization process, thereby effectively improving the comprehensive performance of the tin-based perovskite photoelectric device.

The application belongs to the technical field of extraction separation, and particularly relates to an OE-DAPhen ligand, a preparation method and application thereof. The application develops a novel DAPhen ligand through specific structure design from the design of a ligand molecular structure. The separation factor (SF) of the OE-DAPhen ligand for lanthanide and actinide elements is more than 400, and the OE-DAPhen ligand exhibits excellent separation performance. The application provides a new perspective for in-depth understanding of lanthanide and actinide separation mechanism, and provides technical support for establishing an advanced spent nuclear fuel reprocessing system and realizing efficient utilization of minor actinide elements. Eu / Am ).

The present application relates to a kind of catalytic system for olefin hydroformylation reaction and the method of olefin hydroformylation reaction;Catalytic system includes: fluorine-containing bisphosphinite ligand Ln and rhodium-manganese bimetallic synergistic system.The present application also relates to the preparation method of fluorine-containing bisphosphinite ligand Ln.The fluorine-containing bisphosphinite ligand of the present application is creatively introduced, and its fluorine-containing structure can significantly enhance the chemical inertness of ligand molecule itself, effectively resist decomposition, hydrolysis and degradation under high temperature.The introduction of manganese can not only adjust the catalytic performance of rhodium center through electronic effect, but also can form synergistic effect with rhodium in the reaction, and stabilize the key reaction intermediate, so as to enhance the firmness of the entire catalytic framework at the molecular level, further inhibit the precipitation and agglomeration of rhodium metal and inactivation.In propylene hydroformylation reaction, the conversion rate is generally as high as 99%;In the wide process parameter interval, the conversion rate is not less than 96%, the activity window is wide, and it has great industrial application prospect and economic benefit.

This invention belongs to the field of molecular biology and drug screening technology, and provides a method for screening capsaicin targeting SOCS5-RBMX protein interactions and its application. The method involves analyzing the structure of the SOCS5-RBMX protein complex to determine the SOCS5-RBMX binding domain and key binding sites; verifying the inhibitory effect on protein binding through point mutations at these key sites; identifying the binding pocket; using drugs from the ZINC22 small molecule drug database and FDA-approved drugs as ligand molecules, and performing virtual screening with the binding pocket as the docking region to obtain compounds; screening the obtained compounds using AMDET to identify capsaicin as the drug inhibiting SOCS5-RBMX binding; and further screening and verification using capsaicin in in vivo and in vitro experiments. This invention, through the analysis of the SOCS5-RBMX protein complex structure to determine the binding domain and key sites, and then using this as a basis for virtual screening and experimental verification, can accurately screen for drugs inhibiting SOCS5-RBMX binding, improving the accuracy and efficiency of drug screening.

The embodiment of the present disclosure provides a light emitting diode device, a display panel, a display device and a manufacturing method. The light emitting diode device comprises a substrate (1), a first electrode (2) located on one side of the substrate (1), a carrier functional layer (3) located on the side of the first electrode (2) away from the substrate (1), a quantum dot light emitting layer (4) located on the side of the carrier functional layer (3) away from the first electrode (2), and the quantum dot light emitting layer (4) has a molecular chain structure inside, wherein the molecular chain structure is formed by an atom transfer radical polymerization reaction of a first reactant and a modified molecule, the first reactant is subjected to an atom transfer radical polymerization reaction with the modified molecule through a ligand molecule A, and the modified molecule is in an initial state of being connected to one side of the carrier functional layer (3) facing the quantum dot light emitting layer (4); and a second electrode (5) located on the side of the quantum dot light emitting layer (4) away from the carrier functional layer (3).

The application relates to a preparation method and application of a dopamine self-polymerization under a magnetic field to form a fluorescence / colorimetric dual-mode sensor of aptamer-molecular imprinting synergic recognition, which comprises the following steps: step 1, preparing zirconium-containing metal organic framework material UiO-66-NH2; step 2, preparing aptamer-modified zirconium-containing metal organic framework material Apt-UiO-66-NH2; step 3, mixing UiO-66-NH2-Apt, a virtual template agent, a crosslinking agent dopamine and a buffer solution, and then polymerizing under a magnetic field, and then eluting the template agent, so that the sensor is obtained. The dopamine polymerization process is optimized by introducing a magnetic field, local excessive crosslinking is avoided, uniform and efficient specific recognition sites are formed, the prepared sensor combines the high affinity of the aptamer and the high stability of the molecular imprinting, and dual-mode signal output is realized by using the peroxidase activity of the MOF. The sensor has wide linear range, low detection limit, good specificity and excellent reproducibility, and can be used for rapid and high-sensitivity detection of fungal toxins in food.