47results about How to "High sensitivity detection" patented technology

The invention belongs to the technical field of optical fiber gas sensing, and discloses an optical fiber photoacoustic gas sensing system and method suitable for a low-pressure environment. The system comprises a programmable logic gate array circuit, a distributed feedback laser, a laser transmission optical fiber, an optical fiber photoacoustic sensing probe, a super-radiation light-emitting diode light source, an optical fiber circulator and a spectrograph. The air pressure is monitored according to the linear relation between the resonance frequency of the cantilever beam and the ambient air pressure, the characteristic that the acoustic sensitivity at the resonance position of the cantilever beam is enhanced under the low air pressure is utilized to make up for signal attenuation in the photoacoustic excitation process, and the sensitivity is corrected in combination with the air pressure measured by the cantilever beam. Therefore, high-precision and high-sensitivity detection of trace gas in a low-pressure environment is realized. The system only adopts a single cantilever beam and a single demodulator, has the functions of gas pressure and gas concentration detection, and can realize accurate detection of trace gas in a gas pressure change environment. And an all-fiber scheme is adopted, so that intrinsic safety is realized, and remote measurement and multi-point network group detection are easy.

The invention belongs to the technical field of molecular biology, and particularly relates to a pseudo-ginseng component rapid detection method based on a Proofman-LMTIA technology. According to the method, a pseudo-ginseng ITS2 sequence is taken as a specific target, and high-specificity and high-sensitivity detection of pseudo-ginseng components is realized through a designed LMTIA primer and a Proofman probe. Compared with the traditional detection technology, the invention has the advantages of simplified primer design, low-cost equipment requirement, rapid detection (only about 30 minutes) and wide application range. The method is not only suitable for detection of raw materials such as pseudo-ginseng seeds, seedlings, roots and rhizomes, decoction pieces and the like, but also can be used for authenticity identification of terminal products such as food, medicines, health care products and the like containing pseudo-ginseng components. Through innovative application of the Proofman-LMTIA technology, an effective solution is provided for rapid and accurate identification of pseudo-ginseng and derivative products thereof, and the method has important market application prospects and social benefits.

The invention discloses a laser absorption spectrum system for random noise modulation, which relates to the technical field of trace gas detection of absorption spectrum technology, and is characterized in that a signal generation module generates random noise and low-frequency triangular waves with different powers, different bandwidths and different distribution characteristics; laser generated by the light source module is modulated through random noise and low-frequency triangular waves; laser generated by the light source module enters the light flux cell, namely the annular optical multi-pass cell and reciprocates; the signal processing module receives emergent light of the light flux cell and generates a first feedback signal and a second feedback signal according to the emergent light of the light flux cell; the signal generation module adjusts random noise according to the first feedback signal; the light source module adjusts the laser incident angle according to the second feedback signal. According to the invention, random noise modulation is carried out on the light source, interference noise of the system is suppressed, and under the condition that the physical size and the structure of the light flux cell are ensured, the distribution number of mirror light spots is increased, and the effective absorption optical path of gas in the light flux cell is improved, so that high-sensitivity detection of trace gas is realized.

The invention discloses a hydroxyproline rapid detection molecularly imprinted electrochemical sensor as well as preparation and application thereof, and relates to the field of quality safety monitoring of medicinal plants. The preparation method of the molecularly imprinted electrochemical sensor comprises the following steps: preparing the Fc-MWCNTs composite material, preparing the Fc-MWCNTs / GCE, and preparing the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE. According to the invention, MWCNTs with good conductivity and Fc which has good stability and oxidation-reduction property and is used as an excellent electron transfer medium are used as electrode substrate materials; and the Fc-MWCNTs and the MWCNTs are covalently bonded through an amide reaction to prepare the composite material Fc-MWCNTs which has excellent electron transfer medium characteristics, low potential reproducibility and strong stability and can remarkably improve the electron transfer rate between the electrode surface and an electrolyte. The modified composite material Fc-MWCNTs is modified on the surface of GCE, so that the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE with high sensitivity, high selective recognition and fast detection is constructed, and the molecularly imprinted electrochemical sensor is used for detecting an index component Hyp of cornu cervi pantotrichum.

