69 results about "DNA formation" patented technology

Methods and compositions for producing hRNA, e.g., shRNA, expression modules for specific target nucleic acids are provided. In the subject methods, an initial nucleic acid, e.g., dsDNA, synthetic DNA, etc., corresponding to the target nucleic acid of interest is converted to an intermediate nucleic acid. The resultant intermediate nucleic acid, following an optional size modification step, is then converted to a linear dsDNA that includes at least one copy of the hRNA expression module of interest, or a precursor (i.e., pro-shRNA expression module) thereof, where in certain embodiments conversion may include amplification. Also provided are reagents, systems and kits for use in practicing the subject methods. The subject methods and compositions find use in a variety of different applications.

Aerosol delivery of nucleic acids to the lungs using viral vectors, polymers, surfactants, or excipients has been described. Compositions for intranasal administration are described that contain nucleic acids without viral or plasmid vectors and with little to no polymers, surfactants, or excipients. In one embodiment, the composition for intranasal delivery consists essentially of at least one nucleic acid and an aqueous solution. Suitable nucleic acids for intranasal delivery include, but are not limited to, dsDNA, dsRNA, ssDNA, ssRNA, short interfering RNA, micro-RNA, and antisense RNA. Methods for treatment, diagnosis, or prevention of at least one symptom or manifestation of a lung disease are also described consisting of administration by intranasal delivery an effective amount of a composition containing a nucleic acid. The composition may be formulated as a liquid or aerosol or other acceptable formulation for intranasal administration.

Methods and compositions are provided for performing isothermal amplification of a nucleic acid target employing probes characterized by having a masked RNA polymer promoter unable to bind to a complementary initiator oligonucleotide and RNA polymerase and initiate transcription, a dsDNA sequence which when invaded by the target nucleic acid exposes the masked promoter to initiate transcription, and a template sequence, a portion of which is normally included in the dsDNA region, which when copied produces a product that can reinitiate the process of invading the dsDNA region and initiating transcription of another copy.

An antitumor agent containing, in combination, at least one kind of antitumor agent selected from the group consisting of an antitumor agent that forms a cross-link with DNA and shows an antitumor effect, an antimetabolite antitumor agent and a taxane antitumor agent, and a histone deacetylase inhibitor. According to the present invention, an antitumor agent causing reduced side effects and having a superior antitumor activity can be provided.

A method for producing a single stranded DNA (ssDNA) molecule of a defined length and sequence is disclosed. This method enables the preparation of, inter alia, probes of greater length than can be chemically synthesized. The method starts with a double stranded molecule, such as genomic, double stranded DNA (dsDNA) from any organism. A fragment of the starting molecule (dsDNA) is amplified by specific primers engineered to introduce cleavage sites on either side of the desired sequence. Cleavage steps on the amplified, engineered fragment are combined with a phosphate removal step, thereby creating a construct that can be digested with an exonuclease without damage to the desired ssDNA. Probes, which hybridize with large gaps between the ends of the probes, are also disclosed.

The present invention relates generally to compositions and methods using mammalian, dsDNA (Double Stranded Deoxyribonucleic Acid) vaccination for the induction and maintenance of regulator suppressor T cells resulting in suppression of non infectious, and post infectious, inflammatory, allergic, auto-immune, vasculitic, certain degenerative vascular, and graft versus host diseases, with or without the use of IL-10, and with or without the use or TGFβ, with or without the use of anti-IL 6 receptor antibody, anti TNF antibody and or Plasmapheresis, IVIG, Corticosteroids, Methotrexate, Bromocriptine, and or vitamin D analogues.

The invention discloses a self-immunity hepatitis detection protein chip and a kit thereof. The chip and the kit can be used for simultaneously detecting 12 immunity protein markers: ANA (dsDNA, RNP, Sm, SS-A/Ro, SS-B/La, Sc1-70, Jo-1), SMA, LKM-1, SLA/LP, F-actin and AMA. The invention overcomes the shortcomings of the current products that the detection index is single and incomplete. The invention provides a detection method which has a complete diction result and a simpler process.

Methods and compositions are provided for forming complexes intracellularly between dsDNA and oligomers of heterocycles, aliphatic amino acids, particularly omega-amino acids, and a polar end group. By appropriate choice of target sequences and composition of the oligomers, complexes are obtained with low dissociation constants. The formation of complexes can be used for modifying the phenotype of cells, either prokaryotic or eukaryotic, for research and therapy.

A computer-implemented method of analysis of melt curve data comprising the steps of: (a) parameter model fitting the melt curve data; (b) performing a principal component analysis of the melt curve data; and (c) utilizing the principal components for clustering the melt curve data into groups. Such a method allows an efficient analysis of variations in melt curve shape and position and allows to statistically quantify these variations for both supervised and unsupervised data sets. Current melt analysis methods neither allow for statistical measures of each unknown nor do they allow for the determination of unsupervised data sets (i.e., unknown number of groups present). Particularly, the method according to the invention can be advantageous for identifying a specific sequence of dsDNA in a sample after performing a polymerase chain reaction (PCR).

