27results about How to "High Sensitivity Detection" patented technology

The invention discloses a hydroxyproline rapid detection molecularly imprinted electrochemical sensor as well as preparation and application thereof, and relates to the field of quality safety monitoring of medicinal plants. The preparation method of the molecularly imprinted electrochemical sensor comprises the following steps: preparing the Fc-MWCNTs composite material, preparing the Fc-MWCNTs / GCE, and preparing the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE. According to the invention, MWCNTs with good conductivity and Fc which has good stability and oxidation-reduction property and is used as an excellent electron transfer medium are used as electrode substrate materials; and the Fc-MWCNTs and the MWCNTs are covalently bonded through an amide reaction to prepare the composite material Fc-MWCNTs which has excellent electron transfer medium characteristics, low potential reproducibility and strong stability and can remarkably improve the electron transfer rate between the electrode surface and an electrolyte. The modified composite material Fc-MWCNTs is modified on the surface of GCE, so that the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE with high sensitivity, high selective recognition and fast detection is constructed, and the molecularly imprinted electrochemical sensor is used for detecting an index component Hyp of cornu cervi pantotrichum.

The embodiment of the invention belongs to the technical field of electrochemical gas sensors, and relates to a preparation method of a counter electrode of an NO sensor, which comprises the following steps: adding a polymer adhesive into an organic solvent to prepare a polymer adhesive mother solution; mixing manganese dioxide powder with the polymer adhesive mother liquor, and stirring to form uniform slurry; coating a substrate with the slurry, and performing drying treatment to form a counter electrode plate; and putting the counter electrode plate into an environment with reducing gas or inert gas to carry out high-temperature reduction treatment so as to improve the oxygen vacancy concentration, thereby obtaining the counter electrode of the NO sensor. The invention further provides an NO sensor and an NO detection method. The counter electrode of the NO sensor and the NO sensor prepared by the embodiment of the invention have the advantages of low cost and high anti-interference capability while keeping high oxygen reduction catalytic activity, and are particularly suitable for high-selectivity and high-sensitivity detection of NO gas in the fields of environmental monitoring, medical diagnosis and the like.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

The invention belongs to the technical field of biosensing and microelectronics, and relates to a preparation method and application of an extended gate field effect transistor for thrombin instant detection. The expanded gate field effect transistor is modified by the Prussian blue / platinum nanoparticle composite material, the electrochemical sensing performance of the field effect transistor is greatly improved, and the expanded gate field effect transistor prepared based on the composite material shows excellent anti-interference capability and recognition capability. The expanded gate field effect transistor has the advantages of being low in cost, easy to replace and the like, the preparation process is simple and controllable, large-scale and productization production is easy to achieve, the expanded gate field effect transistor based on the Prussian blue / platinum nanoparticles can achieve rapid recognition and instant detection of thrombin, the blank of thrombin detection products in the market at present is filled, and the application prospect is wide. And the detection efficiency of the blood coagulation index is greatly improved.

The present application relates to a kind of biological base micro needle sensor-based crop health nondestructive testing method, belong to agricultural information technology and biosensor field.The method is intelligently analyzed to crop surface microstructure by convolutional neural network, realize the accurate arrangement of microneedle array and customized nondestructive adaptation.Microneedle multimodal sensing unit can synchronously real-time acquisition crop internal multidimensional physiological parameters, and signal quality is improved using adaptive filtering and standardization processing.Combined with multivariate anomaly detection algorithm of self-supervised and contrast learning, health status is unsupervised clustering and early abnormal screening, and environmental and management information is fused, realize whole plant multiscale health evaluation, abnormal risk distribution analysis and dynamic traceability.System can intelligently output differentiated farm work suggestions, realize the high accuracy, nondestructive, real-time monitoring and intelligent management of crop health.