The embodiment of the invention belongs to the technical field of electrochemical gas sensors, and relates to a preparation method of a counter electrode of an NO sensor, which comprises the following steps: adding a polymer adhesive into an organic solvent to prepare a polymer adhesive mother solution; mixing manganese dioxide powder with the polymer adhesive mother liquor, and stirring to form uniform slurry; coating a substrate with the slurry, and performing drying treatment to form a counter electrode plate; and putting the counter electrode plate into an environment with reducing gas or inert gas to carry out high-temperature reduction treatment so as to improve the oxygen vacancy concentration, thereby obtaining the counter electrode of the NO sensor. The invention further provides an NO sensor and an NO detection method. The counter electrode of the NO sensor and the NO sensor prepared by the embodiment of the invention have the advantages of low cost and high anti-interference capability while keeping high oxygen reduction catalytic activity, and are particularly suitable for high-selectivity and high-sensitivity detection of NO gas in the fields of environmental monitoring, medical diagnosis and the like.

The invention relates to the technical field of cell apoptosis detection, in particular to a kit for cell apoptosis detection and a product thereof. The Annexin V-FITC provided by the invention can well realize the detection of apoptosis, has obvious linear correlation with the apoptosis rate, and can realize the high-sensitivity detection of the occurrence of apoptosis. The method and the kit for detecting cell apoptosis can effectively identify phosphatidylserine exposed on the outer surface of apoptotic cells, and have more detection advantages compared with Annexin V full length. The sensitivity and the cell apoptosis rate of the detection method have a good linear relationship, and the detection method has a relatively great application prospect.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

The invention belongs to the technical field of biosensing and microelectronics, and relates to a preparation method and application of an extended gate field effect transistor for thrombin instant detection. The expanded gate field effect transistor is modified by the Prussian blue / platinum nanoparticle composite material, the electrochemical sensing performance of the field effect transistor is greatly improved, and the expanded gate field effect transistor prepared based on the composite material shows excellent anti-interference capability and recognition capability. The expanded gate field effect transistor has the advantages of being low in cost, easy to replace and the like, the preparation process is simple and controllable, large-scale and productization production is easy to achieve, the expanded gate field effect transistor based on the Prussian blue / platinum nanoparticles can achieve rapid recognition and instant detection of thrombin, the blank of thrombin detection products in the market at present is filled, and the application prospect is wide. And the detection efficiency of the blood coagulation index is greatly improved.

The present application relates to a kind of biological base micro needle sensor-based crop health nondestructive testing method, belong to agricultural information technology and biosensor field.The method is intelligently analyzed to crop surface microstructure by convolutional neural network, realize the accurate arrangement of microneedle array and customized nondestructive adaptation.Microneedle multimodal sensing unit can synchronously real-time acquisition crop internal multidimensional physiological parameters, and signal quality is improved using adaptive filtering and standardization processing.Combined with multivariate anomaly detection algorithm of self-supervised and contrast learning, health status is unsupervised clustering and early abnormal screening, and environmental and management information is fused, realize whole plant multiscale health evaluation, abnormal risk distribution analysis and dynamic traceability.System can intelligently output differentiated farm work suggestions, realize the high accuracy, nondestructive, real-time monitoring and intelligent management of crop health.

This invention belongs to the field of medical testing technology, specifically relating to a lateral flow test strip for ultra-high sensitivity detection of pathogens and its application. The lateral flow test strip provided by this invention uses polydopamine nanoparticles modified with bacterial antibodies as signal probes. Multiple detection lines are set on the NC membrane, and the detection lines are obtained by scribing with a bacterial antibody solution as ink. This invention replaces Au NPs in classic LFIAs with PDA NPs. Relying on the high extinction coefficient, affinity for bacteria, and stability of antibody binding of PDA NPs, a two-order-of-magnitude improvement in sensitivity is achieved when using LFIAs to detect bacteria. Simultaneously, utilizing the high extinction coefficient of PDA NPs, their signals can be identified and statistically analyzed by ImageJ software even when invisible to the naked eye. Combined with multiple test strips, this improves target capture efficiency, thereby achieving highly sensitive detection of pathogenic bacteria.

