8 results about "False Negative Result" patented technology

The invention discloses a biosensor based on 3D DNA Walker and UEDC and application, and relates to the technical field of biosensors, the biosensor comprises WB-LS (at) SMBs, Nb.BbvCI, a 10 * CutSmart buffer solution, an auxiliary chain A solution and ultrapure water, the sequence of the auxiliary chain A is SEQ ID NO.1 in a sequence table; according to the biosensor based on the 3D DNA Walker and the UEDC and the application of the biosensor, non-sugar substances are detected by utilizing a common glucometer through the biosensor, high-sensitivity detection can be performed on HIV-DNA, the detection range is wide, accurate detection can be performed from 5 pM to 20 nM, and the detection line is as low as 0.3 pM; and the biosensor has good base mismatch recognition capability, so that false positive or false negative results caused by base mismatch are effectively avoided, and the detection accuracy and reliability are improved.

The present application relates to the technical field of gene detection, in particular to a primer probe combination for detecting Alzheimer's disease, a product and application thereof. The primer probe combination and kit provided by the present application can simultaneously detect Alzheimer's disease gene mutation and methylation, can effectively distinguish Alzheimer's disease patients from non-Alzheimer's disease subjects, realize low-invasive, low-cost, high-sensitivity, high-specificity detection of Alzheimer's disease, the total sensitivity of the blood sample for detecting Alzheimer's disease can reach 88.89%, the specificity of the blood sample for detecting non-Alzheimer's disease can reach 95.74%, the detection probability of false negative results can be reduced, and the detection accuracy is high.

This invention relates to the field of gene detection technology, and in particular to a primer-probe combination, product, and application for detecting Alzheimer's disease. The primer-probe combination and kit provided by this invention can simultaneously detect Alzheimer's gene mutations and methylation, effectively distinguishing between Alzheimer's patients and non-Alzheimer's subjects. It achieves low-invasiveness, low cost, high sensitivity, and high specificity in Alzheimer's disease detection. The overall sensitivity for detecting Alzheimer's blood samples reaches 88.89%, and the specificity for detecting non-Alzheimer's blood samples reaches 95.74%, reducing the probability of false negatives and demonstrating high accuracy.

The utility model belongs to the technical field of biomedical detection, and discloses a rapid detection kit for gastric cancer nerve invasion, which comprises a cover plate hinged to the top of a kit body, and a sampling tube placing rack is embedded in the top of the kit body. Three first placing cavities, three second placing cavities and a third placing cavity are formed in the top of the kit body, a plurality of placing grooves are formed in the top of the sampling tube placing frame, collecting tubes are arranged in the placing grooves, each collecting tube comprises a first dropper fixedly connected to the center of the bottom of a tube body, and a second dropper fixedly connected to the center of the bottom of the tube body. The top of the tube body is in threaded connection with a tube cover, and a first cavity is formed in the first dropper. Complex equipment and operation steps are not needed, clinical application is facilitated, the time from sample collection to result output is greatly shortened, markers with extremely low concentration can be detected, the detection rate of early gastric cancer nerve invasion is increased, false positive and false negative results are reduced, and the detection accuracy is improved.

The invention discloses a protein and nucleic acid bimodal detection method based on a digital microfluidic platform, which can quickly detect HBsAg and complete HBV genetic typing under the condition that only a trace amount of serum sample is needed. In the detection process, the probe AuNPs (at) Ab specifically recognizes an antigen, nucleic acid detection is combined with multiple signal enhancement systems such as recombinase polymerase amplification, a T7 transcription technology and a clustering rule interval short text repetition technology to generate a superbright fluorescence signal, so that a false negative result is effectively avoided. Compared with the traditional diagnosis process, the DMF platform shortens the detection time from 3-4 hours to 40 minutes. According to the innovative diagnosis strategy, the efficiency and reliability of infectious disease detection are remarkably improved, and a new thought and a technical platform are provided for early screening, rapid diagnosis and public health management of infectious diseases.

The invention belongs to the technical field of virus detection, and particularly relates to a quick constant-temperature detection kit for Kucurajie virus and application of the quick constant-temperature detection kit. The primer, the probe and the reaction system designed by the kit have extremely high sensitivity, the lower detection limit is as low as 5 copies / mL, the accuracy rate reaches 100%, the specificity is high, and cross amplification with other 80 pathogens is avoided. A recombinase polymerase isothermal amplification technology is adopted, large PCR equipment does not need to be used, the detection time is only 25 minutes, and nearly 2 hours are saved compared with a traditional fluorescent probe PCR method. In addition, the kit is provided with an M13 bacteriophage probe for internal positive quality control, false negative results are effectively prevented, and meanwhile detection pollution is avoided by adding UNG enzyme. The kit is suitable for laboratory detection, field detection and bedside detection, and is especially suitable for rapid detection of the Kucuragarie virus at quarantine ports, airports and epidemic outbreak sites.

This invention relates to the field of detection kit technology, specifically to an African swine fever virus pathogen detection kit and detection method. This invention provides an African swine fever virus detection kit and detection method integrating specific primers and probes, a multiplex quality control system, and standardized operating procedures. By optimizing the nucleic acid extraction process, establishing a dual-channel fluorescent PCR detection system, and defining clear judgment criteria, it achieves high sensitivity and high specificity detection of African swine fever virus nucleic acid and full-process quality monitoring. It can not only accurately identify virus-positive samples but also effectively exclude false negative results through an internal standard system. This solves the key problems of insufficient specificity and low reliability of traditional detection methods, providing a stable and reliable complete technical solution for the clinical diagnosis and prevention and control of African swine fever.

A sanitation management system maintains records of exemplary locations on an item to be sanitized and indicates those locations to a person or system charged with applying a pathogen surrogate prior to a sanitation process. The sanitation management system maintains records of where the pathogen surrogate was applied prior to the sanitation process. Following the sanitation process, the sanitation management system indicates to an inspector, or an inspection system, locations of where the pathogen surrogate was applied for the purpose of facilitating testing of the sanitation process by checking for the presence of the pathogen surrogate at some or all of those locations. This can ensure that the inspection process is relevant to the sanitation process and less likely to generate false negatives where the inspection finds a lack of the pathogen surrogate not due to cleaning, but due to lack of application of the pathogen surrogate.