28 results about "Synthetic DNA" patented technology

The invention discloses a photonic crystal fluorescence enhanced printing synthesis DNA chip cleaning effect verification method, which comprises: S1, preparing a photonic crystal microchip, and forming a hydrophobic substrate and a hydrophilic detection area; s2, building a chip test platform, collecting a reaction cleaning liquid to-be-tested sample within a specific time, and dropwise adding the to-be-tested sample to the surface of the photonic crystal microchip; s3, heating the photonic crystal microchip, and enriching the to-be-detected substance in the hydrophilic detection area after the sample solution is dried; s4, detecting the fluorescence spectrum signal emitted by the to-be-detected object through fluorescence spectrometry so as to realize detection of the to-be-detected object; and S5, judging the cleaning effect of the chip according to the change of the fluorescence intensity. According to the verification method, the chip cleaning effect can be judged according to the fluorescence intensity change, the method is also suitable for trace detection under the extremely low concentration condition, the sensitivity higher than that of a common substrate such as glass is achieved, the detection limit is reduced, and more accurate analysis under the low concentration condition is achieved.

The present invention relates to a method of selecting a polypeptide for display on a genetic display system according to activity, comprising the steps of: displaying the polypeptide on the genetic display system, screening a system for binding to a target, and selecting a member binding to the target; cloning a nucleic acid encoding the polypeptide from a member of the display system, and expressing the nucleic acid in an expression system to produce the polypeptide, or sequencing the polypeptide from a member of the display system, and cloning and expressing the polypeptide or a library of polypeptides using synthesis-based DNA; and determining the activity of the polypeptide in an assay.

A synthetic DNA molecule comprising one segment encoding a tumor neoantigen or an epitope from an infectious agent under the control of a promoter for the transcription into a corresponding RNA molecule and a segment for the translation of the said translated RNA molecule into a peptide.

The invention belongs to the technical field of biomedical materials, and particularly relates to mineralized DNA tetrahedral framework nucleic acid hydrogel as well as a preparation method and application thereof. The synthetic DNA tetrahedral framework nucleic acid hydrogel is subjected to mineralization treatment, so that calcium phosphate deposition is formed on the surface of the synthesized DNA tetrahedral framework nucleic acid hydrogel, and mineralized DNA tetrahedral framework nucleic acid hydrogel Cap-gel is obtained; the Cap-gel can provide continuous calcium and phosphorus sources for bone regeneration and promote mineralization and maturation of new bones, so that the local mineralization microenvironment is improved, bone defect repair is promoted, and the Cap-gel has a good application prospect.

A process for producing a RNA vaccine comprising a plurality of epitopes specifically deduced from a target comprising the steps of: obtaining a plurality of synthetic DNA constructs in pool encoding (i) a plurality of different epitopes deduced from the said target, and of transcribing in vitro the said plurality of synthetic DNAs into a corresponding plurality of RNAs, wherein the said target is a peptide from an infectious agent or cancer neoepitopes specifically identified in one patient and having an amino acid sequence different, by at least one amino acid, from the amino acid sequences naturally present in normal cells of the patient.

The disclosure provides for methods of constructing synthetic chromosomes including the steps of providing host cells with an endogenous chromosome, transforming a cloning vector and a cloning cassette into the host cells, excising target genomic nucleic acids from the endogenous chromosome, recombining the excised target genomic nucleic acids with the cloning cassette via homologous recombination to form heterologous vectors comprising cloned sequences, extracting the heterologous vectors containing the cloned sequences from the host cells, digesting the heterologous vectors with a restriction endonuclease to release the cloned sequences from the heterologous vectors to provide released cloned sequences, and introducing the released cloned sequences, a centromere cassette, and a yeast artificial chromosomes or bacterial artificial chromosomes into a second host cell, such that the released cloned sequences, the centromere cassette, and the YAC or BAC recombine with one another via homologous recombination to produce the synthetic chromosome.