This invention belongs to the field of medical testing technology, specifically relating to a lateral flow test strip for ultra-high sensitivity detection of pathogens and its application. The lateral flow test strip provided by this invention uses polydopamine nanoparticles modified with bacterial antibodies as signal probes. Multiple detection lines are set on the NC membrane, and the detection lines are obtained by scribing with a bacterial antibody solution as ink. This invention replaces Au NPs in classic LFIAs with PDA NPs. Relying on the high extinction coefficient, affinity for bacteria, and stability of antibody binding of PDA NPs, a two-order-of-magnitude improvement in sensitivity is achieved when using LFIAs to detect bacteria. Simultaneously, utilizing the high extinction coefficient of PDA NPs, their signals can be identified and statistically analyzed by ImageJ software even when invisible to the naked eye. Combined with multiple test strips, this improves target capture efficiency, thereby achieving highly sensitive detection of pathogenic bacteria.

The invention belongs to the technical field of electrochemical detection, and particularly relates to an enrofloxacin aptamer sensor based on a screen-printed electrode and a rapid detection method. The preparation method comprises the following steps: firstly preparing a Fe / Co two-dimensional MOF nanosheet with a lamellar structure by using a room temperature stirring method, then incubating with an enrofloxacin aptamer to prepare a nanosheet / aptamer probe, further incubating with enrofloxacin, mixing with an acetic acid-sodium acetate buffer solution, o-phenylenediamine and H2O2 to form a catalytic reaction system, and by combining with a screen-printed electrode-differential pulse voltammetry, detecting the content of the enrofloxacin. The enrofloxacin concentration of a sample to be detected is rapidly tested by drawing a quantitative analysis standard working curve, and rapid, simple and high-sensitivity detection of enrofloxacin is realized. The method is simple and rapid to operate, does not need expensive reagents, large-scale instruments and professional operators, is high in sensitivity, and can realize on-site, rapid and high-sensitivity quantitative detection of enrofloxacin in aquatic products such as grass-roots fish and shrimps in combination with a portable electrochemical detector.

The present invention belongs to the field of antibody technology, and in particular, relates to anti-human granulocyte-macrophage colony stimulating factor antibodies, antibody pairs and their applications. The antibody is a first antibody or a second antibody, and the amino acid sequences of CDR1-3 on the light chain variable region of the first antibody are shown as SEQ ID NO.3-5, respectively, and the amino acid sequences of CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.8-10, respectively; the amino acid sequences of CDR1-3 on the light chain variable region of the second antibody are shown as SEQ ID NO.13-15, respectively, and the amino acid sequences of CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.18-20, respectively. The present invention provides antibodies with good specificity and high affinity for human GM-CSF, which can effectively improve the accuracy, reliability and sensitivity of immunoassay results, and provide a more accurate and reliable antibody tool for detecting GM-CSF protein levels.

This invention provides a rapid colorimetric reagent for the detection of manganese and iron ions. The reagent consists of carbon dots, a 3,3′,5,5′-tetramethylbenzidine solution, and a phosphate buffer solution. The reagent itself is a brown, transparent solution. When manganese ions are added to the 3,3′,5,5′-tetramethylbenzidine solution and phosphate buffer solution, it turns blue (visible to the naked eye) upon irradiation with a 365 nm UV lamp; upon contact with iron ions in the 3,3′,5,5′-tetramethylbenzidine solution, it turns dark green (visible to the naked eye). This reagent exhibits an extremely low detection limit and good selectivity. The detection process does not require complex analytical equipment, enabling rapid trace detection of manganese and iron ions. The reagent is simple to prepare, overcoming the shortcomings of cumbersome and expensive synthesis of manganese and iron ion detection probe molecules, and providing effective technical support for trace detection in food safety.

