PCR and short segment DNA sequencing combined diagnosis method

Abstract

The diagnosis method combining PCR and short segment DNA sequencing relates to a biological detection method, specially it is applicable to the research fields of antenated diagnosis of genetic disease, detection of pathogenic pathogen, detection and diagnosis of cancer gene, genetic fingerprinting, individual identification and offspring-parent relationship determination, animal and plant quaretine and biological emdicine, etc. Said invention uses two ends of micro target gene or two-side known nucleotide sequences to design a pair of primers, and uses DNA to be identified as template to make PCR amplification.

Description

technical field [0001] The diagnostic method combining polymerase chain reaction and short-segment DNA sequencing of the present invention relates to a biological detection method, and is especially suitable for prenatal diagnosis of genetic diseases, detection of pathogenic pathogens, detection and diagnosis of cancer genes, DNA fingerprints, Individual identification, parent-child relationship identification, forensic evidence, animal and plant quarantine, high-tech biomedicine and other fields. technical background [0002] In recent years, there have been revolutionary breakthroughs in gene analysis and genetic engineering technology, which is mainly due to the development and application of polymerase chain reaction (polymerase chain reaction, PCR). The application of PCR technology can make a specific gene or DNA fragment amplified hundreds of thousands to one million times in vitro in just 2-3 hours. The amplified fragments can be directly observed by electrophoresis...

AI Technical Summary

Problems solved by technology

However, false positive errors occur. In addition to contamination being a major factor, the defect of PCR technology itself is also an important reason.

The annealing temperature design is difficult to be accurate, and the mismatch of primers and a certain degree of single nucleotide incorporation can lead to false positive results.

Moreover, it is very time-consuming and labor-intensive to find the cause and correct it

Therefore, a successful PCR detection method needs to go through a long period of exploration conditions and actual verification before it can be finally applied to clinical practice, and in the actual operation process, the requirements for operating specifications are relatively high, which also limits its application to a certain extent.