Embodiments disclosed herein provide methods of using synthetic DNA spike-ins (SDSIs) to detect, prevent, and quantify contamination in amplicon sequencing. These embodiments may, but are not limited to, reveal sample swaps, intra-batch contamination, and, on a larger scale, intra-laboratory contamination. Embodiments disclosed herein also provide synthetic DNA spike-ins for use in amplicon-based sequencing methods.

Provided herein are isolated DNA vectors comprising a heterologous gene, wherein the DNA vector is devoid of bacterial plasmid DNA and / or bacterial signatures, which can abrogate persistence in vivo.

The invention discloses a DNA joint compression coding method based on arithmetic coding and LZW coding, is applied to the field of molecular communication, and aims at solving the problem that an existing DNA compression algorithm can generate data redundancy and can cause extra data expenditure. The method comprises the following steps: performing original information compression by using improved LZW coding to generate a self-adaptive dictionary sequence; the generated dictionary sequence is further compressed by adopting arithmetic coding, and the arithmetic coding does not adopt original probability representation but adopts difference representation to reduce base pairs required by transmission probability, so that the problems of higher data redundancy and longer synthesized DNA chain in the existing DNA molecular communication are solved.

The present invention relates to a synthetic DNA construct comprising (A) a nucleic acid encoding transfer RNA and (B) a 5' leader sequence, wherein the 5' leader sequence contains a sequence motif for controlling the expression level of the transfer RNA.

The application discloses an aquifer delineation method and device based on synthetic DNA-thermal double tracing, and relates to the technical field of hydrogeophysical tracer tomography imaging, and the method comprises the following steps: obtaining DNA tracer travel time data and thermal tracer travel time data collected at an observation well after simultaneously injecting different types of synthetic DNA tracers and thermal tracers at multiple depths of an injection well of a target aquifer system; according to the DNA tracer travel time data and the thermal tracer travel time data, applying a SIRT inversion method based on a double-tracer mechanism to inversely calculate the permeability coefficient of the target aquifer, and further obtaining a water diffusion coefficient, a water storage rate and thermal characteristic parameters. The permeability coefficient of the target aquifer is inversely calculated through the double-tracer mechanism, and the aquifer heterogeneity can be accurately delineated.

The present disclosure describes systems and methods capable of fixing atmospheric nitrogen into bio-available nitrogenous compounds, including ammonia. Embodiments of the present disclosure are directed to synthetic DNA constructs encoding genes to allow release of bio-available nitrogenous compounds in nitrogen fixing diazotrophic organisms. Many of these constructs encode these genes in inducible and constitutive means, such that inducible embodiments can be activated at select times. Additional embodiments are directed to genetically engineered diazotrophs utilizing these constructs to produce bio-available nitrogenous compounds. Further embodiments are directed to methods to create these constructs and organisms as well as to use these constructs and organisms.

The application discloses a kind of photonic crystal fluorescence enhancement's printing synthetic DNA chip's cleaning effect verification method, comprising: S1, preparation photonic crystal microchip, form hydrophobic substrate and hydrophilic detection area;S2, build chip test platform, collect specific time's reaction cleaning liquid sample, and drop the sample to be measured on the surface of photonic crystal microchip;S3, heat photonic crystal microchip, after sample solution drying, the detection object is enriched in hydrophilic detection area;S4, the fluorescence spectrum signal that the detection object emits is detected by fluorescence spectroscopy, to realize the detection of detection object;S5, the cleaning effect of chip is judged using fluorescence intensity change.The verification method of the application can not only use fluorescence intensity change to judge the cleaning effect of chip, but also be suitable for trace detection under very low concentration condition, realize higher sensitivity than ordinary substrate such as glass, be favorable to reduce detection limit, realize more accurate analysis under low concentration.