The invention discloses lupus erythematosus detection protein chip and kit thereof. The chip comprises a substrate, protein markers distributed in an array type and control point coatings, wherein the markers and the control point coatings are seven antigen markers, positive controls and negative controls comprising dsDNA (double-stranded deoxyribonucleic acid), ssDNA (single stranded deoxyribonucleic acid), Histon, SS-A(Ro60), SS-B(La), Sm (smith) and RNP (ribonucleoprotein)/Sm which are uniformly distributed on the substrate in a dot matrix mode. The invention has comprehensive and appropriate detection indexes and consistent applied reaction conditions, can detect a plurality of indexes at a time, is convenient and quick, greatly improves the detection efficiency and reduces the detection cost. The invention can be suitable for aided diagnosis of suspected lupus erythematosus disease people.

The invention discloses a color protein chip which substrate is BCIP/NBT. The invention is composed of a substrate, an array, 12 antigens coated on the array, negative contrast, positive contrast and blank contrast, wherein the substrate is a glass slide decorated by APES, the kinds and point sample densities of the 12 antigens are 520ug/ml ANA, 465ug/ml Ro-60, 530ug/ml La/SSb, 530ug/ml Jo-1, 525ug/ml Scl-70, 520ug/ml Sm, 615ug/ml Ro-52/SSa, 340ug/ml RF, 465ug/ml CCP, 410ug/ml u1RNP, 490ug/ml CENP-B, 580ug/ml dsDNA. The invention uses the alkali phosphatase coloring method whose substrate is BCIP/NBT as the final check signal of protein chip to be widely used for self antibody check, while the check sensitivity has higher coincidence rate than prior art, with reduced check cost and time. The invention can be used in clinical check, sanitary supervision, regular sanitary supervision and scientific research or the like.

A process for high-flux screening, capturing and separation of target moleculae from complex substance (Chinese medicine or chemical mixer) includes preparing a ds DNA microarray chip to make its probe have a binding sites of dsDNA bindin, measuring the affinity of the sites, creating the standard affinity parameter system, mixing the microarray chip with said complex substance, reacting, detecting the affinity variation, screening the varied targets, linking them to the surface of chromatographic column, filling the complex substance in it, capturing the effective moleculae by probes, eluting and collecting the effective moleculae.

The invention discloses a nucleic acid detection method based on surface plasmon resonance technology. First, graphene oxide is electrodeposited on the surface of a bare SPR chip, and then the activated molecular layer of perylene derivatives grafted with aminotriacetic acid is adsorbed through conjugation. Assemble the 5′ end biotinylated capture cDNA probe as the sensing interface to detect the target tDNA, and after realizing the binding and capture of tDNA, add the 3′ end biotinylated response rDNA to form a long chain dsDNA and expose its biotin molecules , combined with avidinated horseradish peroxidase to complete the assembly, and finally add aniline and hydrogen peroxide mixed solution, use horseradish peroxidase to catalyze the aniline polymerization deposition reaction to form polyaniline, and realize the detection of target nucleic acid according to the SPR signal. The present invention uses two-dimensional nanomaterials for "bottom-up" construction, and forms an SPR sensing platform with double-sensitized signals with a top-mounted "top-down" gravimetric reactor, which can achieve specificity and sensitivity to target DNA. Sensitive detection, the detection limit can reach femtomolar level.

A process for preparing the unimolecular ds DNA microarray on the surface of solid carrier includes chemically synthesizing target oligonucleotide and universal oligonucleotide, renaturation of them, linking them to the surface of solid carrier to become oligonucleotide microarray, on-chip polymerase elongation reaction to become bimolecular dsDNA microarray, modifying to become ssDNA microarray, renaturation to form hairpin structure, and on-chip polymerase elongation reaction.

Methods and compositions are provided for forming complexes between dsDNA and novel DNA-binding polymers comprising N-terminal thiophene-containing moieties which exhibit selectivity for T-A base pairs. By appropriate choice of target sequences and DNA-binding polymers, complexes comprising polymer-DNA are obtained with high association constants. The formation of complexes can be used for identification of specific dsDNA sequences, for inhibiting gene transcription, and as a therapeutic for inhibiting proliferation of undesired cells or modulation of expression of specific genes.

Disclosed is a transcription factor protein detection method through exonuclease III digestion FRET-dsDNA micro array chips, wherein the expression and activation levels of the transcription factor are analyzed through the following steps: (1) preparing FRET-dsDNA micro array chips, (2) reacting the transcription factor with FRET-dsDNA micro array chips, (2) reacting the exonuclease III with FRET-dsDNA micro array chips, (1) proceeding detection and analysis to the FRET-dsDNA micro array chips.

Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5′ of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.

Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel.