302 results about "Ligand molecule" patented technology

The present invention relates to viruses that are engineered to contain a surface ligand molecule which targets the virus to a cell of interest. In particular non-limiting embodiments, the cell of interest is desirably ablated and may be a cancer cell, an infected cell, a cell exhibiting a non-malignant proliferative disorder, or a cell of the immune system. Alternatively, the cell of interest is a target for gene therapy.

A method for manufacturing an array of biomolecules that have complementary binding species is disclosed. The method utilize a master substrate containing a desired master array of binding molecules or ligands, e.g., DNA target molecules. A mixture of complementary, antiligand molecules, e.g., DNA probe molecules, are then hybridized to the ligands of the master array, Forming an array of antiligand molecules reversibly bound to the molecules in the master array. The antiligand array is then transferred and bound to a separate substrate, to form a desired array of antiligand molecules on the separate substrate. The process may be repeated to produce multiple arrays or multiple generations of arrays. Also disclosed is a sensor array device and method. The device includes an array of vibratory tympanic elements, each having a specific analyte binding probe carried on the vibratory membrane of the element. A binding event is detected by a change in the vibrational resonance of a selected element.

The invention belongs to the technical field of manufacturing nano mesoporous material, in particular to a novel synthesis technology of Beta to diketone functionalized rare earth mesoporous hybrization and luminescent material. The invention first adopts a method of organic modification and synthesis to covalently graft an organic ligand molecule with Beta to diketone structure in a main body framework of an inorganic mesoporous, then rare earth europium or terbium ion is introduced to synthesize the high ordered mesoporous hybrization and luminescent material with steady chemistry and thermodynamic performance. The method and experimenting condition of the invention are moderate, the whole manufacturing system is easy to be established, the operation is easy, and the condition is easy for controlling, so the obtained products has steady quality. .

It is intended to provide a novel contrast agent for photoacoustic imaging that is highly capable of binding to a target molecule and generates high photoacoustic signals. The present invention provides a contrast agent for photoacoustic imaging represented by Formula 1:

MNP-((L)_l-(P)_m)_n  (Formula 1)

(wherein MNP represents a particle containing an iron oxide particle; L represents a linker molecule; P represents a ligand molecule; l represents 0 or 1; and m and n represent an integer of 1 or larger), the contrast agent for photoacoustic imaging including: a particle containing an iron oxide particle that absorbs light in a near-infrared region; and at least one or more ligand molecule(s) immobilized on the particle containing an iron oxide particle, wherein the immobilization density of the ligand molecule is equal to or higher than the cell surface density of a target molecule.

The invention provides a symmetrical difunctional coupling agent compound (I), i.e. N-fluorenylmethyloxycarbonyl-L-beta-glutamate-di-N-succinimide (Fmoc-beta-Glu(OSu)-OSu), and a production method thereof. Based on the compound, a series of novel coupling compounds which respectively contain a symmetrical difunctional coupling base, i.e. beta-Glu (beta-glutamate) are prepared through being coupled to ligand molecules; the structural formula of the series of coupling compounds is shown as (II), wherein M1 is a -NH2-contained ligand of a target molecule T1, M2 is a -NH2-contained ligand of a target molecule T2, L is a linking group, and S is a report signal group. The compound (1) provided by the invention has been used in coupling a targeted integrin alphavbeta3 receptor ligands, gastrin-releasing peptide receptor ligands, telomerase inhibitor pharmacophores and epidermal growth factor receptor ligands, and thus, a plurality of coupled bi-ligand molecular imaging agents and coupled tri-ligand molecular imaging agents are synthesized. The invention further relates to the use of the compound (I) and the series of novel coupling compounds (II) in the preparation of imaging agent drugs.