Disclosed are methods of producing poxvirus-based vectors or recombinant poxvirus-based vectors from naturally derived, chemically synthesized DNA fragments, or a combination of naturally derived and chemically synthesized DNA fragments. One or more DNA sequences encoding one or more antigens, subunits or fragments thereof or other heterologous gene sequences are inserted in one or more poxvirus insertion sites in one or more DNA fragments. The methods include transfecting a host cell with one or more circular or linear DNA fragments such that a poxvirus or recombinant poxvirus is reconstituted in the host cell, the reconstituted poxvirus or recombinant poxvirus comprising the genome of a desired poxvirus. Also disclosed are poxviruses or recombinant poxviruses produced by the technology and uses thereof.

A synthetic DNA molecule comprising one segment encoding a tumor neoantigen or an epitope from an infectious agent under the control of a promoter for the transcription into a corresponding RNA molecule and a segment for the translation of the translated RNA molecule into a peptide.

Disclosed herein are mutants of bacteriophage Phi29 DNA polymerase with improved primer recognition, compared to the wild-type enzyme. Certain mutants comprise one or both of the mutations K64R or M97K. The provided mutants are capable of using more efficiently shorter and longer random synthetic DNA primers than wild-type Phi29 DNA polymerase, generating more amplification product in Multiple Displacement Amplification (MDA) reactions. The inventive mutants amplify human genomic DNA with less bias and better coverage in comparison to reactions carried out with wild-type Phi29 DNA polymerase.

The present invention relates a novel two or three component linear synthetic DNA vector system for use in methods of adeno-associated virus (AAV) vector production and methods of producing such a vector system. Such a system utilises linear, synthetic DNA molecules generated in an enzymatic in vitro manufacturing process, omitting the use of bacterial fermentation methods and is based on linear, double stranded and preferably covalently closed synthetic DNA.

Compositions and methods are provided for forming a single RNA polynucleotide from a plurality of DNA oligonucleotides in a single reaction chamber using combined reagents in a single step reaction. DNA polymerase, RNA polymerase and single stranded (ss) DNA oligonucleotides are combined where each DNA oligonucleotide has one or more sequence modules, wherein one sequence module in the first ss DNA oligonucleotide is complementary to a sequence module at the 3′ end of the second ss DNA oligonucleotide; and wherein a second module on the first ss DNA oligonucleotide is an RNA polymerase promoter sequence; and forming a single RNA polynucleotide, excluding the RNA promoter sequence, derived from the first and second DNA oligonucleotides.

One or more enzymes are used to repair damage in synthetic DNA molecules that encode digital information. The enzymes are included in a repair mixture containing one or more of DNA polymerase, DNA ligase, T4 Endonuclease, Endonuclease IV, Endonuclease VIII, and uracil glycosylase. The repair mixture may also contain one or more of a buffering solution, oxidized nicotinamide adenine dinucleotide (NAD+), and deoxyribose nucleoside triphosphates (dNTPs). The synthetic DNA molecules are incubated with the repair mixture for approximately four hours. Use of the repair solution allows recovery of the digital information from damaged DNA molecules.

This invention discloses a PCR bias correction method, a synthetic DNA sequence combination for a TCR-β immune repertoire, a primer combination for amplifying the TCR-β immune repertoire, a quantitative analysis method for a TCR-β immune repertoire from gDNA samples, a detection kit, and their applications. The PCR bias correction method can effectively correct biases generated during PCR amplification. The synthetic DNA sequence combination can effectively adjust the primer concentration ratio of multiplex PCR primer combinations. The primer combination can efficiently, quantitatively, and with minimal interference with each other amplify the TCR-β immune repertoire. The method can accurately quantify the immune repertoire at the DNA level.

A system for storing data on deoxyribonucleic acid (“DNA”) may include a receiver, a processor and / or a DNA synthesizer. The receiver may receive data files. The processor may segment the data files into a plurality of data packets. The processor may randomly select one or more packets from the plurality of data packets. The processor may combine the selected packets into an output. The processor may attach a random seed to the output. The processor may derive a sequence from the seeded output. The processor may identify the sequence as a valid sequence or a homopolymer. The processor may discard the sequence when the sequence is identified as a homopolymer. The DNA synthesizer may convert the sequence into a DNA quaternary sequence when the sequence is identified as a valid sequence. A DNA quaternary sequence may include DNA bases. The DNA synthesizer may synthesize and store the DNA sequence.

