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Combined markers, kit and system for diagnosis of polycystic ovarian syndrome

A technology of polycystic ovary and markers, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as high cost and complicated diagnosis of polycystic ovary syndrome

Inactive Publication Date: 2015-07-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a combination of small molecule metabolites in the auxiliary diagnosis of polycystic ovary syndrome for the clinical problems of complex diagnosis and high cost of polycystic ovary syndrome, and to provide small molecules that can be used in the above combination Metabolite analysis and detection methods

Method used

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  • Combined markers, kit and system for diagnosis of polycystic ovarian syndrome
  • Combined markers, kit and system for diagnosis of polycystic ovarian syndrome
  • Combined markers, kit and system for diagnosis of polycystic ovarian syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Serum Sample Collection

[0071] Before collection, all volunteers included in the study signed the informed consent.

[0072] 50 patients with polycystic ovary syndrome (including 25 obese (ie BMI>23) polycystic ovary syndrome patients and 25 non-obese polycystic ovary syndrome patients) on the 3-5th day of natural menstrual cycle or progesterone withdrawal Or when there were no dominant follicles in both ovaries, blood was collected from 35 normal people (including 15 obese normal people and 20 normal weight normal people) on the 3rd to 5th day of menstruation. All the blood samples were taken the next morning after fasting for more than 8 hours. The collected blood samples were left to stand for 30 minutes, centrifuged at 3000rpm / min for 10 minutes, and the serum was collected and stored in a -80°C refrigerator for later use.

[0073] 2. Analysis method

[0074] 2.1 Serum sample pretreatment

[0075] Blood samples were thawed at 4°C, 100 μL of serum samples we...

Embodiment 2

[0086] Another batch of serum samples was collected to validate the combined markers of PCOS, including 12 normal weight controls, 7 obese controls, 15 normal weight PCOS patients, 12 obese polycystic ovary syndrome patients, and 12 obese controls. patients with cystic ovary syndrome.

[0087] 1. Serum Sample Collection

[0088] The sample collection method is the same as in Example 1.

[0089] 2. Analysis method

[0090] 2.1 The serum sample pretreatment method is the same as that in Example 1.

[0091] 2.2 Ultra-high performance liquid chromatography mass spectrometry analysis

[0092] (1) Liquid phase conditions: Waters ACQUITY-Ultra Performance Liquid Chromatography system (Milford, Milford, USA) was used as the liquid chromatography analysis instrument. The chromatographic column is Waters 2.1×100nm, 1.8μm, HSS T3 column (Waters, Milford, USA); mobile phase A is 6.5mM ammonium bicarbonate aqueous solution, mobile phase B is 6.5mM ammonium bicarbonate methanol / water so...

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Abstract

The invention relates to a novel use of serum sample micromolecule metabolites such as oleic acid, stearic acid, eicosatrienoic acid, dehydroepiandrosterone sulfate, glycosylated phenylalanine and uridine as combined markers in preparation of a kit for diagnosis of polycystic ovarian syndrome of subjects. The invention also relates to a kit of diagnosis of polycystic ovarian syndrome of subjects. The kit is used for detecting respective concentrations of the combined markers in a serum sample of a female subject, calculating combined marker variable based on a binary logistic regression equation, and determining if the subject suffers from polycystic ovarian syndrome based on the determined intercept point value. The kit can realizes high sensitivity and high efficiency detection of the micromolecular metabolites and has the characteristics of low detection cost and good repeatability. Through combined use of the micromolecular metabolites, the kit can be used in auxiliary diagnosis of different symptoms of polycystic ovarian syndrome.

Description

technical field [0001] The present invention relates to novel uses of known metabolites. Specifically, the present invention relates to the combination of oleic acid, stearic acid, eicosatrienoic acid, dehydroepiandrosterone sulfide, glycosylated phenylalanine and uridine in the serum for the diagnosis of polycystic ovary syndrome. markers, and relates to kits and systems for detecting polycystic ovary syndrome in a subject. Background technique [0002] Polycystic ovary syndrome (Polycystic ovary syndrome) is the most common disease in adolescents and women of childbearing age, with an incidence rate of 5% to 10%. Polycystic ovary syndrome can lead to female infertility, increase the risk of cardiovascular disease, diabetes, etc. Polycystic ovary syndrome is one of the most complex and difficult diseases in the field of gynecological endocrinology. The etiology is unknown, and the mechanism of occurrence and development has not yet been elucidated. It involves the combine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 许国旺李艳杰赵欣捷侯丽辉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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