A method is provided for detecting target molecules (60) using a surface-molecularly imprinted sensor (52) and more particularly for detecting hydroxyl containing molecules, heavy metal ions, or thiol- or dialkylamine containing molecules by integrating molecular recognition and sensor transduction. The method includes soaking a support surface (14) in a solution containing a ligand molecule with headgroups that could adsorb on the surface of a substrate, or monomer to create a monolayer, a monolayer of polymerized organosiloxane groups (16), or a conducting film on the support surface (14). Template molecules (18) are then added to the imprinting solution so as to be positioned within the sparsely formed film, wherein the template molecules (18) comprise hydroxyl containing molecules, heavy metal ions, or thiol and dialkylamine containing molecules which prevents the interaction between monomer/ligand molecules and template molecules (18). In this case, there is a competitive reaction between the hydroxyl group on the surface of substrate and hydroxyl in ethanol molecule with monomer, e.g. OTS. The template molecules (18) are removed to define cavities in the imprinted film (16).

Disclosed are certain peptide linkers for conjugating drugs to ligands, and the resulting drug-linker-ligand molecules and compositions thereof. The conjugated molecules useful for the targeted delivery of drugs to the desired cells, and allow for the intracellular release of the drug in cases where the targeted antigen is internalized via the trans Golgi network and not the lysosomal pathway.

The invention discloses a method for predicting the binding free energy of protein and ligand based on a progressive neural network, and belongs to the technical field of computer-aided drug design. The method comprises the steps: obtaining a pdb file from a PDBbind database, establishing local database, acquiring an amino acid molecule within 4.5 angstroms in the protein binding pocket by takingthe ligand molecule as a center, performing extended connectivity fingerprint calculation, carrying out SPLIF fingerprint calculation, searching for the number of salt bridges and hydrogen bonds between protein and ligand molecules, converting the structural information of the protein and the ligand into a one-dimensional tensor, and establishing a training set, a verification set and a test set;training the progressive neural network by using the training set; optimizing and searching hyper-parameters for prediction; through comparison with a molecular docking result, obtaining a higher Pearson correlation coefficient. According to the invention, the technical problem of how to convert a three-dimensional structure of protein and ligand molecules into tensors which are easy to calculateby a computer and input the tensors into the progressive neural network for training and optimization is solved, and the calculation rate and the prediction accuracy are greatly improved.

The invention relates to a chiral diphosphine ligand and a chiral catalyst, and a preparation and application method thereof. The preparation method of a BINAP (2,2'-bis(diphenylphosphino)-1,1'-binaphthyl) chiral diphosphine ligand based on novel imidazole and imidazole cation modification. Imidazole and imidazole cation are introduced to the 5,5'- position of the BINAP molecule framework to synthesize the chiral diphosphine ligand. The assembly of the imidazole and imidazole cation into the chiral diphosphine ligand molecule can effectively enhance the stability of the chiral catalyst in an ionic liquid and the affinity with the imidazole ionic liquid, and avoids loss of the catalyst in the cyclic process. The imidazole-modified chiral diphosphine ligand has the following chemical structural formula, wherein the spatial configuration of the imidazole-modified chiral diphosphine ligand is S type or R type.

The invention provides a preparation method of a Bi2Te3 dimensional nano tablet. The preparation method comprises a step of subjecting a Bi2Te3 nano material, water, and functional ligand molecules to ultrasonic exfoliation so as to obtain the Bi2Te3 dimensional nano tablet; wherein the functional ligand molecule is polyvinylpyrrolidone, cytochrome C, or bovine serum albumin. In the preparation method, the functional ligand molecule is taken as the stripping agent to carry out ultrasonic exfoliation on a Bi2Te3 nano material so as to obtain a functional ligand molecule-Bi2Te3 dimensional nano tablet with high water solubility; and the dimensional nano tablet has the advantages of uniform particle size, controllable morphology, high water solubility, good biocompatibility, and high photo-thermal conversion efficiency, is a good photo-thermal agent, and can be applied to tumor photo-thermal therapy. The provided preparation method can synthesize massive water-soluble Bi2Te3 with a photo-thermal property, the preparation process is simple, the cost is low, and the preparation method is suitable for industrial production.