An isolated monomeric supercoiled synthetic circular expression vector comprises a promoter operably linked to a gene of interest, preferably a gene useful in therapy wherein the synthetic vector comp

Disclosed herein are mutants of bacteriophage Phi29 DNA polymerase that have improved primer recognition compared to the wild-type enzyme. Certain mutants contain one or both of the K64R or M97K mutations. The provided mutants are capable of using shorter and longer random synthetic DNA primers more efficiently than the wild-type Phi29 DNA polymerase, producing more amplification products in multiple displacement amplification (MDA) reactions. Compared to reactions performed with the wild-type Phi29 DNA polymerase, the mutants of the present invention amplify human genomic DNA polymerase with less deviation and higher coverage.

The invention discloses a biosensor for detecting AKI based on urine synthesized DNA and application, and belongs to the technical field of biomedicine. According to the invention, bio-orthogonal DNA is loaded on a molybdenum-doped tungsten oxide nanosheet with responsive degradation to ROS, and a nanosheet-bio-orthogonal DNA biosensor is constructed. The biosensor and a blank liposome are combined for detecting acute kidney injury (AKI), the blank liposome is used for inhibiting a reticuloendothelial system, so that the biosensor can enter more kidney metabolic pathways, in an AKI high ROS environment, the biosensor structure is damaged, biological orthogonal DNA is released, and finally the biosensor is discharged through urine. By enriching biological orthogonal DNA in urine, early diagnosis of AKI is finally realized. According to the invention, the problems of sensitivity and specificity in AKI detection can be solved, and a new technology is provided for early AKI screening in regions or countries with deficient resources.

Embodiments of the present invention generally relate to devices and methods related to deoxyribonucleic acid (DNA) synthesis. A device for synthesizing DNA is provided. The device includes a LED panel, the LED panel includes a backplane, the backplane having a backplane surface, the backplane surface having at least one contact pad disposed over the backplane surface, a plurality of LED, the plurality of LEDs disposed over the backplane surface, where each LED of the plurality of LEDs couples to a contact pad, pixel isolation (PI) structures, the PI structures disposed over the backplane surface and between the plurality of LEDs, the PI structures defining a plurality of wells, and a coating disposed over the LED panel, the coating operable to seal each well to hold a liquid.

The application discloses a biosensor for detecting AKI based on urine-synthesized DNA and application thereof, and belongs to the field of biomedical technology. The biosensor is constructed by loading bio-orthogonal DNA on molybdenum-doped tungsten oxide nanosheets which are responsive to ROS degradation. The biosensor is combined with blank liposomes to detect acute kidney injury (AKI), and the reticuloendothelial system is inhibited by the blank liposomes, so that the biosensor can enter the kidney metabolic pathway more. In the high ROS environment of AKI, the biosensor structure is destroyed, bio-orthogonal DNA is released, and finally excreted through urine. By enriching bio-orthogonal DNA in urine, early diagnosis of AKI is finally realized. The application can solve the sensitivity and specificity problems in AKI detection, and provide a new technology for early screening of AKI in resource-poor areas or countries.

Provided herein are methods for enzymatic production of de novo synthetic DNA molecules.

The present invention relates to a plasmid-free modular synthetic DNA templates (MSDT) and the manufacturing thereof. In particular, the invention relates to an MSDT to be used for in vitro mRNA transcription of antigen receptors and uses thereof in immunotherapy or immunotherapy screening and validation of candidate TCR pairs or CARs. Compared to existing constructs and methods, the present approach is plasmid-free, fast and inexpensive, allowing high throughput screening and validation of potential antigen receptor candidates in immunotherapy assays.