11results about How to "High sensitivity detection" patented technology

The embodiment of the invention belongs to the technical field of electrochemical gas sensors, and relates to a preparation method of a counter electrode of an NO sensor, which comprises the following steps: adding a polymer adhesive into an organic solvent to prepare a polymer adhesive mother solution; mixing manganese dioxide powder with the polymer adhesive mother liquor, and stirring to form uniform slurry; coating a substrate with the slurry, and performing drying treatment to form a counter electrode plate; and putting the counter electrode plate into an environment with reducing gas or inert gas to carry out high-temperature reduction treatment so as to improve the oxygen vacancy concentration, thereby obtaining the counter electrode of the NO sensor. The invention further provides an NO sensor and an NO detection method. The counter electrode of the NO sensor and the NO sensor prepared by the embodiment of the invention have the advantages of low cost and high anti-interference capability while keeping high oxygen reduction catalytic activity, and are particularly suitable for high-selectivity and high-sensitivity detection of NO gas in the fields of environmental monitoring, medical diagnosis and the like.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

This invention belongs to the field of medical testing technology, specifically relating to a lateral flow test strip for ultra-high sensitivity detection of pathogens and its application. The lateral flow test strip provided by this invention uses polydopamine nanoparticles modified with bacterial antibodies as signal probes. Multiple detection lines are set on the NC membrane, and the detection lines are obtained by scribing with a bacterial antibody solution as ink. This invention replaces Au NPs in classic LFIAs with PDA NPs. Relying on the high extinction coefficient, affinity for bacteria, and stability of antibody binding of PDA NPs, a two-order-of-magnitude improvement in sensitivity is achieved when using LFIAs to detect bacteria. Simultaneously, utilizing the high extinction coefficient of PDA NPs, their signals can be identified and statistically analyzed by ImageJ software even when invisible to the naked eye. Combined with multiple test strips, this improves target capture efficiency, thereby achieving highly sensitive detection of pathogenic bacteria.

This invention provides a rapid colorimetric reagent for the detection of manganese and iron ions. The reagent consists of carbon dots, a 3,3′,5,5′-tetramethylbenzidine solution, and a phosphate buffer solution. The reagent itself is a brown, transparent solution. When manganese ions are added to the 3,3′,5,5′-tetramethylbenzidine solution and phosphate buffer solution, it turns blue (visible to the naked eye) upon irradiation with a 365 nm UV lamp; upon contact with iron ions in the 3,3′,5,5′-tetramethylbenzidine solution, it turns dark green (visible to the naked eye). This reagent exhibits an extremely low detection limit and good selectivity. The detection process does not require complex analytical equipment, enabling rapid trace detection of manganese and iron ions. The reagent is simple to prepare, overcoming the shortcomings of cumbersome and expensive synthesis of manganese and iron ion detection probe molecules, and providing effective technical support for trace detection in food safety.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The application discloses a long-afterglow nano carrier and a preparation method and application thereof, and belongs to the field of biomedical materials. The long-afterglow nano carrier comprises a near-infrared luminescent center, an organic mesoporous silica layer and an autophagy inhibitor; the organic mesoporous silica layer is coated on the outer layer of the near-infrared luminescent center, and the autophagy inhibitor is loaded in the mesopores of the organic mesoporous silica layer; the near-infrared luminescent center comprises a transition metal element; the organic mesoporous silica layer is prepared by co-condensation of an organic silane precursor and an inorganic silicon precursor, wherein the organic silane precursor is an organic silane containing a tetrasulfide bond, and the inorganic silicon precursor is a silicate compound. The carrier can specifically respond to excessively high concentration of hydrogen peroxide in a tumor microenvironment, and accurately release the loaded autophagy inhibitor; the release process can be observed by positioning with the aid of a high signal-to-noise ratio afterglow signal, thereby providing guidance for subsequent drug release, and finally realizing efficient radiotherapy sensitization.