The invention discloses a method for detecting fungaltoxin through multiple signals. The method comprises the following steps: 1) combining fungaltoxin with aptamer: adding a to-be-detected sample after the reaction of the fungaltoxin aptamer and complementary sequence thereof, thereby acquiring a mixed solution A; 2) amplifying a digestion auxiliary signal: reacting the mixed solution A with restriction enzyme, thereby acquiring a mixed solution B; 3) preparing a guanine tetramer structure: reacting the mixed solution B with tail-end deoxynucleotide transferase and deoxyribonucleoside triphosphate and reacting with ligand molecules, thereby acquiring a mixed solution C; and 4) detecting and analyzing: performing catalytic oxidation reaction on the mixed solution C and different substrates, generating ultraviolet, fluorescent and chemiluminescent signals and calculating the content of fungaltoxin in the to-be-detected sample according to the relation of the response strength of all the signals and the fungaltoxin concentration. The method disclosed by the invention has the characteristics of quick and simple operation and treatment, short detection time, marker-free effect, low cost, high precision, high sensitivity, and the like.

The invention relates to a method to directly add trace sample and instantly react on the biomolecule chip and device. The method includes: under the action of external pressure, the surfaces of microarray and smooth chips close contact to form a sealed cavity. The bio-ligand molecule which needs fixing enters the cavity through the micro-flow channel and reacts with the chip surfaces, and after reacting, the ligand molecule is discharged through the micro-flow channel. The device includes: elastic material slice carved with grooves, microarray arranged of grooves and micro-flow channel matching the microarray; and elastic material membrane covered on the micro-flow channel, where there is a liquid entrance made on the base slice of microarray or micro-flow channel. After fixed and reacting, the chip surfaces are washed by buffer liquor, to make fixation and reaction of the biomolecule on the surface uniform and consistent, effectively heightening the detecting quality.

The invention discloses an NMR nuclear magnetic resonance method for high flux medicine screening. The method includes the following steps: a, preparing mixed water solution containing target biomacromolecules and to-be-screened medicine, and adding the target biomacromolecules and to-be-screened molecules in the water solution and uniformly mixing the biomacromolecules and the to-be-screened molecules as well as the water solution; b, filling the mixed solution in an NMR sample tube, placing the sample tube in a spectrometer, and selectively detecting signals of ligand molecules combined with the target protein, wherein the selective detection includes the steps that firstly, building the selective disturbance for water serving as the resolvent based on the radiation damping effect, secondly, performing the operation based on the effects such as exchange and polarization transfer, and thirdly, exciting the built signals, and selectively detecting the protein and the ligand molecules of the protein, wherein the operation includes two pulse modular units, namely, a signal building module and a detection module; and c, completing the molecule confirmation. The method is simple and convenient to operate and feasible, has high accuracy and sensitivity in experiment detection, and effectively improves the accuracy and efficiency in medicine screening.

This invention relates to a photosensitive shining Europium complex and its photosensitive ligand molecule, also relates to the synthetic method. The Europium complex structure is showd as VI: the R1, R2 is alkyl with 1 to 4 carbon atoms, R3, R4, R5, and R6 are same and are methyl or H, the R8 is methyl or H. The organic photosensitive ligand molecule structural formula is showd as I: the R1, R2 is alkyl with 1 to 4 carbon atoms, R3, R4, R5, and R6 are same and are methyl or H, the R8 is methyl or H. The photoallergy molecule possess fine long wave photoactivate Europium ion luminance. The new style Europium complex possess width activating window, at the same time possess large two-photon absorption acting section and / or high rare earth ion fluorescence quanta yield, is useful for preparing excellent bioluminescence probe stuff, possess comprehensive application prospects.

An objective of the invention is to provide a drug delivery vehicle capable of allowing a vaccine or adjuvant to reach a target cell or tissue efficiently while being capable of improving the immunogenicity of the vaccine or capable of enhancing the immunostimulating effect of the adjuvant as well as a vaccine or adjuvant utilizing the same. Said drug delivery vehicle contains a multimeric protein having a coiled coil structure and a ligand molecule to a receptor of an immune cell.