The invention belongs to the technical field of electrochemical detection, and particularly relates to an enrofloxacin aptamer sensor based on a screen-printed electrode and a rapid detection method. The preparation method comprises the following steps: firstly preparing a Fe / Co two-dimensional MOF nanosheet with a lamellar structure by using a room temperature stirring method, then incubating with an enrofloxacin aptamer to prepare a nanosheet / aptamer probe, further incubating with enrofloxacin, mixing with an acetic acid-sodium acetate buffer solution, o-phenylenediamine and H2O2 to form a catalytic reaction system, and by combining with a screen-printed electrode-differential pulse voltammetry, detecting the content of the enrofloxacin. The enrofloxacin concentration of a sample to be detected is rapidly tested by drawing a quantitative analysis standard working curve, and rapid, simple and high-sensitivity detection of enrofloxacin is realized. The method is simple and rapid to operate, does not need expensive reagents, large-scale instruments and professional operators, is high in sensitivity, and can realize on-site, rapid and high-sensitivity quantitative detection of enrofloxacin in aquatic products such as grass-roots fish and shrimps in combination with a portable electrochemical detector.

The present invention belongs to the field of antibody technology, and in particular, relates to anti-human granulocyte-macrophage colony stimulating factor antibodies, antibody pairs and their applications. The antibody is a first antibody or a second antibody, and the amino acid sequences of CDR1-3 on the light chain variable region of the first antibody are shown as SEQ ID NO.3-5, respectively, and the amino acid sequences of CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.8-10, respectively; the amino acid sequences of CDR1-3 on the light chain variable region of the second antibody are shown as SEQ ID NO.13-15, respectively, and the amino acid sequences of CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.18-20, respectively. The present invention provides antibodies with good specificity and high affinity for human GM-CSF, which can effectively improve the accuracy, reliability and sensitivity of immunoassay results, and provide a more accurate and reliable antibody tool for detecting GM-CSF protein levels.

This invention provides a rapid colorimetric reagent for the detection of manganese and iron ions. The reagent consists of carbon dots, a 3,3′,5,5′-tetramethylbenzidine solution, and a phosphate buffer solution. The reagent itself is a brown, transparent solution. When manganese ions are added to the 3,3′,5,5′-tetramethylbenzidine solution and phosphate buffer solution, it turns blue (visible to the naked eye) upon irradiation with a 365 nm UV lamp; upon contact with iron ions in the 3,3′,5,5′-tetramethylbenzidine solution, it turns dark green (visible to the naked eye). This reagent exhibits an extremely low detection limit and good selectivity. The detection process does not require complex analytical equipment, enabling rapid trace detection of manganese and iron ions. The reagent is simple to prepare, overcoming the shortcomings of cumbersome and expensive synthesis of manganese and iron ion detection probe molecules, and providing effective technical support for trace detection in food safety.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The invention discloses a double-shaft type optical speed detector and a calibration method, and particularly relates to the technical field of detectors. The double-shaft type optical speed detector comprises a double-optical-path detection module, a sample adaptation module, a speckle manufacturing and detecting module, a data processing and calibration module and a display and control module. According to the double-shaft type optical speed detector and the calibration method, the light path building process is simplified through the arrangement of the integrated light path module, a user can carry out detection only through simple operation, the operation convenience is improved, the detection capacity for sample characteristic changes is effectively enhanced through the arrangement of the double-light-path detection module, and the detection accuracy is improved. The high-sensitivity detection is realized, the sample adaptation module is arranged to adapt to various samples, the universality of the device is improved, and meanwhile, the speckle manufacturing and detection module is arranged to realize high-quality and high-adaptability speckle manufacturing and detection. And the data processing and calibration module and the display and control module ensure the accuracy of data and the intuition of result display.

The invention relates to the technical field of electrochemistry, in particular to a preparation method and application of a copper-silver bimetal organic framework compound / rhodium electrode for electrochemically detecting total nitrogen, and the preparation method comprises the following steps: A, preparing terephthalic acid, silver nitrate and a solvent into a mixed solution, the foamy copper is placed in the mixed solution for a hydrothermal reaction, and a copper-silver bimetal organic framework compound is obtained; and B, forming a rhodium film layer outside the copper-silver bimetal organic framework compound in the step A, and finally annealing and removing impurities to obtain the copper-silver bimetal organic framework compound / rhodium electrode material, when the electrode is applied to total nitrogen detection, the electrode has stronger volt-ampere response to nitric acid, is good in stability, and can realize high-sensitivity and rapid detection of total nitrogen in a water body.

