17 results about "Synthetic DNA" patented technology

The present invention relates to a method of selecting a polypeptide for display on a genetic display system according to activity, comprising the steps of: displaying the polypeptide on the genetic display system, screening a system for binding to a target, and selecting a member binding to the target; cloning a nucleic acid encoding the polypeptide from a member of the display system, and expressing the nucleic acid in an expression system to produce the polypeptide, or sequencing the polypeptide from a member of the display system, and cloning and expressing the polypeptide or a library of polypeptides using synthesis-based DNA; and determining the activity of the polypeptide in an assay.

The invention belongs to the technical field of biomedical materials, and particularly relates to mineralized DNA tetrahedral framework nucleic acid hydrogel as well as a preparation method and application thereof. The synthetic DNA tetrahedral framework nucleic acid hydrogel is subjected to mineralization treatment, so that calcium phosphate deposition is formed on the surface of the synthesized DNA tetrahedral framework nucleic acid hydrogel, and mineralized DNA tetrahedral framework nucleic acid hydrogel Cap-gel is obtained; the Cap-gel can provide continuous calcium and phosphorus sources for bone regeneration and promote mineralization and maturation of new bones, so that the local mineralization microenvironment is improved, bone defect repair is promoted, and the Cap-gel has a good application prospect.

The disclosure provides for methods of constructing synthetic chromosomes including the steps of providing host cells with an endogenous chromosome, transforming a cloning vector and a cloning cassette into the host cells, excising target genomic nucleic acids from the endogenous chromosome, recombining the excised target genomic nucleic acids with the cloning cassette via homologous recombination to form heterologous vectors comprising cloned sequences, extracting the heterologous vectors containing the cloned sequences from the host cells, digesting the heterologous vectors with a restriction endonuclease to release the cloned sequences from the heterologous vectors to provide released cloned sequences, and introducing the released cloned sequences, a centromere cassette, and a yeast artificial chromosomes or bacterial artificial chromosomes into a second host cell, such that the released cloned sequences, the centromere cassette, and the YAC or BAC recombine with one another via homologous recombination to produce the synthetic chromosome.

Embodiments disclosed herein provide methods of using synthetic DNA spike-ins (SDSIs) to detect, prevent, and quantify contamination in amplicon sequencing. These embodiments may, but are not limited to, reveal sample swaps, intra-batch contamination, and, on a larger scale, intra-laboratory contamination. Embodiments disclosed herein also provide synthetic DNA spike-ins for use in amplicon-based sequencing methods.

The present invention relates to a synthetic DNA construct comprising (A) a nucleic acid encoding transfer RNA and (B) a 5' leader sequence, wherein the 5' leader sequence contains a sequence motif for controlling the expression level of the transfer RNA.

The application discloses an aquifer delineation method and device based on synthetic DNA-thermal double tracing, and relates to the technical field of hydrogeophysical tracer tomography imaging, and the method comprises the following steps: obtaining DNA tracer travel time data and thermal tracer travel time data collected at an observation well after simultaneously injecting different types of synthetic DNA tracers and thermal tracers at multiple depths of an injection well of a target aquifer system; according to the DNA tracer travel time data and the thermal tracer travel time data, applying a SIRT inversion method based on a double-tracer mechanism to inversely calculate the permeability coefficient of the target aquifer, and further obtaining a water diffusion coefficient, a water storage rate and thermal characteristic parameters. The permeability coefficient of the target aquifer is inversely calculated through the double-tracer mechanism, and the aquifer heterogeneity can be accurately delineated.

The present disclosure describes systems and methods capable of fixing atmospheric nitrogen into bio-available nitrogenous compounds, including ammonia. Embodiments of the present disclosure are directed to synthetic DNA constructs encoding genes to allow release of bio-available nitrogenous compounds in nitrogen fixing diazotrophic organisms. Many of these constructs encode these genes in inducible and constitutive means, such that inducible embodiments can be activated at select times. Additional embodiments are directed to genetically engineered diazotrophs utilizing these constructs to produce bio-available nitrogenous compounds. Further embodiments are directed to methods to create these constructs and organisms as well as to use these constructs and organisms.

The application discloses a kind of photonic crystal fluorescence enhancement's printing synthetic DNA chip's cleaning effect verification method, comprising: S1, preparation photonic crystal microchip, form hydrophobic substrate and hydrophilic detection area;S2, build chip test platform, collect specific time's reaction cleaning liquid sample, and drop the sample to be measured on the surface of photonic crystal microchip;S3, heat photonic crystal microchip, after sample solution drying, the detection object is enriched in hydrophilic detection area;S4, the fluorescence spectrum signal that the detection object emits is detected by fluorescence spectroscopy, to realize the detection of detection object;S5, the cleaning effect of chip is judged using fluorescence intensity change.The verification method of the application can not only use fluorescence intensity change to judge the cleaning effect of chip, but also be suitable for trace detection under very low concentration condition, realize higher sensitivity than ordinary substrate such as glass, be favorable to reduce detection limit, realize more accurate analysis under low concentration.

A synthetic DNA molecule comprising one segment encoding a tumor neoantigen or an epitope from an infectious agent under the control of a promoter for the transcription into a corresponding RNA molecule and a segment for the translation of the translated RNA molecule into a peptide.

