The invention discloses a real-time fluorescent quantitative PCR detection method of human breast cancer genotypes. The method comprises the following steps: ERa (SEQID9), PR(SEQID10) and HER-2(SEQID11) of the human breast cancer gene are taken as augmented target gene sequences, 18SrRNA(SEQID12) is taken as an internal control gene, specific primers are designed corresponding to the ERa gene, the PR gene, the HER-2 gene and the 18SrRNA gene respectively, CT values of a sample to be detected and a control sample are detected through adopting the real-time fluorescent quantitative PCR method, and the target gene expression level is calculated; the target gene expression level is analyzed through the delta delta CT method, the target gene expression level equals to 2<delta delta CT>, wherein delta delta CT equals to (CT, target minus CT, 18S)(detected) minus (CT, target minus CT, 18S)(control); when the target gene expression level is less than or equal to 1, the ERa gene, the PR gene and the HER-2 gene are subject to negative typing; and when the target gene expression level is more than 1, the ERa gene, the PR gene and the HER-2 gene are subject to positive typing. The invention has the advantages of simplicity and convenience in operation, high detection sensitivity, short detection time, good specificity and repetitiveness, accurate and reliable results and qualitative and quantitative detection, can be used for genotype identification of common populations or patients with breast cancer, provides an important theoretical index on personalized for the patients with breast cancer to personally take medicine, has significance for prognosis of the patients with breast cancer, and has stronger popularization and practicability.