The application belongs to the technical field of immune detection, and particularly relates to a quantum dot labeled testosterone propionate direct competition fluorescence immune detection method. The application prepares a monoclonal antibody, and couples CdSe / ZnS (core / shell) quantum dots with the prepared anti-TP monoclonal antibody to synthesize a quantum dot (QDs) labeled anti-TP-mAb-QDs immune fluorescence probe, and construct a quantum dot labeled testosterone propionate direct competition fluorescence immune detection system for detecting testosterone propionate. The method for detecting testosterone propionate by the quantum dot labeled direct competition fluorescence immune detection method is simple in operation, and can detect the residual content of testosterone propionate in a sample to be detected without adding a chromogenic substance. The operation and reaction can be completed in one step. The method is simple, efficient, sensitive, low in detection cost, and can be applied to rapid detection of TP residues in feed, and has good accuracy.

The invention discloses a double-shaft type optical speed detector and a calibration method, and particularly relates to the technical field of detectors. The double-shaft type optical speed detector comprises a double-optical-path detection module, a sample adaptation module, a speckle manufacturing and detecting module, a data processing and calibration module and a display and control module. According to the double-shaft type optical speed detector and the calibration method, the light path building process is simplified through the arrangement of the integrated light path module, a user can carry out detection only through simple operation, the operation convenience is improved, the detection capacity for sample characteristic changes is effectively enhanced through the arrangement of the double-light-path detection module, and the detection accuracy is improved. The high-sensitivity detection is realized, the sample adaptation module is arranged to adapt to various samples, the universality of the device is improved, and meanwhile, the speckle manufacturing and detection module is arranged to realize high-quality and high-adaptability speckle manufacturing and detection. And the data processing and calibration module and the display and control module ensure the accuracy of data and the intuition of result display.

The invention relates to the technical field of electrochemistry, in particular to a preparation method and application of a copper-silver bimetal organic framework compound / rhodium electrode for electrochemically detecting total nitrogen, and the preparation method comprises the following steps: A, preparing terephthalic acid, silver nitrate and a solvent into a mixed solution, the foamy copper is placed in the mixed solution for a hydrothermal reaction, and a copper-silver bimetal organic framework compound is obtained; and B, forming a rhodium film layer outside the copper-silver bimetal organic framework compound in the step A, and finally annealing and removing impurities to obtain the copper-silver bimetal organic framework compound / rhodium electrode material, when the electrode is applied to total nitrogen detection, the electrode has stronger volt-ampere response to nitric acid, is good in stability, and can realize high-sensitivity and rapid detection of total nitrogen in a water body.

The invention discloses a method and post-treatment fluid for reducing squamous cell carcinoma antigen detection background, and is characterized in that the method comprises the following steps: step 1, adding a sample, magnetic beads containing a coated antibody, a sample diluent and a labeled antibody labeled with acridinium ester, incubating, and executing a cleaning step; step 2, adding a post-treatment liquid, incubating, and executing a cleaning step; step 3, adding a substrate solution, entering a photometric module, and measuring the number of photons; wherein the post-treatment liquid comprises the following raw material components: a basic buffer solution, a nonionic surfactant, an alkali metal chloride, a metal ion chelate and an anionic surfactant. The method provided by the invention is simple and convenient to operate, and can improve the detection accuracy and maintain high sensitivity.

The invention discloses a wind driven generator transmission chain vibration detection method based on an echo wall mode microcavity, and relates to the technical field of wind power generation. The wind driven generator transmission chain vibration detection method comprises the following steps: guiding a laser light source into the echo wall mode microcavity based on an optical fiber coupling technology; a vibration response signal at the position of a transmission chain of the wind driven generator is obtained by using the echo wall mode microcavity; and converting the vibration response signal into a digital signal by using a signal processing technology, analyzing and processing the digital signal by combining a digital signal processor, and obtaining vibration information at the position of the transmission chain of the wind driven generator based on a processing result. According to the wind driven generator transmission chain vibration detection method based on the echo wall mode microsphere cavity, the vibration condition of the wind driven generator transmission chain can be detected and analyzed in a high-precision and high-sensitivity mode, and meanwhile the method has the advantages of being capable of resisting interference and adapting to severe environments.