Disclosed herein are mutants of bacteriophage Phi29 DNA polymerase with improved primer recognition, compared to the wild-type enzyme. Certain mutants comprise one or both of the mutations K64R or M97K. The provided mutants are capable of using more efficiently shorter and longer random synthetic DNA primers than wild-type Phi29 DNA polymerase, generating more amplification product in Multiple Displacement Amplification (MDA) reactions. The inventive mutants amplify human genomic DNA with less bias and better coverage in comparison to reactions carried out with wild-type Phi29 DNA polymerase.

The present invention relates a novel two or three component linear synthetic DNA vector system for use in methods of adeno-associated virus (AAV) vector production and methods of producing such a vector system. Such a system utilises linear, synthetic DNA molecules generated in an enzymatic in vitro manufacturing process, omitting the use of bacterial fermentation methods and is based on linear, double stranded and preferably covalently closed synthetic DNA.

Compositions and methods are provided for forming a single RNA polynucleotide from a plurality of DNA oligonucleotides in a single reaction chamber using combined reagents in a single step reaction. DNA polymerase, RNA polymerase and single stranded (ss) DNA oligonucleotides are combined where each DNA oligonucleotide has one or more sequence modules, wherein one sequence module in the first ss DNA oligonucleotide is complementary to a sequence module at the 3′ end of the second ss DNA oligonucleotide; and wherein a second module on the first ss DNA oligonucleotide is an RNA polymerase promoter sequence; and forming a single RNA polynucleotide, excluding the RNA promoter sequence, derived from the first and second DNA oligonucleotides.

One or more enzymes are used to repair damage in synthetic DNA molecules that encode digital information. The enzymes are included in a repair mixture containing one or more of DNA polymerase, DNA ligase, T4 Endonuclease, Endonuclease IV, Endonuclease VIII, and uracil glycosylase. The repair mixture may also contain one or more of a buffering solution, oxidized nicotinamide adenine dinucleotide (NAD+), and deoxyribose nucleoside triphosphates (dNTPs). The synthetic DNA molecules are incubated with the repair mixture for approximately four hours. Use of the repair solution allows recovery of the digital information from damaged DNA molecules.

This invention discloses a PCR bias correction method, a synthetic DNA sequence combination for a TCR-β immune repertoire, a primer combination for amplifying the TCR-β immune repertoire, a quantitative analysis method for a TCR-β immune repertoire from gDNA samples, a detection kit, and their applications. The PCR bias correction method can effectively correct biases generated during PCR amplification. The synthetic DNA sequence combination can effectively adjust the primer concentration ratio of multiplex PCR primer combinations. The primer combination can efficiently, quantitatively, and with minimal interference with each other amplify the TCR-β immune repertoire. The method can accurately quantify the immune repertoire at the DNA level.

The invention discloses a biosensor for detecting AKI based on urine synthesized DNA and application, and belongs to the technical field of biomedicine. According to the invention, bio-orthogonal DNA is loaded on a molybdenum-doped tungsten oxide nanosheet with responsive degradation to ROS, and a nanosheet-bio-orthogonal DNA biosensor is constructed. The biosensor and a blank liposome are combined for detecting acute kidney injury (AKI), the blank liposome is used for inhibiting a reticuloendothelial system, so that the biosensor can enter more kidney metabolic pathways, in an AKI high ROS environment, the biosensor structure is damaged, biological orthogonal DNA is released, and finally the biosensor is discharged through urine. By enriching biological orthogonal DNA in urine, early diagnosis of AKI is finally realized. According to the invention, the problems of sensitivity and specificity in AKI detection can be solved, and a new technology is provided for early AKI screening in regions or countries with deficient resources.

Embodiments of the present invention generally relate to devices and methods related to deoxyribonucleic acid (DNA) synthesis. A device for synthesizing DNA is provided. The device includes a LED panel, the LED panel includes a backplane, the backplane having a backplane surface, the backplane surface having at least one contact pad disposed over the backplane surface, a plurality of LED, the plurality of LEDs disposed over the backplane surface, where each LED of the plurality of LEDs couples to a contact pad, pixel isolation (PI) structures, the PI structures disposed over the backplane surface and between the plurality of LEDs, the PI structures defining a plurality of wells, and a coating disposed over the LED panel, the coating operable to seal each well to hold a liquid.

The application discloses a biosensor for detecting AKI based on urine-synthesized DNA and application thereof, and belongs to the field of biomedical technology. The biosensor is constructed by loading bio-orthogonal DNA on molybdenum-doped tungsten oxide nanosheets which are responsive to ROS degradation. The biosensor is combined with blank liposomes to detect acute kidney injury (AKI), and the reticuloendothelial system is inhibited by the blank liposomes, so that the biosensor can enter the kidney metabolic pathway more. In the high ROS environment of AKI, the biosensor structure is destroyed, bio-orthogonal DNA is released, and finally excreted through urine. By enriching bio-orthogonal DNA in urine, early diagnosis of AKI is finally realized. The application can solve the sensitivity and specificity problems in AKI detection, and provide a new technology for early screening of AKI in resource-poor areas or countries.