The invention relates to the technical field of biomedical engineering, and discloses a urine microalbumin detection method based on a molecularly imprinted polymer and nano enzyme, which comprises the following steps: firstly, selecting C-terminal nonapeptide of ALB as an epitope, introducing lysine and combining with fructose to synthesize a saccharification epitope template; a microwell plate capable of specifically recognizing epitope imprinting of ALB is prepared through a boron affinity anchoring epitope controllable directional surface imprinting method, then boric acid modified ferroferric oxide nano-enzyme with high catalytic activity is synthesized and selectively combined with a sugar chain of ALB through the boron affinity effect, then a mobile phone detection technology is introduced, and the ALB epitope imprinting specific recognition microwell plate is obtained. And establishing a sandwich immune sandwich method based on the molecularly imprinted polymer and the nano-enzyme. According to the method, antibodies and biological enzymes are not needed, the detection cost is reduced, meanwhile, professionals and equipment are not needed, instant detection can be achieved in combination with a smart phone, the detection result is obtained rapidly, a rapid, specific, sensitive and convenient detection tool is provided for early diagnosis of CKD, and the method has important clinical application value.

The invention relates to the technical field of salmonella detection, and provides a photonic crystal fluorescence sensor based on recombinase polymerase amplification and a CRISPR / Cas12a system, and the photonic crystal fluorescence sensor comprises a biosensing platform and a photonic crystal array; the reaction components of the biosensing platform comprise an RPA reagent, an upstream primer, a downstream primer, Cas12a protein, crRNA, an ssDNA probe and a buffer solution; the photonic crystal array is mainly constructed by a PDMS substrate, polystyrene microspheres, ethanol and a microwell plate analyzer; the detection sensitivity of the salmonella is obviously improved.

The invention discloses a surface potential sensor taking an allosteric transcription factor as a recognition molecule as well as a preparation method and application of the surface potential sensor, and belongs to the technical field of surface potential detection. The surface potential sensor comprises an electrode, desoxyribonucleic acid and an allosteric transcription factor, the electrode is sequentially connected with desoxyribonucleic acid and an allosteric transcription factor; the number of base pairs in the nucleotide sequence of the deoxyribonucleic acid is more than 30. An allosteric transcription factor is used as a recognition molecule, so that the type of the recognition molecule can be changed according to the change of a target object, and the application range is wide; the construction method of the surface potential sensor is simple, and when the surface potential sensor is adopted as a working electrode for surface potential detection, rapid and high-sensitivity detection on a target object can be achieved.

The invention discloses a method and post-treatment fluid for reducing squamous cell carcinoma antigen detection background, and is characterized in that the method comprises the following steps: step 1, adding a sample, magnetic beads containing a coated antibody, a sample diluent and a labeled antibody labeled with acridinium ester, incubating, and executing a cleaning step; step 2, adding a post-treatment liquid, incubating, and executing a cleaning step; step 3, adding a substrate solution, entering a photometric module, and measuring the number of photons; wherein the post-treatment liquid comprises the following raw material components: a basic buffer solution, a nonionic surfactant, an alkali metal chloride, a metal ion chelate and an anionic surfactant. The method provided by the invention is simple and convenient to operate, and can improve the detection accuracy and maintain high sensitivity.

[Problem] To provide a technique with which it is possible to detect a target substance in a sample with high sensitivity and at low cost compared with conventional techniques. [Solution] A method for detecting a target substance, the method including forming a complex by sandwiching the target substance between a first capture substance immobilized in a solid phase and a second capture substance labeled with a marker in a first reaction field, separating a portion including the marker from the complex, and detecting the target substance in a second reaction field. The liquid is filled in the tubular channel by a centrifugal force, and the marker-containing portion is detected by scattered light scattered by the marker-containing portion.

