72 results about "Transcription initiation" patented technology

Compositions and methods are provided for the treatment and diagnosis of diseases amenable to treatment through modulation of the synthesis or metabolism of intercellular adhesion molecules. In accordance with preferred embodiments, oligonucleotides are provided which are specifically hybridizable with nucleic acids encoding intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial leukocyte adhesion molecule-1. The oligonucleotide comprises nucleotide units sufficient in identity and number to effect said specific hybridization. In other preferred embodiments, the oligonucleotides are specifically hybridizable with a transcription initiation site, a translation initiation site, 5'-untranslated sequences, 3'-untranslated sequences, and intervening sequences. Methods of treating animals suffering from disease amenable to therapeutic intervention by modulating cell adhesion proteins with an oligonucleotide specifically hybridizable with RNA or DNA corresponding to one of the foregoing proteins are disclosed. Methods for treatment of diseases responding to modulation cell adhesion molecules are disclosed.

A process for producing transgenic plants or plant cells capable of expressing a coding sequence of interest under transcriptional and translational control of host nuclear transcriptional and translational elements is described by introducing into the nuclear genome of host plants or plant cells a vector comprising said coding sequence of interest which is devoid of (a) an upstream element of initiation of transcription functional in the host plants or plant cells and operably linked to said coding sequence of interest and required for its transcription; (b) an upstream element of initiation of translation functional in the host plants or plant cells and operably linked to said coding sequence of interest; and subsequently selecting plant cells or plants expressing said coding sequence of interest.

The invention relates to a high-efficiency artificial activating transcription factor dCas9-TV, and a coding gene and applications thereof. According to a construction method, the carboxyl terminal ofnuclease inactivated Cas9 protein (dCas9) is connected with a plurality of copies of VP64 and TAL transcription-activating domains so as to obtain a series of novel artificial activating transcription factors, and obtain dCas9-TV with the best transcriptional activation activity via screening. When only one guide RNA (gRNA) is adopted for targeting a specific gene promoter, dCas9-TV is capable ofrealizing high efficiency activating of transcription of endogenous genes of Arabidopis thaliana and paddy rice; when a plurality of gRNA are adopted for targeting a plurality of target genes, dCas0-TV is capable of realizing transcription activation of a plurality of genes. In addition, it is confirmed that dCas9-TV possesses the same high efficiency targeting transcription activation activity in human cells. An in vitro assembled dCas9-TV/gRNA ribonucleoprotein compound can be adopted for transcription activation of Arabidopis thaliana and paddy rice endogenous genes. The high-efficiency artificial activating transcription factor dCas9-TV can be adopted in the fields such as genome genetic screening, metabolite biosynthesis pathway reconstruction, and crop improvement.

A novel mini-retrotransposon vector system is provided for integrating foreign DNA into plants. The invention includes a novel vector comprising a truncated and modified retroelement which includes the 5′ and 3′ LTR regions that provide transcription initiation and termination sites as well as the cis acting sequences required for reverse transcription. Novel vectors, plant cells, and methods of using the same are disclosed.

Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

The invention discloses a plant gene promoter and the application. It is has one nucleotide sequence from: the DNA sequence in SEQ ID No: 1; nucleotide sequence of DNA sequence intercross limited in SEQ ID No: 1; the nucleotide sequence has transcription initiation function and has 90% homology of the nucleotide sequence limited by SEQ ID No: 1. The tobacco transgene examination proves theat pGhGlcat1 could endue report gene having high level expression in epidermal hair, top meristem region and androecium and gynoecia of plant flower apparatus. It could be used to improve the plant quality and the gene engineer of fastness.

The invention discloses a populus deltoidesx populus nigra resistance-related gene PdMYB2 which plays an important role in the plant salt resistance and drought resistance process. The provided bZIP gene is named as PdMYB2, and the gene has a base sequence shown in SEQ ID NO:3 of a sequence table. When the resistance-related gene PdMYB2 is constructed to an expression vector pCAMBIA1304, a promoter is added in front of a transcription initiation nucleotide, a selective marker GFP is added to authenticate and screen transgenosis vegetable cells or plants, and the expression vector carrying the resistance-related gene PdMYB2 can convert plant hosts through multiple methods and is used for cultivating salt-resistant and drought-resistant plant varieties. The gene has wide application prospects in cultivation of salt-resistance and drought-resistant plants.

Peptide nucleic acids and analogues of peptide nucleic acids are used to form duplex, triplex, and other structures with nucleic acids and to modify nucleic acids. The peptide nucleic acids and analogues thereof also are used to modulate protein activity through, for example, transcription arrest, transcription initiation, and site specific cleavage of nucleic acids.

A novel beta -galactosidase gene family and DNA sequences derived from the cloning of cDNAs encoding products of these genes are provided, as exemplified by a beta -galactosidase II protein which is encoded by a cDNA clone, pZBG2-1-4. A method for modifying cell wall metabolism which involves modifying the activity of at least one beta -galactosidase, and thus modifying the quality of the fruit is also provided. Also provided by the present invention is a DNA construct including some or all of a beta -galactosidase DNA sequence under control of a transcriptional initiation region operative in plants, so that the construct can generate RNA and, optionally, beta -galactosidase polypeptide in plant cells. The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells and for using them for production of beta -galactosidase polypeptides or peptides by recombinant techniques. The present invention also provides plant cells containing DNA constructs of the present invention; plants derived therefrom having modified beta -galactosidase gene expression; and seeds produced from such plants.