The invention is applicable to the technical field of synthesis and application of optical functional materials, and provides a high-water-solubility large-pi conjugated SO2 probe, the structure of the probe is D-(pi) nA, and a preparation method of the probe comprises the following steps: step 1, synthesis of an electron donor of the probe, step 2, synthesis of an electron acceptor of the probe, and step 3, synthesis of a probe TFM3-2. The SO2 probe has high selectivity, low detection limit and strong water solubility, and can be applied to on-site rapid detection of sulfite in food and detection of organism cell membranes and organism endogenous SO2.

The invention provides a spectrometer based on a single-pixel single-channel single-photon detector and a detection method, and the spectrometer comprises a dispersion module which broadens a to-be-detected broadband light beam in space; the light beam path control module is used for controlling the light beam after space broadening to perform one-dimensional movement on the surface of the single-photon detector so as to ensure that light with different wavelengths is sequentially irradiated on the single-photon detector; the single-photon detector is used for detecting components with different wavelengths, realizing horizontal detection of a single photon and outputting a detection signal to the time-to-digital converter; the time-to-digital converter is used for recording and converting photon signal time information into digital signals; and the control system is connected with the light beam path control module and the time-to-digital converter, establishes single-channel communication with the single-photon detector, and is used for controlling the motion state of the light beam in the whole detection process and recording the photon count of each wavelength so as to obtain the wavelength-intensity corresponding relation of the light beam to be detected. The invention has the advantages of high detection sensitivity, high resolution, simple system and low cost.

The application discloses a kind of RPA-Cas12a one-pot rapid detection system and application thereof, the system includes RPA premix and CRISPR-Cas12a premix;CRISPR-Cas12a premix includes ssDNA, Cas12a and crRNA;crRNA is the specific guide RNA sequence based on suboptimal PAM sequence design can guide Cas12a target recognition AHPND RPA product.This application designs specific crRNA, reduces the cleavage efficiency of Cas12a, makes RPA amplification process and CRISPR-Cas12a specific cleavage process simultaneously in a tube reaction, avoids the potential pollution risk of RPA amplification product transfer in two-step method and cumbersome steps, high sensitivity, good stability, provides high sensitivity, high accuracy, good stability, simple and fast AHPND on-site detection method for aquaculture industry.

The invention relates to a leaked material tracing method for a coal chemical process condensate recovery system, which comprises the following steps of: establishing a hydrocarbon fingerprint database of materials with leakage possibility in the coal chemical process condensate recovery system based on a gas chromatography technology, correcting a gas chromatogram of process condensate, and comparing the corrected gas chromatogram with the hydrocarbon fingerprint database; the leakage source is determined through a similarity algorithm, efficient traceability analysis of coal chemical process condensate leakage materials can be achieved, the method has the advantages of being accurate in traceability, high in detection sensitivity, wide in applicability and easy and convenient to operate, the method is suitable for different coal chemical process systems, and the accuracy and real-time performance of leakage detection are remarkably improved; and risks of system imbalance, resource waste and environmental pollution are reduced.

The application provides a biosensor based on a screen-printed electrode and a preparation method and application thereof. The preparation method comprises the following steps: a, depositing a silicon thin film transition layer on the surface of a working electrode of a screen-printed electrode by using a PECVD device; b, spin-coating a tin dioxide solution on the surface of the silicon thin film transition layer to form a tin dioxide layer; c, first performing hydrogen reduction on the surface of the tin dioxide layer, and then performing silicon nanowire growth to obtain a silicon nanowire layer; d, first performing ultraviolet ozone treatment on the silicon nanowire layer, then placing the silicon nanowire layer in an alcohol solution containing 3-aminopropyl triethoxysilane to perform condensation reaction, cleaning, then performing heating to enhance the coupling strength, and finally placing the silicon nanowire layer in a phosphate buffer solution containing a nucleic acid aptamer to fix the nucleic acid aptamer, so as to obtain the biosensor. The application prepares silicon nanowires on a screen-printed electrode, then fixes a nucleic acid aptamer, and constructs an electrochemical sensor for detecting Alzheimer's disease, and the electrochemical sensor has excellent conductivity, biocompatibility and detection sensitivity.