The invention belongs to the technical field of feed detection, and specifically relates to a rapid detection method for pesticide, veterinary drug and fungal toxin residues in feed, comprising the following steps: using a mixed extract of methanol, acetonitrile and isopropanol to extract a sample under alkaline conditions and acidic conditions in turn, mixing, solid phase extraction adsorbent (including N-vinylbenzene modified ferroferric oxide functionalized magnetic nano-adsorbent material and octadecyl silica gel C 18 After purification by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) in multiple reaction monitoring scanning mode, quantification is performed by external standard method or internal standard method. It can quickly and accurately detect 420 pesticides, 163 veterinary drugs and their metabolites and 13 mycotoxins in feed at the same time, with a quantitative limit of 0.01 μg / kg to 50 μg / kg, which is of great significance for ensuring food safety, public health and the sustainable development of animal husbandry.

The invention discloses a fluorescent self-early-warning anticorrosive coating as well as a preparation method and application thereof. The coating comprises epoxy resin coupled with probe molecules. The fluorescence emission wavelength of the probe molecule is located in a near-infrared region, and the fluorescence intensity can be enhanced under an acidic condition, so that the detection reliability of the self-early-warning anticorrosive coating and the detection depth of an early-warning signal are improved; according to the acidic pH response type fluorescent self-early-warning anticorrosive coating disclosed by the invention, coating cracks in different shapes can be accurately detected; the acidic pH-responsive fluorescent self-early-warning anticorrosive coating provided by the technical scheme of the invention has good stability, can perform long-time early warning on the anticorrosive coating, and provides long-term protection for ships.

The invention relates to the field of optical fiber sensing and the field of deep learning, in particular to an air drum structure and a low-frequency acoustic signal detection device and method based on the air drum structure, which combine the advantages of an F-P cavity formed by double-fiber Bragg gratings, a conical fiber and the air drum structure and fully play the advantages of simple structure, high sensitivity, easy multiplexing and the like of the optical F-P cavity. Round diaphragms with different areas and different corresponding resonant frequencies are prepared by considering the characteristic that the fiber Bragg grating only reflects specific wavelength and the natural high sensitivity characteristic of the conical fiber and combining the narrow-band filtering advantage of the round diaphragms, so that the empty drum structure detects sound waves with different frequencies, and the detection accuracy is improved. The frequency of the external sound wave can be directly obtained without additional Fourier transform and filtering operation, and intelligent autonomous identification of the target sound signal is realized; compared with a traditional low-frequency sound signal sensing system, the system is more sensitive, compact and efficient, and has the advantages of narrow-band filtering, real-time spectrum extraction, autonomous target recognition and the like.

The invention relates to the field of chimeric cell proportion detection, in particular to a method for highly sensitively detecting the proportion of chimeric cells after bone marrow transplantation. Aiming at the condition that a bone marrow transplantation donor and a patient are heterosexual, the method comprises the following steps: firstly, selecting a gender specific gene as a target, then analyzing the transcriptional level of the gender specific expression gene in a blood sample of the patient before and after transplantation through a real-time fluorescence quantification method, and finally calculating to obtain the residual proportion of the patient cells. The detection method provided by the invention can be used for evaluating the chimeric state of a heterologous hematopoietic stem cell transplantation patient and monitoring the implantation state, relapse or MRD of donor stem cells in a receptor so as to guide clinics to take corresponding treatment measures in time.

The application discloses a long-afterglow nano carrier and a preparation method and application thereof, and belongs to the field of biomedical materials. The long-afterglow nano carrier comprises a near-infrared luminescent center, an organic mesoporous silica layer and an autophagy inhibitor; the organic mesoporous silica layer is coated on the outer layer of the near-infrared luminescent center, and the autophagy inhibitor is loaded in the mesopores of the organic mesoporous silica layer; the near-infrared luminescent center comprises a transition metal element; the organic mesoporous silica layer is prepared by co-condensation of an organic silane precursor and an inorganic silicon precursor, wherein the organic silane precursor is an organic silane containing a tetrasulfide bond, and the inorganic silicon precursor is a silicate compound. The carrier can specifically respond to excessively high concentration of hydrogen peroxide in a tumor microenvironment, and accurately release the loaded autophagy inhibitor; the release process can be observed by positioning with the aid of a high signal-to-noise ratio afterglow signal, thereby providing guidance for subsequent drug release, and finally realizing efficient radiotherapy sensitization.