The invention discloses a preparation method of a mercury ion colorimetric sensing cellulose material, which comprises the following steps: taking a natural cellulose fiber material as a matrix, taking tetrabutyl titanate as a precursor and depositing a titanium dioxide film on the surface of a filter paper fiber by using a surface sol-gel method; then self-assembling and introducing a ruthenium dye N719 ligand molecule monolayer sensitive to mercury ions to obtain the mercury ion colorimetric sensing cellulose material; and detecting the mercury ions in the solution by simply infiltrating the material prepared by the invention in water solution of the mercury ions for a certain time and observing the change of the solution. In addition, the material prepared by the invention can also realize the recycling after treated by potassium iodide solution. The mercury ion colorimetric sensing cellulose material has excellent sensitivity and selectivity on the mercury ions and has the advantages of abundant sources of raw materials, low price, simple preparation method and capability of realizing simple, quick and economic detection on the mercury ions.

The invention provides a binding layer comprising a polysaccharide substituted by carboxylic groups or derivatives thereof exhibiting high performance in the binding of ligand molecules and in the interaction thereof with analyte molecules. A method for the preparation of the binding layer and for the assaying of various analyte molecules is also provided.

The invention discloses a KIR and ligand genetic typing experimental method. By the technology, the time and labor are saved, and meanwhile, DNA sample capacity is further saved. A multi-PCR technology is adopted, meanwhile, 36 pairs of primers are further combined according to the sizes of PCR products, and finally, amplified reaction is finished in 12 reaction holes. The KIR and ligand genetic typing experimental method is conveniently applied to amplification of 96 pore plates, conventional Taq enzyme is used, typing of all KIR genes and ligands thereof can be finished at a time under the same reaction conditions, and the practicality of the KIR and ligand genetic typing experimental method is greatly improved. Two pairs of primers are used for a KIR gene, the accuracy is improved, anda false negative result is avoided. The KIR gene can be combined to MHC-I type ligand molecules on the surfaces of targeting cells, inhibiting or activating signals are transmitted to regulate the activity of NK cells and T cells, and the KIR and ligand genetic typing experimental method plays an important regulation role in hematopoietic stem cell transplantation, feto-matemal tolerance, anti-infectious immunity, tumor immunity and autoimmune diseases. Therefore, KIR genetic typing facilitates understanding of influences of KIR to tumor immunity, hematopoietic stem cell transplantation and autoimmune diseases.

Methods for searching stable docking models of biopolymer-ligand molecule complex, which comprise the steps of: (1) searching possible hydrogen-bond schemes between a biopolymer and a ligand molecule by preparing possible combination sets of hydrogen-bonding heteroatoms in the ligand molecule with dummy atoms located at the positions of heteroatoms that can be hydrogen-bond partners to hydrogen-bonding functional groups in the biopolymer; (2) estimating the possible the hydrogen-bond schemes between the biopolymer and ligand molecule and the possible conformations of a hydrogen-bonding part of the ligand molecule at the same time by comparing the distances between the dummy atoms with the distances between the hydrogen-bonding heteroatoms; and (3) obtaining the possible docking models of the biopolymer-ligand molecule complex by changing the coordinates of all atoms of the ligand molecule into the coordinate system of the biopolymer, according to the correspondences between the hydrogen-bonding heteroatoms in the ligand molecule and the dummy atoms in combination sets for each of the hydrogen-bond schemes and conformations obtained in the second step are provided. According to the methods of the present invention, the structures of stable biopolymer-ligand molecule complex can be searched efficiently and precisely in a short time.

The invention discloses a method for preparing monoatomic catalysts. The method comprises the following steps: step 1, preparation of a transition metal precursor: separately weighing and adding organic amine and substituted benzaldehyde into an acetonitrile solution to obtain an intermediate product, carrying out reduction, extraction and drying to obtain an organic ligand molecule product, separately dissolving organic ligand molecules and a transition metal salt in an acetonitrile solution, continuing mixing under stirring complete dissolving, and successively carrying out filtering, washing and drying so as to obtain a transition metal precursor product; step 2, mechanical ball milling: dispersing the transition metal precursor in carbon black with a high specific surface area by usinga high-energy mechanical ball milling process to obtain a monoatomic catalyst precursor; and step 3, carrying out high-temperature calcinations so as to obtain corresponding monoatomic catalysts. Theinvention realizes the preparation of transition metal monoatomic catalyst materials, has good expandability and reproducibility, and solves the problems of low metal content, few varieties and complicated preparation process in the prior art.