The present invention provides virus vectors of the family Paramyxoviridae in which the transcription start (S) sequence has been modified so as to modify the expression of genes located downstream thereof, a method for producing the vectors, and uses thereof. By measuring the transcription initiation efficiency of the S sequence of each gene carried by Sendai viruses (SeV), it was clarified that the S sequence of F gene has a significantly lower ability to promote transcription than the other three S sequences. When the S sequence of the F gene of wild type Sendai virus was substituted by the S sequence of the P/M/HN gene-type showing a high transcription initiation efficiency, the F gene of the resultant Sendai virus mutant and genes located downstream thereof show elevated expression levels. It was also revealed that this mutant proliferates more quickly than the wild type. The vectors of this invention are useful in elevating the expression of foreign genes and producing pharmaceutical compositions and vaccines. Furthermore, by lowering virus gene expression from virus vectors, it is possible to suppress transcription and/or replication and reduce cytotoxicity of the vector genome.

The invention relates to an RASSF1A gene methylation state detection kit. The kit comprises a stem-loop primer, and the stem-loop primer is used for performing specific amplification detection on CpG island-rich sequences in the upstream -1bp to -187bp of the transcriptional initiation region of an RASSF1A gene. The stem-loop primer designed by the invention can significantly improve the detection efficiency of methylated DNA in DNA and prevent non-specific amplification of an unmethylated DNA template; and the kit improves the sensitivity of detection while the specificity of tumor detection is improved.

A novel β-galactosidase gene family and DNA sequences derived from the cloning of cDNAs encoding products of these genes are provided, as exemplified by a β-galactosidase II protein which is encoded by a cDNA clone, pZBG2-1-4. A method for modifying cell wall metabolism which involves modifying the activity of at least one β-galactosidase, and thus modifying the quality of the fruit is also provided. Also provided by the present invention is a DNA construct including some or all of a β-galactosidase DNA sequence under control of a transcriptional initiation region operative in plants, so that the construct can generate RNA and, optionally, β-galactosidase polypeptide in plant cells. The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells and for using them for production of β-galactosidase polypeptides or peptides by recombinant techniques. The present invention also provides plant cells containing DNA constructs of the present invention; plants derived therefrom having modified β-galactosidase gene expression; and seeds produced from such plants.

Provided herein are short exogenous fungi transcription promoter nucleic acid sequences and methods of using the exogenous fungi transcription promoter nucleic acid sequences to modulate transcription initiation or rate of transcription.

A DNA comprising a 609 bp base sequence from -559 to +50 when the first base sequence of exon 1 of the midkine gene, a human retinoic acid-responsive growth/differentiation factor was set as +1, or a DNA comprising a 251 bp base sequence from -213 to +38 when the transcription initiation point of the c-erbB-2 gene belonging to the EGF receptor family and having a tyrosine kinase activity was set as +1 has a tumor-specific transcription activity, and the promoter activity thereof is high, and therefore is very important as a tumor-specific promoter for use in the suicide gene therapy that combines the use of a gene for a drug metabolizing enzyme and a prodrug for cancer therapy, the gene therapy of cancer using an expression vector that contains a gene encoding a cytokine, and the gene therapy of cancer using an oncolytic virus that exhibits cytotoxic effects only on tumor cells, etc.

The invention discloses a newly found DNA sequence of the promoter of the chalcone synthase (chs) gene and the manufacturing method and use for the same. The promoter is used for PCR with a joint from sorbonne, and clones 899bp of the upstream regulation sequence of chs gene by combining the characteristics of the nested PCR, TD PCR, hot-started PCR and two-step PCR. The analysis in the promoter database Plant CARE shows that, the sequence has a basically conservative region TACPyAT which expresses specifically in flowers, and has the main cis-acting elements with which the primary promote is provided such as a transcription initiation related TATA box, a transcription auxiliary CAAT box, a G box and CCAAT etc. The promoter can regulate the specific expression of the target gene in the plant, and can provide an effective promoter element for the constructing the plant expression vector, increase the expression of foreign gene, and minimum the biological energy consumption.

The invention relates to a kit for detecting the methylation status of Septin 9 genes. The kit includes a hairpin-shaped primer, wherein the primer is applied special amplification detection of a CpGisland-rich sequence in an exon 1 of the second transcript NM_001113493.2 of the Septin 9 genes. Through screening and comparison, it is shown that the segment, of a length of 158 bp, in the backwardposition of a transcription initiation point of the second transcript NM_001113493.2 of the Septin 9 genes is a region with the most significant methylation change in the tumor tissue. Through the designed hairpin-shaped primer, the detection efficiency of methylated DNA in DNA can be improved significantly, and non-specific amplification of unmethylated DNA templates is prevented.

Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism. The recombinant microorganism is produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the microorganism strain is prepared by: introducing a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism into the upstream of a Bacillus subtilis prsA gene or a gene corresponding thereto in the genome of a parental microorganism, or introducing a gene fragment prepared by ligating a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism to the upstream of the Bacillus subtilis prsA gene or a gene corresponding thereto into the genome of a parental microorganism; and deleting or inactivating one or more genes selected from an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, a dltE gene, and a gene (genes) corresponding thereto.