The invention discloses a wind driven generator transmission chain vibration detection method based on an echo wall mode microcavity, and relates to the technical field of wind power generation. The wind driven generator transmission chain vibration detection method comprises the following steps: guiding a laser light source into the echo wall mode microcavity based on an optical fiber coupling technology; a vibration response signal at the position of a transmission chain of the wind driven generator is obtained by using the echo wall mode microcavity; and converting the vibration response signal into a digital signal by using a signal processing technology, analyzing and processing the digital signal by combining a digital signal processor, and obtaining vibration information at the position of the transmission chain of the wind driven generator based on a processing result. According to the wind driven generator transmission chain vibration detection method based on the echo wall mode microsphere cavity, the vibration condition of the wind driven generator transmission chain can be detected and analyzed in a high-precision and high-sensitivity mode, and meanwhile the method has the advantages of being capable of resisting interference and adapting to severe environments.

The invention belongs to the technical field of feed detection, and specifically relates to a rapid detection method for pesticide, veterinary drug and fungal toxin residues in feed, comprising the following steps: using a mixed extract of methanol, acetonitrile and isopropanol to extract a sample under alkaline conditions and acidic conditions in turn, mixing, solid phase extraction adsorbent (including N-vinylbenzene modified ferroferric oxide functionalized magnetic nano-adsorbent material and octadecyl silica gel C 18 After purification by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) in multiple reaction monitoring scanning mode, quantification is performed by external standard method or internal standard method. It can quickly and accurately detect 420 pesticides, 163 veterinary drugs and their metabolites and 13 mycotoxins in feed at the same time, with a quantitative limit of 0.01 μg / kg to 50 μg / kg, which is of great significance for ensuring food safety, public health and the sustainable development of animal husbandry.

The present invention is a method for detecting hepatitis E virus infection, comprising a detection step of detecting anti-hepatitis E virus antibodies in a sample by using, as an antigen polypeptide, at least one polypeptide selected from the group consisting of the following (a) to (c): (a) a polypeptide consisting of an amino acid sequence which includes amino acids located at position 28 to 175 in the amino acid sequence set forth in any one of SEQ ID NOs: 1 to 4 and which has a full length of 200 residues or less that are contained in the amino acid sequence set forth in the sequence number; (b) a polypeptide consisting of an amino acid sequence in which one or more amino acids are substituted, deleted, inserted, and / or added in the amino acid sequence which includes amino acids located at position 28 to 175 in the amino acid sequence set forth in any one of SEQ ID NOs: 1 to 4 and which has a full length of 200 residues or less that are contained in the amino acid sequence set forth in the sequence number; and (c) a polypeptide consisting of an amino acid sequence having 80% or more homology with the amino acid sequence which contains amino acids located at position 28 to 175 in the amino acid sequence set forth in any one of SEQ ID NOs: 1 to 4 and which has a full length of 200 residues or less that are contained in the amino acid sequence set forth in the sequence number.

The invention relates to preparation and application of a hydrogen sulfide (H2S) near-infrared fluorescent probe. The structural formula of the probe is # imgabs0. The invention provides a preparation method for synthesizing the fluorescent probe by taking benzoindole-thioxanthene dye, 4-chloro-7-nitrobenzofurazan and the like as raw materials. The fluorescent probe is a hydrogen sulfide fluorescent probe with large Stokes shift and long near infrared emission; firstly, the fluorescent probe shows good sensitivity to H2S, the linear range is 0.5-30 [mu] M, and the detection limit is 0.2 [mu] M; secondly, the fluorescent probe shows very high selectivity on H2S, and is not influenced by other metal ions, active nitrogen, active oxygen, amino acid, active sulfur and biological mercaptan; moreover, the fluorescent probe can quickly act with H2S, and the response time is within 10 minutes; in addition, the fluorescent probe can also be applied to detection of H2S content in living cells.

The invention discloses a fluorescent self-early-warning anticorrosive coating as well as a preparation method and application thereof. The coating comprises epoxy resin coupled with probe molecules. The fluorescence emission wavelength of the probe molecule is located in a near-infrared region, and the fluorescence intensity can be enhanced under an acidic condition, so that the detection reliability of the self-early-warning anticorrosive coating and the detection depth of an early-warning signal are improved; according to the acidic pH response type fluorescent self-early-warning anticorrosive coating disclosed by the invention, coating cracks in different shapes can be accurately detected; the acidic pH-responsive fluorescent self-early-warning anticorrosive coating provided by the technical scheme of the invention has good stability, can perform long-time early warning on the anticorrosive coating, and provides long-term protection for ships.