The invention relates to a liquid chromatography method for simultaneously measuring 10 polyphenol substances in fruits and vegetables, which comprises the following steps: S1, weighing a sample to be measured, adding an ethanol aqueous solution containing hydrochloric acid, carrying out ultrasonic extraction, and centrifuging to obtain supernate; repeating once, combining the supernate, carrying out nitrogen blowing or rotary evaporation until the supernate is nearly dry, redissolving with a methanol aqueous solution, filtering, and standing for measurement on a machine; s2, preparing a standard solution of 10 polyphenol substances by taking methanol as a solvent; s3, determining the sample to be detected and the standard solution by using a high performance liquid chromatograph; and S4, calculating the content of the polyphenol substance according to the retention time, the peak area and the standard curve. The method provided by the invention can be used for determining the content of ten polyphenol substances including gallic acid, chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, catechin, epicatechin, quercetin, luteolin and kaempferide in the fruits and vegetables, has the characteristics of rapidness, accuracy and high sensitivity, and provides timely and accurate technical and data support for determination and monitoring of phytochemicals in the fruits and vegetables.

The invention is applicable to the technical field of synthesis and application of optical functional materials, and provides a high-water-solubility large-pi conjugated SO2 probe, the structure of the probe is D-(pi) nA, and a preparation method of the probe comprises the following steps: step 1, synthesis of an electron donor of the probe, step 2, synthesis of an electron acceptor of the probe, and step 3, synthesis of a probe TFM3-2. The SO2 probe has high selectivity, low detection limit and strong water solubility, and can be applied to on-site rapid detection of sulfite in food and detection of organism cell membranes and organism endogenous SO2.

The invention provides a spectrometer based on a single-pixel single-channel single-photon detector and a detection method, and the spectrometer comprises a dispersion module which broadens a to-be-detected broadband light beam in space; the light beam path control module is used for controlling the light beam after space broadening to perform one-dimensional movement on the surface of the single-photon detector so as to ensure that light with different wavelengths is sequentially irradiated on the single-photon detector; the single-photon detector is used for detecting components with different wavelengths, realizing horizontal detection of a single photon and outputting a detection signal to the time-to-digital converter; the time-to-digital converter is used for recording and converting photon signal time information into digital signals; and the control system is connected with the light beam path control module and the time-to-digital converter, establishes single-channel communication with the single-photon detector, and is used for controlling the motion state of the light beam in the whole detection process and recording the photon count of each wavelength so as to obtain the wavelength-intensity corresponding relation of the light beam to be detected. The invention has the advantages of high detection sensitivity, high resolution, simple system and low cost.

The application discloses a preparation method of a hierarchical structure anti-fouling polypeptide chip and application thereof, and the preparation method comprises the following steps: flowing a zwitterionic polypeptide mixed solution through a chip to form hierarchical structure polypeptides on the surface of the chip; and fixing recognition molecules on the surface of the hierarchical structure polypeptides by using an activated ester method to obtain the hierarchical structure anti-fouling polypeptide chip. The application comprises the following steps: measuring the surface plasmon resonance sensor signal response value of a test solution / standard solution, drawing a standard curve; measuring the surface plasmon resonance sensor signal response value of a sample to be measured, and substituting the response value into the standard curve to obtain / calculating the concentration of the sample to be measured. The application constructs polypeptides with different hierarchical structures on the surface of the chip, and improves the anti-non-specific adsorption interference ability of the surface plasmon resonance sensor by using the anti-fouling characteristics of the hierarchical structure polypeptides and the binding characteristics between antigens and antibodies, so that the problem of non-specific adsorption of proteins in the traditional surface plasmon resonance technology is solved.

