4 results about "Synthetic DNA" patented technology

The disclosure provides for methods of constructing synthetic chromosomes including the steps of providing host cells with an endogenous chromosome, transforming a cloning vector and a cloning cassette into the host cells, excising target genomic nucleic acids from the endogenous chromosome, recombining the excised target genomic nucleic acids with the cloning cassette via homologous recombination to form heterologous vectors comprising cloned sequences, extracting the heterologous vectors containing the cloned sequences from the host cells, digesting the heterologous vectors with a restriction endonuclease to release the cloned sequences from the heterologous vectors to provide released cloned sequences, and introducing the released cloned sequences, a centromere cassette, and a yeast artificial chromosomes or bacterial artificial chromosomes into a second host cell, such that the released cloned sequences, the centromere cassette, and the YAC or BAC recombine with one another via homologous recombination to produce the synthetic chromosome.

The application discloses an aquifer delineation method and device based on synthetic DNA-thermal double tracing, and relates to the technical field of hydrogeophysical tracer tomography imaging, and the method comprises the following steps: obtaining DNA tracer travel time data and thermal tracer travel time data collected at an observation well after simultaneously injecting different types of synthetic DNA tracers and thermal tracers at multiple depths of an injection well of a target aquifer system; according to the DNA tracer travel time data and the thermal tracer travel time data, applying a SIRT inversion method based on a double-tracer mechanism to inversely calculate the permeability coefficient of the target aquifer, and further obtaining a water diffusion coefficient, a water storage rate and thermal characteristic parameters. The permeability coefficient of the target aquifer is inversely calculated through the double-tracer mechanism, and the aquifer heterogeneity can be accurately delineated.

A synthetic DNA molecule comprising one segment encoding a tumor neoantigen or an epitope from an infectious agent under the control of a promoter for the transcription into a corresponding RNA molecule and a segment for the translation of the translated RNA molecule into a peptide.

Disclosed herein are mutants of bacteriophage Phi29 DNA polymerase with improved primer recognition, compared to the wild-type enzyme. Certain mutants comprise one or both of the mutations K64R or M97K. The provided mutants are capable of using more efficiently shorter and longer random synthetic DNA primers than wild-type Phi29 DNA polymerase, generating more amplification product in Multiple Displacement Amplification (MDA) reactions. The inventive mutants amplify human genomic DNA with less bias and better coverage in comparison to reactions carried out with wild-type Phi29 DNA polymerase.