The invention relates to the field of optical fiber sensing and the field of deep learning, in particular to an air drum structure and a low-frequency acoustic signal detection device and method based on the air drum structure, which combine the advantages of an F-P cavity formed by double-fiber Bragg gratings, a conical fiber and the air drum structure and fully play the advantages of simple structure, high sensitivity, easy multiplexing and the like of the optical F-P cavity. Round diaphragms with different areas and different corresponding resonant frequencies are prepared by considering the characteristic that the fiber Bragg grating only reflects specific wavelength and the natural high sensitivity characteristic of the conical fiber and combining the narrow-band filtering advantage of the round diaphragms, so that the empty drum structure detects sound waves with different frequencies, and the detection accuracy is improved. The frequency of the external sound wave can be directly obtained without additional Fourier transform and filtering operation, and intelligent autonomous identification of the target sound signal is realized; compared with a traditional low-frequency sound signal sensing system, the system is more sensitive, compact and efficient, and has the advantages of narrow-band filtering, real-time spectrum extraction, autonomous target recognition and the like.

The invention relates to the field of chimeric cell proportion detection, in particular to a method for highly sensitively detecting the proportion of chimeric cells after bone marrow transplantation. Aiming at the condition that a bone marrow transplantation donor and a patient are heterosexual, the method comprises the following steps: firstly, selecting a gender specific gene as a target, then analyzing the transcriptional level of the gender specific expression gene in a blood sample of the patient before and after transplantation through a real-time fluorescence quantification method, and finally calculating to obtain the residual proportion of the patient cells. The detection method provided by the invention can be used for evaluating the chimeric state of a heterologous hematopoietic stem cell transplantation patient and monitoring the implantation state, relapse or MRD of donor stem cells in a receptor so as to guide clinics to take corresponding treatment measures in time.

The application discloses a long-afterglow nano carrier and a preparation method and application thereof, and belongs to the field of biomedical materials. The long-afterglow nano carrier comprises a near-infrared luminescent center, an organic mesoporous silica layer and an autophagy inhibitor; the organic mesoporous silica layer is coated on the outer layer of the near-infrared luminescent center, and the autophagy inhibitor is loaded in the mesopores of the organic mesoporous silica layer; the near-infrared luminescent center comprises a transition metal element; the organic mesoporous silica layer is prepared by co-condensation of an organic silane precursor and an inorganic silicon precursor, wherein the organic silane precursor is an organic silane containing a tetrasulfide bond, and the inorganic silicon precursor is a silicate compound. The carrier can specifically respond to excessively high concentration of hydrogen peroxide in a tumor microenvironment, and accurately release the loaded autophagy inhibitor; the release process can be observed by positioning with the aid of a high signal-to-noise ratio afterglow signal, thereby providing guidance for subsequent drug release, and finally realizing efficient radiotherapy sensitization.

The invention relates to a liquid chromatography method for simultaneously measuring 10 polyphenol substances in fruits and vegetables, which comprises the following steps: S1, weighing a sample to be measured, adding an ethanol aqueous solution containing hydrochloric acid, carrying out ultrasonic extraction, and centrifuging to obtain supernate; repeating once, combining the supernate, carrying out nitrogen blowing or rotary evaporation until the supernate is nearly dry, redissolving with a methanol aqueous solution, filtering, and standing for measurement on a machine; s2, preparing a standard solution of 10 polyphenol substances by taking methanol as a solvent; s3, determining the sample to be detected and the standard solution by using a high performance liquid chromatograph; and S4, calculating the content of the polyphenol substance according to the retention time, the peak area and the standard curve. The method provided by the invention can be used for determining the content of ten polyphenol substances including gallic acid, chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, catechin, epicatechin, quercetin, luteolin and kaempferide in the fruits and vegetables, has the characteristics of rapidness, accuracy and high sensitivity, and provides timely and accurate technical and data support for determination and monitoring of phytochemicals in the fruits and vegetables.