The application discloses a kind of RPA-Cas12a one-pot rapid detection system and application thereof, the system includes RPA premix and CRISPR-Cas12a premix;CRISPR-Cas12a premix includes ssDNA, Cas12a and crRNA;crRNA is the specific guide RNA sequence based on suboptimal PAM sequence design can guide Cas12a target recognition AHPND RPA product.This application designs specific crRNA, reduces the cleavage efficiency of Cas12a, makes RPA amplification process and CRISPR-Cas12a specific cleavage process simultaneously in a tube reaction, avoids the potential pollution risk of RPA amplification product transfer in two-step method and cumbersome steps, high sensitivity, good stability, provides high sensitivity, high accuracy, good stability, simple and fast AHPND on-site detection method for aquaculture industry.

The invention relates to a leaked material tracing method for a coal chemical process condensate recovery system, which comprises the following steps of: establishing a hydrocarbon fingerprint database of materials with leakage possibility in the coal chemical process condensate recovery system based on a gas chromatography technology, correcting a gas chromatogram of process condensate, and comparing the corrected gas chromatogram with the hydrocarbon fingerprint database; the leakage source is determined through a similarity algorithm, efficient traceability analysis of coal chemical process condensate leakage materials can be achieved, the method has the advantages of being accurate in traceability, high in detection sensitivity, wide in applicability and easy and convenient to operate, the method is suitable for different coal chemical process systems, and the accuracy and real-time performance of leakage detection are remarkably improved; and risks of system imbalance, resource waste and environmental pollution are reduced.

The application provides a biosensor based on a screen-printed electrode and a preparation method and application thereof. The preparation method comprises the following steps: a, depositing a silicon thin film transition layer on the surface of a working electrode of a screen-printed electrode by using a PECVD device; b, spin-coating a tin dioxide solution on the surface of the silicon thin film transition layer to form a tin dioxide layer; c, first performing hydrogen reduction on the surface of the tin dioxide layer, and then performing silicon nanowire growth to obtain a silicon nanowire layer; d, first performing ultraviolet ozone treatment on the silicon nanowire layer, then placing the silicon nanowire layer in an alcohol solution containing 3-aminopropyl triethoxysilane to perform condensation reaction, cleaning, then performing heating to enhance the coupling strength, and finally placing the silicon nanowire layer in a phosphate buffer solution containing a nucleic acid aptamer to fix the nucleic acid aptamer, so as to obtain the biosensor. The application prepares silicon nanowires on a screen-printed electrode, then fixes a nucleic acid aptamer, and constructs an electrochemical sensor for detecting Alzheimer's disease, and the electrochemical sensor has excellent conductivity, biocompatibility and detection sensitivity.

The invention discloses a photosensitive mass spectrum probe as well as a preparation method and application thereof in mass spectrum detection, and belongs to the technical field of organic chemistry and analytical chemistry. According to the invention, a thioxanthone oxime benzenesulfonate structure is used as a basis and is bonded with an active reaction group to construct a high-efficiency photosensitive mass spectrum probe, so that rapid labeling of an analyte and online high-efficiency photolysis of a labeled product can be realized, and the labeled product with a sulfonic acid group is released. The invention also provides a preparation method of the photosensitive mass spectrum probe, the photosensitive mass spectrum probe with a target structure is synthesized in a simple and feasible manner, and the preparation method has good guidance and feasibility. When the photosensitive mass spectrum probe is applied to metabolite labeling and mass spectrum detection, the detection sensitivity is improved, the ion inhibition effect caused by excessive labeling reagents is reduced, high-flux and high-sensitivity detection analysis of low-ionization-efficiency analytes is realized, and the probe has a wide prospect.