The invention provides a
protein extraction method and an application, and particularly relates to a method for extracting
protein from paraffin-embedded tissues and an application. According to the method, toxic
xylene is replaced by a non-toxic TO transparent agent, so that the method is safe and environment-friendly; the
protein is subjected to extraction treatment by using a high-temperature boiling method, so that low-abundance
protein degradation and loss caused by tissue freeze-thaw
cracking or ultrasonic crushing of a conventional operation is avoided; an
antigen repairing step of an
antigen repairing liquid adopting
microwave heating is replaced by using
metal bath heating; and by virtue of match of a
nest screen cloth
cell, the operation is simple and feasible, and the integrityof a tissue slice can be better guaranteed. The method not only improves the extraction amount of the
total protein of the paraffin tissues, but also increases the detection sensitivity to the low-abundance protein; and the overall operation process is simple, feasible and time-saving, and is safe, non-toxic and low in cost.